EA005141B1 - Способ детекции нуклеотидного полиморфизма - Google Patents

Способ детекции нуклеотидного полиморфизма Download PDF

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Publication number
EA005141B1
EA005141B1 EA200300880A EA200300880A EA005141B1 EA 005141 B1 EA005141 B1 EA 005141B1 EA 200300880 A EA200300880 A EA 200300880A EA 200300880 A EA200300880 A EA 200300880A EA 005141 B1 EA005141 B1 EA 005141B1
Authority
EA
Eurasian Patent Office
Prior art keywords
nucleotide
base
nucleic acid
target nucleic
nuclease
Prior art date
Application number
EA200300880A
Other languages
English (en)
Russian (ru)
Other versions
EA200300880A1 (ru
Inventor
Хироаки Сагава
Еидзи Кобаяси
Икуносин Като
Original Assignee
Такара Био Инк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Такара Био Инк. filed Critical Такара Био Инк.
Publication of EA200300880A1 publication Critical patent/EA200300880A1/ru
Publication of EA005141B1 publication Critical patent/EA005141B1/ru

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EA200300880A 2001-02-15 2002-02-14 Способ детекции нуклеотидного полиморфизма EA005141B1 (ru)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
JP2001039268 2001-02-15
JP2001040721 2001-02-16
JP2001101055 2001-03-30
JP2001177381 2001-06-12
JP2001290384 2001-09-25
JP2001338440 2001-11-02
JP2001368929 2001-12-03
PCT/JP2002/001222 WO2002064833A1 (en) 2001-02-15 2002-02-14 Method of detecting nucleotide polymorphism

Publications (2)

Publication Number Publication Date
EA200300880A1 EA200300880A1 (ru) 2004-02-26
EA005141B1 true EA005141B1 (ru) 2004-12-30

Family

ID=27567022

Family Applications (1)

Application Number Title Priority Date Filing Date
EA200300880A EA005141B1 (ru) 2001-02-15 2002-02-14 Способ детекции нуклеотидного полиморфизма

Country Status (9)

Country Link
US (1) US7135291B2 (https=)
EP (1) EP1367136A4 (https=)
JP (3) JP3681729B2 (https=)
KR (1) KR100809949B1 (https=)
CN (1) CN100351393C (https=)
AU (1) AU2002232177B2 (https=)
CA (1) CA2438574C (https=)
EA (1) EA005141B1 (https=)
WO (1) WO2002064833A1 (https=)

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WO2014143228A1 (en) * 2013-03-15 2014-09-18 Integrated Dna Technologies, Inc. Rnase h-based assays utilizing modified rna monomers
US9434988B2 (en) 2008-04-30 2016-09-06 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
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JPWO2010026933A1 (ja) * 2008-09-03 2012-02-02 タカラバイオ株式会社 Ｒｎａ検出用組成物
ES2527653T3 (es) 2008-10-20 2015-01-28 F. Hoffmann-La Roche Ag Amplificación específica de alelo usando un cebador con un nucleótido modificado
WO2011060014A1 (en) * 2009-11-13 2011-05-19 Integrated Dna Technologies, Inc. Small rna detection assays
US9238832B2 (en) 2009-12-11 2016-01-19 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids
US8614071B2 (en) 2009-12-11 2013-12-24 Roche Molecular Systems, Inc. Preferential amplification of mRNA over DNA using chemically modified primers
CA2881200A1 (en) 2012-02-14 2013-08-22 Great Basin Scientific Methods of isothermal amplification using blocked primers
CN105189746B (zh) 2013-03-14 2018-12-04 宝生物工程株式会社 用于使用耐热的错配内切核酸酶的方法
CA3207128A1 (en) 2013-11-14 2015-05-21 Integrated Dna Technologies, Inc. Dna polymerase mutants having enhanced template discrimination activity
EP3825411A1 (en) 2014-06-18 2021-05-26 Clear Gene, Inc. Methods, compositions, and devices for rapid analysis of biological markers
CN107002067A (zh) 2014-09-11 2017-08-01 宝生物工程株式会社 利用热稳定性错配内切核酸酶的方法
US9909169B2 (en) 2014-12-17 2018-03-06 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids using blocking oligonucleotides for wild type suppression
JP6914831B2 (ja) * 2015-03-20 2021-08-04 タカラバイオ株式会社 標的核酸の高感度検出方法
WO2017106790A1 (en) 2015-12-18 2017-06-22 Clear Gene, Inc. Methods, compositions, kits and devices for rapid analysis of biological markers
US12275994B2 (en) 2017-06-22 2025-04-15 Clear Gene, Inc. Methods and compositions for the analysis of cancer biomarkers
CN108148895A (zh) * 2017-12-29 2018-06-12 星阵（广州）基因科技有限公司 用于检测突变的人akt1基因的引物、试剂盒及方法
AU2022216246A1 (en) * 2021-02-02 2023-09-14 Rhodx, Inc. Synthetic polynucleotides and methods for selectively amplifying alleles
CN113215163B (zh) * 2021-05-12 2023-05-12 苏州海苗生物科技有限公司 一种特异性扩增目的基因的分子锁及应用

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Also Published As

Publication number Publication date
EA200300880A1 (ru) 2004-02-26
CN100351393C (zh) 2007-11-28
WO2002064833A1 (en) 2002-08-22
CN1524127A (zh) 2004-08-25
JPWO2002064833A1 (ja) 2004-06-17
JP3681729B2 (ja) 2005-08-10
US20040137451A1 (en) 2004-07-15
KR20030074797A (ko) 2003-09-19
CA2438574C (en) 2009-08-11
EP1367136A1 (en) 2003-12-03
JP2004298200A (ja) 2004-10-28
US7135291B2 (en) 2006-11-14
KR100809949B1 (ko) 2008-03-06
JP2005245465A (ja) 2005-09-15
AU2002232177B2 (en) 2006-11-09
CA2438574A1 (en) 2002-08-22
EP1367136A4 (en) 2005-01-12

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