DK175091B1 - Fremgangsmåde til aktivering af genteknologisk fremstillede, heterologe, disulfidbro-holdige eukaryotiske proteiner efter ekspression i prokaryotiske celler - Google Patents

Fremgangsmåde til aktivering af genteknologisk fremstillede, heterologe, disulfidbro-holdige eukaryotiske proteiner efter ekspression i prokaryotiske celler Download PDF

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Publication number
DK175091B1
DK175091B1 DK198703203A DK320387A DK175091B1 DK 175091 B1 DK175091 B1 DK 175091B1 DK 198703203 A DK198703203 A DK 198703203A DK 320387 A DK320387 A DK 320387A DK 175091 B1 DK175091 B1 DK 175091B1
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Denmark
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process according
mol
mmol
concentration
reactivation
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DK198703203A
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Danish (da)
English (en)
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DK320387D0 (da
DK320387A (da
Inventor
Rainer Rudolph
Stephan Fischer
Ralf Mattes
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Roche Diagnostics Gmbh
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Publication of DK320387D0 publication Critical patent/DK320387D0/da
Publication of DK320387A publication Critical patent/DK320387A/da
Priority to DK200001897A priority Critical patent/DK175109B1/da
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Publication of DK175091B1 publication Critical patent/DK175091B1/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/565—IFN-beta
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Mechanical Treatment Of Semiconductor (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Lubricants (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
DK198703203A 1985-10-23 1987-06-23 Fremgangsmåde til aktivering af genteknologisk fremstillede, heterologe, disulfidbro-holdige eukaryotiske proteiner efter ekspression i prokaryotiske celler DK175091B1 (da)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DK200001897A DK175109B1 (da) 1985-10-23 2000-12-18 Fremgangsmåde til aktivering af genteknologisk fremstillede, heterologe, disulfidbroer indeholdende eukaryotiske proteiner efter ekspression i prokaryotiske celler

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE3537708 1985-10-23
DE19853537708 DE3537708A1 (de) 1985-10-23 1985-10-23 Verfahren zur aktivierung von t-pa nach expression in prokaryonten
PCT/EP1986/000610 WO1987002673A2 (en) 1985-10-23 1986-10-23 Process for activating heterologous, eucaryotic proteins genetically engineered and presenting disulphide bridges after their expression in procaryotic cells
EP8600610 1986-10-23

Publications (3)

Publication Number Publication Date
DK320387D0 DK320387D0 (da) 1987-06-23
DK320387A DK320387A (da) 1987-06-23
DK175091B1 true DK175091B1 (da) 2004-05-24

Family

ID=6284269

Family Applications (2)

Application Number Title Priority Date Filing Date
DK198703203A DK175091B1 (da) 1985-10-23 1987-06-23 Fremgangsmåde til aktivering af genteknologisk fremstillede, heterologe, disulfidbro-holdige eukaryotiske proteiner efter ekspression i prokaryotiske celler
DK200001897A DK175109B1 (da) 1985-10-23 2000-12-18 Fremgangsmåde til aktivering af genteknologisk fremstillede, heterologe, disulfidbroer indeholdende eukaryotiske proteiner efter ekspression i prokaryotiske celler

Family Applications After (1)

Application Number Title Priority Date Filing Date
DK200001897A DK175109B1 (da) 1985-10-23 2000-12-18 Fremgangsmåde til aktivering af genteknologisk fremstillede, heterologe, disulfidbroer indeholdende eukaryotiske proteiner efter ekspression i prokaryotiske celler

Country Status (26)

Country Link
EP (3) EP0219874B1 (es)
JP (2) JPH0728745B2 (es)
KR (1) KR900009139B1 (es)
AT (2) ATE131489T1 (es)
AU (2) AU590029B2 (es)
CA (1) CA1329157C (es)
CZ (1) CZ280727B6 (es)
DD (1) DD260517A5 (es)
DE (3) DE3537708A1 (es)
DK (2) DK175091B1 (es)
ES (2) ES2020498T3 (es)
FI (2) FI94050C (es)
GR (2) GR920300062T1 (es)
HK (2) HK153496A (es)
HR (1) HRP921075B1 (es)
HU (2) HUT43643A (es)
IE (1) IE62634B1 (es)
IL (1) IL80325A (es)
PT (1) PT83609B (es)
SI (1) SI8611796B (es)
SK (1) SK278317B6 (es)
SU (1) SU1607689A3 (es)
UA (1) UA6023A1 (es)
WO (1) WO1987002673A2 (es)
YU (1) YU47185B (es)
ZA (1) ZA868012B (es)

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JP2581668B2 (ja) * 1985-11-27 1997-02-12 三井東圧化学株式会社 ヒト正常細胞由来のヒト組織プラスミノ−ゲン活性化因子をコ−ドする新しいｄｎａ配列とそれを含むベクタ−及び細胞
US4777043A (en) * 1985-12-17 1988-10-11 Genentech, Inc. Stabilized human tissue plasminogen activator compositions
AU621051B2 (en) 1987-04-28 1992-03-05 Amgen, Inc. Method for purifying granulocyte-macrophage colony stimulating factor
DE3722082A1 (de) * 1987-07-03 1989-01-12 Behringwerke Ag Verfahren zur bestimmung der aktivitaet von serinproteasen oder serinproteaseinhibitoren
CA1340586C (en) * 1988-09-23 1999-06-08 Cetus Corporation Process for recovering microbially produced interferon-beta
DE3832898A1 (de) * 1988-09-28 1990-04-12 Boehringer Mannheim Gmbh Praeparat von in prokaryonten exprimiertem plasminogenaktivator
DE3835350A1 (de) * 1988-10-17 1990-04-19 Boehringer Mannheim Gmbh Aktivierung von gentechnologisch hergestellten, in prokaryonten exprimierten antikoerpern
DE3903581A1 (de) * 1989-02-07 1990-08-16 Boehringer Mannheim Gmbh Gewebs-plasminogenaktivator-derivat
DE3942143A1 (de) * 1989-12-20 1991-06-27 Boehringer Mannheim Gmbh T-pa pro stabilisierung
ATE154073T1 (de) * 1990-08-20 1997-06-15 Novo Nordisk As Prozess für die herstellung von biologisch aktivem igf-1 durch verwendung von amino-terminal verlängertem igf-1
DE69129747T2 (de) * 1990-09-05 1998-11-12 Southern Cross Biotech Pty Ltd In lösung bringen von proteinen in aktiver form
DE4037196A1 (de) * 1990-11-22 1992-05-27 Boehringer Mannheim Gmbh Verfahren zur reaktivierung von denaturiertem protein
DE4113750A1 (de) 1991-04-26 1992-10-29 Boehringer Mannheim Gmbh Verbesserung der renaturierung bei der sekretion von disulfidverbrueckten proteinen
DE4139000A1 (de) 1991-11-27 1993-06-03 Boehringer Mannheim Gmbh Verfahren zur gentechnologischen herstellung von biologisch aktivem ss-ngf
US5212091A (en) * 1992-03-02 1993-05-18 Monsanto Company Method of producing tissue factor pathway inhibitor
WO1993019084A1 (en) * 1992-03-24 1993-09-30 Synergen, Inc. Refolding and purification of insulin-like growth factor i
EP0600372B1 (de) 1992-12-02 1997-02-05 Hoechst Aktiengesellschaft Verfahren zur Gewinnung von Proinsulin mit korrekt verbundenen Cystinbrücken
DE4405179A1 (de) * 1994-02-18 1995-08-24 Hoechst Ag Verfahren zur Gewinnung von Insulin mit korrekt verbundenen Cystinbrücken
FR2729972B1 (fr) * 1995-01-31 1997-04-18 Sanofi Sa Procede d'extraction de proteines periplasmiques de microorganismes procaryotes en presence d'arginine
US5714371A (en) * 1995-05-12 1998-02-03 Schering Corporation Method for refolding insoluble aggregates of hepatitis C virus protease
US5728804A (en) * 1995-06-02 1998-03-17 Research Corporation Technologies, Inc. Use of cyclodextrins for protein renaturation
CA2257122C (en) * 1996-06-11 2004-04-06 Boehringer Mannheim Gmbh Method of activating denatured protein
US7153943B2 (en) 1997-07-14 2006-12-26 Bolder Biotechnology, Inc. Derivatives of growth hormone and related proteins, and methods of use thereof
US6653098B1 (en) * 1998-02-23 2003-11-25 G. D. Searle & Co. Method of producing mouse and human endostatin
DE19850429A1 (de) * 1998-10-27 2000-05-04 Andre Schrattenholz Fragmente
EP1048732A1 (de) * 1999-04-26 2000-11-02 F. Hoffmann-La Roche Ag Verfahren zur Herstellung von natürlich gefalteten und sekretierten Proteinen
EP1077263A1 (de) * 1999-07-29 2001-02-21 F.Hoffmann-La Roche Ag Verfahren zur Herstellung von natürlich gefalteten und sekretierten Proteinen durch Co-Sekretion von Chaperonen
DE60129432T2 (de) 2000-05-16 2008-04-17 Bolder Biotechnology, Inc., Louisville Verfahren zur rückfaltung von proteinen mit freien cysteinresten
DE10105912A1 (de) * 2001-02-09 2002-08-14 Roche Diagnostics Gmbh Rekombinante Proteinase K
DE10105911A1 (de) 2001-02-09 2002-08-14 Roche Diagnostics Gmbh Expression der rekombinanten Proteinase K aus Tritirachium album in Hefe
DE102005033250A1 (de) 2005-07-15 2007-01-18 Bioceuticals Arzneimittel Ag Verfahren zur Reinigung von G-CSF
DE202006020194U1 (de) 2006-03-01 2007-12-06 Bioceuticals Arzneimittel Ag G-CSF-Flüssigformulierung
WO2008008975A2 (en) 2006-07-14 2008-01-17 Genentech, Inc. Refolding of recombinant proteins
EP2102355B1 (en) 2006-12-14 2016-03-02 Bolder Biotechnology, Inc. Long acting proteins and peptides and methods of making and using the same
AU2011229491B2 (en) 2010-03-17 2015-02-05 Ratiopharm Gmbh Method for obtaining biologically active recombinant human G-CSF
WO2012054679A1 (en) * 2010-10-20 2012-04-26 Medimmune, Llc Methods for processing inclusion bodies
HUP1200171A1 (hu) 2012-03-19 2013-09-30 Richter Gedeon Nyrt Módszerek polipeptidek elõállítására
HUP1200172A2 (en) 2012-03-19 2013-10-28 Richter Gedeon Nyrt Methods for refolding g-csf from inclusion bodies
CN103852527B (zh) * 2012-12-05 2015-05-13 中国科学院大连化学物理研究所 一种高通量蛋白质样品预处理装置
US10457716B2 (en) 2014-08-06 2019-10-29 University Of Notre Dame Du Lac Protein folding and methods of using same
NZ746109A (en) 2016-12-30 2022-12-23 Biogend Therapeutics Co Ltd Recombinant polypeptides and nucleic acid molecules, compositions, and methods of making and uses thereof
WO2022129460A1 (en) 2020-12-18 2022-06-23 Richter Gedeon Nyrt. Methods for the purification of refolded fc-peptide fusion protein

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Also Published As

Publication number Publication date
HUT43643A (en) 1987-11-30
HK153596A (en) 1996-08-16
DD260517A5 (de) 1988-09-28
EP0219874A2 (de) 1987-04-29
ES2061434T3 (es) 1994-12-16
UA6023A1 (uk) 1994-12-29
EP0253823A1 (de) 1988-01-27
WO1987002673A2 (en) 1987-05-07
ZA868012B (en) 1987-06-24
ATE98648T1 (de) 1994-01-15
CA1329157C (en) 1994-05-03
FI933868A0 (fi) 1993-09-03
IE62634B1 (en) 1995-02-22
AU4132189A (en) 1990-01-04
IL80325A (en) 1992-06-21
IL80325A0 (en) 1987-01-30
FI94050B (fi) 1995-03-31
FI95578B (fi) 1995-11-15
FI95578C (fi) 1996-02-26
DK320387D0 (da) 1987-06-23
ES2020498A4 (es) 1991-08-16
YU179686A (en) 1988-06-30
FI872753A0 (fi) 1987-06-22
FI872753A (fi) 1987-06-22
CZ752686A3 (en) 1996-01-17
DE3537708C2 (es) 1993-07-08
DK200001897A (da) 2000-12-18
PT83609A (de) 1986-11-01
ES2020498T3 (es) 1996-04-01
JPS62502895A (ja) 1987-11-19
AU590029B2 (en) 1989-10-26
HRP921075A2 (en) 1995-06-30
HK153496A (en) 1996-08-16
KR900009139B1 (ko) 1990-12-22
DK175109B1 (da) 2004-06-07
YU47185B (sh) 1995-01-31
SU1607689A3 (ru) 1990-11-15
DE3650449D1 (de) 1996-01-25
EP0219874A3 (en) 1988-02-10
GR920300062T1 (en) 1992-08-31
AU6599386A (en) 1987-05-19
DE3537708A1 (de) 1987-04-23
JPH04218387A (ja) 1992-08-07
PT83609B (pt) 1988-10-14
IE862683L (en) 1987-04-23
SK278317B6 (en) 1996-10-02
GR3018410T3 (en) 1996-03-31
WO1987002673A3 (fr) 1987-10-22
EP0393725A1 (de) 1990-10-24
JPH0824594B2 (ja) 1996-03-13
EP0393725B1 (de) 1995-12-13
ATE131489T1 (de) 1995-12-15
HRP921075B1 (en) 1999-02-28
SK752686A3 (en) 1996-10-01
FI933868A (fi) 1993-09-03
EP0219874B1 (de) 1993-12-15
AU607083B2 (en) 1991-02-21
DE3689404D1 (de) 1994-01-27
KR870700601A (ko) 1987-12-30
SI8611796B (sl) 1998-06-30
FI94050C (fi) 1995-07-10
JPH0728745B2 (ja) 1995-04-05
DK320387A (da) 1987-06-23
HU204855B (en) 1992-02-28
CZ280727B6 (cs) 1996-04-17
SI8611796A (sl) 1996-10-31

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