FR2599625B1 - Procede de production d'activateur du plasminogene biologiquement actif - Google Patents

Procede de production d'activateur du plasminogene biologiquement actif

Info

Publication number
FR2599625B1
FR2599625B1 FR878707905A FR8707905A FR2599625B1 FR 2599625 B1 FR2599625 B1 FR 2599625B1 FR 878707905 A FR878707905 A FR 878707905A FR 8707905 A FR8707905 A FR 8707905A FR 2599625 B1 FR2599625 B1 FR 2599625B1
Authority
FR
France
Prior art keywords
plasminogen activator
biologically active
medium
culture
producing biologically
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
FR878707905A
Other languages
English (en)
Other versions
FR2599625A1 (fr
Inventor
William R Arathoon
Stuart E Builder
Anthony S Lubiniecki
Robert D Van Reis
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genentech Inc
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Publication of FR2599625A1 publication Critical patent/FR2599625A1/fr
Application granted granted Critical
Publication of FR2599625B1 publication Critical patent/FR2599625B1/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
FR878707905A 1986-06-06 1987-06-05 Procede de production d'activateur du plasminogene biologiquement actif Expired - Lifetime FR2599625B1 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US87164286A 1986-06-06 1986-06-06

Publications (2)

Publication Number Publication Date
FR2599625A1 FR2599625A1 (fr) 1987-12-11
FR2599625B1 true FR2599625B1 (fr) 1992-03-13

Family

ID=25357832

Family Applications (1)

Application Number Title Priority Date Filing Date
FR878707905A Expired - Lifetime FR2599625B1 (fr) 1986-06-06 1987-06-05 Procede de production d'activateur du plasminogene biologiquement actif

Country Status (21)

Country Link
US (1) US5053334A (fr)
EP (1) EP0248675B1 (fr)
JP (1) JPS6332484A (fr)
KR (1) KR930002888B1 (fr)
AT (1) ATE75775T1 (fr)
AU (1) AU601984B2 (fr)
DD (1) DD257087A5 (fr)
DE (2) DE3778758D1 (fr)
DK (1) DK175366B1 (fr)
ES (1) ES2032444T3 (fr)
FI (1) FI91886C (fr)
FR (1) FR2599625B1 (fr)
GB (1) GB2191493B (fr)
GR (1) GR3005245T3 (fr)
HU (1) HU205171B (fr)
IE (1) IE60485B1 (fr)
IL (1) IL82746A (fr)
NO (1) NO170595C (fr)
NZ (1) NZ220547A (fr)
PT (1) PT84991B (fr)
ZA (1) ZA874054B (fr)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL82746A (en) * 1986-06-06 1994-10-07 Genentech Inc A process for producing a biologically active tissue plasminogen activator
JP2576200B2 (ja) * 1988-03-09 1997-01-29 味の素株式会社 生理活性タンパク質の製造法
EP0338716A3 (fr) * 1988-04-21 1990-04-11 Berlex Laboratories, Inc. Méthode et appareillage pour la production de protéines non dégradées par des cellules de mammifères
DE3829244A1 (de) * 1988-08-29 1990-03-01 Boehringer Mannheim Gmbh Verfahren zur herstellung von einkettigem t-pa
CA2011548A1 (fr) * 1989-03-07 1990-09-07 Hubertus Stockinger Procede de production de t-pa a deux chaines
GB2249099B (en) * 1990-09-26 1995-05-03 Squibb & Sons Inc Squalene synthetase
US5677184A (en) * 1994-04-19 1997-10-14 Takeda Chemical Industries, Ltd. CHO cells that express human LH-RH receptor
US6077940A (en) * 1997-12-24 2000-06-20 Genentech, Inc. Free solution ligand interaction molecular separation method
WO2003087292A2 (fr) 2002-04-08 2003-10-23 Millenium Biologix Inc. Systeme automatise d'ingenierie des tissus
HUE035109T2 (en) * 2006-07-14 2018-05-02 Patheon Holdings I B V An improved method for cell culture
EP2188371B1 (fr) * 2007-08-09 2017-12-20 Wyeth LLC Utilisation de la perfusion pour améliorer la production d'une culture cellulaire en mode semi-continu dans des bioréacteurs
JP2013517771A (ja) * 2010-01-22 2013-05-20 ロンザ ウォーカーズヴィル，インコーポレーテッド タンジェンシャルフローフィルトレーションを用いた治療用細胞の容量減少および洗浄のための高収率法および装置
US10934519B2 (en) 2011-07-29 2021-03-02 Global Life Sciences Solutions Usa Llc Systems, methods and control laws for cell harvesting
CN110172405A (zh) * 2012-08-20 2019-08-27 泰尔茂比司特公司 浓缩穿过细胞生长腔室循环的流体的组分
US11447745B2 (en) 2017-09-01 2022-09-20 Lonza Walkersville, Inc. End-to-end cell therapy automation
CN113195725A (zh) 2018-12-21 2021-07-30 隆萨沃克斯维尔股份有限公司 病毒载体的自动化生产
CA3123314A1 (fr) 2018-12-21 2020-06-25 Octane Biotech Inc. Carrousel pour unites de production biologique modulaires
CN113227350A (zh) 2018-12-28 2021-08-06 奥克泰生物科技股份有限公司 具有受控环境区的细胞培养和组织工程系统
EP3914690A4 (fr) 2019-02-08 2022-11-09 Lonza Walkersville, Inc. Méthodes et dispositifs de concentration cellulaire destinés à être utilisés dans des bioréacteurs automatisés
AU2022303345A1 (en) 2021-07-02 2024-01-18 Combangio, Inc. Processes for making and using a cellular fibronectin composition

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3904480A (en) * 1972-10-24 1975-09-09 Lilly Co Eli Enhanced production of plasminogen activator
JPS5329985A (en) * 1976-08-27 1978-03-20 Kanegafuchi Chem Ind Co Ltd High concentration culturing of yeast
JPS5571496A (en) * 1978-11-24 1980-05-29 Asahi Chem Ind Co Ltd Preparation of living substance
US4409331A (en) * 1979-03-28 1983-10-11 Damon Corporation Preparation of substances with encapsulated cells
US4232124A (en) * 1979-04-23 1980-11-04 Mann George F Method of producing plasminogen activator
DE3015699C2 (de) * 1979-04-26 1982-07-15 Asahi Kasei Kogyo K.K., Osaka Herstellung eines Plasminogen-Aktivators
JPS5642584A (en) * 1979-09-18 1981-04-20 Asahi Chem Ind Co Ltd Cell cultivation method
US4420398A (en) * 1981-08-13 1983-12-13 American National Red Cross Filteration method for cell produced antiviral substances
IL68561A (en) * 1982-05-05 1991-01-31 Genentech Inc Preparation of polypeptide with human tissue plasminogen activator function,processes for making it,and dna and transformed cell intermediates thereof
JPS5951220A (ja) * 1982-08-02 1984-03-24 Asahi Chem Ind Co Ltd 新規なプラスミノ−ゲン・アクチベ−タ−およびその製法ならびにこれを含有する薬剤
US4537860A (en) * 1982-12-08 1985-08-27 Monsanto Company Static cell culture maintenance system
NZ206699A (en) * 1982-12-30 1989-08-29 Bio Response Inc Process for the production of serum independent cell lines
AU572108B2 (en) * 1983-01-19 1988-05-05 Genentech Inc. Human tpa production using vectors coding for dhfr protein
JPS59121172U (ja) * 1983-02-04 1984-08-15 ミネソタ・マイニング・アンド・マニユフアクチユアリング・コンパニ− ケーブル電線被覆の剥離部分のカバー装置
US4546083A (en) * 1983-04-22 1985-10-08 Stolle Research & Development Corporation Method and device for cell culture growth
DE3479520D1 (en) * 1983-11-21 1989-09-28 Ciba Geigy Ag Synthesis of tissue plasminogen activator(tpa) by yeast
US4740461A (en) * 1983-12-27 1988-04-26 Genetics Institute, Inc. Vectors and methods for transformation of eucaryotic cells
US4888115A (en) * 1983-12-29 1989-12-19 Cuno, Incorporated Cross-flow filtration
US4757005A (en) * 1984-04-19 1988-07-12 Miles Inc. Method and cell line for obtaining plasminogen activators
EP0215923B1 (fr) * 1985-03-22 1990-12-27 Chiron Corporation EXPRESSION DE tPA DANS DE CELLULES DE MAMMIFERES
AU593264B2 (en) * 1985-07-10 1990-02-08 Kanegafuchi Kagaku Kogyo Kabushiki Kaisha Chromosomal DNA sequence, expression vector for human tissue plasminogen activating factor, cultured cells transfected with same and method of producing said activating factor
AU606582B2 (en) * 1985-09-06 1991-02-14 Codon Methods for the recovery of tissue plasminogen activator
JPH07110232B2 (ja) * 1985-10-14 1995-11-29 株式会社日立製作所 遺伝子組換え大腸菌の遺伝子生産物を採取する方法および装置
IL82746A (en) * 1986-06-06 1994-10-07 Genentech Inc A process for producing a biologically active tissue plasminogen activator

Also Published As

Publication number Publication date
ES2032444T3 (es) 1993-02-16
HU205171B (en) 1992-03-30
FI872526A0 (fi) 1987-06-05
AU601984B2 (en) 1990-09-27
IL82746A0 (en) 1987-12-20
FR2599625A1 (fr) 1987-12-11
US5053334A (en) 1991-10-01
ZA874054B (en) 1989-02-22
NO170595C (no) 1992-11-04
NZ220547A (en) 1990-01-29
AU7387287A (en) 1987-12-10
DE3718939C2 (de) 1998-09-17
JPH0574346B2 (fr) 1993-10-18
DE3778758D1 (de) 1992-06-11
IL82746A (en) 1994-10-07
HUT43875A (en) 1987-12-28
IE871504L (en) 1987-12-06
FI91886B (fi) 1994-05-13
EP0248675A1 (fr) 1987-12-09
DD257087A5 (de) 1988-06-01
KR930002888B1 (ko) 1993-04-12
ATE75775T1 (de) 1992-05-15
EP0248675B1 (fr) 1992-05-06
DK175366B1 (da) 2004-09-13
DK288187D0 (da) 1987-06-04
GB2191493A (en) 1987-12-16
GB8713267D0 (en) 1987-07-08
NO872395D0 (no) 1987-06-05
GB2191493B (en) 1991-02-27
DE3718939A1 (de) 1987-12-17
FI91886C (fi) 1994-08-25
NO170595B (no) 1992-07-27
JPS6332484A (ja) 1988-02-12
DK288187A (da) 1987-12-07
KR880000590A (ko) 1988-03-28
GR3005245T3 (fr) 1993-05-24
PT84991B (pt) 1990-03-08
FI872526A (fi) 1987-12-07
NO872395L (no) 1987-12-07
PT84991A (en) 1987-07-01
IE60485B1 (en) 1994-07-27

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