ATE152180T1 - Verfahren zur einführung von definierten sequenzen am 3' ende von polynukleotiden - Google Patents
Verfahren zur einführung von definierten sequenzen am 3' ende von polynukleotidenInfo
- Publication number
- ATE152180T1 ATE152180T1 AT93917347T AT93917347T ATE152180T1 AT E152180 T1 ATE152180 T1 AT E152180T1 AT 93917347 T AT93917347 T AT 93917347T AT 93917347 T AT93917347 T AT 93917347T AT E152180 T1 ATE152180 T1 AT E152180T1
- Authority
- AT
- Austria
- Prior art keywords
- polynucleotide
- primer
- sequences
- sequence
- blocker
- Prior art date
Links
- 102000040430 polynucleotide Human genes 0.000 title abstract 15
- 108091033319 polynucleotide Proteins 0.000 title abstract 15
- 239000002157 polynucleotide Substances 0.000 title abstract 15
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 230000000295 complement effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US92307992A | 1992-07-31 | 1992-07-31 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE152180T1 true ATE152180T1 (de) | 1997-05-15 |
Family
ID=25448085
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT93917347T ATE152180T1 (de) | 1992-07-31 | 1993-07-30 | Verfahren zur einführung von definierten sequenzen am 3' ende von polynukleotiden |
Country Status (9)
| Country | Link |
|---|---|
| US (3) | US5679512A (de) |
| EP (1) | EP0652973B1 (de) |
| JP (1) | JPH07509365A (de) |
| AT (1) | ATE152180T1 (de) |
| CA (1) | CA2141450A1 (de) |
| DE (1) | DE69310179T2 (de) |
| DK (1) | DK0652973T3 (de) |
| ES (1) | ES2104160T3 (de) |
| WO (1) | WO1994003637A1 (de) |
Families Citing this family (94)
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| EP2218732A1 (de) * | 1997-11-28 | 2010-08-18 | Merck Serono Biodevelopment | Genomsequenz von Chlamydia trachomatis sowie Polypeptide, Fragmente davon und deren Verwendung, insbesondere zur Diagnose, Prävention und Behandlung einer Infektion |
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| EP1171634B1 (de) * | 1999-04-08 | 2005-10-26 | Gen-Probe Incorporated | Amplifikation und sequenzierung von primer-paaren und deren verwendung |
| WO2000061810A1 (en) * | 1999-04-08 | 2000-10-19 | Oasis Biosciences, Inc. | Antisense oligonucleotides comprising universal and/or degenerate bases |
| US6692918B2 (en) | 1999-09-13 | 2004-02-17 | Nugen Technologies, Inc. | Methods and compositions for linear isothermal amplification of polynucleotide sequences |
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| US5043272A (en) * | 1989-04-27 | 1991-08-27 | Life Technologies, Incorporated | Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers |
| US5595891A (en) * | 1990-07-19 | 1997-01-21 | Behringwerke Ag | Method for producing a polynucleotide for use in single primer amplification |
| EP0666927B1 (de) * | 1991-09-13 | 1997-05-07 | Cytocell Limited | Verfahren zur Bestimmung von einer Nukleinsäuresequenz |
| US5424413A (en) * | 1992-01-22 | 1995-06-13 | Gen-Probe Incorporated | Branched nucleic acid probes |
-
1993
- 1993-07-30 AT AT93917347T patent/ATE152180T1/de active
- 1993-07-30 DK DK93917347.2T patent/DK0652973T3/da active
- 1993-07-30 ES ES93917347T patent/ES2104160T3/es not_active Expired - Lifetime
- 1993-07-30 EP EP93917347A patent/EP0652973B1/de not_active Expired - Lifetime
- 1993-07-30 WO PCT/US1993/007051 patent/WO1994003637A1/en not_active Ceased
- 1993-07-30 CA CA002141450A patent/CA2141450A1/en not_active Abandoned
- 1993-07-30 JP JP6505390A patent/JPH07509365A/ja not_active Ceased
- 1993-07-30 DE DE69310179T patent/DE69310179T2/de not_active Expired - Fee Related
- 1993-10-20 US US08/140,349 patent/US5679512A/en not_active Expired - Fee Related
-
1995
- 1995-06-07 US US08/479,745 patent/US6030774A/en not_active Expired - Fee Related
- 1995-06-07 US US08/475,236 patent/US5683879A/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CA2141450A1 (en) | 1994-02-17 |
| ES2104160T3 (es) | 1997-10-01 |
| WO1994003637A1 (en) | 1994-02-17 |
| EP0652973B1 (de) | 1997-04-23 |
| DK0652973T3 (da) | 1997-09-15 |
| US6030774A (en) | 2000-02-29 |
| EP0652973A1 (de) | 1995-05-17 |
| DE69310179T2 (de) | 1997-07-31 |
| DE69310179D1 (de) | 1997-05-28 |
| JPH07509365A (ja) | 1995-10-19 |
| US5683879A (en) | 1997-11-04 |
| US5679512A (en) | 1997-10-21 |
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