DK0652973T3 - Fremgangsmåde til indføring af definerede sekvenser ved 3-enden af polynukleotider - Google Patents
Fremgangsmåde til indføring af definerede sekvenser ved 3-enden af polynukleotiderInfo
- Publication number
- DK0652973T3 DK0652973T3 DK93917347.2T DK93917347T DK0652973T3 DK 0652973 T3 DK0652973 T3 DK 0652973T3 DK 93917347 T DK93917347 T DK 93917347T DK 0652973 T3 DK0652973 T3 DK 0652973T3
- Authority
- DK
- Denmark
- Prior art keywords
- polynucleotide
- primer
- sequences
- sequence
- blocker
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US92307992A | 1992-07-31 | 1992-07-31 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK0652973T3 true DK0652973T3 (da) | 1997-09-15 |
Family
ID=25448085
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK93917347.2T DK0652973T3 (da) | 1992-07-31 | 1993-07-30 | Fremgangsmåde til indføring af definerede sekvenser ved 3-enden af polynukleotider |
Country Status (9)
| Country | Link |
|---|---|
| US (3) | US5679512A (de) |
| EP (1) | EP0652973B1 (de) |
| JP (1) | JPH07509365A (de) |
| AT (1) | ATE152180T1 (de) |
| CA (1) | CA2141450A1 (de) |
| DE (1) | DE69310179T2 (de) |
| DK (1) | DK0652973T3 (de) |
| ES (1) | ES2104160T3 (de) |
| WO (1) | WO1994003637A1 (de) |
Families Citing this family (87)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU7238396A (en) * | 1995-09-12 | 1997-04-01 | Becton Dickinson & Company | Device and method for dna amplification and assay |
| US6482590B1 (en) | 1996-12-20 | 2002-11-19 | Aventis Behring Gmbh | Method for polynucleotide amplification |
| US6518017B1 (en) * | 1997-10-02 | 2003-02-11 | Oasis Biosciences Incorporated | Combinatorial antisense library |
| US20030165888A1 (en) * | 2001-07-18 | 2003-09-04 | Brown Bob D. | Oligonucleotide probes and primers comprising universal bases for diagnostic purposes |
| AU754264B2 (en) * | 1997-11-28 | 2002-11-07 | Genset S.A. | Chlamydia trachomatis genomic sequence and polypeptides, fragments thereof and uses thereof, in particular for the diagnosis, prevention and treatment of infection |
| US6289229B1 (en) * | 1998-01-20 | 2001-09-11 | Scimed Life Systems, Inc. | Readable probe array for in vivo use |
| WO1999037806A2 (en) * | 1998-01-27 | 1999-07-29 | Cytocell Limited | Modified nucleic acid probes and uses thereof |
| CA2365980A1 (en) * | 1999-04-08 | 2000-10-19 | Oasis Biosciences, Inc. | Amplification and sequencing primer pairs and use thereof |
| WO2000061810A1 (en) * | 1999-04-08 | 2000-10-19 | Oasis Biosciences, Inc. | Antisense oligonucleotides comprising universal and/or degenerate bases |
| US6692918B2 (en) * | 1999-09-13 | 2004-02-17 | Nugen Technologies, Inc. | Methods and compositions for linear isothermal amplification of polynucleotide sequences |
| ES2447419T3 (es) | 1999-09-13 | 2014-03-12 | Nugen Technologies, Inc. | Composiciones para la amplificación isotérmica lineal de secuencias de polinucleótidos |
| DE60141087D1 (de) * | 2000-06-26 | 2010-03-04 | Nugen Technologies Inc | Methoden und zusammensetzungen zur auf transkription basierenden vervielfältigung von nukleinsäuren |
| US7846733B2 (en) | 2000-06-26 | 2010-12-07 | Nugen Technologies, Inc. | Methods and compositions for transcription-based nucleic acid amplification |
| US6994963B1 (en) * | 2000-07-10 | 2006-02-07 | Ambion, Inc. | Methods for recombinatorial nucleic acid synthesis |
| WO2002029117A2 (en) | 2000-10-06 | 2002-04-11 | Nugen Technologies, Inc. | Methods and probes for detection and/or quantification of nucleic acid sequences |
| CA2436693A1 (en) | 2000-12-11 | 2002-06-20 | Alexion Pharmaceuticals, Inc. | Nested oligonucleotides containing hairpin for nucleic acid amplification |
| EP1427847B1 (de) | 2000-12-13 | 2010-07-28 | Nugen Technologies, Inc. | Methoden und zusammensetzungen zur generierung einer vielzahl von kopien von nukleinsäuresequenzen und methoden zur detektion derselben |
| CN1289690C (zh) | 2001-03-09 | 2006-12-13 | 纽亘技术公司 | Rna序列扩增的方法和组合物 |
| CA2439074A1 (en) | 2001-03-09 | 2002-09-19 | Nugen Technologies, Inc. | Methods and compositions for amplification of rna sequences |
| US20040175715A1 (en) * | 2001-08-21 | 2004-09-09 | Burgoyne Leigh A. | Method and device for simultaneously molecularly cloning and polylocus profiling of genomes or genomes mixtures |
| US7414111B2 (en) * | 2001-09-19 | 2008-08-19 | Alexion Pharmaceuticals, Inc. | Engineered templates and their use in single primer amplification |
| CA2460639C (en) * | 2001-09-19 | 2013-07-16 | Alexion Pharmaceuticals, Inc. | Engineered templates and their use in single primer amplification |
| US7045319B2 (en) * | 2001-10-30 | 2006-05-16 | Ribomed Biotechnologies, Inc. | Molecular detection systems utilizing reiterative oligonucleotide synthesis |
| US20040054162A1 (en) * | 2001-10-30 | 2004-03-18 | Hanna Michelle M. | Molecular detection systems utilizing reiterative oligonucleotide synthesis |
| ATE405676T1 (de) * | 2002-03-11 | 2008-09-15 | Nugen Technologies Inc | Verfahren zur erzeugung doppelsträngiger dna mit einem 3'-einzelstranganteil und verwendungen dieser komplexe zur rekombination |
| JP4551216B2 (ja) * | 2002-05-17 | 2010-09-22 | ニューゲン テクノロジーズ， インコーポレイテッド | 核酸の断片化、標識および固定化の方法 |
| WO2004048594A2 (en) | 2002-11-21 | 2004-06-10 | Epicentre Technologies | Preparation and use of single-stranded transcription substrates for synthesis of transcription products corresponding to target sequences |
| CA2521084A1 (en) | 2003-04-14 | 2004-10-28 | Nugen Technologies, Inc. | Global amplification using a randomly primed composite primer |
| JP2007521018A (ja) * | 2003-10-29 | 2007-08-02 | リボメド バイオテクノロジーズ，インコーポレイテッド | 反復的オリゴヌクレオチド合成のための組成物、方法、および検出技術 |
| WO2005065321A2 (en) | 2003-12-29 | 2005-07-21 | Nugen Technologies, Inc. | Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids |
| BRPI0607378A2 (pt) | 2005-02-26 | 2010-03-23 | Basf Plant Science Gmbh | cassetes de expressço, seqÜÊncia nucleotÍdica isolada, vetor, cÉlula hospedeira transgÊnica ou organismo nço humano, cÉlula de planta ou planta transgÊnica, mÉtodos para identificar e/ou isolar uma seqÜÊncia nucleotÍdica reguladora da transcriÇço, para prover ou produzir um cassete de expressço transgÊnica, e para prover uma seqÜÊncia nucleotÍdica reguladora de transcriÇço sintÉtica, e, seqÜÊncia reguladora de transcriÇço sintÉtica |
| WO2007030759A2 (en) | 2005-09-07 | 2007-03-15 | Nugen Technologies, Inc. | Improved nucleic acid amplification procedure |
| DE102006020885A1 (de) * | 2006-05-05 | 2007-11-08 | Qiagen Gmbh | Einführung von Sequenzelementen in Nukleinsäuren |
| CA2656315A1 (en) * | 2006-06-30 | 2008-01-10 | Nugen Technologies, Inc. | Methods for fragmentation and labeling of nucleic acids |
| CA2707436C (en) | 2007-06-29 | 2014-01-28 | Epicentre Technologies Corporation | Copy dna and sense rna |
| US20090203531A1 (en) * | 2008-02-12 | 2009-08-13 | Nurith Kurn | Method for Archiving and Clonal Expansion |
| GB2470672B (en) | 2008-03-21 | 2012-09-12 | Nugen Technologies Inc | Methods of RNA amplification in the presence of DNA |
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| DK2501716T3 (en) | 2009-11-19 | 2015-04-07 | Solis Biodyne Oü | Formations to increase polypeptidstabilitet and activity and related practices |
| US9074246B2 (en) | 2010-01-25 | 2015-07-07 | Rd Biosciences, Inc. | Self-folding amplification of target nucleic acid |
| CN102741429A (zh) * | 2010-02-25 | 2012-10-17 | 松下电器产业株式会社 | 对双链dna中的目标序列进行扩增的方法 |
| EP3578205A1 (de) | 2010-08-06 | 2019-12-11 | ModernaTX, Inc. | Pharmazeutische zusammensetzungen enthaltenbearbeitete nukleinsäuren und ihre medizinische verwendung |
| CA2813466A1 (en) | 2010-10-01 | 2012-04-05 | Moderna Therapeutics, Inc. | Modified nucleosides, nucleotides, and nucleic acids that disrupt major groove binding partner interactions |
| EP2691101A2 (de) | 2011-03-31 | 2014-02-05 | Moderna Therapeutics, Inc. | Freisetzung und formulierung von manipulierten nukleinsäuren |
| US9464124B2 (en) | 2011-09-12 | 2016-10-11 | Moderna Therapeutics, Inc. | Engineered nucleic acids and methods of use thereof |
| KR102014061B1 (ko) | 2011-10-03 | 2019-08-28 | 모더나 세라퓨틱스, 인코포레이티드 | 변형된 뉴클레오사이드, 뉴클레오타이드, 및 핵산, 및 이들의 용도 |
| WO2013059746A1 (en) | 2011-10-19 | 2013-04-25 | Nugen Technologies, Inc. | Compositions and methods for directional nucleic acid amplification and sequencing |
| LT2791160T (lt) | 2011-12-16 | 2022-06-10 | Modernatx, Inc. | Modifikuotos mrnr sudėtys |
| EP3578697B1 (de) | 2012-01-26 | 2024-03-06 | Tecan Genomics, Inc. | Zusammensetzungen und verfahren zur gezielten nukleinsäuresequenzanreicherung und hocheffizienten erzeugung einer bibliothek |
| AU2013243947A1 (en) | 2012-04-02 | 2014-10-30 | Moderna Therapeutics, Inc. | Modified polynucleotides for the production of proteins |
| US9254311B2 (en) | 2012-04-02 | 2016-02-09 | Moderna Therapeutics, Inc. | Modified polynucleotides for the production of proteins |
| US9283287B2 (en) | 2012-04-02 | 2016-03-15 | Moderna Therapeutics, Inc. | Modified polynucleotides for the production of nuclear proteins |
| US9572897B2 (en) | 2012-04-02 | 2017-02-21 | Modernatx, Inc. | Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins |
| CN104619894B (zh) | 2012-06-18 | 2017-06-06 | 纽亘技术公司 | 用于非期望核酸序列的阴性选择的组合物和方法 |
| US20150011396A1 (en) | 2012-07-09 | 2015-01-08 | Benjamin G. Schroeder | Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing |
| CA2892529C (en) | 2012-11-26 | 2023-04-25 | Moderna Therapeutics, Inc. | Terminally modified rna |
| AU2013352067A1 (en) | 2012-11-28 | 2015-06-04 | Abwiz Bio, Inc. | Preparation of gene-specific templates for the use in single primer amplification |
| US9822408B2 (en) | 2013-03-15 | 2017-11-21 | Nugen Technologies, Inc. | Sequential sequencing |
| US8980864B2 (en) | 2013-03-15 | 2015-03-17 | Moderna Therapeutics, Inc. | Compositions and methods of altering cholesterol levels |
| EP3722442B1 (de) | 2013-08-05 | 2023-04-05 | Twist Bioscience Corporation | De-novo-synthetisierte genbanken |
| MX2016004249A (es) | 2013-10-03 | 2016-11-08 | Moderna Therapeutics Inc | Polinulcleotidos que codifican el receptor de lipoproteina de baja densidad. |
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| IL258164B (en) | 2015-09-18 | 2022-09-01 | Twist Bioscience Corp | Methods to regulate the activity of proteins and cells and a method for the production of nucleic acids |
| KR20180058772A (ko) | 2015-09-22 | 2018-06-01 | 트위스트 바이오사이언스 코포레이션 | 핵산 합성을 위한 가요성 기판 |
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| KR102217487B1 (ko) | 2016-09-21 | 2021-02-23 | 트위스트 바이오사이언스 코포레이션 | 핵산 기반 데이터 저장 |
| CN110366613A (zh) | 2016-12-16 | 2019-10-22 | 特韦斯特生物科学公司 | 免疫突触的变体文库及其合成 |
| CA3054303A1 (en) | 2017-02-22 | 2018-08-30 | Twist Bioscience Corporation | Nucleic acid based data storage |
| AU2018234629A1 (en) | 2017-03-15 | 2019-10-17 | Twist Bioscience Corporation | Variant libraries of the immunological synapse and synthesis thereof |
| KR20240013290A (ko) | 2017-06-12 | 2024-01-30 | 트위스트 바이오사이언스 코포레이션 | 심리스 핵산 어셈블리를 위한 방법 |
| WO2018231864A1 (en) | 2017-06-12 | 2018-12-20 | Twist Bioscience Corporation | Methods for seamless nucleic acid assembly |
| CA3075505A1 (en) | 2017-09-11 | 2019-03-14 | Twist Bioscience Corporation | Gpcr binding proteins and synthesis thereof |
| US11099202B2 (en) | 2017-10-20 | 2021-08-24 | Tecan Genomics, Inc. | Reagent delivery system |
| JP7066840B2 (ja) | 2017-10-20 | 2022-05-13 | ツイスト バイオサイエンス コーポレーション | ポリヌクレオチド合成のための加熱されたナノウェル |
| JP7175326B2 (ja) | 2017-11-29 | 2022-11-18 | パナジーン・インコーポレイテッド | 標的核酸増幅方法及び標的核酸増幅用組成物 |
| SG11202006460SA (en) | 2018-01-04 | 2020-08-28 | Twist Bioscience Corp | Dna-based digital information storage |
| WO2019222706A1 (en) | 2018-05-18 | 2019-11-21 | Twist Bioscience Corporation | Polynucleotides, reagents, and methods for nucleic acid hybridization |
| US20210002710A1 (en) * | 2019-02-25 | 2021-01-07 | Twist Bioscience Corporation | Compositions and methods for next generation sequencing |
| AU2020229349A1 (en) | 2019-02-26 | 2021-10-14 | Twist Bioscience Corporation | Variant nucleic acid libraries for GLP1 receptor |
| WO2020176680A1 (en) | 2019-02-26 | 2020-09-03 | Twist Bioscience Corporation | Variant nucleic acid libraries for antibody optimization |
| AU2020298294A1 (en) | 2019-06-21 | 2022-02-17 | Twist Bioscience Corporation | Barcode-based nucleic acid sequence assembly |
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1993
- 1993-07-30 AT AT93917347T patent/ATE152180T1/de active
- 1993-07-30 CA CA002141450A patent/CA2141450A1/en not_active Abandoned
- 1993-07-30 DK DK93917347.2T patent/DK0652973T3/da active
- 1993-07-30 EP EP93917347A patent/EP0652973B1/de not_active Expired - Lifetime
- 1993-07-30 WO PCT/US1993/007051 patent/WO1994003637A1/en active IP Right Grant
- 1993-07-30 DE DE69310179T patent/DE69310179T2/de not_active Expired - Fee Related
- 1993-07-30 ES ES93917347T patent/ES2104160T3/es not_active Expired - Lifetime
- 1993-07-30 JP JP6505390A patent/JPH07509365A/ja not_active Ceased
- 1993-10-20 US US08/140,349 patent/US5679512A/en not_active Expired - Fee Related
-
1995
- 1995-06-07 US US08/475,236 patent/US5683879A/en not_active Expired - Fee Related
- 1995-06-07 US US08/479,745 patent/US6030774A/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| DE69310179T2 (de) | 1997-07-31 |
| WO1994003637A1 (en) | 1994-02-17 |
| JPH07509365A (ja) | 1995-10-19 |
| EP0652973B1 (de) | 1997-04-23 |
| DE69310179D1 (de) | 1997-05-28 |
| ES2104160T3 (es) | 1997-10-01 |
| EP0652973A1 (de) | 1995-05-17 |
| US6030774A (en) | 2000-02-29 |
| US5679512A (en) | 1997-10-21 |
| ATE152180T1 (de) | 1997-05-15 |
| CA2141450A1 (en) | 1994-02-17 |
| US5683879A (en) | 1997-11-04 |
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