CN100351393C - 核苷酸多态性的检测方法 - Google Patents

核苷酸多态性的检测方法 Download PDF

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Publication number
CN100351393C
CN100351393C CNB028079310A CN02807931A CN100351393C CN 100351393 C CN100351393 C CN 100351393C CN B028079310 A CNB028079310 A CN B028079310A CN 02807931 A CN02807931 A CN 02807931A CN 100351393 C CN100351393 C CN 100351393C
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nucleotide
nucleic acid
base
target nucleic
nucleotides
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Chinese (zh)
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CN1524127A (zh
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佐川裕章
小林英二
加藤郁之进
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Takara Bio Inc
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Takara Bio Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CNB028079310A 2001-02-15 2002-02-14 核苷酸多态性的检测方法 Expired - Fee Related CN100351393C (zh)

Applications Claiming Priority (14)

Application Number Priority Date Filing Date Title
JP39268/2001 2001-02-15
JP2001039268 2001-02-15
JP40721/2001 2001-02-16
JP2001040721 2001-02-16
JP101055/2001 2001-03-30
JP2001101055 2001-03-30
JP2001177381 2001-06-12
JP177381/2001 2001-06-12
JP290384/2001 2001-09-25
JP2001290384 2001-09-25
JP2001338440 2001-11-02
JP338440/2001 2001-11-02
JP2001368929 2001-12-03
JP368929/2001 2001-12-03

Publications (2)

Publication Number Publication Date
CN1524127A CN1524127A (zh) 2004-08-25
CN100351393C true CN100351393C (zh) 2007-11-28

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CNB028079310A Expired - Fee Related CN100351393C (zh) 2001-02-15 2002-02-14 核苷酸多态性的检测方法

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US (1) US7135291B2 (https=)
EP (1) EP1367136A4 (https=)
JP (3) JP3681729B2 (https=)
KR (1) KR100809949B1 (https=)
CN (1) CN100351393C (https=)
AU (1) AU2002232177B2 (https=)
CA (1) CA2438574C (https=)
EA (1) EA005141B1 (https=)
WO (1) WO2002064833A1 (https=)

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US10227641B2 (en) 2008-04-30 2019-03-12 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
US8911948B2 (en) * 2008-04-30 2014-12-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
WO2014143228A1 (en) * 2013-03-15 2014-09-18 Integrated Dna Technologies, Inc. Rnase h-based assays utilizing modified rna monomers
US9434988B2 (en) 2008-04-30 2016-09-06 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
DK2279263T3 (da) 2008-04-30 2013-11-11 Integrated Dna Tech Inc Rnase-h-baserede assays med anvendelse af modificerede rna-monomerer
JPWO2010026933A1 (ja) * 2008-09-03 2012-02-02 タカラバイオ株式会社 Ｒｎａ検出用組成物
ES2527653T3 (es) 2008-10-20 2015-01-28 F. Hoffmann-La Roche Ag Amplificación específica de alelo usando un cebador con un nucleótido modificado
WO2011060014A1 (en) * 2009-11-13 2011-05-19 Integrated Dna Technologies, Inc. Small rna detection assays
US9238832B2 (en) 2009-12-11 2016-01-19 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids
US8614071B2 (en) 2009-12-11 2013-12-24 Roche Molecular Systems, Inc. Preferential amplification of mRNA over DNA using chemically modified primers
CA2881200A1 (en) 2012-02-14 2013-08-22 Great Basin Scientific Methods of isothermal amplification using blocked primers
CN105189746B (zh) 2013-03-14 2018-12-04 宝生物工程株式会社 用于使用耐热的错配内切核酸酶的方法
CA3207128A1 (en) 2013-11-14 2015-05-21 Integrated Dna Technologies, Inc. Dna polymerase mutants having enhanced template discrimination activity
EP3825411A1 (en) 2014-06-18 2021-05-26 Clear Gene, Inc. Methods, compositions, and devices for rapid analysis of biological markers
CN107002067A (zh) 2014-09-11 2017-08-01 宝生物工程株式会社 利用热稳定性错配内切核酸酶的方法
US9909169B2 (en) 2014-12-17 2018-03-06 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids using blocking oligonucleotides for wild type suppression
JP6914831B2 (ja) * 2015-03-20 2021-08-04 タカラバイオ株式会社 標的核酸の高感度検出方法
WO2017106790A1 (en) 2015-12-18 2017-06-22 Clear Gene, Inc. Methods, compositions, kits and devices for rapid analysis of biological markers
US12275994B2 (en) 2017-06-22 2025-04-15 Clear Gene, Inc. Methods and compositions for the analysis of cancer biomarkers
CN108148895A (zh) * 2017-12-29 2018-06-12 星阵（广州）基因科技有限公司 用于检测突变的人akt1基因的引物、试剂盒及方法
AU2022216246A1 (en) * 2021-02-02 2023-09-14 Rhodx, Inc. Synthetic polynucleotides and methods for selectively amplifying alleles
CN113215163B (zh) * 2021-05-12 2023-05-12 苏州海苗生物科技有限公司 一种特异性扩增目的基因的分子锁及应用

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WO1992002638A1 (en) * 1990-08-06 1992-02-20 F. Hoffmann-La Roche Ag Homogeneous assay system
US5660988A (en) * 1993-11-17 1997-08-26 Id Biomedical Corporation Cycling probe cleavage detection of nucleic acid sequences
EP0705905A2 (de) * 1994-07-16 1996-04-10 Roche Diagnostics GmbH Verfahren zum sensitiven Nachweis von Nukleinsäuren
JP2001136965A (ja) * 1999-11-12 2001-05-22 Takara Shuzo Co Ltd 核酸配列の増幅方法

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Publication number Publication date
EA005141B1 (ru) 2004-12-30
EA200300880A1 (ru) 2004-02-26
WO2002064833A1 (en) 2002-08-22
CN1524127A (zh) 2004-08-25
JPWO2002064833A1 (ja) 2004-06-17
JP3681729B2 (ja) 2005-08-10
US20040137451A1 (en) 2004-07-15
KR20030074797A (ko) 2003-09-19
CA2438574C (en) 2009-08-11
EP1367136A1 (en) 2003-12-03
JP2004298200A (ja) 2004-10-28
US7135291B2 (en) 2006-11-14
KR100809949B1 (ko) 2008-03-06
JP2005245465A (ja) 2005-09-15
AU2002232177B2 (en) 2006-11-09
CA2438574A1 (en) 2002-08-22
EP1367136A4 (en) 2005-01-12

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