DK1218542T3 - Fremgangsmåder og sammensætninger til lineær isotermisk amplifikation af polynukleotidsekvenser - Google Patents

Fremgangsmåder og sammensætninger til lineær isotermisk amplifikation af polynukleotidsekvenser

Info

Publication number
DK1218542T3
DK1218542T3 DK00963419T DK00963419T DK1218542T3 DK 1218542 T3 DK1218542 T3 DK 1218542T3 DK 00963419 T DK00963419 T DK 00963419T DK 00963419 T DK00963419 T DK 00963419T DK 1218542 T3 DK1218542 T3 DK 1218542T3
Authority
DK
Denmark
Prior art keywords
methods
primer
composite
compositions
polynucleotide sequences
Prior art date
Application number
DK00963419T
Other languages
Danish (da)
English (en)
Inventor
Nurith Kurn
Original Assignee
Nugen Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nugen Technologies Inc filed Critical Nugen Technologies Inc
Application granted granted Critical
Publication of DK1218542T3 publication Critical patent/DK1218542T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Amplifiers (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
DK00963419T 1999-09-13 2000-09-13 Fremgangsmåder og sammensætninger til lineær isotermisk amplifikation af polynukleotidsekvenser DK1218542T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US15360499P 1999-09-13 1999-09-13
US17578000P 2000-01-12 2000-01-12

Publications (1)

Publication Number Publication Date
DK1218542T3 true DK1218542T3 (da) 2004-08-02

Family

ID=26850696

Family Applications (2)

Application Number Title Priority Date Filing Date
DK00963419T DK1218542T3 (da) 1999-09-13 2000-09-13 Fremgangsmåder og sammensætninger til lineær isotermisk amplifikation af polynukleotidsekvenser
DK04002084.4T DK1431303T3 (en) 1999-09-13 2000-09-13 A composition for isothermal linear amplification of polynucleotide sequences

Family Applications After (1)

Application Number Title Priority Date Filing Date
DK04002084.4T DK1431303T3 (en) 1999-09-13 2000-09-13 A composition for isothermal linear amplification of polynucleotide sequences

Country Status (19)

Country Link
US (2) US6251639B1 (ja)
EP (2) EP1431303B1 (ja)
JP (2) JP3929775B2 (ja)
KR (1) KR100527265B1 (ja)
CN (2) CN102586228A (ja)
AT (1) ATE262595T1 (ja)
AU (1) AU783873B2 (ja)
BR (1) BR0014182A (ja)
CA (1) CA2384838C (ja)
DE (1) DE60009323T2 (ja)
DK (2) DK1218542T3 (ja)
ES (2) ES2214319T3 (ja)
HK (1) HK1046021B (ja)
IL (1) IL148091A0 (ja)
MX (1) MXPA02002656A (ja)
NO (1) NO20021223L (ja)
NZ (1) NZ517121A (ja)
PT (1) PT1218542E (ja)
WO (1) WO2001020035A2 (ja)

Families Citing this family (320)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030027126A1 (en) 1997-03-14 2003-02-06 Walt David R. Methods for detecting target analytes and enzymatic reactions
US7622294B2 (en) * 1997-03-14 2009-11-24 Trustees Of Tufts College Methods for detecting target analytes and enzymatic reactions
US6327410B1 (en) 1997-03-14 2001-12-04 The Trustees Of Tufts College Target analyte sensors utilizing Microspheres
US7348181B2 (en) 1997-10-06 2008-03-25 Trustees Of Tufts College Self-encoding sensor with microspheres
US7115884B1 (en) 1997-10-06 2006-10-03 Trustees Of Tufts College Self-encoding fiber optic sensor
JP3662850B2 (ja) 1998-06-24 2005-06-22 イルミナ インコーポレイテッド 微小球を有するアレイセンサーのデコード
US6951722B2 (en) * 1999-03-19 2005-10-04 Takara Bio Inc. Method for amplifying nucleic acid sequence
US6355431B1 (en) 1999-04-20 2002-03-12 Illumina, Inc. Detection of nucleic acid amplification reactions using bead arrays
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
CA2374390A1 (en) * 1999-05-20 2000-12-14 Illumina, Inc. Combinatorial decoding of random nucleic acid arrays
US6544732B1 (en) 1999-05-20 2003-04-08 Illumina, Inc. Encoding and decoding of array sensors utilizing nanocrystals
US8080380B2 (en) * 1999-05-21 2011-12-20 Illumina, Inc. Use of microfluidic systems in the detection of target analytes using microsphere arrays
US8481268B2 (en) 1999-05-21 2013-07-09 Illumina, Inc. Use of microfluidic systems in the detection of target analytes using microsphere arrays
US7604996B1 (en) 1999-08-18 2009-10-20 Illumina, Inc. Compositions and methods for preparing oligonucleotide solutions
US6692918B2 (en) 1999-09-13 2004-02-17 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences
US7611869B2 (en) * 2000-02-07 2009-11-03 Illumina, Inc. Multiplexed methylation detection methods
US8076063B2 (en) 2000-02-07 2011-12-13 Illumina, Inc. Multiplexed methylation detection methods
US7955794B2 (en) * 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
US7361488B2 (en) * 2000-02-07 2008-04-22 Illumina, Inc. Nucleic acid detection methods using universal priming
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
ATE492652T1 (de) * 2000-02-07 2011-01-15 Illumina Inc Nukleinsäuredetektionsverfahren mit universellem priming
WO2001057268A2 (en) 2000-02-07 2001-08-09 Illumina, Inc. Nucleic acid detection methods using universal priming
US6913884B2 (en) * 2001-08-16 2005-07-05 Illumina, Inc. Compositions and methods for repetitive use of genomic DNA
JP4954415B2 (ja) * 2000-02-10 2012-06-13 イルミナ インコーポレイテッド 試料のビーズをベースとした同時プロセシングのための基材としての個々のアレイのアレイおよびその製造法
US6770441B2 (en) 2000-02-10 2004-08-03 Illumina, Inc. Array compositions and methods of making same
US7285384B2 (en) 2000-02-16 2007-10-23 Illuminia, Inc. Parallel genotyping of multiple patient samples
CN1498274A (zh) 2000-06-26 2004-05-19 Ŧب������˾ 基于转录的核酸扩增方法和组合物
US7846733B2 (en) * 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
EP1322656B1 (en) * 2000-09-26 2008-01-16 Idera Pharmaceuticals, Inc. Modulation of immunostimulatory activity of immunostimulatory oligonucleotide analogs by positional chemical changes
WO2002029117A2 (en) 2000-10-06 2002-04-11 Nugen Technologies, Inc. Methods and probes for detection and/or quantification of nucleic acid sequences
US20040018491A1 (en) * 2000-10-26 2004-01-29 Kevin Gunderson Detection of nucleic acid reactions on bead arrays
EP1366191A2 (en) 2000-12-11 2003-12-03 Alexion Pharmaceuticals, Inc. Nested oligonucleotides containing hairpin for nucleic acid amplification
CA2430329A1 (en) 2000-12-13 2002-06-20 Nugen Technologies, Inc. Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
EP1359219A4 (en) * 2001-02-06 2004-09-22 Takara Bio Inc AMPLIFIED NUCLEIC ACIDS AND IMMOBILIZED PRODUCTS THEREOF
JP2005508135A (ja) * 2001-03-09 2005-03-31 ニューゲン テクノロジーズ， インコーポレイテッド Ｒｎａ配列の増幅のための方法および組成物
US6946251B2 (en) 2001-03-09 2005-09-20 Nugen Technologies, Inc. Methods and compositions for amplification of RNA sequences using RNA-DNA composite primers
US8137911B2 (en) 2001-05-22 2012-03-20 Cellscript, Inc. Preparation and use of single-stranded transcription substrates for synthesis of transcription products corresponding to target sequences
US7250552B2 (en) 2001-06-07 2007-07-31 Pioneer Hi-Bred International, Inc. QTL controlling sclerotinia stem rot resistance in soybean
AU2002311183B2 (en) * 2001-06-12 2007-03-01 Takara Bio Inc. Method of stabilizing reagent for amplifying or detecting nucleic acid and storage method
US9777312B2 (en) * 2001-06-30 2017-10-03 Enzo Life Sciences, Inc. Dual polarity analysis of nucleic acids
US9261460B2 (en) 2002-03-12 2016-02-16 Enzo Life Sciences, Inc. Real-time nucleic acid detection processes and compositions
US20040161741A1 (en) 2001-06-30 2004-08-19 Elazar Rabani Novel compositions and processes for analyte detection, quantification and amplification
EP1481076A4 (en) 2001-07-12 2005-05-11 Illumina Inc MULTIPLEX NUCLEIC REACTIONS
TWI257426B (en) * 2001-08-20 2006-07-01 Takara Bio Inc Nucleic acid amplification methods
WO2005110493A2 (en) * 2004-04-16 2005-11-24 Bioexpertise Llc Methods for delivering mbd peptide-linked agent into cells under conditions of cellular stress
JP4439262B2 (ja) * 2001-09-19 2010-03-24 アレクシオン ファーマシューティカルズ， インコーポレイテッド 操作されたテンプレートおよび単一プライマー増幅におけるそれらの使用
US7414111B2 (en) * 2001-09-19 2008-08-19 Alexion Pharmaceuticals, Inc. Engineered templates and their use in single primer amplification
DE10151069A1 (de) 2001-10-05 2003-04-30 Epigenomics Ag Verfahren zum Nachweis von DNA-Methylierung mittels markierten S-Adenosylmethioninanaloga
WO2003069333A1 (en) 2002-02-14 2003-08-21 Illumina, Inc. Automated information processing in randomly ordered arrays
US6977162B2 (en) * 2002-03-01 2005-12-20 Ravgen, Inc. Rapid analysis of variations in a genome
ATE408711T1 (de) * 2002-03-01 2008-10-15 Integrated Dna Tech Inc Polynomiale amplifikation von nukleinsäuren
US7629152B2 (en) * 2002-03-01 2009-12-08 Integrated Dna Technologies, Inc. Methods for amplifying polymeric nucleic acids
KR20040102024A (ko) * 2002-03-01 2004-12-03 라브겐, 인코퍼레이티드 유전적 장애의 검출 방법
WO2003078645A2 (en) * 2002-03-11 2003-09-25 Nugen Technologies, Inc. Methods for generating double stranded dna comprising a 3’ single stranded portion and uses of these complexes for recombination
US9353405B2 (en) 2002-03-12 2016-05-31 Enzo Life Sciences, Inc. Optimized real time nucleic acid detection processes
CA2480525A1 (en) * 2002-03-29 2003-10-09 Nugen Technologies, Inc. Single primer isothermal nucleic acid amplification-enhanced analyte detection and quantification
US7442506B2 (en) * 2002-05-08 2008-10-28 Ravgen, Inc. Methods for detection of genetic disorders
US7727720B2 (en) * 2002-05-08 2010-06-01 Ravgen, Inc. Methods for detection of genetic disorders
US20070178478A1 (en) * 2002-05-08 2007-08-02 Dhallan Ravinder S Methods for detection of genetic disorders
WO2004011665A2 (en) * 2002-05-17 2004-02-05 Nugen Technologies, Inc. Methods for fragmentation, labeling and immobilization of nucleic acids
US7476519B2 (en) * 2002-07-19 2009-01-13 Joseph Monforte Strategies for gene expression analysis
US20040259105A1 (en) * 2002-10-03 2004-12-23 Jian-Bing Fan Multiplex nucleic acid analysis using archived or fixed samples
US20050003369A1 (en) * 2002-10-10 2005-01-06 Affymetrix, Inc. Method for depleting specific nucleic acids from a mixture
AU2003300565A1 (en) 2002-10-11 2004-05-04 Davi, Frederic Bernard, Louis Nucleic acid amplification primers for pcr-based clonality studies
WO2004048594A2 (en) * 2002-11-21 2004-06-10 Epicentre Technologies Preparation and use of single-stranded transcription substrates for synthesis of transcription products corresponding to target sequences
US20040115643A1 (en) * 2002-12-12 2004-06-17 Lizardi Paul M. Thermodynamic equilibrium extension of primers
US9487823B2 (en) * 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
US7887752B2 (en) * 2003-01-21 2011-02-15 Illumina, Inc. Chemical reaction monitor
US20040157219A1 (en) * 2003-02-06 2004-08-12 Jianrong Lou Chemical treatment of biological samples for nucleic acid extraction and kits therefor
CA2897376A1 (en) 2003-02-26 2004-09-10 Radoje T. Drmanac Modular system and probes for dna analysis
WO2004092418A2 (en) 2003-04-14 2004-10-28 Nugen Technologies, Inc. Global amplification using a randomly primed composite primer
EP1618187A4 (en) * 2003-04-29 2006-12-20 Ge Healthcare Bio Sciences AMPLIFICATION OF NUCLEIC ACID SEQUENCES WITH MULTIPLE PRIMING
US7341837B2 (en) 2003-09-02 2008-03-11 Lawton Robert L Soluble analyte detection and amplification
DK1664343T3 (da) 2003-09-09 2014-08-11 Integrigen Inc Fremgangsmåder og sammensætninger til generering af kimcelle humane antistofgener
DE602004026077D1 (de) * 2003-09-10 2010-04-29 Althea Technologies Inc Erstellung von expressionsprofilen unter verwendung von mikroarrays
US20060094064A1 (en) * 2003-11-19 2006-05-04 Sandip Ray Methods and compositions for diagnosis, stratification, and monitoring of alzheimer's disease and other neurological disorders in body fluids
AU2004294178C1 (en) * 2003-11-19 2011-10-27 Rules-Based Medicine, Inc. Methods for diagnosis, stratification, and monitoring of Alzheimer's disease
US7344834B2 (en) * 2003-12-08 2008-03-18 Cytyc Corporation Method for DNA amplification using DNA blocking probes
WO2005065321A2 (en) * 2003-12-29 2005-07-21 Nugen Technologies, Inc. Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids
WO2006033487A1 (en) * 2004-09-21 2006-03-30 Genomictree Inc. Method for linear amplification of rna using high-heel primer
KR100590621B1 (ko) * 2004-09-21 2006-06-19 (주)지노믹트리 하이힐 프라이머를 이용한 ｒｎａ의 선형 증폭방법
US20060240451A1 (en) * 2005-02-09 2006-10-26 Epicentre Technologies Compositions and methods employing 5' phosphate-dependent nucleic acid exonucleases
WO2006087574A2 (en) 2005-02-19 2006-08-24 Geneform Technologies Limited Isothermal nucleic acid amplification
US20060234268A1 (en) * 2005-04-15 2006-10-19 Sungwhan An Linear amplification of rna
KR101762424B1 (ko) 2005-05-09 2017-07-28 테라노스, 인코포레이티드 현장진료 유체 시스템 및 그 용도
EP2477029A1 (en) * 2005-06-02 2012-07-18 Fluidigm Corporation Analysis using microfluidic partitioning devices
US20070020639A1 (en) * 2005-07-20 2007-01-25 Affymetrix, Inc. Isothermal locus specific amplification
EP1929045B9 (en) * 2005-09-06 2012-06-20 Gen-Probe Incorporated Methods, compositions and kits for isothermal amplification of nucleic acids
CA2621267A1 (en) 2005-09-07 2007-03-15 Nugen Technologies, Inc. Improved nucleic acid amplification procedure
US20070065814A1 (en) * 2005-09-21 2007-03-22 Engelhard Eric K Detecting foot-and-mouth disease virus
US7485425B2 (en) * 2005-10-03 2009-02-03 Applied Biosystems Inc. Methods for amplification of nucleic acids using spanning primers
EP2458011B1 (en) 2005-10-03 2013-07-24 Life Technologies Corporation Compositions, methods, and kits for amplifying nucleic acids
US8831887B2 (en) * 2005-10-12 2014-09-09 The Research Foundation For The State University Of New York Absolute PCR quantification
KR100816419B1 (ko) * 2005-10-14 2008-03-27 래플진(주) 핵산의 등온증폭 방법 및 핵산과 신호 프로브의 동시등온증폭을 이용한 핵산의 검출방법
US8609829B2 (en) * 2005-10-17 2013-12-17 Gen-Probe Incorporated Compositions and methods to detect Legionella pneumophila nucleic acid
US7611893B2 (en) 2005-11-09 2009-11-03 Ontherix, Inc. Metal-binding therapeutic peptides
US20080039393A1 (en) * 2005-11-09 2008-02-14 Desmond Mascarenhas Metal-binding therapeutic peptides
US7662624B2 (en) * 2005-11-09 2010-02-16 Ontherix, Inc. Metal-binding therapeutic peptides
WO2007056511A2 (en) * 2005-11-09 2007-05-18 Ontherix, Inc. Metal-binding therapeutic peptides
US8741230B2 (en) 2006-03-24 2014-06-03 Theranos, Inc. Systems and methods of sample processing and fluid control in a fluidic system
US11287421B2 (en) 2006-03-24 2022-03-29 Labrador Diagnostics Llc Systems and methods of sample processing and fluid control in a fluidic system
WO2007127999A2 (en) * 2006-04-28 2007-11-08 Igor Kutyavin Use of products of pcr amplification carrying elements of secondary structure to improve pcr-based nucleic acid detection
EP2021488B1 (en) 2006-04-28 2018-09-12 Igor Kutyavin Use of base-modified deoxynucleoside triphosphates to improve nucleic acid detection
CA2652052A1 (en) * 2006-05-16 2007-11-29 Nugen Technologies, Inc. Nucleic acid separation and purification method based on reversible charge interactions
US11001881B2 (en) 2006-08-24 2021-05-11 California Institute Of Technology Methods for detecting analytes
US7833716B2 (en) 2006-06-06 2010-11-16 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods
US20100022403A1 (en) * 2006-06-30 2010-01-28 Nurith Kurn Methods for fragmentation and labeling of nucleic acids
US7501254B2 (en) * 2006-07-20 2009-03-10 Ghc Technologies, Inc. Methods and compositions for amplification and capture of nucleic acid sequences
US11525156B2 (en) 2006-07-28 2022-12-13 California Institute Of Technology Multiplex Q-PCR arrays
US8048626B2 (en) 2006-07-28 2011-11-01 California Institute Of Technology Multiplex Q-PCR arrays
BRPI0715427B1 (pt) * 2006-07-31 2016-04-12 Wanli Bi método para amplificação de sequência ou sinal de ácidos nucleicos alvo, oligonucleotideo modificado reversível, mistura e conjunto de reação de amplificação de ácido nucleico
US9045522B2 (en) 2006-07-31 2015-06-02 Wanli Bi Nucleic acid amplification using a reversibly modified oligonucleotide
US11560588B2 (en) 2006-08-24 2023-01-24 California Institute Of Technology Multiplex Q-PCR arrays
US20100075305A1 (en) * 2006-10-03 2010-03-25 Gifu University Detection of bacterium by utilizing dnaj gene and use thereof
WO2008058018A2 (en) 2006-11-02 2008-05-15 Mayo Foundation For Medical Education And Research Predicting cancer outcome
US7902345B2 (en) 2006-12-05 2011-03-08 Sequenom, Inc. Detection and quantification of biomolecules using mass spectrometry
EP3095873B1 (en) 2006-12-21 2018-04-18 Gen-Probe Incorporated Methods and compositions for nucleic acid amplification
JP5194459B2 (ja) * 2007-01-25 2013-05-08 ソニー株式会社 一本鎖ｄｎａ増幅方法
EP2174130A2 (en) 2007-06-21 2010-04-14 Momenta Pharmaceuticals, Inc. Copolymer assay
ES2647237T3 (es) * 2007-07-03 2017-12-20 Genaphora Ltd. Cebadores quiméricos para reacciones de amplificación de ácidos nucleicos mejoradas
WO2009042291A2 (en) * 2007-08-03 2009-04-02 Igor Kutyavin Accelerated cascade amplification (aca) of nucleic acids comprising strand and sequence specific dna nicking
EP2195452B1 (en) 2007-08-29 2012-03-14 Sequenom, Inc. Methods and compositions for universal size-specific polymerase chain reaction
US8716190B2 (en) 2007-09-14 2014-05-06 Affymetrix, Inc. Amplification and analysis of selected targets on solid supports
US9388457B2 (en) 2007-09-14 2016-07-12 Affymetrix, Inc. Locus specific amplification using array probes
EP2657699B1 (en) 2007-10-02 2017-03-22 Theranos, Inc. Modular point-of-care devices and uses thereof
KR100957057B1 (ko) 2007-12-03 2010-05-13 래플진(주) 핵산과 신호 프로브의 동시 등온증폭을 이용한 핵산의검출방법
US20090162845A1 (en) 2007-12-20 2009-06-25 Elazar Rabbani Affinity tag nucleic acid and protein compositions, and processes for using same
US20090203531A1 (en) * 2008-02-12 2009-08-13 Nurith Kurn Method for Archiving and Clonal Expansion
US20090215050A1 (en) * 2008-02-22 2009-08-27 Robert Delmar Jenison Systems and methods for point-of-care amplification and detection of polynucleotides
US8536135B2 (en) * 2008-03-19 2013-09-17 Ontherix, Inc. Adaptive biochemical signatures
US7846666B2 (en) 2008-03-21 2010-12-07 Nugen Technologies, Inc. Methods of RNA amplification in the presence of DNA
US8911948B2 (en) * 2008-04-30 2014-12-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
EP2279263B1 (en) 2008-04-30 2013-09-04 Integrated Dna Technologies, Inc. Rnase-h-based assays utilizing modified rna monomers
WO2009137369A1 (en) * 2008-05-03 2009-11-12 Tufts Medical Center, Inc. Neonatal salivary genomics
AU2009253675A1 (en) 2008-05-28 2009-12-03 Genomedx Biosciences, Inc. Systems and methods for expression-based discrimination of distinct clinical disease states in prostate cancer
US10407731B2 (en) 2008-05-30 2019-09-10 Mayo Foundation For Medical Education And Research Biomarker panels for predicting prostate cancer outcomes
LT2304054T (lt) 2008-06-11 2017-10-10 Orion Pharma (Uk) Limited Izoterminė nukleorūgšties amplifkacija
WO2010030716A1 (en) * 2008-09-10 2010-03-18 Igor Kutyavin Detection of nucleic acids by oligonucleotide probes cleaved in presence of endonuclease v
CA2641132A1 (en) * 2008-10-03 2010-04-03 Richard T. Scott, Jr. Improvements in in vitro fertilization
US20100124756A1 (en) * 2008-10-10 2010-05-20 Sandip Ray Collection of biomarkers for diagnosis and monitoring of alzheimer's disease in body fluids
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
DK2963709T3 (en) 2008-10-24 2017-09-11 Epicentre Tech Corp TRANSPOSON-END COMPOSITIONS AND PROCEDURES FOR MODIFICATION OF NUCLEIC ACIDS
US9495515B1 (en) 2009-12-09 2016-11-15 Veracyte, Inc. Algorithms for disease diagnostics
US10236078B2 (en) 2008-11-17 2019-03-19 Veracyte, Inc. Methods for processing or analyzing a sample of thyroid tissue
US20100206316A1 (en) * 2009-01-21 2010-08-19 Scott Jr Richard T Method for determining chromosomal defects in an ivf embryo
US9074258B2 (en) 2009-03-04 2015-07-07 Genomedx Biosciences Inc. Compositions and methods for classifying thyroid nodule disease
CN102428191A (zh) * 2009-03-18 2012-04-25 塞昆纳姆股份有限公司 热稳定性内切核酸酶在产生报道分子中的应用
EP3360978A3 (en) 2009-05-07 2018-09-26 Veracyte, Inc. Methods for diagnosis of thyroid conditions
EP2432899A1 (en) 2009-05-22 2012-03-28 Population Genetics Technologies LTD. Sorting asymmetrically tagged nucleic acids by selective primer extension
US20100317916A1 (en) * 2009-06-12 2010-12-16 Scott Jr Richard T Method for relative quantitation of chromosomal DNA copy number in single or few cells
WO2011003020A1 (en) 2009-07-01 2011-01-06 Gen-Probe Incorporated Methods and compositions for nucleic acid amplification
US20110189679A1 (en) * 2009-09-11 2011-08-04 Nugen Technologies, Inc. Compositions and methods for whole transcriptome analysis
WO2011037802A2 (en) 2009-09-28 2011-03-31 Igor Kutyavin Methods and compositions for detection of nucleic acids based on stabilized oligonucleotide probe complexes
US20110129832A1 (en) * 2009-10-27 2011-06-02 Swift Biosciences, Inc. Polynucleotide Primers and Probes
US10446272B2 (en) 2009-12-09 2019-10-15 Veracyte, Inc. Methods and compositions for classification of samples
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
CN102725424B (zh) 2010-01-25 2014-07-09 Rd生物科技公司 靶核酸的自折叠扩增
US20110202284A1 (en) * 2010-02-10 2011-08-18 Mcreynolds Cristopher Novel groups of biomarkers for diagnosing alzheimer's disease
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
CN101935697B (zh) * 2010-04-16 2015-11-25 中生方政生物技术有限公司 用于核酸序列检测的方法和试剂盒
TWI748368B (zh) 2011-01-21 2021-12-01 美商拉布拉多診斷有限責任公司 樣本使用最大化之系統及方法
WO2012135053A2 (en) 2011-03-25 2012-10-04 Integrated Dna Technologies, Inc. Rnase h-based assays utilizing modified rna monomers
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
BR112013028296B1 (pt) 2011-05-04 2020-03-24 Biocept, Inc. Métodos para detecção de variantes de sequências de ácido nucléico
US10704164B2 (en) 2011-08-31 2020-07-07 Life Technologies Corporation Methods, systems, computer readable media, and kits for sample identification
US9619627B2 (en) 2011-09-25 2017-04-11 Theranos, Inc. Systems and methods for collecting and transmitting assay results
US9268915B2 (en) 2011-09-25 2016-02-23 Theranos, Inc. Systems and methods for diagnosis or treatment
US8435738B2 (en) 2011-09-25 2013-05-07 Theranos, Inc. Systems and methods for multi-analysis
US8840838B2 (en) 2011-09-25 2014-09-23 Theranos, Inc. Centrifuge configurations
US8475739B2 (en) 2011-09-25 2013-07-02 Theranos, Inc. Systems and methods for fluid handling
US8380541B1 (en) 2011-09-25 2013-02-19 Theranos, Inc. Systems and methods for collecting and transmitting assay results
US9632102B2 (en) 2011-09-25 2017-04-25 Theranos, Inc. Systems and methods for multi-purpose analysis
US9664702B2 (en) 2011-09-25 2017-05-30 Theranos, Inc. Fluid handling apparatus and configurations
US20140170735A1 (en) 2011-09-25 2014-06-19 Elizabeth A. Holmes Systems and methods for multi-analysis
US10012664B2 (en) 2011-09-25 2018-07-03 Theranos Ip Company, Llc Systems and methods for fluid and component handling
US9250229B2 (en) 2011-09-25 2016-02-02 Theranos, Inc. Systems and methods for multi-analysis
US9810704B2 (en) 2013-02-18 2017-11-07 Theranos, Inc. Systems and methods for multi-analysis
BR112014007073A2 (pt) 2011-09-25 2017-04-11 Theranos Inc sistemas e métodos para multi-análise
WO2013059746A1 (en) 2011-10-19 2013-04-25 Nugen Technologies, Inc. Compositions and methods for directional nucleic acid amplification and sequencing
CA2858581A1 (en) 2011-12-13 2013-06-20 Genomedx Biosciences, Inc. Cancer diagnostics using non-coding transcripts
US9422601B2 (en) 2011-12-15 2016-08-23 Samsung Electronics Co., Ltd. Method of determining a ratio of RNA species in a sample
US20130217071A1 (en) 2011-12-30 2013-08-22 Luz Montesclaros Methods and compositions for performing nucleic acid amplification reactions
EP3578697B1 (en) 2012-01-26 2024-03-06 Tecan Genomics, Inc. Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
JP6399660B2 (ja) 2012-04-10 2018-10-03 ザ・リージエンツ・オブ・ザ・ユニバーシテイー・オブ・カリフオルニア 癌治療用組成物および方法
EP2847353B1 (en) 2012-05-10 2022-01-19 The General Hospital Corporation Methods for determining a nucleotide sequence
US9840732B2 (en) 2012-05-21 2017-12-12 Fluidigm Corporation Single-particle analysis of particle populations
WO2013191775A2 (en) 2012-06-18 2013-12-27 Nugen Technologies, Inc. Compositions and methods for negative selection of non-desired nucleic acid sequences
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
US11035005B2 (en) 2012-08-16 2021-06-15 Decipher Biosciences, Inc. Cancer diagnostics using biomarkers
BR112015005429A2 (pt) 2012-09-11 2017-07-04 Theranos Inc sistemas de gestão de informação e métodos usando uma assinatura biológica
EP2912197B1 (en) * 2012-10-24 2019-08-07 Takara Bio USA, Inc. Template switch-based methods for producing a product nucleic acid
AU2013352067A1 (en) 2012-11-28 2015-06-04 Abwiz Bio, Inc. Preparation of gene-specific templates for the use in single primer amplification
US20150275285A1 (en) * 2012-12-03 2015-10-01 Yilin Zhang Compositions and methods of nucleic acid preparation and analyses
US20150299772A1 (en) * 2012-12-03 2015-10-22 Elim Biopharmaceuticals, Inc. Single-stranded polynucleotide amplification methods
SG11201506421PA (en) 2013-02-18 2015-09-29 Theranos Inc Systems and methods for collecting and transmitting assay results
WO2014144092A1 (en) 2013-03-15 2014-09-18 Nugen Technologies, Inc. Sequential sequencing
US9227978B2 (en) 2013-03-15 2016-01-05 Araxes Pharma Llc Covalent inhibitors of Kras G12C
US11976329B2 (en) 2013-03-15 2024-05-07 Veracyte, Inc. Methods and systems for detecting usual interstitial pneumonia
AU2014257553A1 (en) 2013-04-25 2015-11-12 Orion Diagnostica Oy Strand-invasion based DNA amplification method
DK3013983T3 (da) 2013-06-25 2023-03-06 Prognosys Biosciences Inc Spatialt kodede biologiske assays ved brug af en mikrofluidisk anordning
SG11201600550WA (en) 2013-07-25 2016-02-26 Dch Molecular Diagnostics Inc Methods and compositions for detecting bacterial contamination
WO2015021460A1 (en) * 2013-08-09 2015-02-12 Luminex Corporation Probes for improved melt discrimination and multiplexing in nucleic acid assays
US9410172B2 (en) * 2013-09-16 2016-08-09 General Electric Company Isothermal amplification using oligocation-conjugated primer sequences
TWI659021B (zh) 2013-10-10 2019-05-11 亞瑞克西斯製藥公司 Ｋｒａｓ ｇ１２ｃ之抑制劑
US10941397B2 (en) 2013-10-17 2021-03-09 Takara Bio Usa, Inc. Methods for adding adapters to nucleic acids and compositions for practicing the same
EP3068883B1 (en) 2013-11-13 2020-04-29 Nugen Technologies, Inc. Compositions and methods for identification of a duplicate sequencing read
EP3628747B1 (en) 2013-12-05 2022-10-05 Centrillion Technology Holdings Corporation Fabrication of patterned arrays
US10767222B2 (en) 2013-12-11 2020-09-08 Accuragen Holdings Limited Compositions and methods for detecting rare sequence variants
US11286519B2 (en) 2013-12-11 2022-03-29 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
US11859246B2 (en) 2013-12-11 2024-01-02 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
US9719136B2 (en) 2013-12-17 2017-08-01 Takara Bio Usa, Inc. Methods for adding adapters to nucleic acids and compositions for practicing the same
US10450597B2 (en) 2014-01-27 2019-10-22 The General Hospital Corporation Methods of preparing nucleic acids for sequencing
WO2015121236A1 (en) * 2014-02-11 2015-08-20 F. Hoffmann-La Roche Ag Targeted sequencing and uid filtering
WO2015131107A1 (en) 2014-02-28 2015-09-03 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
GB201410022D0 (en) 2014-06-05 2014-07-16 Orion Diagnostica Oy Method
US10017759B2 (en) 2014-06-26 2018-07-10 Illumina, Inc. Library preparation of tagged nucleic acid
SG11201700891SA (en) 2014-08-06 2017-03-30 Nugen Technologies Inc Digital measurements from targeted sequencing
JO3556B1 (ar) 2014-09-18 2020-07-05 Araxes Pharma Llc علاجات مدمجة لمعالجة السرطان
WO2016049568A1 (en) 2014-09-25 2016-03-31 Araxes Pharma Llc Methods and compositions for inhibition of ras
JP2017528498A (ja) 2014-09-25 2017-09-28 アラクセス ファーマ エルエルシー Ｋｒａｓ ｇ１２ｃ変異体タンパク質のインヒビター
CN114606309A (zh) 2014-11-05 2022-06-10 威拉赛特公司 使用机器学习和高维转录数据的诊断系统和方法
CN114438174A (zh) 2014-11-11 2022-05-06 伊鲁米那股份有限公司 使用crispr-cas系统的多核苷酸扩增
US10760182B2 (en) * 2014-12-16 2020-09-01 Apdn (B.V.I.) Inc. Method and device for marking fibrous materials
US9708647B2 (en) 2015-03-23 2017-07-18 Insilixa, Inc. Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays
CN107532207B (zh) 2015-04-10 2021-05-07 空间转录公司 生物样本的空间区别、多重核酸分析
US10246424B2 (en) 2015-04-10 2019-04-02 Araxes Pharma Llc Substituted quinazoline compounds and methods of use thereof
ES2856880T3 (es) 2015-04-15 2021-09-28 Araxes Pharma Llc Inhibidores tricíclicos condensados de KRAS y métodos de uso de los mismos
EP3295345B1 (en) 2015-05-14 2023-01-25 Life Technologies Corporation Barcode sequences, and related systems and methods
EP3103885B1 (en) 2015-06-09 2019-01-30 Centrillion Technology Holdings Corporation Methods for sequencing nucleic acids
US10144724B2 (en) 2015-07-22 2018-12-04 Araxes Pharma Llc Substituted quinazoline compounds and methods of use thereof
US9499861B1 (en) 2015-09-10 2016-11-22 Insilixa, Inc. Methods and systems for multiplex quantitative nucleic acid amplification
US20180360959A1 (en) 2015-09-25 2018-12-20 Universität Duisburg-Essen Agents inhibiting kallikrein-8 for use in the prevention or treatment of alzheimer's disease
US10858343B2 (en) 2015-09-28 2020-12-08 Araxes Pharma Llc Inhibitors of KRAS G12C mutant proteins
EP3356359B1 (en) 2015-09-28 2021-10-20 Araxes Pharma LLC Inhibitors of kras g12c mutant proteins
US10875842B2 (en) 2015-09-28 2020-12-29 Araxes Pharma Llc Inhibitors of KRAS G12C mutant proteins
US10647703B2 (en) 2015-09-28 2020-05-12 Araxes Pharma Llc Inhibitors of KRAS G12C mutant proteins
US10730867B2 (en) 2015-09-28 2020-08-04 Araxes Pharma Llc Inhibitors of KRAS G12C mutant proteins
EP3356349A1 (en) 2015-09-28 2018-08-08 Araxes Pharma LLC Inhibitors of kras g12c mutant proteins
US10689356B2 (en) 2015-09-28 2020-06-23 Araxes Pharma Llc Inhibitors of KRAS G12C mutant proteins
SG11201802864RA (en) 2015-10-09 2018-05-30 Accuragen Holdings Ltd Methods and compositions for enrichment of amplification products
EP3364977A4 (en) 2015-10-19 2019-09-04 Araxes Pharma LLC PROCESS FOR SCREENING INHIBITORS OF RAS
US10414757B2 (en) 2015-11-16 2019-09-17 Araxes Pharma Llc 2-substituted quinazoline compounds comprising a substituted heterocyclic group and methods of use thereof
US20180363039A1 (en) 2015-12-03 2018-12-20 Accuragen Holdings Limited Methods and compositions for forming ligation products
US9988357B2 (en) 2015-12-09 2018-06-05 Araxes Pharma Llc Methods for preparation of quinazoline derivatives
WO2017155858A1 (en) 2016-03-07 2017-09-14 Insilixa, Inc. Nucleic acid sequence identification using solid-phase cyclic single base extension
US10822312B2 (en) 2016-03-30 2020-11-03 Araxes Pharma Llc Substituted quinazoline compounds and methods of use
US10619205B2 (en) 2016-05-06 2020-04-14 Life Technologies Corporation Combinatorial barcode sequences, and related systems and methods
EP4269611A3 (en) 2016-05-11 2024-01-17 Illumina, Inc. Polynucleotide enrichment and amplification using argonaute systems
CN109511265B (zh) 2016-05-16 2023-07-14 安可济控股有限公司 通过链鉴定改进测序的方法
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10144962B2 (en) 2016-06-30 2018-12-04 Grail, Inc. Differential tagging of RNA for preparation of a cell-free DNA/RNA sequencing library
US10646488B2 (en) 2016-07-13 2020-05-12 Araxes Pharma Llc Conjugates of cereblon binding compounds and G12C mutant KRAS, HRAS or NRAS protein modulating compounds and methods of use thereof
WO2018035170A1 (en) 2016-08-15 2018-02-22 Accuragen Holdings Limited Compositions and methods for detecting rare sequence variants
EP3504348B1 (en) 2016-08-24 2022-12-14 Decipher Biosciences, Inc. Use of genomic signatures to predict responsiveness of patients with prostate cancer to post-operative radiation therapy
CA3037190A1 (en) 2016-09-15 2018-03-22 ArcherDX, Inc. Methods of nucleic acid sample preparation for analysis of cell-free dna
CN109937254B (zh) 2016-09-15 2023-05-30 阿谢尔德克斯有限责任公司 核酸样品制备方法
EP3519402A1 (en) 2016-09-29 2019-08-07 Araxes Pharma LLC Inhibitors of kras g12c mutant proteins
EP3736324A1 (en) 2016-09-30 2020-11-11 The Governing Council Of The University Of Toronto System for identifying and targeting individual cells within a heterogeneous population for selective extraction of cellular content
US10377743B2 (en) 2016-10-07 2019-08-13 Araxes Pharma Llc Inhibitors of RAS and methods of use thereof
CN106498040B (zh) * 2016-10-12 2018-10-23 浙江大学 一种分子标记微珠及基于该分子标记微珠的高通量单细胞测序方法
US10190155B2 (en) 2016-10-14 2019-01-29 Nugen Technologies, Inc. Molecular tag attachment and transfer
JP7191828B2 (ja) 2016-12-15 2022-12-19 ザ・リージエンツ・オブ・ザ・ユニバーシテイー・オブ・カリフオルニア がん治療のための組成物および方法
GB201621477D0 (en) * 2016-12-16 2017-02-01 Multiplicom Nv Modified multiplex and multistep amplification reactions and reagents therefor
EP4095263A1 (en) 2017-01-06 2022-11-30 Editas Medicine, Inc. Methods of assessing nuclease cleavage
US11208697B2 (en) 2017-01-20 2021-12-28 Decipher Biosciences, Inc. Molecular subtyping, prognosis, and treatment of bladder cancer
EP3573971A1 (en) 2017-01-26 2019-12-04 Araxes Pharma LLC 1-(3-(6-(3-hydroxynaphthalen-1-yl)benzofuran-2-yl)azetidin-1yl)prop-2-en-1-one derivatives and similar compounds as kras g12c modulators for treating cancer
EP3573964A1 (en) 2017-01-26 2019-12-04 Araxes Pharma LLC Benzothiophene and benzothiazole compounds and methods of use thereof
EP3573967A1 (en) 2017-01-26 2019-12-04 Araxes Pharma LLC Fused hetero-hetero bicyclic compounds and methods of use thereof
WO2018140512A1 (en) 2017-01-26 2018-08-02 Araxes Pharma Llc Fused bicyclic benzoheteroaromatic compounds and methods of use thereof
EP3573970A1 (en) 2017-01-26 2019-12-04 Araxes Pharma LLC 1-(6-(3-hydroxynaphthalen-1-yl)quinazolin-2-yl)azetidin-1-yl)prop-2-en-1-one derivatives and similar compounds as kras g12c inhibitors for the treatment of cancer
EP3593140A4 (en) 2017-03-09 2021-01-06 Decipher Biosciences, Inc. SUBTYPING PROSTATE CANCER TO PREDICT RESPONSE TO HORMONE THERAPY
AU2018266733A1 (en) 2017-05-12 2020-01-16 Veracyte, Inc. Genetic signatures to predict prostate cancer metastasis and identify tumor aggressiveness
JP7056012B2 (ja) * 2017-05-19 2022-04-19 トヨタ自動車株式会社 ランダムプライマーセット、及びこれを用いたｄｎａライブラリーの作製方法
US10914729B2 (en) 2017-05-22 2021-02-09 The Trustees Of Princeton University Methods for detecting protein binding sequences and tagging nucleic acids
CA3063440A1 (en) 2017-05-25 2018-11-29 Araxes Pharma Llc Covalent inhibitors of kras
WO2018218069A1 (en) 2017-05-25 2018-11-29 Araxes Pharma Llc Quinazoline derivatives as modulators of mutant kras, hras or nras
JP2020521741A (ja) 2017-05-25 2020-07-27 アラクセス ファーマ エルエルシー がんの処置のための化合物およびその使用の方法
US11217329B1 (en) 2017-06-23 2022-01-04 Veracyte, Inc. Methods and systems for determining biological sample integrity
US11667952B2 (en) 2017-08-24 2023-06-06 Takara Bio Usa, Inc. Methods of producing nucleic acids using oligonucleotides modified by a stimulus
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
US10689629B1 (en) 2017-12-06 2020-06-23 Cepheid Inhibition of nucleic acid polymerases by endonuclease V-cleavable circular oligonucleotide ligands
US10724017B1 (en) 2017-12-06 2020-07-28 Cepheid Inhibition of DNA polymerases by uracil-DNA glycosylase-cleavable oligonucleotide ligands
US10724083B1 (en) 2017-12-06 2020-07-28 Cepheid Inhibition of nucleic acid polymerases by endonuclease V-cleavable oligonucleotide ligands
CA3089756A1 (en) * 2018-01-29 2019-08-01 St. Jude Children's Research Hospital, Inc. Method for nucleic acid amplification
US11203782B2 (en) 2018-03-29 2021-12-21 Accuragen Holdings Limited Compositions and methods comprising asymmetric barcoding
US20210292750A1 (en) * 2018-07-24 2021-09-23 Ripple Biosolutions Llc Methods and composition for targeted genomic analysis
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
WO2020123319A2 (en) 2018-12-10 2020-06-18 10X Genomics, Inc. Methods of using master / copy arrays for spatial detection
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
EP3937780A4 (en) 2019-03-14 2022-12-07 InSilixa, Inc. METHODS AND SYSTEMS FOR TIMED FLUORESCENCE-BASED DETECTION
WO2020243579A1 (en) 2019-05-30 2020-12-03 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
WO2021092433A2 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Enhancing specificity of analyte binding
EP4219754B1 (en) 2019-12-23 2024-05-15 10X Genomics, Inc. Methods for spatial analysis using rna-templated ligation
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
ES2965354T3 (es) 2020-04-22 2024-04-12 10X Genomics Inc Métodos para análisis espacial que usan eliminación de ARN elegido como diana
WO2021236929A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
WO2021237087A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Spatial analysis to detect sequence variants
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
EP4162074B1 (en) 2020-06-08 2024-04-24 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
WO2021252591A1 (en) 2020-06-10 2021-12-16 10X Genomics, Inc. Methods for determining a location of an analyte in a biological sample
AU2021294334A1 (en) 2020-06-25 2023-02-02 10X Genomics, Inc. Spatial analysis of DNA methylation
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
AU2021409136A1 (en) 2020-12-21 2023-06-29 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
WO2022192191A1 (en) 2021-03-09 2022-09-15 Illumina, Inc. Analyzing expression of protein-coding variants in cells
CA3209074A1 (en) 2021-03-09 2022-09-15 Andrew Kennedy Genomic library preparation and targeted epigenetic assays using cas-grna ribonucleoproteins
WO2023018730A1 (en) 2021-08-11 2023-02-16 Illumina, Inc. Detection of analytes using targeted epigenetic assays, proximity-induced tagmentation, strand invasion, restriction, or ligation
WO2023034489A1 (en) 2021-09-01 2023-03-09 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
CN113981041B (zh) * 2021-11-25 2024-04-30 首都医科大学附属北京安贞医院 靶向富集测序试剂及靶向富集方法
WO2023189403A1 (ja) * 2022-03-31 2023-10-05 国立大学法人埼玉大学 Ｒｎａ／ｄｎａキメラプライマーを用いたｐｃｒ及びその増幅断片連結方法

Family Cites Families (58)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS57501692A (ja) 1980-09-24 1982-09-16
US4582788A (en) 1982-01-22 1986-04-15 Cetus Corporation HLA typing method and cDNA probes used therein
DE3381518D1 (de) 1982-01-22 1990-06-07 Cetus Corp Verfahren zur charakterisierung von hla und die darin benutzten cdns-testmittel.
US4786600A (en) 1984-05-25 1988-11-22 The Trustees Of Columbia University In The City Of New York Autocatalytic replication of recombinant RNA
US4683194A (en) 1984-05-29 1987-07-28 Cetus Corporation Method for detection of polymorphic restriction sites and nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
CA1284931C (en) 1986-03-13 1991-06-18 Henry A. Erlich Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids
CA1338457C (en) 1986-08-22 1996-07-16 Henry A. Erlich Purified thermostable enzyme
DE3634356A1 (de) 1986-10-08 1988-04-21 Epis Sa Arzneimittel enthaltend alpha-halogenierte dicarbonsaeuren
IL86724A (en) 1987-06-19 1995-01-24 Siska Diagnostics Inc Methods and kits for amplification and testing of nucleic acid sequences
CA1340843C (en) 1987-07-31 1999-12-07 J. Lawrence Burg Selective amplification of target polynucleotide sequences
US6004745A (en) 1987-09-21 1999-12-21 Gen-Probe Incorporated Hybridization protection assay
AU622426B2 (en) 1987-12-11 1992-04-09 Abbott Laboratories Assay using template-dependent nucleic acid probe reorganization
JP2650159B2 (ja) 1988-02-24 1997-09-03 アクゾ・ノベル・エヌ・ベー 核酸増幅方法
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
US5508178A (en) 1989-01-19 1996-04-16 Rose; Samuel Nucleic acid amplification using single primer
US5766849A (en) 1989-07-11 1998-06-16 Gen-Probe Incorporated Methods of amplifying nucleic acids using promoter-containing primer sequence
CA2020958C (en) 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
ATE141956T1 (de) 1989-07-11 1996-09-15 Gen Probe Inc Verfahren zur vervielfältigung von nukleinsäuresequenzen
US5545522A (en) * 1989-09-22 1996-08-13 Van Gelder; Russell N. Process for amplifying a target polynucleotide sequence using a single primer-promoter complex
US6013431A (en) 1990-02-16 2000-01-11 Molecular Tool, Inc. Method for determining specific nucleotide variations by primer extension in the presence of mixture of labeled nucleotides and terminators
US5427911A (en) 1990-05-01 1995-06-27 Yale University Coupled amplification and sequencing of DNA
HU218095B (hu) 1990-05-01 2000-05-28 Amgen Inc. Eljárás átvitt szennyeződések csökkentésére, amplifikációs eljárásokban
US5194370A (en) 1990-05-16 1993-03-16 Life Technologies, Inc. Promoter ligation activated transcription amplification of nucleic acid sequences
US5595891A (en) 1990-07-19 1997-01-21 Behringwerke Ag Method for producing a polynucleotide for use in single primer amplification
US5527872A (en) 1990-09-14 1996-06-18 At&T Global Information Solutions Company Electronic device with a spin-on glass dielectric layer
US5846710A (en) 1990-11-02 1998-12-08 St. Louis University Method for the detection of genetic diseases and gene sequence variations by single nucleotide primer extension
US6004744A (en) 1991-03-05 1999-12-21 Molecular Tool, Inc. Method for determining nucleotide identity through extension of immobilized primer
US5888819A (en) 1991-03-05 1999-03-30 Molecular Tool, Inc. Method for determining nucleotide identity through primer extension
US5169766A (en) 1991-06-14 1992-12-08 Life Technologies, Inc. Amplification of nucleic acid molecules
US5270184A (en) * 1991-11-19 1993-12-14 Becton, Dickinson And Company Nucleic acid target generation
WO1993022461A1 (en) 1992-05-06 1993-11-11 Gen-Probe Incorporated Nucleic acid sequence amplification method, composition and kit
US5710028A (en) 1992-07-02 1998-01-20 Eyal; Nurit Method of quick screening and identification of specific DNA sequences by single nucleotide primer extension and kits therefor
ATE152180T1 (de) 1992-07-31 1997-05-15 Behringwerke Ag Verfahren zur einführung von definierten sequenzen am 3' ende von polynukleotiden
ZA936016B (en) 1992-08-24 1994-03-10 Akzo Nv Method for nucleic acid amplification
JPH0723799A (ja) * 1993-07-13 1995-01-27 Hitachi Ltd ポリヌクレオチドの検出方法
US6027923A (en) 1993-07-23 2000-02-22 Bio-Rad Laboratories, Inc. Linked linear amplification of nucleic acids
FR2708288B1 (fr) * 1993-07-26 1995-09-01 Bio Merieux Procédé d'amplification d'acides nucléiques par transcription utilisant le déplacement, réactifs et nécessaire pour la mise en Óoeuvre de ce procédé.
US5648211A (en) 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
FR2724934B1 (fr) 1994-09-26 1997-01-24 Bio Merieux Oligonucleotide chimere et son utilisation dans l'obtention de transcrits d'un acide nucleique
US5665545A (en) * 1994-11-28 1997-09-09 Akzo Nobel N.V. Terminal repeat amplification method
US5882867A (en) 1995-06-07 1999-03-16 Dade Behring Marburg Gmbh Detection of nucleic acids by formation of template-dependent product
US5989813A (en) * 1995-07-13 1999-11-23 Molecular Innovations, Inc. Detection of amplified nucleic acid sequences using bifunctional haptenization and dyed microparticles
FR2737223B1 (fr) * 1995-07-24 1997-09-12 Bio Merieux Procede d'amplification de sequences d'acide nucleique par deplacement, a l'aide d'amorces chimeres
US5747255A (en) * 1995-09-29 1998-05-05 Lynx Therapeutics, Inc. Polynucleotide detection by isothermal amplification using cleavable oligonucleotides
US5871697A (en) 1995-10-24 1999-02-16 Curagen Corporation Method and apparatus for identifying, classifying, or quantifying DNA sequences in a sample without sequencing
ATE203056T1 (de) 1995-12-15 2001-07-15 Amersham Life Science Inc Thermostabile dna polymerase aus thermoanaerobacter thermohydrosulfuricus und davon abgeleitete mutierte enzyme mit entfernter exonukleaseaktivität
US5962271A (en) 1996-01-03 1999-10-05 Cloutech Laboratories, Inc. Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end
CA2255774C (en) 1996-05-29 2008-03-18 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
US6482590B1 (en) 1996-12-20 2002-11-19 Aventis Behring Gmbh Method for polynucleotide amplification
DE19653439A1 (de) 1996-12-20 1998-07-02 Svante Dr Paeaebo Verfahren zur direkten, exponentiellen Amplifikation und Sequenzierung von DNA Molekülen und dessen Anwendung
US6124120A (en) 1997-10-08 2000-09-26 Yale University Multiple displacement amplification
WO1999029901A1 (en) * 1997-12-11 1999-06-17 The General Hospital Corporation Broad range pcr amplification techniques
JP4317953B2 (ja) * 1998-01-22 2009-08-19 独立行政法人理化学研究所 Ｄｎａの塩基配列決定方法
EP1055001A1 (en) * 1998-02-05 2000-11-29 Bavarian Nordic Research Institute A/S Quantification by inhibition of amplification
US6365346B1 (en) 1998-02-18 2002-04-02 Dade Behring Inc. Quantitative determination of nucleic acid amplification products
ES2369818T3 (es) 1998-11-09 2011-12-07 Eiken Kagaku Kabushiki Kaisha Procedimiento de síntesis de ácido nucleico.
WO2000070095A2 (en) * 1999-05-17 2000-11-23 Dade Behring Inc. Homogeneous isothermal amplification and detection of nucleic acids using a template switch oligonucleotide

Also Published As

Publication number Publication date
ES2214319T3 (es) 2004-09-16
IL148091A0 (en) 2002-09-12
DK1431303T3 (en) 2014-02-17
JP3929775B2 (ja) 2007-06-13
EP1431303B1 (en) 2013-12-11
ES2447419T3 (es) 2014-03-12
JP2003509068A (ja) 2003-03-11
HK1046021B (zh) 2004-10-21
PT1218542E (pt) 2004-08-31
KR20020047160A (ko) 2002-06-21
AU7483500A (en) 2001-04-17
WO2001020035A3 (en) 2001-12-06
CA2384838A1 (en) 2001-03-22
CN1373812B (zh) 2012-04-11
MXPA02002656A (es) 2003-10-14
EP1431303A2 (en) 2004-06-23
HK1046021A1 (en) 2002-12-20
NZ517121A (en) 2004-05-28
US6251639B1 (en) 2001-06-26
BR0014182A (pt) 2002-05-21
US20010034048A1 (en) 2001-10-25
DE60009323T2 (de) 2005-02-10
WO2001020035A2 (en) 2001-03-22
EP1218542A2 (en) 2002-07-03
NO20021223D0 (no) 2002-03-12
CA2384838C (en) 2006-07-18
NO20021223L (no) 2002-05-13
CN102586228A (zh) 2012-07-18
ATE262595T1 (de) 2004-04-15
EP1431303A3 (en) 2010-01-06
DE60009323D1 (de) 2004-04-29
EP1218542B1 (en) 2004-03-24
AU783873B2 (en) 2005-12-15
WO2001020035A9 (en) 2002-11-07
KR100527265B1 (ko) 2005-11-09
CN1373812A (zh) 2002-10-09
JP2003116586A (ja) 2003-04-22

Similar Documents

Publication Publication Date Title
DK1218542T3 (da) Fremgangsmåder og sammensætninger til lineær isotermisk amplifikation af polynukleotidsekvenser
DK0973940T3 (da) En in vitro-fremgangsmåde til konstruktion af et DNA-bibliotek
WO2002052031A3 (en) Nucleic acid amplification
CA2532160A1 (en) Methods of genotyping using differences in melting temperature
DE60038796D1 (de) Hochspezifische primer, amplifizierungsverfahren und kits
EP1350841A3 (en) Thermostable or thermoactive DNA polymerase with attenuated 3'-5' exonuclease activity
WO2004074503A3 (en) Nucleic acid sequencing methods, kits and reagents
DE60123980D1 (de) Amplifizierung von nukleinsäuresequenzen mittels multiplex priming
WO2004092418A3 (en) Global amplification using a randomly primed composite primer
ATE400663T1 (de) Rekombinase-polymerase-amplifikation
WO2005044836A3 (de) Makromolekulare nukleotidverbindungen und methoden zu deren anwendung
AU6846698A (en) Method of nucleic acid amplification
DK1815013T3 (da) Sammensætning til at amplificere nucleinsyrer
EP1055736A4 (en) METHOD FOR DNA SEQUENCING
ATE361377T1 (de) Leistungstarke verknüpfung von nukleinsäuresegmenten
WO2005054438A3 (en) Nucleic acid molecules containing recombination sites and methods of using the same
WO2004104229A3 (en) Internal control nucleic acid molecule for nucleic acid amplification systems
WO2003000927A3 (en) Method for the determination of telomere length
DK1947104T3 (da) Fremgangsmåder og sammensætninger til identifikation og karakterisering af hepatitis C
ATE539167T1 (de) Verfahren zur amplifikation von rna-sequenzen
WO2006081463A3 (en) Thermococcus zilligii dna polymerases and variants thereof
AR027256A1 (es) Deteccion de microorganismos relacionados con la fermentacion
DE602004019059D1 (de) Methode zur linearen nicht-selektiven amplifikation von nukleinsäuren
DE60024679D1 (de) Methoden und zusammensetzungen zur detektion von spezies des mycobacterium avium-komplexes
DE50312117D1 (de) Verfahren zur Amplifikation genetischer Information mittels an mehrere Stellen im Genom bindende Primer