DD257087A5 - Verfahren zur Herstellung von biologisch aktivem Plasminogenaktivator - Google Patents

Verfahren zur Herstellung von biologisch aktivem Plasminogenaktivator Download PDF

Info

Publication number
DD257087A5
DD257087A5 DD30357587A DD30357587A DD257087A5 DD 257087 A5 DD257087 A5 DD 257087A5 DD 30357587 A DD30357587 A DD 30357587A DD 30357587 A DD30357587 A DD 30357587A DD 257087 A5 DD257087 A5 DD 257087A5
Authority
DD
German Democratic Republic
Prior art keywords
plasminogen activator
human tissue
medium
host cell
cell culture
Prior art date
Application number
DD30357587A
Other languages
German (de)
English (en)
Inventor
William R Arathoon
Stuart E Builder
Anthony S Lubiniecki
Robert D Van Reis
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Publication of DD257087A5 publication Critical patent/DD257087A5/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Mycology (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Diabetes (AREA)
  • Public Health (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DD30357587A 1986-06-06 1987-06-05 Verfahren zur Herstellung von biologisch aktivem Plasminogenaktivator DD257087A5 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US87164286A 1986-06-06 1986-06-06

Publications (1)

Publication Number Publication Date
DD257087A5 true DD257087A5 (de) 1988-06-01

Family

ID=25357832

Family Applications (1)

Application Number Title Priority Date Filing Date
DD30357587A DD257087A5 (de) 1986-06-06 1987-06-05 Verfahren zur Herstellung von biologisch aktivem Plasminogenaktivator

Country Status (21)

Country Link
US (1) US5053334A (cs)
EP (1) EP0248675B1 (cs)
JP (1) JPS6332484A (cs)
KR (1) KR930002888B1 (cs)
AT (1) ATE75775T1 (cs)
AU (1) AU601984B2 (cs)
DD (1) DD257087A5 (cs)
DE (2) DE3718939C2 (cs)
DK (1) DK175366B1 (cs)
ES (1) ES2032444T3 (cs)
FI (1) FI91886C (cs)
FR (1) FR2599625B1 (cs)
GB (1) GB2191493B (cs)
GR (1) GR3005245T3 (cs)
HU (1) HU205171B (cs)
IE (1) IE60485B1 (cs)
IL (1) IL82746A (cs)
NO (1) NO170595C (cs)
NZ (1) NZ220547A (cs)
PT (1) PT84991B (cs)
ZA (1) ZA874054B (cs)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL82746A (en) * 1986-06-06 1994-10-07 Genentech Inc Process for producing biologically active tissue-type plasminogen activator
JP2576200B2 (ja) * 1988-03-09 1997-01-29 味の素株式会社 生理活性タンパク質の製造法
EP0338716A3 (en) * 1988-04-21 1990-04-11 Berlex Laboratories, Inc. Method and system for the production of non-degraded proteins from mammalian cells
DE3829244A1 (de) * 1988-08-29 1990-03-01 Boehringer Mannheim Gmbh Verfahren zur herstellung von einkettigem t-pa
EP0391080A3 (de) * 1989-03-07 1990-11-07 Roche Diagnostics GmbH Verfahren zur Herstellung von zweikettigem t-PA
GB2249099B (en) * 1990-09-26 1995-05-03 Squibb & Sons Inc Squalene synthetase
US5677184A (en) * 1994-04-19 1997-10-14 Takeda Chemical Industries, Ltd. CHO cells that express human LH-RH receptor
US6077940A (en) 1997-12-24 2000-06-20 Genentech, Inc. Free solution ligand interaction molecular separation method
KR20050006147A (ko) 2002-04-08 2005-01-15 밀레늄 바이올로직스 인코포레이티드 자동화된 조직 엔지니어링 시스템
CN104263700A (zh) 2006-07-14 2015-01-07 帝斯曼知识产权资产管理有限公司 改进的细胞培养方法
EP2188371B1 (en) * 2007-08-09 2017-12-20 Wyeth LLC Use of perfusion to enhance production of fed-batch cell culture in bioreactors
EP2525899A1 (en) * 2010-01-22 2012-11-28 Lonza Walkersville, Inc. High yield method and apparatus for volume reduction and washing of therapeutic cells using tangential flow filtration
US10934519B2 (en) 2011-07-29 2021-03-02 Global Life Sciences Solutions Usa Llc Systems, methods and control laws for cell harvesting
US9695393B2 (en) * 2012-08-20 2017-07-04 Terumo Bct, Inc. Concentrating components of fluid circulated through a cell growth chamber
EP3662056A2 (en) 2017-09-01 2020-06-10 Lonza Walkersville, Inc. End-to-end cell therapy automation
CA3123449A1 (en) 2018-12-21 2020-06-25 Lonza Walkersville, Inc. Automated production of viral vectors
IL284214B2 (en) 2018-12-21 2025-03-01 Octane Biotech Inc Carousel for modular biologic production units
CA3123458A1 (en) 2018-12-28 2020-07-02 Octane Biotech Inc. Cell culture and tissue engineering systems with controlled environmental zones
KR20210125510A (ko) 2019-02-08 2021-10-18 론자 워커스빌 아이엔씨. 자동화 생물반응기에서 사용하기 위한 세포 농축 방법 및 장치
EP4048773A4 (en) 2019-10-24 2024-01-03 Octane Biotech Inc. CELL CULTURE CHAMBER WITH IMPROVED CELL CONTACT SURFACES
CA3159567A1 (en) 2019-11-11 2021-05-20 Lonza Walkersville, Inc. Quality control methods for automated cell processing
CA3224150A1 (en) 2021-07-02 2023-01-05 Combangio, Inc. Processes for making and using a cellular fibronectin composition

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3904480A (en) * 1972-10-24 1975-09-09 Lilly Co Eli Enhanced production of plasminogen activator
JPS5329985A (en) * 1976-08-27 1978-03-20 Kanegafuchi Chem Ind Co Ltd High concentration culturing of yeast
JPS5571496A (en) * 1978-11-24 1980-05-29 Asahi Chem Ind Co Ltd Preparation of living substance
US4409331A (en) * 1979-03-28 1983-10-11 Damon Corporation Preparation of substances with encapsulated cells
US4232124A (en) * 1979-04-23 1980-11-04 Mann George F Method of producing plasminogen activator
DE3015699C2 (de) * 1979-04-26 1982-07-15 Asahi Kasei Kogyo K.K., Osaka Herstellung eines Plasminogen-Aktivators
JPS5642584A (en) * 1979-09-18 1981-04-20 Asahi Chem Ind Co Ltd Cell cultivation method
US4420398A (en) * 1981-08-13 1983-12-13 American National Red Cross Filteration method for cell produced antiviral substances
GR79202B (cs) * 1982-05-05 1984-10-22 Genentech Inc
JPS5951220A (ja) * 1982-08-02 1984-03-24 Asahi Chem Ind Co Ltd 新規なプラスミノ−ゲン・アクチベ−タ−およびその製法ならびにこれを含有する薬剤
US4537860A (en) * 1982-12-08 1985-08-27 Monsanto Company Static cell culture maintenance system
NZ206699A (en) * 1982-12-30 1989-08-29 Bio Response Inc Process for the production of serum independent cell lines
AU572108B2 (en) * 1983-01-19 1988-05-05 Genentech Inc. Human tpa production using vectors coding for dhfr protein
JPS59121172U (ja) * 1983-02-04 1984-08-15 ミネソタ・マイニング・アンド・マニユフアクチユアリング・コンパニ− ケーブル電線被覆の剥離部分のカバー装置
US4546083A (en) * 1983-04-22 1985-10-08 Stolle Research & Development Corporation Method and device for cell culture growth
PH22337A (en) * 1983-11-21 1988-08-12 Ciba Geigy Ag Synthesis of fibrinolytic agents by yeast
US4740461A (en) * 1983-12-27 1988-04-26 Genetics Institute, Inc. Vectors and methods for transformation of eucaryotic cells
US4888115A (en) * 1983-12-29 1989-12-19 Cuno, Incorporated Cross-flow filtration
US4757005A (en) * 1984-04-19 1988-07-12 Miles Inc. Method and cell line for obtaining plasminogen activators
EP0215923B1 (en) * 1985-03-22 1990-12-27 Chiron Corporation Expression of tpa in mammalian cells
AU593264B2 (en) * 1985-07-10 1990-02-08 Kanegafuchi Kagaku Kogyo Kabushiki Kaisha Chromosomal DNA sequence, expression vector for human tissue plasminogen activating factor, cultured cells transfected with same and method of producing said activating factor
AU606582B2 (en) * 1985-09-06 1991-02-14 Codon Methods for the recovery of tissue plasminogen activator
JPH07110232B2 (ja) * 1985-10-14 1995-11-29 株式会社日立製作所 遺伝子組換え大腸菌の遺伝子生産物を採取する方法および装置
IL82746A (en) * 1986-06-06 1994-10-07 Genentech Inc Process for producing biologically active tissue-type plasminogen activator

Also Published As

Publication number Publication date
PT84991B (pt) 1990-03-08
NO872395L (no) 1987-12-07
DK175366B1 (da) 2004-09-13
PT84991A (en) 1987-07-01
FR2599625A1 (fr) 1987-12-11
JPS6332484A (ja) 1988-02-12
ZA874054B (en) 1989-02-22
NO170595B (no) 1992-07-27
GB2191493A (en) 1987-12-16
ATE75775T1 (de) 1992-05-15
KR880000590A (ko) 1988-03-28
HU205171B (en) 1992-03-30
AU601984B2 (en) 1990-09-27
KR930002888B1 (ko) 1993-04-12
DE3718939C2 (de) 1998-09-17
FR2599625B1 (fr) 1992-03-13
IL82746A0 (en) 1987-12-20
FI872526L (fi) 1987-12-07
GB8713267D0 (en) 1987-07-08
AU7387287A (en) 1987-12-10
IE871504L (en) 1987-12-06
GR3005245T3 (cs) 1993-05-24
JPH0574346B2 (cs) 1993-10-18
DK288187A (da) 1987-12-07
HUT43875A (en) 1987-12-28
IE60485B1 (en) 1994-07-27
NO872395D0 (no) 1987-06-05
NZ220547A (en) 1990-01-29
FI91886B (fi) 1994-05-13
NO170595C (no) 1992-11-04
IL82746A (en) 1994-10-07
ES2032444T3 (es) 1993-02-16
US5053334A (en) 1991-10-01
EP0248675B1 (en) 1992-05-06
EP0248675A1 (en) 1987-12-09
DE3718939A1 (de) 1987-12-17
FI91886C (fi) 1994-08-25
FI872526A0 (fi) 1987-06-05
GB2191493B (en) 1991-02-27
DK288187D0 (da) 1987-06-04
DE3778758D1 (de) 1992-06-11

Similar Documents

Publication Publication Date Title
DE3718939C2 (de) Verfahren zur Herstellung von biologisch aktivem Plasminogenaktivator
DE69715641T2 (de) Reinigung von biologisch aktiven peptiden aus milch
DE3027105C2 (cs)
DE69133442T2 (de) Wachstumsfördernder wirkstoff
US4533634A (en) Tissue culture medium
DE69634303T2 (de) Chondrogene in vitro induktion von menschlichen mensenchymalen stammzellen
DE69032600T2 (de) Rekombinanter abkömmling des menschlichen faktors iii
DE69434332T2 (de) Thrombin mutanten
DE69432859T2 (de) Verfahren zur reinigung von kollagenase
DE68911061T2 (de) Mittel zur Kontrolle von Thrombenbildungen.
DE69510669T2 (de) Modifizierter wachstumsfaktor der milch
EP0971958B1 (de) Verfahren zur reinigung von faktor viii/vwf-komplex mittels kationenaustauscherchromatographie
CA1200489A (en) Method of preparation of human urine origin colony- stimulating factor and kallikrein
EP0246439B1 (de) Steriles Plasmaaustauschmittel
EP0140134A2 (de) Verfahren zur Herstellung eines biologisch aktiven Extraktes
DE69321007T2 (de) Zusammensetzung zur Stabilisierung von Blutplasma im Laufe der Pasteurisation und Pasteurisationsverfahren unter Verwendung dieser Zusammensetzung
DE69311873T2 (de) Fed-batch-Verfahren für Proteine sekretierende Zellen
DE69719382T2 (de) VERFAHREN ZUR HERSTELLUNG VON rDSPA Alpha1
DE69117920T2 (de) Zusammensetzung zur stabilisierung von blutplasma im laufe der pasteurisation
EP0892045A2 (de) Verfahren zur Herstellung eines eine Hyaluronidase enthaltenden Präparats
EP0396597A1 (de) Verfahren zur herstellung eines therapeutisch aktiven, insbesondere zur wundheilung oder zur behandlung in der geriatrie verwendbaren wirkstoffes und ein einen solchen wirkstoff enthaltendes therapeutisches präparat
EP0910629B1 (de) Verfahren zur Herstellung einer Antidotausgangssubstanz für natürliche und synthetische Thrombininhibitoren
DD251995A5 (de) Verfahren zur Herstellung von Gewebeplasminogenaktivatoren
DD234279A5 (de) Verfahren zur herstellung einer serumunabhaengigen humanzellinie
EP0295432A2 (de) Animales Serum mit verringerter Protease- und Inhibitoraktivität

Legal Events

Date Code Title Description
IF04 In force in the year 2004

Expiry date: 20070606