CN1863914A - 新的草甘膦-n-乙酰转移酶(gat)基因 - Google Patents
新的草甘膦-n-乙酰转移酶(gat)基因 Download PDFInfo
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Abstract
提供了新的蛋白质,包括能够催化草甘膦乙酰化的蛋白质和其他结构上相关的蛋白质。还提供了能够编码这些蛋白质的新的多核苷酸、包括一种或多种这些新蛋白质和/或多核苷酸的组合物、包含这些新的化合物的重组细胞和转基因植物、涉及新化合物的多样化方法以及使用该化合物的方法。在此提供的新方法和化合物的一些被用于赋予生物体例如植物对草甘膦的抗性。
Description
依据37 C.F.R.1.71(E)的版权通告
本发明文件的一部分分开内容包括受到版权保护的材料。版权所有者不反对任何人复制此专利文件或者专利公开,对于专利商标局的专利文件或者记录而言,这是显而易见的,但是无论如何版权所有者保留所用的版权。
发明背景
将编码适当的除草剂代谢酶类的基因转移到农作物中,可以赋予农作物对特定除草剂的选择性。在某些情况下,这些酶类以及编码它们的核酸来源于植物。在其他情况下,它们来源于其他生物体,例如微生物。参见,例如,Padgette等人(1996)“New weed controlopportunities:Development of soybeans with a Round UPReadyTMgene”和Vasil(1996)“Phosphinothricin-resistant crops”,都出自Herbicide-Resistant公司,Duke编辑(CRC出版社,伯克莱屯,佛罗里达州)第54-84页和第85-91页。实际上,已经从各种生物来源构建了表达各种除草剂耐受性/代谢基因的转基因植物,例如,乙酰羟基酸合酶已经可以使表达这种酶的植物对多种类型的除草剂产生抗性,已经被导入许多植物中(参见。例如Hattori等人(1995)Mol.Gen.genet.246:419)。赋予对除草剂的抗性的其他基因包括:编码大鼠细胞色素P4507A1和酵母NADPH-细胞色素P450氧化还原酶嵌合蛋白的基因(Shiota等人(1994)Plant Physiol.106:17)、谷胱甘肽还原酶基因和超氧化物歧化酶基因(Aono等人(1995)PlantCell Physiol 36:1687)以及各种磷酸转移酶的基因(Datta等人(1992)Plant Mol.Biol.20:619)。
在这方面,作为许多研究的主题的一种除草剂是N-膦酰基甲基甘氨酸,它通常被称作为草甘膦。草甘膦是世界上最畅销的除草剂,2003年的销售量计划高达50亿美元。它是一种广谱除草剂,可以杀死阔叶植物和草本植物。在转基因植物中产生草甘膦抗性的一种成功的商业模式是通过导入被修饰的农杆菌属CP4 5-烯醇丙酮酰莽草酸-3-磷酸酯合酶(下文被称作为EPSP合酶或者EPSPS)基因来实现。转基因被靶向叶绿体,存在草甘膦时,转基因在叶绿体中能够连续地将磷酸烯醇式丙酮酸(PEP)和莽草酸-3-磷酸酯合成为EPSP。相反,草甘膦会抑制天然的EPSP合酶。如果没有转基因,喷洒过草甘膦的植物会因为EPSP合酶被抑制而很快死亡,EPSP合酶会阻断芳香性氨基酸、激素和维生素生物合成所需的下游途径。例如,Monsanto已经研制了食品名为“Round UP ReadyTM”的CP4草甘膦抗性的大豆转基因植物。
这种情况下,草甘膦被降解的主要机制是通过土壤微生物代谢进行。已经确定土壤中的草甘膦的主要代谢产物是氨基甲基膦酸(AMPA),它最终被转化为铵盐、磷酸和二氧化碳。图8中显示了描述所提出的在土壤中通过AMPA路径降解草甘膦的代谢过程。如图9中所描述,通过某些土壤细菌分解草甘膦的其他代谢途径,肌氨酸途径,通过首先断裂
键而产生无机磷酸和肌氨酸。
另一种成功的除草剂/转基因农作物是草铵膦(膦丝菌素)和例如Aventis销售的Liberty LinkTM。草铵膦也是一种广谱除草剂。其靶点是叶绿体的谷氨酸合酶。抗性植物携带来自吸水链霉菌的bar基因,并且通过bar的N-乙酰化活性获得抗性,bar可修饰并解毒谷氨酸。
PCT申请WO 00/29596中报道了一种能够使AMPA的伯胺发生乙酰化的酶。但是没有描述这种酶能够使具有仲胺的化合物(例如,草甘膦)发生乙酰化。
如上所述,虽然有各种除草剂抗性的策略可被利用,但是其他方法将具有巨大的商业价值。本发明提供赋予除草剂以耐受性的新多核苷酸和多肽,以及在阅读以下公开内容时,许多其他优点将会变得显而易见的。
发明概述
本发明提供方法和试剂,用于通过如下描述的一种或多种实施方案,使一种生物,例如植物,对草甘膦产生抗性。
本发明的一个实施方案提供新的多肽,在此被称作为草甘膦-N-乙酰转移酶(“GAT”)多肽。GAT多肽被表征为,当GAT多肽相互比对时,就序列相似性而言,它们的结构是彼此相似的。本发明的GAT多肽具有草甘膦-N-乙酰转移酶活性,即催化草甘膦乙酰化的活性。这些GAT多肽将乙酰基由乙酰CoA转移到草甘膦的N末端上。此外,一些GAT多肽将丙酰CoA的丙酰基转移到草甘膦的N末端上。一些GAT多肽还能够催化草甘膦类似物和/或草甘膦代谢产物的乙酰化,如氨基甲基膦酸。示例性的GAT多肽对应于SEQ ID NO:568、569、570、571、572、573、574、575、576、577、578、579、580、581、582、583、584、585、586、587、588、589、590、591、592、593、594、595、596、597、598、599、600、601、602、603、604、605、606、607、608、609、610、611、612、613、614、615、616、617、618、619、621、623、625、627、629、631、633、635、637、639、641、643、645、647、649、651、653、655、657、659、661、663、665、667、669、671、673、675、677、679、681、683、685、687、689、691、693、695、697、699、701、703、705、707、709、711、713、715、717、719、721、723、725、727、729、731、733、735、737、739、741、743、745、747、749、751、753、755、757、759、761、763、765、767、769、771、773、775、777、779、781、783、785、787、789、791、793、795、797、799、801、803、805、807、809、811、813、815、817、819、821、823、825、833、835、837、839、841、843、845、847、849、851、853、855、857、859、861、863、865、867、869、871、873、875、877、879、881、883、885、887、889、891、893、895、897、899、901、903、905、907、909、911、913、915、917、919、921、923、925、927、929、931、946、948、950、953、954、955、956、957、958、959、960、961、962、963、964、965、966、967、968、969、970、971和972。
还提供在此被称作为GAT多核苷酸的新多核苷酸,例如SEQ ID NO:516、517、518、519、520、521、522、523、524、525、526、527、528、529、530、531、532、533、534、535、536、537、538、539、540、541、542、543、544、545、546、547、548、549、550、551、552、553、554、555、556、557、558、559、560、561、562、563、564、565、566、567、620、622、624、626、628、630、632、634、636、638、640、642、644、646、648、650、652、654、656、658、660、662、664、666、668、670、672、674、676、678、680、682、684、686、688、690、692、694、696、698、700、702、704、706、708、710、712、714、716、718、720、722、724、726、728、730、732、734、736、738、740、742、744、746、748、750、752、754、756、758、760、762、764、768、770、772、774、776、778、780、782、784、786、788、790、792、794、796、798、800、802、804、806、808、810、812、814、816、818、820、822、824、832、834、836、838、840、842、844、846、848、850、852、854、856、858、860、862、864、866、868、870、872、874、876、878、880、882、884、886、888、890、892、894、896、898、900、902、904、906、908、910、912、914、916、918、920、922、924、926、928、930、932、933、934、935、936、937、938、939、940、941、942、943、944、945、947、949、951和952。GAT多核苷酸被表征为具有编码GAT多肽的能力。在本发明的一些实施方案中,GAT多核苷酸被工程化,用同义密码子替代一个或多个亲本密码子,以更好地进行植物表达,相对于亲本密码子,这些同义密码子在植物中被优先使用。在其他实施方案中,引入编码N-末端叶绿体转运肽的核苷酸序列来修饰GAT多核苷酸。在其他实施方案中,通过将一个或多个含有G+C的密码子(例如GCG或GCT)插入在紧接和邻近Met密码子的下游,来修饰GAT多核苷酸。
GAT多肽、GAT多核苷酸和草甘膦-N-乙酰转移酶活性将在下文被更加详细地描述。本发明还包括在此描述的GAT多肽和GAT多核苷酸的某些片段。
本发明包括在此描述的多肽和多核苷酸的非天然变体,其中一个或多个编码多肽的氨基酸被突变。
在某些优选的实施方案中,本发明的GAT多肽被如下表征。当采用B1OSUM62矩阵,存在一个缺口罚11分,和延伸一个缺口罚1分,最佳地与参比氨基酸序列比对时,获得至少为460的相似性分值,参比氨基酸序列选自由SEQ ID NO:300、445或457组成的组,一个或多个如下位置遵从如下限制:(i)在位置18和38上,为Z5氨基酸残基;(ii)在位置62上,为Z1氨基酸残基;(iii)在位置124上,为Z6氨基酸残基;和(iv)在位置144上,为Z2氨基酸残基,其中:Z1是选自由A、I、L、M和V组成的组的氨基酸残基;Z2是选自由F、W和Y组成的组的氨基酸残基;Z5是选自由D和E组成的组的氨基酸残基;和Z6是选自由C、G和P组成的组的氨基酸残基。
本发明还提供分离的和重组的多肽,包含选自由如下组成的组的氨基酸序列:(a)与SEQ ID NO:577至少98%相同的氨基酸序列;(b)与SEQ ID NO:578至少97%相同的氨基酸序列;(c)与SEQ ID NO:621至少97%相同的氨基酸序列;(d)与SEQ ID NO:579至少98%相同的氨基酸序列;(e)与SEQ ID NO:602至少98%相同的氨基酸序列;(f)与SEQ ID NO:697至少95%相同的氨基酸序列;(g)与SEQ ID NO:712至少96%相同的氨基酸序列;(h)与SEQ ID NO:613至少97%相同的氨基酸序列;(i)与SEQ ID NO:677至少89%相同的氨基酸序列;(j)与SEQ ID NO:584至少95%相同的氨基酸序列;(k)与SEQ ID NO:707至少98%相同的氨基酸序列;(l)与SEQ ID NO:616至少98%相同的氨基酸序列;(m)与SEQ ID NO:612至少96%相同的氨基酸序列;和(n)与SEQ ID NO:590至少98%相同的氨基酸序列。
本发明还提供分离的和重组的多肽,其包含选自由如下组成的组的氨基酸序列:(a)与SEQ ID NO:919的位置2-146至少96%相同的氨基酸序列(例如SEQ ID NO:917、919、921、923、925、927、833、835、839、843、845、859、863、873、877、891、895、901、905、907、913、915或者950);(b)与SEQ ID NO:929的位置2-146至少97%相同的氨基酸序列(例如,SEQ ID NO:929、931、835、843、849或者867);(c)与SEQ ID NO:847的位置2-146至少98%相同的氨基酸序列(例如,SEQ ID NO:845或847);(d)与SEQ ID NO:851位置2-146至少98%相同的氨基酸序列;(e)与SEQ ID NO:853的位置2-146至少98%相同的氨基酸序列;(f)与SEQ ID NO:855的位置2-146至少98%相同的氨基酸序列(例如SEQ ID NO:835或855);(g)与SEQ ID NO:857的位置2-146至少98%相同的氨基酸序列;(h)与SEQ ID NO:861的位置2-146至少98%相同的氨基酸序列(例如,SEQ ID NO:839、861或者883);(i)与SEQ ID NO:871的位置2-146至少98%相同的氨基酸序列;(j)与SEQ ID NO:875的位置2-146至少98%相同的氨基酸序列;(k)与SEQ ID NO:881的位置2-146至少98%相同的氨基酸序列;(l)与SEQ ID NO:885的位置2-146至少98%相同的氨基酸序列(例如,SEQ ID NO:845或885);(m)与SEQ ID NO:887的位置2-146至少98%相同的氨基酸序列;(n)与SEQ ID NO:889的位置2-146至少98%相同的氨基酸序列(例如SEQ ID NO:863、889、891或903);(o)与SEQ ID NO:893的位置2-146少98%相同的氨基酸序列;(p)与SEQ ID NO:897的位置2-146至少98%相同的氨基酸序列;(q)与SEQ ID NO:899的位置2-146至少98%相同的氨基酸序列;(r)与SEQ ID NO:909的位置2-146至少98%相同的氨基酸序列(例如SEQID NO:883或909);(s)与SEQ ID NO:911的位置2-146少98%相同的氨基酸序列;(t)与SEQ ID NO:837的位置2-146至少99%相同的氨基酸序列;(u)与SEQ ID NO:841的位置2-146至少99%相同的氨基酸序列;(v)与SEQ ID NO:865的位置2-146至少99%相同的氨基酸序列;(w)与SEQ ID NO:869的位置2-146至少99%相同的氨基酸序列;和(x)与SEQ ID NO:879的位置2-146至少99%相同的氨基酸序列。在本发明的一些实施方案中,多肽的氨基酸序列在对应于参比氨基酸序列的位置2的氨基酸的N末端侧包含Met、Met-Ala或Met-Ala-Ala。
本发明还提供分离的和重组的多肽,其包含与SEQ ID NO:929的位置2-146至少95%相同的氨基酸序列,其在对应于SEQ ID NO:929位置33上包含Gly或者Asn残基(例如,SEQ ID NO:837、849、893、897、905、921、927、929或者931)。在本发明的一些实施方案中,多肽的氨基酸序列在对应于参比氨基酸序列的位置2的氨基酸的N末端侧包含Met、Met-Ala或者Met-Ala-Ala。
本发明还提供包含本发明的多核苷酸的核酸构建体。该构建体可以是载体,例如植物转化载体。在某些方面,本发明的载体包含T-DNA序列。该构建体任选地包括可操作地连接到GAT多核苷酸上的调控序列(例如启动子),其中启动子相对于多核苷酸来说是异源的,有效地引起充分表达所编码的多肽,以提高用核酸构建体转化的植物细胞的草甘膦耐受性。
在本发明的某些方面,例如在植物、细菌、放射菌、酵母、藻类或其他真菌中,GAT多核苷酸作为可选择的标记起作用。例如,可以根据其在存在草甘膦时生长的能力来选择用包含GAT多核苷酸的可选择标记的载体转化的生物体。GAT标记基因被用于选择或筛选表达该基因的被转化细胞。
本发明还提供具有多重特性的载体,即编码GAT多肽的载体,并且该载体包括编码第二种多肽的第二条多核苷酸序列,该第二种多肽可以使以有效水平表达第二种多肽的细胞或生物体具有一种可检测的表型特性。可检测的表型特性可以作为可检测的标记,例如,通过提供除草剂抗性、害虫抗性或提供某些类型的可视标记。
在一些实施方案中,本发明提供一种包含两种或多种本发明的多核苷酸的组合物。优选地,GAT多核苷酸编码具有不同动力学参数的GAT多肽,即具有较低的Km的GAT变体可以与具有较高的k催化的变体组合。在另一个实施方案中,不同的GAT多核苷酸与叶绿体转运序列或其他信号序列连接,从而在不同细胞腔室、细胞器中表达GAT多肽或者分泌一种或多种GAT多肽。
因此,含有两种或多种GAT多核苷酸或者所编码的多肽的组合物是本发明的一个特征。在一些情况下,这些组合物是含有例如至少3种或多种这种核酸的核酸文库。通过用限制性核酸内切酶、DNA酶或者RNA酶消化本发明的核酸,或者另外地切断核酸,例如机械剪接、化学断裂等而生产的组合物,也是本发明的一种特征,将本发明的核酸与脱氧核糖核酸三磷酸和核酸聚合酶,例如热稳定的核酸聚合酶,一起温育而制得的组合物也是本发明的一种特征。
用本发明的载体转化的或者另外地掺入了本发明的核酸的细胞是本发明的一个方面。在一个优选实施方案中,细胞表达由本发明的核酸编码的多肽。
在一些实施方案中,掺入了本发明的核酸的细胞是植物细胞。掺入了本发明的核酸的转基因植物、转基因植物细胞以及转基因植物外植体也是本发明的一个特征。在一些实施方案中,转基因植物、转基因植物细胞,或者转基因植物外植体表达本发明的核酸编码的具有草甘膦-N-乙酰转移酶活性的外源多肽。本发明还提供由本发明的转基因植物生产的转基因种子。
本发明还提供具有增强的对草甘膦的抗性的转基因植物、转基因植物细胞、转基因植物外植体,或者转基因种子,这是由于表达了具有草甘膦-N-乙酰转移酶活性的多肽和通过其他机制赋予对草甘膦的耐受性的多肽,例如耐受草甘膦的5-烯醇丙酮酰莽草酸-3-磷酸酯合酶和/或耐受草甘膦的草甘膦氧化还原酶。在另一个实施方案中,本发明提供具有增强的对草甘膦的耐受性以及对其他除草剂耐受的转基因植物或者转基因植物外植体,这是由于表达了具有草甘膦-N-乙酰转移酶活性的多肽,通过其他机制赋予对草甘膦的耐受性的多肽,例如耐受草甘膦的5-烯醇丙酮酰莽草酸-3-磷酸酯合酶和/或耐受草甘膦的草甘膦氧化还原酶以及赋予对其他除草剂的耐受性的多肽,例如突变的羟苯基丙酮酸二氧化酶、耐受磺胺的乙酰乳酸合酶、耐受磺胺的乙酰羟基酸合酶、耐受咪唑啉酮的乙酰乳酸的合酶、耐受咪唑啉酮的乙酰羟基酸合酶、膦丝菌素乙酰转移酶和突变的原卟啉原氧化酶。
本发明还提供具有增强的对草甘膦的耐受性以及耐受其他除草剂的转基因植物、转基因植物细胞、转基因植物外植体或者转基因种子,这是由于表达了具有草甘膦-N-乙酰转移酶活性的多肽以及赋予对其他除草剂的抗性的多肽,例如突变的羟苯基丙酮酸二氧化酶、耐受磺胺的乙酰乳酸合酶、耐受磺胺的乙酰羟酸合酶、耐受咪唑啉酮的乙酰乳酸的合酶、耐受咪唑啉酮的乙酰羟酸合酶、膦丝菌素乙酰转移酶和突变的原卟啉原氧化酶。本发明还提供具有增强的对草甘膦的耐受性以及其他的期望特性的转基因植物、转基因植物细胞、转基因植物外植体或者转基因种子,其他期望特性是通过一种或多种其他转基因来赋予的。
生产本发明的多肽的方法是本发明的一个特征,通过将编码多肽的核酸导入细胞,然后进行表达并任选地从细胞或者培养基中回收。在优选实施方案中,表达本发明的多肽的细胞是转基因植物细胞。
提高本发明的多肽在植物或植物细胞中的表达水平的方法也是本发明的一个特征,这是通过在多肽编码序列中,在邻近于起始甲硫氨酸ATG密码子和在该密码子的下游插入一个或多个富含G/C的密码子(GCG或GCT),和/或将在多肽编码序列中的一个或多个在植物中较少被利用的密码子取代为在植物中较常使用的编码相同氨基酸的密码子,并且将被修饰的编码序列插入到植物或者植物细胞中,并表达被修饰的修饰序列而完成的。
特异地被与来源于下列的抗原反应:SEQ ID NO:568、569、570、571、572、573、574、575、576、577、578、579、580、581、582、583、584、585、586、587、588、589、590、591、592、593、594、595、596、597、598、599、600、601、602、603、604、605、606、607、608、609、610、611、612、613、614、615、616、617、618、619、621、623、625、627、629、631、633、635、637、639、641、643、645、647、649、651、653、655、657、659、661、663、665、667、669、671、673、675、677、679、681、683、685、687、689、691、693、695、697、699、701、703、705、707、709、711、713、715、717、719、721、723、725、727、729、731、733、735、737、739、741、743、745、747、749、751、753、755、757、759、761、763、765、767、769、771、773、775、777、779、781、783、785、787、789、791、793、795、797、799、801、803、805、807、809、811、813、815、817、819、821、823、825、833、835、837、839、841、843、845、847、849、851、853、855、857、859、861、863、865、867、869、871、873、875、877、879、881、883、885、887、889、891、893、895、897、899、901、903、905、907、909、911、913、915、917、919、921、923、925、927、929、931、946、948、950、953、954、955、956、957、958、959、960、961、962、963、964、965、966、967、968、969、970、971和972,但是不与天然存在的相关序列反应的多克隆抗血清结合的多肽,例如由GenBank登录号CAA70664的那些序列的子序列表示的肽,以及由施用来源于下列的抗原中的一种或多种而生产的抗体:SEQ ID NO:568、569、570、571、572、573、574、575、576、577、578、579、580、581、582、583、584、585、586、587、588、589、590、591、592、593、594、595、596、597、598、599、600、601、602、603、604、605、606、607、608、609、610、611、612、613、614、615、616、617、618、619、621、623、625、627、629、631、633、635、637、639、641、643、645、647、649、651、653、655、657、659、661、663、665、667、669、671、673、675、677、679、681、683、685、687、689、691、693、695、697、699、701、703、705、707、709、711、713、715、717、719、721、723、725、727、729、731、733、735、737、739、741、743、745、747、749、751、753、755、757、759、761、763、765、767、769、771、773、775、777、779、781、783、785、787、789、791、793、795、797、799、801、803、805、807、809、811、813、815、817、819、821、823、825、833、835、837、839、841、843、845、847、849、851、853、855、857、859、861、863、865、867、869、871、873、875、877、879、881、883、885、887、889、891、893、895、897、899、901、903、905、907、909、911、913、915、917、919、921、923、925、927、929、931、946、948、950、953、954、955、956、957、958、959、960、961、962、963、964、965、966、967、968、969、970、971和972,和/或特异地结合到这种抗原上而不特异地结合到对应于GenBank登录号CAA70664的那些天然存在的多肽上的抗体,都是本发明的特征。
本发明的另一个方面涉及,通过体外或体内重组或突变本发明的核酸使多核苷酸多样化,来生成新的GAT多核苷酸和多肽的方法。在一个实施方案中,重组产生至少一种重组的GAT多核苷酸文库。如此产生的文库是本发明的实施方案,包含该文库的细胞也是本发明的实施方案。此外,通过突变本发明的核酸来生产被修饰的GAT多核苷酸的方法是本发明的实施方案。通过本发明的方法生产的重组和突变的GAT多核苷酸和多肽也是本发明的实施方案。
在本发明的某些方面,通过采用递推重组来实现多样化,可以在体外、在体内、在计算机芯片上或者它们的组合中完成。下文中被更加详细地描述的多样化方法的一些例子是家族改组方法和合成改组方法。本发明提供用于生产草甘膦抗性的转基因植物或植物细胞的方法,包括用编码草甘膦-N-乙酰转移酶的多核苷酸转化植物或植物细胞,并且任选地从被转化的植物细胞中再生转基因植物。在一些方面,多核苷酸是GAT多核苷酸,任选地GAT多核苷酸来源于细菌来源。在本发明的一些方面,该方法可以包括使被转化的植物或植物细胞生长在抑制同一物种的野生型植物生长而不抑制被转化的植物生长的草甘膦浓度中。该方法包括使被转化的植物或植物细胞或植物或植物的后代生长在浓度升高的草甘膦中和/或在对同一物种野生型植物或植物细胞有致死作用的草甘膦中。通过这种方法制备的耐受草甘膦的转基因植物可以被繁殖,例如通过将其与第二种植物杂交,使得至少某些杂交后代具有草甘膦耐受性。
本发明还提供用于选择性地控制具有农作物的田间的杂草的方法,包括在田间种植耐受草甘膦的农作物种子或者植物,它们转化了编码草甘膦N-乙酰转移酶的基因,往田间的农作物和杂草施用足量的草甘膦,以控制杂草而不会显著地影响农作物。
本发明还提供控制田间杂草和防止在含有农作物的田间出现草甘膦抗性杂草的方法,包括在田间种植耐受草甘膦的农作物种子或者植物,这些种子或植物转化了编码草甘膦-N-乙酰转移酶的基因和编码通过其他机制赋予对草甘膦的耐受性的多肽的基因,例如耐受草甘膦的5-烯醇丙酮酰莽草酸-3-磷酸酯合酶和/或耐受草甘膦的草甘膦氧化还原酶,给田间的农作物和杂草施用足量的草甘膦,以控制杂草而不显著影响农作物。
在另一个实施方案中,本发明提供用于控制田间杂草和防止在含有农作物的田间出现除草剂抗性的杂草的方法,其包括在田间种植耐受草甘膦的农作物种子或植物,这些种子或植物转化了编码草甘膦-N-乙酰转移酶的基因和编码通过其他机制赋予对草甘膦的耐受性的多肽的基因,例如耐受草甘膦的5-烯醇丙酮酰莽草酸-3-磷酸酯合酶和/或耐受草甘膦的草甘膦氧化还原酶,以及编码赋予对其他除草剂耐受性的多肽的基因,例如突变的羟苯基丙酮酸二氧化酶、耐受磺胺的乙酰乳酸合酶、耐受磺胺的乙酰羟酸合酶、耐受咪唑啉酮的乙酰乳酸的合酶、耐受咪唑啉酮的乙酰羟酸合酶、膦丝菌素乙酰转移酶和突变的原卟啉原氧化酶。给田间的农作物和杂草施用足量的草甘膦和其他除草剂,例如羟苯基丙酮酸二氧化酶抑制剂、磺胺、咪唑啉酮、双丙氨膦(bialaphos)、膦丝菌素、唑啶草酮(azafenidin)、氟丙嘧草酯(butafenacil)、磺酸盐、草铵膦(glufosinate)和原卟啉原氧化酶(protox)抑制剂,以控制杂草而不显著影响农作物。
本发明还提供用于控制田间杂草和防止在含有农作物的田间出现除草剂抗性杂草的方法,其包括在田间种植耐受草甘膦的农作物种子或植物,这些种子或植物转化了编码草甘膦-N-乙酰转移酶的基因和编码赋予对其他除草剂抗性的多肽的基因,例如例如突变的羟苯基丙酮酸二氧化酶、耐受磺胺的乙酰乳酸合酶、耐受磺胺的乙酰羟酸合酶、耐受咪唑啉酮的乙酰乳酸的合酶、耐受咪唑啉酮的乙酰羟酸合酶、膦丝菌素乙酰转移酶和突变的原卟啉原氧化酶,给田间的农作物和杂草施用足量的草甘膦和其他除草剂,例如羟苯基丙酮酸二氧化酶抑制剂、磺胺、咪唑啉酮、双丙氨膦、膦丝菌素,唑啶草酮、氟丙嘧草酯、磺酸盐、草铵膦和原卟啉原氧化酶抑制剂,以控制杂草而不显著影响农作物。
本发明还提供用于生产经过遗传转化而耐受草甘膦的植物,包括在植物细胞的基因组中插入重组的双链的DNA分子,包含:(i)在植物细胞中起作用以生成RNA序列的启动子;(ii)产生编码GAT的RNA序列的结构性DNA序列;和(iii)3′非翻译区,其在植物细胞中起作用,以将一段多聚腺苷酸核苷酸添加到RNA序列的3’端;其中启动子相对于结构性DNA序列是异源的,并且适合于引起充足地表达被编码的多肽,以提高用DNA分子转化的植物细胞对草甘膦的耐受性;获得转化的植物细胞;和从被转化的植物细胞中再生对草甘膦的耐受性增强的遗传转化的植物。
本发明还提供用于生产农作物的方法,包括种植耐受草甘膦的农作物,该植物耐受草甘膦是由于用编码草甘膦N-乙酰转移酶的基因进行了转化,在某种条件下,使得农作物植物生产一种农作物;和从该农作物植物收获农作物。这些方法通常包括将草甘膦以足以控制杂草的浓度应用于农作物植物。示例性的农作物植物包括棉花、玉米和大豆。
本发明还提供计算机、计算机可读介质和集成系统,包括由包括对应于SEQ ID NO:1-10、16、48、190、193、196、202、205、268、300、442、445、448、454、457、515-830和832-972的字符串的序列记录组成的数据库。这种集成系统任选地包括一套或多套装置,用于在对应于SEQ ID NO:1-10、16、48、190、193、196、202、205、268、300、442、445、448、454、457、515-830和832-972的任何一条或多条字符串之间和/或与任何其他核酸或氨基酸序列之间进行选择、比对、翻译、反向翻译或者视察。
附图简介
附图1描述由草甘膦-N-乙酰转移酶(“GAT”)催化草甘膦的N-乙酰化。
附图2图解说明质谱检测由示例性的表达天然的GAT活性的杆菌培养物生产的N-乙酰草甘膦。
附图3是图解说明从不同的细菌菌株中分离的GAT序列与来自枯草芽孢杆菌的yitI之间的相对同一性的表格。
附图4是用于表达和纯化来自大肠杆菌培养物的GAT酶的质粒pMAXY2120的图谱。
附图5是显示在典型的GAT酶反应混合物中,N-乙酰草甘膦产量随着时间而升高的质谱结果。
附图6是GAT酶的动力学数据的图形,从该图形中计算出的草甘膦的KM为2.9mM。
附图7是从附图6中获得的动力学数据的图形,从该图形中计算出的乙酰CoA的KM为2μM。
附图8是描述在土壤中通过AMPA路径降解草甘膦的图解。
附图9是描述草甘膦降解的肌氨酸路径的图解。
附图10是B1OSUM62基质。
附图11是质粒pMAXY2190的图谱。
附图12描述具有可选择的标记的T-DNA构建体。
附图13描述具有gat可选择标记的酵母表达载体。
附图14图解说明抽穗时草甘膦对植物的高度的影响。
附图15A和15B分别提供如实施例18中所述,在缺乏所添加的 (无阴影栏)或者存在20mM
(阴影栏)时分析的各种GAT酶的动力学参数Km和k催化/Km的比较,如果存在,误差栏表示多个分析的标准方差。
附图16A、16B和16C提供了本发明的各种GAT酶(无阴影栏)的动力学参数Km、k催化和k催化/Km与本发明的一些进一步进化的GAT酶(阴影栏)的动力学参数之间的比较,如实施例19中所描述。如果存在,误差栏表示多个分析的标准方差。
附图17描述了在各种温度下温育后,仍保留GAT活性,如实施例16中所描述。
附图18描述了pH对K催化和KM的作用,如实施例30所描述。
发明详描
本发明涉及一类新的具有N-乙酰转移酶活性的酶。一个方面,本发明涉及一类新的能够乙酰化草甘膦和草甘膦类似物的酶,例如具有草甘膦-N-乙酰转移酶(“GAT”)活性的酶。这种酶的特征是具有将化合物的仲胺乙酰化的能力。在本发明的一些方面,该化合物是除草剂,例如草甘膦,如附图1示例性地图解说明。该化合物还可以是草甘膦类似物或者草甘膦降解的代谢产物,例如氨基甲基膦酸。尽管草甘膦的乙酰化在草甘膦代谢分解的代谢路径中是关键的催化步骤,但是以前没有描述过由天然的、分离的或重组的酶催化的草甘膦的乙酰化。因此,本发明的核酸和多肽提供新的用于工程化除草剂抗性的生物化学路径。
一个方面,本发明提供编码GAT多肽的新基因。对应于天然存在的多核苷酸的分离的和重组的GAT多核苷酸以及重组和工程化的例如多样化的GAT多核苷酸是本发明的一个特征。GAT多核苷酸以SEQ IDNO:516、517、518、519、520、521、522、523、524、525、526、527、528、529、530、531、532、533、534、535、536、537、538、539、540、541、542、543、544、545、546、547、548、549、550、551、552、553、554、555、556、557、558、559、560、561、562、563、564、565、566、567、620、622、624、626、628、630、632、634、636、638、640、642、644、646、648、650、652、654、656、658、660、662、664、666、668、670、672、674、676、678、680、682、684、686、688、690、692、694、696、698、700、702、704、706、708、710、712、714、716、718、720、722、724、726、728、730、732、734、736、738、740、742、744、746、748、750、752、754、756、758、760、762、764、768、770、772、774、776、778、780、782、784、786、788、790、792、794、796、798、800、802、804、806、808、810、812、814、816、818、820、822、824、832、834、836、838、840、842、844、846、848、850、852、854、856、858、860、862、864、866、868、870、872、874、876、878、880、882、884、886、888、890、892、894、896、898、900、902、904、906、908、910、912、914、916、918、920、922、924、926、928、930、932、933、934、935、936、937、938、939、940、941、942、943、944、945、947、949、951和952为例。特异的GAT多核苷酸和多肽序列被提供作为例子,用于阐述本发明,而不将在此所描述和/或要求保护的GAT多核苷酸和多肽限制在这一范围内。
本发明还提供用于生成和选择多样化文库的方法,以生成其他GAT多核苷酸,包括编码具有改良和/或增强特性,例如被改变的草甘膦的Km、升高的催化速度、增强的稳定性等的GAT多肽的多核苷酸,根据对构成文库的多核苷酸进行选择,以获得在此描述的新的和改良的活性。这种多核苷酸被特别有利地用于生产草甘膦抗性的转基因植物。
本发明的GAT多肽具有新的催化特性。特别地,在本发明之前,酶催化的合成除草剂草甘膦的乙酰化还未被认识到。因此,在此描述的多肽,例如以SEQ ID N0:568、569、570、571、572、573、574、575、576、577、578、579、580、581、582、583、584、585、586、587、588、589、590、591、592、593、594、595、596、597、598、599、600、601、602、603、604、605、606、607、608、609、610、611、612、613、614、615、616、617、618、619、621、623、625、627、629、631、633、635、637、639、641、643、645、647、649、651、653、655、657、659、661、663、665、667、669、671、673、675、677、679、681、683、685、687、689、691、693、695、697、699、701、703、705、707、709、711、713、715、717、719、721、723、725、727、729、731、733、735、737、739、741、743、745、747、749、751、753、755、757、759、761、763、765、767、769、771、773、775、777、779、781、783、785、787、789、791、793、795、797、799、801、803、805、807、809、811、813、815、817、819、821、823、825、833、835、837、839、841、843、845、847、849、851、853、855、857、859、861、863、865、867、869、871、873、875、877、879、881、883、885、887、889、891、893、895、897、899、901、903、905、907、909、911、913、915、917、919、921、923、925、927、929、931、946、948、950、953、954、955、956、957、958、959、960、961、962、963、964、965、966、967、968、969、970、971和972为例,详细说明了脱毒草甘膦的新生物化学路径,该路径在体内如植物中起作用。
因此,本发明的核酸和多肽在通过新核酸、多肽和生物化学路径在转基因植物中工程化除草剂选择性来产生草甘膦抗性植物中具有重要的用途。
定义
在详细描述本发明之前,应当理解,本发明不限于特定的组合物或生物系统,当然,它们是可改变的。还应当理解,在此所用的术语仅用于描述特定的实施方案,而不用于限制。除非内容另外清楚地指出,如在本说明书和附随的权利要求中所用的,单数形式“一个”和“该”包括复数形式。因此,例如,提及“装置”包括两种或多种这种装置的组合,提及“基因融合构建体”包括构建体的混合物等。
除非另外说明,在此所用的所有科技术语具有与本发明所属领域的普通技术人员通常所理解的相同的含义。虽然任何与在此描述类似或等价的方法和材料都可被用于实践中来验证本发明,在此描述了合适的材料和方法的特定实施例。
在对本发明的描述和要求保护的内容中,如下的术语将按照下文给出的定义来使用。
因此,用于本发明的目的,术语“草甘膦”应当被认为是包括任何具有除草活性形式的N-膦酰基甲基甘氨酸(包括任何的其盐)和其他会导致在植物中产生草甘膦阴离子的形式。术语“草甘膦类似物”是指草甘膦的任何结构类似物,这种结构类似物能够以一定水平抑制EPSPS,使得草甘膦类似物能够有效地除草。
如在此所用,术语“草甘膦-N-乙酰转移酶活性”或者“GAT活性”是指催化草甘膦的仲胺基团的乙酰化的能力,例如,如附图1中说明的。“草甘膦-N-乙酰转移酶”或“GAT”是一种催化草甘膦、草甘膦类似物和/或草甘膦主要代谢物(即AMPA或肌氨酸)的氨基基团的乙酰化的酶。在本发明的一些优选方案中,GAT能够将乙酰CoA的乙酰基团转移到草甘膦的仲胺和AMPA的伯胺上。此外,某些GAT还能够将丙酰CoA的丙酰基转移给草甘膦,表明GAT还是一种乙酰转移酶。在此描述的示例性GAT在pH 5-9时具有活性,在约pH 6.5-8.0的范围内具有最佳的活性。可以用本领域熟知的各种动力学参数来量化活性,例如k催化、KM和k催化/KM。可以按照下面的实施例7或实施例19所述,确定这些动力学参数。
术语“多核苷酸”、“核苷酸序列”和“核酸”用于指核苷酸(A、C、T、U、G等,或者天然存在的或者人工的核苷酸类似物)的聚合体,例如DNA或RNA,或其表示方式,例如字符串等,这取决于相关的内容。可以从任何特定的核苷酸序列中确定给定的多核苷酸或者互补的多核苷酸。
类似地,“氨基酸序列”是氨基酸的聚合体(蛋白质、多肽等)或者代表氨基酸聚合体的字符串,这取决于内容。术语“蛋白质”、“多肽”和“肽”在此可以互换使用。
当多核苷酸、多肽或者其他成分部分地或者完全地从通常相关的成分(其他蛋白质、核酸、细胞、合成试剂等)中分离出来时,其是“分离的”。当核酸或者多肽为人造的或者工程化的,或者是来源于人造的或者工程化的蛋白质或核酸时,该核酸或者多肽是“重组的”。例如,多核苷酸被插入到载体或者任何其他异源性位置,例如插入到重组生物体的基因组中,使得该多核苷酸不与自然界中发现的通常位于多核苷酸侧翼的苷酸序列结合,这种多核苷酸是重组的多核苷酸。在体外或体内从重组多核苷酸表达得到的蛋白质是重组多肽的一个例子。同样,不出现于自然界中的多核苷酸序列,例如天然存在的基因的变体,是重组的。
术语“草甘膦-N-乙酰转移酶多肽”和“GAT多肽”可以互换使用,用于指在此提供的新多肽家族的任何成员。
术语“草甘膦-N-乙酰转移酶的多核苷酸”和“GAT的多核苷酸”互换使用,用于指编码GAT多肽的多核苷酸。
“亚序列”和“片段”是完整序列的任何部分。
当聚合体的特定单体成分(氨基酸残基、掺入的核苷酸等)的位置对应于所选择的参比多肽或者多核苷酸的相同残基位置时,氨基酸或核苷酸聚合体的编号对应于所选择的氨基酸聚合体或者核酸的编号。
载体是方便用所选择的核酸转导或转化细胞或者方便核酸在细胞中表达的组合物。载体包括,例如质粒、粘粒、病毒、YAC、细菌、多聚赖氨酸、染色体整合载体、附加体载体等。
“多核苷酸或者氨基酸序列的基本全长”是指一条序列的至少约70%、通常至少约80%或者典型地约90%或更长。
如在此所用,“抗体”是指包含一种或多种多肽的蛋白质,该多肽基本上或者部分地由免疫球蛋白基因或者免疫球蛋白基因的片段所编码。已知的免疫球蛋白基因包括κ、λ、α、γ、δ、ε和μ恒定区基因,以及各种各样的免疫球蛋白可变区基因。轻链被分为κ或者λ。重链分为γ、μ、α、δ或ε,它们依次又决定了免疫球蛋白的类型,分别为IgG、IgM、IgA、IgD和IgE。典型的免疫球蛋白(抗体)结构单位包括一种四聚体。每一个四聚体由相同的两对多肽链组成,每对多肽具有一条“轻链”(约25kD)和一条“重链”(约50-70kD)。各条链的N末端定义了由大约100-110个或更多氨基酸组成的主要负责抗体识别的可变区。术语可变轻链(VL)和可变重链(VH)分别指这些轻链和重链。抗体以完整的免疫球蛋白存在或由以各种肽酶消化产生的已经清楚地表征的片段存在。因此,例如,胃蛋白酶在铰链区的二硫键处消化抗体,以产生F(ab)′2,它是Fab的二聚体,Fab本身是一条通过二硫键连接到VH-CH1上的轻链。F(ab)′2可以在温和的条件下被还原,断裂铰链区内的二硫键,从而将(Fab′)2二聚体转化为Fab′单体。Fab′单体实质上是具有部分铰链区的Fab(参见Paul,编辑(1998)Fundamental Immunology(第4版,Raven出版社,纽约州),关于其他抗体片段的更加详细的描述)。虽然按照对完整抗体的消化来定义各种抗体片段,技术人员将会预期,可以用化学方法或者通过采用重组DNA技术来重新合成这种Fab′片段。因此,在此使用的术语抗体还包括修饰整个抗体或者用重组DNA技术重新合成所生产的抗体片段。抗体包括单链抗体,包括单链Fv(sFv)抗体,其中可变重链和可变轻链被连接在一起(直接地或者通过肽接头),形成一条连续的多肽。
“叶绿体转运肽”是一种氨基酸序列,它与一种蛋白质一起被翻译,将该蛋白质导向叶绿体或者存在于制造该蛋白质的细胞中的其他质体类型。“叶绿体转运序列”是指编码叶绿体转运肽的核苷酸序列。
“信号肽”是一种氨基酸序列,其与一种蛋白质一起被翻译,将该蛋白质导向分泌系统中(Cbrispeels(1991)Ann.Rev.PlantPhys.Plant Mol.Biol.42:21-53)。如果要将此蛋白质导向液泡,可以进一步添加液泡定向信号,或者如果导向内质网,则可以添加内质网保留信号。如果蛋白质将被导向细胞核,则任何存在的信号肽应当被去除并插入所包括的细胞核定位信号(Raikhel(1992)PlantPhys.100:1627-1632)。
术语“多样化”和“多样性”应用于多核苷酸时,是指产生大量被修饰形式的亲代的多核苷酸,或者大量亲代的多核苷酸。当多核苷酸编码多肽时,多核苷酸的核苷酸序列的多样性可以导致相应的被编码多肽的多样性,例如编码大量多肽变体的多核苷酸的各种库。在本发明的一些实施方案中,该序列的多样性是通过筛选/选择多样化的多核苷酸的文库中的具有期望的功能性特征的变体而获得的,这些变体例如编码具有增强的功能特征的GAT多肽的多核苷酸。
术语“编码”是指核苷酸序列编码一种或多种氨基酸的能力。该术语不要求起始或终止密码子。氨基酸序列可以在由多核苷酸序列及其补体提供的六个不同阅读框的任何一个中被编码。
当在此所用时,术语“人工变体”是指具有GAT活性的多肽,该多肽由被修饰的GAT多核苷酸编码,例如被修饰形式的SEQ ID NO:516、517、518、519、520、521、522、523、524、525、526、527、528、529、530、531、532、533、534、535、536、537、538、539、540、541、542、543、544、545、546、547、548、549、550、551、552、553、554、555、556、557、558、559、560、561、562、563、564、565、566、567、620、622、624、626、628、630、632、634、636、638、640、642、644、646、648、650、652、654、656、658、660、662、664、666、668、670、672、674、676、678、680、682、684、686、688、690、692、694、696、698、700、702、704、706、708、710、712、714、716、718、720、722、724、726、728、730、732、734、736、738、740、742、744、746、748、750、752、754、756、758、760、762、764、768、770、772、774、776、778、780、782、784、786、788、790、792、794、796、798、800、802、804、806、808、810、812、814、816、818、820、822、824、832、834、836、838、840、842、844、846、848、850、852、854、856、858、860、862、864、866、868、870、872、874、876、878、880、882、884、886、888、890、892、894、896、898、900、902、904、906、908、910、912、914、916、918、920、922、924、926、928、930、932、933、934、935、936、937、938、939、940、941、942、943、944、945、947、949、951和952中的任何一种或者从生物体中分离的天然存在的GAT多核苷酸的被修饰形式所编码。当在合适的宿主中被表达时,从被修饰的多核苷酸中生成人工变体,这些被修饰的多核苷酸是通过修饰GAT多核苷酸的人为干预而获得的。
术语“核酸构建体”或者“多核苷酸构建体”是指核酸分子,单链的或者双链的,从天然存在的基因中分离得到,或者被修饰而以自然界中不存在的方式含有核酸片段。当核酸构建体含有表达本发明的编码序列所需的控制序列时,术语核酸构建体与术语“表达盒”是同义的,
术语“控制序列”在此被定义来包括所有成分,这些成分对于表达本发明的多肽是必需的或者有利的。每种控制序列对于编码多肽的核苷酸序列来说,是天然的或者外来的。每种控制序列包括,但是不限于,前导序列、多聚腺苷酸序列、前肽序列、启动子序列、信号肽序列和转录终止序列。控制序列至少包括启动子以及转录和翻译终止信号。可以给控制序列提供带接头,目的是导入特定的限制性位点,以便将控制序列与编码多肽的核苷酸序列的编码区连接起来。
术语“可操作地连接”在此被定义为一种构象,其中控制序列被适当地放置在与DNA序列的编码序列相关的位置上,使得控制序列能够指导多肽的表达。
当用于此时,术语“编码序列”包括直接具体确定其蛋白质产物的氨基酸序列的核苷酸序列。编码序列的边界通常由以ATG起始密码子开始的开放阅读框确定。编码序列典型地包括DNA、cDNA和/或重组核苷酸序列。
在本文中,术语“表达”包括任何与多肽生产相关的步骤,包括但是不限于转录、转录后修饰、翻译、翻译后修饰和分泌。
在本文中,术语“表达载体”包括线性的或者环状的DNA分子,其包括编码本发明的多肽的区段,并且被可操作地连接到其他用于其转录的区段上。
如在此所用,术语“宿主细胞”包括容易用核酸构建体转化的任何细胞类型。
术语“植物”包括整株植物、枝条营养器官/结构(例如叶、茎和块茎)、根、花和花器官/结构(例如苞片、萼片、花瓣、雄蕊、心皮、花药和胚珠)、种子(包括胚胎、胚乳和种皮)和果实(成熟的卵巢)、植物组织(例如微管组织、地面组织等)和细胞(例如保卫细胞、卵细胞、毛状体等),以及它们的后代。用于本发明的方法中的植物类型通常包括适合于转化技术的高等植物和低等植物的种类范围,包括被子植物(单子叶植物和双子叶植物)、裸子植物、蕨类植物和多细胞藻类。它包括各种倍数水平的植物,包括非整倍体、多倍体、二倍体、单倍体和半合子。
术语“异源的”用于此来描述两种或者多种元件之间的关系,表明这些元件之间实质上是不相似的。因此,例如,如果多核苷酸来源于外来物种,或者如果来自同一物种但是对其原始形式进行了修饰,则多核苷酸序列与生物体或者第二条多核苷酸序列是“异源的”。例如,启动子被可操作地连接到异源的编码序列上,是指编码序列来自不同于产生该启动子的物种的物种,或者如果来源于相同的物种,则编码序列在自然情况下与此启动子无关(例如,遗传工程化的编码序列或者来自不同生态型或者变体的等位基因)。异源多肽的一个例子是在转基因生物体中由重组多核苷酸表达得到的多肽。异源的多核苷酸或者多肽都是重组分子的形式。
在此还定义或者另外地表征了各种其他术语。
草甘膦-N-乙酰转移酶
在一个方面中,本发明提供了一个在此被称作为“草甘膦-N-乙酰转移酶”、“GAT”或者“GAT酶”的分离的或者重组的酶的新家族。GAT是具有GAT活性的酶,优选地是具有足以赋予工程化来表达GAT的转基因植物具有某种程度的草甘膦耐受性的活性。GAT的某些例子包括GAT多肽,将在下文被更加详细地描述。
GAT介导的草甘膦耐受性是GAT活性、转基因植物中的GAT表达水平、特定植物和许多包括但是不限于除草剂应用的性质和时间安排等的其他因素的复合函数。本领域技术人员无需过多的试验就可以确定在特定条件下产生草甘膦耐受性所需的GAT活性水平。
可以用常规的动力学参数k催化、KM和k催化/KM来表征GAT活性。k
催化被认为是乙酰化速度的量度,特别是在高底物浓度时,KM是GAT对其底物(例如乙酰CoA、丙酰CoA和草甘膦)的亲合性的量度,而k催
化/KM是同时考虑底物亲合性和催化速度时的催化效率的量度。在底物浓度至少部分地是速度的限制因素时,k催化/KM是尤其重要的。总之,与具有较低的k催化或者k催化/KM的另一种GAT相比,具有较高的k催化或者k催化/KM的GAT是更有效的催化剂。与具有较高的KM的另一种GAT相比,具有较低的KM的GAT是更加有效的催化剂。因此,为了确定一种GAT是否比另一种GAT更加有效,可以比较两种酶的动力学参数。k催化、k催化/KM和KM的相对重要性是变化的,取决于期望GAT起作用的条件,例如期望的草甘膦的有效浓度与草甘膦的KM相关。还可以根据大量的功能性特征的任何一种来表征GAT活性,这些功能特征包括但是不限于稳定性、对抑制的易感性或者被其他分于活化。
草甘膦-N-乙酰转移酶多肽
在一个方面中,本发明提供一个在此被称作为“草甘膦-N-乙酰转移酶多肽”或者“GAT多肽”的分离的或者重组多肽的新家族。GAT多肽的特征是它们与新的GAT家族的结构相似性。许多但并不是所有的GAT多肽是GAT。区别在于GAT是以功能定义的,而GAT多肽是根据结构定义的。GAT多肽的一个亚组由那些具有GAT活性的GAT多肽组成,GAT多肽优选能够起使有效地表达该蛋白的转基因植物具有草甘膦抗性的作用。一些用于赋予对草甘膦的耐受性的优选GAT多肽具有至少1min-1,或者更加优选至少10min-1、100min-1或者1000min-1的k催化。其他优选用于赋予草甘膦耐受性的优选GAT多肽具有不超过100mM,或更加优选不超过10mM、1mM或者0.1mM的KM。还有其他用于赋予草甘膦耐受性的优选GAT多肽具有至少10mM-1min-1、100mM-1min-1、1000mM-1min-1或者10,000mM-1min-1的k催化/KM。
示例性的GAT多肽已经从各种细菌菌株中分离和表征。已经被分离和表征的单体GAT多肽的一个例子具有约17kD的分子半径。一种分离自地衣芽孢杆菌菌株的示例性GAT酶,SEQ ID NO:7,具有约2.9mM的对草甘膦的Km,和约2μM的对乙酰CoA的Km,并且k催化等于6/分钟。
术语“GAT多肽”是指任何包含氨基酸序列的多肽,采用B1OSUM62矩阵方法,将氨基酸序列与选自SEQ ID NO:300、445或457的氨基酸序列最佳地比对,产生至少为460的相似性分值,存在一个缺口罚11分,延伸一个缺口罚1分,其中至少如下位置之一遵从如下的限制:(i)在位置18和38上,为Z5氨基酸残基;(ii)在位置62上,为Z1氨基酸残基;(iii)在位置124上,为Z6氨基酸残基;和(iv)在位置144上,为Z2氨基酸残基,其中:Z1是选自由A、I、L、M和V组成的组的氨基酸残基;Z2是选自由F、W和Y组成的组的氨基酸残基;Z5是选自由D和E组成的组的氨基酸残基;和Z6是选自由C、G和P组成的组的氨基酸残基。本发明的一些方面涉及包含氨基酸序列的GAT多肽,采用B1OSUM62矩阵方法,,存在一个缺口罚11分,延伸一个缺口罚1分,将该氨基酸序列与选自由SEQ ID NO:300、445或457组成的组的氨基酸序列最佳地比对,产生至少440、445、450、455、460、465、470、475、480、485、490、495、500、505、510、515、520、525、530、535、540、545、550、555、560、565、570、575、580、585、590、595、600、605、610、615、620、625、630、635、640、645、650、655、660、665、670、675、680、685、690、695、700、705、710、715、720、725、730、735、740、745、750、755或760的相似性分值,其中至少如下位置之一遵从如下的限制:(i)在位置18和38上,为Z5氨基酸残基;(ii)在位置62上,为Z1氨基酸残基;(iii)在位置124上,为Z6氨基酸残基;和(iv)在位置144上,为Z2氨基酸残基,其中:Z1是选自由A、I、L、M和V组成的组的氨基酸残基;Z2是选自由F、W和Y组成的组的氨基酸残基;Z5是选自由D和E组成的组的氨基酸残基;和Z6是选自由C、G和P组成的组的氨基酸残基。
两条序列被“最佳地比对”,是指当它们被比对来进行相似性评分时,采用特定的氨基酸取代矩阵(例如B1OSUM62)、缺口存在罚分和缺口延伸罚分,以获得该对序列的可能最高得分。氨基酸取代矩阵及其在量化两条序列之间的相似性中的用途是本领域熟知的,并且被描述在例如Dayhoff等人(1978)“A model of evolutionary changein proteins″in″Atlas of Protein Sequence and Structure,”第5卷,增刊3(M.O.Dayhoff编辑),第345-352页Natl.Biomed.Res.Found.,华盛顿地区以及Henikoff等人(1992)Proc.Nat′LAcad.Sci.USA 89:10915-10919中。B1OSUM62矩阵(附图10)在缺口B1AST 2.0等序列比对方案中通常被用作默认的评分取代矩阵。对在被比对的序列之一中导入单个氨基酸缺口进行缺口存在罚分,而对于在已经开口的缺口中插入的各个额外的空氨基酸位置,进行缺口延伸罚分。比对是由各条序列开始和终止比对的氨基酸位置来确定的,任选地在一条或两条序列中插入一个或多个缺口来确定,以获得最高的可能分值。尽管最佳比对和评分可以手工完成,但是使用计算机辅助的序列比对算法方便该过程,这种算法如缺口B1AST 2.0,在Altschul等人(1997)Nucl.Acids Res.25:3389-3402中被描述,公众可以从National Center for Biotechnology Information(NCBI)网站(www.ncbi.nlm.nih.gov)上获得。最佳比对,包括多重比对,可用如PSI-B1AST来进行,PSI-B1AST可从NCBI网站获得,其描述见Altschul等人(1997)Nucl.Acids Res.25:3389-3402。
至于与参比序列最佳地比对的氨基酸序列,氨基酸残基与在比对中相配对的参比序列的位置“相对应”。用根据相对于N末端的位置,随后确定参比序列中的各个氨基酸的编号来表示“位置”。例如,在SEQ ID NO:300中,位置1为M,位置2为I,位置3为E等。当测试序列最佳地与SEQ ID NO:300比对时,测试序列中与位置3的E比对的残基被认为与SEQ ID NO:300的“位置3相对应”。当确定最佳的序列比对时,必需考虑缺失、插入、平截、融合等,总之,通过简单地从N末端开始计数来确定测试序列中的氨基酸残基的编号,不必与其在参比序列中的相应位置的编号相同。例如,在被比对的测试序列中存在缺失的情形下,不存在对应于参比序列的缺失位点的位置上的氨基酸。当在被比对的参比序列中存在一个插入时,该插入将不与参比序列中的任何氨基酸位置相对应。在为平截或者融合的情况下,则在参比或者被比对的序列中,存在与相应序列中的任何氨基酸不对应的氨基酸区段。
术语“GAT多肽”还指包含氨基酸序列的任何多肽,该氨基酸序列选自如下组成的组:(a)与SEQ ID NO:577至少98%相同的氨基酸序列;(b)与SEQ ID NO:578至少97%相同的氨基酸序列;(c)与SEQ ID NO:621至少97%相同的氨基酸序列;(d)与SEQ ID NO:579至少98%相同的氨基酸序列;(e)与SEQ ID NO:602至少98%相同的氨基酸序列;(f)与SEQ ID NO:697至少95%相同的氨基酸序列;(g)与SEQ ID NO:712至少96%相同的氨基酸序列;(h)与SEQ ID NO:613至少97%相同的氨基酸序列;(i)与SEQ ID NO:677至少89%相同的氨基酸序列;(j)与SEQ ID NO:584至少95%相同的氨基酸序列;(k)与SEQ ID NO:707至少98%相同的氨基酸序列;(l)与SEQ ID NO:616至少98%相同的氨基酸序列;(m)与SEQ IDNO:612至少96%相同的氨基酸序列;和(n)与SEQ ID NO:590至少98%相同的氨基酸序列。
术语“GAT多肽”还指任何多肽,该多肽包含与SEQ ID NO:677的氨基酸序列的残基1-96具有至少89%序列同一性的氨基酸序列;与SEQ ID NO:697的氨基酸序列的残基1-96具有至少95%序列同一性的氨基酸序列;与选自SEQ ID NO:584、612和721的氨基酸序列的残基1-96具有至少96%序列同一性的氨基酸序列;与选自SEQ ID NO:578、613和621的氨基酸序列的残基1-96具有至少97%序列同一性的氨基酸序列;与选自SEQ ID NO:577、579、590、602、616和707的氨基酸序列的残基1-96具有至少98%序列同一性的氨基酸序列。
术语“GAT多肽”还指任何多肽,该多肽包含与SEQ ID NO:677的氨基酸序列的残基51-146具有至少89%序列同一性的氨基酸序列;与SEQ ID NO:697的氨基酸序列的残基51-146具有至少95%序列同一性的氨基酸序列;与选自SEQ ID NO:584、612和721的氨基酸序列的残基51-146具有至少96%序列同一性的氨基酸序列;与选自SEQID NO:578、613和621的氨基酸序列的残基51-146具有至少97%序列同一性的氨基酸序列;与选自SEQ ID NO:577、579、590、602、616和707的氨基酸序列的残基51-146具有至少98%序列同一性的氨基酸序列。
术语“GAT多肽”还指任何多肽,该多肽包含选自由如下组成的组的氨基酸序列:(a)与SEQ ID NO:919的位置2-146至少96%相同的氨基酸序列;(b)与SEQ ID NO:929的位置2-146至少97%相同的氨基酸序列;(c)与SEQ ID NO:847的位置2-146至少98%相同的氨基酸序列;(d)与SEQ ID NO:851位置2-146至少98%相同的氨基酸序列;(e)与SEQ ID NO:853的位置2-146至少98%相同的氨基酸序列;(f)与SEQ ID NO:855的位置2-146至少98%相同的氨基酸序列(例如SEQ ID NO:835或855);(g)与SEQ ID NO:857的位置2-146至少98%相同的氨基酸序列;(h)与SEQ ID NO:861的位置2-146至少98%相同的氨基酸序列(例如,SEQ ID NO:839、861或者883);(i)与SEQ ID NO:871的位置2-146至少98%相同的氨基酸序列;(j)与SEQ ID NO:875的位置2-146至少98%相同的氨基酸序列;(k)与SEQ ID NO:881的位置2-146至少98%相同的氨基酸序列;(l)与SEQ ID NO:885的位置2-146至少98%相同的氨基酸序列;(m)与SEQ ID NO:887的位置2-146至少98%相同的氨基酸序列;(n)与SEQ ID NO:889的位置2-146至少98%相同的氨基酸序列;(o)与SEQ ID NO:893的位置2-146至少98%相同的氨基酸序列;(p)与SEQ ID NO:897的位置2-146至少98%相同的氨基酸序列;(q)与SEQ ID NO:899的位置2-146至少98%相同的氨基酸序列;(r)与SEQ ID NO:909的位置2-146至少98%相同的氨基酸序列;(s)与SEQ ID NO:911的位置2-146至少98%相同的氨基酸序列;(t)与SEQ ID NO:837的位置2-146至少99%相同的氨基酸序列;(u)与SEQ ID NO:841的位置2-146至少99%相同的氨基酸序列;(v)与SEQ ID NO:865的位置2-146至少99%相同的氨基酸序列;(w)与SEQ ID NO:869的位置2-146至少99%相同的氨基酸序列;和(x)与SEQ ID NO:879的位置2-146至少99%相同的氨基酸序列。
术语“GAT多肽”还指任何多肽,该多肽包含与SEQ ID NO:929的位置2-146至少95%相同的氨基酸序列,并且在对应于SEQ ID NO:929的位置33的氨基酸位置上包含Gly和Asn残基。
术语“GAT多肽”还指任何多肽,该多肽包含与在此公开的示例性GAT多肽共有至少60%、65%、70%、75%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%、99%或者更高的序列同一性的氨基酸序列。因此,例如本发明的GAT多肽包含氨基酸序列,该氨基酸序列与SEQ ID NO:953、954、955、956、957、958、959、960、961、962、963、964、965、966、967、968、969、970、971和972的任何一种共有至少60%、65%、70%、75%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%、99%或者更高的序列同一性。
如在此所用,术语“同一性”或者“同一性百分数”用于具体的一对被比对的氨基酸序列时,是指通过ClustalW分析(版本W 1.8,可以从European Bioinformatics Institute,剑桥,英国获得)获得的氨基酸序列同一性百分数,计算比对中相同配对的数目,并用该相同配对的数目除以(i)被比对序列的长度和(ii)96中较大的一个,使用如下的默认ClustalW参数来进行缓慢/精确切换配对比对——缺口罚分:10;延伸罚分:0.10;蛋白质权重矩阵:Goimet系列;DNA权重矩阵:IUB;扳手缓慢/快速配对方式比对=缓慢或者完全比对。
另一个方面,本发明提供一种分离的或者重组的多肽,其包含选自由如下构成的组的氨基酸序列中的至少20个,或者可替代地,至少50个、至少75个、至少100个、至少125个、至少130个、至少135个、至少140个、至少141个、至少142个、至少143个、至少144个或者至少145个连续的氨基酸:(a)与SEQ ID NO:577至少98%相同的氨基酸序列;(b)与SEQ ID NO:578至少97%相同的氨基酸序列;(c)与SEQ ID NO:621至少97%相同的氨基酸序列;(d)与SEQ IDNO:579至少98%相同的氨基酸序列;(e)与SEQ ID NO:602至少98%相同的氨基酸序列;(f)与SEQ ID NO:697至少95%相同的氨基酸序列;(g)与SEQ ID NO:712至少96%相同的氨基酸序列;(h)与SEQ ID NO:613至少97%相同的氨基酸序列;(i)与SEQ ID NO:677至少89%相同的氨基酸序列;(j)与SEQ ID NO:584至少95%相同的氨基酸序列;(k)与SEQ ID NO:707至少98%相同的氨基酸序列;(l)与SEQ ID NO:616至少98%相同的氨基酸序列;(m)与SEQ ID NO:612至少96%相同的氨基酸序列;和(n)与SEQ ID NO:590至少98%相同的氨基酸序列。
另一个方面,本发明提供包含选自由如下组成的组的氨基酸序列的残基2-146的多肽:SEQ ID NO:568、569、570、571、572、573、574、575、576、577、578、579、580、581、582、583、584、585、586、587、588、589、590、591、592、593、594、595、596、597、598、599、600、601、602、603、604、605、606、607、608、609、610、611、612、613、614、615、616、617、618、619、621、623、625、627、629、631、633、635、637、639、641、643、645、647、649、651、653、655、657、659、661、663、665、667、669、671、673、675、677、679、681、683、685、687、689、691、693、695、697、699、701、703、705、707、709、711、713、715、717、719、721、723、725、727、729、731、733、735、737、739、741、743、745、747、749、751、753、755、757、759、761、763、765、767、769、771、773、775、777、779、781、783、785、787、789、791、793、795、797、799、801、803、805、807、809、811、813、815、817、819、821、823和825。在本发明的一些实施方案中,多肽的氨基酸序列在对应于参比的氨基酸序列的位置2的氨基酸的N末端一侧上包含Met、Met-Ala或者Met-Ala-Ala。
一些优选的本发明的GAT多肽可以与选自由SEQ ID NO:300、445或457组成的组的参比氨基酸序列最佳地比对,采用B1OSUM62矩阵方法,存在一个缺口罚11分,延伸一个缺口罚1分,生成至少为460的相似性分值,其中至少如下位置之一遵从如下的限制:(i)在位置18和38上,为Z5氨基酸残基;(ii)在位置62上,为Z1氨基酸残基;(iii)在位置124上,为Z6氨基酸残基;和(iv)在位置144上,为Z2氨基酸残基,其中:Z1是选自由A、I、L、M和V组成的组的氨基酸残基;Z2是选自由F、W和Y组成的组的氨基酸残基;Z5是选自由D和E组成的组的氨基酸残基;和Z6是选自由C、G和P组成的组的氨基酸残基,并且进一步地,氨基酸序列中对应于如下位置的氨基酸残基至少90%地遵从如下限制:(a)在位置2、4、15、19、26、28、31、45、51、54、86、90、91、97、103、105、106、114、123、129、139和/或145上,氨基酸残基为B1;和(b)在位置3、5、8、10、11、14、17、24、27、32、37、47、48、49、52、57、58、61、63、68、69、79、80、82、83、89、92、100、101、104、119、120、125、126、128、131和/或143上,氨基酸残基为B2;其中B1为选自由A、I、L、M、F、W、Y和V组成的组的氨基酸;而B2是选自由R、N、D、C、Q、E、G、H、K、P、S和T组成的组的氨基酸。当用于表示氨基酸或者氨基酸残基时,单字母名称A、C、D、E、F、G、H、I、K、L、M、N、P、Q、R、S、T、V、W和Y具有本领域所用的标准含义,并且提供在表1中。
一些优选的本发明的GAT多肽可以与选自由SEQ ID NO:300、445或457组成的组的参比氨基酸序列最佳地比对,采用B1OSUM62矩阵方法,存在一个缺口罚11分,延伸一个缺口罚1分,生成至少460的相似性分值,其中至少如下位置之一遵从如下的限制:(i)在位置18和38上,为Z5氨基酸残基;(ii)在位置62上,为Z1氨基酸残基;(iii)在位置124上,为Z6氨基酸残基;和(iv)在位置144上,为Z2氨基酸残基,其中:Z1是选自由A、I、L、M和V组成的组的氨基酸残基;Z2是选自由F、W和Y组成的组的氨基酸残基;Z5是选自由D和E组成的组的氨基酸残基;和Z6是选自由C、G和P组成的组的氨基酸残基,并且进一步地,氨基酸序列中对应于如下位置的氨基酸残基至少80%地遵从如下限制:(a)在位置2、4、15、19、26、28、31、45、51、54、86、90、91、97、103、105、106、114、123、129、139和/或145上,氨基酸残基为Z1;和(b)在位置31和/或45上,氨基酸残基为Z2;(c)在位置8上,氨基酸残基为Z3;(d)在位置89上,氨基酸残基为Z3或者Z6;(e)在位置82,92,101和/或120上,氨基酸残基为Z4;(f)在位置3、11、27和/或79上,氨基酸残基为Z5;(g)在位置18上,氨基酸残基为Z4或Z5;(h)在位置123上,氨基酸残基为Z1或Z2;(i)在位置12,33、35、39、53、59、112、132、135、140和/或146上,氨基酸残基为Z1或Z3;(j)在位置30上,氨基酸残基为Z1;(k)在位置6上,氨基酸残基为Z6;(l)在位置81上,氨基酸残基为Z2或Z4;(m)在位置113上,氨基酸残基为Z3;(n)在位置138上,氨基酸残基为Z4;(o)在位置142上,氨基酸残基为Z2;(p)在位置57和/或126上,氨基酸残基为Z3或Z4;(q)在位置5,17和61上,氨基酸残基为Z4;(r)在位置24上,氨基酸残基为Z3;(s)在位置104上,氨基酸残基为Z5;(t)在位置52和/或69上,氨基酸残基为Z3;(u)在位置14和/或119上,氨基酸残基为Z5;(v)在位置10、32、63和/或83上,氨基酸残基为Z5;(w)在位置48和/或80上,氨基酸残基为Z6;(x)在位置40上,氨基酸残基为Z1或Z2;(y)在位置96上,氨基酸残基为Z3或Z5;(z)在位置65上,氨基酸残基为Z3、Z4或Z6;(aa)在位置84和/或115上,氨基酸残基为Z3;(ab)在位置93上,氨基酸残基为Z4;(ac)在位置130上,氨基酸残基为Z2;(ad)在位置58上,氨基酸残基为Z3、Z4或者Z6;(ae)在位置47上,氨基酸残基为Z4或Z6;(af)在位置49和/或100上,氨基酸残基为Z3或Z4;(ag)在位置68上,氨基酸残基为Z4或Z5;(ah)在位置143上,氨基酸残基为Z4;(ai)在位置131上,氨基酸残基为Z5;(aj)在位置125和/或128上,氨基酸残基为Z5;(ak)在位置67上,氨基酸残基为Z3或Z4;(al)在位置60上,氨基酸残基为Z5;和(am)在位置37上,氨基酸残基为Z4或Z6;其中Z1是选自由A、I、L、M和V组成的组的氨基酸残基;Z2是选自由F、W和Y组成的组的氨基酸残基;Z3是选自由N、Q、S和T组成的组的氨基酸;Z4是选自由R、H和K组成的组的氨基酸;Z5是选自由D和E组成的组的氨基酸残基;和Z6是选自由C、G和P组成的组的氨基酸残基。
一些优选的本发明的GAT多肽在对应于在(a)-(am)中指定的位置的氨基酸序列中还包含氨基酸残基,其中至少90%地遵从在(a)-(am)中指定的氨基酸残基的限制。
一些优选的本发明的GAT多肽在对应于如下位置的氨基酸序列中还包含氨基酸残基,其中至少90%地遵从如下限制:(a)在位置1、7、9、13、20、36、42、46、50、56、64、70、72、75、76、78、94、98、107、110、117、118、121和/或141上,氨基酸残基为B1;和(b)在位置16、21、22、23、25、29、34、41、43、44、55、66、71、73、74、77、85、87、88、95、99、102、108、109、111、116、122、127、133、134、136和/或137上,氨基酸残基为B2;其中B1为选自由A、I、L、M、F、W、Y和V组成的组的氨基酸;而B2是选自由R、N、D、C、Q、E、G、H、K、P、S和T组成的组的氨基酸。
一些优选的本发明的GAT多肽在对应于如下位置的氨基酸序列中还包含氨基酸残基,其中至少90%地遵从如下限制:(a)在位置1、7、9、13、20、42、46、50、56、64、70、72、75、76、78、94、98、107、110、117、118、121和/或141上,氨基酸残基为B1;和(b)在位置16、21、22、23、25、29、34、36、41、43、44、55、66、71、73、74、77、85、87、88、95、99、102、108、109、111、116、122、127、133、134、136和/或137上,氨基酸残基为B2;其中B1为选自由A、I、L、M、F、W、Y和V组成的组的氨基酸;而B2是选自由R、N、D、C、Q、E、G、H、K、P、S和T组成的组的氨基酸。
一些优选的本发明的GAT多肽在对应于如下位置的氨基酸序列中还包含氨基酸残基,其中至少90%地遵从如下限制:(a)在位置1、7、9、13、20、42、50、72、75、76、78、94、98、110、121和/或141上,氨基酸残基为Z1;(b)在位置13、46、56、70、107、117和/或118上,氨基酸残基为Z2;(c)在位置23、55、71、77、88和/或109上,氨基酸残基为Z3;(d)在位置16、21、41、73、85、99和/或111上,氨基酸残基为Z4;(e)在位置34和/或95上,氨基酸残基为Z5;(f)在位置22、25、29、43、44、66、74、87、102、108、116、122、127、133、134、136和/或137上,氨基酸残基为Z6;其中Z1是选自由A、I、L、M和V组成的组的氨基酸残基;Z2是选自由F、W和Y组成的组的氨基酸残基;Z3是选自由N、Q、S和T组成的组的氨基酸;Z4是选自由R、H和K组成的组的氨基酸;Z5是选自由D和E组成的组的氨基酸残基;和Z6是选自由C、G和P组成的组的氨基酸残基。
一些优选的本发明的GAT多肽在位置36上还包含选自由Z1和Z3组成的组的氨基酸残基。一些本发明的优选GAT多肽在位置64上还包含选自由Z1和Z2组成的组的氨基酸残基。
一些优选的本发明的GAT多肽在对应于如下位置的氨基酸序列中还包含氨基酸残基,其中至少80%地遵从如下限制:(a)在位置2上,氨基酸残基为I或L;(b)在位置3上,氨基酸残基为E;(c)在位置4上,氨基酸残基为V或I;(d)在位置5上,氨基酸残基为K;(e)在位置6上,氨基酸残基为P;(f)在位置8上,氨基酸残基为N;(g)在位置10上,氨基酸残基为E;(h)在位置11上,氨基酸残基为D或E;(i)在位置12上,氨基酸残基为T;(j)在位置14上,氨基酸残基为E或D;(k)在位置15上,氨基酸残基为L;(l)在位置17上,氨基酸残基为H;(m)在位置18上,氨基酸残基为R、E或K;(n)在位置19上,氨基酸残基为I或V;(o)在位置24上,氨基酸残基为Q;(p)在位置26上,氨基酸残基为M、L、V或I;(q)在位置27上,氨基酸残基为E;(r)在位置28上,氨基酸残基为A或V;(s)在位置30上,氨基酸残基为M;(t)在位置31上,氨基酸残基为Y或F;(u)在位置32上,氨基酸残基为E或D;(v)在位置33上,氨基酸残基为T或S;(w)在位置35上,氨基酸残基为L;(x)在位置37上,氨基酸残基为R、G、E或Q;(y)在位置39上,氨基酸残基为A或S;(z)在位置40上,氨基酸残基为F或L;(aa)在位置45上,氨基酸残基为Y或F;(ab)在位置47上,氨基酸残基为R或G;(ac)在位置48上,氨基酸残基为G;(ad)在位置49上,氨基酸残基为K、R或Q;(ae)在位置51上,氨基酸残基为I或V;(af)在位置52上,氨基酸残基为S;(ag)在位置53上,氨基酸残基为I或V;(ah)在位置54上,氨基酸残基为A;(ai)在位置57上,氨基酸残基为H或N;(aj)在位置58上,氨基酸残基为Q、K、R或P;(ak)在位置59上,氨基酸残基为A;(al)在位置60上,氨基酸残基为E;(am)在位置61上,氨基酸残基为H或R;(an)在位置63上,氨基酸残基为E或D;(ao)在位置65上,氨基酸残基为E、P或Q;(ap)在位置67上,氨基酸残基为Q或R;(aq)在位置68上,氨基酸残基为K或E;(ar)在位置69上,氨基酸残基为Q;(as)在位置79上,氨基酸残基为E;(at)在位置80上,氨基酸残基为G;(au)在位置81上,氨基酸残基为Y、H或F;(av)在位置82上,氨基酸残基为R;(aw)在位置83上,氨基酸残基为E或D;(ax)在位置84上,氨基酸残基为Q;(ay)在位置86上,氨基酸残基为A;(az)在位置89上,氨基酸残基为G、T或S;(ba)在位置90上,氨基酸残基为L;(bb)在位置91上,氨基酸残基为L、I或V;(bc)在位置92上,氨基酸残基为R或K;(bd)在位置93上,氨基酸残基为H;(be)在位置96上,氨基酸残基为E或Q;(bf)在位置97上,氨基酸残基为I;(bg)在位置100上,氨基酸残基为K或N;(bh)在位置101上,氨基酸残基为K或R;(bi)在位置103上,氨基酸残基为A或V;(bj)在位置104上,氨基酸残基为D;(bk)在位置105上,氨基酸残基为M、L或I;(Bl)在位置106上,氨基酸残基为L;(bm)在位置112上,氨基酸残基为T或A;(bn)在位置113上,氨基酸残基为S或T;(bo)在位置114上,氨基酸残基为A;(bp)在位置115上,氨基酸残基为S;(bq)在位置119上,氨基酸残基为K或R;(br)在位置120上,氨基酸残基为K或R;(bs)在位置123上,氨基酸残基为F或L;(bt)在位置125上,氨基酸残基为E;(bu)在位置126上,氨基酸残基为Q或H;(bv)在位置128上,氨基酸残基为E或D;(bw)在位置129上,氨基酸残基为V或I;(bx)在位置130上,氨基酸残基为F;(by)在位置131上,氨基酸残基为D或E;(bx)在位置132上,氨基酸残基为T;(ca)在位置135上,氨基酸残基为V;(cb)在位置138上,氨基酸残基为H;(cc)在位置139上,氨基酸残基为I;(cd)在位置140上,氨基酸残基为L或M;(ce)在位置142上,氨基酸残基为Y;(cf)在位置143上,氨基酸残基为K或R;(cg)在位置145上,氨基酸残基为L或I;和(ch)在位置146上,氨基酸残基为T。
一些优选的本发明的GAT多肽在对应于上面的(a)-(ch)中指定的位置的氨基酸序列中还包含氨基酸残基,其中至少90%地遵从在(a)-(ch)中指定的氨基酸残基的限制。
一些优选的本发明的GAT多肽可以与选自由SEQ ID NO:300、445或457组成的组的参比氨基酸序列最佳地比对,采用B1OSUM62矩阵方法,存在一个缺口罚11分,延伸一个缺口罚1分,产生至少为460的相似性分值,其中至少如下位置之一遵从如下的限制:(i)在位置18和38上,存在一个Z5氨基酸残基;(ii)在位置62上,存在一个Z1氨基酸残基;(iii)在位置124上,存在一个Z6氨基酸残基;和(iv)在位置144上,存在一个Z2氨基酸残基;其中:Z1是选自由A、I、L、M和V组成的组的氨基酸残基;Z2是选自由F、W和Y组成的组的氨基酸残基;Z5是选自由D和E组成的组的氨基酸残基;和Z6是选自由C、G和P组成的组的氨基酸残基,并且另外地,氨基酸序列对应于如下位置的氨基酸残基至少80%地遵从如下限制:(a)在位置9、76、94和110上,氨基酸残基为A;(b)在位置29和108上,氨基酸残基为C;(c)在位置34上,氨基酸残基为D;(d)在位置95上,氨基酸残基为E;(e)在位置56上,氨基酸残基为F;(f)在位置43、44、66、74、87、102、116、122、127和136上,氨基酸残基为G;(g)在位置41上,氨基酸残基为H;(h)在位置7上,氨基酸残基为I;(i)在位置85上,氨基酸残基为K;(j)在位置20、42、50、78和121上,氨基酸残基为L;(k)在位置1和141上,氨基酸残基为M;(l)在位置23和109上,氨基酸残基为N;(m)在位置22、25、133、134和137上,氨基酸残基为P;(n)在位置71上,氨基酸残基为Q;(o)在位置16、21、73、99和111上,氨基酸残基为R;(p)在位置55上,氨基酸残基为S;(q)在位置77上,氨基酸残基为T;(r)在位置107上,氨基酸残基为W;和(s)在位置13、46、70和118上,氨基酸残基为Y。
一些优选的本发明的GAT多肽还包含氨基酸序列,其中氨基酸残基满足至少如下限制之一:(a)在位置36上,氨基酸残基为M、L、或T;(b)在位置72上,氨基酸残基为L或I;(c)在位置75上,氨基酸残基为M或V;(d)在位置64上,氨基酸残基为L、I或F;(e)在位置88上,氨基酸残基为T或S;和(f)在位置117上,氨基酸残基为Y或F。
一些优选的本发明的GAT多肽还包含氨基酸序列,其中氨基酸残基满足至少如下限制之一:(a)在位置14上,氨基酸残基为D;(b)在位置18上,氨基酸残基为E;(c)在位置26上,氨基酸残基为M或V;(e)在位置30上,氨基酸残基为I;(f)在位置32上,氨基酸残基为D;(g)在位置36上,氨基酸残基为M或T;(i)在位置37上,氨基酸残基为C;(j)在位置38上,氨基酸残基为D;(j)在位置53上,氨基酸残基为V;(k)在位置58上,氨基酸残基为R;(l)在位置61上,氨基酸残基为R;(m)在位置62上,氨基酸残基为L;(n)在位置64上,氨基酸残基为I或F;(o)在位置65上,氨基酸残基为P;(p)在位置72上,氨基酸残基为I;(q)在位置75上,氨基酸残基为V;(r)在位置88上,氨基酸残基为T;(s)在位置89上,氨基酸残基为G;(t)在位置91上,氨基酸残基为L;(u)在位置98上,氨基酸残基为I;(v)在位置105上,氨基酸残基为I;(w)在位置112上,氨基酸残基为A;(x)在位置124上,氨基酸残基为G或C;(y)在位置128上,氨基酸残基为D;(z)在位置140上,氨基酸残基为M;(aa)在位置143上,氨基酸残基为R;和(ab)在位置144上,氨基酸残基为W。
一些优选的本发明的GAT多肽还包含氨基酸序列,其中对应于上面的(a)-(ab)中指定的位置的氨基酸残基至少80%地遵从在(a)-(ab)中指定的氨基酸残基的限制。
一些优选的本发明的GAT多肽具有包含氨基酸残基的氨基酸序列,至少该氨基酸残基之一满足如下的额外限制:(a)在位置41上,氨基酸残基为H;(b)在位置138上,氨基酸残基为H;(c)在位置34上,氨基酸残基为N;和(d)在位置55上,氨基酸残基为S。
一些优选的本发明的GAT多肽包含选自由如下组成的组的氨基酸序列:(a)与SEQ ID NO:577至少98%相同的氨基酸序列;(b)与SEQ ID NO:578至少97%相同的氨基酸序列;(c)与SEQ ID NO:621至少97%相同的氨基酸序列;(d)与SEQ ID NO:579至少98%相同的氨基酸序列;(e)与SEQ ID NO:602至少98%相同的氨基酸序列;(f)与SEQ ID NO:697至少95%相同的氨基酸序列;(g)与SEQ ID NO:712至少96%相同的氨基酸序列;(h)与SEQ ID NO:613至少97%相同的氨基酸序列;(i)与SEQ ID NO:677至少89%相同的氨基酸序列;(j)与SEQ ID NO:584至少95%相同的氨基酸序列;(k)与SEQ ID NO:707至少98%相同的氨基酸序列;(l)与SEQ ID NO:616至少98%相同的氨基酸序列;(m)与SEQ ID NO:612至少96%相同的氨基酸序列;和(n)与SEQ ID NO:590至少98%相同的氨基酸序列。
一些优选的本发明的GAT多肽包含选自由如下组成的组的氨基酸序列:(a)与SEQ ID NO:577至少98%相同的氨基酸序列;(b)与SEQ ID NO:578至少97%相同的氨基酸序列;(c)与SEQ ID NO:621至少97%相同的氨基酸序列;(d)与SEQ ID NO:579至少98%相同的氨基酸序列;(e)与SEQ ID NO:602至少98%相同的氨基酸序列;(f)与SEQ ID NO:697至少95%相同的氨基酸序列;(g)与SEQ ID NO:712至少96%相同的氨基酸序列;(h)与SEQ ID NO:613至少97%相同的氨基酸序列;(i)与SEQ ID NO:677至少89%相同的氨基酸序列;(j)与SEQ ID NO:584至少95%相同的氨基酸序列;(k)与SEQ ID NO:707至少98%相同的氨基酸序列;(l)与SEQ ID NO:616至少98%相同的氨基酸序列;(m)与SEQ ID NO:612至少96%相同的氨基酸序列;和(n)与SEQ ID NO:590至少98%相同的氨基酸序列,其中至少如下位置之一还遵从如下限制:(i)在位置18和38上,存在一个Z5氨基酸残基;(ii)在位置62,存在一个Z1氨基酸残基;(iii)在位置124上,存在一个Z6氨基酸残基;和(iv)在位置144上,存在一个Z2氨基酸残基,其中:其中Z1是选自由A、I、L、M和V组成的组的氨基酸残基;Z2是选自由F、W和Y组成的组的氨基酸残基;Z5是选自由D和E组成的组的氨基酸残基;和Z6是选自由C、G和P组成的组的氨基酸残基。
一些优选的本发明的GAT多肽包含选自由如下组成的组的氨基酸序列:(a)与SEQ ID NO:577至少98%相同的氨基酸序列;(b)与SEQ ID NO:578至少97%相同的氨基酸序列;(c)与SEQ ID NO:621至少97%相同的氨基酸序列;(d)与SEQ ID NO:579至少98%相同的氨基酸序列;(e)与SEQ ID NO:602至少98%相同的氨基酸序列;(f)与SEQ ID NO:697至少95%相同的氨基酸序列;(g)与SEQ ID NO:712至少96%相同的氨基酸序列;(h)与SEQ ID NO:613至少97%相同的氨基酸序列;(i)与SEQ ID NO:677至少89%相同的氨基酸序列;(j)与SEQ ID NO:584至少95%相同的氨基酸序列;(k)与SEQ ID NO:707至少98%相同的氨基酸序列;(l)与SEQ ID NO:616至少98%相同的氨基酸序列;(m)与SEQ ID NO:612至少96%相同的氨基酸序列;和(n)与SEQ ID NO:590至少98%相同的氨基酸序列,其中氨基酸序列中对应于如下位置的氨基酸残基至少90%地遵从于如下限制:(a)在位置2、4、15、19、26、28、31、45、51、54、86、90、91、97、103、105、106、114、123、129、139和/或145上,氨基酸残基为B1;和(b)在位置3、5、8、10、11、14、17、24、27、32、37、47、48、49、52、57、58、61、63、68、69、79、80、82、83、89、92、100、101、104、119、120、125、126、128、131和/或143上,氨基酸残基为B2;其中B1为选自由A、I、L、M、F、W、Y和V组成的组的氨基酸;而B2是选自由R、N、D、C、Q、E、G、H、K、P、S和T组成的组的氨基酸。
一些优选的本发明的GAT多肽包含选自由如下组成的组的氨基酸序列:(a)与SEQ ID NO:577至少98%相同的氨基酸序列;(b)与SEQ ID NO:578至少97%相同的氨基酸序列;(c)与SEQ ID NO:621至少97%相同的氨基酸序列;(d)与SEQ ID NO:579至少98%相同的氨基酸序列;(e)与SEQ ID NO:602至少98%相同的氨基酸序列;(f)与SEQ ID NO:697至少95%相同的氨基酸序列;(g)与SEQ ID NO:712至少96%相同的氨基酸序列;(h)与SEQ ID NO:613至少97%相同的氨基酸序列;(i)与SEQ ID NO:677至少89%相同的氨基酸序列;(j)与SEQ ID NO:584至少95%相同的氨基酸序列;(k)与SEQ ID NO:707至少98%相同的氨基酸序列;(l)与SEQ ID NO:616至少98%相同的氨基酸序列;(m)与SEQ ID NO:612至少96%相同的氨基酸序列;和(n)与SEQ ID NO:590至少98%相同的氨基酸序列,其中氨基酸序列中对应于如下位置的氨基酸残基至少80%地遵从于如下限制:(a)在位置2、4、15、19、26、28、31、45、51、54、86、90、91、97、103、105、106、114、123、129、139和/或145上,氨基酸残基为Z1;和(b)在位置31和/或45上,氨基酸残基为Z2;(c)在位置8上,氨基酸残基为Z3;(d)在位置89上,氨基酸残基为Z3或者Z6;(e)在位置82,92,101和/或120上,氨基酸残基为Z4;(f)在位置3、11、27和/或79上,氨基酸残基为Z5;(g)在位置18上,氨基酸残基为Z4或Z5;(h)在位置123上,氨基酸残基为Z1或Z2;(i)在位置12、33、35、39、53、59、112、132、135、140和/或146上,氨基酸残基为Z1或Z3;(j)在位置30上,氨基酸残基为Z1;(k)在位置6上,氨基酸残基为Z6;(l)在位置81上,氨基酸残基为Z2或Z4;(m)在位置113上,氨基酸残基为Z3;(n)在位置138上,氨基酸残基为Z4;(o)在位置142上,氨基酸残基为Z2;(p)在位置57和/或126上,氨基酸残基为Z3或Z4;(q)在位置5,17和61上,氨基酸残基为Z4;(r)在位置24上,氨基酸残基为Z3;(s)在位置104上,氨基酸残基为Z5;(t)在位置52和/或69上,氨基酸残基为Z3;(u)在位置14和/或119上,氨基酸残基为Z5;(v)在位置10、32、63和/或83上,氨基酸残基为Z5;(w)在位置48和/或80上,氨基酸残基为Z6;(x)在位置40上,氨基酸残基为Z1或Z2;(y)在位置96上,氨基酸残基为Z3或Z5;(z)在位置65上,氨基酸残基为Z3、Z4或Z6;(aa)在位置84和/或115上,氨基酸残基为Z3;(ab)在位置93上,氨基酸残基为Z4;(ac)在位置130上,氨基酸残基为Z2;(ad)在位置58上,氨基酸残基为Z3、Z4或者Z6;(ae)在位置47上,氨基酸残基为Z4或Z6;(af)在位置49和/或100上,氨基酸残基为Z3或Z4;(ag)在位置68上,氨基酸残基为Z4或Z5;(ah)在位置143上,氨基酸残基为Z4;(ai)在位置131上,氨基酸残基为Z5;(aj)在位置125和/或128上,氨基酸残基为Z5;(ak)在位置67上,氨基酸残基为Z3或Z4;(al)在位置60上,氨基酸残基为Z5;和(am)在位置37上,氨基酸残基为Z4或Z6;其中Z1是选自由A、I、L、M和V组成的组的氨基酸残基;Z2是选自由F、W和Y组成的组的氨基酸残基;Z3是选自由N、Q、S和T组成的组的氨基酸;Z4是选自由R、H和K组成的组的氨基酸;Z5是选自由D和E组成的组的氨基酸残基;和Z6是选自由C、G和P组成的组的氨基酸残基。
一些优选的本发明的GAT多肽在对应于在(a)-(am)中指定的位置的氨基酸序列中还包含氨基酸残基,其中至少90%地遵从在(a)-(am)中指定的氨基酸残基的限制。
一些优选的本发明的GAT多肽在对应于如下位置的氨基酸序列中还包含氨基酸残基,其中至少90%地遵从如下的额外限制:(a)在位置1、7、9、13、20、36、42、46、50、56、64、70、72、75、76、78、94、98、107、110、117、118、121和/或141上,氨基酸残基为B1;和(b)在位置16、21、22、23、25、29、34、41、43、44、55、66、71、73、74、77、85、87、88、95、99、102、108、109、111、116、122、127、133、134、136和/或137上,氨基酸残基为B2;其中B1为选自由A、I、L、M、F、W、Y和V组成的组的氨基酸;而B2是选自由R、N、D、C、Q、E、G、H、K、P、S和T组成的组的氨基酸。
一些优选的本发明的GAT多肽在对应于如下位置的氨基酸序列中还包含氨基酸残基,其中至少90%地遵从如下的额外限制:(a)在位置1、7、9、13、20、36、42、46、50、56、64、70、72、75、76、78、94、98、107、110、117、118、121和/或141上,氨基酸残基为B1;和(b)在位置16、21、22、23、25、29、34、41、43、44、55、66、71、73、74、77、85、87、88、95、99、102、108、109、111、116、122、127、133、134、136和/或137上,氨基酸残基为B2;其中B1为选自由A、I、L、M、F、W、Y和V组成的组的氨基酸;而B2是选自由R、N、D、C、Q、E、G、H、K、P、S和T组成的组的氨基酸。
一些优选的本发明的GAT多肽在对应于如下位置的氨基酸序列中还包含氨基酸残基,其中至少90%地遵从如下限制:(a)在位置1、7、9、20、42、50、72、75、76、78、94、98、110、121和/或141上,氨基酸残基为Z1;(b)在位置13、46、56、70、107、117和/或118上,氨基酸残基为Z2;(c)在位置23、55、71、77、88和/或109上,氨基酸残基为Z3;(d)在位置16、21、41、73、85、99和/或111上,氨基酸残基为Z4;(e)在位置34和/或95上,氨基酸残基为Z5;(f)在位置22、25、29、43、44、66、74、87、102、108、116、122、127、133、134、136和/或137上,氨基酸残基为Z6;其中Z1是选自由A、I、L、M和V组成的组的氨基酸残基;Z2是选自由F、W和Y组成的组的氨基酸残基;Z3是选自由N、Q、S和T组成的组的氨基酸;Z4是选自由R、H和K组成的组的氨基酸;Z5是选自由D和E组成的组的氨基酸残基;和Z6是选自由C、G和P组成的组的氨基酸残基。
一些优选的本发明的GAT多肽还包含一种氨基酸序列,其中位置36上的氨基酸残基选自由Z1和Z3组成的组。一些本发明的优选GAT多肽还包含一种氨基酸序列,其中位置64上的氨基酸残基选自由Z1和Z2组成的组。
一些优选的本发明的GAT多肽还包含一种氨基酸序列,其中对应于如下位置的氨基酸残基至少80%地遵从如下限制:(a)在位置2上,氨基酸残基为I或L;(b)在位置3上,氨基酸残基为E;(c)在位置4上,氨基酸残基为V或I;(d)在位置5上,氨基酸残基为K;(e)在位置6上,氨基酸残基为P;(f)在位置8上,氨基酸残基为N;(g)在位置10上,氨基酸残基为E;(h)在位置11上,氨基酸残基为D或E;(i)在位置12上,氨基酸残基为T;(j)在位置14上,氨基酸残基为E或D;(k)在位置15上,氨基酸残基为L;(l)在位置17上,氨基酸残基为H;(m)在位置18上,氨基酸残基为R、E或K;(n)在位置19上,氨基酸残基为I或V;(o)在位置24上,氨基酸残基为Q;(p)在位置26上,氨基酸残基为M、L、V或I;(q)在位置27上,氨基酸残基为E;(r)在位置28上,氨基酸残基为A或V;(s)在位置30上,氨基酸残基为M;(t)在位置31上,氨基酸残基为Y或F;(u)在位置32上,氨基酸残基为E或D;(v)在位置33上,氨基酸残基为T或S;(w)在位置35上,氨基酸残基为L;(x)在位置37上,氨基酸残基为R、G、E或Q;(y)在位置39上,氨基酸残基为A或S;(z)在位置40上,氨基酸残基为F或L;(aa)在位置45上,氨基酸残基为Y或F;(ab)在位置47上,氨基酸残基为R或G;(ac)在位置48上,氨基酸残基为G;(ad)在位置49上,氨基酸残基为K、R或Q;(ae)在位置51上,氨基酸残基为I或V;(af)在位置52上,氨基酸残基为S;(ag)在位置53上,氨基酸残基为I或V;(ah)在位置54上,氨基酸残基为A;(ai)在位置57上,氨基酸残基为H或N;(aj)在位置58上,氨基酸残基为Q、K、R或P;(ak)在位置59上,氨基酸残基为A;(al)在位置60上,氨基酸残基为E;(am)在位置61上,氨基酸残基为H或R;(an)在位置63上,氨基酸残基为E或D;(ao)在位置65上,氨基酸残基为E、P或Q;(ap)在位置67上,氨基酸残基为Q或R;(aq)在位置68上,氨基酸残基为K或E;(ar)在位置69上,氨基酸残基为Q;(as)在位置79上,氨基酸残基为E;(at)在位置80上,氨基酸残基为G;(au)在位置81上,氨基酸残基为Y、H或F;(av)在位置82上,氨基酸残基为R;(aw)在位置83上,氨基酸残基为E或D;(ax)在位置84上,氨基酸残基为Q;(ay)在位置86上,氨基酸残基为A;(az)在位置89上,氨基酸残基为G、T或S;(ba)在位置90上,氨基酸残基为L;(bb)在位置91上,氨基酸残基为L、I或V;(bc)在位置92上,氨基酸残基为R或K;(bd)在位置93上,氨基酸残基为H;(be)在位置96上,氨基酸残基为E或Q;(bf)在位置97上,氨基酸残基为I;(bg)在位置100上,氨基酸残基为K或N;(bh)在位置101上,氨基酸残基为K或R;(bi)在位置103上,氨基酸残基为A或V;(bj)在位置104上,氨基酸残基为D;(bk)在位置105上,氨基酸残基为M、L或I;(Bl)在位置106上,氨基酸残基为L;(bm)在位置112上,氨基酸残基为T或A;(bn)在位置113上,氨基酸残基为S或T;(bo)在位置114上,氨基酸残基为A;(bp)在位置115上,氨基酸残基为S;(bq)在位置119上,氨基酸残基为K或R;(br)在位置120上,氨基酸残基为K或R;(bs)在位置123上,氨基酸残基为F或L;(bt)在位置125上,氨基酸残基为E;(bu)在位置126上,氨基酸残基为Q或H;(bv)在位置128上,氨基酸残基为E或D;(bw)在位置129上,氨基酸残基为V或I;(bx)在位置130上,氨基酸残基为F;(by)在位置131上,氨基酸残基为D或E;(bz)在位置132上,氨基酸残基为T;(ca)在位置135上,氨基酸残基为V;(cb)在位置138上,氨基酸残基为H;(cc)在位置139上,氨基酸残基为I;(cd)在位置140上,氨基酸残基为L或M;(ce)在位置142上,氨基酸残基为Y;(cf)在位置143上,氨基酸残基为K或R;(cg)在位置145上,氨基酸残基为L或I;和(ch)在位置146上,氨基酸残基为T。
一些优选的本发明的GAT多肽包含氨基酸序列,其中对应于在上面的(a)-(ch)中指出的位置的残基至少90%地遵从于在(a)-(ch)中指出的氨基酸残基的限制。
一些优选的本发明的GAT多肽包含选自由如下组成的组的氨基酸序列:(a)与SEQ ID NO:577至少98%相同的氨基酸序列;(b)与SEQ ID NO:578至少97%相同的氨基酸序列;(c)与SEQ ID NO:621至少97%相同的氨基酸序列;(d)与SEQ ID NO:579至少98%相同的氨基酸序列;(e)与SEQ ID NO:602至少98%相同的氨基酸序列;(f)与SEQ ID NO:697至少95%相同的氨基酸序列;(g)与SEQ ID NO:712至少96%相同的氨基酸序列;(h)与SEQ ID NO:613至少97%相同的氨基酸序列;(i)与SEQ ID NO:677至少89%相同的氨基酸序列;(j)与SEQ ID NO:584至少95%相同的氨基酸序列;(k)与SEQ ID NO:707至少98%相同的氨基酸序列;(l)与SEQ IDNO:616至少98%相同的氨基酸序列;(m)与SEQ ID NO:612至少96%相同的氨基酸序列;和(n)与SEQ ID NO:590至少98%相同的氨基酸序列,其中氨基酸序列中对应于如下位置的氨基酸残基至少80%地遵从于如下限制:(a)在位置9、76、94和110上,氨基酸残基为A;(b)在位置29和108上,氨基酸残基为C;(c)在位置34上,氨基酸残基为D;(d)在位置95上,氨基酸残基为E;(e)在位置56上,氨基酸残基为F;(f)在位置43、44、66、74、87、102、116、122、127和136上,氨基酸残基为G;(g)在位置41上,氨基酸残基为H;(h)在位置7上,氨基酸残基为I;(i)在位置85上,氨基酸残基为K;(j)在位置20、42、50、78和121上,氨基酸残基为L;(k)在位置1和141上,氨基酸残基为M;(l)在位置23和109上,氨基酸残基为N;(m)在位置22、25、133、134和137上,氨基酸残基为P;(n)在位置71上,氨基酸残基为Q;(o)在位置16、21、73、99和111上,氨基酸残基为R;(p)在位置55上,氨基酸残基为S;(q)在位置77上,氨基酸残基为T;(r)在位置107上,氨基酸残基为W;和(s)在位置13、46、70和118上,氨基酸残基为Y。
一些优选的本发明的GAT多肽还包含氨基酸序列,其中至少满足如下标准之一:(a)在位置14上,氨基酸残基为D;(b)在位置18上,氨基酸残基为E;(c)在位置26上,氨基酸残基为M或V;(e)在位置30上,氨基酸残基为I;(f)在位置32上,氨基酸残基为D;(g)在位置36上,氨基酸残基为M或T;(i)在位置37上,氨基酸残基为C;(j)在位置38上,氨基酸残基为D;(j)在位置53上,氨基酸残基为V;(k)在位置58上,氨基酸残基为R;(l)在位置61上,氨基酸残基为R;(m)在位置62上,氨基酸残基为L;(n)在位置64上,氨基酸残基为I或F;(o)在位置65上,氨基酸残基为P;(p)在位置72上,氨基酸残基为I;(q)在位置75上,氨基酸残基为V;(r)在位置88上,氨基酸残基为T;(s)在位置89上,氨基酸残基为G;(t)在位置91上,氨基酸残基为L;(u)在位置98上,氨基酸残基为I;(v)在位置105上,氨基酸残基为I;(w)在位置112上,氨基酸残基为A;(x)在位置124上,氨基酸残基为G或C;(y)在位置128上,氨基酸残基为D;(z)在位置140上,氨基酸残基为M;(aa)在位置143上,氨基酸残基为R;和(ab)在位置144上,氨基酸残基为W。
一些优选的本发明的GAT多肽还包含氨基酸序列,其中对应于在上面的(a)-(ab)中指定的位置的氨基酸残基至少80%地遵从在(a)-(ab)中指定的氨基酸残基的限制。
一些优选的本发明的GAT多肽还包含氨基酸序列,其中还满足如下条件:(a)在位置41上,氨基酸残基为H;(b)在位置138上,氨基酸残基为H;(c)在位置34上,氨基酸残基为N;和(d)在位置55上,氨基酸残基为S。
当一些优选的本发明的GAT多肽最佳地与选自由SEQ ID NO:300、445或457组成的组的参比序列比较时,采用B1OSUM62矩阵,存在一个缺口罚11分,缺口一个延伸罚1分,产生至少为460的相似性分值,GAT多肽具有一个或多个如下位置满足如下限制的氨基酸序列:(i)在位置18和38上,存在一个Z5氨基酸残基;(ii)在位置62上,存在一个Z1氨基酸残基;(iii)在位置124上,存在一个Z6氨基酸残基;和(iv)在位置144上,存在一个Z2氨基酸残基,其中:Z1是选自由A、I、L、M和V组成的组的氨基酸残基;Z2是选自由F、W和Y组成的组的氨基酸残基;Z5是选自由D和E组成的组的氨基酸残基;和Z6是选自由C、G和P组成的组的氨基酸残基。在前述GAT多肽的某些中,多肽中对应于位置28的氨基酸残基为V,I或A。位置28上的缬氨酸或异亮氨酸通常与KM降低有关,而该位置的丙氨酸通常与k催化升高有关。位置89上的苏氨酸和位置58上的精氨酸通常与KM降低有关。其他优选的GAT多肽被表征为具有127(即位置27上的I)、M30、D34、S35、R37、S39、H41、G48、K49、N57、Q58、P62、T62、Q65、Q67、K68、V75、E83、S89、A96、E96、R101、T112、A114、K119、K120、E128、V129、D131、T131、V132、V134、V135、H138、R144、1145或T146,或者它们的任意组合。
一些优选的本发明的GAT多肽包含选自由如下组成的组的氨基酸序列:SEQ ID NO:568、569、570、571、572、573、574、575、576、577、578、579、580、581、582、583、584、585、586、587、588、589、590、591、592、593、594、595、596、597、598、599、600、601、602、603、604、605、606、607、608、609、610、611、612、613、614、615、616、617、618、619、621、623、625、627、629、631、633、635、637、639、641、643、645、647、649、651、653、655、657、659、661、663、665、667、669、671、673、675、677、679、681、683、685、687、689、691、693、695、697、699、701、703、705、707、709、711、713、715、717、719、721、723、725、727、729、731、733、735、737、739、741、743、745、747、749、751、753、755、757、759、761、763、765、767、769、771、773、775、777、779、781、783、785、787、789、791、793、795、797、799、801、803、805、807、809、811、813、815、817、819、821、823和825。
另一个方面,本发明提供分离的或重组的多肽,其包含选自由如下组成的组的氨基酸序列中的至少20个,或者可替代地至少50个、至少75个、至少100个、至少125个、至少130个、至少135个、至少140个、至少141个、至少142个、至少143个、至少144或至少145个连续的氨基酸:(a)与SEQ ID NO:919至少96%相同的氨基酸序列(例如SEQ ID NO:917、919、921、923、925、927、833、835、839、843、845、859、863、873、877、891、895、901、905、907、913、915或950);(b)与SEQ ID NO:929至少97%相同的氨基酸序列(例如SEQ ID NO:929、931、835、843、849或867);(c)与SEQ ID NO:847至少98%相同的氨基酸序列(例如SEQ ID NO:845或847);(d)与SEQ ID NO:851至少98%的氨基酸序列;(e)与SEQ ID NO:853至少98%相同的氨基酸序列;(f)与SEQ ID NO:855至少98%相同的氨基酸序列(例如SEQ ID NO:835或855);(g)与SEQ ID NO:857至少98%相同的氨基酸序列;(h)与SEQ ID NO:861至少98%相同的氨基酸序列(例如SEQ ID NO:839、861或883);(i)与SEQ ID NO:871至少98%相同的氨基酸序列;(j)与SEQID NO:875至少98%相同的氨基酸序列;(k)与SEQ ID NO:881至少98%相同的氨基酸序列;(l)与SEQ ID NO:885至少98%相同的氨基酸序列(例如SEQ ID NO:845或885);(m)与SEQ ID NO:887至少98%相同的氨基酸序列;(n)与SEQ ID NO:889至少98%相同的氨基酸序列(例如,SEQ ID NO:863、889、891或903);(o)与SEQ ID NO:893至少98%相同的氨基酸序列;(p)与SEQ IDNO:897至少98%相同的氨基酸序列;(q)与SEQ ID NO:899至少98%相同的氨基酸序列;(r)与SEQ ID NO:909至少98%相同的氨基酸序列(例如,SEQ ID NO:883或909);(s)与SEQ ID NO:911至少98%相同的氨基酸序列;(t)与SEQ ID NO:837至少99%相同的氨基酸序列;(u)与SEQ ID NO:841至少99%相同的氨基酸序列;(v)与SEQ ID NO:865至少99%相同的氨基酸序列;(w)与SEQ IDNO:869至少99%相同的氨基酸序列;和(x)与SEQ ID NO:879至少99%相同的氨基酸序列。
另一个方面,本发明提供分离的或重组的多肽,该多肽包含与SEQID NO:929至少95%相同的氨基酸序列中的至少20个,或者可替代地至少50个、至少75个、至少100个、至少125个、至少130个、至少135个、至少140个、至少141个、至少142个、至少143个、至少144或至少145个连续的氨基酸,并且在对应于SEQ ID NO:929的位置33的氨基酸位置上包含Gly或者Asn残基(例如,SEQ ID NO:837、849、893、897、905、921、927、929或931)。
另一个方面,本发明提供包含选自由如下组成的组的氨基酸序列的残基2-146的多肽,SEQ ID NO:833、835、837、839、841、843、845、847、849、851、853、855、857、859、861、863、865、867、869、871、873、875、877、879、881、883、885、887、889、891、893、895、897、899、901、903、905、907、909、911、913、915、917、919、921、923、925、927、929、931、953、954、955、956、957、958、959、960、961、962、963、964、965、966、967、968、969、970、971和972。在本发明的一些实施方案中,多肽的氨基酸序列在对应于参比氨基酸序列的位置2的氨基酸的N末端一侧上包含Met、Met-Ala或Met-Ala-Ala。
一些优选的本发明的GAT多肽包含选自由如下组成的组的氨基酸序列:SEQ ID NO:833、835、837、839、841、843、845、847、849、851、853、855、857、859、861、863、865、867、869、871、873、875、877、879、881、883、885、887、889、891、893、895、897、899、901、903、905、907、909、911、913、915、917、919、921、923、925、927、929、931、946、948和950。
本发明还提供优选的GAT多肽,其特征为前述的氨基酸位置限制的组合。
此外,本发明提供编码上述的优选GAT多肽的GAT多核苷酸,及其互补核苷酸序列。
如在此所描述,本发明的某些方面特别地涉及具有GAT活性的GAT多肽的上述分类的任何之一的子集。这些GAT多肽是优选的,例如用作试剂、赋予植物以草甘膦抗性。GAT活性的期望水平的例子在此被描述。
在一个方面中,GAT多肽包含由重组的或者分离形式的天然存在的核苷酸所编码的氨基酸序列,分离形式的核苷酸从天然来源如细菌菌株中分离得到。用本领域已知的标准技术特异性地筛选编码这种GAT多肽的野生型的多核苷酸。通过表达来自具有GAT活性的杆菌菌株的序列克隆发现了SEQ ID NO:6-10所定义的多肽,这将在下文被更加详细地描述。
本发明还包括分离的或重组的多肽,其由包含核苷酸序列的分离的或重组的多核苷酸所编码,该核苷酸序列在严紧条件下可以与几乎全长的选自由SEQ ID NO:516、517、518、519、520、521、522、523、524、525、526、527、528、529、530、531、532、533、534、535、536、537、538、539、540、541、542、543、544、545、546、547、548、549、550、551、552、553、554、555、556、557、558、559、560、561、562、563、564、565、566、567、620、622、624、626、628、630、632、634、636、638、640、642、644、646、648、650、652、654、656、658、660、662、664、666、668、670、672、674、676、678、680、682、684、686、688、690、692、694、696、698、700、702、704、706、708、710、712、714、716、718、720、722、724、726、728、730、732、734、736、738、740、742、744、746、748、750、752、754、756、758、760、762、764、768、770、772、774、776、778、780、782、784、786、788、790、792、794、796、798、800、802、804、806、808、810、812、814、816、818、820、822和824组成的组的核苷酸序列及其补体杂交,以及与全长的编码选自由SEQ ID NO:568、569、570、571、572、573、574、575、576、577、578、579、580、581、582、583、584、585、586、587、588、589、590、591、592、593、594、595、596、597、598、599、600、601、602、603、604、605、606、607、608、609、610、611、612、613、614、615、616、617、618、619、621、623、625、627、629、631、633、635、637、639、641、643、645、647、649、651、653、655、657、659、661、663、665、667、669、671、673、675、677、679、681、683、685、687、689、691、693、695、697、699、701、703、705、707、709、711、713、715、717、719、721、723、725、727、729、731、733、735、737、739、741、743、745、747、749、751、753、755、757、759、761、763、765、767、769、771、773、775、777、779、781、783、785、787、789、791、793、795、797、799、801、803、805、807、809、811、813、815、817、819、821、823和825组成的组的氨基酸序列的核苷酸序列,包括与其补体杂交。
本发明还包括由分离的或重组的多核苷酸编码的分离的或重组的多肽,该多核苷酸包含核苷酸序列,该核苷酸序列在严紧条件下可以与几乎全长的选自由SEQ ID NO:832、834、836、838、840、842、844、846、848、850、852、854、856、858、860、862、864、866、868、870、872、874、876、878、880、882、884、886、888、890、892、894、896、898、900、902、904、906、908、910、912、914、916、918、920、922、924、926、928和930组成的组的核苷酸序列及其补体,以及编码选自由SEQ ID NO:833、835、837、839、841、843、845、847、849、851、853、855、857、859、861、863、865、867、869、871、873、875、877、879、881、883、885、887、889、891、893、895、897、899、901、903、905、907、909、911、913、915、917、919、921、923、925、927、929、931、946、948、950、953、954、955、956、957、958、959、960、961、962、963、964、965、966、967、968、969、970、971和972组成的组的氨基酸序列的核苷酸序列杂交。
本发明还包括任何具有GAT活性的多肽,该多肽由在此描述的编码GAT的多核苷酸的任何之一的片段所编码。
本发明还提供可以被一起剪接来构成功能性GAT多肽的GAT多肽的片段。剪接可以在体外或者体内进行,包括顺式或反式剪接(即分子外或分子内剪接)。片段本身可以具有,但是不是必需具有GAT活性。例如,两个或多个GAT多肽的区段被内含子分离;通过顺式剪接去除该内含子序列,生成功能性的GAT多肽。在另一个实施例中,密码子化的GAT多肽被表达成两种或多种分离的片段;反式剪接这些区段,导致恢复为一种功能性的GAT多肽。美国专利申请09/517,933和09/710,686中更加详细地描述了顺式和反式剪接、基因编码和间插序列的插入的各个方面,这两份申请在此完全引入作为参考。
总之,本发明包括由被修饰的GAT多核苷酸所编码的任何多肽,该被修饰的多核苷酸通过在此描述的多核苷酸序列的突变、递推序列重组和/或多样化来获得。在本发明的某些方面,通过单个或多个氨基酸取代、缺失、插入或者一种或多种这种类型的修饰的组合,来修饰GAT多肽。取代可以是保守的或者不保守的,可以改变功能或者不改变功能,以及可以添加新功能。插入和缺失可以是实质性的,例如截短序列的实质性片段,或者在内部或者在N末端或C末端融合任何其他序列。在本发明的一些实施方案中,GAT多肽是融合蛋白的一部分,融合蛋白包括功能性添加,例如分泌信号、叶绿体转运肽、纯化标记或者大量其他功能性基团的任何一种,这些对于本领域技术人员来说是显而易见的,本说明书在别处对此有更加详细的描述。
本发明的多肽可以含有一个或者多个被修饰的氨基酸。存在被修饰的氨基酸可能有利于,例如(a)延长多肽的体内半衰期,(b)降低或提高多肽的抗原性,和(c)提供多肽的存储稳定性。在重组生产的翻译过程中或者翻译后,氨基酸被修饰(例如在哺乳动物细胞中表达的过程中,在N-X-S/T基序处发生N连接的糖基化),或者通过合成手段修饰。
被修饰的氨基酸的非限制性例子包括糖基化的氨基酸、硫酸化的氨基酸、异戊二烯基化(例如法尼基化、香叶基化)的氨基酸、乙酰化的氨基酸、酰化的氨基酸、PEG化的氨基酸、生物素化的氨基酸和羧基化的氨基酸、磷酸化的氨基酸等。有很多参考文献可以指导熟练的技术人员如何修饰氨基酸。示例性的方法可见Walker(1998)ProteinProtocols on CD-ROM(Humana出版社,Towata,新泽西)。
用于生产和分离本发明的GAT多肽的重组方法在此被描述。除了重组生产外,可以用固相技术直接合成肽来生产此多肽(例如,Stewart等人(1969)Solid-Phase Peptide Synthesis(WH Freeman公司,旧金山)和Merrifield(1963)J.Am.Chem.Soc.85:2149-2154)。可以用人工技术或者自动化进行肽合成。自动化合成可以例如用Applied Biosystems 431A肽合成仪(Perkin Elmer,福斯特城,CA),按照生产商提供的说明书来进行。例如,可以分别地化学合成亚序列,并且用化学方法进行组合,来提供全长的GAT多肽。这些肽还可以从各种来源订购。
在本发明的另一个方面,用本发明的GAT多肽来生产抗体,这些抗体具有诊断用途,例如,涉及GAT多肽在转基因植物的各种组织中的活性、分布和表达。
用于抗体诱导的GAT同源多肽不必具有生物学活性;但是,该多肽或者寡肽必须具有抗原性。用于诱导特定的抗体的肽可以具有由至少10个氨基酸,优选至少15各个或20个氨基酸组成的氨基酸序列。短的GAT多肽链与另一种蛋白质,例如匙孔血蓝蛋白融合,产生抗嵌合分子的抗体。
生产多克隆和单克隆抗体的方法是本领域技术人员熟知的,许多抗体是可获得的。参见例如,Coligan(1991)Current Protocols inImmunology(Wiley/Greene,纽约州);Harlow和Lane(1989)Antibodies:A Laboratory Manual(冷泉巷出版社,纽约州);Stites等人(编辑)Basic and Clinical Immunolo,第4版(Lange MedicalPuBlications,Los Altos,加利福尼亚州),以及其中引用的文献;Goding(1986)Monoclinal Antibodies:Principles and Practice,第2版(学术出版社,纽约,纽约州);以及Kohler和Milstein(1975)Nature 256:495-497。其他适合抗体制备的技术包括在噬菌体或类似载体中筛选重组抗体的文库。参见Huse等人(1989)Science 246:1275-1281和Ward等人(1989)Nature 341:544-546。特异性的单克隆和多克隆抗体和抗血清通常以至少约0.1μM,优选地至少约0.01μM或更小,最典型地和优选地为0.001μM或更小的KD结合。
其他详细的抗体生产和工程化技术可见Borrebaeck编辑(1995)Antibody Engineering,第2版(Freeman和Company,纽约州);McCafferty等人(1996)Antibody Engineering,A PracticalApproach(IRL牛津出版社,牛津,英格兰);和Paul(1995)AntibodyEngineering Protocols(Humana Press,Towata,新泽西)。
序列变体
本发明的GAT多肽包括在此以SEQ ID NO:568、569、570、571、572、573、574、575、576、577、578、579、580、581、582、583、584、585、586、587、588、589、590、591、592、593、594、595、596、597、598、599、600、601、602、603、604、605、606、607、608、609、610、611、612、613、614、615、616、617、618、619、621、623、625、627、629、631、633、635、637、639、641、643、645、647、649、651、653、655、657、659、661、663、665、667、669、671、673、675、677、679、681、683、685、687、689、691、693、695、697、699、701、703、705、707、709、711、713、715、717、719、721、723、725、727、729、731、733、735、737、739、741、743、745、747、749、751、753、755、757、759、761、763、765、767、769、771、773、775、777、779、781、783、785、787、789、791、793、795、797、799、801、803、805、807、809、811、813、815、817、819、821、823、825、833、835、837、839、841、843、845、847、849、851、853、855、857、859、861、863、865、867、869、871、873、875、877、879、881、883、885、887、889、891、893、895、897、899、901、903、905、907、909、911、913、915、917、919、921、923、925、927、929、931、946、948、950、953、954、955、956、957、958、959、960、961、962、963、964、965、966、967、968、969、970、971和972形式公开的序列的保守性修饰的变异。这种保守性修饰的变异包含取代、添加或者缺失,其改变、添加或者缺失了SEQ ID NO:568、569、570、571、572、573、574、575、576、577、578、579、580、581、582、583、584、585、586、587、588、589、590、591、592、593、594、595、596、597、598、599、600、601、602、603、604、605、606、607、608、609、610、611、612、613、614、615、616、617、618、619、621、623、625、627、629、631、633、635、637、639、641、643、645、647、649、651、653、655、657、659、661、663、665、667、669、671、673、675、677、679、681、683、685、687、689、691、693、695、697、699、701、703、705、707、709、711、713、715、717、719、721、723、725、727、729、731、733、735、737、739、741、743、745、747、749、751、753、755、757、759、761、763、765、767、769、771、773、775、777、779、781、783、785、787、789、791、793、795、797、799、801、803、805、807、809、811、813、815、817、819、821、823、825、833、835、837、839、841、843、845、847、849、851、853、855、857、859、861、863、865、867、869、871、873、875、877、879、881、883、885、887、889、891、893、895、897、899、901、903、905、907、909、911、913、915、917、919、921、923、925、927、929、931、946、948、950、953、954、955、956、957、958、959、960、961、962、963、964、965、966、967、968、969、970、971和972的任何之一中的单个氨基酸或者一小百分数的氨基酸(典型地少于约5%,更加典型地少于约4%、2%或1%)。
例如,在此被确定为SEQ ID NO:6的146个氨基酸的多肽的保守性修饰的变异(例如缺失)将具有至少140个氨基酸的长度,优选至少141个氨基酸,更加优选至少144个氨基酸,以及更加优选至少145个氨基酸,对应于多肽序列的小于约5%、4%、2%或约1%或者更少的缺失。
根据在表2中列举的六个取代组,在此被确定为SEQ ID NO:6的多肽的保守性修饰变异的另一个例子(例如“保守性替代变体”),在该146个氨基酸多肽的最多达约7个残基(即少于约5%)上含有“保守性替代”。
本发明的GAT多肽的同源物,包括保守性替代的序列,可以作为较大的多肽的一部分出现,例如存在于GAT多肽中,GAT多肽为与信号序列融合的GAT融合物形式,信号序列例如为叶绿体靶向序列,或者在添加一个或多个用于蛋白质纯化的结构域(例如多聚组氨酸区段,FLAG标记区段等)后以较大的多肽的一部分出现。在后一种情况下,额外的结构域对蛋白质的GAT部分的活性影响很小或者无影响,而额外的结构域可以通过合成后的加工步骤去除,例如通过用蛋白酶处理而去除。
通过免疫反应确定多肽
由于本发明的多肽提供一类新的具有确定活性的酶,即能够乙酰化和酰化草甘膦,该多肽还提供例如可以在免疫学分析中被识别的新的结构特征。产生能够特异性地结合本发明的多肽的抗血清以及被该血清结合的多肽,都是本发明的特征。
本发明包括与抗体或者抗血清特异地结合或者特异地免疫反应的GAT多肽,生成的抗体或抗血清抵抗一种免疫原,该免疫原包含选自SEQ ID NO:568、569、570、571、572、573、574、575、576、577、578、579、580、581、582、583、584、585、586、587、588、589、590、591、592、593、594、595、596、597、598、599、600、601、602、603、604、605、606、607、608、609、610、611、612、613、614、615、616、617、618、619、621、623、625、627、629、631、633、635、637、639、641、643、645、647、649、651、653、655、657、659、661、663、665、667、669、671、673、675、677、679、681、683、685、687、689、691、693、695、697、699、701、703、705、707、709、711、713、715、717、719、721、723、725、727、729、731、733、735、737、739、741、743、745、747、749、751、753、755、757、759、761、763、765、767、769、771、773、775、777、779、781、783、785、787、789、791、793、795、797、799、801、803、805、807、809、811、813、815、817、819、821、823、825、833、835、837、839、841、843、845、847、849、851、853、855、857、859、861、863、865、867、869、871、873、875、877、879、881、883、885、887、889、891、893、895、897、899、901、903、905、907、909、911、913、915、917、919、921、923、925、927、929、931、946、948、950、953、954、955、956、957、958、959、960、961、962、963、964、965、966、967、968、969、970、971和972中的一种或多种的氨基酸序列。为了消除与其他GAT同源物的交叉反应,用可获得的相关蛋白来减除(subtracted)抗体或抗血清,这些相关蛋白例如为对应于在本申请申请日可获得的GenBank登录号的蛋白质或者多肽所代表的,以CAA70664、Z99109和Y09476为例。当登录号对应于一种核酸时,产生由核酸编码的多肽,并用于减除抗体/抗血清的目的。附图3列举了示例性GAT序列和在Genbank中可获得的最相关的序列YitI之间的相对同一性。天然的YitI的功能还未被阐明,但是该酶被证明具有可检测的GAT活性。
在一种典型的形式中,免疫分析使用多克隆抗血清,这种血清可以抵抗一种或多种多肽,该多肽包含一条或多条对应于SEQ ID NO:568、569、570、571、572、573、574、575、576、577、578、579、580、581、582、583、584、585、586、587、588、589、590、591、592、593、594、595、596、597、598、599、600、601、602、603、604、605、606、607、608、609、610、611、612、613、614、615、616、617、618、619、621、623、625、627、629、631、633、635、637、639、641、643、645、647、649、651、653、655、657、659、661、663、665、667、669、671、673、675、677、679、681、683、685、687、689、691、693、695、697、699、701、703、705、707、709、711、713、715、717、719、721、723、725、727、729、731、733、735、737、739、741、743、745、747、749、751、753、755、757、759、761、763、765、767、769、771、773、775、777、779、781、783、785、787、789、791、793、795、797、799、801、803、805、807、809、811、813、815、817、819、821、823、825、833、835、837、839、841、843、845、847、849、851、853、855、857、859、861、863、865、867、869、871、873、875、877、879、881、883、885、887、889、891、893、895、897、899、901、903、905、907、909、911、913、915、917、919、921、923、925、927、929、931、946、948、950、953、954、955、956、957、958、959、960、961、962、963、964、965、966、967、968、969、970、971和972或其亚序列(即,所提供的全长序列的至少约30%)的一个或多个的序列。来源于SEQ ID NO:568、569、570、571、572、573、574、575、576、577、578、579、580、581、582、583、584、585、586、587、588、589、590、591、592、593、594、595、596、597、598、599、600、601、602、603、604、605、606、607、608、609、610、611、612、613、614、615、616、617、618、619、621、623、625、627、629、631、633、635、637、639、641、643、645、647、649、651、653、655、657、659、661、663、665、667、669、671、673、675、677、679、681、683、685、687、689、691、693、695、697、699、701、703、705、707、709、711、713、715、717、719、721、723、725、727、729、731、733、735、737、739、741、743、745、747、749、751、753、755、757、759、761、763、765、767、769、771、773、775、777、779、781、783、785、787、789、791、793、795、797、799、801、803、805、807、809、811、813、815、817、819、821、823、825、833、835、837、839、841、843、845、847、849、851、853、855、857、859、861、863、865、867、869、871、873、875、877、879、881、883、885、887、889、891、893、895、897、899、901、903、905、907、909、911、913、915、917、919、921、923、925、927、929、931、946、948、950、953、954、955、956、957、958、959、960、961、962、963、964、965、966、967、968、969、970、971和972的可能的多肽免疫原的整个集合在下文被统称为“免疫原性多肽”。最佳地选择生成的抗血清,其与其他相关序列交叉反应的活性低,在免疫分析中使用多克隆抗血清之前,用一种或多种相关序列进行免疫吸收,来除去任何这种交叉反应活性。
为了生产用于免疫分析中的抗血清,如在此所描述,生产和纯化一种或多种免疫原性多肽。例如,可以在细菌的细胞系中生产重组蛋白。用免疫原性蛋白质结合标准佐剂,例如Freund佐剂,采用标准的小鼠免疫方法(参见,Harlow和Hane(1988),Antibodies,AlaboratoryManual(冷泉巷出版社,纽约),关于抗体生产、用于确定特定免疫反应活性的免疫分析形式和条件的标准描述),免疫小鼠的近交系(用于该分析是由于这种小鼠实质上在遗传上是相同的,结果可重复)。可替代地,将来源于在此公开的一种或多种合成的或重组的多肽与载体蛋白相偶联,用作免疫原。
收集多克隆抗血清,并在免疫分析中,例如在使用固定在固体支持物上的一种或多种免疫原性蛋白的固相免疫分析中,测定抗血清抵抗免疫原性多肽的效价。选择效价为106或更高的多克隆抗血清,汇集并用相关多肽减除,以生产减除的、汇集的、效价确定的多克隆抗血清,相关多肽如所提及的从GENBANK中确定的那些多肽。
检测减除的、汇集的、效价确定的多克隆抗血清抵抗相关多肽的交叉反应活性。优选地,至少两种免疫原性GAT被用于该确定中,优选与至少两种相关多肽连接,以确定被免疫原性多肽特异性地结合的抗体。
在这种比较分析中,确定用于减除的、汇集的、效价确定的多克隆抗血清的差别性的结合条件,与相关多肽的结合相比,效价确定的多克隆抗血清与免疫原性GAT多肽的结合信噪比至少高约5-10倍。也就是说,通过加入非特异性的竞争物如白蛋白或者脱脂奶粉,或者调节盐条件、温度等,可以调节此结合反应的严紧性。将这些结合条件用于随后的分析中,来确定测试的多肽是否被汇集的、效价确定的多克隆抗血清特异地结合。特别地,在差别性结合条件下,信噪比至少高出对照多肽2-5倍,并且与免疫原性多肽相比,显示至少约1/2信噪比的测试多肽与已知的GAT相比,与免疫原性多肽具有实质上的结构相似性,并且因此属于本发明的多肽。
在另一个实施例中,采用竞争结合形式的免疫分析被用于检测测试多肽。例如,如所指出,用对照GAT多肽进行免疫吸收,从汇集的抗血清混合物中去除交叉反应的抗体。然后将免疫原性多肽固定在固体支持物上,将该固体支持物暴露在减除的、汇集的抗血清中。往分析中加入测试蛋白来竞争与汇集的减除的抗血清的结合。测试蛋白相对于固定蛋白竞争与汇集的、减除的抗血清的结合的能力,与加入到分析中的免疫原性多肽竞争结合的能力相比较(免疫原性多肽有效地与固定的免疫原性多肽竞争,以结合到汇集的抗血清上)。采用标准的计算方法,计算出测试蛋白的交叉反应性的百分率。
在平行分析中,任选地测定对照蛋白竞争与汇集的、减除的抗血清结合的能力,与免疫原性多肽竞争与抗血清结合的能力相比较。此外,用标准的计算方法,计算出对照多肽的交叉反应百分率。当测试多肽的交叉反应百分率至少高5-10倍时,该测试多肽被认为特异性地结合汇集的、减除抗血清。
总之,免疫吸收和汇集的抗血清可用于在此描述的竞争性结合免疫分析中,将任何的测试多肽与免疫原性多肽比较。为了进行这种比较,在大的浓度范围内,分别分析两种多肽,用标准技术确定各种多肽抑制50%的减除的抗血清与固定蛋白结合的所需含量。如果所需的测试多肽的含量少于所需的免疫原性多肽含量的两倍,则认为测试多肽特异地结合由免疫原性多肽产生的抗体,条件是该含量至少高于对照多肽约5-10倍。
作为特异性的最终确定,任选地用免疫原性多肽(而不是对照多肽)完全地免疫吸收汇集的抗血清,直到几乎没有或者不能检测到减除的、汇集的抗血清与免疫原性多肽的结合。然后,检测这种被完全免疫吸收的抗血清与测试多肽的反应活性。如果只观察到一点点或者没有观察到反应活性(即所现察到的完全免疫吸收的抗血清与免疫原性多肽结合的信噪比不超过2倍),则测试多肽被该免疫原性蛋白诱导产生的抗血清特异性地结合。
草甘膦-N-乙酰转移酶多核苷酸
在一个方面中,本发明提供在此被称作为“草甘膦-N-乙酰转移酶的多核苷酸”或者“GAT多核苷酸”的新的分离的或重组的多核苷酸家族。GAT多核苷酸序列被表征为能够编码GAT多肽。总之,本发明包括编码在此描述的新GAT多肽的任何一种的任何核苷酸序列。在本发明的一些方面,编码具有GAT活性的GAT多肽的GAT多核苷酸是优选的。
在一个方面中,GAT多核苷酸包括从生物体如细菌菌株分离的天然存在的核酸的重组或分离形式。通过表达来自具有GAT活性的杆菌菌株的序列克隆,发现了示例性的GAT多核苷酸如SEQ ID NO:1-5。简而言之,对约500种杆菌和假单胞菌菌株的集合筛选N-乙酰化草甘膦的天然能力。菌株在LB中生长过夜,通过离心收获,在稀释的甲苯中渗透,然后洗涤并重悬在含有缓冲液、5mM草甘膦和200μM乙酰CoA的反应混合物中。将细胞在反应化合物中培养1-48小时,此时往反应中加入等量甲醇。然后在通过母离子模式质谱分析之前,离心沉淀细胞,过滤上清液。如附图2中所示,通过将反应混合物的质谱图与N-乙酰草甘膦标准物比较,确定反应产物为N-乙酰草甘膦阳性。产品检测依赖于两种底物(乙酰CoA和草甘膦),并且可以用加热变性细菌细胞来消除。
然后通过功能性筛选,从被确定的菌株中克隆单个的GAT多核苷酸。制备基因组DNA,用Sau3A1酶部分地消化。将约4kb的片段克隆到大肠杆菌表达载体中,并转化到电感受态大肠杆菌中。按照先前所述的反应,通过质谱分析确定各种具有GAT活性的克隆,但是用PMBS渗透代替甲苯洗涤。然后对基因组片段进行测序,并确定假定的GAT多肽编码开放阅读框。通过在大肠杆菌中表达开放的阅读框,检测从反应混合物生产的高N-乙酰草甘膦水平,证实了GAT基因的身份。
在本发明的另一个方面中,通过多样化来制备GAT多核苷酸,例如重组和/或突变一个或多个天然存在的、分离的或重组的GAT多核苷酸。如本文它处更加详细描述的那样,通常可以产生多样化的编码具有较高功能性的GAT多肽的GAT多核苷酸,与用作多样化加工中的底物或者亲本的GAT多核苷酸相比,该GAT多核苷酸表达的GAT多肽的催化功能、稳定性和表达水平更高。
本发明的多核苷酸具有各种用途,例如:重组生产(即表达)本发明的GAT多肽;作为转基因(例如给转基因植物赋予除草剂抗性);作为转化和质粒保养的可选择标记;作为免疫原;作为检测互补的或者部分互补的核酸的可选择标记(包括检测天然的GAT编码核酸);作为进一步产生多样性的底物,如重组反应或者突变反应来生产新的和/或改良的GAT同源物等。
值得一提的是,GAT多核苷酸的某些特定的、实质性的和可信的用途不要求该多核苷酸编码具有实质性的GAT活性的多肽。例如不编码活性酶的GAT多核苷酸可以是亲本多核苷酸的有价值来源,用于多样化过程中,来获得具有期望功能特性(例如高k催化或者k催化/Km、低Km、对热或其他环境因子的高稳定性、高的转录或翻译速度、对蛋白质水解断裂的抗性、减弱的抗原性等)的GAT多核苷酸变体,或非GAT多核苷酸。例如,在DNA改组试验中,将编码只有很少或者没有可检测活性的蛋白酶变体的核苷酸序列用作亲本多核苷酸,生成编码高活性蛋白酶的后代(Ness等人(1999)Nature Biotech.17:893-96)。
通过多样性产生方法或者递推序列重组(“RSR”)方法(例如,DNA改组)生产的多核苷酸序列是本发明的一个特征。使用在此描述的核酸的突变和重组方法是本发明的一个特征。例如,一种本发明的方法包括递推重组一种或多种上下文描述的本发明的核苷酸序列。重组步骤任选地在体内、离体、计算机芯片内或体外进行。这种多样性后代或者递推序列重组产生至少一个重组修饰的GAT多核苷酸的文库。本发明包括这种文库的成员所编码的多肽。
还包括多核苷酸的用途,多核苷酸在此也被称作为寡核苷酸,典型地具有至少12个碱基,优选至少15个、更加优选至少20个、30个或50个或者更多的碱基,其在严紧或高度严紧条件下与GAT多核苷酸序列杂交。按照在此指出的方法,该多核苷酸可被用作探针、引物、有义和反义试剂等。
根据本发明,GAT多核苷酸,包括编码GAT多肽、GAT多肽片段、相关的融合蛋白或其功能性等价物的核苷酸序列,被用于DNA重组分子中,该重组分子在合适的宿主细胞如细菌或植物细胞中指导GAT多肽的表达。由于遗传密码的内在简并性,编码实质上相同或者功能性等价的氨基酸序列的其他核酸序列也被用于克隆和表达GAT多核苷酸。
本发明提供编码转录和/或翻译产物的GAT多核苷酸,其随后被剪接并最终生成功能性的GAT多肽。剪接可在体外或者体内实现,包括顺式或反式剪接。用于剪接的底物可以是多核苷酸(例如RNA转录)或者多肽。多核苷酸顺式剪接的例子是将插入编码序列的内含子去除,两个侧面连接的外显子区域被剪接,生成GAT多肽的编码序列。反式剪接的例子为将编码序列分成两个或多个可以分别转录的片段,然后将它们拼接成全长的GAT编码序列。剪接增强子序列(可以被导入本发明的构建体中)的应用便于顺式或反式剪接。多肽的顺式和反式剪接将在下文别处和美国专利申请09/517,933和09/710,686中被更加详细地描述。
因此,某些GAT多核苷酸不直接编码全长的GAT多肽,而是编码一个或几个GAT多肽的片段。通过与剪接相关的机制,可以用这些GAT多核苷酸表达功能性GAT多肽,其中剪接可以在多核苷酸(例如内含子/外显子)和/或多肽(例如intein/extein)的水平上进行。例如,在控制GAT活性的表达中,这是有用的,因为仅所有所需的片段在允许剪接加工产生功能性产物的条件下被表达时,才表达功能性GAT多肽。在另一个实施例中,将一条或多条序列导入GAT多核苷酸中,便于与低同源性的多肽发生重组;用内含子或内含肽来插入序列,便于去除间插序列,从而保留所编码的变体的功能。
本领域技术人员将理解,修饰编码序列以增强其在特定宿主中的表达是有利的。遗传密码富余64个密码子,但是大部分生物体优先使用这些密码子的一部分。在物种中最常使用的密码子被称作为最优密码子,而那些不常使用的密码子被列为稀有或者低利用度密码子(参见例如,Zhang等人(1991)Gene 105:61-72)。可以进行密码子取代来反映宿主的优先使用的密码子,这个过程有时被称作为“密码子优化”或者“对物种密码子偏爱的控制”。
制备含有特定原核或者真核宿主所优选的密码子的优化编码序列(还可以参见Murray等人(1989)Nucl.Acids Res.17:477-508),来提高翻译速度或者产生具有期望特性的重组RNA转录本,例如,与从未优化的序列产生的转录本相比,该转录本的半衰期更长。也可以修饰翻译终止密码子来反映宿主性能。例如,酿酒酵母和哺乳动物的优选终止密码子分别为UAA和UGA。单子叶植物的优选终止密码子为UGA,而昆虫和大肠杆菌偏爱使用UAA作为终止密码子(Dalphin等人(1996)Nucl.Acids Res.24:216-218)。用于优化在植物中表达的核苷酸序列的方法在美国专利6,015,891及其引用的参考文献中提供。
本发明的一个实施方案包括一种GAT多核苷酸,其具有用于在相关宿主如转基因植物宿主中表达的最佳密码子。当细菌来源的GAT多核苷酸被导入转基因植物中,例如用于给该植物赋予草甘膦抗性时,特别期望这种多核苷酸。
本发明的多核苷酸序列被工程化以改变GAT多核苷酸,包括但是不限于改变该基因产物的克隆、加工和/或表达的变化。例如,用本领域熟知的方法如定点诱变来引入变化、插入新的限制性位点、改变糖基化模式、改变密码子偏爱、引入剪接位点等。
如在此更加详细的描述,本发明的多核苷酸包括编码新GAT多肽的序列和与该编码序列互补的序列以及编码序列的新片段及其补体。多核苷酸可以是RNA的形式或者DNA形式,包括mRNA、cRNA、合成的RNA和DNA、基因组DNA和cDNA。多核苷酸可以是双链的或单链的,如果为单链的,可以是编码链或者非编码(反义的,互补的)链。多核苷酸任选地包括GAT多肽的编码序列,可以是(i)分离的,(ii)与其他编码序列重组,以编码例如融合蛋白、前体蛋白、前原蛋白等,(iii)与非编码序列,例如内含子或内含肽、控制序列如启动子、增强子、终止元件或对于编码序列在合适宿主中的表达有效的5′和/或3′非翻译区组合,和/或(iv)在载体或宿主环境中,其中GAT多核苷酸为一种异源基因。还可以发现该序列与典型的核酸组合物制剂组合,包括在载体、缓冲液、佐剂、赋形剂等的存在下。
可以按照已知的合成方法,通过标准的固相技术,来制备本发明的多核苷酸和寡核苷酸。典型地,分别合成长达100个碱基的片段,然后连接(例如通过酶催化或者化学连接方法或者聚合酶介导的方法)形成任何实际期望的连续序列。例如,通过化学合成来制备本发明的多核苷酸和寡核苷酸,采用例如在Beaucage等人(1981)TetrahedronLetters 22:1859-6中描述的经典氨基亚磷酸酯法或者由Matthes等人(1984)EMBO J.3:801-05中描述的方法,例如通常在自动合成方法中使用的方法。按照该氨基亚磷酸酯方法,合成寡核苷酸,例如在自动DNA合成仪中合成、纯化、退火、连接和克隆到载体中。
此外,实际上任何核酸可以向众多商业来源之一订购,例如TheMidland Certified Reagent Company(mcrc@oligos.com)、The GreatAmerican Gene Company(www.genco.com)、ExpressGen公司(www.expressgen.com)、Operon Technologies公司(Alameda,CA)和许多其他公司。类似地,肽和抗体可以向众多商业来源之一订购,例如PeptidoGenic(pkim@ccnet.com)、HTI Bio-products公司(www.htibio.com)、BMA Biomedicals Ltd(英国)、Bio.Synthesis公司和许多其他公司。
还可以用技术文献中描述的公知技术合成多核苷酸。参见,例如Carruthers等人,Cold Spring Harbor symp.Quant.Biol.47:411-418(1982)和Adams等人(1983)J Am.Chem.Soc.105:661。然后通过合成互补链并在合适条件下将互补链退火,或者通过用合适的引物序列和DNA聚合酶仪器加入互补链,来获得双链的DNA片段。
描述可用于此的分子生物学技术包括诱变的普通教课书,包括Berger和Kimmel,Guide to Molecular Cloning Techniques,Methods in Enzyniology,第152卷(学术出版社公司,圣地亚哥,加利福尼亚州);Sambrook等人(1989)Molecular Cloning-ALaboratory Manual,第2版,第1-3卷(冷泉巷实验室,冷泉巷,纽约);和Ausubel等人编辑(2000)Current Protocols in MolecularBiology(Greene PuBlishing Associates公司和John Wiley & Sons公司)。足以引导技术人员实施体外扩增方法的例子,包括聚合酶链式反应(PCR)、连接酶链式反应(LCR)、Qβ-复制酶扩增和其他RNA聚合酶介导的技术(例如,NASBA),可见于Berger,Sambrook,和Ausubel,以及Mullis等人(1987)美国专利4,683,202;Innis等人编辑(1990)PCR Protocols:A Guide to Methods andApplications(学术出版社公司,圣地亚哥,加利福尼亚州);Arnheim& Levinson(1990年10月1日)Chemical and Engineering News36-47;The Jourfzal Of NIH Research(1991)3:81-94;Kwoh等人(1989)Proc.Nat′l.Acad.Sci.USA 86:1173;Guatelli等人(1990)Proc.Nat′l.Acad.Sci.USA 87:1874;Lomell等人(1989)J,Clin,Chem,35:1826;Landegren等人(1988)Science 241:1077-1080;Van Brunt(1990)Biotechnology 8:291-294;Wu和Wallace(1989)Gene 4:560;Barringer等人(1990)Gene 89:117,以及Sooknanan和Malek(1995)Biotechnology 13:563-564。体外扩增核酸的改进方法被描述在Wallace等人的美国专利5,426,039中。通过PCR扩增大的核酸的改进方法总结在Cheng等人(1994)Nature 369:684-685及其引用的参考文献中,其中产生最高达40kb的PCR扩增子。熟练的技术人员懂得,基本上任何RNA可以被转化为适用于限制性消化、用逆转录酶和聚合酶进行的PCR扩增和测序的双链DNA,参见Ausbel,Sambrook和Berger,均同上文。
在一个方面中,本发明提供分离的或重组的多核苷酸,其选自由SEQ ID NO:516、517、518、519、520、521、522、523、524、525、526、527、528、529、530、531、532、533、534、535、536、537、538、539、540、541、542、543、544、545、546、547、548、549、550、551、552、553、554、555、556、557、558、559、560、561、562、563、564、565、566、567、620、622、624、626、628、630、632、634、636、638、640、642、644、646、648、650、652、654、656、658、660、662、664、666、668、670、672、674、676、678、680、682、684、686、688、690、692、694、696、698、700、702、704、706、708、710、712、714、716、718、720、722、724、726、728、730、732、734、736、738、740、742、744、746、748、750、752、754、756、758、760、762、764、768、770、772、774、776、778、780、782、784、786、788、790、792、794、796、798、800、802、804、806、808、810、812、814、816、818、820、822、824、832、834、836、838、840、842、844、846、848、850、852、854、856、858、860、862、864、866、868、870、872、874、876、878、880、882、884、886、888、890、892、894、896、898、900、902、904、906、908、910、912、914、916、918、920、922、924、926、928、930、932、933、934、935、936、937、938、939、940、941、942、943、944、945、947、949、951和952组成的组。
本发明的优选多核苷酸包括一种分离的或重组的多核苷酸序列,其编码一种氨基酸序列,采用B1OSUM62矩阵方法,存在一个缺口罚11分,延伸一个缺口罚1分,该氨基酸与选自由SEQ ID NO:300、445或457组成的组的氨基酸序列最佳地比对时,产生至少为460的相似性分值,其中至少如下位置之一遵从如下的限制:(i)在位置18和38上,存在一个Z5氨基酸残基;(ii)在位置62上,存在一个Z1氨基酸残基;(iii)在位置124上,存在一个Z6氨基酸残基;和(iv)在位置144上,存在一个Z2氨基酸残基,其中:Z1是选自由A、I、L、M和V组成的组的氨基酸残基;Z2是选自由F、W和Y组成的组的氨基酸残基;Z5是选自由D和E组成的组的氨基酸残基;和Z6是选自由C、G和P组成的组的氨基酸残基,和另外在氨基酸序列中对应于如下位置的氨基酸残基至少90%遵从如下限制:(a)在位置2、4、15、19、26、28、31、45、51、54、86、90、91、97、103、105、106、114、123、129、139和/或145上,氨基酸残基为B1;和(b)在位置3、5、8、10、11、14、17、24、27、32、37、47、48、49、52、57、58、61、63、68、69、79、80、82、83、89、92、100、101、104、119、120、125、126、128、131和/或143上,氨基酸残基为B2;其中B1是选自由A、I、L、M、F、W、Y和V组成的组的氨基酸;和B2是选自由R、N、D、C、Q、E、G、H、K、P、S和T组成的组的氨基酸。当用于指定一种氨基酸或者氨基酸残基残基时,单字母名称A、C、D、E、F、G、H、I、K、L、M、N、P、Q、R、S、T、V、W和Y具有本领域所用的标准含义,并在本文的表1中提供。
一些优选的本发明的分离的或重组的多核苷酸编码一种氨基酸序列,使得当采用B1OSUM62矩阵方法,存在一个缺口罚11分,延伸一个缺口罚1分,将该氨基酸序列与选自由SEQ ID NO:300、445或457组成的组的氨基酸序列最佳比对时,产生至少为460的相似性分值,如下位置的一个或多个遵从如下限制:(i)在位置18和38上,有Z5氨基酸残基;(ii)在位置62上,有Z1氨基酸残基;(iii)在位置124上,有Z6氨基酸残基;和(iv)在位置144上,有Z2氨基酸残基,其中:Z1是选自由A、I、L、M和V组成的组的氨基酸残基;Z2是选自由F、W和Y组成的组的氨基酸残基;Z5是选自由D和E组成的组的氨基酸残基;和Z6是选自由C、G和P组成的组的氨基酸残基,并且进一步地,氨基酸序列中对应于如下位置的氨基酸残基至少80%地遵从如下限制:(a)在位置2、4、15、19、26、28、31、45、51、54、86、90、91、97、103、105、106、114、123、129、139和/或145上,氨基酸残基为Z1;和(b)在位置31和/或45上,氨基酸残基为Z2;(c)在位置8上,氨基酸残基为Z3;(d)在位置89上,氨基酸残基为Z3或者Z6;(e)在位置82、92、101和/或120上,氨基酸残基为Z4;(f)在位置3、11、27和/或79上,氨基酸残基为Z5;(g)在位置18上,氨基酸残基为Z4或Z5;(h)在位置123上,氨基酸残基为Z1或Z2;(i)在位置12,33、35、39、53、59、112、132、135、140和/或146上,氨基酸残基为Z1或Z3;(j)在位置30上,氨基酸残基为Z1;(k)在位置6上,氨基酸残基为Z6;(l)在位置81上,氨基酸残基为Z2或Z4;(m)在位置113上,氨基酸残基为Z3;(n)在位置138上,氨基酸残基为Z4;(o)在位置142上,氨基酸残基为Z2;(p)在位置57和/或126上,氨基酸残基为Z3或Z4;(q)在位置5,17和61上,氨基酸残基为Z4;(r)在位置24上,氨基酸残基为Z3;(s)在位置104上,氨基酸残基为Z5;(t)在位置52和/或69上,氨基酸残基为Z3;(u)在位置14和/或119上,氨基酸残基为Z5;(v)在位置10、32、63和/或83上,氨基酸残基为Z5;(w)在位置48和/或80上,氨基酸残基为Z6;(x)在位置40上,氨基酸残基为Z1或Z2;(y)在位置96上,氨基酸残基为Z3或Z5;(z)在位置65上,氨基酸残基为Z3、Z4或Z6;(aa)在位置84和/或115上,氨基酸残基为Z3;(ab)在位置93上,氨基酸残基为Z4;(ac)在位置130上,氨基酸残基为Z2;(ad)在位置58上,氨基酸残基为Z3、Z4或者Z6;(ae)在位置47上,氨基酸残基为Z4或Z6;(af)在位置49和/或100上,氨基酸残基为Z3或Z4;(ag)在位置68上,氨基酸残基为Z4或Z5;(ah)在位置143上,氨基酸残基为Z4;(ai)在位置131上,氨基酸残基为Z5;(aj)在位置125和/或128上,氨基酸残基为Z5;(ak)在位置67上,氨基酸残基为Z3或Z4;(al)在位置60上,氨基酸残基为Z5;和(am)在位置37上,氨基酸残基为Z4或Z6;其中Z1是选自由A、I、L、M和V组成的组的氨基酸残基;Z2是选自由F、W和Y组成的组的氨基酸残基;Z3是选自由N、Q、S和T组成的组的氨基酸;Z4是选自由R、H和K组成的组的氨基酸;Z5是选自由D和E组成的组的氨基酸残基;和Z6是选自由C、G和P组成的组的氨基酸残基。
一些优选的本发明的分离的或重组的多核苷酸编码一种氨基酸序列,在氨基酸序列中对应于在(a)-(am)中指定的位置的氨基酸残基至少90%地遵从在(a)-(am)中指出的氨基酸残基的限制。
一些优选的本发明的分离的或重组的多核苷酸编码一种氨基酸序列,使得当将该氨基酸序列与选自由SEQ ID NO:300、445或457组成的组的氨基酸序列最佳比对时,氨基酸序列中的至少90%的氨基酸残基遵从如下限制:(a)在位置1、7、9、13、20、36、42、46、50、56、64、70、72、75、76、78、94、98、107、110、117、118、121和/或141上,氨基酸残基为B1;和(b)在位置16、21、22、23、25、29、34、41、43、44、55、66、71、73、74、77、85、87、88、95、99、102、108、109、111、116、122、127、133、134、136和/或137上,氨基酸残基为B2;其中B1是选自由A、I、L、M、F、W、Y和V组成的组的氨基酸;和B2是选自由R、N、D、C、Q、E、G、H、K、P、S和T组成的组的氨基酸。
一些优选的本发明的分离的或重组的多核苷酸编码一种氨基酸序列,使得当将该氨基酸序列与SEQ ID NO:300、445或457最佳比对时,氨基酸序列中至少90%的氨基酸残基遵从如下限制:(a)在位置1、7、9、13、20、42、46、50、56、64、70、72、75、76、78、94、98、107、110、117、118、121和/或141上,氨基酸残基为B1;和(b)在位置16、21、22、23、25、29、34、36、41、43、44、55、66、71、73、74、77、85、87、88、95、99、102、108、109、111、116、122、127、133、134、136和/或137上,氨基酸残基为B2;其中B1是选自由A、I、L、M、F、W、Y和V组成的组的氨基酸;和B2是选自由R、N、D、C、Q、E、G、H、K、P、S和T组成的组的氨基酸。
一些优选的本发明的分离的或重组的多核苷酸编码一种氨基酸序列,使得当将该氨基酸序列与SEQ ID NO:300、445或457最佳比对时,氨基酸序列中的至少90%氨基酸残基遵从如下限制:(a)在位置1、7、9、20、42、50、72、75、76、78、94、98、110、121和/或141上,氨基酸残基为Z1;(b)在位置13、46、56、70、107、117和/或118上,氨基酸残基为Z2;(c)在位置23、55、71、77、88和/或109上,氨基酸残基为Z3;(d)在位置16、21、41、73、85、99和/或111上,氨基酸残基为Z4;(e)在位置34和/或95上,氨基酸残基为Z5;(f)在位置22、25、29、43、44、66、74、87、102、108、116、122、127、133、134、136和/或137上,氨基酸残基为Z6;其中Z1是选自由A、I、L、M和V组成的组的氨基酸残基;Z2是选自由F、W和Y组成的组的氨基酸残基;Z3是选自由N、Q、S和T组成的组的氨基酸;Z4是选自由R、H和K组成的组的氨基酸;Z5是选自由D和E组成的组的氨基酸残基;和Z6是选自由C、G和P组成的组的氨基酸残基。
一些优选的本发明的分离的或重组的多核苷酸编码一种氨基酸序列,该氨基酸序列在位置36上包含选自由Z1和Z3组成的组的氨基酸残基。一些优选的本发明的分离的或重组的多核苷酸编码氨基酸序列,该氨基酸序列在位置64上包含选自由Z1和Z2组成的组的氨基酸残基。
一些优选的本发明的分离的或重组的多核苷酸编码一种氨基酸序列,使得当将该氨基酸序列与SEQ ID NO:300、445或457最佳比对时,氨基酸序列中的至少80%的氨基酸残基遵从如下限制:(a)在位置2上,氨基酸残基为I或L;(b)在位置3上,氨基酸残基为E;(c)在位置4上,氨基酸残基为V或I;(d)在位置5上,氨基酸残基为K;(e)在位置6上,氨基酸残基为P;(f)在位置8上,氨基酸残基为N;(g)在位置10上,氨基酸残基为E;(h)在位置11上,氨基酸残基为D或E;(i)在位置12上,氨基酸残基为T;(j)在位置14上,氨基酸残基为E或D;(k)在位置15上,氨基酸残基为L;(l)在位置17上,氨基酸残基为H;(m)在位置18上,氨基酸残基为R、E或K;(n)在位置19上,氨基酸残基为I或V;(o)在位置24上,氨基酸残基为Q;(p)在位置26上,氨基酸残基为M、L、V或I;(q)在位置27上,氨基酸残基为E;(r)在位置28上,氨基酸残基为A或V;(s)在位置30上,氨基酸残基为M;(t)在位置31上,氨基酸残基为Y或F;(u)在位置32上,氨基酸残基为E或D;(v)在位置33上,氨基酸残基为T或S;(w)在位置35上,氨基酸残基为L;(x)在位置37上,氨基酸残基为R、G、E或Q;(y)在位置39上,氨基酸残基为A或S;(z)在位置40上,氨基酸残基为F或L;(aa)在位置45上,氨基酸残基为Y或F;(ab)在位置47上,氨基酸残基为R或G;(ac)在位置48上,氨基酸残基为G;(ad)在位置49上,氨基酸残基为K、R或Q;(ae)在位置51上,氨基酸残基为I或V;(af)在位置52上,氨基酸残基为S;(ag)在位置53上,氨基酸残基为I或V;(ah)在位置54上,氨基酸残基为A;(ai)在位置57上,氨基酸残基为H或N;(aj)在位置58上,氨基酸残基为Q、K、R或P;(ak)在位置59上,氨基酸残基为A;(al)在位置60上,氨基酸残基为E;(am)在位置61上,氨基酸残基为H或R;(an)在位置63上,氨基酸残基为E或D;(ao)在位置65上,氨基酸残基为E、P或Q;(ap)在位置67上,氨基酸残基为Q或R;(aq)在位置68上,氨基酸残基为K或E;(ar)在位置69上,氨基酸残基为Q;(as)在位置79上,氨基酸残基为E;(at)在位置80上,氨基酸残基为G;(au)在位置81上,氨基酸残基为Y、H或F;(av)在位置82上,氨基酸残基为R;(aw)在位置83上,氨基酸残基为E或D;(ax)在位置84上,氨基酸残基为Q;(ay)在位置86上,氨基酸残基为A;(az)在位置89上,氨基酸残基为G、T或S;(ba)在位置90上,氨基酸残基为L;(bb)在位置91上,氨基酸残基为L、I或V;(bc)在位置92上,氨基酸残基为R或K;(bd)在位置93上,氨基酸残基为H;(be)在位置96上,氨基酸残基为E或Q;(bf)在位置97上,氨基酸残基为I;(bg)在位置100上,氨基酸残基为K或N;(bh)在位置101上,氨基酸残基为K或R;(bi)在位置103上,氨基酸残基为A或V;(bj)在位置104上,氨基酸残基为D;(bk)在位置105上,氨基酸残基为M、L或I;(bl)在位置106上,氨基酸残基为L;(bm)在位置112上,氨基酸残基为T或A;(bn)在位置113上,氨基酸残基为S或T;(bo)在位置114上,氨基酸残基为A;(bp)在位置115上,氨基酸残基为S;(bq)在位置119上,氨基酸残基为K或R;(br)在位置120上,氨基酸残基为K或R;(bs)在位置123上,氨基酸残基为F或L;(bt)在位置125上,氨基酸残基为E;(bu)在位置126上,氨基酸残基为Q或H;;(bv)在位置128上,氨基酸残基为E或D;(bw)在位置129上,氨基酸残基为V或I;(bx)在位置130上,氨基酸残基为F;(by)在位置131上,氨基酸残基为D或E;(bz)在位置132上,氨基酸残基为T;(ca)在位置135上,氨基酸残基为V;(cb)在位置138上,氨基酸残基为H;(cc)在位置139上,氨基酸残基为I;(cd)在位置140上,氨基酸残基为L或M;(ce)在位置142上,氨基酸残基为Y;(cf)在位置143上,氨基酸残基为K或R;(cg)在位置145上,氨基酸残基为L或I;和(ch)在位置146上,氨基酸残基为T。
一些优选的本发明的分离的或重组的多核苷酸编码一种氨基酸序列,使得当将该氨基酸序列与SEQ ID NO:300、445或457最佳比对时,氨基酸序列中的至少90%的氨基酸残基遵从在上面的(a)-(ch)中指出的氨基酸残基限制。
一些优选的本发明的分离的或重组的多核苷酸编码一种氨基酸序列,使得当采用B1OSUM62矩阵方法,存在一个缺口罚11分,延伸一个缺口罚1分,将该氨基酸序列与选自由SEQ ID NO:300、445或457组成的组的氨基酸序列最佳比对时,产生至少为460的相似性分值,如下位置的一个或多个遵从如下限制:(i)在位置18和38上,存在一个Z5氨基酸残基;(ii)在位置62上,存在一个Z1氨基酸残基;(iii)在位置124上上,存在一个Z6氨基酸残基;和(iv)在位置144上,存在一个Z2氨基酸残基;其中:Z1是选自由A、I、L、M和V组成的组的氨基酸残基;Z2是选自由F、W和Y组成的组的氨基酸残基;Z5是选自由D和E组成的组的氨基酸残基;和Z6是选自由C、G和P组成的组的氨基酸残基,并且另外地,氨基酸序列中对应于如下位置的氨基酸残基至少80%地遵从如下限制:(a)在位置9、76、94和110上,氨基酸残基为A;(b)在位置29和108上,氨基酸残基为C;(c)在位置34上,氨基酸残基为D;(d)在位置95上,氨基酸残基为E;(e)在位置56上,氨基酸残基为F;(f)在位置43、44、66、74、87、102、116、122、127和136上,氨基酸残基为G;(g)在位置41上,氨基酸残基为H;(h)在位置7上,氨基酸残基为I;(i)在位置85上,氨基酸残基为K;(j)在位置20、42、50、78和121上,氨基酸残基为L;(k)在位置1和141上,氨基酸残基为M;(l)在位置23和109上,氨基酸残基为N;(m)在位置22、25、133、134和137上,氨基酸残基为P;(n)在位置71上,氨基酸残基为Q;(o)在位置16、21、73、99和111上,氨基酸残基为R;(p)在位置55上,氨基酸残基为S;(q)在位置77上,氨基酸残基为T;(r)在位置107上,氨基酸残基为W;和(s)在位置13、46、70和118上,氨基酸残基为Y。
一些优选的本发明的分离的或重组的多核苷酸编码一种氨基酸序列,其遵从至少如下额外限制之一:(a)在位置36上,氨基酸残基为M、L或T;(b)在位置72上,氨基酸残基为L或I;(c)在位置75上,氨基酸残基为M或V;(d)在位置64上,氨基酸残基为L,I或F;(e)在位置88上,氨基酸残基为T或S;(f)在位置117上,氨基酸残基为Y或F。
一些优选的本发明的分离的或重组的多核苷酸编码一种氨基酸序列,其遵从至少如下额外限制之一:(a)在位置14上,氨基酸残基为D;(b)在位置18上,氨基酸残基为E;(c)在位置26上,氨基酸残基为M或V;(e)在位置30上,氨基酸残基为I;(f)在位置32上,氨基酸残基为D;(g)在位置36上,氨基酸残基为M或T;(i)在位置37上,氨基酸残基为C;(j)在位置38上,氨基酸残基为D;(j)在位置53上,氨基酸残基为V;(k)在位置58上,氨基酸残基为R;(l)在位置61上,氨基酸残基为R;(m)在位置62上,氨基酸残基为L;(n)在位置64上,氨基酸残基为I或F;(o)在位置65上,氨基酸残基为P;(p)在位置72上,氨基酸残基为I;(q)在位置75上,氨基酸残基为V;(r)在位置88上,氨基酸残基为T;(s)在位置89上,氨基酸残基为G;(t)在位置91上,氨基酸残基为L;(u)在位置98上,氨基酸残基为I;(v)在位置105上,氨基酸残基为I;(w)在位置112上,氨基酸残基为A;(x)在位置124上,氨基酸残基为G或C;(y)在位置128上,氨基酸残基为D;(z)在位置140上,氨基酸残基为M;(aa)在位置143上,氨基酸残基为R;和(ab)在位置144上,氨基酸残基为W。
一些优选的本发明的分离的或重组的多核苷酸编码一种氨基酸序列,其中氨基酸序列中对应于在上述(a)-(ab)中指定的位置的氨基酸残基至少80%地遵从在(a)-(ab)中指定的氨基酸残基的限制。
一些优选的本发明的分离的或重组的多核苷酸编码一种氨基酸序列,其遵从至少如下的额外限制之一:(a)在位置41上,氨基酸残基为H;(b)在位置138上,氨基酸残基为H;(c)在位置34上,氨基酸残基为N;和(d)在位置55上,氨基酸残基为S。
一些优选的本发明的分离的或重组的多核苷酸选自由如下组成的组:(a)编码与SEQ ID NO:577至少98%相同的氨基酸序列的核苷酸序列;(b)编码与SEQ ID NO:578至少97%相同的氨基酸序列的核苷酸序列;(c)编码与SEQ ID NO:621至少97%相同的氨基酸序列的核苷酸序列;(d)编码与SEQ ID NO:579至少98%相同的氨基酸序列的核苷酸序列;(e)编码与SEQ ID NO:602至少98%相同的氨基酸序列的核苷酸序列;(f)编码与SEQ ID NO:697至少95%相同的氨基酸序列的核苷酸序列;(g)编码与SEQ ID NO:712至少96%相同的氨基酸序列的核苷酸序列;(h)编码与SEQ ID NO:613至少97%相同的氨基酸序列的核苷酸序列;(i)编码与SEQ ID NO:677至少89%相同的氨基酸序列的核苷酸序列;(j)编码与SEQ ID NO:584至少95%相同的氨基酸序列的核苷酸序列;(k)编码与SEQ ID NO:707至少98%相同的氨基酸序列的核苷酸序列;(l)编码与SEQ ID NO:616至少98%相同的氨基酸序列的核苷酸序列;(m)编码与SEQ ID NO:612至少96%相同的氨基酸序列的核苷酸序列;和(n)编码与SEQ IDNO:590至少98%相同的氨基酸序列的核苷酸序列。
一些优选的本发明的分离的或重组的多核苷酸选自由如下组成的组:(a)编码与SEQ ID NO:577至少98%相同的氨基酸序列的核苷酸序列;(b)编码与SEQ ID NO:578至少97%相同的氨基酸序列的核苷酸序列;(c)编码与SEQ ID NO:621至少97%相同的氨基酸序列的核苷酸序列;(d)编码与SEQ ID NO:579至少98%相同的氨基酸序列的核苷酸序列;(e)编码与SEQ ID NO:602至少98%相同的氨基酸序列的核苷酸序列;(f)编码与SEQ ID NO:697至少95%相同的氨基酸序列的核苷酸序列;(g)编码与SEQ ID NO:712至少96%相同的氨基酸序列的核苷酸序列;(h)编码与SEQ ID NO:613至少97%相同的氨基酸序列的核苷酸序列;(i)编码与SEQ ID NO:677至少89%相同的氨基酸序列的核苷酸序列;(j)编码与SEQ ID NO:584至少95%相同的氨基酸序列的核苷酸序列;(k)编码与SEQ ID NO:707至少98%相同的氨基酸序列的核苷酸序列;(l)编码与SEQ ID NO:616至少98%相同的氨基酸序列的核苷酸序列;(m)编码与SEQ ID NO:612至少96%相同的氨基酸序列的核苷酸序列;和(n)编码与SEQ IDNO:590至少98%相同的氨基酸序列的核苷酸序列,其中如下位置遵从如下限制:(i)在位置18和38上,存在一个Z5氨基酸残基;(ii)在位置62上,存在一个Z1氨基酸残基;(iii)在位置124上,存在一个Z6氨基酸残基;和(iv)在位置144上,存在一个Z2氨基酸残基,其中:其中Z1是选自由A、I、L、M和V组成的组的氨基酸残基;Z2是选自由F、W和Y组成的组的氨基酸残基;Z5是选自由D和E组成的组的氨基酸残基;和Z6是选自由C、G和P组成的组的氨基酸残基。
一些优选的本发明的分离的或重组的多核苷酸选自由如下组成的组:(a)编码与SEQ ID NO:577至少98%相同的氨基酸序列的核苷酸序列;(b)编码与SEQ ID NO:578至少97%相同的氨基酸序列的核苷酸序列;(c)编码与SEQ ID NO:621至少97%相同的氨基酸序列的核苷酸序列;(d)编码与SEQ ID NO:579至少98%相同的氨基酸序列的核苷酸序列;(e)编码与SEQ ID NO:602至少98%相同的氨基酸序列的核苷酸序列;(f)编码与SEQ ID NO:697至少95%相同的氨基酸序列的核苷酸序列;(g)编码与SEQ ID NO:712至少96%相同的氨基酸序列的核苷酸序列;(h)编码与SEQ ID NO:613至少97%相同的氨基酸序列的核苷酸序列;(i)编码与SEQ ID NO:677至少89%相同的氨基酸序列的核苷酸序列;(j)编码与SEQ ID NO:584至少95%相同的氨基酸序列的核苷酸序列;(k)编码与SEQ ID NO:707至少98%相同的氨基酸序列的核苷酸序列;(l)编码与SEQ ID NO:616至少98%相同的氨基酸序列的核苷酸序列;(m)编码与SEQ ID NO:612至少96%相同的氨基酸序列的核苷酸序列;和(n)编码与SEQ IDNO:590至少98%相同的氨基酸序列的核苷酸序列,另外氨基酸序列中对应于如下位置的氨基酸残基至少90%遵从如下限制:(a)在位置2、4、15、19、26、28、31、45、51、54、86、90、91、97、103、105、106、114、123、129、139和/或145上,氨基酸残基为B1;和(b)在位置3、5、8、10、11、14、17、24、27、32、37、47、48、49、52、57、58、61、63、68、69、79、80、82、83、89、92、100、101、104、119、120、125、126、128、131和/或143上,氨基酸残基为B2;其中B1是选自由A、I、L、M、F、W、Y和V组成的组的氨基酸;和B2是选自由R、N、D、C、Q、E、G、H、K、P、S和T组成的组的氨基酸。
一些优选的本发明的分离的或重组的多核苷酸选自由如下组成的组:(a)编码与SEQ ID NO:577至少98%相同的氨基酸序列的核苷酸序列;(b)编码与SEQ ID NO:578至少97%相同的氨基酸序列的核苷酸序列;(c)编码与SEQ ID NO:621至少97%相同的氨基酸序列的核苷酸序列;(d)编码与SEQ ID NO:579至少98%相同的氨基酸序列的核苷酸序列;(e)编码与SEQ ID NO:602至少98%相同的氨基酸序列的核苷酸序列;(f)编码与SEQ ID NO:697至少95%相同的氨基酸序列的核苷酸序列;(g)编码与SEQ ID NO:712至少96%相同的氨基酸序列的核苷酸序列;(h)编码与SEQ ID NO:613至少97%相同的氨基酸序列的核苷酸序列;(i)编码与SEQ ID NO:677至少89%相同的氨基酸序列的核苷酸序列;(j)编码与SEQ ID NO:584至少95%相同的氨基酸序列的核苷酸序列;(k)编码与SEQ ID NO:707至少98%相同的氨基酸序列的核苷酸序列;(l)编码与SEQ ID NO:616至少98%相同的氨基酸序列的核苷酸序列;(m)编码与SEQ ID NO:612至少96%相同的氨基酸序列的核苷酸序列;和(n)编码与SEQ IDNO:590至少98%相同的氨基酸序列的核苷酸序列,另外氨基酸序列中对应于如下位置的氨基酸残基至少80%地遵从如下限制:(a)在位置2、4、15、19、26、28、31、45、51、54、86、90、91、97、103、105、106、114、123、129、139和/或145上,氨基酸残基为Z1;和(b)在位置31和/或45上,氨基酸残基为Z2;(c)在位置8上,氨基酸残基为Z3;(d)在位置89上,氨基酸残基为Z3或者Z6;(e)在位置82、92、101和/或120上,氨基酸残基为Z4;(f)在位置3、11、27和/或79上,氨基酸残基为Z5;(g)在位置18上,氨基酸残基为Z4或Z5;(h)在位置123上,氨基酸残基为Z1或Z2;(i)在位置12、33、35、39、53、59、112、132、135、140和/或146上,氨基酸残基为Z1或Z3;(j)在位置30上,氨基酸残基为Z1;(k)在位置6上,氨基酸残基为Z6;(l)在位置81上,氨基酸残基为Z2或Z4;(m)在位置113上,氨基酸残基为Z3;(n)在位置138上,氨基酸残基为Z4;(o)在位置142上,氨基酸残基为Z2;(p)在位置57和/或126上,氨基酸残基为Z3或Z4;(q)在位置5,17和61上,氨基酸残基为Z4;(r)在位置24上,氨基酸残基为Z3;(s)在位置104上,氨基酸残基为Z5;(t)在位置52和/或69上,氨基酸残基为Z3;(u)在位置14和/或119上,氨基酸残基为Z5;(v)在位置10、32、63和/或83上,氨基酸残基为Z5;(w)在位置48和/或80上,氨基酸残基为Z6;(x)在位置40上,氨基酸残基为Z1或Z2;(y)在位置96上,氨基酸残基为Z3或Z5;(z)在位置65上,氨基酸残基为Z3、Z4或Z6;(aa)在位置84和/或115上,氨基酸残基为Z3;(ab)在位置93上,氨基酸残基为Z4;(ac)在位置130上,氨基酸残基为Z2;(ad)在位置58上,氨基酸残基为Z3、Z4或者Z6;(ae)在位置47上,氨基酸残基为Z4或Z6;(af)在位置49和/或100上,氨基酸残基为Z3或Z4;(ag)在位置68上,氨基酸残基为Z4或Z5;(ah)在位置143上,氨基酸残基为Z4;(ai)在位置131上,氨基酸残基为Z5;(aj)在位置125和/或128上,氨基酸残基为Z5;(ak)在位置67上,氨基酸残基为Z3或Z4;(al)在位置60上,氨基酸残基为Z5;和(am)在位置37上,氨基酸残基为Z4或Z6;其中Z1是选自由A、I、L、M和V组成的组的氨基酸残基;Z2是选自由F、W和Y组成的组的氨基酸残基;Z3是选自由N、Q、S和T组成的组的氨基酸;Z4是选自由R、H和K组成的组的氨基酸;Z5是选自由D和E组成的组的氨基酸残基;和Z6是选自由C、G和P组成的组的氨基酸残基。
一些优选的本发明的分离的或重组的多核苷酸编码一种氨基酸序列,另外其中氨基酸序列中对应于在(a)-(am)中指定的位置的氨基酸残基至少90%地遵从在(a)-(am)中指定的氨基酸残基的限制。
一些优选的本发明的分离的或重组的多核苷酸编码一种氨基酸序列,其中氨基酸序列中对应于如下位置的氨基酸残基至少90%地遵从如下的额外限制:(a)在位置1、7、9、13、20、36、42、46、50、56、64、70、72、75、76、78、94、98、107、110、117、118、121和/或141上,氨基酸残基为B1;和(b)在位置16、21、22、23、25、29、34、41、43、44、55、66、71、73、74、77、85、87、88、95、99、102、108、109、111、116、122、127、133、134、136和/或137上,氨基酸残基为B2;其中B1是选自由A、I、L、M、F、W、Y和V组成的组的氨基酸;和B2是选自由R、N、D、C、Q、E、G、H、K、P、S和T组成的组的氨基酸。
一些优选的本发明的分离的或重组的多核苷酸编码一种氨基酸序列,使得当该氨基酸序列与SEQ ID NO:300、445或457最佳地比对时,氨基酸序列中的至少90%的氨基酸残基遵从如下限制:(a)在位置1、7、9、13、20、42、46、50、56、64、70、72、75、76、78、94、98、107、110、117、118、121和/或141上,氨基酸残基为B1;和(b)在位置16、21、22、23、25、29、34、36、41、43、44、55、66、71、73、74、77、85、87、88、95、99、102、108、109、111、116、122、127、133、134、136和/或137上,氨基酸残基为B2;其中B1是选自由A、I、L、M、F、W、Y和V组成的组的氨基酸;和B2是选自由R、N、D、C、Q、E、G、H、K、P、S和T组成的组的氨基酸。
一些优选的本发明的分离的或重组的多核苷酸编码一种氨基酸序列,使得当该氨基酸序列与SEQ ID NO:300、445或457最佳地比对时,氨基酸序列中的至少90%的氨基酸残基遵从如下限制:(a)在位置1、7、9、20、42、50、72、75、76、78、94、98、110、121和/或141上,氨基酸残基为Z1;(b)在位置13、46、56、70、107、117和/或118上,氨基酸残基为Z2;(c)在位置23、55、71、77、88和/或109上,氨基酸残基为Z3;(d)在位置16、21、41、73、85、99和/或111上,氨基酸残基为Z4;(e)在位置34和/或95上,氨基酸残基为Z5;(f)在位置22、25、29、43、44、66、74、87、102、108、116、122、127、133、134、136和/或137上,氨基酸残基为Z6;其中Z1是选自由A、I、L、M和V组成的组的氨基酸残基;Z2是选自由F、W和Y组成的组的氨基酸残基;Z3是选自由N、Q、S和T组成的组的氨基酸;Z4是选自由R、H和K组成的组的氨基酸;Z5是选自由D和E组成的组的氨基酸残基;和Z6是选自由C、G和P组成的组的氨基酸残基。
一些优选的本发明的分离的或重组的多核苷酸编码一种氨基酸序列,该氨基酸序列在位置36上包含选自由Z1和Z3组成的组的氨基酸残基。一些优选的本发明的分离的或重组的多核苷酸编码氨基酸序列,该氨基酸序列在位置64上包含选自由Z1和Z2组成的组的氨基酸残基。
一些优选的本发明的分离的或重组的多核苷酸编码一种氨基酸序列,使得当该氨基酸序列与SEQ ID NO:300、445或457最佳地比对时,氨基酸序列中至少80%的氨基酸残基遵从如下限制:(a)在位置2上,氨基酸残基为I或L;(b)在位置3上,氨基酸残基为E;(c)在位置4上,氨基酸残基为V或I;(d)在位置5上,氨基酸残基为K;(e)在位置6上,氨基酸残基为P;(f)在位置8上,氨基酸残基为N;(g)在位置10上,氨基酸残基为E;(h)在位置11上,氨基酸残基为D或E;(i)在位置12上,氨基酸残基为T;(j)在位置14上,氨基酸残基为E或D;(k)在位置15上,氨基酸残基为L;(l)在位置17上,氨基酸残基为H;(m)在位置18上,氨基酸残基为R、E或K;(n)在位置19上,氨基酸残基为I或V;(o)在位置24上,氨基酸残基为Q;(p)在位置26上,氨基酸残基为M、L、V或I;(q)在位置27上,氨基酸残基为E;(r)在位置28上,氨基酸残基为A或V;(s)在位置30上,氨基酸残基为M;(t)在位置31上,氨基酸残基为Y或F;(u)在位置32上,氨基酸残基为E或D;(v)在位置33上,氨基酸残基为T或S;(w)在位置35上,氨基酸残基为L;(x)在位置37上,氨基酸残基为R、G、E或Q;(y)在位置39上,氨基酸残基为A或S;(z)在位置40上,氨基酸残基为F或L;(aa)在位置45上,氨基酸残基为Y或F;(ab)在位置47上,氨基酸残基为R或G;(ac)在位置48上,氨基酸残基为G;(ad)在位置49上,氨基酸残基为K、R或Q;(ae)在位置51上,氨基酸残基为I或V;(af)在位置52上,氨基酸残基为S;(ag)在位置53上,氨基酸残基为I或V;(ah)在位置54上,氨基酸残基为A;(ai)在位置57上,氨基酸残基为H或N;(aj)在位置58上,氨基酸残基为Q、K、R或P;(ak)在位置59上,氨基酸残基为A;(al)在位置60上,氨基酸残基为E;(am)在位置61上,氨基酸残基为H或R;(an)在位置63上,氨基酸残基为E或D;(ao)在位置65上,氨基酸残基为E、P或Q;(ap)在位置67上,氨基酸残基为Q或R;(aq)在位置68上,氨基酸残基为K或E;(ar)在位置69上,氨基酸残基为Q;(as)在位置79上,氨基酸残基为E;(at)在位置80上,氨基酸残基为G;(au)在位置81上,氨基酸残基为Y、H或F;(av)在位置82上,氨基酸残基为R;(aw)在位置83上,氨基酸残基为E或D;(ax)在位置84上,氨基酸残基为Q;(ay)在位置86上,氨基酸残基为A;(az)在位置89上,氨基酸残基为G、T或S;(ba)在位置90上,氨基酸残基为L;(bb)在位置91上,氨基酸残基为L、I或V;(bc)在位置92上,氨基酸残基为R或K;(bd)在位置93上,氨基酸残基为H;(be)在位置96上,氨基酸残基为E或Q;(bf)在位置97上,氨基酸残基为I;(bg)在位置100上,氨基酸残基为K或N;(bh)在位置101上,氨基酸残基为K或R;(bi)在位置103上,氨基酸残基为A或V;(bj)在位置104上,氨基酸残基为D;(bk)在位置105上,氨基酸残基为M、L或I;(Bl)在位置106上,氨基酸残基为L;(bm)在位置112上,氨基酸残基为T或A;(bn)在位置113上,氨基酸残基为S或T;(bo)在位置114上,氨基酸残基为A;(bp)在位置115上,氨基酸残基为S;(bq)在位置119上,氨基酸残基为K或R;(br)在位置120上,氨基酸残基为K或R;(bs)在位置123上,氨基酸残基为F或L;(bt)在位置125上,氨基酸残基为E;(bu)在位置126上,氨基酸残基为Q或H;;(bv)在位置128上,氨基酸残基为E或D;(bw)在位置129上,氨基酸残基为V或I;(bx)在位置130上,氨基酸残基为F;(by)在位置131上,氨基酸残基为D或E;(bx)在位置132上,氨基酸残基为T;(ca)在位置135上,氨基酸残基为V;(cb)在位置138上,氨基酸残基为H;(cc)在位置139上,氨基酸残基为I;(cd)在位置140上,氨基酸残基为L或M;(ce)在位置142上,氨基酸残基为Y;(cf)在位置143上,氨基酸残基为K或R;(cg)在位置145上,氨基酸残基为L或I;和(ch)在位置146上,氨基酸残基为T。
一些优选的本发明的分离的或重组的多核苷酸编码一种氨基酸序列,使得当将该氨基酸序列与SEQ ID NO:300、445或457最佳比对时,氨基酸序列中的至少90%氨基酸残基遵从在上面的(a)-(ch)中指出的氨基酸残基限制。
一些优选的本发明的分离的或重组的多核苷酸选自由如下组成的组:(a)编码与SEQ ID NO:577至少98%相同的氨基酸序列的核苷酸序列;(b)编码与SEQ ID NO:578至少97%相同的氨基酸序列的核苷酸序列;(c)编码与SEQ ID NO:621至少97%相同的氨基酸序列的核苷酸序列;(d)编码与SEQ ID NO:579至少98%相同的氨基酸序列的核苷酸序列;(e)编码与SEQ ID NO:602至少98%相同的氨基酸序列的核苷酸序列;(f)编码与SEQ ID NO:697至少95%相同的氨基酸序列的核苷酸序列;(g)编码与SEQ ID NO:712至少96%相同的氨基酸序列的核苷酸序列;(h)编码与SEQ ID NO:613至少97%相同的氨基酸序列的核苷酸序列;(i)编码与SEQ ID NO:677至少89%相同的氨基酸序列的核苷酸序列;(j)编码与SEQ ID NO:584至少95%相同的氨基酸序列的核苷酸序列;(k)编码与SEQ ID NO:707至少98%相同的氨基酸序列的核苷酸序列;(l)编码与SEQ ID NO:616至少98%相同的氨基酸序列的核苷酸序列;(m)编码与SEQ ID NO:612至少96%相同的氨基酸序列的核苷酸序列;和(n)编码与SEQ IDNO:590至少98%相同的氨基酸序列的核苷酸序列,氨基酸序列中对应于如下位置的氨基酸残基至少80%的遵从如下限制:(a)在位置9、76、94和110上,氨基酸残基为A;(b)在位置29和108上,氨基酸残基为C;(c)在位置34上,氨基酸残基为D;(d)在位置95上,氨基酸残基为E;(e)在位置56上,氨基酸残基为F;(f)在位置43、44、66、74、87、102、116、122、127和136上,氨基酸残基为G;(g)在位置41上,氨基酸残基为H;(h)在位置7上,氨基酸残基为I;(i)在位置85上,氨基酸残基为K;(j)在位置20、42、50、78和121上,氨基酸残基为L;(k)在位置1和141上,氨基酸残基为M;(l)在位置23和109上,氨基酸残基为N;(m)在位置22、25、133、134和137上,氨基酸残基为P;(n)在位置71上,氨基酸残基为Q;(o)在位置16、21、73、99和111上,氨基酸残基为R;(p)在位置55上,氨基酸残基为S;(q)在位置77上,氨基酸残基为T;(r)在位置107上,氨基酸残基为W;和(s)在位置13、46、70和118上,氨基酸残基为Y。
一些优选的本发明的分离的或重组的多核苷酸编码一种氨基酸序列,该氨基酸序列还包含至少一个满足如下标准的氨基酸残基:(a)在位置14上,氨基酸残基为D;(b)在位置18上,氨基酸残基为E;(c)在位置26上,氨基酸残基为M或V;(e)在位置30上,氨基酸残基为I;(f)在位置32上,氨基酸残基为D;(g)在位置36上,氨基酸残基为M或T;(i)在位置37上,氨基酸残基为C;(j)在位置38上,氨基酸残基为D;(j)在位置53上,氨基酸残基为V;(k)在位置58上,氨基酸残基为R;(l)在位置61上,氨基酸残基为R;(m)在位置62上,氨基酸残基为L;(n)在位置64上,氨基酸残基为I或F;(o)在位置65上,氨基酸残基为P;(p)在位置72上,氨基酸残基为I;(q)在位置75上,氨基酸残基为V;(r)在位置88上,氨基酸残基为T;(s)在位置89上,氨基酸残基为G;(t)在位置91上,氨基酸残基为L;(u)在位置98上,氨基酸残基为I;(v)在位置105上,氨基酸残基为I;(w)在位置112上,氨基酸残基为A;(x)在位置124上,氨基酸残基为G或C;(y)在位置128上,氨基酸残基为D;(z)在位置140上,氨基酸残基为M;(aa)在位置143上,氨基酸残基为R;和(ab)在位置144上,氨基酸残基为W。
一些优选的本发明的分离的或重组的多核苷酸编码一种氨基酸序列,使得当将该氨基酸序列与SEQ ID NO:300、445或457最佳比对时,氨基酸序列中的至少80%氨基酸残基遵从在上面的(a)-(ab)中指出的氨基酸残基限制。
一些优选的本发明的分离的或重组的多核苷酸,其包含编码选自由如下组成的组的氨基酸序列的核苷酸序列:(a)与SEQ ID NO:919至少96%相同的氨基酸序列(例如SEQ ID NO:917、919、921、923、925、927、833、835、839、843、845、859、863、873、877、891、895、901、905、907、913、915或950);(b)与SEQ ID NO:929至少97%相同的氨基酸序列(例如,编码SEQ ID NO:929、931、835、843、849或867的核苷酸序列);(c)与SEQ ID NO:847至少98%相同的氨基酸序列(例如编码SEQ ID NO:845或847的核苷酸序列);(d)与SEQ ID NO:851至少98%的氨基酸序列;(e)与SEQ ID NO:853至少98%相同的氨基酸序列;(f)与SEQ ID NO:855至少98%相同的氨基酸序列(例如编码SEQ ID NO:835或855的核苷酸序列);(g)与SEQ ID NO:857至少98%相同的氨基酸序列;(h)与SEQ IDNO:861至少98%相同的氨基酸序列(例如编码SEQ ID NO:839、861或883的核苷酸序列);(i)与SEQ ID NO:871至少98%相同的氨基酸序列;(j)与SEQ ID NO:875至少98%相同的氨基酸序列;(k)与SEQ ID NO:881至少98%相同的氨基酸序列;(l)与SEQ IDNO:885至少98%相同的氨基酸序列(例如编码SEQ ID NO:845或885的核苷酸序列);(m)与SEQ ID NO:887至少98%相同的氨基酸序列;(n)与SEQ ID NO:889至少98%相同的氨基酸序列(例如,编码SEQ ID NO:863、889、891或903的核苷酸序列);(o)与SEQID NO:893至少98%相同的氨基酸序列;(p)与SEQ ID NO:897至少98%相同的氨基酸序列;(q)与SEQ ID NO:899至少98%相同的氨基酸序列;(r)与SEQ ID NO:909至少98%相同的氨基酸序列(例如,编码SEQ ID NO:883或909的核苷酸序列);(s)与SEQ IDNO:911至少98%相同的氨基酸序列;(t)与SEQ ID NO:837至少99%相同的氨基酸序列;(u)与SEQ ID NO:841至少99%相同的氨基酸序列;(v)与SEQ ID NO:865至少99%相同的氨基酸序列;(w)与SEQ ID NO:869至少99%相同的氨基酸序列;和(x)与SEQ ID NO:879至少99%相同的氨基酸序列。
一些优选的本发明的分离的或重组的多核苷酸选自由如下组成的组:(a)编码与SEQ ID NO:919至少96%相同的氨基酸序列的核苷酸序列(例如一种核苷酸序列,如SEQ ID NO:916、918、920、922、924、926、832、834、838、842、844、858、862、872、876、890、894、900、904、906、912、914、939、940、941、942、943、944、949、951或952);(b)编码与SEQ ID NO:929至少97%相同的氨基酸序列的核苷酸序列(例如一种核苷酸序列,如SEQ ID NO:928、930、834、842、848、866、936或937);(c)编码与SEQ ID NO:847至少98%相同的氨基酸序列的核苷酸序列(例如一种核苷酸序列,如SEQ ID NO:844或846);(d)编码与SEQ ID NO:851至少98%相同的氨基酸序列的核苷酸序列(例如一种核苷酸序列,如SEQ IDNO:850);(e)编码与SEQ ID NO:853至少98%相同的氨基酸序列的核苷酸序列(例如一种核苷酸序列,SEQ ID NO:852);(f)编码与SEQ ID NO:855至少98%相同的氨基酸序列的核苷酸序列(例如一种核苷酸序列,如SEQ ID NO:834或854);(g)编码与SEQ IDNO;857至少98%相同的氨基酸序列的核苷酸序列(例如一种核苷酸序列,如SEQ ID NO:856);(h)编码与SEQ ID NO:861至少98%相同的氨基酸序列的核苷酸序列(例如一种核苷酸序列,如SEQ IDNO:838、860或882);(i)编码与SEQ ID NO:871至少98%相同的氨基酸序列的核苷酸序列(例如一种核苷酸序列,如SEQ ID NO:870);(j)编码与SEQ ID NO:875至少98%相同的氨基酸序列的核苷酸序列(例如一种核苷酸序列,如SEQ ID NO:874);(k)编码与SEQ ID NO:881至少98%相同的氨基酸序列的核苷酸序列(例如一种核苷酸序列,如SEQ ID NO:880);(l)编码与SEQ ID NO:885至少98%相同的氨基酸序列的核苷酸序列(例如一种核苷酸序列,如SEQ ID NO:844或884);(m)编码与SEQ ID NO:887至少98%相同的氨基酸序列的核苷酸序列(例如一种核苷酸序列,如SEQ IDNO:886);(n)编码与SEQ ID NO:889至少98%相同的氨基酸序列的核苷酸序列(例如一种核苷酸序列,如SEQ ID NO:862,888,890或902);(o)编码与SEQ ID NO:893至少98%相同的氨基酸序列的核苷酸序列(例如一种核苷酸序列,如SEQ ID NO:892);(p)编码与SEQ ID NO:897至少98%相同的氨基酸序列的核苷酸序列(例如一种核苷酸序列,如SEQ ID NO:896);(q)编码与SEQ ID NO:899至少98%相同的氨基酸序列的核苷酸序列(例如一种核苷酸序列,如SEQ ID NO:898);(r)编码与SEQ ID NO:909至少98%相同的氨基酸序列的核苷酸序列(例如一种核苷酸序列,如SEQ ID NO:882或908);(s)编码与SEQ ID NO:911至少98%相同的氨基酸序列的核苷酸序列(例如一种核苷酸序列,如SEQ ID NO:910);(t)编码与SEQ ID NO:837至少99%相同的氨基酸序列的核苷酸序列(例如一种核苷酸序列,如SEQ ID NO:836);(u)编码与SEQ ID NO:841至少99%相同的氨基酸序列的核苷酸序列(例如一种核苷酸序列,如SEQ ID NO:840);(v)编码与SEQ ID NO:865至少99%相同的氨基酸序列的核苷酸序列(例如一种核苷酸序列,如SEQ ID NO:864);(w)编码与SEQ ID NO:869至少99%相同的氨基酸序列的核苷酸序列(例如一种核苷酸序列,如SEQ ID NO:868);和(x)编码与SEQ ID NO:879至少99%相同的氨基酸序列的核苷酸序列(例如一种核苷酸序列,如SEQ ID NO:878)。
一些优选的本发明的分离的或重组的多核苷酸包含编码氨基酸序列的核苷酸序列,该氨基酸序列与SEQ ID NO:929相比至少95%相同,并且在对应于SEQ ID NO:929的位置33的氨基酸位置上包含Gly或Asn残基(例如,编码SEQ ID NO:837、849、893、897、905、921、927、929或931的核苷酸序列)。一些优选的本发明的分离的或重组的多核苷酸包含编码氨基酸序列的核苷酸序列,该氨基酸序列与SEQID NO:929相比至少95%相同,并且在对应于SEQ ID NO:929的位置33的氨基酸位置上包含Gly或Asn残基(例如核苷酸序列如SEQ IDNO:836、848、892、896、904、920、926、928、930、938)。
一些优选的本发明的分离的或重组的多核苷酸编码一种氨基酸序列,该氨基酸序列还包括一个或多个满足如下标准的氨基酸残基:(a)在位置41上,氨基酸残基为H;(b)在位置138上,氨基酸残基为H;(c)在位置34上,氨基酸残基为N;和(d)在位置55上,氨基酸残基为S。
虽然对本发明的多肽的描述在此有时被表示为一列在特定位置上存在何种氨基酸残基的可能限制,在有些实施方案中,本发明的多肽满足一组特定的可能限制的全部条件。也就是说,在本文的有些情形下,一列可能限制表示为一列通过连接词“和/或”连接的选项,在有些实施方案中,各个这种连接作为“和”而不是“或”。在有些实施方案中,表示为可替代的可能性的可能限制都存在于本发明的多肽中;这仅当可替代的可能性不相互排斥时,才是正确的。
序列变体
本领域技术人员将会认识到,由于遗传密码的简并性,多种编码本发明的GAT多肽的核苷酸序列都可以被生产,其中一些和本文明确公开的核苷酸序列具有实质上的同一性。
表1
密码子表
氨基酸 | 密码子 | ||
丙氨酸 | Ala | A | GCA GCC GCG GCU |
半胱氨酸 | Cys | C | UGC UGU |
天冬氨酸 | Asp | D | GAC GAU |
谷氨酸 | Glu | E | GAA GAG |
苯丙氨酸 | Phe | F | UUC UUU |
甘氨酸 | Gly | G | GGA GGC GGG GGU |
组氨酸 | His | H | CAC CAU |
异亮氨酸 | Ile | I | AUA AUC AUU |
赖氨酸 | Lys | K | AAA AAG |
亮氨酸 | Leu | L | UUA UUG CUA CUC CUG CUU |
甲硫氨酸 | Met | M | AUG |
天冬酰胺 | Asn | N | AAC AAU |
脯氨酸 | Pro | P | CCA CCC CCG CCU |
谷氨酰胺 | Gln | Q | CAA CAG |
精氨酸 | Arg | R | AGA AGG CGA CGC CGG CGU |
丝氨酸 | Ser | S | AGC AGU UCA UCC UCG UCU |
苏氨酸 | Thr | T | ACA ACC ACG ACU |
缬氨酸 | Val | V | GUA GUC GUG GUU |
色氨酸 | Trp | W | UGG |
酪氨酸 | Tyr | Y | UAC UAU |
例如,观察密码子表(表1)可以看出,密码子AGA、AGG、CGA、CGC、CGG和CGU都编码氨基酸精氨酸。因此,在本发明的核酸中在由密码子具体限定的精氨酸的每个位置上,此密码子可以被改变成上述任何一个相应的密码子,而不改变所编码的多肽。应当理解,RNA序列中的U对应于DNA序列中的T。
作为一个例子使用,对应于SEQ ID NO:1的核苷酸1-15的核酸序列(ATG ATT GAA GTC AAA(SEQ ID NO:826)),该序列的沉默变异包括AGT ATC GAG GTG AAG(SEQ ID NO:827);这两条序列编码氨基酸序列MIEVK(SEQ ID NO:828),其对应于SEQ ID NO:6的氨基酸1-5。
这种“沉默变异”是“保守性修饰变异”的一种,将在下文中讨论。技术人员将认识到,核酸中的每个密码子(除了AUG,其通常是甲硫氨酸的唯一密码子)可以用标准方法来修饰,以编码功能相同的多肽。因此,编码一种多肽的核酸的各个沉默变异隐含在任何所述序列中。本发明提供编码本发明的多肽的核酸序列的每一种可能变异,其可以通过选择基于可能的密码子的组合来制造。这些组合是按照应用于编码本发明的GAT同源物多肽的核酸序列中的标准三联遗传密码(例如在表1中所列举的)来进行。通过考虑序列以及遗传密码,特别地提供和描述了本文的每种核酸的所有这种变体。可以如在此所指出的方式制备任何变体。
一组两种或多种不同密码子在同样的环境中翻译时,它们都编码相同的氨基酸,在此被称作为“同义密码子”。如在此所描述,在本发明的一些方面中,将GAT多核苷酸工程化来在期望的宿主生物体中,例如在植物宿主应用优化的密码子。术语“优化的”或“最佳的”不限于最好的密码子的可能组合,而仅指作为整体该编码序列相对于衍生此编码序列的前体多核苷酸。因此,在一个方面中,本发明提供通过用同义密码子替换核苷酸序列中的至少一个亲本密码子来生产GAT多肽变体,相对于亲代密码子,该同义密码子是所需宿主生物如植物优选使用的。
特定核酸序列的“保守性修饰的变异”或者简单地说“保守性变异”是指那些编码相同的或基本上相同的氨基酸序列的核酸,或者,当该核酸不编码氨基酸序列时是指基本上相同的序列。熟练的技术人员将认识到,编码序列中改变、添加或缺失单个氨基酸或小比例氨基酸(通常少于5%、更加典型少于4%、2%或者1%或者更少)的个别取代、缺失或者添加是“保守性修饰的变异”,其中该改变导致氨基酸缺失、氨基酸添加或用化学上类似的氨基酸取代氨基酸。
提供功能性相似的氨基酸的保守性替代列表在本领域是公知的。表2列出了6组相互为“保守性替代”的氨基酸。
表2
保守性替代组
1 | 丙氨酸(A) | 丝氨酸(S) | 苏氨酸(T) |
2 | 天冬氨酸(D) | 谷氨酸(E) | |
3 | 天冬酰胺(N) | 谷氨酰胺(Q) | |
4 | 精氨酸(R) | 赖氨酸(K) | |
5 | 异亮氨酸(I) | 亮氨酸(L) | 甲硫氨酸(M)缬氨酸(V) |
6 | 苯丙氨酸(F) | 酪氨酸(Y) | 色氨酸(W) |
因此,所列举的本发明的多肽序列的“保守性替代的变异”包括用相同保守性替代组的保守性选择的氨基酸替代多肽序列中的氨基酸的小部分,典型地少于代5%、更加典型地少于2%和通常少于1%。因此本发明的多肽的保守性替代变异含有1个、2个、3个、4个、5个、6个、7个、8个、9个或10个用相同的保守性替代组的保守性替代变异进行的替代。
例如,在此确定的多肽如SEQ ID NO:6的保守性替代变异将含有上述六个组的“保守性替代”,替代发生在146个氨基酸的多肽的最多达7个残基(即,5%的氨基酸)上。
在另一个实施例中,如果四个保守性替代位于对应于SEQ ID NO:6的氨基酸21-30的区域,根据列举在表2中的保守性替代,则该区域的保守性替代变体的例子,
RPN QPL EAC M(SEQ ID NO:829),包括:
KPQ QPV ESC M(SEQ ID NO:830)和
KPN NPL DAC V(SEQ ID NO:831)等(在上述实施例,保守性替代用下划线表示)。在此的蛋白质序列的列表加上面的替代表格,提供所有保守性替代蛋白质的明确列表。
最后,加入不改变核酸分子的编码活性的序列,例如加入非功能性的或者非编码序列,是基础核酸的保守性变异。
技术人员将认识到,所公开的核酸构建体的许多保守性变异获得了功能相同的构建体。例如,如上所讨论,由于遗传密码子的简并性,“沉默替代”(即不会在所编码的多肽中产生改变的核酸序列中的替代)是每个编码氨基酸的核酸序列的隐含特征。类似地,氨基酸序列中的一个或几个氨基酸的“保守性氨基酸替代”被不同的具有高度相似特性的氨基酸替代,也很容易被鉴定为与所公开的构建体高度相似。各个所公开序列的这种保守性变体是本发明的一个特征。
特定核酸的非保守性修饰是那些取代任何不被表征为保守性替代的氨基酸的修饰。例如,任何跨越在表2中列举的六个组的边界的替代。这些包括用碱性或酸性氨基酸替代中性氨基酸(例如,用Asp、Glu、Asn或Gln替代Val、Ile、Leu或Met),用芳香氨基酸替代碱性或酸性氨基酸(例如,用Phe、Tyr或Trp取代Asp、Asn、Glu或Gln)或者任何其他不用类似的氨基酸替代一种氨基酸的替代
核酸杂交
当核酸结合时,发生“杂交”,通常在溶液中发生。核酸杂交是由于各种已知的物理化学力,例如氢键、溶剂排斥、碱基堆积等。大量对核酸杂交的教导可见于Tijssen(1993)Laboratory Techniquesin Biochenaistry and Molecular Biology--Hybridization withNucleic Acid Probes,第I部分,第2章,“Overview of principlesof hybridization and the strategy of nucleic acid probeassays”,(Elsevier,纽约(“Tijssen”)),以及Ausubel,同上文,Hames和Higgins(1995)Gene Probes 1,牛津大学出版社的IRL出版社,牛津,英格兰(“Haines和Higgins 1”)以及Hames和Higgins(1995)Gene Probes 2,牛津大学出版社的IRL出版社,牛津,英格兰(“Haines和Higgins 2”)提供关于合成、标记、缺失和量化DNA和RNA,包括寡核酸的详细内容。
核酸杂交试验中的“严紧性杂交洗涤条件”,例如Southern和northern杂交,是序列依赖性的,并且在不同环境参数下是不同的。大量对核酸杂交的教导可见于Tijssen(1993),同上文,以及可见于Hames和Higgins 1以及Hames和Higgins 2,同上文。
通常,处于本发明的目的,“高严紧性”杂交和洗涤条件被选择为,在特定离子强度和pH下,比特定序列的热溶化温度(Tm)低约5℃或更低(如下文指出,高度严紧条件也可以参考相当的术语)。Tm是50%的测试序列杂交到优选匹配的探针上的温度(在特定离子强度和pH下)。对于特定探针,选择与Tm相同的极其严紧条件。
核酸双链体的Tm表示在所给条件下双链体发生50%变性的温度,其表示核酸杂交的稳定性的直接测量值。因此,Tm对应于从螺旋到随机卷曲的转换的中点的温度,其取决于长链核苷酸的长度、核苷酸的组成和离子强度。
杂交后,通过一系列洗涤去除未被杂交的核酸物质,根据期望结果调整杂交严紧性。低严紧性洗涤条件(例如,采用较高浓度的盐和较低的温度)提高灵敏性,但是可能产生非特异性的杂交信号和高背景信号。高严紧条件(例如,采用较低的盐浓度和更为接近杂交温度的较高温度)降低背景信号,典型地只保留特异的信号。参见Rapley,R.和Walker,J.M.编辑,Molecular Biomethoods Handbook(Humana出版社公司1998)(下文称作“Rapley和Walker”),出于所有目的,在此完全引入作为参考。
用如下的方程1估计DNA-DNA双链体的Tm:
Tm(℃)=81.5℃+16.6(log10M)+0.41(%G+C)-0.72(%f)-500/n,
其中M是单价阳离子(通常为
)的摩尔浓度,(%G+C)是鸟嘌呤(G)和胞嘧啶(C)核苷的百分比,(%f)是缩甲醛化的百分比,而n是杂合体的核苷酸碱基的数量(即长度)。参见Rapley和Walker,同上文。
用如下的方程2估计DNA-DNA双链体的Tm:
Tm(℃)=79.8℃+18.5(log10M)+0.58(%G+C)-11.8(%G+C)2-0.56(%f)-820/n,
其中M是单价阳离子(通常为
)的摩尔浓度,(%G+C)是鸟嘌呤(G)和胞嘧啶(C)核苷的百分比,(%f)是缩甲醛化的百分比,而n是杂合体的核苷酸碱基的数量(即长度)。同样。
公式1和公式2通常只对长度约100-200个核苷以上的杂交双链体是精确的。同样。
短于50个核苷酸的核酸序列的Tm可按下式计算:
Tm(℃)=4(G+C)+2(A+T),
其中A(腺苷)、C、T(胸苷)和G是对应的核苷酸的数目。
互补残基超过100个的互补核酸在Southern或northern印迹的滤膜上杂交的严紧杂交条件的一个例子是42℃下,在含有1mg肝素的50%福尔马林中杂交过夜。严紧的洗涤条件的一个例子是在65℃下,用0.2×SSC洗涤15分钟(参见Sambrook,同上文,对SSC缓冲液的描述)。通常在高严紧洗涤之前进行低严紧洗涤,以除去背景探针信号。一种示例性的低严紧洗涤是在40℃下,用0.2×SSC洗涤15分钟。
通常,在特定杂交试验中,信噪比超过用不相关探针所观察到的2.5倍-5倍(或更高),表明检测到了特定的杂交。在本发明中,检测到两条序列之间至少发生严紧杂交,指示与如以在此所列举的序列表提供的本发明的核酸具有相对较高的结构相似性或同源性。
这里提到的“高严紧”条件被选定为,在确定的离子强度和pH下,比该特定序列的热融化温度(Tm)低5℃或更低。在高严紧条件下,可以确定与感兴趣的核苷酸序列(如“探针”)密切相关或相同的靶序列。低严紧条件适合于互补性较差的序列。参见,例如Rapley和Walker,同上文。
比较性杂交可用于鉴定本发明的核酸,这种比较性杂交法是区分本发明核酸的优选方法。对本发明中的两条核苷酸序列之间的高严紧杂交进行检测,指示与如以在此所列举的序列表提供的本发明的核酸具有相对较高的结构相似性或同源性。两条核苷酸序列之间发生高严紧杂交,证明了结构、核苷酸碱基组成、排布或顺序的相似或相同程度,其好于用严紧杂交条件所测得的结果。特别地,在本发明中,对高严紧杂交进行检测,指示与如以在此所列举的序列表提供的本发明的核酸具有相对较高的结构相似性或结构同源性(例如,核酸结构、碱基组成、排布或顺序)。例如,期望在严紧条件下,确定杂交到示例性核酸上的测试核酸。
因此,严紧杂交的一种衡量是,在高严紧条件下(或非常严紧的条件,或超高严紧杂交条件,或超超高严紧杂交条件下),与所列核酸(如,核酸序列SEQ ID NO:516、517、518、519、520、521、522、523、524、525、526、527、528、529、530、531、532、533、534、535、536、537、538、539、540、541、542、543、544、545、546、547、548、549、550、551、552、553、554、555、556、557、558、559、560、561、562、563、564、565、566、567、620、622、624、626、628、630、632、634、636、638、640、642、644、646、648、650、652、654、656、658、660、662、664、666、668、670、672、674、676、678、680、682、684、686、688、690、692、694、696、698、700、702、704、706、708、710、712、714、716、718、720、722、724、726、728、730、732、734、736、738、740、742、744、746、748、750、752、754、756、758、760、762、764、768、770、772、774、776、778、780、782、784、786、788、790、792、794、796、798、800、802、804、806、808、810、812、814、816、818、820、822、824、832、834、836、838、840、842、844、846、848、850、852、854、856、858、860、862、864、866、868、870、872、874、876、878、880、882、884、886、888、890、892、894、896、898、900、902、904、906、908、910、912、914、916、918、920、922、924、926、928、930、932、933、934、935、936、937、938、939、940、941、942、943、944、945、947、949、951和952,及其互补的多核苷酸序列)之一杂交的能力。对于任何测试核酸,可以容易地根据经验来确定严紧杂交(以及高严格、超高严紧、或超超高严紧杂交条件)和洗涤条件。例如,在确定高严紧杂交条件和洗涤条件时,逐渐提高杂交条件和洗涤条件(例如,提高杂交或洗涤时的温度、降低盐浓度、增加去污剂浓度和/或增加有机溶剂(如福尔马林)的浓度),直至符合所选定的一系列标准。例如,逐渐增加杂交条件和洗涤条件,直至含有选自SEQ ID NO:516、517、518、519、520、521、522、523、524、525、526、527、528、529、530、531、532、533、534、535、536、537、538、539、540、541、542、543、544、545、546、547、548、549、550、551、552、553、554、555、556、557、558、559、560、561、562、563、564、565、566、567、620、622、624、626、628、630、632、634、636、638、640、642、644、646、648、650、652、654、656、658、660、662、664、666、668、670、672、674、676、678、680、682、684、686、688、690、692、694、696、698、700、702、704、706、708、710、712、714、716、718、720、722、724、726、728、730、732、734、736、738、740、742、744、746、748、750、752、754、756、758、760、762、764、768、770、772、774、776、778、780、782、784、786、788、790、792、794、796、798、800、802、804、806、808、810、812、814、816、818、820、822、824、832、834、836、838、840、842、844、846、848、850、852、854、856、858、860、862、864、866、868、870、872、874、876、878、880、882、884、886、888、890、892、894、896、898、900、902、904、906、908、910、912、914、916、918、920、922、924、926、928、930、932、933、934、935、936、937、938、939、940、941、942、943、944、945、947、949、951和952,及其互补多核苷酸序列中的一条或多条核酸序列的探针,与完全匹配的互补目标(也是含有选自SEQ ID NO:516、517、518、519、520、521、522、523、524、525、526、527、528、529、530、531、532、533、534、535、536、537、538、539、540、541、542、543、544、545、546、547、548、549、550、551、552、553、554、555、556、557、558、559、560、561、562、563、564、565、566、567、620、622、624、626、628、630、632、634、636、638、640、642、644、646、648、650、652、654、656、658、660、662、664、666、668、670、672、674、676、678、680、682、684、686、688、690、692、694、696、698、700、702、704、706、708、710、712、714、716、718、720、722、724、726、728、730、732、734、736、738、740、742、744、746、748、750、752、754、756、758、760、762、764、768、770、772、774、776、778、780、782、784、786、788、790、792、794、796、798、800、802、804、806、808、810、812、814、816、818、820、822、824、832、834、836、838、840、842、844、846、848、850、852、854、856、858、860、862、864、866、868、870、872、874、876、878、880、882、884、886、888、890、892、894、896、898、900、902、904、906、908、910、912、914、916、918、920、922、924、926、928、930、932、933、934、935、936、937、938、939、940、941、942、943、944、945、947、949、951和952,及其互补多核苷酸序列中的一条或多条核酸序列的核酸)结合,其信噪比高于所观察到的探针与不匹配目标杂交结果至少约2.5倍,较好约高5倍或更高。这种情况下,不匹配的目标是与提交本申请时已存在于如GenBankTM公共数据库中的核酸(不是所附序列表中的那些)相对应的核酸。熟练的技术人员可以在GenBank中确定这种序列。例子包括登录号Z99109和Y09476。本领域普通技术人员可以在如GenBank中确定其它的此类序列。
当测试核酸以探针与完美匹配的互补目标杂交的至少1/2与探针杂交时,即信噪比至少为探针与靶核酸在其中完美匹配的探针与完美匹配的互补靶核酸结合的条件下杂交的信噪比的1/2时,则测试核酸特异地结合到探针核酸上,后一比所观察到的与登录号为Z99109和Y09476的任何非匹配多核苷酸杂交产生的信噪比至少高约2-10倍,偶而高20倍、50倍或更高。
超高严紧杂交和洗涤条件是这样的,其中杂交和洗涤条件的严紧性被提高,直至探针与完全匹配的互补靶核酸结合的信噪比比所观察到的与Genbank登录号为Z99109和Y09476的非匹配靶核酸的任何一条杂交的信噪比高至少10倍。在这种条件下,靶核酸以至少为完全匹配的互补靶核酸的信噪比的1/2的信噪比与探针杂交,即被认为在超高严紧条件下与探针结合。
类似地,通过逐步提高相关杂交试验的杂交和/或洗涤条件,可以确定甚至更高的严紧度。例如,其中提高杂交和洗涤条件的严紧性,直至探针和完美匹配的互补靶核酸结合的信噪比,至少比所观察到的与Genbank登录号为Z99109和Y09476的非匹配靶核酸的任何一种杂交的信噪比高10倍、20倍、50倍、100倍或500倍或更高。在这种条件下,以至少为完美匹配的互补靶核酸的信噪比的1/2与探针杂交的靶核酸,即被认为在超超高严紧条件下与探针结合。
在高、超高和超超高严紧条件下,与以SEQ ID NO:516、517、518、519、520、521、522、523、524、525、526、527、528、529、530、531、532、533、534、535、536、537、538、539、540、541、542、543、544、545、546、547、548、549、550、551、552、553、554、555、556、557、558、559、560、561、562、563、564、565、566、567、620、622、624、626、628、630、632、634、636、638、640、642、644、646、648、650、652、654、656、658、660、662、664、666、668、670、672、674、676、678、680、682、684、686、688、690、692、694、696、698、700、702、704、706、708、710、712、714、716、718、720、722、724、726、728、730、732、734、736、738、740、742、744、746、748、750、752、754、756、758、760、762、764、768、770、772、774、776、778、780、782、784、786、788、790、792、794、796、798、800、802、804、806、808、810、812、814、816、818、820、822、824、832、834、836、838、840、842、844、846、848、850、852、854、856、858、860、862、864、866、868、870、872、874、876、878、880、882、884、886、888、890、892、894、896、898、900、902、904、906、908、910、912、914、916、918、920、922、924、926、928、930、932、933、934、935、936、937、938、939、940、941、942、943、944、945、947、949、951和952为代表的核酸杂交的靶核酸,是本发明的一个特征。这些核酸的例子包括那些与所给定的核酸序列相比,具有一个或几个沉默或保守性核酸替代的核酸。
如果各种核酸所编码的多肽基本上是相同的,则在严紧条件下这些不相互杂交的核酸实质上仍是相同的。这种情况发生在,例如,当用遗传密码允许的最大密码子简并性产生某核酸的拷贝或产生抵抗SEQID NO:568、569、570、571、572、573、574、575、576、577、578、579、580、581、582、583、584、585、586、587、588、589、590、591、592、593、594、595、596、597、598、599、600、601、602、603、604、605、606、607、608、609、610、611、612、613、614、615、616、617、618、619、621、623、625、627、629、631、633、635、637、639、641、643、645、647、649、651、653、655、657、659、661、663、665、667、669、671、673、675、677、679、681、683、685、687、689、691、693、695、697、699、701、703、705、707、709、711、713、715、717、719、721、723、725、727、729、731、733、735、737、739、741、743、745、747、749、751、753、755、757、759、761、763、765、767、769、771、773、775、777、779、781、783、785、787、789、791、793、795、797、799、801、803、805、807、809、811、813、815、817、819、821、823、825、833、835、837、839、841、843、845、847、849、851、853、855、857、859、861、863、865、867、869、871、873、875、877、879、881、883、885、887、889、891、893、895、897、899、901、903、905、907、909、911、913、915、917、919、921、923、925、927、929、931、946、948、950、953、954、955、956、957、958、959、960、961、962、963、964、965、966、967、968、969、970、971和972中的一种或多种的一种或多种抗血清时,可以用已知核苷酸序列,包括Genbank登录号CAA70664的那些核苷酸序列所编码的多肽来减弱血清。下面是对免疫确定本发明多肽的进一步详细描述。此外,为了区分双链体不到100个核苷酸的序列,可以使用本领域普通技术人员已知的TMAC1杂交法。例如参见,Sorg,U.等人,Nucleic AcidRes.(1991年9月11日)19(17),出于所有目的,在此将其全文引入作为参考。
一方面,本发明提供一种核酸,该核酸包含核酸中的选自如下的独特亚序列:SEQ ID NO:516、517、518、519、520、521、522、523、524、525、526、527、528、529、530、531、532、533、534、535、536、537、538、539、540、541、542、543、544、545、546、547、548、549、550、551、552、553、554、555、556、557、558、559、560、561、562、563、564、565、566、567、620、622、624、626、628、630、632、634、636、638、640、642、644、646、648、650、652、654、656、658、660、662、664、666、668、670、672、674、676、678、680、682、684、686、688、690、692、694、696、698、700、702、704、706、708、710、712、714、716、718、720、722、724、726、728、730、732、734、736、738、740、742、744、746、748、750、752、754、756、758、760、762、764、768、770、772、774、776、778、780、782、784、786、788、790、792、794、796、798、800、802、804、806、808、810、812、814、816、818、820、822、824、832、834、836、838、840、842、844、846、848、850、852、854、856、858、860、862、864、866、868、870、872、874、876、878、880、882、884、886、888、890、892、894、896、898、900、902、904、906、908、910、912、914、916、918、920、922、924、926、928、930、932、933、934、935、936、937、938、939、940、941、942、943、944、945、947、949、951和952。与对应于Genbank登录号Z99109和Y09476的任何一种的核酸相比,这些独特亚序列是唯一的。可以将SEQ ID NO:516、517、518、519、520、521、522、523、524、525、526、527、528、529、530、531、532、533、534、535、536、537、538、539、540、541、542、543、544、545、546、547、548、549、550、551、552、553、554、555、556、557、558、559、560、561、562、563、564、565、566、567、620、622、624、626、628、630、632、634、636、638、640、642、644、646、648、650、652、654、656、658、660、662、664、666、668、670、672、674、676、678、680、682、684、686、688、690、692、694、696、698、700、702、704、706、708、710、712、714、716、718、720、722、724、726、728、730、732、734、736、738、740、742、744、746、748、750、752、754、756、758、760、762、764、768、770、772、774、776、778、780、782、784、786、788、790、792、794、796、798、800、802、804、806、808、810、812、814、816、818、820、822、824、832、834、836、838、840、842、844、846、848、850、852、854、856、858、860、862、864、866、868、870、872、874、876、878、880、882、884、886、888、890、892、894、896、898、900、902、904、906、908、910、912、914、916、918、920、922、924、926、928、930、932、933、934、935、936、937、938、939、940、941、942、943、944、945、947、949、951和952的任何一种与Genbank登录号Z99109、Y09476代表的一整套核酸或本申请提交日可以从公共数据库获得的其它相关序列进行序列比对,由此确定这些独特的亚序列。序列比对用设置了默认参数的BLAST算法进行。任何独特的亚序列都是有用的,例如,作为鉴定本发明核酸的探针。
类似地,本发明包括一种多肽,在该多肽中包含选自如下的独特亚序列:SEQ ID NO:568、569、570、571、572、573、574、575、576、577、578、579、580、581、582、583、584、585、586、587、588、589、590、591、592、593、594、595、596、597、598、599、600、601、602、603、604、605、606、607、608、609、610、611、612、613、614、615、616、617、618、619、621、623、625、627、629、631、633、635、637、639、641、643、645、647、649、651、653、655、657、659、661、663、665、667、669、671、673、675、677、679、681、683、685、687、689、691、693、695、697、699、701、703、705、707、709、711、713、715、717、719、721、723、725、727、729、731、733、735、737、739、741、743、745、747、749、751、753、755、757、759、761、763、765、767、769、771、773、775、777、779、781、783、785、787、789、791、793、795、797、799、801、803、805、807、809、811、813、815、817、819、821、823、825、833、835、837、839、841、843、845、847、849、851、853、855、857、859、861、863、865、867、869、871、873、875、877、879、881、883、885、887、889、891、893、895、897、899、901、903、905、907、909、911、913、915、917、919、921、923、925、927、929、931、946、948、950、953、954、955、956、957、958、959、960、961、962、963、964、965、966、967、968、969、970、971和972。在此,与对应于Genbank登录号CAA70664的多肽相比,这些独特的亚序列是唯一的。同时,将这些多肽与Genbank登录号CAA70664代表的序列进行比对。应当注意的是,如果该序列对应于非翻译序列,如假基因,则只要将核酸序列在计算机芯片中(insilico)翻译成氨基酸序列就可以产生相应的多肽,其中选择阅读框,以与同源GAT多核苷酸的阅读框相对应。
本发明还提供在严紧条件下杂交到独特的编码寡核苷酸上的靶核酸,该寡核苷酸编码多肽中选自如下的独特亚序列:SEQ ID NO:568、569、570、571、572、573、574、575、576、577、578、579、580、581、582、583、584、585、586、587、588、589、590、591、592、593、594、595、596、597、598、599、600、601、602、603、604、605、606、607、608、609、610、611、612、613、614、615、616、617、618、619、621、623、625、627、629、631、633、635、637、639、641、643、645、647、649、651、653、655、657、659、661、663、665、667、669、671、673、675、677、679、681、683、685、687、689、691、693、695、697、699、701、703、705、707、709、711、713、715、717、719、721、723、725、727、729、731、733、735、737、739、741、743、745、747、749、751、753、755、757、759、761、763、765、767、769、771、773、775、777、779、781、783、785、787、789、791、793、795、797、799、801、803、805、807、809、811、813、815、817、819、821、823、825、833、835、837、839、841、843、845、847、849、851、853、855、857、859、861、863、865、867、869、871、873、875、877、879、881、883、885、887、889、891、893、895、897、899、901、903、905、907、909、911、913、915、917、919、921、923、925、927、929、931、946、948、950、953、954、955、956、957、958、959、960、961、962、963、964、965、966、967、968、969、970、971和972,其中,与对应于对照多肽的任何一种的多肽相比,这种独特的亚序列是唯一的。如上所述确定这些独特的亚序列。
在一个实施例中,选择严紧条件,使得与该编码寡核苷酸完美互补的寡核苷酸与该编码寡核苷酸杂交,其信噪比比该完美互补的寡核苷酸与对应于对照多肽的任何一种的对照核酸杂交的信噪比高2.5-10倍,优选至少高5-10倍。选择条件,以使在所用的特定分析中观察到较高的信噪比,例如为约15倍、20倍、30倍、50倍或更高。在这个实施例中,与对照核酸与该编码寡核苷酸的杂交相比,靶核酸以至少高2倍的信噪比与该独特的编码寡核苷酸杂交。另外,可以选择较高的信噪比,例如,约2.5倍、5倍、10倍、20倍、30倍、50倍或更高。具体的信号取决于相关分析中所用的标记,如荧光标记、比色标记、放射性标记等。
载体、启动子和表达系统
本发明还包括含有如上广泛描述的核酸序列中的一条或多条的重组构建体。这种构建体包括载体,如质粒、粘粒、噬菌体、病毒、细菌的人造染色体(BAC)、酵母菌人造染色体(YAC)等,其中以正向或反向插入了本发明的核酸序列。在这个实施方案的优选方面,这种构建体还包括调控序列,包括,例如可操作地连接到该序列上的启动子。大量合适的载体和启动子是本领域技术人员已知的,并且可以购买得到。
如先前所讨论,普通教科书中描述了可用于本发明的分子生物技术,包括载体、启动子的使用和许多其它相关主题,这些教科书包括Berger和Kimmel,Guide to Molecular Cloning Techniques,Methods in Enzymology第152卷,学术出版社公司,圣地亚哥,加利福尼亚州(Berger);Sambrook等人,Molecular Cloning-ALaboratory Manual(第2版),1-3卷,冷泉巷实验室,冷泉巷,纽约,1989(“Sambrook”)以及Current Protocols in MolecularBiology,F.M.Ausubel等人编辑,Current Protocols,GreenePublishing Associates公司和John Wiley & Sons公司的合资公司,(1999增补)(“Ausubel”)。足以指导熟练技术人员通过体外扩增法,包括聚合酶链式反应(PCR)、连接酶链式反应(LCR)、Qβ-复制酶扩增和其他RNA聚合酶介导的技术(例如NASBA),来例如生产本发明的同源核酸的技术例子,可见于Berger,Sambrook和Ausubel以及Mullis等人(1987)美国专利4,683,202;Innis等人编辑(1990),PCR Protocols A Guide to Methods and applications(学术出版社公司,圣地亚哥,加利福尼亚州)(“Innis”);Arnheim&Levinson(1990年10月1日)C&EN 36-47;The Journal Of NIHResearch(1991)3:81-94;Kwoh等人(1989)Proc.Natl.Acad.Sci.USA 86:1173;Guatelli等人(1990)Proc.Natl.Acad.Sci.USA 87:1874;Lomell等人(1989)J.Clin.Chem.35:1826;Landegren等人(1988)Science 241:1077-1080;Van Brunt(1990)Biotechnology 8:291-294;Wu和Wallace(1989)Gene 4:560;Barringer等(1990)Gene 89:117;以及Sooknanan和Malek(1995)Biotechnology 13:563-564。体外克隆扩增核酸的改进方法被描述在Wallace等人的美国专利5,426,039中。通过PCR扩增大型核酸的改进方法总结在Cheng等人(1994)Nature 369:684-685和其中引用的参考文献中,其中产生了最高达40kb的PCR扩增子。技术人员将会认识到,几乎任何RNA可转变为双链DNA,以适合限制性消化、PCR扩增并用逆转录酶和聚合酶进行测序。参见,Ausbel、Sambrook和Berger,同上文。
本发明还涉及用本发明载体(如本发明的克隆载体或本发明的表达载体)转导(转化或转染)的工程化宿主细胞,以及用重组技术生产本发明的多肽。此载体可以是,例如质粒、病毒颗粒、噬菌体等。工程化的宿主细胞可以被培养在常规营养基中,该营养基被改进以活化启动子、选择转化体或扩增GAT同系物基因。培养条件,如温度、pH等是以前用于被选择用来表达的宿主细胞的培养条件,并且对于本领域技术人员来说是显而易见的,并且在本文引用的参考文献中,包括如Sambrook、Ausubel和Berger,以及如Freshney(1994)Cultureof Animal Cells,A Manual of Basic Technique,第三版,(Wiley-Liss,纽约)和其中引用的参考文献。
可以在植物、酵母、真菌等非动物细胞中生产本发明的GAT多肽。除了Sambrook、Ausubel和Berger外,有关非动物细胞培养的细节可以参见Payne等(1992)Plant Cell and Tissue Culture in LiquidSystems(John Wiley & Sons公司,纽约,纽约州);Gamborg和Phillips(编辑)(1995)Plant Cell,Tissue and OrganCulture:Fundanmental Methods/Springer Lab Manual,(Springer-Verlag,柏林)以及Atlas和Parks(编辑)The Handbookof Microbiological Media(1993)CRC出版社,伯克莱屯,佛罗里达州。
可以将本发明的多核苷酸掺入到适合表达多肽的任何一种表达载体中。合适的载体包括染色体、非染色体和合成的DNA序列,如SV40的衍生物;细菌质粒;噬菌体DNA;杆状病毒;酵母菌质粒;来源于质粒和噬菌体DNA的组合的载体;病毒DNA、如牛痘苗、腺病毒、禽痘病毒、拟狂犬病毒、腺病毒、腺相关病毒、逆转录病毒和许多其他病毒。可采用能将遗传物质转导入细胞的任何载体,如需要复制,则为在有关宿主中可复制、有活力的载体。
当结合到表达载体中时,本发明的多核苷酸被可操作地连接到适当的转录控制序列(启动子)上,引导mRNA合成。特别适合用于转基因植物中的这种转录调控序列的例子包括花椰菜花叶病毒(CaMV)、玄参花叶病毒(FMV)、草莓纹理带病毒(SVBV)启动子,这些在美国临时专利申请60/245354中描述了。已知能调控基因在真核或原核细胞或其他病毒中表达的和可用于本发明的某些实施方案中的其他启动子包括SV40启动子、大肠杆菌lac或trp启动子、噬菌体λPL启动子。表达载体任选地含有翻译启始的核糖体结合位点和转录终止子,如PinII。该载体还任选地含有用于增强表达的适当序列,如增强子。
此外,本发明的表达载体任选地含有一种或多种可选择的标记基因,提供用以选择被转化的宿主细胞的表型性状。通常,可选择的标记基因编码抗生素或者除草剂抗性。合适的基因包括编码对抗生素奇霉素或链霉素具有抗性的基因(例如,aada基因)、编码链霉素抗性的链霉素磷酸转移酶(SPT)基因、编码卡那霉素或遗传霉素抗性的新霉素磷酸转移酶(NPT II)基因、编码潮霉素抗性的潮霉素磷酸转移酶(HPT)基因。其他的可选择的标记基因包括二氢叶酸还原酶或用于真核细胞培养的新霉素抗性,以及用于大肠杆菌培养的四环素或氨苄青霉素抗性。
编码对除草剂抗性的合适基因包括那些抑制乙酰乳酸合酶(ALS)的作用的基因,特别是编码对磺酰脲型除草剂的抗性的基因(例如含有产生这种抗性的突变的乙酰乳酸合酶(ALS),特别是S4和/或Hra突变)、抑制谷氨酸盐合酶的作用的那些基因,例如草酊磷或者basta(例如bar基因),或者本领域知晓的其他这种基因。bar基因编码对除草剂basta的抗性,而ALS基因编码对除草剂氯磺隆(chlorsulfuron)的抗性。在一些情况下,被改变的GAT基因用作可选择标记。
本发明的载体可用于转化适当的宿主,使该宿主表达本发明的蛋白质或多肽。合适的表达宿主的例子包括:细菌细胞,如大肠杆菌、枯草芽孢杆菌、链霉菌和沙门伤寒杆菌;真菌细胞,如酿酒酵母、毕赤巴斯德酵母和脉胞菌;昆虫细胞,例如果蝇和草地夜蛾;哺乳动物细胞,如CH0、COS、BHK、HEK293或Bowes黑素瘤;或者植物细胞或外植体等。应当理解,并非所有的细胞或细胞系都必须能够产生功能完全的GAT多肽;例如可能生成GAT多肽的抗原性片段。本发明不受所用的宿主细胞的限制。
在细菌系统中,可以根据GAT多肽的使用意图来选择多种表达载体。例如,当由于商业生产或者用于诱导抗体,需要大量GAT多肽或其片段时,引导高水平表达易于纯化的融化蛋白的载体是期望的。这种载体包括但不限于,多功能的大肠杆菌克隆和表达载体,如BLUESCRIPT(Stratagene),其中,可以将GAT多肽编码序列在读框内与氨基末端为Met和随后β-半乳糖苷酶的7个残基的序列一起连接到载体中,生成了一种杂合蛋白;pIN载体(Van Heeke & Schuster(1989)J Biol Chem 264:5503-5509);pET载体(Novagen,MadisonWI);等等。
类似地,在酿酒酵母中,有许多含组成型或诱导型启动子的载体可用来生产本发明的GAT多肽,这些启动子例如是α因子、醇氧化酶和PGH。综述可以参见Ausubel等人(同上)和Grant等人(1987),Methods in Emzymology 153:516-544。
在哺乳类宿主细胞中,可以使用包括以病毒为基础的系统的各种表达系统。当将腺病毒用作表达载体时,例如,可将GAT多肽的编码序列可操作地连接到由晚期启动子和三联前导序列组成的腺病毒转录/翻译复合体中。在该病毒基因组的非必需E1或E3区插入GAT多肽编码区,将生成能够在被感染的宿主细胞中表达GAT的活病毒(Logan和Shenk(1984)Proc Natl Acad Sci USA 81:3655-3659)。此外,可以使用转录增强子,如罗斯肉瘤病毒(RSV)增强子,以增加在哺乳动物宿主细胞中的表达。
类似地,在植物细胞中,可以驱动被整合到植物染色体中的转基因表达,或由附加体或病毒核酸在胞质中进行表达。对于稳定整合的转基因,常常期望提供能够驱动本发明GAT多核苷酸的组成型或诱导型表达的序列,例如,使用病毒,如CaMV,或植物来源的调控序列。大量植物来源的调控序列已经被描述,包括以组织特异性方式引导表达的序列,如TobRB7、patatin B33、BRP基因启动子、rbcS-3A启动子等。或者,可以通过瞬时表达植物病毒载体(如,TMV、BMV等)的外源序列,来实现高水平表达。通常,组成型表达本发明GAT多核苷酸的转基因植物是优选的,并选择其调控序列,以确保组成性稳定表达GAT多肽。
用于在高等植物中表达核酸的典型载体是本领域熟知的,包括来源于Rogers等人(1987)Mets.Enzymol.153:253-277中描述的根瘤农杆菌的肿瘤诱导型(
)质粒的载体。可用于此的示例性根瘤农杆菌载体为Schardl等人(1987)Gene 61:1-11和Berger等人(1989)Proc.Nat′l.Acad.Sci.U.S.A.86:8402-8406描述的质粒pKYLX6和pKYLX7。另一种可用于此的载体为质粒pBI101.2,可以从ClontechLaboratories公司(Palo Alto,加利福尼亚州)购买得到。可用作载体的各种植物病毒在本领域是已知的,包括花椰菜花叶病毒(CaMV)、双生病毒(geminivirus)、雀麦草花叶病毒和烟草花叶病毒。
在本发明的一些实施方案中,制备了适合转化植物细胞的GAT多肽构建体。例如,将所需的GAT多核苷酸掺入到重组表达盒中,以便于将该基因导入植物,并随后表达所编码的多肽。表达盒通常包括可操作地连接到启动子序列和其它转录和翻译起始调控序列上的GAT多核苷酸或其功能片段,这些控制序列指导了该序列在被转化植物的特定组织(例如,整个植物、叶、种子)中的表达。
例如,可以使用强或弱的组成型植物启动子,它将引导GAT多肽在植物的所有组织中表达。在大多数环境条件下和在发育或细胞分化阶段中,这种启动子都有活性。组成型启动子的例子包括花椰菜花叶病毒(CaMV)35S转录起始区、来源于根瘤农杆菌T-DNA的1’-或2’-启动子、泛素1启动子、Smas启动子、肉桂醇脱氢酶启动子(美国专利5,683,439)、Nos启动子、pEmu启动子、核酮糖-1,5-二磷酸羧化酶-加氧酶启动子、GRP1-8启动子和技术人员知晓的来自各种植物基因的其他转录起始区。当GAT多核苷酸过度表达对植物有害或不需要时,通过阅读本文,技术人员将懂得可以使用弱的组成型启动子,以低水平表达。当高水平表达对植物无害时,可以使用强启动子,如t-RNA或其它pol III启动子,或强pol II启动子,如花椰菜花叶病毒启动子。
可替代地,植物启动子在环境控制之下。这种启动子在此被称为“诱导型”启动子。可通过诱导型启动子来进行转录的环境条件的例子包括病原体攻击、厌氧条件或光照的存在。特别地,诱导型启动子的例子为由缺氧或者冷刺激诱导的Adh1启动子、由热应激诱导的Hsp70启动子、由光照诱导的PPDK启动子。化学诱导的启动子也是有用的。
用于本发明的启动子可以是“组织特异的”以及,这样,处于发育控制之下,其中多核苷酸只在某些组织,如叶、根、果实、花和/或种子中表达。示例性的启动子是花药特异性启动子5126(美国专利5,689,049和5,689,051)。种子优选的启动子的例子包括,但是不限于,27kD的γ玉米蛋白启动子和蜡状启动子,Boronat等人(1986)Plant Sci.47,95-102;Reina等人(1990)Nucleic AcidsRes.18(21):6426;以及Kloesgen等人(1986)Mol.Gen.Genet.203:237-244。在胚胎、果皮和胚乳中表达的启动子被公开在美国专利申请1998年8月20日申请的60/097,233和1998年8月28日申请的60/098,230中。这两份专利公开的内容在此完全引入作为参考。在实施方案中,在构建体中引入了一种或多种植物系统的内源核酸序列,可以使用这些基因的内源启动子(或其变体),以在转基因植物中指导这些基因的表达。也可以用组织特异性启动子,来指导异源多核苷酸的表达。
总之,用于植物表达盒中的特定启动子取决于特定应用。可以使用异源的或非异源的(内源的)启动子,来指导本发明的核酸的表达。这些启动子可以用在如表达盒中,来驱动反义核酸的表达,以在期望的组织中降低、升高或改变本发明的蛋白质的浓度和/或组成。许多在植物细胞中指导转录的启动子中的任何一种都是合适的。启动子可以是组成型或诱导型的。除了上述启动子外,在植物中起作用的细菌来源的启动子包括章鱼碱合酶启动子、胭脂碱合酶启动子和其它来源于天然Ti质粒的启动子(参见Herrara-Estrella等人(1993)Nature303:209-213)。病毒启动子包括花椰菜花叶病毒的35S和19S RNA启动子(Odell等(1985)Nature 313:810-812)。其它的植物启动子包括核酮糖-1,3-二磷酸羧化酶小亚基启动子和菜豆蛋白启动子。也可以使用来自E8基因和其它基因的启动子序列。E8启动子的分离和序列被详细描述在Deikman和Fischer(1988)EMBO J.7:3315-3327中。
为了鉴定候选启动子,对基因组克隆的5’部分作启动子序列的序列特征分析。例如,启动子序列元件包括TATA框共有序列(TATAAT),它通常在转录起始位点上游的20-30个碱基对处。在植物中,在TATA框上游,在-80到-100的位置上,通常有启动子元件和一系列围绕三核苷酸G(或T)的腺苷酸,正如Messing等人(1983)GeneticEngineering in Plants,Kosage等人(编辑),第221-227页所述。
在制备本发明的多核苷酸构建体,如载体时,也可以使用启动子以外的序列和共连接的多核苷酸。如果需要正常的多肽表达,可以在GAT-编码区3’-末端包含多聚腺苷酸化区域。例如,多聚腺苷酸化区域可来源于各种植物基因,或来自T-DNA。将要被添加的3’端序列可以来源于如胭脂碱合酶或者章鱼碱合酶基因,或者可替代地来源于另一种植物基因,或者较不优选地来源于任何其他的真核基因。
将内含子序列添加到编码序列的5’非翻译区或者部分编码序列中,以增加所积累的成熟信息的含量。可以参见如Buchman和Berg(1988)Mol.Cell Biol.8:4395-4405以及Callis等人(1987)Genes Dev.1:1183-1200。玉米内含子Adh1、内含子1、2和6以及黄铜-1内含子的用途是本领域已知的。通常参见Freeling和Walbot编辑的(1994)The Maize Handbook(Springer,纽约),第116章。
此构建体还可以包括赋予植物细胞以可选择表型的标记基因。例如,这种标记可以是编码对杀虫剂的耐受性、对特定抗生素的耐受性,如对卡那霉素、G418、博来霉素和潮霉素的耐受性,或对除草剂的耐受性,例如对氯磺隆或膦丝菌素(除草剂双丙氨膦和Basta中的活性成分)的耐受性。
特定的起始信号有助于本发明的GAT多核苷酸编码序列的有效翻译。这些信号包括,例如,ATG起始密码子和毗邻序列。如果是GAT多肽编码序列,则将其起始密码子和上游序列插入合适的表达载体中,不需要其它的翻译控制信号。然而,如果只插入了编码序列(如成熟蛋白编码序列)或其部分,则必须提供包括起始密码子的外源转录控制信号。此外,起始密码子必需位于正确的阅读框中,以确保整个插入片段的转录。外源转录元件和起始密码子可以是各种来源的,可以是天然的或合成的。可以在所使用的细胞系统中加入适合的增强子,以提高表达效率(Scharf D等人(1994)Results Probl Cell Differ20:125-62;Bittner等人(1987)Methods in Enzymol 153:516-544)。
分泌/定位序列
本发明的多核苷酸还可以,例如在阅读框内被融合到编码分泌/定位序列的核酸上,以将多肽表达靶向所期望的宿主细胞的细胞区腔室、膜、或细胞器中,或引导多肽分泌到周质间隙中或分泌到细胞培养介质中。这些序列是技术人员已知的,包括分泌前导肽、细胞器靶向肽(如核定位序列、ER保留信号、线粒体转运序列、叶绿体转运序列)、膜定位/锚定序列(如停止转移序列、GPI锚定序列)等。
在优选的实施方案中,本发明的多核苷酸在阅读框内融合了N-末端叶绿体转移序列(或叶绿体转运肽序列),该转运序列来源于编码通常被靶向叶绿体的多肽的基因。这种序列通常富含丝氨酸和苏氨酸;缺乏天冬氨酸、谷氨酸和酪氨酸;并且通常含有富含正电荷氨基酸的中心区域。
表达宿主
在另一个实施方案中,本发明涉及含有上述构建体的宿主细胞。这种宿主细胞可以是真核细胞,如哺乳动物细胞、酵母细胞或植物细胞,或者该宿主细胞可以是原核细胞,如细菌细胞。可通过磷酸钙转染、DEAE-葡聚糖转染、电穿孔或其它常规技术,将该构建体导入宿主细胞中(Davis等人,Basic Methods in Molecular Biology)。
任选地对宿主细胞调节所插入的序列的表达或以期望的形式加工所表达的蛋白质的能力进行选择。蛋白质的这种修饰包括但不限于乙酰化、羧化、糖基化、磷酸化、脂质化和酰化。切割蛋白质的“前”或“前原”形式的翻译后加工对于正确插入、折叠和/或功能也是重要的。不同的宿主细胞,如大肠杆菌、杆菌属、酵母或哺乳动物细胞,如CHO、Hela、BHK、MDCK、293、WI38等,具有特定的有关例如翻译后活性的细胞机理和特征机制,可以对此进行选择,以确保如所期望地修饰和加工所导入的外源蛋白质。
为了长期、高产量地生产重组蛋白,可以使用稳定的表达系统。例如,用含有病毒性复制起点或外源表达元件和可选标记基因的表达载体来转导稳定地表达本发明多肽的植物细胞、外植体或组织,如芽、叶盘。在导入载体后,可以让细胞在富集培养基中生长与细胞类型相适应的一段时间,如让细菌细胞生长1小时或几小时,让植物细胞生长1-4天,让一些植物外植体生长2-4周,然后换成选择性培养基。可选标记的目的是赋予对选择的抗性,它的存在使成功表达导入序列的细胞可以生长并被回收。例如,可以就除草剂、草甘膦的抗性直接选择表达本发明多肽的转基因植物。例如可采用适合细胞类型的组织培养技术,来繁殖来源于稳定转化的外植体的抗性胚。
将使用编码本发明多肽的核苷酸序列转化的宿主细胞任选地培养在适合该编码蛋白质表达并从细胞培养物中回收的条件下。重组细胞生产的蛋白质或其片段可以是分泌的、膜结合的或含在细胞内,这取决于所用的序列和/或载体。本领域的技术人员将理解,可以设计含有本发明GAT多核苷酸的表达载体,使其具有指导成熟多肽分泌通过原核或真核细胞膜的信号序列。
其它多肽序列
本发明的多核苷酸还可以包括在阅读框内融合到标记序列上的编码序列,标记序列例如便于纯化所编码的多肽。这种方便纯化的结构域包括但不限于,金属螯合肽,如允许在固定化的金属上纯化的组氨酸-色氨酸组件、结合谷胱甘肽的序列(如GST)、血凝素(HA)尾(对应于来源于流感血凝素蛋白的表位;Wilson等(1984)Cell 37:767)、麦芽糖结合蛋白序列、用于FLAGS延伸/亲和纯化系统的FLAG表位(Immunex公司,西雅图,华盛顿州)等。在纯化结构域和GAT同系物序列之间包含蛋白酶可切割的多肽接头序列,对于方便纯化是有用的。用于本文所述组合物和方法中的一个表达载体,可用于表达含有本发明多肽的融合蛋白,该多肽被融合到通过肠激酶切割位点分开的多聚组氨酸区域上。组氨酸残基便于在IMIAC(固定的金属离子亲和层析,如Porath等(1992)Protein Expression and Purification 3:263-281)所述)上进行纯化,而肠激酶切割位点提供了一种从融合蛋白上分离GAT同系物多肽的手段。也可以用pGEX载体(Promega;麦迪逊,威斯康星州)以与胱甘肽S-转化酶(GST)的融合蛋白的形式来表达外源多肽。总之,这种融合蛋白是可溶的,并可以通过吸附于配体-琼脂糖珠(如在GAT-融合时使用谷胱甘肽-琼脂糖),然后在存在游离配体时进行洗脱,这样可以容易地从溶解的细胞中将其纯化。
多肽的生产和回收
在转导合适的宿主菌株并使宿主菌株生长至合适的细胞密度后,用合适的手段(如改变温度或化学诱导)诱导所选的启动子,将细胞再培养一段时间。通常通过离心收获细胞,用物理或化学方法破裂,并将所得粗制提取物用于进一步纯化。可以用任何常规的方法破裂用于表达蛋白质的微生物细胞,这些方法包括冷冻-解冻循环、超声波、机械破碎或使用细胞溶解剂或其它方法,这些都是本领域技术人员熟知的。
值得一提的是,有许多关于培养和生产许多细胞,包括细菌、植物、动物(尤其是哺乳动物)和古细菌来源的细胞的参考文献。参见例如Sambrook、Ausubel和Berger(都同上文),以及Freshney(1994)Culture of Animal Cells:A Manual of Basic Technique,第三版,(Wiley-Liss,纽约)和其中引用的参考文献;Doyle和Griffiths(1997)Mammalian Cell Culture:Essential Techniques(JohnWiley & Sons,纽约州);Humason(1979)Animal Tissue Techniques,第四版(W.H.Freeman和Company);以及Ricciardelli等人(1989)In vitro Cell Dev.Biol.25:1016-1024。有关植物细胞的培养和再生,可以参见Payne等人(1992)Plant Cell and Tissue Culturein Liquid Systems(John Wiley & Sons公司,纽约,纽约州);Gamborg和Phillips(编辑)(1995)Plant Cell,Tissue and OrganCulture:Fundanmental Methods/Springer Lab Manual(Springer-Verlag,柏林);Jones编辑(1994)Plant Gene Transferand Expression Protocols(Humana出版社,Towata,新泽西州);以及Croy编辑(1993)Plant Molecular Biolgy(Bios Scientific出版社,牛津,英国),ISBN 0 12 198370 6。细胞培养介质通常列在Atlas和Parks编辑(1993)The Handbook of MicrobiologicalMedia(CRC出版社,伯克莱屯,佛罗里达州)。关于细胞培养的其它信息可以在以下市售文献中找到,如Sigma-Aldrich公司(圣路易斯,密苏里州)的Life Science Research Cell Culture Catalogue(1998)(“Simga-LSRCCC”)和例如,同样来自Sigma-Aldrich公司(圣路易斯,密苏里州)的The Plant culture Catalogue及增补本(1997)(“Simga-PCCS”)。其它有关植物细胞转化和制造转基因植物的详述可以在下面找到。
用任何本领域熟知的方法,可以从重组细胞培养物中回收并纯化本发明的多肽,这些方法包括硫酸铵或乙醇沉淀、酸提取、阴离子或阳离子交换层析、磷酸纤维素层析、疏水反应层析、亲和层析(如使用本文提到的任何标记系统)、羟基磷灰石层析以及凝集素层析。如果需要,在完成成熟蛋白的构型时采用蛋白质再折叠步骤。最后,在最终的纯化步骤中使用高效液相色谱(HPLC)。除了上述参考文献外,各种纯化方法是本领域熟知的,包括例如在以下文献中提到的方法,Sandana(1997)Bioseparation of Proteins,学术出版社公司;Bollag等(1996)Protein Methods,第二版(Wiley-Liss,纽约州);Walker(1996)The Protein Protocols Handbook(Humuna出版社,新泽西州);Harris和Angal(1990)Protein PurificationApplications:A Practical Approach(牛津IRL出版社,牛津,英国);Scopes(1993)Protein Purification:Principles andPractice第三版(Springer Verlag,纽约州);Janson和Ryden(1998)Protein Purification:Priciples,High ResolutionMethods and Applications,第二版(Wiley-VCH,纽约州);以及Walker(1998)Protein Protocols on CD-ROM(Humana出版社,新泽西州)。
在某些情况下,需要以适合工业和/或商业应用的大规模来生产本发明的GAT多肽。在这种情况下,可采用批量发酵程序。简单地说,可将GAT多核苷酸或编码本发明GAT多肽的其它核酸克隆进表达载体中,该多核苷酸包括516、517、518、519、520、521、522、523、524、525、526、527、528、529、530、531、532、533、534、535、536、537、538、539、540、541、542、543、544、545、546、547、548、549、550、551、552、553、554、555、556、557、558、559、560、561、562、563、564、565、566、567、620、622、624、626、628、630、632、634、636、638、640、642、644、646、648、650、652、654、656、658、660、662、664、666、668、670、672、674、676、678、680、682、684、686、688、690、692、694、696、698、700、702、704、706、708、710、712、714、716、718、720、722、724、726、728、730、732、734、736、738、740、742、744、746、748、750、752、754、756、758、760、762、764、768、770、772、774、776、778、780、782、784、786、788、790、792、794、796、798、800、802、804、806、808、810、812、814、816、818、820、822、824、832、834、836、838、840、842、844、846、848、850、852、854、856、858、860、862、864、866、868、870、872、874、876、878、880、882、884、886、888、890、892、894、896、898、900、902、904、906、908、910、912、914、916、918、920、922、924、926、928、930、932、933、934、935、936、937、938、939、940、941、942、943、944、945、947、949、951和952的任何之一。例如,Widner等人的美国专利5,955,310“在杆菌细胞中生产多肽的方法”中描述了一种载体,其带有串联的增强子和可操作地连接到多肽编码序列上的稳定序列。将感兴趣的多核苷酸插入载体后,将该载体转化入细菌宿主,如枯草芽孢杆菌菌株PL180lIIE(amyE、apr、npr、spoIIE::Tn917)中。在芽孢杆菌细胞中引入表达载体,可采用如原生质体转化(例如参见Chang和Cohen(1979)Mol.Gen.Genet.168:111)、采用感受态细胞(例如参见Young和Spizizin(1961)J.Bacteriol.81:823,或Dubnau和Davidoff-Abelson(1971)J.ofMol.Bio.56:209)、电穿孔(例如参见Shigekawa和Dower(1998)Biotechniques 6:742)、或通过偶联(例如参见Koehler和Thorne(1987)J.of Bacteriol.169:5271),以及Ausubel、Sambrook、Berger(都同上文)来实施。
用本领域已知的方法,将转化的细胞培养在适合生产多肽的营养培养基中。例如,可以在合适的培养基和多肽能够被表达和/或分离的条件下,在实验室或工业发酵罐中,通过摇床培养、小规模或大规模发酵(包括连续发酵、分批发酵、分批补料发酵或固态发酵)来培养细胞。采用本领域已知的程序,在含有碳源和氮源以及无机盐的合适营养培养基中进行培养。合适的培养基可从商业供应商获得,或按照所公布的组成(如美国典型培养物保藏中心的目录中)制备。可以从培养基中直接回收分泌的多肽。
可用本领域已知的方法分离所生成的多肽。例如,可用常规方法从营养培养基中分离多肽,这些方法包括但不限于离心、过滤、提取、喷雾干燥、蒸发或沉淀。然后可以用本领域已知的各种方法,来进一步纯化分离的多肽,其中包括但不限于层析法(如离子交换、亲和、疏水、层析聚焦和排阻)、电泳法(如制备型等电聚焦)、差别性溶解(如硫酸铵沉淀)或提取(例如参见Bollag等(1996)ProteinMethods第二版(Wiley-Liss,纽约州);Walker(1996)The ProteinProtocols Handbook(Humana出版社,新泽西州)。
也可以使用无细胞转录/翻译系统,用本发明的DNA或RNA来生产多肽。几个这种系统可以购买得到。有关体外转录和翻译过程的一般介绍可见于Tymms(1995)In vitro Transcription and TranslationProtocols:Methods in Molecular Biology(Gerland出版社,纽约州),第37卷。
用于序列重组的底物和模式
除了用于如Ausubel、Berer和Sambrook列举的标准克隆方法中来生产具有所需特性的其它GAT多核苷酸和多肽的用途外,本发明的多核苷酸任选地被用作各种产生多样性的方法的底物,多样性过程例如是突变、重组和递推重组反应。各种产生多样性的方法是可获得的,并且在本领域中有描述。这些方法可以单独使用,和/或联合使用以生产多核苷酸或一组多核苷酸的一种或多种变体,以及所编码蛋白的变体。无论是单独还是联合,这些过程提供了产生多样化的多核苷酸和多核苷酸组(例如包括多核苷酸文库)的强有力的、广泛适用的方法,这些多样化的多核苷酸和多核苷酸组可用于例如工程化或快速进化具有新的和/或改进特性的多核苷酸、蛋白质、路径、细胞和/或生物体。这种改变序列的方法会产生,例如,单个核苷取代、多个核苷酸取代以及核酸序列区域的插入或缺失。
虽然在为了确保清楚地讨论的过程中进行区分和分类,但是应理解,这些技术通常不是相互排斥的。实际上,各种方法可以单独或联合、平行或依次地使用,以获得不同的序列变体。
本文所述的任何产生多样性的方法的结果可能是产生了一种或多种多核苷酸,可以从中选择或筛选编码具有或赋予所需特性的蛋白质的多核苷酸。在用一种或多种本文所述的方法或技术人员可获得的其它方法进行多样化后,可以从中选择具有所需活性或特性的多核苷酸,如改变的对草甘膦的Km、改变的对乙酰CoA的Km、使用备选的辅因子(如丙酰CoA)提高了k催化等。这包括,例如以自动或可自动化的形式,通过本领域的分析方法之一,确定任何可被检测的活性。例如,用质谱法,通过分析草甘膦转变为N-乙酰草甘膦,来检测特定活性升高的GAT同系物。或者,可以将使用本发明的核酸转化的细菌生长在含有浓度递增的草甘膦的琼脂上,或用草甘膦喷洒含有本发明核酸的转基因植物,这样可以测定增强的赋予草甘膦抗性的能力。根据实践者的判断力,可以连续或平行地评价各种相关(或者甚至是不相关)的特性。有关重组和选择除草剂抗性的其它详细描述可见于,例如,1999年8月12申请的“生产除草剂抗性农作物的DNA改组”(美国专利公开2002/0058249)。
对各种产生多样性的方法的描述,包括多基因改组和用于产生编码多个酶促结构域的被修饰核酸序列的方法,可以见于如下公开物及其中引用的参考文献:Soong,N.等人(2000)Nat.Genet.25(4):436-39;Stemmer等人(1999)Tumor Targeting 4:1-4;Ness等人(1999)Nature Biotech.17:893-896;Chang等人(1999)NatureBiotech.17:793-797;Minshull和Stemmer(1999)Current Opinionin Chemical Biology 3:284-290;Christians等人(1999)NatureBiotech.17:259-264;Crameri等人(1998)Nature 391:288-291;Crameri等人(1997)Nature Biotech.15:436-438;Zhang等人(1997)Proc.Natl.Acad.Sci.USA 94:4504-4509;Patten等人(1997)Current Opinion in Biotechnology 8:724-733;Crameri等人(1996)Nature Med.2:100-103;Crameri等人(1996)NatureBiotech.14:315-319;Gates等人(1996)J.Mol.Biol.255:373-386;Stemmer(1996),“Sexual PCR and Assembly PCR”,分子生物学百科全书(VCH出版社,纽约)第447-457页;Crameri和Stemmer(1995),Bio Techuigues 18:194-195;Stemmer等人(1995),Gene 164:49-53;Stemmer(1995),Science 270:1510;Stemmer(1995),Bio/Technolagy 13:549-553;Stemmer(1994)Nature 370:389-391;和Stemmer(1994),Proc.Natl.Acad.Sci.USA,91:10747-10751。
产生多样性的突变方法包括,如定点诱变(Ling等人(1997),“DNA诱变的方法:综述”,Anal Biochem.254(2):157-178;Dale等人(1996),“使用硫代磷酸酯法进行寡核苷酸指导的随机诱变”Methods Mol.Biol.57:369-374;Smith(1995),“体外诱变”Ann.Rev.Genet.19:423-462;Botstein & Shortle(1985),“体外诱变的策略和应用”Science 229:1193-1201;Carter(1986),“定点诱变”,Biochem J.237:1-7;和Kunkel(1987),“寡核苷酸指导的诱变效率”,Nucleic Acids & Molecular Biology(Eckstein,F.和Lilley,D.M.J.编辑,Springer Verlag,柏林);使用含有尿嘧啶的模板进行的诱变(Kunkel(1985)“不进行表型选择的快速和有效的位点特异性诱变”Proc.Natl.Acad.Sci.USA 82:488-492;Kunkel等(1987)“不进行表型选择的快速和有效的位点特异性诱变”Methods in Enzymol.154:367-382;和Bass等(1988)“具有新的DNA-结合特异性的突变Trp阻抑物”Science 242:240-245);寡核苷酸指导的诱变(Methods in Enzymol.100:468-500(1983);Methods in Euzymol.154:329-350(1987);Zoller&Smith(1982)“使用M13衍生的载体进行寡核苷酸指导的诱变:在DNA片段中产生点突变的有效和一般方法”Nucleic Acids Res.10:6487-6500;Zoller & Smith(1983)“克隆入M13载体中的DNA片段的寡核苷酸指导的诱变”Methods in Enzymol.100:468-500;和Zoller & Smith(1987)“寡核苷酸指导的诱变:使用两种寡核苷酸引物和单链DNA模板的简单方法”Methods in Enzymol.154:329-350);硫代磷酸酯修饰的DNA诱变(Taylor等人(1985)“在限制酶反应中使用硫代磷酸酯修饰的DNA制备裸DNA”Nucl.Acids Res.13:8749-8764;Taylor等(1985)“使用硫代磷酸酯修饰的DNA以高频率快速产生寡核苷酸指导的突变”Nucl.Acids Res.13:8765-8787;Nakamaye & Eckstein(1986)“硫代磷酸酯基团对限制性核酸内切酶Nci I切割的抑制及其在寡核苷酸指导的诱变中的应用”,Nucl.Acids Res.14:9679-9698;Sayers等人(1988)“基于硫代磷酸酯的寡核苷酸指导的诱变中的Y-T核酸外切酶”Nucl.Acids Res.16:791-802;和Sayers等人(1988)“通过在溴乙锭的存在下与限制性核酸内切酶反应对含有硫代磷酸酯的DNA进行链特异性切割”Nucl.Acids Res.16:803-814);使用带缺口的双链体DNA进行的诱变(Kramer等人(1984)“用于寡核苷酸指导的突变构建的带缺口的双链体DNA方法”Nucl.Acids Res.12:9441-9456;Kramer & Fritz(1987)Methods in Enzymol.“通过带缺口的双链体DNA进行的寡核苷酸指导的构建的突变”154:350-367;Kramer等人(1988)“对于寡核苷酸指导的突变构建在带缺口的双链体DNA方法中,改进的体外酶反应”Nucl.Acids Res.16:7207;和Fritz等人(1988),“寡核苷酸指导的突变构建:没有体外酶反应的带缺口的双链体DNA法”Nucl.Acids Res.16:6987-6999)。
其他的合适方法包括点错配修复(Kramer等人(1984)“点错配修复”Cell 38:879-887〕,使用修复缺陷的宿主细胞株进行的诱变(Carter等人(1985)“使用M13载体改进寡核苷酸定点诱变”Nucl.Acids Res.13:4431-4443;和Carter(1987)“使用M13载体进行改进的寡核苷酸指导的诱变”Methods in Enzymol 154:382-403),缺失诱变(Eghtedarzadeh & Henikoff(1986)“使用寡核苷酸产生大的缺失”Nucl.Acids Res.14:5115),限制性选择和限制性纯化(Wells等人(1986)“氢键形成对稳定枯草蛋白酶过渡态的重要性”Phil.Trans.R.Soc.Lond.A,317:415-423),通过总基因合成进行的诱变(Nambiar等人(1984)“编码核糖核酸酶S蛋白的基因的总合成和克隆”Science 223:1299-1301;Sakamar和Khorana(1988)“用于牛杆菌外片段鸟嘌呤核苷酸结合蛋白(转导素)的亚基的基因的总合成和表达”Nucl.Acids Res.14:6361-6372;Wells等人(1985),“表达盒诱变:在特定位点上产生多个突变的有效方法”Gene 34:315-323;和Grundstr_m等(1985),“通过微量‘鸟枪’基因合成进行的寡核苷酸指导的诱变”Nucl.Acids Res.13:3305-3316);双链断裂修复(Mandecki(1986);Arnold(1993)“用于异常环境的蛋白质工程”Current Opinion in Biotechnolgy4:450-455;和“大肠杆菌质粒中的寡核苷酸指导的双链断裂修复:一种定点诱变方法”Proc.Natl.Acad.Sci.USA,83:7177-7181)。上述方法的其他详细内容可见于Methods in Enzymology第154卷,其中还描述了用各种诱变方法有效控制打靶故障的问题。
关于各种产生多样性的方法的其他详细描述可见于如下的美国专利、PCT公开和EPO公开:Stemmer的美国专利5,605,793(1997年2月25日),“体外重组方法”;Stemmer等人的美国专利5,811,238(1998年9月22日),“通过反复选择和重组产生具有所需特性的多核苷酸的方法”;Stemmer等人的美国专利5,830,721(1998年11月3日),“采用随机片段化和重装配进行DNA诱变”;Stemmer等人的美国专利5,834,252(1998年11月10日),“未端互补聚合酶反应”;Minshull等人的美国专利5,837,458(1998年11月17日),“用于细胞和代谢工程的方法和组合物”;WO 95/22625,Stemmer和Crameri,“采用随机片段化和装配进行的诱变”;Stemmer和Lipschutz的WO 96/33207,“未端互补聚合酶链式反应”;Stemmer和Crameri的WO 97/20078,“通过反复选择和重组产生具有所需特性的多核苷酸的方法”;Minshull和Stemmer的WO 97/35966,“用于细胞和代谢工程的方法和组合物”;Punnonen等人的WO 99/41402,“遗传疫苗载体的靶标”;Punnonen等人的WO99/41383,“抗原库免疫”;Punnonen等人的WO 99/41369,“遗传疫苗载体工程”;Punnonen等人的WO 99/41368,“遗传疫苗的免疫调节性能的优化”;Stemmer和Crameri的EP 752008,“采用随机片段化和重装配进行的DNA诱变”;Stemmer的EP 0932670,“采用递推序列重组促进细胞DNA摄取”;Stemmer等的WO 99/23107,“采用病毒基因组改组进行的病毒嗜性和宿主范围的修饰”;Apt等人的WO99/21979,“人乳头瘤病毒载体”;del Cardayre等人的WO 98/31837,”“用递推序列重组推进全细胞和生物体”;Patten和Stemmer的WO 98/27230,“用于多肽工程的方法和组合物”;Stemmer等人的WO 98/13487,“采用递推序列改组和选择优化基因治疗的方法”,WO 00/00632,“产生高度多样化文库的方法”,WO 00/09679,“获得体外重组多核苷酸序列库和所产生的序列的方法”;Arnold等人的WO 98/42832,“使用随机的或限定的引物重组多核苷酸序列”;Arnold等人的WO 99/29902,“产生多核苷酸和多肽序列的方法”;Vind的WO 98/41653,“构建DNA文库的体外方法”;Borchert等WO 98/41622,“使用DNA改组构建文库的方法”;Pati和Zarling的WO 98/42727,“用同源重组进行序列改变”;Patten等人的WO 00/18906,“密码子改变的基因改组”;Cardayre等人的WO 00/04190,“采用递推序列重组的全细胞和生物体进化”;Crameri等人的WO 00/42561,“寡核苷酸介导的核酸重组”;Selifonov和Stemmer的WO 00/42559,“用于进化模拟中的繁殖数据结构的方法”;Salifonov等人的WO 00/42560,“制备具有所需特征的特征链串、多核苷酸和多肽的方法”;Welch等人的WO 01/23401,“将密码子改变的寡核苷酸合成法用于合成改组”;以及Affholter的PCT/US01/06775,“单链核酸模板介导的重组和核酸片段分离”。
某些美国申请还提供了关于产生多样性的方法的更详细的描述,包括:Patten等人在1999年9月28日申请的“密码子改变的基因的改组”(USSN 09/407800);del Cardayre等人在1998年7月15日(USSN 09/166188)和1999年7月15日(USSN 09/354922)申请的“采用递推序列重组进化全细胞和生物体”;Crameri等人在1999年9月28日申请的“寡核苷酸介导的核酸重组”(USSN 09/408392);Crameri等人在2000年1月18日申请的“寡核苷酸介导的核酸重组”(PCT/US00/01203);Welch等人在1999年9月28日申请的“将基于密码子的寡核苷酸合成用于合成性改组”(USSN 09/408393);Selifonov等人在2000年1月18日申请的“具有所需特征的制备特字符串多核苷酸和多肽的方法”(PCT/US00/01202);Selifonov等人在200年7月18日申请的“制备具有所需特征的字符串、多核苷酸和多肽的方法”(USSN 09/618579);Selifonov和Stemmer在2000年1月18日申请的“用于进化模拟中的繁殖数据结构的方法”(PCT/US00/01138);Affholter的“单链核酸模板介导的重组和核酸片段分离”(USSN 60/186482,2000年3月2日申请)。
简而言之,几类不同的常规序列修饰方法,如突变、重组等,可应用于本发明,并且在上述参考文献中列举。也就是说,在序列共连接之前或在共连接步骤之后,用任何一种上述方案改变核酸序列的组成,产生修饰的基因融合构建体。下面在本发明文中例举了一些不同类型的优选形式,用于产生多样化,包括,例如某些基于多样化产生形式的重组。
用上述参考文献中讨论的各种技术,进行核酸的体外重组,包括,例如DNA酶消化要被重组的核酸,然后连接和/或PCR重组该核酸。例如,可以使用有性PCR诱变,其中DNA分子被随机(或假随机,或甚至非随机)断裂,然后基于具有不同但相关DNA序列的DNA分子之间的相似性进行体外重组,然后通过在聚合酶链式反应中延伸来固定交换体。此过程和多种该过程的变化形式被描述在上述几个参考文献中,例如Stemmer(1994)Proc.Natl.Acad.Sci.USA 91:10747-10751。
类似地,可以在体内递推重组核酸,例如使细胞内的核酸之间发生重组。上面提到的参考文献中列出了许多这种体内重组形式。这种形式通常任选地在感兴趣的核酸之间直接重组,或在含有感兴趣的核酸的载体、病毒、质粒等之间进行重组,以及其它形式。有关该过程的详细描述可见于上述参考文献。
也可以使用全基因组重组法,其中细胞或其它生物的全基因组被重组,任选地包括掺入带有所需文库成分(如对应于本发明的路径的基因)的基因组重组混合物。这些方法有许多应用,包括靶基因特性不清楚的那些应用。关于这种方法的详细描述可见于,例如del Cardayre等人的WO 98/31837,“用递推序列重组推进全细胞和生物体”,和例如del Cardayre等人的WO0/004190,标题为“用递推序列重组推进全细胞和生物体”。因此,可以单独或联合使用这些用于重组、递推重组以及全基因组重组的方法和技术中的任何一种,来生产本发明的经修饰的核酸序列和/或经修饰的基因融合构建体。
也可以采用合成重组法,其中在PCR或连接反应中,合成并重组了对应于感兴趣靶子的寡核苷酸,包括与一个以上的亲代核酸相对应的寡核苷酸,因此生成新的重组核酸。可以用标准的核苷酸加成法生产寡核苷酸,或者可以例如通过三联核苷酸合成法来生产寡核苷酸。有关这种方法的详细描述可见于上述参考文献,包括,例如Crameri等人的WO 00/42561,“寡核苷酸介导的核酸重组”;Welch等人的WO01/2340,“将基于密码子的寡核苷酸合成用于合成性改组”;Selifonov等人的WO 00/42560,“制备具有所需特征的字符串、多核苷酸和多肽的方法”;Selifonov和Stemmer的WO 00/42559,“用于进化模拟中的繁殖数据结构的方法”。
可采用计算机芯片内(in silico)重组方法,其中在计算机中使用遗传算法,重组对应于同源(乃至非同源)核酸的序列链。通过合成对应于重组序列的核酸,如与寡核苷酸合成/基因重装配技术协同,将生成的重组序列链任选地转化成核酸。这种方法可产生随机的、部分随机的或所设计的变体。关于计算机芯片内重组的许多细节,包括遗传算法、遗传操纵子在计算机系统中的应用、结合与相应的核酸(和/或蛋白质)的产生相结合、以及与所设计的核酸和/或蛋白质(如基于横跨位点的选择)以及设计的、假随机的或随机的组合相结合的方法,被描述在Selifonov等人的WO 00/42560,“制备具有所需特性的字符串、多核苷酸和多肽的方法”;Selifonov和Stemmer的WO00/42559,“用于进化模拟中增加数据结构的方法”。关于计算机芯片内重组方法的更多详细描述可见于这些申请。通常可将这种方法用于本发明,以在计算机芯片内将编码参与各种代谢途径(如类胡萝卜素生物合成途径、外蛋白(ectoine)生物合成途径、聚羟基链烷酸酯生物合成途径、芳族聚酮化合物生物合成途径等)的蛋白质的核酸序列和/或基因融合构建体进行重组,和/或产生相应的核酸或蛋白质。
可类似地采用评估天然多样性的许多方法,如通过使不同的核酸或核酸片段与单链模板杂交,然后经过聚合和/或连接,重新生成全长序列,然后任选地降解模板,回收所生成的修饰核酸。在使用单链模板的一种方法中,使来源于基因组文库的片段群与对应于相反链的部分的或通常约全长的ssDNA或RNA退火。然后,使用核酸酶除去非杂交片段末端,聚合填满这些片段之间的空隙,接着进行单链连接,从而介导由此群体装配得到复杂的嵌合基因。通过消化(例如如果含有RNA或尿嘧啶)、在变性条件下的磁力分离(以有益于这种分离方式进行标记)和其它可利用的分离/纯化方法,去除亲本多核苷酸链。或者,任选地将亲本链与嵌合链共纯化,并在随后的筛选和加工步骤中除去。关于这种方法的其他详细描述可见于Affholter的WO 01/64864,“单链核酸模板介导的重组和核酸片段分离”。
在另一种方法中,将单链分子转化成双链DNA(dsDNA),然后通过配体介导的结合使该dsDNA分子结合到固相支持物上。在将未结合的DNA分离后,从支持物上释放所选择的DNA分子,然后将其导入合适的宿主细胞中,产生富含与该探针杂交的序列的文库。以这种方式生成的文库为采用在此描述的任一种方法进一步多样化提供了所需的底物。
前述的常规重组形式的任何一种都可以重复进行(如一轮或多轮突变/重组,或者其它产生多样性的方法,任选地采用一种或多种选择方法),产生一组更加多样化的重组核酸。
还提出了用多核苷酸链终止法进行诱变(参见,例如Short的美国专利5,965,408,“中断合成重组DNA的方法”,以及上面的文献),并且可以将该方法应用于本发明。在该方法中,在存在或缺乏该基因的特异性引物的情况下,将对应于具有序列相似性区域的一个或多个基因的双链DNA混合并变性。然后使这些单链多核苷酸退火,并在聚合酶和链终止剂(如,紫外光、γ射线或X-射线;溴化乙锭或其它嵌入剂;DNA结合蛋白,如单链结合蛋白、转录激活因子或者组蛋白;多环芳烃;三价铬或三价铬盐;或者由快速热循环介导的简易聚合;等等)的存在下进行温育,得到部分双链体分子。然后使这些例如含有部分延伸链的部分双链体分子变性,并在随后的复制和部分复制循环中再退火,产生共有不同程度的序列相似性的多核苷酸,与起始的DNA分子群相比,这些多核苷酸被多样化。任选地,在该方法的一个或多个阶段中,将产物或者部分产物的集合扩增。通过上述的链式终止法制备的多核苷酸是用于任何其它所述的重组方式的合适底物。
还可采用Ostermeier等人(1999)在“与DNA同源性无关的杂交酶的组合方法”Nature Biotech,17:1205中所述的“产生杂交酶的渐进截短”(“ITCHY”)中命名的重组方法,在核酸或核酸群中产生多样性。可采用这种方法产生变体的起始文库,该变体任选地作为一种或多种体外或体内重组方法的底物。还可参见Ostermeier等人(1999),“采用渐进截短进行的组合蛋白质工程”,Proc.Natl.Acad.Sci.USA,96:3562-67;Ostermeier等人(1999),“在新颖的生物催化剂的工程中以渐进截短作为策略”,Biol.Med.Chem,7:2139-44。
可有利地采用导致单个核苷酸或者邻接或非邻接核苷酸组改变的突变方法,将核苷酸多样性导入本发明的核酸序列和/或基因融合构建体中。许多诱变方法可见于上述参考文献;关于诱变方法的其他详细描述可见在下文,它们也被应用于本发明。
例如,可采用易错PCR产生核酸变体。采用这种技术,在DNA聚合酶复制保真性低的条件下进行PCR,使得在PCR产物的整个长度获得高比例的点突变。这些技术的例子可见于上述参考文献,例如Leung等人(1989),Technique 1:11-15;和Caldwell等人(1992),PCR Methods Applic.,2:28-33。类似地,在涉及从小DNA片段的混合物装配PCR产物的过程中,可采用装配PCR。在同一反应混合物中,同时发生大量不同的PCR反应,一个反应的产物引发另一反应的产物。
可采用寡核苷酸指导的诱变,在感兴趣的核酸序列中导入位点特异性突变。这种技术的例子可见于上述参考文献,以及如Reidhaar-Olson等人(1988),Science 241:53-57。类似地,在使用与天然序列不同的合成寡核苷酸表达盒替换双链DNA分子的小区域的过程中,可采用表达盒诱变。寡核苷酸可含有如完全和/或部分随机化的天然序列。
递推集团诱变(recursive ensemble mutagenesis)是一种将蛋白质诱变的算法用于产生表型相关的突变体的多样化群体的方法,该群体中的成员在氨基酸序列上不同。这种方法使用反馈机制来监测数轮组合型表达盒的诱变。这种方法的例子可见于Arkin & Youvan(1992),Proc.Natl.Acad.Sci.USA 89:7811-7815。
采用指数集团诱变法,用于产生含有高比例的独特和功能性的突变体的组合文库。感兴趣的序列中的少量残基被平行地随机化,以鉴定在各改变位置上产生功能性蛋白质的氨基酸。这类方法的例子可见于Delegrave & Youvan(1993),Biotech.Res.11:1548-1552。
可采用体内诱变,通过在携带一个或多个DNA修复途径中的突变的大肠杆菌菌株中繁殖DNA,在感兴趣的任何克隆DNA中产生随机突变。这些“突变子”株比野生型亲本具有较高的随机突变率。在这些菌株之一中繁殖DNA,最终将在该DNA中产生随机突变。这些方法在上述参考文献中有描述。
在如细菌、真菌、动物或植物基因组的一种基因组中产生多样性的其它方法可与上述和/或所参考的方法一起使用。例如,除了上述方法外,还提出了生产适用于转化到各种物种中的核酸多聚体的技术(例如,可参见Schellenberger,美国专利5,756,316和上述参考文献)。用这类由相互之间不同的基因(如从天然的多样性得到的,或者通过采用定点诱变、易错PCR获得的,通过诱变细菌菌株传代获得的等)组成的多聚体转化合适的宿主,提供用于DNA多样化的核酸多样性的来源,如通过上述体内重组方法。
或者,将多种共有部分序列相似区域的单体多核苷酸转化入宿主物种中,由宿主细胞在体内进行重组。接下来的多轮细胞分裂可用来产生文库,文库的成员包括单一的同源群体,或者单体多核苷酸的集合。或者,可采用标准的技术,如PCR和/或克隆,来回收单体核酸,然后以任何一种重组方式,包括上述递推重组方式进行重组。
已描述了产生多物种表达文库的方法(除了上述参考文献以外,还参见Peterson等人(1998),美国专利5,783,431,“产生和筛选新颖代谢途径的方法”;和Thompson等人(1998),美国专利5,824,485,“产生和筛选新颖代途径的方法”),还提出了它们在鉴定感兴趣的蛋白质活性方面的用途〔除了上述文献以外,还可参见Short(1999)的美国专利5,9586,72,“具有从非培养的微生物DNA的克隆的蛋白质活性筛选”〕。通常,多物种表达文库包括含有多种物种或菌株的、在表达盒中可操作地连接到适当的调节序列的cDNA或基因组序列的文库。该cDNA和/或基因组序列任选地随机连接,以进一步提高多样性。载体可以是适用于在一种以上宿主生物体中,如在细菌物种、真核细胞中转化和表达的穿梭载体。在某些情形下,通过预先选择编码感兴趣的蛋白质的序列,或者与感兴趣的核酸杂交的序列,使得该文库被偏倚。可提供这样的文库用作本文所述任一方法的底物。
已广泛采用上述方法来增加核酸和/或编码的蛋白质多样性。但是,在许多情况下,并不是所有的多样性都是有用的,如功能方面,多样性仅仅有助于增加变体的背景,必须对这些变体进行筛选或选择来确定少数有利的变体。在一些应用中,需要在多样化之前预先选择或预先筛选文库(如扩增的文库、基因组文库、cDNA文库、归一化文库等)或其它底物核酸。例如,在抗体工程中,在采用上述任一种方法进行操作前利用体内重组活动,有可能使产生多样性的方法偏向具有功能性抗原结合位点的抗体。例如,可在采用本文所述的任一种方法进行多样化之前,先扩增来源于B细胞cDNA文库的重组CDR,然后装配到构架区域中〔如Jirholt等人(1998),“利用序列空间:在体内将形成的互补性决定簇区域改组到主要构架中”Gene 215:471〕。
文库偏向于编码具有所需酶活性的蛋白质的核酸。例如,在从具有特殊活性的文库中鉴定到一个克隆后,可采用任何已知的用于导入DNA改变的方法来诱变该克隆。然后根据所需的活性筛选含有诱变同系物的文库,该活性可能与初始的特殊活性相同或者不同。这种方法的一个例子是Short(1999)在美国专利5,939,250,“通过诱变产生具有所需活性的酶”中提出的。可采用本领域已知的任何方法鉴定所需的活性。例如WO 99/10539提出,可通过将基因文库的提取物与从代谢旺盛的细胞获得的成分相组合,并且鉴定具有期望活性的组合。还提出(如WO 98/58085),可通过将生物活性底物插入文库的样品中,然后使用荧光分析仪,如流式细胞仪装置、CCD、荧光计或光谱仪,并且检测对应于具有期望活性的产物的生物活性荧光,从而鉴定出具有期望活性的克隆。
文库还可偏向于具有特殊特性的核酸,例如与选择的核酸探针杂交的核酸。例如,WO 99/10539提出,可以从基因组DNA序列中鉴定出编码期望活性(酶活性,例如:脂肪酶、酯酶、蛋白酶、糖苷酶、糖基转移酶、磷酸酶、激酶、加氧酶、过氧化物酶、水解酶、水合酶、腈水解酶、转氨酶、酰胺酶或酰基转移酶)的多核苷酸。特别地,来自基因组DNA群体的单链DNA分子与配体偶联的探针杂交。基因组DNA来源于培养的或非培养的微生物,或者来源于环境样品中。或者,基因组DNA可来源于多细胞生物或其组织。可以用事先从捕获培养基中释放的或未释放的用于捕获的杂交探针直接进行第二链合成,或者可采用本领域已知的各种其它策略进行。或者,不需进一步克隆,将分离的单链基因组DNA群体片段化,直接用于例如基于重组的方法,如上所述,该方法使用单链模板。
在Short的WO 00/46344“基因疫苗和酶的非随机性产生”中描述了产生核酸和多肽的“非随机性”方法。这些方法,包括所提出的非随机性多核苷酸重新装配和位点饱和性诱变方法都可用于本发明。采用掺杂或简并寡核苷酸进行的随机或半随机诱变也有描述,如Arkin和Youvan(1992),“优化核苷酸混合物,以编码用于半随机诱变氨基酸的特殊亚组”,Biotechnology 10:297-300;Reidhaar-Olson等人(1991),“使用寡核苷酸盒对蛋白质序列进行随机诱变”,MethodsEnzymol.208:564-86;Lim和Sauer(1991),“内部包装相互作用在决定蛋白质的结构和稳定性中的作用”J.Mol.Biol.219:359-76;Breyer和Sauer(1989),“单克隆抗体51F与λ阻抑物的良好特异性结合的突变分析”J.Biol.Chem.264:13355-60;和“初排(walk-through)诱变”(Crea,R,美国专利5,830,650和5,798,208,和欧洲专利0527809 B1)。
不难理解,上述适用于在多样化前富集文库的技术也可用于筛选由产生多样性方法获得的产物或者产物文库。可以递推地或联合地实施上述方法的任何一种,以改变核酸,如GAT编码多核苷酸。
用于诱变、文库构建和其它产生多样性的方法的试剂盒也可购买得到。例如,可从Stratagene(如QuickChangeTM定点诱变试剂盒;和ChameleonTM双链、定点诱变试剂盒);Bio/Can Scientific、Bio-Rad(例如,使用上述Kunkel方法);Boehringer Mannheim公司;Clonetech实验室;DNA Technologies;Epicentre Technologies(如5引物3引物试剂盒);Genpak公司;Lemargo公司;LifeTechnologies(Gibco BRL);New England Biolabs;PharmaciaBiotech;Promega公司;Quantum Biotechnologies;AmershamInternational plc(例如,采用上述Eckstein法)和AnglianBiotechnology有限公司(例如,采用上述Carter/Winter法)。
上述参考文献提供了许多突变方式,包括重组、递推重组、递推突变和重组或者具有其它诱变形式的重组,以及这些方式的许多改变形式。不管采用哪种产生多样性的方式,都可以重组本发明的核酸(相互之间,或者与相关的(乃至无关的)序列之间),来产生用于本发明的基因融合构建体和修饰的基因融合构建体的重组核酸的不同组合,包括例如同源核酸以及相应的多肽。
上述许多产生修饰的多核苷酸、序列的方法产生了亲本序列的大量多样性变体。在本发明的一些优选实施例中,采用经改动的技术(如某些形式的改组),来产生变体的文库,然后筛选此文库中的修饰的多核苷酸或者修饰的多核苷酸的集合,这些多核苷酸编码一些期望的功能特性,如改进的GAT活性。可用于筛选的示例性酶活性的例子包括催化速率(通常以动力学常数如k催化和KM为特征)、底物特异性和对底物、产物或其它分子(如抑制剂或激活剂)的激活或抑制的易感性。
选择期望的酶活性的一个例子要求使宿主细胞生长在抑制不能充分表达感兴趣的酶活性如GAT活性的细胞生长和/或生存的条件下。采用这种选择方法,可以不用考虑编码期望酶活性的修饰核酸以外的所有被修饰的核酸。例如,在本发明的一些实施方案中,将宿主细胞维持在缺乏足够水平的草甘膦时抑制细胞生长或存活的条件下。在这些条件下,仅仅含有修饰核酸的宿主细胞能生存和生长,该修饰核酸编码能催化产生足够水平的产物的酶活性。本发明的某些实施方案采用在浓度递增的草甘膦或草甘膦类似物中进行多轮筛选。
在本发明的某些实施方案中,采用质谱来检测草甘膦、草甘膦类似物或代谢物的乙酰化。在以下实施例中更加详细地描述质谱应用。
为了方便以及获得高通量,常常需要在微生物,如细菌,如大肠杆菌中筛选/选择期望的修饰核酸。另一方面,在某些情况下,在植物细胞或植物中进行筛选是优选的,其最终的目的是产生用于在植物系统中表达的修饰核酸。
在本发明的一些优选的实施方案中,通过对表达不同的修饰核酸的宿主细胞集合进行筛选,单独地或者作为基因融合构建体的一部分,来增加通量。可对显示出显著活性的任何集合进行反褶积,以确定表达期望活性的单个克隆。
本领域技术人员将认识到,相关分析、筛选或选择方法将根据所需宿主生物和本领域知晓的其他参数而改变。通常,采用可以以高通量形式实施的分析是有利的。
在高通量分析中,有可能在一天内就筛选到几千种不同的变体。例如,使用微量滴定板的各个孔来进行分离分析,或者,如果要观察浓度或培育时间的效果,每5-10个孔可以测试一种变体。
除了液体方法以外,如上所述,有可能简单地使细胞生长在培养基平板上,选择期望的酶功能或代谢功能。这种方法提供了一种简单和高通量的筛选方法。
也已经开发了许多众所周知的机器人系统,用于分析系统中的溶液相化学。这些系统包括自动工作站,如Takeda Chemical Industries有限公司(大阪,日本)开发的自动合成装置和许多使用机械臂的机器人系统(Zymate II,Zymark公司,Hopkinton,MA;Orca,Hewlett-Packard,Palo Alto,加利福尼亚州),该系统模拟科学家进行的人工合成操作。上述任一设备都适合在本发明中应用。对这些装置进行改动(如果有的话),以使它们如本文所述参照集成系统进行操作,这些改动的性质和实施对于相关领域的技术人员来说是显而易见的。
高通量筛选系统可以购买得到(例如,参见Zymark公司,Hopkinton,MA;Air Technical Industries,Mentor,OH;BeckmannInstruments公司。Fullerton,加利福尼亚州;Precision Systems公司,Natick,MA等)。这些系统通常自动操作整个过程,包括所有样品和试剂的吸取、液体分散、定时培育和在适用于该分析的检测器中对微滴定板进行最后读数。这些可配置的系统提供了高通量和快速起始,以及高度的灵活性和用户化。
这类系统的制造商为各种高通量设备提供了详细的方案。因此,例如,Zymark公司提供了描述用于检测基因转录、配体结合等调节的筛选系统的技术手册。Caliper Technologies(Mountain View,加利福尼亚州)也开发了试剂操作的微流体方法。
在本文所述的实施方案中,还任选地用照相机或其它记录设备(如光电二极管和数字存储设备)观察(以及任选地记录)光学图像,例如通过使图像数字化和/或在计算机上存储和分析该图像。可使用各种可购买得到的外围设备和软件,使录像或光学图像数字化,并进行存储和分析,如使用PC(Intel×86或者与奔腾芯片相容的DOSTM、OSTMWINDOWSTM、WINDOWS NTTM或者WINDOWS 95TM为基础的机器)、MACINTOSHTM或者UNIX为基础的(如SUNTM工作站)计算机。
一个常规系统将来自分析设备的光线传送给本领域常用的冷却的电荷耦合的装置(CCD)的照相机。CCD照相机包括图像元素的一个阵列(像素)。样品的光线在CCD上成像。对应于该样品的各区域的具体的像素(如在生物聚合物的阵列上的单个杂交位点)被取样,以获得各位置上的光强度读数。平行加工多个像素,以增加速度。不难采用本发明的装置和方法来观察任何样品,例如通过荧光或暗视野显微镜技术来观察样。
其它多核苷酸组合物
本发明还包括一种组合物,其含有两种或多种本发明的多核苷酸(如,作为重组底物)。这种组合物可以包含重组核酸文库,该文库含有至少2种、3种、5种、10种、20种或50种或更多种的多核苷酸。这些多核苷酸任选地被克隆入表达载体,提供表达文库。
本发明还包括用限制性内切酶、RNA酶或DNA酶消化一种或多种本发明的多核苷酸而生成的组合物(例如,如以上述某些重组形式进行);以及通过机械方法(如超声、涡旋等)断裂或剪切一个或多个本发明的多肽而产生的组合物,机械方法也可被用于提供在上述方法中用于重组的底物。类似地,含有若干组与本发明的一种以上的核酸相对应的寡核苷酸的组合物可以被用作重组底物,并且是本发明的一个特点。为了简便,这些断裂的、剪切的、或寡核苷酸合成的混合物被称为片段化的核酸组。
本发明还包括在核糖核苷酸或脱氧核糖核苷三磷酸和核酸聚合酶存在时通过培养一种或多种片段化的核酸组而制得的组合物。所产生的组合物形成了可用于上述许多重组模式的重组混合物。核酸聚合酶可以是RNA聚合酶、DNA聚合酶或RNA-引导的DNA聚合酶(即“反转录酶”);聚合酶可以是,例如,热稳定的DNA聚合酶(如VENT、TAQ等)。
集成系统
本发明提供了计算机、计算机可读介质和含有与本文的多肽和核酸的序列信息相对应的字符串的集成系统,所述序列例如包括这里列出的那些序列及其各种沉默取代和保守取代。
例如,本领域已知的各种方法和遗传算法(GA)可用来测定不同字符串之间的同源性或相似性,或者可用来完成其它所需的功能,比如控制输出文件、提供用于呈现包括序列在内的信息的基础。其例子包括上述的BLAST。
因此,在本文的集成系统中,可以检测并识别具有各种严紧性和长度的不同类型的同源性和相似性。例如,为了比较分析生物多聚物的序列、在字处理中进行拼写检查以及为从各种数据库中获得数据,已经设计了许多确定同源性的方法。随着对天然多核苷酸中4个基础核碱基之间双螺旋互补配对反应的了解,模拟互补同源多核苷酸链退火的模型,也可用作序列比对的基础,或典型地针对与本文的序列相对应的字符串进行的其他操作(如字处理操作、构建含有序列或亚序列字符串的图表、输出表格等)的基础。用于计算序列相似性的具有GA的软件包的例子是BLAST,通过输入与本文序列相对应的字符串,BLAST可适用于本发明。
类似地,通过输入与本发明GAT同系物(核酸或蛋白质,或这两者)相对应的字符串,可以使标准桌面应用如字处理软件(例如MicrosoftWordTM或Corel WordPerfectTM)和数据库软件(例如电子制表软件如Microsoft ExcelTM、Corel Quattro ProTM,或数据库程序如MicrosoftAccessTM或ParadoxTM)适用于本发明。例如,此集成系统可以包括上述具有合适字符串信息的软件,例如和用户界面结合使用(如Windows、Macintosh或LINUX系统等标准操作系统中的GUI),以处理字符串。值得注意的是,也可以将专门的序列比对程序如BLAST合并入本发明的系统用于核酸或蛋白质(或相应的字符串)的序列的比对。
用于本发明分析的集成系统通常包括数字计算机、装有序列对比GA软件以及进入包含本文任何序列的软件系统的数据组。计算机可以是,例如,PC(Intel×86或者与奔腾芯片相容的以DOSTM、OSTMWINDOWSTM、WINDOWS NTTM或者WINDOWS 95TM为基础的机器)、MACINTOSHTM Power PC或者以UNIX(如SUNTM工作站)为基础的机器或技术人员已知的其它商品化普通计算机。用于序列对比或其它处理序列的软件是可以获得的,或者可以由技术人员采用Visual basic、Fortan、Basic、Java等标准编程语言进行编写。
任何控制器或计算机任选地包括一监测器,它通常是阴极射线管(“CRT”)显示器、平板显示器(如活性基质液晶显示器、液晶显示器)或其它设备。计算机线路通常被置于含有许多集成电路芯片如微处理器、储存器、接口电路等的机箱中。机箱也任选地包括硬盘驱动器、光盘驱动器、高容量可移动驱动器如可写CD-ROM和其它普通的外围元件。键盘或鼠标等输入设备可选地提供了用了由用户输入和用户选择作为比较或在有关计算机系统中进行其它处理的序列。
计算机通常包括合适的接收用户指令的软件,它要么以用户输入一组参数字段的形式,如在GUI中,要么以预排程序的指令的形式,如为各种不同的特殊操作预排程序。然后,软件将这些指令转换成合适的语言,用于指导数据流方向的操作并让控制器实施所需的操作。
软件还可以包括控制核酸合成(如基于本文序列或序列的对比)的输出元件或其它在序列对比后进行的操作或其它采用本文序列相应的字符串进行的操作。因此,核酸合成设备可以是本文的一种或多种集成系统的一个组件。
其它方面,本发明提供了包含本发明的方法、组合物、系统和设备的试剂盒。本发明的试剂盒任选地含有以下一种或多种:(1)本发明所述的设备、系统、系统组件或设备组件;(2)用于实施本发明所述方法,和/或操作本发明的装置或装置组件,和/或使用本发明组合物的说明书;(3)一种或多种GAT组合物或成分;(4)装载各组分或组合物的容器;以及(5)包装材料。
在另一方面,本发明提供了任何本文的装置、装置组件、组合物或试剂盒,用于实施任何本文所述的方法或试验的用途,和/或任何装置或试剂盒在实施任何本文所述的分析或方法中的用途。
宿主细胞和生物
宿主细胞可以是真核的,例如真核细胞、植物细胞、动物细胞、原生质体或者组织培养细胞。宿主细胞任选地含有多种细胞,例如生物体。或者,宿主细胞可以是原核的,包括但不限于细菌(即革兰阳性菌、紫色细菌、绿硫菌、绿色非硫菌、蓝细菌、螺旋体、栖热袍菌(thermatogale)、黄杆菌和类菌体)和古细菌(即Korarchaeota、热变形杆菌(Thermoproteus)、热网菌属(Pyrodictium)、热球菌属(Thermococcales)、产甲烷菌、古生球菌属(Archaeoglobus)和极端嗜盐菌)。
含有GAT核酸和/或表达本发明GAT多肽的转基因植物或植物细胞是本发明的一个特征。实际上,可以用植物分子生物学领域的技术人员已知的各种方法进行植物细胞和原生质体的转化,这些方法包括但不限于本文所描述的方法。通常可以参见Wu和Grossman编写的Methods in Enzymology,第153卷(Recombinant DNA第D部分)1987,学术出版社;以及Weising等人,Ann.Rev.Genet.22:421-477(1988),在此引入作为参考。例如,将DNA构建体直接导入植物细胞的基因组DNA中,采用如电穿孔、PEG介导的转化、粒子轰击、硅纤维呈递或者显微注射植物细胞原生质体或胚发生愈伤组织的方法。参见,例如Tome等人(1995)“通过显微投影轰击将DNA转移到完整的植物细胞中”,在Gamborg和Philips编辑的Plant cell,Tissue andOrgan Culture,Fundamental Methods(Springer-Verlag,柏林),第197-213页中。用于转化各种宿主细胞的其他方法被公开在Klein等人(1992)“通过粒子轰击进行的微生物、植物和动物的转化”Bio/Technol.10(3):286-291。
用聚乙二醇沉淀来导入DNA构建体被描述在Paszkowski等人(1984)EMBOJ.3:2717-2722中。电穿孔技术被描述在Fromm等人(1985)Proc.Natl.Acad.Sci.82:5824中。弹道转化技术描述在Klein等人(1987)Nature 327:70-73中。
此外,可以将DNA构建体与适当的T-DNA侧翼区组合,并且导入到常规的根癌农杆菌宿主载体中。当用细菌感染细胞时,根癌农杆菌宿主毒力功能将引导构建体与相邻标记插入到植物细胞DNA中。参加美国专利5,591,616。
已经在科技文献中充分地描述了根癌农杆菌介导的转化技术。参见,例如Horsch等人(1984)Science 233:496-498,以及Fraley等人(1983)Proc.Natl.Acad.Sci.80:4803.例如,在美国专利5,550,318和5,981,840中描述了用农杆菌转化玉米。
其他的转化方法包括(1)毛根农杆菌介导的转化(参见,例如Lichtenstein和Fuller的Genetic Engineering,第6卷,PWJ Rigby编辑,伦敦,学院出版社,1987;Lichtenstein,C.P.和Draper,J,DNA Cloning,第II卷,D.M.Glover编辑,牛津,IRI出版社,1985;WO 88/02405描述了毛根农杆菌菌株A4及其Ri质粒以及根癌农杆菌载体pARC8或pArC16的用途);(2)脂质体介导的DNA摄取(参见,例如Freeman等人(1984)Plant Cell Physiol.25:1353;(3)旋涡方法(参见,例如Kindle(1990)Proc.Natl.Acad.Sci.USA 87:1228。
还可以如Zhou等人(1983)Methods in Enzymology 101:433;D.Hess(1987)Intern Rev.Cytol.107:367和Luo等人(1988)Plant Mol.Biol.Reporter 6:165中所描述,通过将DNA转移到花粉中,将DNA导入到植物中。可以如Pena等人(1987)Nature 325:274中所描述,将DNA注射到植物的繁殖器官中,来获得对多肽编码核酸的编码。如Neuhaus等人(1987)Theor.Appl.Genet.75:30以及Benbrook等人(1986)在Proceedings Bio Expo 1986,Butterworth,Stoneham,Mass.第27-54页中描述,将DNA直接注射到未成熟的胚胎的细胞中,再水合干燥胚胎。
动物和低等真核生物(例如酵母)的宿主细胞能够转化或者被恢复为能够通过各种方法来转化。有多种将DNA导入动物细胞中的公知方法。这些方法包括:磷酸钙沉淀;受体细胞与含有DNA的细菌原生质体的融合;用含有DNA的脂质体处理受体细胞;DEAE葡聚糖;电穿孔;生物导弹轰击法(biolistics);以及将DNA直接显微注射到细胞中。用本领域熟知的方法来培养被转化的细胞。参见Kuchler,R.J.(1977)Biochemical Methods in Cell Culture and Virology(Dowden,Hutchinson和Ross公司)。当用于本文时,术语“转化”是指通过引入核酸序列,如“异源”或“外源”核酸序列,来改变宿主植株的基因型。异源核酸序列不一定来自不同的来源,但有些时候来自其将导入的细胞之外。
除了Berger、Ausubel和Sambrook之外,用于植物细胞克隆、培养和再生的一般性参考文献包括:Jones编辑(1995)Plant GeneTransfer and Expression Protocols-Methods in MolecularBiology第49卷(Humana出版社,Towata,新泽西州);Payne等人(1992),Plant Cell and Tissue Culture in Liquid Systems(John Wiley & Sons公司,纽约,纽约州)(″Payne″);和Gamborg和Philips编辑(1995),Plant Cell,Tissue and Organ Culture;Fundamental Methods/Springe Lab Manual,(Springer-Verlag,柏林)(“Gamborg”)。在以下文献中描述了各种细胞培养基:Atlas和Parks(编辑)Handbook of Microbiological Media(CRC出版社,Boca Raton,佛罗里达州)(“Atlas”)。植物细胞培养的其它信息可从商业文献中获得,如来自Sigma-Aldrich公司(圣路易斯,密苏里州)的Life Science Research Cell Culture Catalogue(1998)(Sigma-LSRCCC),以及得自Sigma-Aldrich公司(圣路易斯,密苏里州)的Plant Culture Catalogue增刊(1997)(Sigm-PCCS)。其它有关植物细胞培养的细节可以在Croy编辑(1993)Plant Molecular Biology(Bios Scientific出版社,牛津,英国)中找到。
在本发明的实施方案中,制备了适合植物细胞转化的含有一种或多种GAT多核苷酸的重组载体。编码期望的GAT多肽的DNA序列可方便地用来构建可以被引入期望的植物中的重组表达盒,所述DNA序列选自SEQ ID NO:516、517、518、519、520、521、522、523、524、525、526、527、528、529、530、531、532、533、534、535、536、537、538、539、540、541、542、543、544、545、546、547、548、549、550、551、552、553、554、555、556、557、558、559、560、561、562、563、564、565、566、567、620、622、624、626、628、630、632、634、636、638、640、642、644、646、648、650、652、654、656、658、660、662、664、666、668、670、672、674、676、678、680、682、684、686、688、690、692、694、696、698、700、702、704、706、708、710、712、714、716、718、720、722、724、726、728、730、732、734、736、738、740、742、744、746、748、750、752、754、756、758、760、762、764、768、770、772、774、776、778、780、782、784、786、788、790、792、794、796、798、800、802、804、806、808、810、812、814、816、818、820、822、824、832、834、836、838、840、842、844、846、848、850、852、854、856、858、860、862、864、866、868、870、872、874、876、878、880、882、884、886、888、890、892、894、896、898、900、902、904、906、908、910、912、914、916、918、920、922、924、926、928、930、932、933、934、935、936、937、938、939、940、941、942、943、944、945、947、949、951和952。在本发明文中,此表达盒通常包括可操作地连接到启动子序列的所选择的GAT多核苷酸和其它转录和翻译起始调控序列,其中调控序列足以引导GAT序列在被转化植物的特定组织(如整个植株、叶、根等)中的转录。
许多启动子可以被用于实施本发明。可以根据期望的结果来选择启动子。也就是说,核酸可以与组成型的、组织特异性的或者其他的启动子结合,用于在植物中进行表达。
组成型启动子包括,例如Rsyn7和在WO99/43838和美国专利6,072,050中描述的其他组成型启动子的核心启动子;核心CaMV 35S启动子(Odell等人(1985)Nature 313:810-812);水稻actin(McElroy等人(1990)Plant Cell 2:163-171);泛素(Christensen等人(1989)Plant Mol.Biol.12:619-632以及Christensen等人(1992)Plant Mol.Biol.18:675-689);pEMU(Last等人(1991)Theor.Appl.Genet.81:581-588);MAS(Velten等人(1984)EMBOJ.3:2723-2730);ALS启动子(美国专利5,659,026)等。其他的组成型启动子包括,例如在美国专利5,608,149;5,608,144;5,604,121;5,569,597;5,466,785;5,399,680;5,268,463;5,608,142和6,177,611中公开的那些启动子。
可用化学调控型启动子来通过使用外源性的化学调节剂调节基因在植物中的表达。根据目的不同,启动子可以是化学诱导型启动子,使用化学药品来诱导基因表达,或者可以是化学抑制性启动子,使用化学药品来抑制基因表达。化学诱导型启动子在本领域是已知的,包括,但是不限于,玉米In2-2启动子,其被苯磺胺除草剂安全剂激活;玉米GST启动子,其被用作发芽前的除草剂的疏水性亲电子化合物所激活;烟草PR-1a启动子,其被水杨酸激活。感兴趣的其他化学调控启动子包括类固醇响应性启动子。参见,例如,Schena等人(1991)Proc.Natl.Acad.Sci.USA 88:10421-10425的McNellis等人(1998)Plant J.14(2):247-257中描述的糖皮质激素诱导型启动子,以及四环素诱导型和四环素抑制型启动子,例如Gatz等人(1991)Mol.Gen.Genet.227:229-237和美国专利5,814,618和5,789,156,在此引入作为参考。
还可以用组织优选的启动子来将GAT表达靶向于特定的植物组织中。组织优选的启动子包括在Yamamoto等人(1997)Plant J 12(2):255-265;Kawamata等人(1997)Plan tCell Physiol.3(7):792-803;Hansen等人(1997)Mol.Gen Genet,254(3):337-343;Russell等人(1997)Transgenic Res.6(2):157-168;Rinehart等人(1996)Plant Physiol.112(3):1331-1341;VanCamp等人(1996)Plant Physiol.112(2):525-535;Canevascini等人(1996)Plant Physiol,112(2):513-524;Yamamoto等人(1994)Plant Cell Physiol.35(5):773-778;Lam(1994)ResultsProbl.Cell Differ.20:181-196;Orozco等人,(1993)Plant MolBiol,23(6):1129-1138;Matsuoka等人(1993)Proc Natl.Acad.Sci.USA 90(20):9586-9590;以及Guevara-Garcia等人(1993)Plant J 4(3):495-505中公开的那些启动子。如果需要,可以修饰这种启动子,来进行弱表达。
叶子特异性的启动子在本领域中是已知的。参见,例如Yamamoto等人(1997)Plant J.12(2):255-265;Kwon等人(1994)PlantPhysiol.105:357-67;Yamamoto等人(1994)Plant Cell Physiol.35(5):773-778;Gotor等人(1993)Plant J 3:509-18;Orozco等人(1993)Plant Mol,Biol,23(6):1129-1138;和Matsuoka等人(1993)Proc.Nat1.Acad.Sci.USA 90(20):9586-9590。
根的优选启动子是已知的,可从许多可获得的文献中选择或者重新从各种相容的物种中分离得到。参见,例如Hire等人(1992)PlantMol.Biol.20(2):207-218(大豆根特异性谷氨酸盐合成酶基因);Keller等人(1991)Plant Cell 3(10):1051-1061(法国菜豆GRP 1.8基因中的根特异性调控元件);Sanger等人(1990)Plant Mol,Biol.14(3):433-443(根癌农杆菌的甘露氨酸合酶(MAS)基因的根特异性启动子);以及Miao等人(1991)Plant Cell 3(1):11-22(编码胞质谷氨酸盐合成酶(GS)的全长cDNA克隆,其在大豆的根和根节结中表达)。还可以参见Bogusz等人(1990)Plant Cell2(7):633-641,其中公开两种从固氮的非豆科类Parasponiaandersonii和相关的非固氮的非豆科类Trema tomentosa的血色素基因中分离得到的根特异性启动子。将这些基因的启动子连接到β-葡萄糖苷酸酶报导基因上,导入非豆科类烟草(Nicotiana tabacum)和豆科Lotus corniculatus中,在这两种情形下,保留了根特异性启动子活性。Leach等人(1991)描述了他们对高度表达毛根农杆菌的rolC和rolD根诱导型基因的启动子的分析(参见Plant Science(Limerick)79(1):69-76)。他们的结论是,在这些启动子中,增强子和组织优选的DNA决定簇是相互分离的。Teeri等人(1989)EMBO J.8(2):343-350使用融合到lacZ上的基因融合物,证明编码章鱼碱合酶的农杆菌T-DNA基因在根尖表皮尤其活跃,在完整的植株中,TR2′基因是根特异性的,由叶肉组织中的创伤刺激,在与杀虫基因或杀幼虫基因一起使用时,这是特别期望的特征的组合。
融合到npt II(新霉素磷酸转移酶II)上的TR1′基因具有相似的特征。其他的根优选的启动子包括VfENOD-GRP3基因启动子(Kuster等人(1995)Plant Mol.Biol.29(4):759-772);ZRP2启动子(美国专利5,633,636);IFS1启动子(美国专利申请10/104,706)和rolB启动子(Capana等人(1994)Plant Mol.Biol.25(4):681-691)。还参见美国专利5,837,876;5,750,386;5,459,252;5,401,836;5,110,732;和5,023,179。
“种子优选的”启动子包括“种子特异性”启动子(在种子发育过程中具有活性的那些启动子,例如种子存储蛋白的启动子),以及“种子萌发的”启动子(在种子萌发过程中具有活性的启动子)。参见Thompson等人(1989)Bio Essays 10:108,在此引入作为参考。这种种子优选的启动子包括,但是不限于,Ciml(细胞分裂素诱导的信息);cZ19B1(玉米19kDa玉米蛋白);milps(myo-肌糖-1-磷酸合酶);以及celA(纤维素合酶)(参见美国专利6,225,529,在此引入作为参考)。玉米蛋白γ是一种胚乳特异性启动子。Glob-1是胚胎特异性启动子。对于双子叶植物来说,种子特异性启动子包括,但是不限于,大豆β-菜豆蛋白、油菜籽蛋白、β-伴大豆球蛋白、大豆凝集素、cruciferin等。对于单子叶植物来说,种子特异性启动子包括,但是不限于,玉米15kDa玉米蛋白、22kDa玉米蛋白、27kDa玉米蛋白、g-玉米蛋白、waxy、shrunken 1、shrunken 2、球蛋白1等。还可以参见WO 00/12733,其公开了来自end1和end2基因的种子优选启动子;在此引入作为参考。
特别地,可以使用引导GAT核酸在植物的所有组织中表达的强或者弱的组成型植物启动子。在大多数环境条件下和发育或者细胞分化的状态下,这种启动子是有活性的。除了上面提及的启动子外,组成型启动子的例子包括根瘤农杆菌的1’-或2’-启动子,来自各种植物基因的其他转录起始区是技术人员已知的。当本发明的GAT多肽的过度表达对植物有害时,技术人员将认识到可以使用弱的组成型启动子来进行低水平表达。总之,所述“弱启动子”是指以低水平驱使编码序列的表达的启动子。“低水平”是指水平为每个细胞从约1/1000转录本到约1/100,000转录本,到约低至1/500,000转录本。此外,应当认识到,弱启动子还包括仅在少数细胞中被表达而不在其他细胞中表达的启动子,产生总体的低水平表达。当启动子以不可接受的高水平表达时,可以删除或修饰启动子序列部分,来降低表达水平。当高水平表达对植物无害时,可以使用强启动子,如t-RNA或其它pol III启动子,或强pol II启动子(如花椰菜花叶病毒启动子、CaMV、35S启动子)。
或者,植物启动子可以处于环境控制之下。这种启动子被称为“诱导型”启动子。可能通过诱导型启动子改变转录的环境条件的例子包括病原体攻击、厌氧条件或光照的存在。在某些情况下,需要使用“组织特异的”和/或处于发育控制之下的启动子,这样GAT多核苷酸只在某些组织或其发育阶段,如叶、根和芽等中表达。与除草剂耐受性相关的基因的内源启动子和相关的表型对于驱动GAT核酸的表达特别有用,例如P450单加氧酶、谷胱甘肽-S-转移酶、同型谷胱甘肽-S-转移酶、草甘膦氧化还原酶和5-烯醇丙酮酰莽草酸盐-2-磷酸合酶。
也可用组织特异性启动子来引导异源结构基因,包括本文所述GAT多核苷酸的表达。因此,这种启动子可用于重组表达盒中,驱动期望在本发明的转基因植物中表达的任何基因的表达,例如GAT和/或其它赋予除草剂抗性或耐受性的基因,影响其它有用特征(如杂种优势)的基因。类似地,如来自5’调控序列或异源基因内含子的增强子元件,也可用来改善异源性结构基因,如GAT多核苷酸的表达。
总之,用于植物表达盒中的特定启动子取决于特定应用。能在植物细胞中引导转录的大量启动子的任何一种都是合适的。启动子可以是组成型或诱导型的。除了上述启动子,可在植物中起作用的细菌来源的启动子包括章鱼碱合酶启动子、胭脂碱合酶启动子和其它来源于Ti质粒的启动子。参见Herrera-Estrella等人(1993)Nature 303:209。病毒启动子包括CaMV的35S和19S RNA启动子。参见Odell等人(1985)Nature 313:810-812。其它的植物启动子包括核酮糖-l,3-二磷酸羧化酶小亚基启动子和菜豆蛋白启动子。也可以使用来自E8基因(参见Deikman和Fischer(1988)EMBO J.7:3315)和其它基因的启动子序列。还考虑了对单子叶植物种类特异的启动子(McElroy和Brettell(1994)“Foreign gene expression in transgeniccereals”Trends Biotech.12:62-68)。或者,采用本领域已知的方法,包括序列分析、增强子或启动子捕获等,可以从任何的病毒、细菌或植物来源中鉴定出具有有用特征的新启动子。
在制备本发明的表达载体时,也可以使用除启动子和GAT编码基因以外的序列。如果期望合适的多肽表达,聚腺苷酸化区域可来源于天然基因、各种其它植物基因或T-DNA。也可以使用信号/定位肽,这些肽例如能促进被表达的多肽转运进入内部细胞器(如叶绿体)或进行胞外分泌。
含有GAT多核苷酸的载体还可以包括赋予植物细胞以可选择表型的标记基因。例如,这种标记可以编码抗微生物剂耐受性、特定的抗生素耐受性,如对卡那霉素、G418、博来霉素、潮霉素的耐受性,或除草剂耐受性,例如对氨磺隆(chlorosulfuron)或者膦丝菌素的耐受性。可以使用报告基因,通过可视化反应产物(如β-葡糖醛酸糖苷酶、β-半乳糖苷酶和氨霉素乙酰转移酶)或通过直接可视化基因产物自身(如绿荧光蛋白、GFP;Sheen等(1995)The Plant Journal 8:777),来监测基因表达和蛋白质定位,用于例如监测植物细胞中的瞬时基因表达。例如,在筛选具有除草剂耐受性活性的植物细胞培养物时,可以将瞬时表达系统应用于植物细胞。
植物转化
原生质体
在本领域中可利用许多用于建立各种植物种类的可转化的原生质体以及随后转化培养的原生质体的方法,在此引入作为参考。例如参见Hashimoto等人(1990)Plant Physiol.93:857;Fowke和Constabel(编辑)(1994)Plant Protoplasts;Saunders等人(1993)Applicaiont of Plant In Vitro Technology symposium,UPM16-18;以及Lyznik等人(1991)Bio Techniques 10:295,在此引入作为参考。
叶绿体
叶绿体是一些除草剂耐受性活性的作用部位,在一些情况下,可将GAT多核苷酸融合到叶绿体转运序列肽上,以便将基因产物转运到叶绿体中。在这些情况下,可有利地将GAT多核苷酸转化到植物宿主细胞的叶绿体中。在本领域中有许多方法可实现叶绿体的转化和表达(例如可参见Daniell等人(1998),Nature Biotech 16:346;O’Neill等人(1993),The Plant Journal 3:729;Maliga(1993),TIBTECH11:1)。此表达构建体含有在植物中起作用的可操作地连接到编码GAT多肽的多核苷酸上的转录调节序列。设计用于在叶绿体中起作用的表达盒(如含有GAT多核苷酸的表达盒)使其包含确保在叶绿体中进行表达所需的序列。通常,此编码序列的两侧是叶绿体基因组的两个同源区域,以进行与叶绿体基因组的同源重组;可选择的标记基因还常常出现在侧面连接的质粒DNA序列中,以便在所生成的转原生质体(transplastonic)植物细胞中选择遗传稳定的转化叶绿体(例如,可参见Maliga(1993)和Daniell(1998),及其引用的参考文献)。
通用的转化方法
可采用各种常规的技术,将本发明的DNA构建体导入期望的植物宿主的基因组中。用于转化各种高等植物物种的技术是熟知的,并在科技文献中有描述。参见,例如Payne、Gamborg、Croy、Jones等,皆同上文,以及如Weising等人(1988)Ann.Rev.Genet.22:421以及美国专利5,889,191、5,889,190、5,866,785、5,589,367、5,316,931,在此引入作为参考。
各种其他的转化方法也包括在本发明中。转化操作以及用于将核苷酸序列导入到植物中的操作可能根据用于转化的植物或植物细胞的类型(即单子叶植物或双子叶植物)而改变。适合将核苷酸序列导入植物细胞中并接着插入到植物基因组中的方法包括显微注射(Crossway等人(1986)Biotechniques 4:320-334)、电穿孔(Riggs等人(1986)Proc.Natl.Acad.Sci.USA 83:5602-5606)、农杆菌介导的转化(美国专利5,563,055和5,981,840)、直接的基因转移(Paszkowski等人(1984)EMBO J.3:2717-2722)和弹道粒子加速(参见,例如美国专利4,945,050;美国专利5,879,918;5,886,244;5,932,782;Tomes等人(1995)“通过显微注射轰击直接将DNA转移到完整的植物细胞中”,Plant Cell,Tissue,and OrganCulture:Fundamental Methods,Gamborg和Phillips编辑(Springer-Verlag,柏林);McCabe等人(1988)Biotechnology6:923-926);以及Lecl转化(WO00/28058)。还可以参见Weissinger等人(1988)Ann.Rev.Genet 22:421-477;Sanford等人(1987)Particulate Science and Technology 5:27-37(洋葱);Christou等人(1988)Plant Physiol.87:671-674(大豆);McCabe等人(1988)BiolTechnology 6:923-926(大豆);Finer和McMullen(1991)In vitro Cell Dev.Biol.27P:175-182(大豆);Singh等人(1998)Theor.Appl.Genet.96:319-324(大豆);Datta等人(1990)Biotechnology 8:736-740(水稻);Klein等人(1998)Proc.Natl.Acad.Sci.USA 85:4305-4309(玉米);Klein等人(1988)Biotechnology 6:559-563(玉米);美国专利5,240,855;5,322,783;5,324,646;Klein等人(1988)Plant Physiol.91:440-444(玉米);Fromm等人(1990)Biotechnology 8:833-839(玉米);Hooykass-Van Slogteren等人(1984)Nature(伦敦)311:763-764;美国专利5,736,369(谷);Bytebier等人(1987)Proc.Natl.Acad.Sci.USA 84:5345-5349(百合);De Wet等人(1985)The Experimental Manipulation of Ovule Tissue,Chapman等人编辑(Longman,纽约),197-209(花粉);Kaeppler等人(1990)Plant Cell Reports 9:415-418和Kaeppler等人(1992)Theor.Appl.Genet.84:560-566(根须介导的转化);D’Halluiin等人(1992)Plant Cell 4:1495-1505(电穿孔);Li等人(1993)Plant Cell Reports 12:250-255和Christon和Ford(1995)Annalsof Botany 75:407-413(水稻);Osjoda等人(1996)NatureBiotechnology 14:745-750(通过根癌农杆菌转化的玉米),全部在此引入作为参考。
例如,可采用电穿孔和显微注射植物细胞原生质体而将DNA直接导入植物细胞的基因组DNA中,或者可使用弹道法,如DNA微粒轰击来将DNA构建体直接导入植物组织中。或者,可使该DNA构建体与合适的T-DNA侧翼区域组合,然后一起导入常规的根癌农杆菌宿主载体中。当用该细菌感染细胞时,农杆菌宿主的毒力功能将指导该构建体和相邻标记插入到植物细胞的DNA中。
显微注射技术在本领域中是已知的,并且在科学和专利文献中有很好的描述。Paszkowski等人(1984)MEBO J 3:2717中描述了使用聚乙二醇沉淀导入DNA构建体。Fromm等人(1985)Proc.Natl.Acad.Sci.USA 82:5824中描述了电穿孔技术。Klein等人(1987)Nature 327:70和Week等人(1993)Plant Physiol102:1077描述了弹道转化技术。
在一些实施方案中,采用农杆菌介导的转化技术,将本发明的GAT序列转移到转基因植物中。农杆菌介导的转化广泛地用于双子叶植物的转化,但是,某些单子叶植物也可用农杆菌转化。例如,以下文献描述了稻米的农杆菌转化:Hiei等人(1994)Plant J.6:271;美国专利5187073;美国专利5,591,616;Li等人(1991)Science inChina 34:54;和Raineri等人(1990)Bio/Technology 8:33。此外,用农杆菌感染转化玉米、大麦、黑小麦和芦笋也被描述(Xu等人(1990)Chinese J Bot 2:81)。
农杆菌介导的转化技术利用根癌农杆菌的肿瘤诱导(Ti)性质粒整合到植物细胞基因组中的能力,将感兴趣的核酸共转染到植物细胞中。通常,制备一种表达载体,其中感兴趣的核酸,如本发明的GAT多核苷酸被连接到自主复制的质粒上,该质粒还含有T-DNA序列。T-DNA序列通常位于感兴趣的表达盒核酸的侧面,并含有该质粒的整合序列。除了该表达盒外,T-DNA通常还含有标记序列,如抗生素抗性基因。然后将具有T-DNA和此表达盒的质粒转染到根癌农杆菌细胞中。通常,为了有效地转化植物细胞,根癌农杆菌在质粒上含有必需的vir区域,或者使该区域整合进染色体中。关于根癌农杆菌的讨论,可参见Firoozabady和Kuehnle,(1995)Plant Cell Tissue and OrganCulture Fundamental Methods,Gamborg和Phillips(编辑)。
在一些实施方案中,本发明的多核苷酸增加了感兴趣的多核苷酸序列的任何组合,以生成具有期望表型的植物。例如,本发明的多核苷酸添加了任何其他的编码具有杀虫的和/或杀虫活性的多肽的多核苷酸,例如苏云金芽孢杆菌(Bacillus thuringiensis)毒性蛋白(在美国专利5,366,892;5,747,450;5,737,514;5,723,756;5,593,881;和Geiser等人(1986)Gene 48:109中描述)、凝集素(Van Damme等人(1994)Plant Mol.Biol.24:825、pentin(在美国专利5,981,722中描述)等。产生的组合还可以包括多拷贝的感兴趣的多核苷酸的任何之一。本发明的多核苷酸还可以添加任何其他基因或者基因的组合,产生具有多种期望特性组合的植物,这些特性包括,但是不限于,期望的用于动物饲养的特性,例如高脂肪基因(例如,美国专利6,232,529);平衡氨基酸(例如hordothionin(美国专利5,990,389;5,885,801;5,885,802和5,703,409);大麦高赖氨酸(Williamson等人(1987)Eur.J.Biochem.165:99-106和WO98/20122)和高甲硫氨酸的蛋白质(Pedersen等人(1986)J.Biol.Chem.261:6279;Kirihara等人(1988)Gene 71:359;和Musumura等人(1989)Plant Mol.Biol.12:123));提高的消化性(例如,修饰的存储蛋白(2001年11月7日申请的美国专利申请10/053,410);和硫氧还蛋白(2001年12月3日申请的美国专利申请10/005,429));该专利的公开在此引入作为参考。
本发明的多核苷酸还可以添加所期望的用于疾病或者除草剂抗性的特性(例如,串珠镰孢菌素解毒基因(美国专利5,792,931);毒性和疾病抗性基因(Jones等人(1994)Science 266:789;Martin等人(1993)Science 262:1432;Mindrinos等人(1994)Cell78:1089);会产生对除草剂抗性的乙酰乳酸合酶(ALS)突变体例如S4和/或Hra突变体;谷氨酰胺合酶抑制剂例如草酊磷(phosphinothricin)或者basta(例如bar基因);和草甘膦抗性(EPSPS基因));和用于处理产品期望的特性如高油(例如美国专利6,232,529);改良的油(例如脂肪酸饱和酶基因(美国专利5,952,544;WO 94/11516));修饰的淀粉(例如ADPG焦磷酸酶(AGPase)、淀粉合酶(SS)、淀粉分支酶(SBE)和淀粉去分支酶(SDBE));和聚合体或者原生质体(例如,美国专利5.602,321;β-酮硫解酶、多聚羟基丁酸盐合酶和乙酰CoA还原酶(Schubert等人(1988)J Bacteriol.170:5837-5847),便于多羟基链烷酸盐(PHAs)的表达);它们的公开在此引入作为参考。还可以将本发明的多核苷酸与提供农业特性的多核苷酸组合,如雄性不育(例如,参见美国专利5,583,210)、茎杆强度、开花时间,或者转化技术特性例如细胞周期调控或者基因打靶(例如,WO 99/61619,WO 00/17364和WO99/25821);其公开在此引入作为参考。
可以通过任何方法来产生这些累积的组合,包括但是不限于,通过任何常规或者TopCross方法,或者遗传转化来杂交植物。如果通过遗传转化植物来累积特性,可以在任何时间和以任何顺序来组合感兴趣的多核苷酸序列。例如,包含一种或多种期望特性的转基因植物被用作目标,通过随后的转化引入另一种特性。可以在共转化操作中,与由任何转化表达盒的组合提供的任何感兴趣的多核苷酸一起引入该特性。例如,如果导入两条序列,可以将两条序列包含在分开的转化表达盒(反式)或者包含有在同一转化表达盒(顺式)中。通过相同的启动子或者通过不同的启动子来驱动序列的表达。在某些情形下,期望导入一种转化表达盒,其抑制感兴趣的核苷酸的表达。这可以与任何的其他抑制表达盒或者过度表达盒的组合一起组合,在植物中产生期望的特性组合。还应当认识到,可以采用点特异性重组系统,在期望的基因组位置上累积该多核苷酸序列。参见,例如WO99/25821、WO99/25854、WO99/25840、WO99/25855和WO99/25853,它们均在此引入作为参考。
转基因植物的再生
可以培养通过植物转化技术(包括上面讨论的那些)得到的转化植物细胞,以至产生具有转化的基因型(即GAT多核苷酸)从而具有期望表型(如所需的对草甘膦或草甘膦类似物的抗性(即耐受性))的完整植物。这种再生技术依赖于操纵组织培养生长培养基中的某种植物激素,通常依赖于已与所需的核苷酸序列一起导入的杀生物剂和/或除草剂标记物。对于玉米的转化和再生,参见Gordon-Kamm等人,ThePlant Cell,2:603-618(1990)。或者,可以对本发明GAT多核苷酸赋予的对草甘膦抗性进行选择。由培养原生质体再生植物被描述在Evans等人(1983)Protoplasts Isolation and Culture,Handbook of PlantCell Culture,第124-176页,Macmillian出版公司,纽约;和Binding(1985)Regeneration of Plants,Plant Protoplasts,第21-73页,CRC出版社,Boca Raton。还可从植物愈伤组织、外植体、器官或其部分中获得再生。这些再生技术被一般性地描述在Klee等人(1987)Ann.Rev.of Plant Phys 38:467中。还可参见Payne和Gamborg。
将转化的植物细胞、愈伤组织(calli)或者外植体培养在再生培养基中,在黑暗中数周,通常约1-3周,使体细胞胚成熟。优选的再生培养基包括含有MS盐的培养基。然后,在光照/黑暗循环中,将植物细胞、愈伤组织(calli)或者外植体培养在生根培养基中,直到发育成幼芽和根。用于植物再生的方法在本领域是已知的,优选方法提供在Kamo等人(Bot.Gaz.146(3):324-334,1985);West等人(The Plant Cell 5:1361-1369,1993)和Duncan等人(Planta 165:322-332,1985)。
然后,将小的植物幼芽转移到含有生根培养基的试管中,再生长约1周和发育出更多的根。然后,将该植物转移到温室的盆中的土壤混合物中。
可以如Horsch等人Science,227:1229-1231(1985)和Fraley等人Proc.Natl.Acad.Sci.U.S.A.,80:4803(1983)所描述,来实现再生含有通过农杆菌导入的外源基因的植物。这种方法通常在2-4周内生成幼芽,然后,将这些转化体幼芽转移到合适的诱导根的培养基中,该培养基含有可选择试剂和抗生素,以防止细菌生长。本发明的转基因植物可以是能够繁育的或者不育的。
还可以由愈伤组织、外植体、器官或其部分实现再生。这种再生技术通常被描述在Klee等人,Arasz.Rev.of Plant Phys.38:467-486(1987)中。从简单的植物原生质体或者各种外植体再生植物是本领域已知的。参见,例如Methods for Plant Molecular Biology,A.Weissbach和H.Weissbach编辑,学院出版社公司,圣地亚哥,加利福尼亚州(1988)。对于玉米细胞的培养和再生,通常参见,The MaizeHandbook,Freeling和Walbot编辑,Springer,纽约(1994);Cornand Corn Improvement,第3版,Sprague和Dudley编辑,AmericanSociety of Agronomy,麦迪逊,威斯康星州(1988)。
在用农杆菌转化后,将外植体转移到选择培养基中。技术人员将认识到,选择培养基的选择依赖于被共转染到该外植体中的可选择标记。经过适当长的时间后,转化于将开始形成幼芽。在幼芽长约1-2厘米后,将其转移到合适的根和幼芽培养基中。应该在根和幼芽培养基中维持选择压力。
通常,在1周到约2周中,转化子长出根并形成小植株。在小植株高约3-5厘米后,可将它们外植在纤维罐(fiber pot)中的无菌土壤中。本领域技术人员将认识到,应使用不同的气候环境以获得不同的的转化植物。例如,在生成根和幼芽后,将转化植物的切片以及体细胞胚转移到用于建立小植株的培养基中。关于转化植物的选择和再生的描述,可参见Dodds和Roberts(1995)Experiments in PlantTissue Culture,第3版,剑桥大学出版社。
还有采用真空渗滤(Bechtold N.,Ellis J.和Pelletier G.,1993,In planta Agrobacterium mediated gene transfer byinfiltration of adult Arabidopsis thaliana plants.CR Acad SciParis Life Sci 316:1194-1199)和简单浸渍开花植物(Desfeux,C.,Clough S.J.和Bent A.F.,2000,Female reproductive tissueare the primary target of Agrobacterium-mediatedtransformation by the Arabidopsis floral-dip method.PlantPhysiol.123:895-904)进行农杆菌转化拟南芥属的方法。采用这些方法,无需组织培养就可以产生转基因种子。
对于一些植物品种,还未研究出有效的农杆菌介导的转化方法。例如,对于一些最有经济价值的棉花品种,还未见报道与再生的转化组织偶联来产生转基因植物成功的组织转化。然而,可用于这些植物的方法包括通过农杆菌介导的转化稳定地将多核苷酸引入有关植物品种中,确认可操作性,然后用标准有性杂交或回交技术,将转基因转移到期望的经济作物品种中。例如,如果是棉花,可以用农杆菌来转化陆地棉(Gossypium hirustum)的Coker品系(如Coker品系310,312,5110 Deltapine 61或Stoneville 213),然后通过回交,将转基因引入其它更具经济价值的陆地棉品种中。
可以从基因型或表型上来表征本发明的转基因植物,来确定存在本发明GAT多核苷酸。可以用任何一种熟知的技术进行基因型分析,包括对基因组DNA的PCR扩增以及将基因组DNA与特异性标记的探针杂交。表型分析包括,例如,暴露在一种经过选择的除草剂(如草甘膦)中的植物或植物组织的存活力。
本领域技术人员将会认识到,在将含有GAT基因的表达盒稳定地整合到转基因植物中并且被证实是可操作的之后,可以通过有性杂交导入到其他植物中。可以采用大量的标准繁育技术的任何一种,这取决于将要杂交的种类。
在生长传代的农作物中,成熟的转基因植物通过收割或者组织培养技术来繁育,生成多个相同的植物。选择期望的转基因植物,获得新品种,通过生长繁育用于商业目的。在种子繁育的农作物中,成熟的转基因植物进行自交,生成纯合的纯种植物。这些纯种植物生成含有新导入的异源核酸的种子。种植这些种子,生成将会产生所选择的表型的植物。
从再生植物上获得的部分,例如花、种子、叶子、枝条、果实等包括在本发明中,只要这些部分包含含有分离的GAT核酸的细胞。再生植物的后代和变体以及突变体也包括在本发明的保护范围内,条件是这些部分包括导入的核酸序列。
通过标准的免疫印迹和DNA检测技术,针对对GAT核酸的传播筛选表达可选择标记的转基因植物。通常还在异源核酸的表达水平上,对转基因品系进行评价。首先在RNA水平上确定表达以鉴定和量化阳性表达植物。采用用于RNA分析的标准技术,包括使用被设计来仅扩增异源RNA模板的寡核苷酸引物的PCR扩增分析和使用异源核酸特异探针的溶液杂交分析。然后,使用本发明的特异性反应抗体,通过Western印迹分析来分析RNA阳性植物的蛋白质表达。此外,分别使用异源核酸特异的多核苷酸探针和抗体,进行按照标准操作的原位杂交和免疫细胞化学,从而在转基因组织内定位表达部位。通常,对大量转基因品系中的插入核酸进行筛选,来鉴定和选择具有最合适的表达模式的植物。
优选的实施方案是与所添加的异源核酸纯合的转基因植物;即含有两条所添加的核酸序列的转基因植物,在染色体对的各个染色体的相同基因座上各有一个基因。可以通过有性杂交(自交)和那样单一的添加的异源核酸的杂合转基因植物来获得纯合的转基因植物,使所生成的种子发芽,分析所生成的植物中相对于对照植物(即天然的非转基因的)的改变的细胞分化。还包括与亲本植物回交,以及与非转基因植物进行远交。
基本上任何植物都可使用本发明的GAT多核苷酸来进行转化。用于转化和表达本发明的新颖的GAT多核苷酸的合适植物包括农艺上和园艺上重要的种类。这些种类包括但不限于这些家族的成员:禾本科(包括玉米、黑麦、黑小麦、大麦、粟、稻、小麦、燕麦等);豆科(包括豌豆、菜豆、扁豆、花生、薯蓣豆、豇豆、绒毛黧豆、大豆、苜蓿、紫苜蓿、羽扇豆、野豌豆、荷花、草木樨、紫藤和香豌豆);菊科(维管植物的最大家族,包括至少1000种属,包括重要的经济作物,如向日葵)和蔷薇科(包括悬钩子、杏树、杏仁、桃树、玫瑰等)。以及坚果树(包括胡桃、山核桃、榛子等),和林木〔包括松属(Pinus)、栎属(Quercus)、黄杉属(Pseudotsuga)、红杉属(Sequoia)、杨属(Populus)等〕。
其它可用本发明的GAT多核苷酸修饰的目标植物以及上述那些包括下述种属的植物:剪股颖属(Agrostis)、葱属(Allium)、金鱼草属(Antirrhinum)、旱芹属(Apium)、拟南芥属(Arabidopsis)、花生属(Arachis)、芦笋属(Asparagus)、颠茄属(Atropa)、燕麦属(Avena)(如燕麦)、山白竹属(Bambusa)、芸苔属(Brassica)、雀麦属(Bromus)、博洛瓦阿拉属(Browaalia)、山茶属(Camellia)、大麻属(Cannabis)、辣椒属(Capsicum)、鹰嘴豆属(Cicer)、藜属(Chenopodium)、菊苣属(Chiehorium)、柑桔属(Citrus)、咖啡属(Coffea)、薏苡属(Coix)、香瓜属(Cucumis)、葫芦属(Curcubita)、狗牙根属(Cynodon)、鸭茅属(Dactylis)、曼陀罗属(Datura)、胡萝卜属(Daucus)、毛地黄属(Digitalis)、薯蓣属(Dioscorea)、油棕属(Elaeis)、Eleusine、羊矛属(Festuca)、草莓属(Fragaria)、老鹳草属(Geranium)、大豆属(Glycine)、向日葵属(Helianthus)、龙骨角属(Heterocallis)、三叶胶属(Hevea)、大麦属(Hordeum)(如大麦)、天仙子属(Hyoscyamus)、番薯薯(Ipomoea)、莴苣属(Lactuca)、兵豆属(Lens)、百合属(Lilium)、亚麻属(Linum)、黑麦草属(Lolium)、百脉根属(Lotus)、番茄属(Lycopersicon)、甘牛至属(Majorana)、苹果属(Malus)、芒果属(Mangifera)、木薯属(Manihot)、苜蓿属(Medicago)、龙面花属(Nemesia)、烟草属(Nicotiana)、驴食豆属(Onobrychis)、稻属(Oryza)(如稻)、稷属(Panicum)、天竺葵属(Pelargonium)、狼尾草属(Pennisetum)(如粟)、碧冬茄属(Petunia)、豌豆属(Pisum)、菜豆属(Phaseolus)、梯牧草属(Phleum)、早熟禾属(Poa)、李属(Prunus)、毛茛属(Ranunculus)、萝卜属(Raphanus)、茶庶子属(Ribes)、蓖麻属(Ricinus)、悬钩子属(Rubus)、甘蔗属(Saccharum)、蛾蝶花属(Salpiglossis)、黑麦属(Secale)(如黑麦)、千里光属(Senecio)、狗尾草属(Setaria)、白芥属(Sinapis)、茄属(Solanum)、蜀黍属(Sorghum)、钝叶草属(Stenotaphrum)、可可树属(Theobroma)、车轴草属(Trifolium)、胡卢巴属(Trigonella)、小麦属(Triticum)(如小麦)、野豌豆属(Vicia)、豇豆属(Vigna)、葡萄属(Vitis)、玉蜀黍属(Zea)(如玉米)、Olyreae、Pharoideae以及其它。应注意的是,禾本科(Graminae)的植物是本发明方法的特选目标植物。
作为本发明的目标的普通农作物包括玉米、稻米、黑小麦、黑麦、棉花、大豆、高粱、小麦、燕麦、大麦、粟、向日葵、加拿大油菜、豌豆、菜豆、小扁豆、花生、薯蓣豆、豇豆、绒毛黧豆、大豆、苜蓿、紫苜蓿、羽扇豆、野豌豆、荷花、草木樨、紫藤和香豌豆、番茄、香蕉和坚果植物(如胡桃、山核桃等)。
一方面,本发明提供了一种方法,通过在可使农作物植株生产出农作物的条件下,种植由于转化了编码草甘膦N-乙酰转移酶基因而耐受草甘膦的农作物植株,来制造农作物和收获农作物。优选地,给植株或在植株附近施用草甘膦,其浓度足以控制杂草而不抑制转基因农作物植株生长和生产农作物。可以在种植之前,或在种植后的任何时间包括收获时施用草甘膦。草甘膦可以施用一次或多次。草甘膦施用的时间安排、用量、施用模式和其它参数将根据农作物植株和生长环境的具体性质而改变,本领域技术人员不难确定。本发明还提供了用这种方法制造的农作物。
本发明提供了繁殖含有GAT多核苷酸转基因的植物的方法。这种植物可以是,例如,单子叶植物或双子叶植物。一方面,繁殖包括将含有GAT多核苷酸转基因的植物和第二种植物杂交,这样至少一些杂交后代显示草甘膦抗性。
一方面,本发明提供了选择性地控制种植农作物的田间的杂草的方法。此方法包括种植由于转化了编码GAT(如GAT多核苷酸)的基因而具有草甘膦耐受性的农作物种子或植株,然后对农作物和任何杂草施用足量的草甘膦,以控制杂草而对农作物没有明显不利影响。重要的是应注意,农作物不必完全对除草剂不敏感,只要从抑制杂草得到的好处超过草甘膦或草甘膦类似物对农作物或农作物植株的不利影响即可。
另一方面,本发明提供了将GAT多核苷酸用作可选择标记基因的用途。在本发明的一个实施方案中,细胞或生物体中GAT多核苷酸的存在赋予了该细胞或生物体以草甘膦耐受性的可检测特征,从而使技术人员可以挑选已用与GAT多核苷酸连接的感兴趣基因转化的细胞或生物体。因此,例如,可将GAT多核苷酸导入核酸构建体中,如载体中,由此可通过在草甘膦存在时培养该宿主,选择成活和/或生长能力明显高于缺乏此核酸构建体的宿主存活或生长速度的植株,以鉴定含有此核酸构建体的宿主(如细胞或转基因植物)。在许多对草甘膦敏感的宿主中,包括植物、大多数细菌(包括大肠杆菌)、放线菌、酵母、藻类和真菌,GAT多核苷酸都可用作一种可选择标记。就抗常规的抗生素抗性而言,采用除草剂抗性作为植物中一种标记的一个好处是,它消除了某些人对抗生素抗性可能泄漏到环境中的担心。在本说明书的实施例部分描述了一些实验数据,这些实验显示了GAT多核苷酸在各种宿主系统中作为可选标记的应用。
GAT多核苷酸的选择赋予转基因植物以增强的草甘膦抗性
在按照本文所述方法多样化的GAT编码核酸的文库中,进行可赋予转基因植物以草甘膦抗性的能力的选择。经过一轮或多轮多样化和选择后,可采用修饰的GAT基因作为选择标记,以便生产和评价转基因植物,还可用作赋予实验作物或农作物以除草剂抗性的方法。例如,在将SEQ ID NO:1-SEQ ID NO:5中的一个或多个多样化以产生一个多样化的GAT多核苷酸文库后,可以通过在大肠杆菌中表达该GAT编码序列文库,来进行最初的功能评价。如上所述,可纯化或部分纯化表达的GAT多肽,并用质谱仪筛选具有改进的动力学的菌株。经一轮或几轮初步多样化和选择后,将编码改进的GAT多肽的多核苷酸克隆入植物表达载体,将植物表达载体连接到,例如,强组成型启动子,如CaMV 35S启动子上。通常经衣杆菌介导的转化,将含有修饰的GAT核酸的表达载体转化入拟南芥宿主植株。例如,将花序浸在农杆菌溶液中,然后使它们生长并结籽,可容易地转化拟南芥属宿主。在大约6周内回收数千粒种子。然后从浸过的植物中大批收集种子并使其生长在土壤中。用这种方法,可以产生数千株独立转化的植株,以供评价、建立高产量(HTP)植物转化模式。用草甘膦喷洒成批生长的幼芽,具有草甘膦抗性的存活幼芽可在选择过程中存活,而非转基因的植株和掺入了不太有利修饰的GAT核酸的植株由于除草剂处理而被伤害或被杀死。任选地,例如可以利用该文库插入物侧面的T-DNA引物,通过PCR扩增来回收可赋予改善的草甘膦抗性的GAT编码核酸,并将其用于进一步的多样化步骤或用来产生相同或不同种类的其他转基因植物。如果需要,可以在各个随后的选择中,采用浓度增加的草甘膦,再进行多轮多样化和选择。用这种方法,可以获得在农田情况下有用的赋予草甘膦浓度抗性的GAT多核苷酸和多肽。
除草剂抗性
本发明提供一种组合物,其包括两种或多种本发明的多核苷酸。优选地,GAT多核苷酸编码具有不同动力学参数的GAT多肽,即可以将具有较低Km的GAT变体与具有较高k催化的变体组合。在另一个实施方案中,不同的GAT多核苷酸与叶绿体转运序列或者其他信号序列连接,从而在不同的细胞腔室、细胞器中表达GAT多肽或分泌一种或多种GAT多肽。
可以将本发明的草甘膦抗性机制与本领域已知的其它草甘膦抗性模式结合,以制造具有较高草甘膦抗性的植物和植物的外植体。例如,通过在植物基因组中插入具有产生高水平5-烯醇丙酮酰莽草酸-3-磷酸酯合酶(EPSP)能力的序列,生成耐受草甘膦的植物,这在以下美国专利:6,248,876B1、5,627,061、5,804,425、5,633,435、5,145,783、4,971,908、5,312,910、5,188,642、4,940,835、5,866,775、6,225,114 B1、6,130,366、5,310,667、4,535,060、4,769,061、5,633,448、5,510,471、Re.36,449、RE 37,287E和5,491,288以及国际出版物WO 97/04103、WO 00/66746、WO 01/66704和WO 00/66747中有更加完整的描述,出于所有目的,在此将它们全文引入作为参考。还可向表达编码草甘膦氧化-还原酶基因的植物传递草甘膦抗性,这在美国专利5,776,760和5,463,175中有更加完整的描述,出于所有目的,在此将它们全文引入以供参考。
此外,本发明的草甘膦抗性机制可以和除草剂抗性的其它模式结合,以使植物和植物外植体对草甘膦和一种或多种其它除草剂具有抗性。例如,羟苯基丙酮酰二氧化酶是催化对-羟苯基丙酮酸(HPP)转化成尿黑酸的反应的酶。抑制此酶并与此酶结合以抑制HPP转化为尿黑酸的分子可用作除草剂。对某些除草剂抗性更强的植物描述在美国专利6,245,968 B1、6,268,549和6,069,115以及国际出版物WO99/23886中,出于所有目的,在此将它们全文引入作为参考。
磺酰脲和咪唑啉酮除草剂通过阻遇乙酰乳糖合酶(ALS)或乙酰羟酸合酶(AHAS),也抑制高等植物的生长。磺酰脲和咪唑啉酮抗性植物的生产在美国专利5,605,011、5,013,659、5,141,870、5,767,361、5,731,180、5,304,732、4,761,373、5,331,107、5,928,937和5,378,824以及国际出版物WO 96/33270中有更加完整的描述,出于所有目的,在此将它们全文引入作为参考。
谷氨酸合成酶(GS)似乎是大多数植物细胞发育和生长必需的基础酶。GS的抑制剂对植物细胞是有毒的。根据GS抑制植物的毒性作用,已经开发了草铵膦除草剂。这些除草剂是非选择性的。它们抑制现有植物所有不同种类的生长,引起它们整体毁灭。对含有外源膦丝菌素乙酰转移酶的植物的开发描述在美国专利5,969,213、5,489,520、5,550,318、5,874,265、5,919,675、5,561,236、5,648,477、5,646,024、6,177,616B1和5,879,903中,出于所有目的,在此将它们全文引入作为参考。
原卟啉原氧化酶(protox)是产生植物存活必需的叶绿素所必需的。protox酶是许多除草化合物的靶标。这些除草剂也抑制现有植物所有不同种类的生长,引起它们整体毁灭。耐受这些除草剂的具有改变的protox活性的植物的开发描述在美国专利6,288,306 B1、6,282,837 B1和5,767,373中,出于所有目的,在此全文引入作为参考。
因此,本发明提供用于选择性地控制具有农作物的田间的杂草的方法,包括在田间种植由于转化了编码草甘膦N-乙酰转移酶的基因而耐受草甘膦的农作物种子或植物,给田间的农作物和杂草施用足量的草甘膦,以控制杂草而不显著地影响农作物。
本发明还提供一种方法,以控制田间的杂草并且防止在具有农作物的田间出现草甘膦抗性杂草,包括在田间种植农作物种子或植物,它们由于转化了编码草甘膦N-乙酰转移酶的基因和编码通过另一种途径赋予草甘膦耐受性的多肽的基因而耐受草甘膦,给田间的农作物和杂草施用足量的草甘膦,以控制杂草而不显著地影响农作物,所述另一种多肽如耐受草甘膦的5-烯醇丙酮酰莽草酸-3-磷酸酯合酶和/或耐受草甘膦的草甘膦氧化还原酶。
在另一个实施方案中,本发明提供用于控制田间杂草和防止在具有农作物的田间出现除草剂抗性杂草的方法,包括在田间种植农作物种子或植物,它们由于转化了编码草甘膦N-乙酰转移酶的基因和编码通过另一种途径赋予草甘膦耐受性的多肽(如耐受草甘膦的5-烯醇丙酮酰莽草酸-3-磷酸酯合酶和/或耐受草甘膦的草甘膦氧化还原酶)的基因和编码赋予对其他除草剂(例如突变的羟苯基丙酮酸二氧化酶、耐受磺胺的乙酰乳酸合酶、耐受磺胺的乙酰羟基酸合酶、耐受咪唑啉酮的乙酰乳酸的合酶、耐受咪唑啉酮的乙酰羟基酸合酶、膦丝菌素乙酰转移酶和突变的原卟啉原氧化酶)耐受性的基因而耐受草甘膦,给田间的农作物和杂草施用足量的草甘膦和其他除草剂(例如羟苯基丙酮酸二氧化酶抑制剂、磺胺、咪唑啉酮、双丙氨膦、膦丝菌素、唑啶草酮、氟丙嘧草酯、硫酸盐、草铵膦和原卟啉原氧化酶抑制剂),以控制杂草而不显著地影响农作物。
本发明还提供用于控制田间杂草和防止在具有农作物的田间出现除草剂抗性杂草的方法,包括在田间种植农作物种子或植物,它们由于转化了编码草甘膦N-乙酰转移酶的基因和编码赋予对其他除草剂(例如突变的羟苯基丙酮酸二氧化酶、耐受磺胺的乙酰乳酸合酶、耐受磺胺的乙酰羟基酸合酶、耐受咪唑啉酮的乙酰乳酸合酶、耐受咪唑啉酮的乙酰羟基酸合酶、膦丝菌素乙酰转移酶和突变的原卟啉原氧化酶)耐受性的基因而耐受草甘膦,给田间的农作物和杂草施用足量的草甘膦和其他除草剂(例如羟苯基丙酮酸二氧化酶抑制剂、磺胺、咪唑啉酮、双丙氨膦、膦丝菌素、唑啶草酮、氟丙嘧草酯、硫酸盐、草铵膦和原卟啉原氧化酶抑制剂),以控制杂草而不显著地影响农作物。
实施例
如下实施例是说明性的,而非限制性的。技术人员将认识到,可以改变许多非关键性的参数以获得实质上类似的结果。
实施例1:分离新的天然GAT多核苷酸
通过表达克隆来自具有GAT活性的杆菌菌株的序列,发现了五种天然GAT多核苷酸(即在非遗传修饰的生物中天然存在的GAT多核苷酸)。它们的核苷酸序列被确定,并在此以SEQ ID N0:1-5提供。简单地说,就对N-乙酰草甘膦的天然能力,筛选了大约500株杆菌和假单胞菌菌株。将菌株在LB中培养过夜,离心收集,在稀释的甲苯中渗透,然后洗涤并重悬在含有缓冲液、5mM草甘膦和200μM乙酰-CoA的反应混合物中。将细胞在反应混合物中培养1-48小时,培养结束后在反应物中加入等体积的甲醇。然后离心沉淀细胞,滤去上清液,然后用母离子型质谱仪进行分析。如图2所述,通过比较反应混合物与N-乙酰草甘膦标准品的质谱图,将反应产物阳性地鉴定为N-乙酰草甘膦。产物检测同时依赖于两种底物(乙酰CoA和草甘膦),并通过加热变性细菌的细胞而停止。
然后通过功能性筛选,从鉴定的菌株中克隆了各个GAT多核苷酸。制备基因组DNA并用Sau3A1酶部分消化。将大约4Kb的片段克隆入大肠杆菌表达载体中,并将其转化入电感受态大肠杆菌。在上述反应后,用质谱仪鉴定各个显示GAT活性的克隆,但以PMBS渗透替代甲苯。对基因组片段进行测序,并鉴定假定的GAT多肽编码的开放阅读框。在大肠杆菌中表达该开放阅读框,以及检测到在反应混合物中产生高水平的N-乙酰草甘膦,证实了对GAT基因的鉴定结果。
实施例2:分离自地衣芽孢杆菌菌株B6的GAT多肽的表征
纯化地衣芽孢杆菌菌株B6的基因组DNA,用Sau3A1部分消化,并将1-10Kb的片段克隆入大肠杆菌表达载体中。通过质谱分析,确定了赋予大肠杆菌草甘膦N-乙酰转移酶(GAT)活性的带有2.5kb插入物的克隆。此插入物的测序揭示了一个含有441个碱基对的完整开放阅读框。此开放阅读框随后的克隆证实它编码GAT酶。图4显示质粒pMAXY2120。将编码B6 GAT酶的基因转化入大肠杆菌XL1 Blue品系中。在Luria肉汤中加入10%无害的饱和培养物,将该培养物在37℃培养1小时。以1mM的浓度加入IPTG,诱导GAT的表达。将培养物继续培养4小时,然后离心收集细胞,并将细胞沉淀保藏在-80℃下。
在0.2g细胞中加入1ml以下缓冲液使细胞裂解:25mM HEPES,pH7.3;100mM
和10%甲醇(HKM),加有0.1mM EDTA、1mM DTT、1mg/ml鸡蛋溶菌酶和蛋白酶抑制剂混合物(cocktail)(得自Sigma,并按制造商的建议使用)。在室温(例如22-25℃)培育20分钟后,短暂超声完成裂解。将溶解产物离心并取上清液,通过HKM平衡的Sephadex G25将上清液脱盐。在CoA琼脂糖(Sigma)上进行亲和层析部分纯化,将此柱用HKM平衡,并在流体静力压下让澄清的提取物通过此柱。通过HKM洗涤除去未结合的蛋白质,并用含1mM辅酶A的HKM洗脱GAT。这一过程提供了4倍的纯化。这时,用粗制的溶解产物上样SDS聚丙烯酰胺凝胶,观察到大约65%的染色蛋白质是GAT,另外的20%是该载体编码的氯霉素乙酰转移酶。
通过Superdex 75(Pharmacia)凝胶过滤部分纯化的蛋白质,实现了均质性纯化。流动相是HKM,其中GAT活性是在相当于分子半径为17kD的体积处洗脱的。取3μg GAT样品在厚1mm的12%丙烯酰胺凝胶上进行SDS聚丙烯酰胺凝胶电泳,考马斯染色判断此物质是均质的。纯化使得此活性提高了6倍。
在含有饱和(200μM)乙酰CoA、不同浓度的草甘膦和1μM纯化的GAT(在含有5mM吗啉的用乙酸和20%乙二醇将pH调至7.7缓冲液中)的反应混合物中,测定了对草甘膦的表现KM。通过在235nm(E=3.4OD/mM/cm)处连续监测乙酰CoA硫酯键的水解,确定初始反应速度。观察到了双曲线样的饱和动力学曲线(图5),由此得到2.9±0.2(SD)的表观KM。
在含有5mM草甘膦、不同浓度的乙酰CoA和0.19μM GAT(含有5mM吗啉的在用乙酸和20%乙二醇将pH调至7.7的缓冲液中)的反应混合物中,测定了对乙酰CoA的表观KM。用质谱测定N-乙酰草甘膦,确定了初始反应速度。在此装置中反复注入5μl反应混合物,并将反应时间对积分的峰面积作图而得到了反应速度(图6)。观察到了双曲线样的饱和动力学曲线(图7),由此得到2μM的表观KM。从已知酶浓度下获得的Vmax值计算出k催化为6/min。
实施例3:质谱(MS)筛检过程
以每个样品26秒的速度从96孔微量滴定板中取出样品(5μl),无需分离,将其注入质谱仪(Micromass Quattro LC,三组四倍器质谱仪)。通过水/甲醇(50∶50)流动相以500UI/min的流速将样品带进质谱仪。通过负电喷射电离方法(针电压-3.5KV;锥电压20V;电子源温度120℃;去溶剂化温度250℃;锥气流90L/Hr;去溶剂化气流600L/Hr),将注入的各个样品离子化。通过第一个四极选择此过程中形成的分子离子(m/z 210),用于在第二个四极中进行碰撞诱导的解离(CID),其中将压力设置为5×10-4毫巴,将碰撞能量调节为20Ev。设定第三个四极用于仅允许从母离子(m/z 210)产生的一个子离子(m/z 124)进入用于记录信号的检测器中。将第一和第三四极设定在单位分辨度并在650V操作光电倍增管。将纯N-乙酰草甘膦标准品用于比较并且使用峰积分来估计浓度。用这种方法可以检测出少于200Nm的N-乙酰草甘膦。
实施例4:天然或低活性GAT酶的检测
天然或低活性的GAT酶的K催化通常约为1min-1,对草甘膦的KM为1.5-10Mm。对乙酰CoA的KM通常小于25μM。
使细菌培养物生长在96孔深孔板的富集培养基中,离心收集0.5ml静止相细胞,用5mM pH8的醋酸吗啉洗涤,并重悬浮在0.1ml含有200μM乙酰CoA铵、5mM草甘膦铵和5μg/ml PMBS(Sigma)的5mM pH8醋酸吗啉反应混合液中。PBMS渗透过细胞膜,允许底物和产物从细胞移至缓冲液而不释放所有细胞成分。在25-37℃下进行反应1-48小时。用等体积的100%乙醇淬灭反应,并用0.45μm的MAHVMultiscreen滤板(Millipore)过滤所有混合物。如上所述用质谱仪分析样品并与合成的N-乙酰草甘膦标准品进行比较。
实施例5:高活性GAT酶的检测
高活性GAT酶的k催化通常最高达400min-1,并且KM通常低于0.1mM草甘膦。
将编码GAT酶的基因克隆入大肠杆菌表达载体pQE80(Qiagen)中,并将其导入大肠杆菌菌株XL1 Blue(Stratagene)。让培养物在浅U底96孔聚苯乙烯平板中的150ul富集培养基(含有50μg/ml羧苄青霉素的LB)中生长至对数后期,并用新鲜的含有1mM IPTG(USB)的培养基作1∶9稀释。培育4-8小时后,收集细胞,用pH 6.8的5mM醋酸吗啉洗涤,重悬于等体积的同样的吗啉缓冲液中。用最多达10ul洗涤过的细胞进行反应。在较高的活性水平,先将细胞最多稀释到1∶200,将5μl加入100μl反应混合物中。为了测量GAT活性,对于低活性,可以使用上述一样的反应混合物。然而,为了测量高活性GAT酶,应将草甘膦浓度降至0.15-0.5mM,pH降至6.8,在37℃下进行反应1小时。反应后处理和MS检测如本文所述。
实施例6:GAT酶的纯化
通过在CoA-琼脂糖上亲和层析细胞裂解物并在Superdex-75上凝胶过滤来进行酶的纯化。用下述方法获得了数量最多达10mg纯化的GAT酶:将100ml在pQE80载体上带有GAT多核苷酸的大肠杆菌培养物在含50μg/ml羧苄青霉素的LB中培养过夜,用于接种1L加有50ug/ml羧苄青霉素的LB。1小时后,加入IPTG至1mM,使培养物再生长6小时。离心收集细胞。将细胞悬浮在25mM HEPES(pH 7.2)、100mM
、10%甲醇(称为HKM)、0.1mM EDTA、1mM DTT、Sigma-Aldrich提供的蛋白酶抑制剂混合物和1mg/ml鸡蛋溶菌酶中进行裂解。在室温下30分钟后,短暂超声细胞。离心除去颗粒物质,使溶解产物通过辅酶A-琼脂糖柱。用几倍柱床体积的HKM洗涤柱,并用1.5倍柱床体积的含有1mM乙酰辅酶A的HKM洗脱GAT。洗脱液中的GAT保留在Centricon YM 50超滤膜上而浓缩。通过一系列0.6ml注射使此蛋白质通过Superdex 75柱而获得进一步纯化。GAT活性峰在相应于分子量为17kD的体积处被洗脱出来。这种方法使得GAT酶被纯化至均一,回收率>85%。用类似方法,每次可以获得0.1-0.4mg含量的最多96个改组的变体。将诱导的培养物的体积减至1-10ml,可在装在MAHV滤板(Millipore)中的0.15ml柱子中进行辅酶A-琼脂糖亲和层析,Superdex 75层析可以省略。
实施例7:测定K催化和KM的标准方法
使用连续的分光光度测定,确定了纯化的蛋白质对草甘膦的K催化和KM,其中在235nm下监测乙酰CoA的硫酯键的水解。在室温(约23℃)下,在96-孔测定板的孔中进行反应,孔中装有最终体积为0.3ml的以下成分:20mM HEPES pH6.8、10%乙二醇、0.2mM乙酰辅酶A和不同浓度的草甘膦铵。比较两种GAT酶的动力学,应该在同等条件下(如都在23℃)测定两种酶。由Vmax和通过Bradford测定确定的酶浓度,计算出K催化。采用Michaelis-Menten方程的Lineweaver-Burke转化,从范围为0.125-10mM的草甘膦浓度获得的初始反应速度计算出KM。通过将测定的K催化的值除以KM的测定值而得到K催化/KM。
使用这种方法,确定了本文例举的大量GAT多肽的动力学参数。例如,用上述试验条件,确定与SEQ ID NO:445相应的GAT多肽的K催
化、KM和K催化/KM分别为322min-1、0.5mM和660mM-1min-1。用上述试验条件已经确定与SEQ ID NO:457相应的GAT多肽的K催化、KM和K
催化/KM分别为118min-1、0.1mM和1184mM-1min-1。用上述试验条件已经确定与SEQ ID NO:300相应的GAT多肽的K催化、KM和K催化/KM分别为296min-1、0.65mM和456mM-1min-1。本领域技术人员可以用这些数字证实,GAT活性试验产生的GAT动力学参数适合与这里所给的数值进行比较。例如,如本文所报告,当条件是用来比较实验GAT和本文例举的GAT多肽时,用于比较GAT活性的条件对于SEQ ID NO:300、445和457应该产生同样的动力学常数(在正常的实验方差内)。
在反应过程中重复取样,用质谱方法测定对乙酰CoA的KM。将乙酰CoA和草甘膦(铵盐)以50×的浓缩储备液添加到质谱样品平板中。加入适当地稀释在挥发性缓冲液(如乙酸盐或碳酸铵,pH 6.8或7.7)中的酶来起始反应。重复地将样品注射到仪器中,从保持时间和峰面积的图形中计算出起始速度。计算对于草甘膦的KM。
实施例8:对转化的大肠杆菌的选择
将进化的GAT基因(具有直接连接到GAT编码序列的5′端上的天然地衣芽孢杆菌核糖体的结合位点(AACTGAAGGAGGAATCTC;SEQ IDNO:515)的嵌合体)克隆到表达载体pQE80(Qiagen)的EcoR I和HindIII位点之间,产生质粒pMAXY2190(图11)。再从质粒中除去His标记结构域,而保留赋予对抗生素氨卡青霉素和羧苄青霉素的抗性的B-内酰胺酶基因。将pMAXY2190电穿孔(BioRad Gene Pulser)到XL1 Blue(Stratagene)大肠杆菌中。将该细胞悬浮在SOC富集培养基中,让其恢复1小时。然后,温和地沉淀细胞,用缺乏芳香氨基酸的M9最小培养基(12.8g/L
、3.0g/L
、0.5g/L
、1.0g/L
、0.4%葡萄糖、2mM
、0.1mM
、10mg/L维生素B,10mg/L脯氨酸、30mg/L羧苄青霉素)洗涤细胞一次,重悬在20ml的相同M9培养基中。以250rpm在37℃下过夜生长后,将等体积的细胞置于M9培养基或者添加了1mM草甘膦的M9培养基中。将无GAT基因的pQE80载体以类似的方式导入大肠杆菌细胞中,铺板获得单一的克隆,用于比较。表3给出对这些结果的总结,GAT活性使得被转化的大肠杆菌细胞以低于1%的背景被选择和生长。表明对于足以使转化细胞生长的GAT活性来说,无需IPTG诱导。通过从在存在草甘膦时生长的大肠杆菌细胞中再分离pMAXY2190而验证了该转化。
表3.在大肠杆菌中的pMAXY2190的草甘膦选择
克隆数量 | ||
质粒 | M9-草甘膦 | M9+1mM草甘膦 |
pMAXY2190 | 568 | 512 |
pQE80 | 324 | 3 |
实施例9:转化的植物细胞的选择
农杆菌介导的植物细胞的转化的效率低。为了繁殖转化的细胞而抑制非转化细胞的繁殖,需要一种可选择的标记。对于卡那霉素和潮霉素的抗生素标记,以及解毒除草剂化合物膦丝菌素的除草剂修饰基因bar,都是可用于植物中的可选择标记的例子(Methods in MolecularBiology,1995,49:9-18)。在此,本发明人证明,GAT活性作为一种有效的植物转化的可选择标记。
将进化的GAT基因(0_5B8)SEQ ID NO:190克隆到植物启动子(增强的草莓叶脉条纹病毒)和泛醌终止子之间,导入到如图12所示的适合于通过根癌农杆菌EHA105转化植物细胞的二元载体pMAXY3793的T-DNA区域中。T-DNA中存在可筛选的GUS标记允许确认转化。使用草甘膦作为唯一的选择试剂来产生转基因的烟草幼芽。
将L.Xanthi烟草的腋芽亚培养在含有蔗糖(1.5%)和脱乙酰吉兰糖胶(0.3%)的半强度MS培养基中,在每2-3周在24℃下16h的光照(35-42μEm-2s-1、冷光荧光灯)下。在2-3周亚培养后,从植物上取下嫩叶,剪切成3×3mm的片段。将根癌农杆菌EHA105接种到LB培养基中,生长过夜到A600=1.0的密度。以4,000rpm离心细胞5分钟,重悬在3×体积的液体共培养培养基中,该培养基由Murashige和Skoog(MS)培养基(pH 5.2)组成,含有2mg/L N6-苄基腺嘌呤(BA)、1%葡萄糖和400μM乙酰丁香酮。然后,将叶子碎片完全浸没在100×25mm Petri培养皿中的20ml的根癌农杆菌中30分钟。用高压灭菌的滤纸印迹,然后,放置到固体的共培养基(0.3%脱乙酰吉兰糖胶)上,如上所述进行培养。在共培养3天后,将20-30个片段转移到基础的诱导生成幼芽的(BSI)培养基中,该培养基由含有2mg/L BA、3%蔗糖、0.3%脱乙酰吉兰糖胶、0-200μM草甘膦和400ug/ml特美汀的MS固体培养基(pH 5.7)组成。
3周后,无论存在或缺乏GAT基因,放置在无草甘膦的培养基中的外植体上明显地生成幼芽。对来自再生幼芽的叶片进行GUS组织化学染色,证明两种构建体都转移了T-DNA。超过20uM的草甘膦浓度完全地抑制任何缺乏GAT基因的外植体形成幼芽。用具有GAT构建体的根癌农杆菌感染的外植体在最高达200uM(最高的检测浓度)的草甘膦浓度下再生幼芽。通过GUS组织化学染色和用退火到启动子和3’区上的引物通过PCR片段扩增GAT基因,来证实转化。结果总结在表4中。
表4.通过草甘膦选择再生烟草幼芽
草甘膦浓度%幼芽再生 | |||||
转移基因 | 0uM | 20uM | 40uM | 80uM | 200uM |
GUS | 100 | 0 | 0 | 0 | 0 |
gat和GUS | 100 | 60 | 30 | 5 | 3 |
实施例10:转化的酵母细胞的草甘膦选择
用于酵母转化的选择标记通常是营养缺陷性基因,使得转化的细胞可以在缺乏特定氨基酸或核苷酸的培养基中生长。由于酿酒酵母对草甘膦敏感,GAT还可以作为一种可选择标记。为了证明这一点,进化的GAT基因(0_6D10),SEQ ID NO:196,从T-DNA载体pMAXY3793克隆得到(如实施例9中所示),作为含有完整编码区域的PstI-ClaI片段,连接到如图13所示的PstI-ClaI消化的p424TEF(Gene,1995,156:119-122)上。该质粒含有大肠杆菌复制起点和赋予羧苄青霉素抗性的基因,以及用于酵母转化的色氨酸营养缺陷型的可选择标记TRP1。
将含有GAT的构建体转化到大肠杆菌XL1 Blue(Statagene)中,铺展在LB羧苄青霉素(50ug/ml)琼脂培养基上。制备质粒DNA,使用转化试剂盒(Biol 01),转化酵母菌株YPH499(Stratagene)。将等量的转化细胞铺展到缺乏所有的芳香氨基酸(色氨酸、酪氨酸和苯丙氨酸)而添加了草甘膦的CSM-YNB-葡萄糖培养基(Biol 01)上。为了比较,也将缺乏GAT基因的p424TEF导入到YPH499在,如上所述进行铺展。该结果证明,GAT活性将可作为一种有效的可选择标记。通过再分离质粒和限制性消化分析,确认在草甘膦选择克隆中存在含有GAT的载体。
实施例11.表达GAT的烟草植物中的除草剂喷洒检测
将如实施例9中所述而生成的烟草幼芽从外植体上切除,转移到基础诱导生根(BRI)培养基中,该培养基由半强度的Murashige和Skoog(MS)培养基,pH 5.7,含有1.5%蔗糖、0.3%脱乙酰吉兰糖胶、0-200uM草甘膦和400ug/ml特美汀。通过切断茎杆并转移到鲜新的BRI培养基中,直到获得期望数量的克隆,无性繁殖生根的植物和腋窝幼芽。小心地从固体培养基中将生根的植物移出。在将植物种植到小罐土壤中之前,洗涤根部以去除任何残留的脱乙酰吉兰糖胶。将保护性的塑料盖保留在植物上至少一周,直到植物已经被充分固定。
为了确定表达GAT的烟草植物是否能够耐受模拟的田间速度的草甘膦喷洒,对各种GAT变体的多个事件的无性系进行检测。典型的检测按照如下进行:对来自各个事件的一个克隆喷洒1ml的草甘膦的异丙胺盐(Sigma P5671)和0.125%Triton X-100的溶液,pH 6.8,使得所喷洒的活性成分的含量等于商业草甘膦产品中的含量。例如,为了获得32盎司/英亩(1X)的含有40%活性成分(″ai″)的除草剂,将2.4ul的40%ai制剂稀释到1ml水中,喷洒到4英寸的正方形罐(16平方英寸)中的植物上。还包括仅使用表面活性剂的模拟应用(OX)。在有些情形下,在1-4周后,进行第二次喷洒。将植物保持在控制的生长房中,在25℃下、70%湿度、16小时光照下。
在本实施例中,10个事件证实GAT0_6D 10(SEQ ID NO:196)的阳性、10个证实GATO_5D3(SEQ ID NO:193)的阳性、8个事件证实rGATO_5B8 SEQ ID NO:190)的阳性,对仅仅用载体(无GAT)转化的植物进行无性繁殖,转移到土壤中,在植物平均具有5片叶子时进行喷洒。也对种子生长的野生型植物进行喷洒。2周后,对仅载体转化的植物和种子生长的植物,用0.5×、2×或4×草甘膦进行喷洒,这些植物停止生长、枯萎和凋零。每一个转基因GAT植物在喷洒过程中都存活了,未出现草甘膦损伤的迹象,例如萎黄病、叶片伸长、矮小或者变棕色(browning)。所有的OX植物都是健康的,包括无GAT的对照植物。3周后,以8×剂量来喷洒所有的存活植物。OX对照植物在两周内死亡。而所有的GAT植物都存活。
用GAT转化的并且在草甘膦上选择的烟草植物都是能够繁育的。开花和结实组与野生型植物无可检测的差别。
实施例12.GAT基因和草甘膦耐受表型的孟德尔遗传学
用转化的拟南芥来证实GAT基因和草甘膦耐受表型的孟德尔遗传学。种植哥伦比亚型拟南芥植物,并且用被称作为嵌合体(SEQ ID NO:16)的GAT变体的构建体,通过浸渍法(Clough,SJ和Bent,AF,(1998)Plant J.16(6):735-43)转化。收集大量种子,用特异于T-DNA中的插入物的引物,通过PCR验证GAT植物。将来自各个事件的T1代种子播种在土壤中,每2英寸的正方形罐中种植10-30粒种子。
当出现第一组真叶时,给罐喷洒等于0.5×和1×商业产品的草甘膦(如实施例11中所计算)。如表5中所示,两周后,转基因和耐受的表型之间的分离是明显的。
表5.0.5×和1×草甘膦耐受的T1拟南芥的分离数据的总结
嵌合体事件(SEQ IDNO:16) | #存活 | #死亡 | 分离比率 |
1 | 8 | 11 | 1∶1.4 |
3 | 6 | 22 | 1∶3.7 |
5 | 26 | 2 | 13∶1 |
13 | 10 | 9 | 1∶1 |
65 | 46 | 19 | 2.4∶1 |
仅载体 | 0 | 22 | - |
野生型 | 0 | 29 | - |
接近3∶1的比率指示一个单一的分离结构域事件。超过3∶1的比率可能是由于小的样本大小的影响、不完全的优势或者位置作用,所引起的表达太低而不足以赋予除草剂耐受性。与对照相比,很明显,GAT基因被传递给T1代,并且赋予草甘膦耐受性。
实施例13:生成表达GAT转基因的草甘膦抗性玉米
用在美国专利5,981,849中描述的方法,生产表达GAT变体转基因的玉米植物,本专利在此引入作为参考。特别地,按照本领域知晓的方法,构建根癌农杆菌载体。各个载体含有具有泛素启动子和内含子、GAT变体和Pin II终止子的插入物。切除未成熟的玉米胚胎,用含有感兴趣的GAT变体的根癌农杆菌载体感染。感染后,转移胚胎,并且培养在共培养基中。共培养后,将被感染的未成熟胚胎转移到含有1.0mM草甘膦(Roundup ULTRA MAXTM)的培养基中。持续该选择,直到鉴定到活跃生长的推定的转基因愈伤组织。取样推定的转基因愈伤组织,用于PCR和Western分析(数据未显示),证实存在GAT基因。将推定的转基因愈伤组织维持在1.0mM草甘膦选择培养基中,以在植物再生前进一步培养和选择。再生时,将被证实为转基因的愈伤组织被转移到含有0.1mM草甘膦的成熟培养基中,培养以使体细胞胚成熟。然后,将成熟胚转移到含有0.1mM草甘膦的再生培养基中,以形成幼芽和根。在出现幼芽和根后,将各个小植物转移到具有生根培养基的试管中,该培养基含有0.1mM草甘膦。将具有生成的幼芽和根的小植物移植到温室内的罐中,以进一步生长,产生T0代的喷洒数据和T1代种子。
为了评价表达GAT变体转基因的转基因玉米植物的草甘膦抗性水平,对温室内的T0代植物喷洒草甘膦(Roundup ULTRA MAXTM)。通过植物变色分值和植物高度的测量值,评价植物抗性水平。按照如下标准,评价植物的变色和植物的高度:
在喷洒草甘膦后第1、2、3和4周时的变色分值
9=无叶/茎变色
7=较小的叶/茎变色
5=更坏的叶/茎变色
3=严重脱色的植物或者变干的植物
1=死亡的植物
植物高度的测量值
在喷洒草甘膦之前
喷洒草甘膦之后的第1、2、3和4周时
成熟的植物(抽穗)
将来自表6所列的各个事件(独立的转基因的愈伤组织)的两株植物送到温室中。植物1被保留来生产种子,不喷洒草甘膦。植物2在移植后第14天被喷洒4×草甘膦(1×草甘膦=26盎司/英亩)。在第7和14天以4×喷洒的T0代植物的变色分值显示在表6和7中。抽穗时的高度数据显示在表14中。进行其他试验,其中对T0代植物喷洒6×草甘膦,在喷洒后10天的数据显示在表8中。
表6.在用4×草甘膦处理后7天时的抗性分值
构建体 | #用4×测试的事件 | 分值为9的事件% | 分值为7的事件% | 分值<7的事件% |
18534(SEQ ID NO:196) | 169 | 30%(50) | 59%(101) | 11%(18) |
18537(SEQ ID NO:193) | 72 | 40%(29) | 54%(39) | 6%(4) |
18540(SEQ ID NO:190) | 111 | 32%(36) | 61%(67) | 7%(8) |
总计 | 352 | 33%(115) | 59%(207) | 8%(30) |
表7.在用4×草甘膦处理后14天时的抗性分值
构建体 | #用4×测试的事件 | 分值为9的事件% |
18534(SEQ ID NO:196) | 169 | 29%(49) |
18537(SEQ ID NO:193) | 72 | 50%(36) |
18540(SEQ ID NO:190) | 111 | 29%(32) |
总计 | 352 | 33%(117) |
表8.在用6×草甘膦处理后10天时的抗性分值
构建体 | #用6×测试的事件 | %在用草甘膦处理后未受损的事件(分值=9) |
19286(SEQ ID NO:814) | 312 | 51%(160) |
19288(SEQ ID NO:549) | 310 | 52%(163) |
19900(SEQ ID NO:738) | 231 | 56%(129) |
19902(SEQ ID NO:638) | 230 | 42%(96) |
21895(SEQ ID NO:848) | 55 | 30%(17) |
21896(SEQ ID NO:912) | 61 | 61%(37) |
21905(SEQ ID NO:906) | 32 | 70%(25) |
总计 | 1231 | 51%(627) |
实施例14:GAT还是一种酰基转移酶
在含有5mM草甘膦或者无草甘膦的反应混合物中,测试GAT变体(B6(SEQ ID NO:7)、0_6D10(SEQ ID NO:448)、17-15H3(SEQID NO:601)和20-8H12c(SEQ ID NO:817))将丙酰基团从丙酰CoA转移到草甘膦的能力。丙酰CoA以1mM的浓度存在。30分钟后,终止反应,通过添加DTNB来确定游离丙酰CoA的存在。所有的变体表现出草甘膦依赖性的对丙酰CoA的水解。这些结果表明,GAT还可以作为一种酰基转移酶。
实施例15:表达GAT转基因的草甘膦抗性的玉米的T1代研究
使用实施例13中描述的方法,制备表达GAT变体转基因18-28D9b(SEQ ID NO:814)和17-15H3(SEQ ID NO:549)的玉米植物。使用T1代植物来生产草甘膦田间耐受数据。对于各个事件,将T1代植物在田间用四种不同的草甘膦喷洒处理(OX、4×、8×和4×+4×)进行处理。以V3和V8对植物进行喷洒。在处理后10天,如实施例13中所描述,对植物的叶片变色和植物高度比较进行评分。T1代的田间喷洒数据与实施例13中报道的先前在温室中获得的结果密切相关。收集T2代种子,用于进一步研究。
实施例16:GAT多肽的热稳定性
A.温度变化对表达GAT转基因的草甘膦抗性植物的草甘膦耐受性的影响
用实施例13中所述的方法,制备表达GAT变体转基因10_4F2(SEQID NO:203)、17-15H3(SEQ ID NO:549)和18-28D9b(SEQ ID NO:814)的玉米植物。在T1代植物中,评价温度对草甘膦耐受性的影响。将T1代植物种植在凉爽/寒冷(白天14℃,夜晚8℃)、温暖(白天28℃,夜晚20℃)和炎热(白天37℃,夜晚20℃)的条件下。用四种不同草甘膦喷洒处理(OX、4×、6×和8×),在V2下对T1代植物进行喷洒。在处理后的第5天和14天,如实施例13中所述,对植物的叶片变色和植物高度比较进行评分。视觉观察表明,草甘膦耐受性并不总是受到所测试的温度范围的负面影响。
B.在体外温度变化对GAT活性的影响
按照如下方法,对多种GAT多肽(DS3(对应于SEQ ID NO:8的天然GAT多肽)、6_6D5(SEQ ID NO:410)、17-15H3(SEQ ID NO:601)、20-8H12(SEQ ID NO:739)、22-13B12(SEQ ID NO:781)和401(对应于SEQ ID NO:6的天然GAT多肽))的体外热稳定性进行评价。将酶分散到200μl条带PCR试管(VWR,圣弗朗西斯科,加利福尼亚州)中,如图17所示,在30℃到60℃之间的各种温度下,在梯度热循环仪器(ML Research,Watertown,MA)中温育15分钟。通过离心去除沉淀的蛋白质,如实施例7中所述,通过连续的分光光度分析,在22℃下,测量剩余的可溶蛋白的仍存在的酶活性。使用饱和浓度的草甘膦(DS3(SEQ ID NO:8)、401(SEQ ID NO:6和6_6D5(SEQ ID NO:410)为10mM;17-15H3(SEQ ID NO:601)、20-8H12(SEQ ID NO:739)和22-13B12(SEQ ID NO:781)为5mM)和AcCoA(167μM)。
在图17中描述了数据。在最高约42-约44℃的温度下,天然(即野生型)的GAT多肽DS3(SEQ ID NO:8)和401(SEQ ID NO:6)的活性是稳定的。对于任何生物体来说非天然的GAT多肽(即非野生型的)在范围约47-约54℃的温度下是稳定的。
按照如下操作,在37.5℃下测量多种GAT多肽的半衰期。将GAT多肽401(SEQ ID NO:6)、17-15H3(SEQ ID NO:601)、20-8H12(SEQ ID NO:739)、22-13B12(SEQ ID NO:781)、22-15B4(SEQID NO:946)和22-18C5(SEQ ID NO:795)温育在25mM Hepes,pH 7.2,10mM
和10%甲醇的基质(“HKM”)中。在各个时间点,取出等分试样,用饱和浓度的草甘膦(401为20mM,剩下的为5mM)和AcCoA(167uM),如实施例7所述,用连续分光光度分析,在22℃下重复分析3次。各个时间点上的标准方差平均为约2.9%。将GAT活性作为温育时间的函数绘图,将数据与指数衰减(y=e-X)的曲线拟合,其中y是酶活性,而x是以小时计算的时间,由此计算出半衰期。数据被显示在下面的表9中。
表9.37.5℃下的GAT多肽的半衰期
酶 | 半衰期,小时 |
401(SEQ ID NO:6) | 14 |
17-15H3(SEQ ID NO:601) | 45 |
20-8H12(SEQ ID NO:739) | 54 |
22-13B12(SEQ ID NO:781) | 67 |
22-15B4(SEQ ID NO:946) | 26 |
22-18C5(SEQ ID NO:795) | 43 |
实施例17:生产表达GAT转基因的草甘膦抗性大豆
用DuPont BiolisticPDS1000/He仪器,采用粒子枪轰击法(参见Klein等人(1987)Nature 327:70-73),生产表达GAT变体转基因的大豆植物。在转化方法中所用的选择试剂为潮霉素。在转基因事件中潮霉素可选择的标记基因保留下来,或者通过本领域知晓的方法切除潮霉素基因。用合成的组成型启动子、GAT变体和Pin II终止子来制备DNA片段。如上所述,将可选择的标记基因,包括35S CaMV启动子、HPT基因和NOS终止子与GAT基因变体一起共轰击。在缺乏选择试剂时,将轰击的大豆胚胎形成悬浮组织培养一周。将胚胎形成悬浮组织在液体选择培养基中放置6周。对推定的转基因悬浮组织取样,用于PCR分析,确定GAT基因的存在。将假定的转基因悬浮培养组织在选择培养基中维持3周,以获得足以用于植物再生的组织。用标准操作,使悬浮组织成熟4周;使成熟的体细胞胚胎干燥4-7天,然后在萌发培养基中放置2-4周。将发芽的植物转移到单元包装盘(cellpack tray)的土壤中3周,以适应环境。将植物栽到温室内的10英寸罐中,评价草甘膦抗性。
为了确定表达GAT变体转基因的转基因大豆的草甘膦抗性水平,在温室中,对T0代的植物喷洒草甘膦(Roundup ULTRA MAXTM)。通过植物变色分值和植物高度测量值,评价植物抗性水平。
喷洒草甘膦后2周时的变色分值
9=无叶子/茎变色
7=较小的叶/茎变色
5=更坏的叶/茎变色
3=严重脱色的植物或者变干的植物
1=死亡植物
对于各个独立的转基因事件,将1-4棵植物送到温室中。将每个事件的另外的1-2棵植物种植在控制环境的生长腔室中,用于种子生产,被喷洒草甘膦。在转移到土壤后的3-4周,对温室植物喷洒1×、2×或者4×草甘膦(1×草甘膦=26盎司/英亩的RoundUp ULTRAMAXTM)。具有2×和4×喷洒速度的T0代的植物的变色分值分别显示在表10和表11中。
这些结果表明,如PCR分析所证明,用GAT基因变体有效地转化了大豆。表达GAT基因变体的转基因大豆抵抗具有2×和4×喷洒速度的草甘膦。在4×草甘膦喷洒速度中存活的事件的确表现出某种程度的较小的叶子变色,但是,在2周的喷洒检测中,植物恢复,并且显示出正常的叶片形态。
表10.用2×草甘膦处理后的第10天的抗性分值
#用2×测试的事件 | 分值为7-8的事件% | 分值为3-6的事件% | |
SEQ ID NO:193 | 27 | 15%(4) | 11%(3) |
SEQ ID NO:824 | 38 | 8%(3) | 74%(23) |
表11.用4×草甘膦处理后的第10天的抗性分值
#用4×测试的事件 | 分值为7-8的事件% | 分值为3-6的事件% | |
SEQ ID NO:824 | 23 | 8%(2) | 43%(10) |
实施例18:盐对GAT动力学的影响
为了更好地接近本发明的GAT酶将被使用的生理学条件(例如植物细胞),在存在所添加的盐时,再次评价一些本发明的GAT酶的活性。对于天然的GAT酶GAT401(SEQ ID NO:6)、B6(SEQ ID NO:7)和DS3(SEQ ID NO:8)和进化的GAT酶06D10(SEQ ID NO:448)、104F2(SEQ ID NO:454)、18-28D9(SEQ ID NO:618)、17-15H3(SEQ ID NO:601)、17-10B3(SEQ ID NO:592)、20-8H12(SEQID NO:739)、20-16A3(SEQ ID NO:639)和20-30C6(SEQ ID NO:683),图15A和15B分别提供了关于动力学参数Km和k催化/Km的比较,在缺乏所添加的
(无阴影栏)或者存在20mM
(阴影栏)下进行分析。用实施例7中所述的Bradford分析,确定蛋白质浓度。由于缺乏
时,这些蛋白质对草甘膦的Km极其低,用实施例3中所述的质谱分析,确定缺乏
时进化GAT酶06D10、18-28D9和20-8H12的动力学参数,而其他动力学参数(无论
存在与否)用实施例7中所述的连续分光光度分析来确定。当可获得时,误差栏表示多个分析的标准偏差。图15A显示,将盐(20mM
)加入到分析缓冲液时,显著地提高对草甘膦的Km值。k催化值仍然相对未改变或者略微升高,最终结果为,存在20mM
时所观察到的对于GAT酶的k催化/Km值低于缺乏所添加的
时的k催化/Km值(图15B)。
实施例19:进一步进化的编码具有非常高活性的GAT酶的GAT基因
额外重复定向的分子进化而获得了进一步进化的GAT基因,其编码具有非常高的GAT活性的GAT酶,例如具有一种或多种改进的特性,如与先前描述的GAT酶相比,降低的对于草甘膦的Km、升高的k催化或者升高的k催化/Km。
首先选择进一步进化的gat基因,用于如实施例8所述将大肠杆菌培养在最小M9培养基中,选择时使用5mM而不是1mM的草甘膦。如上面的实施例6所描述,纯化蛋白质。
在205nm下,通过UV吸光率来确定蛋白质浓度。通过Scopes(1994;Protein Purification,Principles and Practice,Springer,纽约)描述的方法,根据公式E(mg ml-1cm-1)=27+120(A280/A205)=30.5,来确定消光系数。在通过UV吸光率进行量化之前,使用NAP-5柱(Amersham-Pharmacia Biotech),通过缓冲液将蛋白质溶液交换到50mM
中。
示例性的进一步进化的gat编码序列包括在此被确定为SEQ IDNO:832、834、836、838、840、842、844、846、848、850、852、854、856、858、860、862、864、866、868、870、872、874、876、878、880、882、884、886、888、890、892、894、896、898、900、902、904、906、908、910、912、914、916、918、920、922、924、926、928和930的核酸序列,其分别编码包括在此被鉴定为SEQ ID NO:833、835、837、839、841、843、845、847、849、851、853、855、857、859、861、863、865、867、869、871、873、875、877、879、881、883、885、887、889、891、893、895、897、899、901、903、905、907、909、911、913、915、917、919、921、923、925、927、929和931的氨基酸序列的进一步进化的GAT酶。一些这种进一步进化的GAT酶具有非常高的GAT活性,其中其具有一种或多种改进的特性,例如与相同条件下分析的先前描述的GAT酶相比,降低的对于草甘膦的Km、升高的k催化或者升高的k催化/Km。
图16A、16B和16C分别提供了数种先前描述的GAT酶(无阴影栏)的动力学参数Km和k催化/Km与一些本发明的进一步进化的GAT酶(阴影栏)的动力学参数的比较,如上所述,存在20mM
时用连续分光光度分析和通过UV吸光率量化蛋白质来进行分析。当可获得时,误差栏代表多个分析的标准偏差。在这些分析条件下,天然的GAT酶GAT401(SEQ ID NO:6)对于草甘膦的Km为约4mM、k催化为约5.4min-1和k
催化/Km为约1.35mM-1min-1。当在这些条件下进行分析时,一些本发明的进一步进化的GAT酶(阴影栏)对于草甘膦的Km范围小于约0.4mM(例如在约0.4mM和0.1mM之间),k催化值至少为约1000min-1(例如,在约1000min-1和约2500min-1之间),k催化/Km值为至少约4800mM-1min-1(例如,在约4800mM-1min-1和约8000mM-1min-1之间)。例如,在这些分析条件下,一些本发明的进一步进化的GAT酶具有高于天然GAT酶GAT401至少约7000倍的k催化/Km值。
一些本发明的进一步进化的GAT酶包含一个或多个未见于先前描述的GAT多肽和GAT酶上的氨基酸残基,例如在位置27上,存在B1、Z1或A氨基酸残基;在位置33上,存在N或G氨基酸残基;在位置46上,存在B2、Z4或H氨基酸残基;在位置93上,存在R氨基酸残基;其中B1为选自由A、I、L、M、F、W、Y和V组成的组的氨基酸;而B2是选自由R、N、D、C、Q、E、G、H、K、P、S和T组成的组的氨基酸;Z1是选自由A、I、L、M和V组成的组的氨基酸残基;Z4是选自由R、H和K组成的组的氨基酸。例如,一些本发明的进一步进化的GAT酶包含一个或多个:位置27上的Ala(即Ala27);位置33上的Asn或Gly(即Asn33或Gly33);位置46上的His(即His46);和位置93上的Arg(即Arg93),序列编号按照如SEQ ID NO:907的编号。
进行序列/活性分析,确定与高k催化/Km正向相关的氨基酸残基(通过高k催化和低Km或者两者来证明)。看似与高k催化/Km正向相关的氨基酸残基包括Glu14、Asp32、Asn33、Gly38和Thr62(对应于SEQ ID NO:907的序列编号)。可以将一个或多个这种残基的密码子替代到模板GAT多肽的编码序列的适当位置上,来构建其他的GAT酶。例如,将编码位置14的Glu、位置32的Asp、位置33的Asn、位置38的Gly和位置62的Thr的密码子的一个或多个取代到编码模板多肽的核酸序列上,来产生其他GAT酶,模板肽如上述具有非常高活性的进一步进化的GAT酶中的两种,GAT24-5H5(SEQ ID NO:845)或GAT 25-8H7(SEQ ID NO:907)。以这种方式产生的示例性的进一步进化的GAT酶,在此被鉴定为R12G1(SEQ ID NO:917)、R12G2(SEQ ID NO:919)、R12G3(SEQ ID NO:921)、R12G4(SEQ ID NO:923)、R12G5(SEQ ID NO:925)、R12G6(SEQ ID NO:927)、R12G7(SEQ ID NO:929)和R12G8(SEQ ID NO:931),分别由被鉴定为SEQ ID NO:916、918、920、922、924、926、928和930的核酸编码,与模板多肽相比,具有非常高的GAT活性。
实施例20:与高GAT活性相关的氨基酸
氨基酸天冬氨酸(Asp,D)、组氨酸(His,H)和半胱氨酸(Cys,C)与各种乙酰转移酶的活性位点相关是已知的。为了确定任何这些残基是否在GAT活性中起作用,将GAT20-30C6(SEQ ID NO:683)的所有D、C和H残基分别地突变为丙氨酸(Ala,A),分析突变酶对N-乙酰草甘膦的活性。含有取代D34A和H41A的变体仅保留未被修饰的酶的活性的约2%-3%,而含有取代H138A的变体基本上无可检测的GAT活性。另外,含有取代H138R和H138S的变体的GAT活性低,但是可检测到(特别是在高于6.8的pH下),表明位置138上的His(通常为Arg和Ser)可能作为活性位点的基础。
表12
酶 | 0KClBradford蛋白质分析 | |||
K催化(min-1) | Km(mM) | k催化/Km(mM-1min-1) | 20-30C6的k催化/Km% | |
20-30C6(SEQ ID NO:683) | 386 | 0.182 | 2122 | 100 |
20-30C6 H41A | 208 | 4.80 | 43 | 2.0 |
20-30C6 D34A | 127 | 2.33 | 54 | 2.6 |
20-30C6 H138A | <0.02 | Nd | Nd | <0.005 |
20-30C6 H138R | 5.35 | 17.8 | 2.49 | 0.12 |
20-30C6 H138S | 7.1 | 0.75 | 0.03 |
实施例21:改进植物中GAT的表达
植物、动物和微生物具有特定的密码子偏好是已知的,在基因转录本的翻译过程中,影响氨基酸结合的效率。稀有密码子会给翻译过程中的tRNA恢复带来麻烦,然后会导致较低的所编码蛋白的积累。原始的亲本gat基因来自细菌如地衣芽孢杆菌(Bacilluslicheniformis),如此,可能不具有在植物中表达的优化密码子分布。本发明的进化的gat基因已经成功地在植物中表达(参见上面的实施例9、11、13和17),但是仍然存在通过提高在植物中的翻译效率来改进蛋白质生产的可能。实现该目的的一条途径是,将gat编码序列中的一个和多个在植物中很少被使用的密码子替代为在植物中更常用的同一氨基酸的密码子,从而在gat编码序列中产生沉默突变,而不改变所编码蛋白的序列。
比较显示了玉米、棉花和大豆中的密码子使用频率的表格(可从日本千叶的Kazusa DNA Research Institute管理的网站上获得),产生如下表格(表13),显示通常在单子叶或双子叶植物中更经常或较少使用的密码子。
表13
氨基酸 | 植物中较常使用的密码子 | 植物中较少使用的密码子 | |||||||||||
丙氨酸 | Ala | A | GCA | GCC | GCT | GCG | |||||||
半胱氨酸 | Cys | C | TGC | TGT | |||||||||
天冬氨酸 | Asp | D | GAC | GAT | |||||||||
谷氨酸 | Glu | E | GAA | GAG | |||||||||
苯丙氨酸 | Phe | F | TTC | TTT | |||||||||
甘氨酸 | Gly | G | GGA | GGT | GGC | GGG | |||||||
组氨酸 | His | H | CAC | 催化 | |||||||||
异亮氨酸 | Ile | I | ATC | ATT | ATA | ||||||||
赖氨酸 | Lys | K | AAA | AAG | |||||||||
亮氨酸 | Leu | L | TTG | CTC | CTG | CTT | CTA | TTA | |||||
甲硫氨酸 | Met | M | ATG | ||||||||||
天冬酰胺 | Asn | N | AAC | AAT | |||||||||
天冬酰胺 | Asn | N | AAC | AAT | |||||||||
脯氨酸 | Pro | P | CCA | CCT | CCC | CCG | |||||||
谷氨酰胺 | Gln | Q | CAA | CAG | |||||||||
精氨酸 | Arg | R | AGA | AGG | CGA | CGC | CGG | CGT | |||||
丝氨酸 | Ser | S | AGT | TCA | TCC | TCT | AGC | TCG | |||||
苏氨酸 | Thr | T | ACA | ACC | ACT | ||||||||
缬氨酸 | Val | V | GTG | GTT | GTA | GTC | |||||||
色氨酸 | Trp | W | TGG | ||||||||||
酪氨酸 | Tyr | Y | TAC | TAT |
用于提高微生物基因在植物中的表达的第二条途径是,增加接近起始甲硫氨酸残基处的G+C含量。植物中天然存在的编码序列趋向于在紧邻ATG起始密码子的下游含有2个或3个G和/或C残基(Joshi等人(1997)Plant Mol.Biol.35:993-1001)。往gat编码序列中的紧邻ATG起始密码子的下游处导入一个或多个富含CG的密码子,可以产生更加植物样的编码序列,并且因而可能增加其在植物中的表达。将第二个密码子(异亮氨酸,ATA)替代为丙氨酸密码子(GCG),生成与未被修饰的酶相比k催化降低的Ile2Ala变体。另一个方面,将丙氨酸密码子(GCG或GCT)插入到密码子位置1的Met和位置2的Ile密码子之间,生成编码含有插入在位置1的Met和位置2的Ile之间的Ala残基的GAT酶的gat编码序列。含有两个插入在Met1和Ile2之间的丙氨酸的示例性GAT酶变体,鉴定为22-15B4M1MAA(表示在紧接位置1的Met后插入两个Ala残基),具有蛋白质序列SEQ IDNO:948;与未被修饰的酶22-15B4(SEQ ID NO:789)相比,其k催
化下降了。含有插入在Met1和Ile2之间的丙氨酸的示例性GAT酶被称作22-15B4 MIMA(表示在紧接位置1的Met后插入一个Ala残基),具有蛋白质序列SEQ ID NO:946;与未被修饰的酶22-15B4(SEQ IDNO:789)相比,其动力学基本上未改变。
开发了用于改进GAT在植物中的表达的一般策略。例如,根据上述表格,通过将较少在植物中使用的密码子替换为较常在植物中使用的密码子,来改变进化的gat编码序列。较少在植物中使用的密码子(例如,根据上述表格)通常被去除。采取这种方式,gat编码序列中至少一个密码子(例如,至少3个密码子、至少5个密码子或者至少10个密码子)被改变,由植物中较少使用的密码子改变为植物中较常使用的密码子。被替换的密码子可以在gat编码序列的编码序列的5′端(例如,前10个密码子内、前20个密码子内、前50个密码子内或者前100个密码子内)。此外,被替换的密码子可以在整条的gat编码序列上。而且,选择较常使用的密码子,以避免在编码序列中超过约5-10次(例如,超过5次、超过6次、超过7次、超过8次、超过9次、超过10次)的连续出现G+C或A+T。还可以改变编码序列,如在紧邻gat编码序列的下游或者邻近其起始Met密码子处插入一个Ala密码子(例如经常使用的Ala密码子),以在紧邻ATG起始密码子的下游含有一个或多个富含CG的密码子。
表14 提供了如上所述改变的示例性gat编码序列
原始gat编码序列 | 改变的gat编码序列 | 进行的密码子改变 | 所编码的蛋白质 | ||
GAT20-8H12/4604(SEQ ID NO:738) | GAT4604SR(SEQ ID NO:932) | Ser 62 TCG->TCTArg 111 CGG->AGG | 20-8H12(SEQ ID NO:739) | ||
GAT22-18C5/4609(SEQ ID NO:794) | GAT4609SR(SEQ ID NO:933) | Ser 62 TCG->TCTArg 111 CGG->AGG | 22-18C5(SEQ ID NO:795) | ||
GAT22-16D8/4610(SEQ ID NO:792) | GAT4610R(SEQ ID NO:934) | Arg 111 CGG->AGG | 22-16D8(SEQ ID NO:793) | ||
GAT22-15B4/4611(SEQ ID NO:788) | GAT4611R(SEQ ID NO:935) | Arg 111 CGG->AGG | 22-15B4(SEQ ID NO:789) | ||
GAT24-5H5/4614(SEQ ID NO:848) | GAT4614SR(SEQ ID NO:936) | Ser 62 TCG->TCTArg 111 CGG->AGG | 24-5H5(SEQ ID NO:849) | ||
GAT24-5H5/4614(SEQ ID NO:848) | GAT4614VSR(SEQ ID NO:937) | Val 4 GTG->GTASer 62 TCG->TCTArg 111 CGG->AGG | 24-5H5(SEQ ID NO:849) | ||
GAT23-2H11/4615(SEQ ID NO:836) | GAT4615R(SEQ ID NO:938) | Arg 111 CGG->AGG | 23-2H11(SEQ ID NO:837) | ||
GAT24-15C3/4616(SEQ ID NO:862) | GAT4616R(SEQ ID NO:939) | Arg 111 CGG->AGG | 24-15C3(SEQ ID NO:863) | ||
GAT23-6H10/4617(SEQ ID NO:844) | GAT4617R(SEQ ID NO:940) | Arg 111 CGG->AGG | 24-6H10(SEQ ID NO:845) | ||
GAT25-8H7/4618(SEQ ID NO:906) | GAT4618SR(SEQ ID NO:941) | Ser 62 TCG->TCTArg 111 CGG->AGG | 25-8H7(SEQ ID NO:907,957) | ||
GAT25-8H7/4618(SEQ ID NO:906) | GAT4618VSR(SEQ ID NO:942) | Val 4 GTG->GTASer 62 TCG->TCTArg 111 CGG->AGG | 25-8H7(SEQ ID NO:907) | ||
GAT25-19C8/4619(SEQ ID NO:912) | GAT4619SR(SEQ ID NO:943) | Ser 62 TCG->TCTArg 111 CGG->AGG | 25-19C8(SEQ ID NO:913) |
表14(续)
GAT25-19C8/4619(SEQ ID NO:912) | GAT4619VSR(SEQ ID NO:944) | Val 4 GTG->GTASer 62 TCG->TCTArg 111 CGG->AGG | 25-19C8(SEQ ID NO:913) |
GAT22-15B4/4611(SEQ ID NO:788) | GAT4611A(SEQ ID NO:945) | 插入在Met1和Met2之间的Ala | 22-15B4M1MA(SEQ ID NO:946) |
GAT22-15B4/4611(SEQ ID NO:788) | GAT4611AA(SEQ ID NO:947) | 两个插入在Met1和Met2之间的Ala | 22-15B4M1MAA(SEQ ID NO:948) |
GAT25-8H7/4618(SEQ ID NO:906) | GAT4618A(SEQ ID NO:949) | 插入在Met1和Met2之间的Ala | 25-8H7M1MA(SEQ ID NO:950) |
GAT25-8H7/4618(SEQ ID NO:906) | GAT4620(SEQ ID NO:951) | 插入在Met1和Met2之间的Ala和前7个较常使用的密码子;加上Ser 62 TCG->TCTArg 111 CGG->AGG | 25-8H7M1MA(SEQ ID NO:950) |
GAT25-8H7/4618(SEQ ID NO:906) | GAT4621(SEQ ID NO:952) | 插入在Met1和Met2之间的Ala和较常使用的密码子 | 25-8H7M1MA(SEQ ID NO:950) |
通过电穿孔,将在T-DNA中具有dMMV-gat-UBQ3表达盒的双元载体转化到活性根癌农杆菌菌株C58细胞中(McCormac等人,MolBiotechnol.9:155-159,1998)。在28℃下在LB+40ug/ml卡那霉素的平板中生长2天后,将菌落接种到LB+40ug/ml卡那霉素液体培养基中,28℃下摇床培养过夜。以4000g离心10分钟,收集农杆菌细胞,然后重悬在与起始培养体积相当的10mM
中。用1ml塑料注射器(无针头)给细菌悬浮液加压,将其“渗透”到烟草叶片的细胞间隙中。将200-300μl的细菌悬浮液渗透到各个斑点上(通常渗透面积为3-4cm2),在仍然附着在植物上的单个叶片上分布4个或更多个斑点。在某些情形下,在进行渗透之前,用第二种缺乏gat的农杆菌菌株,以5∶1或10∶1稀释含有gat的农杆菌菌株。该稀释步骤具有减少在植物细胞中过度表达gat基因的作用,从而防止饱和,使得更加容易可视化变体和构建体之间的表达差异。3天后,研磨叶片材料,在水溶性缓冲液中进行提取,并离心。上清液含有可溶的蛋白质,进行SDS-PAGE,用抗GAT的多克隆抗体对凝胶进行印迹和探查。
将渗透了GAT4620基因的烟草叶片中积累的GAT蛋白含量与用未被修饰的GAT25-8H7/4618基因转化的叶片中积累的蛋白质的含量进行比较。另一个方面,与表达未被修饰GAT25-8H7/4618基因的叶片相比,具有GAT4621基因的烟草叶片在总蛋白百分含量上具有高两倍的GAT蛋白积累。
实施例22:表达GAT转基因的草甘膦抗性大豆的T1代试验
用实施例17所述方法,制备表达GAT转基因18-28D9c(SEQ ID NO:824)的大豆植物。从喷洒草甘膦的T0代植物收集T1代种子。在温室条件下,T1代种子在从Scotts,Marysville,OH购买的RediEarthR360培养基中萌发,用实施例17所述方法,在V2-V3阶段,喷洒2×或者4×草甘膦(Round Up ULTRAMAXTM,从密苏里州州圣路易斯的孟山都公司购买)。T1代的温室喷洒数据与先前的T0代植物的温室结果十分相关。收集T2代种子,用于进一步研究。
实施例23:生产表达GAT和HRA转基因的草甘膦和磺胺抗性的大豆
用实施例17的方法,制备表达GAT & HRA的大豆植物,hRA是乙酰乳酸合酶的高抗性等位基因(美国专利5,605,011、5,378,824、5,141,870和5,013,659)。HRA基因用作转化的可选择标记基因。选择试剂为浓度为100ng/ml的氨磺隆。可选择标记基因由来自大豆(Glycine max)的S-腺苷-L-甲硫氨酸合成酶(SAMS)启动子(U.S.2003/226166)、来自大豆的HRA编码序列和来自大豆的乙酰乳酸合酶终止子组成。可选择的标记基因被连接到GAT构建体上,或者与该构建体共轰击,该构建体由合成的组成型启动子(美国专利6,072,050和6,555,673)或者玉米组蛋白2B启动子(美国专利6,177,611)、GAT变体(18-28D9c(SEQ ID NO:824))和Pin II终止子(Gyheung an等人,Plant Cell 1:115:122(1989))组成。如实施例17所述,产生转基因植物。如实施例17所述,在2×或4×草甘膦应用速度后,用植物变色分值确定草甘膦抗性水平。显示在表15中的结果证明,不同的组成型启动子驱动GAT变体(18-28D9c(SEQ IDNO:824)),在T0植物中赋予草甘膦抗性。
表15.用4×草甘膦处理后10天的抗性分值
事件 | 用4×检测的#事件 | 7-8分值的事件% | 3-6分值的事件% |
PHP20163a(45)(SEQ ID NO:824)(SCP1启动子) | 58 | 15.5%(9) | 77.6%(45) |
PHP20558a(11)(SEQ ID NO:824)(H2B启动子) | 26 | 34.6%(9) | 42.3%(11) |
实施例24:表达GAT和HAR转基因的大豆的T1代出土前试验
将从如实施例17所述的试验中产生的T1种子种植在温室内的一盆Tama Silt loam中。以70gms活性成分/公顷的速度,立即对盆喷洒出土前施用的氨嘧磺隆(chlorimuron)、玉嘧磺隆(rimsulfuron)或者苯磺隆(tribenuron)。在喷洒施用后的第10天,按照实施例17所述的植物变色分值,对萌发的植物进行评价。所有的HAR和GAT事件以9级(未受损),在所有的出土前喷洒应用中存活。这些结果证明,大豆中具有对磺胺的出土前抗性。
实施例25:表达GAT和HAR转基因的大豆的T1代出土后试验
在温室内,从如实施例17所述的试验中产生的T1种子在RediEarthR360培养基中萌发。在V2-V3阶段(种植后14天),对植物喷洒噻磺隆(thifensulfuron)、豆磺隆、玉嘧磺隆或苯磺隆(分别为70、70、35、35gm a.i./公顷)。在喷洒施用后的第10天,按照实施例17所述的植物变色分值,对植物进行评价。结果示于表16中。
表16.用磺胺进行出土后处理后第10天的抗性分值
GAT(SEQ ID NO:824)/SAMS启动子-HRA事件的平均抗性分值 | |
未喷洒的对照 | 9 |
氯嘧磺隆(70gm活性成分/公顷) | 7.75 |
玉嘧磺隆(35gm活性成分/公顷) | 2.21 |
苯磺隆(35gm活性成分/公顷) | 3.83 |
噻磺隆(70gm活性成分/公顷) | 7.81 |
如实施例17所述的各种方法,10天后,对用噻磺隆喷洒后具有7级或8级的植物变色的事件喷洒2×或4×的草甘膦用量。按照实施例17所述的变色分值,对事件进行评价。所有的噻磺隆耐受事件以7级或8级在草甘膦喷洒中存活(数据未显示)。这些结果证明,在温室条件下,分别由HRA和GAT基因赋予的噻磺隆耐受性和草甘膦耐受性是100%相关,分别耐受70gm活性组分/公顷噻磺隆和2×草甘膦。
实施例26:表达GAT转基因的草甘膦抗性玉米植物的T3代试验
采用实施例13中描述的方法,制备表达GAT转基因20-H812(SEQID NO:738)和20-16A3(SEQ ID NO:638)的草甘膦抗性玉米植物。10天后对植物评分,并如实施例13所述确定叶片变色分值。特别地,在V4叶片阶段对植物进行喷洒。在进行喷洒处理后,在等间距内的植物变得稀少,竖立的数量也变少。购买得到的NK603(孟山都,圣路易斯,密苏里州州)作为对照。抗性分值显示在表18中。处理后第10天,进行植物高度的测量,结果显示在表18中。
表18.用草甘膦处理后第10天的抗性分值
构建体 | 抗性分值(1-9级) | ||||
测试事件 | 无草甘膦处理的对照 | 1×(26盎司/A)UltraMax | 4×(104盎司/A)UltraMax | 8×(208盎司/A)UltraMax | |
19900(SEQ ID NO:738) | 2 | 9 | 8.8 | 7.9 | 5.4 |
19902(SEQ ID NO:638) | 4 | 9 | 8.4 | 8.0 | 5.9 |
NK603 | 1 | 9 | 8.4 | 7.9 | 5.5 |
表19.用草甘膦处理后第10天的植物高度(英寸)
构建体 | 抗性分值(1-9级) | ||||
测试事件 | 无草甘膦处理的对照 | 1×(26盎司/A)UltraMax | 4×(104盎司/A)UltraMax | 8×(208盎司/A)UltraMax | |
19900(SEQ ID NO:738) | 2 | 18.5 | 16.6 | 16.4 | 16.2 |
19902(SEQ ID NO:638) | 4 | 19.5 | 16.7 | 17.0 | 16.2 |
NK603 | 1 | 20.1 | 17.6 | 17.5 | 17.3 |
实施例27:表达GAT转基因的草甘膦抗性玉米的T3代的产量试验
用从实施例15获得的T3代种子生产T3代植物,用于产生关于杂合体的草甘膦田间耐受性数据。该试验在智利的Viluco进行,采用裂区(split-plot)设计,四个(4)重复。特别地,包括3个试验对象(entries)。这些试验对象中的两个包括表达GAT变体转基因17-15H3(SEQ ID NO:549)的玉米植物。草甘膦抗性对照Nk603可以从孟山都购买得到,作为第三个试验对象。对于每个事件,将所有的试验对象在田间用四种不同的草甘膦喷洒处理(OX、在V4阶段以4×,在V4阶段以8×,和在V4阶段以4×和在V8阶段以4×)进行处理。处理后第10天,如实施例13所述对植物的高度比较进行评分。T3代田间喷洒数据与实施例15中报道的先前在田间获得的结果十分相关。特别地,所有以1×和4×草甘膦喷洒的试验对象在高度上与未喷洒的对照相似。在较高的V4以4×和V8以4×喷洒速度时,GAT对象的高度暂时地下降了12-17%,而NK603下降了6%;但是在季节(在繁殖器官成熟过程中),用草甘膦处理的对象的高度与未喷洒的对象的高度相同。而且,草甘膦处理的对象的产量在数量上和统计学上并不低于未被喷洒的对象(LSD0.05=11.8bu./英亩,每个对象的平均产量=243bu./英亩)。种植在Johnston,IA和York,NE的初步试验田中的相同事件的T2植物,也获得类似结果(数据未显示)。
实施例28:表达GAT转基因的草甘膦抗性玉米的T2代试验
对GAT阳性和GAT阴性的不同品系进行试验。采用实施例17所述的方法,制备表达GAT转基因18-28D9b(SEQ ID NO:814)、17-15H3(SEQ ID NO:549)、20-8H12(SEQ ID NO:738)、20-16A3(SEQID NO:638)的玉米植物。对T2代植物进行检查。在V4阶段,以1×(26盎司/A UltraMaxTM)喷洒GAT阳性的T2代植物。在V4阶段,对GAT阴性的T2代植物进行PCR取样。从每一行中去除GAT阳性植物。不对GAT阴性植物应用草甘膦。抽取植物,使得每一行中植物间的间距相等。进行4个(4)重复。对从每一行的中央收获的5条(5)穗上获得的谷物进行干燥和称重。如表20中所示,在任何构建体中,都未检测到产量下降。
表20.产量数据
构建体 | #事件 | 产量在V阶段,喷洒1×(26盎司/英亩)ULTRA MAXTM的GAT阳性 | 产量未施用草甘膦的GAT阴性的 |
PHP19286(SEQ ID NO:814) | 40 | 1.65lbs/5穗 | 1.57lbs/5穗 |
PHP19288(SEQ ID NO:549) | 40 | 1.64lbs/5穗 | 1.60lbs/5穗 |
PHP19900(SEQ ID NO:738) | 6 | 1.20lbs/5穗 | 1.23lbs/5穗 |
PHP19902(SEQ ID NO:638) | 4 | 1.19lbs/5穗 | 1.21lbs/5穗 |
实施例29:GAT多肽的氨基酸底物
用许多氨基酸底物,对数种本发明的GAT多肽的GAT活性进行评价。将GAT多肽、AcCoA和氨基酸底物温育在96孔聚苯乙烯平板的孔中的25mM Hepes,pH 6.8,10%乙二醇中。30分钟后,加入30μl溶于500mM Tris,pH 7.5中的10mM 5,5′-二硫代双-2-硝基苯甲酸(DTNB)来终止反应。2分钟后,在412nm下,在Spectramax Plus平板阅读器(Molecular Devices,Sunnyvale,加利福尼亚州)读取吸光率。
除了草甘膦,天然的GAT多肽401(SEQ ID NO:6)(或者在为膦丝菌素的情形下为B6(SEQ ID NO:7))对于12种氨基酸具有可检测的活性。天然的GAT多肽对L-天冬酰胺的活性约与草甘膦一样,而对L-丝氨酸的活性比草甘膦约高4.7倍,对磷酰-L-丝氨酸的活性比草甘膦约高2倍。当与天然的GAT多肽比较时,非天然的GAT多肽17-15H3(SEQ ID NO:601)和25-8H7(SEQ ID NO:907)对天冬酰胺的活性高40倍,但是对丝氨酸和膦丝菌素的活性失去了。
除了天冬酰胺和丝氨酸外,对于如下的L-氨基酸,天然GAT多肽的活性为对1mM浓度存在的草甘膦的活性的3%或更高:组氨酸(10%)、酪氨酸(18%)、色氨酸(250%)、缬氨酸(12%)、谷氨酸酰胺(51%)、天冬酰胺(27%)、谷氨酸(32%)、丙氨酸(33%)、甘氨酸(21%)和半胱氨酸(50%)。对其他蛋白质氨基酸的活性不可检测或者低于对作为底物的草甘膦的GAT活性的3%。对于L-天冬酰胺(2mM)、L-丙氨酸(10mM)和甘氨酸的N-甲基衍生物(即肌氨酸,10mM),天然的GAT多肽未观察到可检测的活性。该百分率是相对于GAT多肽对于底物草甘膦的活性的活性百分数。一些数据显示在表21中。
表21.对于草甘膦、天冬酰胺和丝氨酸的GAT活性
GAT变体 | 底物 | K催化±SEmin-1 | KM±SEmM | K催化/KMmin-1mM-1 | K催化/KM |
401 | 草甘膦 | 5.35±0.043 | 1.27±0.0144 | 4.21 | 改善的倍数 |
17-15H3 | 1150±27.6 | 0.251±0.0041 | 4573 | 1086 | |
25-8H7 | 1480±65.4 | 0.05 | 29600 | 7031 | |
%草甘膦 | |||||
401 | 天冬酰胺 | 24.1 | 6.7 | 3.6 | 85.5 |
17-15H3 | 435±11.3 | 2.95±0.162 | 148 | 3.24 | |
25-8H7 | 702±12.4 | 4.56±0.112 | 154 | 0.520 | |
401 | 丝氨酸 | 854 | 43 | 19.8 | 471 |
17-15H3 | 242±15.8 | 60.1±1.68 | 4.04 | 0.0882 | |
25-8H7 | 388±18.5 | 154±10.7 | 2.53 | 0.00855 |
实施例30:pH对GAT活性的影响
用实施例7中所述的分光光度分析方法,除了分析用的缓冲液为滴定到一定的pH值范围的50mM Hepes和10%乙二醇外,确定对于野生型酶B6(SEQ ID NO:7)和GAT多肽17-15H3(SEQ ID NO:601)的k催化和KM的pH最佳值。通过实施例19中描述的UV吸光率分析方法,确定蛋白质浓度。对于克隆B6(SEQ ID NO:7)和GAT多肽17-15H3(SEQ ID NO:601),pH对k催化和KM的影响显示在图18中。
虽然出于清楚和理解的目的,前述的发明已经被略微详细地描述,本领域技术人员应当清楚,通过阅读本公开,可以从形式上和详细地进行各种改变,而不脱离本发明的实际保护范围。例如,上述的所有技术、方法、组合物、设备和系统可以各种组合来使用。本发明旨在包括所有在此描述的方法和试剂,以及所有的多核苷酸、多肽、细胞、生物体、植物、农作物等,它们是这些新方法和试剂的产物。
所有的出版物、专利、专利申请或者其他在本申请中引用的文件处于所有目的被完全引入作为参考,与每个单独的出版物、专利、专利申请或者其他文件出于各种目的被个别地说明引入作为参考的程度相同。
序列表
<110>Castle,Linda A.
Siehl,Daniel
Giver,Lorraine
Minshull,Jeremy
Ivy,Christina
Chen,Yong Hong
Patten,Phillip
Gordon,Rebecca
Duck,Nicholas B.
McCutchen,Billy Fred
Kemble,Roger
<120>新的草甘膦-N-乙酰转移酶(GAT)基因
<130>035718/277848
<140>
<141>2004-04-29
<150>US 10/427,692
<151>2003-04-30
<150>CL 863-2003
<151>2003-04-29
<160>972
<170>FastSEQ for Windows Version 4.0
<210>1
<211>441
<212>DNA
<213>地衣芽胞杆菌
<220>
<223>ST401 gat
<400>1
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcgg gggtgcgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cttcctttca taaagccgaa 180
cattcagagc ttgaaggcga agaacagtat cagctgagag ggatggcgac gcttgaagga 240
taccgtgagc aaaaagcggg aagcacgctc atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaatgcc aggacatctg tgagcggcta ctatgaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgatcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>2
<211>441
<212>DNA
<213>未知的
<220>
<223>从土壤样品得到的未鉴定的微生物
<220>
<223>B6 gat
<400>2
atgattgaag tcaaacctat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggcg gatattatcg ggacaggctg atcagcatcg cctcctttca tcaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaaggg 240
taccgcgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcag accttttatg gtgcaacgcc aggacatctg tgagcgggta ctataaaaag 360
ctcggcttca gcgaacaagg cggggtctac gatataccgc cgatcggacc tcatattttg 420
atgtataaga aattgacata a 441
<210>3
<211>441
<212>DNA
<213>未知的
<220>
<223>从土壤样品得到的未鉴定的微生物
<220>
<223>DS3
<400>3
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcgg gggtacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cttcctttca taatgccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac gcttgaaggg 240
taccgcgagc aaaaagcggg aagtacgctt atccgccatg ccgaagagct tcttcgaaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggatatctg tgagcggcta ctatgaaaag 360
ctcggcttca gcgaacaagg cgggatctac gacataccgc cgatcggacc tcatattttg 420
atgtataaga aattggcata a 441
<210>4
<211>441
<212>DNA
<213>未知的
<220>
<223>从土壤样品得到的未鉴定的微生物
<220>
<223>NHA-2
<400>4
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcgg gggtacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cttcctttca taatgccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac gcttgaaggg 240
taccgcgagc aaaaagcggg aagtacgctt atccgccatg ccgaagagct tcttcgaaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggatatctg tgagcggcta ctatgaaaag 360
ctcggcctca gcgaacaagg cgggatctac gacataccgc cgatcggacc tcatattttg 420
atgtataaga aattggcata a 441
<210>5
<211>441
<212>DNA
<213>未知的
<220>
<223>从土壤样品得到的未鉴定的微生物
<220>
<223>NH5-2
<400>5
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcgg gggtgcgttt 120
cacctcggtg gatattacca gggcaagctg atcagcatcg cttcctttca taaagccgaa 180
cattcagagc ttgagggcga agaacagtat cagctgagag ggatggcgac gcttgaagga 240
taccgtgagc aaaaagcggg aagcacgctc atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaatgcc aggacatctg tgagcggcta ctatgaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgatcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>6
<211>146
<212>PRT
<213>地衣芽胞杆菌
<220>
<223>ST401 GAT
<400>6
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Lys Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Glu Glu Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Val Ser Gly Tyr Tyr Glu Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>7
<211>146
<212>PRT
<213>未知的
<220>
<223>从土壤样品得到的未鉴定的微生物
<220>
<223>B6 GAT
<400>7
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Asp
35 40 45
Arg Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Val Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Gly
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>8
<211>146
<212>PRT
<213>未知的
<220>
<223>从土壤样品得到的未鉴定的微生物
<220>
<223>DS3 GAT
<400>8
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Asn Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Ile
100 105 110
Ser Val Ser Gly Tyr Tyr Glu Lys Leu Gly Phe Ser Glu Gln Gly Gly
115 120 125
Ile Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Ala
145
<210>9
<211>146
<212>PRT
<213>未知的
<220>
<223>从土壤样品得到的未鉴定的微生物
<220>
<223>NHA-2 GAT
<400>9
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Asn Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Ile
100 105 110
Ser Val Ser Gly Tyr Tyr Glu Lys Leu Gly Leu Ser Glu Gln Gly Gly
115 120 125
Ile Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Ala
145
<210>10
<211>146
<212>PRT
<213>未知的
<220>
<223>从土壤样品得到的未鉴定的微生物
<220>
<223>NH5-2 GAT
<400>10
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Ala Phe His Leu Gly Gly Tyr Tyr Gln Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Lys Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Glu Glu Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Val Ser Gly Tyr Tyr Glu Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>11
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>13_10F6
<400>11
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcag gggtacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac actcgaagga 240
taccgtgagc aaaaagcggg aagcacgctc atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggacgtctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>12
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>13_12G6
<400>12
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcag gggtgcgttt 120
cacctcggtg gatattaccg gggcaagctg gtcagcatcg cctcctttca tcaagccgaa 180
catccagagc ttgaaggcca aagacagtat cagctgagag ggatggcgac acttgaaggg 240
taccgtgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag acctcttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgactgggcc ccatattttg 420
atgtataaga aattgacata a 441
<210>13
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>14_2A5
<400>13
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gagcacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cttcctttaa tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaaggg 240
taccgtgagc aaaaagcggg aagcacgctt atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggacgtctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacacaccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>14
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>14_2C1
<400>14
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcag gggtgcgttt 120
cacctcggtg gatattaccg gggcaagctg gtcagcatcg cttcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac actcgaagga 240
taccgtgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacacaccgc cgactgggcc ccatattttg 420
atgtataaga aattgacgta a 441
<210>15
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>14_2F11
<400>15
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcag gggtgcgttt 120
caccttggtg gatattaccg gggcaagctg gtcagcatcg cctcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac actcgaagga 240
taccgtgagc aaaaagcggg cagtacgctt atccgccatg ccgaagcgct tcttcggaaa 300
aagggggcag acctcttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacacaccgc cggccggacc ccatattttg 420
atgtataaga aattgacgta a 441
<210>16
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>嵌合体
<400>16
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcag gggtgcgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cttcctttca tcaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagga 240
taccgcgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacacaccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>17
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>10_12D7
<220>
<221>混杂的特征
<222>54
<223>n=A,T,C或G
<400>17
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgnattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gggcacgctt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cttcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagag 240
taccgcgagc aaaaagcggg aagcacgctc atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag acctcttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaagg cgaagtctac gacataccgc cgaccggacc ccatattttg 420
atgtataaga aattgacgta a 441
<210>18
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>10_15F4
<400>18
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcag gggtacgttt 120
cacctcggtg ggtattaccg gggcaagctg gtcagcatcg cttcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagag 240
taccgcgagc aaaaagcggg aagcacgctc atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaagg cggggtctac gacataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>19
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>10_17D1
<400>19
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cttcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaaggg 240
taccgcgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggcgcag accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaagg cgaagtctac gacacaccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>20
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>10_17F6
<400>20
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggtg gatattaccg gggcaagctg gtcagcatcg cttcctttca tcaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagag 240
taccgcgagc aaaaagcggg aagcacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggcgcag accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>21
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>10_18G9
<400>21
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaactg atttgctcgg tggcacgttt 120
cacctcggtg gatattaccg gggcaagctg gtcagcatcg cttcctttca tcaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagag 240
taccgcgagc aaaaagcggg aagcacgctc atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag acctcttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaagg cggggtctac gacataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>22
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>10_1H3
<400>22
atgattgaag tcaaaccaat aaacgcggag gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gggtacgttt 120
cacctcggtg gatattatcg gggcaagctg gtcagcatcg cttcctttca tcaagccgaa 180
catccagagc ttgaaggccg aaaacagtat cagctgagag ggatggcgac acttgaaggg 240
taccgcgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgaccggacc ccatattttg 420
atgtataaga aattgacata a 441
<210>23
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>10_20D10
<400>23
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcgg gggcacgctt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagag 240
taccgcgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacatctg cgagcggcta ctataaaaag 360
ctcggcttca gcgaacaagg cggggtctac gacataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>24
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>10_23F2
<400>24
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcatactc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggtg gatattaccg gggcaagctg gtcagcatcg cttcctttca tcaagccgaa 180
cacccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac actcgaagga 240
taccgtgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag acctcttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacacaccgc cggtcggacc tcatattttg 420
atgtataaga aat tgacgta a 441
<210>25
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>10_2B8
<400>25
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gggtacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagag 240
taccgcgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag acctcttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacacaccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>26
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>10_2C7
<400>26
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcag gggtgcgttt 120
cacctcggcg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac actcgaaggg 240
taccgtgagc aaaaagcggg aagcacgctc atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacacaccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>27
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>10_3G5
<400>27
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggtg gatattaccg gggcaagctg gtcagcatcg cttcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaaggg 240
taccgcgagc aaaaagcggg aagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgaccggacc ccatattttg 420
atgtataaga aattgacgta a 441
<210>28
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>10_4H7
<400>28
atgattgaag tcaaaccgat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggtg gatattaccg gggcaagctg gtcagcatcg cttcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaaggg 240
taccgtgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgaccggacc ccatattttg 420
atgtataaga aattgacgta a 441
<210>29
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>10_6D11
<400>29
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gggcacgctt 120
cacctcggtg gatattaccg gggcaagctg gtcagcatcg cttcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac gcttgaaggg 240
taccgtgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>30
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>10_8C6
<400>30
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gggtgcgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac actcgaagga 240
taccgcgagc aaaaagcggg aagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaagg cggggtctac gacataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>31
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>11C3
<400>31
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgcaag tatgaaaccg atttgctcgg gggtacgttt 120
cacctcggtg gatattacca gggcaagctg atcagcatcg cctcctttta tcaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac gcttgaaggg 240
taccgcgagc aaaaagcggg aagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacatctg tgagcgggta ctataaaaag 360
ctcggcttca gcgaacaagg cggggtctac gatataccgc cgatcggacc tcatattttg 420
atgtataaga aattgacata a 441
<210>32
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>11G3
<400>32
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcgtgtatg tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggcg gatattacca gggcaagctg atcagcatcg cttcctttca tcaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac gcttgaaggg 240
taccgcgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacatctg cgagcggcta ctatgaaaag 360
ctcggcttca gcgaacaagg cggggtctac gatataccgc cgatcggacc tcatattttg 420
atgtataaga aattggcata a 441
<210>33
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>11H3
<400>33
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcatactc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcag gggtgcgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
cacccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaaggg 240
taccgtgagc aaaaagcggg cagtacgctt atccgccatg ccgaagcgct tcttcggaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttta gcgaacaggg cgaagtctac gacataccgc caactgggcc ccatattttg 420
atgtataaga aattgacgta a 441
<210>34
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>12_1F9
<400>34
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcatactc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaaggg 240
taccgcgagc aaaaagcggg aagcacgctc atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtccac gacataccgc cgaccggacc ccatattttg 420
atgtataaga aattgacgta a 441
<210>35
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>12_2G9
<400>35
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaat cagccgctgga agcatgcaag tatgaaaccg atttgctcgg gggtacgttt 120
cacctcggcg gatattaccg gggcaagctg gtcagcatcg cttcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagga 240
taccgtgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag acctcttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacacaccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>36
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>12_3F1
<400>36
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgcttgg gggcacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cttcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac actcgaagga 240
taccgtgagc aaaaagcggg aagtacgctc atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaagg cggggtctac gacataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>37
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>12_5C10
<400>37
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggtg gatattatcg gggcaagctg atcagcatcg cttcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagag 240
taccgcgagc aaaaagcggg aagcacgctc atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacgcaccgc cgaccggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>38
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>12_6A10
<400>38
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggcg gatattaccg gggcaagctg gtcagcatcg cctcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagga 240
taccgtgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaagg cggggtctac gacataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>39
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>12_6D1
<400>39
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgtatg tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cttcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagag 240
taccgcgagc aaaaagcggg aagcacgctc atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaagg cggggtctac gacataccgc ctgtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>40
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>12_6F9
<400>40
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgtaag tatgaaaccg atttgctcgg gggtacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac actcgaagga 240
taccgcgagc aaaaagcggg aagcacgctc atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacatctg cgagcggcta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgaccggacc ccatattttg 420
atgtataaga aattgacgta a 441
<210>41
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>12_6H6
<400>41
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcatactc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggtg gatattaccg gggcaagctg gtcagcatcg cctcctttca ccaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaaggg 240
taccgtgagc aaaaagcggg cagtacgctt atccgccatg ccgaagcgct tcttcggaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgaccggacc ccatattttg 420
atgtataaga aattgacata a 441
<210>42
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>12_7D6
<400>42
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaactg atttgctcgg gggcacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cttcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaaggg 240
taccgcgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaagg cggggtctac gacataccgc cgaccggacc ccatattttg 420
atgtataaga aattgacgta a 441
<210>43
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>12_7G11
<400>43
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagga 240
taccgcgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacacaccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>44
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>12F5
<400>44
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcgg gggtacgttt 120
cacctcggtg gatattacca gggcaagctg atcagcatcg cttcctttca taaagccgaa 180
cattcagagc ttgagggcca aaaacagtat cagctgagag ggatggcgac acttgaaggg 240
taccgcgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaatgcc aggacatctg tgagcgggta ctataaaaag 360
ctcggcttca gcgaacaagg cgggatctac gacataccgc cgatcggacc tcatattttg 420
atgtataaga aat tgacgta a 441
<210>45
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>12G7
<400>45
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgcaag tatgaaaccg atttgctcgg gggtacgttt 120
cacctcggtg gatattacca gggcaagctg atcagcatcg cttcctttca taaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac gcttgaagga 240
taccgtgagc aaaaagcggg aagcacactc atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcag accttttatg gtgcaacgcc aggacatctg tgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgatcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>46
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>1_2H6
<400>46
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgtatg tatgaaaccg atttgctcgg gggtgcgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaaggg 240
taccgcgagc aaaaagcggg aagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaagg cggggtctac gacataccgc cgatcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>47
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>13_12G12
<400>47
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcgg gggtacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cttcctttaa tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagag 240
taccgcgagc aaaaagcggg aagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cggtcggacc tcatattttg 420
atgcataaga aattgacgta a 441
<210>48
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>13_6D10
<400>48
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg attcgctcgg aggcacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cttcctttaa tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac actcgaaggg 240
taccgtgagc aaaaagcggg aagcacgctc atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag acctcttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacacaccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>49
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>13_7A7
<400>49
atgatcgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcag gagtgcgttt 120
cacctcggcg gatattaccg gggcaagctg atcagcatcg cctcctttca ccaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgaggg ggatggcgac acttgaagag 240
taccgcgagc aaaaagcggg aagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacacaccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>50
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>13_7B12
<400>50
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gagcacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac actcgaagga 240
taccgcgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttgtg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgactgggcc ccatattttg 420
atgtataaga agttgacgta a 441
<210>51
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>13_7C1
<400>51
atgattgaag tcaaaccaat aaatgcggaa gatacgtatg agatcaggca ccgcatactc 60
cggccgaatc agccgcttga agcatgcaag tatgaaaccg atttgctcag gggtgcgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
catccagaac ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagga 240
taccgtgagc aaaaagcggg tagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttgtg gtgcaacgcc aggacatctg cgagagggta ctataaaaag 360
ctcggcttca gcgaacaagg cgaagtctac gacataccgc cgactgggcc ccatattttg 420
atgtataaga aattgacgta a 441
<210>52
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>13_8G6
<400>52
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg attcgctcgg gggcacgttt 120
cacctcggcg gatattaccg gggcaagctg atcagcatcg cttcctttaa tcaagccgaa 180
catccagagc ttgaaggtca aaaacagtat cagctgagag ggatggcgac acttgaagga 240
taccgtgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggacgtctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaagg cggggtctac gacataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>53
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>13_9F6
<400>53
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atctgcttgg gggcacgttt 120
cacctaggtg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagag 240
taccgcgagc aaaaagcggg aagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>54
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>14_10C9
<400>54
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcatactc 60
cggccgaatc agccgctaga agcatgcaag tatgaaaccg atttgctcag gggtgcgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cttcctttca tcaagctgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagag 240
taccgcgagc aaaaagcggg aagtacgctc atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacgtctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacacaccgc cggtcggacc tcatattttg 420
atgtataaga agttgacgta a 441
<210>55
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>14_10H3
<400>55
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcag gggtgcgttt 120
cacctcggcg gatattaccg gggcaagctg gtcagcatcg cctcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagag 240
taccgcgagc aaaaagcggg aagcacgctc atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcag accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacacaccgc cggtcggacc tcatattttg 420
atgtataaga agttgacgta a 441
<210>56
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>14_10H9
<400>56
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcatactc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcag gggtgcgttt 120
cacctcggtg gatattaccg gggcaagctg gtcagcatcg cctcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagga 240
taccgtgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttgtg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacacaccgc cggtcggacc tcatattttg 420
atgtataaga aattgacata a 441
<210>57
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>14_11C2
<400>57
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gagcacgttt 120
cacctcggcg gatattaccg gggcaagctg gtcagcatcg cttcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagag 240
taccgcgagc aaaaagcggg cagtacgctt atccgccatg ccgaagcgct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacacaccgc cgaccggacc ccatattttg 420
atgtataaga aattgacgta a 441
<210>58
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>14_12D8
<400>58
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgtaag tatgaaaccg atttgctcgg gggtacgttt 120
cacctcggcg gatattaccg gggcaagctg gtcagcatcg cctcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagga 240
taccgtgagc aaaaagctgg cagtacgctt atccgccatg ccgaagcgct tcttcggaaa 300
aaaggcgcgg accttttgtg gtgcaacgcc aggacatctg cgagcggcta ctataaaaag 360
ctcggcttca gggaacaagg cggggtctac gacataccgc ctgtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>59
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>14_12H6
<400>59
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gggtgcgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagag 240
taccgcgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttgtg gtgcaacgcc aggacgtctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgactgggcc ccatattttg 420
atgtataaga aattgacgta a 441
<210>60
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>14_2B6
<400>60
atgattgaag tcaaaccaat aaatgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gggtacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cttcctttaa tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac actcgaagga 240
taccgtgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggacgtctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaagg cggggtctac gacataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>61
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>14_2G11
<400>61
atgattgaag tcaaaccaat aaatgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcag gggtgcgttt 120
cacctcggtg gatattaccg gggcaagctg gtcagcatcg cctcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac actcgaaggg 240
taccgtgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttgtg gtgcaacgcc aggacatctg cgagtgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgactgggcc ccatattttg 420
atgtataaga aattgacgta a 441
<210>62
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>14_3B2
<400>62
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
aggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcag gggtgcgttt 120
cacctcggtg gatattaccg gggcaagctg gtcagcatcg cctcctttca tcaggccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagga 240
taccgtgagc aaaaagcggg aagcacgctt atccgccatg ccgaagcgct tcttcggaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaagg cggggtctac gacataccgc cggccggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>63
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>14_4H8
<400>63
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gagcacgttt 120
cacctcggcg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac actcgaaggg 240
taccgtgagc aaaaagcggg aagcacgctc atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttgtg gtgcaacgcc aggacgtctg cgagcggcta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacacaccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>64
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>14_6A8
<400>64
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcgg gggtacgttt 120
cacctcggtg gatattaccg gggcaagcta gtcagcatcg cttcctttaa tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagga 240
taccgtgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttgtg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacacaccgc cggtcggacc tcatgttttg 420
atgtataaga aattgacgta a 441
<210>65
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>14_6B10
<400>65
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gggtacgttt 120
caccttggtg gatattaccg gggcaagctg atcagcatcg cttcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac actcgaagga 240
taccgtgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaagg cggggtctac gacatgccgc cggtcggacc tcatattttg 420
atgtataaga agttgacgta a 441
<210>66
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>14_6D4
<400>66
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cgaccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg aggcacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cttcctttaa tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagga 240
taccgtgagc aaaaagcggg cagtacgctt atccgccatg ccgaagcgct tcttcggaaa 300
aagggggcag acctcttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacacaccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>67
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>14_7A11
<400>67
atgattgaag tcaaaccaat aaacgcggag gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcag gggtgcgttt 120
cacctcggtg gatattaccg gggcaagctg gtcagcatcg cctcctttca tcaagccgaa 180
catccagagc ttgaaggcct aaaacagtat cagctgagag ggatggcgac actcgaaggg 240
taccgtgagc aaaaagcggg aagtacgctc atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag acctcttatg gtgcaacgcc aggacgtctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacacaccgc cgaccggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>68
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>14_7A1
<400>68
atgattgaag tcaaaccaat aaacgcggag gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcag gggtgcgttt 120
cacctcggtg gatattaccg gggcaagctg gtcagcatcg cctcctttca tcaagccgaa 180
catccagagc ttgaaggcct aaaacagtat cagctgagag ggatggcgac actcgaaggg 240
taccgtgagc aaaaagcggg aagtacgctc atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag acctcttatg gtgcaacgcc aggacgtctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacacaccgc cgaccggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>69
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>14_7A9
<400>69
atgattgaag tcaaaccaat aaacgcggag gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gggtacgttt 120
cacctcggcg gatattaccg gggcaagttg gtcagcatcg cctcctttca tcaagccaaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac actcgaaggg 240
taccgtgagc aaaaagcggg tagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggacgtctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacacaccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>70
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>14_7G1
<400>70
atgattgaag tcaaaccaat aaacgcagaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcag gggtgcgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cttcctttaa tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagttgagag ggatggcgac acttgaagag 240
taccgtgagc aaaaagcggg aagtacgctt atccgccatg ccgaagcgct tcttcggaaa 300
aagggggcag acctcttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacacaccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>71
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>14_7H9
<400>71
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gggtacgttt 120
cacctcggcg gatattaccg gggcaagctg gtcagcatcg cttcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagga 240
taccgtgagc aaaaagcggg aagcacgctc atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttgtg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>72
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>14_8F7
<400>72
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgcaag tatgaaaccg atttgctcgg gggtacgttt 120
cacctcggcg gatattaccg gggcaagctg gtcagcatcg cctcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagag 240
taccgcgagc aaaaagcggg cagtacgctt atccgccatg ctgaagcgct tcttcggaaa 300
aaaggcgcgg accttttgtg gtgcaacgcc aggacatctg caagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgactgggcc ccatattttg 420
atgtataaga aattgacgta a 441
<210>73
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>15_10C2
<400>73
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcag gggtgcgttt 120
cacctcggtg gatattaccg gggcaagctg gtcagcatcg cctcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagga 240
taccgtgagc aaaaagcggg aagtacgctc atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag acctcttatg gtgcaacgcc aggacaactg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg tgaagtcttc gacataccgc cgaccggacc ccatattttg 420
atgtataaga aattgacgta a 441
<210>74
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>15_10D6
<400>74
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcgg gggcacgttt 120
cacctaggtg gatattaccg gggcaagctg gtcagcatcg cctcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagag 240
taccgcgagc aaaaagcggg aagcacgctc atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag acctcttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>75
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>15_11F9
<400>75
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcag gggtgcgttt 120
caccttggtg gatattaccg gggcaagctg gtcagcatcg cctcctttaa tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac actcgaagga 240
taccgtgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaga 300
aagggggcag accttttatg gtgcaacgcc aggacatctg cgagcggcta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgaccggacc ccatattttg 420
atgtataaga aattgacgta a 441
<210>76
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>15_11H3
<400>76
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcatactc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcag gggtgcgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
cacccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaaggg 240
taccgtgagc aaaaagcggg cagtacgctt atccgccatg ccgaagcgct tcttcggaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc caactgggcc ccatattttg 420
atgtataaga aattgacgta a 441
<210>77
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>15_12A8
<400>77
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gggtacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagga 240
taccgtgagc aaaaagcggg cagtacgctt atccgccatg ccgaagcgct tcttcggaaa 300
aagggggcag acctcttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgaccggacc ccatattttg 420
atgtataaga aattgacgta a 441
<210>78
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>15_12D6
<400>78
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgtatg tatgaaaccg atttgctcag gggtgcgttt 120
cacctcggcg gatattaccg gggcaagctg gtcagcatcg cctcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaaggg 240
taccgtgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacacaccgc cggtcggacc tcatattttg 420
atgtataaga agttgacgta a 441
<210>79
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>15_12D8
<400>79
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcatactc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gggtacgttt 120
cacctcggcg gatattaccg gggcaagctg gtcagcatcg cctcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat caactgagag ggatggcgac acttgaagga 240
taccgtgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggacgtctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg caaagtctac gacataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>80
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>15_12D9
<400>80
atgattgaag tcaaaccaat aaacgcggag gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcag gggtacgttt 120
cacctcggcg gatattaccg gggcaagctg gtcagcatcg cctcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac actcgaagag 240
taccgcgagc aaaaagcggg aagcacgctc atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag acctcttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacata a 441
<210>81
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>15_3F10
<400>81
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcag gggtgcgttt 120
caccttggtg gatattaccg gggcaagctg atcagcatcg tttcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaaggg 240
taccgtgagc aaaaagcggg cagcacgctt atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacacaccgc cggccggacc tcatattttg 420
atgtatacga aattgacgta a 441
<210>82
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>15_3G11
<400>82
atgattgaag ttaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcatactc 60
cggccgaatc agccgcttga agcatgcaag tatgaaaccg atttgctcgg gggtacgttt 120
cacctcggcg gatattaccg gggcaagctg gtcagcatcg cctcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagag 240
taccgcgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttgtg gtgcaacgcc aggacgtctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>83
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>15_4F11
<400>83
atgattgaag tcaaaccaat aaacgcggaa gatacgtata agatcaggca ccgcatactc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggtg gatattaccg gggcaagctg gtcagcatcg cttcctttaa tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaaggg 240
taccgtgagc aaaaagcggg cagtacgctt atccgccatg ccgaagcgct tcttcggaag 300
aaaggcgcgg accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgaccggacc ccatattttg 420
atgtataaga aattgacgta a 441
<210>84
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>15_4H3
<400>84
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgcaag tatgaaaccg atttgctcgg gggtacgttt 120
cacctcggcg gatattaccg gggcaagctg gtcagcatcg cttcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagag 240
taccgcgagc aaaaagcggg aagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgactgggcc ccatattttg 420
atgtataaga aattgacgta a 441
<210>85
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>15_6D3
<400>85
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcatactc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gggtacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
cacccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagag 240
taccgcgagc aaaaagcggg aagcacgctc atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag acctcttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgaccggacc ccatattttg 420
atgtataaga aattgacgta a 441
<210>86
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>15_6G11
<400>86
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcag gggtgcgttt 120
cacctcggtg gatattaccg gggcaagctg gtcagcatcg cctcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagag 240
taccgcgagc aaaaagcggg aagcacgctc atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg caaagtctac gacataccgc cggtcggacc tcatattttg 420
atgtataaga agttgacgta a 441
<210>87
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>15_9F6
<400>87
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gggtacgttt 120
cacctcggcg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac actcgaagag 240
taccgcgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaga 300
aaaggcgcgg accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc ctgtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>88
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>15F5
<400>88
atgatcgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gggtacgttt 120
cacctcggtg ggtactaccg gggcaagctg atcagcatcg cttcctttca taaagccgaa 180
cattcagagc ttgagggcga agaacagtat cagctgagag ggatggcgac gcttgaagga 240
taccgtgagc aaaaagcggg cagtacgctt atccgctatg ccgaagagct tcttcgaaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggacatctg tgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgatcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>89
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>16A1
<400>89
atgattgaag tcaaacctat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcgg gggtacgctt 120
cacctcggtg gatattacca gggcaagctg atcagcatcg cttcctttca taaagccgaa 180
cattcagggc ttgagggcga agaacagtat cagctgagag ggatggcgac gctcgaaggg 240
taccgcgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcgaaaa 300
aaaggcgcgg accttttatg gtgcaatgcc aggacatctg tgagcggcta ctatgaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgatcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>90
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>16H3
<400>90
atgattgacg tcaaacctat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggcg gatattacca gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaaggg 240
taccgcgagc aaaaagcggg aagtacgctc atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaatgcc aggacatctg tgagcgggta ctatgaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgatcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>91
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>17C12
<400>91
atgattgaag tcaaaccaat aagcgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgtatg tatgaaaccg atttgctcgg gggtgcgttt 120
cacctcggtg gatattacca gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaaggg 240
taccgcgagc aaaaagcggg aagtacgctt atccgccatg ccgaagagct tcttcgaaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggacatctg tgagcgggta ctatgaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgatcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>92
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>18D6
<400>92
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cttcctttca taaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcaac gcttgaagga 240
taccgtgagc aaaaagcggg aagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacatctg cgagcggcta ctatgaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgatcggacc tcatattttg 420
atgtataaga aattggcata a 441
<210>93
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>19C6
<400>93
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gggtacgttt 120
cacctcggtg gatattaccg gggcaagctg atctgcatcg cctcctttca tcaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac gcttgaaggg 240
taccgcgagc aaaaagcggg aagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaatgcc aggacatctg tgagaggcta ctatgaaaag 360
ctcggcttca gcgaacaagg cggggtctac gatataccgc cgatcggacc tcatattttg 420
atgtataaga aattggcgta a 441
<210>94
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>19D5
<400>94
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ctgcattctc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcgg gggtacgttt 120
cacctcggtg gatattacca gggcaagctg atcagcatcg cttcctttca taaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac gcttgaaggg 240
taccgcgagc aaaaagcggg cagtacgctc atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaatgcc aggacatctg tgagcggcta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgatcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>95
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>20A12
<400>95
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcgg gggtacgttt 120
cacctcggtg gatattacca gggcaagctg atcagcatcg cttcctttca taatgccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac gcttgaaggg 240
taccgtgagc aaaaagcggg aagcacgctc atccgccatg ccgaagagct tcttcggaaa 300
aagggggtag accttttatg gtgcaacgcc aggacatctg tgagcgggta ctataaaaag 360
ctcggcttca gcgaacaagg cgggatctac gacataccgc cgatcggacc tcatattttg 420
atgtataaga aattggcata a 441
<210>96
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>20F2
<400>96
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcgg gggtacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cttcctttca tcaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaaggg 240
taccgcgagc aaaaagcggg aagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcag accttttatg gtgcaacgcc aggacatctg tgagcggcta ctatgaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgatcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>97
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>21E11
<400>97
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gggtgcgttt 120
cacctcggtg gatattacca gggcaagctg atcagcatcg cttcctttca tcaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac gcttgaagga 240
taccgtgagc aaaaagcggg aagcacgctc atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcag accttttatg gtgcaacgcc aggacatctg tgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgatcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>98
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>23H11
<400>98
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga ggcatgtatg tatgaaaccg atttgctcgg gggtacgttt 120
cacctcggtg gatattacca gggcaagctg atcagcatcg cttcctttca taaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac gcttgaaggg 240
taccgcgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tctccgaaaa 300
aaaggcgcgg accttttatg gtgcaatgcc aggacatctg cgagcggcta ctatgaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccac cgatcggacc tcatattttg 420
atgtataaga aattggcata a 441
<210>99
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>24C1
<400>99
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggcg gatattatcg ggacaggctg atcagcatcg cttcctttca tcaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac gcttgaaggg 240
taccgcgagc aaaaagcggg aagcacgctc atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacatctg tgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgatcggacc tcatattttg 420
atgtataaga aactgacgta a 441
<210>100
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>24C6
<400>100
atgattgaag tcaaacctat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcgg gggtacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cttcctttca tcaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac gcttgaaggg 240
taccgcgagc aaaaagcggg aagtacgctt atccgccatg ccgaagagct tcttcgaaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggatatctg tgagcggcta ctataaaaag 360
ctcggcttca gcgaacaagg cggggtctac gacataccgc cgatcggacc tcatattttg 420
atgtataaga aattggcata a 441
<210>101
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>24E7
<400>101
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga ggcatgcaag tatgaaaccg atttgctcgg gggcacgttt 120
catctcggtg gatattaccg gggcaagctg atcagcatcg cttcctttca taatgccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac gcttgaagga 240
taccgcgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaatgcc aggacatctg cgagcggcta ctatgaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgatcggacc tcatattttg 420
atgtataaga aattggcata a 441
<210>102
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>2_8C3
<400>102
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgtattctc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcgg gggtacgttt 120
cacctcggcg gatattatcg ggacaggctg atcagcatcg cctcctttca tcaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac gcttgaaggg 240
taccgcgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacatctg cgagcggcta ctatgaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgatcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>103
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>2H3
<400>103
atgattgaag tcaaaccgat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gggtacgttt 120
cacctcggtg gatattacca gggcaagctg atcagcaccg cttcctttca tcaagccgga 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaaggg 240
taccgcgagc gaaaagcggg aagtacgctc atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggatatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaagg cggggtctac gatataccgc cgatcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>104
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>30G8
<400>104
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgtatg tttgaaaccg atttgctcgg gggtgcgttt 120
cacctcggtg gatattacca gggcaagctg atcagcatcg cttcctttca tcaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac gcttgaaggg 240
taccgcgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcag accttttatg gtgcaacgcc aggacatctg tgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgatcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>105
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>3B_10C4
<400>105
atgattgaag tcagaccaat aaacgcggaa gatacgtatg agatcaggca ccgtattctc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagga 240
taccgtgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagcctac gacataccgc cgatcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>106
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>3B_10G7
<400>106
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcgg gggtacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac gcttgaaggg 240
taccgcgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaagg cggggtctac gacataccgc cgatcggacc ccatattttg 420
atgtataaga aattgacgta a 441
<210>107
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>3B_12B1
<400>107
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgtatg tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaaggg 240
taccgcgagc aaaaagcggg aagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgatcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>108
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>3B_12D10
<400>108
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgtattctc 60
cggccgaatc agccgctgga agcatgtatg tacgaaaccg atttgctcgg gggtgcgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cctcctttca tccagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagga 240
taccgtgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggatatctg cgagcgggta ctatgaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgatcggacc ccatattttg 420
atgtataaga aattgacgta a 441
<210>109
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>3B_2E5
<400>109
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgtatg tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagga 240
taccgtgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggcgcgg accttttatg gtgcaacgcc aggacatctg cgagcggcta ctatgaaaag 360
ctcggcttca gcaaacaggg cgaagtctac gacataccgc cgatcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>110
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>3C_10H3
<400>110
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgtattctc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagga 240
taccgtgagc aaaaagcggg aagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggatatctg cgagcggcta ctataaaaag 360
ctcggcttca gcgaacaagg cggggtctac gacataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>111
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>3C_12H10
<400>111
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaaggg 240
taccgtgggc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggacatctg cgagcggcta ctatgaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgatcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>112
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>3C_9H8
<400>112
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgtattctc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggcg gatattatca ggacaggctg atcagcatcg cctcctttca tcaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaaggg 240
taccgcgagc aaaaagcggg aagtacgctt atccgctatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggatatctg cgagcggcta ctatgaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgatcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>113
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>4A_1B11
<400>113
atgattgaag tcaaacctat aaacgcggaa gatacgtatg agatcaggca ccgcatactc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcgg gggtacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac gcttgaaggg 240
taccgcgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacatctg cgagcggcta ctatgaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgatcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>114
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>4A_1C2
<400>114
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggcg gatattatcg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac gcttgaagag 240
taccgcgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcag accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgatcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>115
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>4B_13E1
<400>115
atgattgaag tcaaacctat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cttcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagag 240
taccgcgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttgtg gtgcaacgcc aggatatctg cgagcggcta ctatgaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgatcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>116
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>4B_13G10
<400>116
ttacgtcaat ttcttataca tcaaaatatg aggtccgatc ggcggtatgt cgtagacttc 60
gccctgttcg ctgaagccga gctttttata gtacccgctc gcagatgtcc tggcgttgca 120
ccataaaagg tccgcgcctt ttttccgaag aagctcttcg gcatggcgga tgagcgtgct 180
tcccgctttt tgctcgcggt acccttcaag cgtcgccatc cctctcagct gatactgttt 240
ttggccttca agctctgaat gttcggcttg atgaaaggag gcgatgctga tcagcttgcc 300
ccggtaatat ccaccgaggt gaaacgtgcc cccgagcaaa tcagtttcat acttgcatgc 360
ttccagcggc tgattcggcc ggagaatgcg gtgcctgatc tcatacgtat cttccgcgtt 420
tattggtttg gcttcaatca t 441
<210>117
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>4B_16E1
<400>117
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gggtacgttt 120
cacctcggcg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaaggg 240
taccgcgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaagg cggggtctac gatataccgc cgatcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>118
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>4B_17A1
<400>118
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggcg gatattaccg gggcaagctg atcagcatcg cttcctttca tcaagccgag 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac gcttgaagag 240
taccgcgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacatctg cgagcggcta ctatgaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgatcggacc tcatattttg 420
atgtataaga aattgacata a 441
<210>119
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>4B_18F11
<400>119
atgattgaag tcaatccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcgg gggcacgtct 120
cacctcggcg gatattaccg gggcaagctg atcagcatcg cttcctttca taatgccgaa 180
cattcagagc ttgatggcca aaaacagtat cagctgagag ggatggcgac acttgaaggg 240
taccgcgagc aaaaagcggg aagcacgctc atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcag accttttatg gtgcaacgcc aggacatctg tgagcggcta ctatgaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgatcggacc tcatatttcg 420
atgtataaga aattgacgta a 441
<210>120
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>4B_19C8
<400>120
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggcg gatattaccg gggcaagctg atcagcatcg cttcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac gcttgaaggg 240
taccgcgagc aaaaagcggg aagcacgctc atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaagg cggggtctac gatataccgc cgatcggacc tcatattttg 420
atgtataaga aattggcata a 441
<210>121
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>4B_1G4
<400>121
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gggtgcgttt 120
cacctcggcg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaatccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac gcttgaaggg 240
taccgcgagc taaaagcggg aagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggatatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgatcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>122
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>4B_21C6
<400>122
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cttcctttca tcaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagag 240
taccgcgagc aaaaagcggg aagcacgctc atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggatatctg cgagcggcta ctataaaaag 360
ctcggcttca gcgaacaagg cggggtctac gatataccgc cgatcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>123
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>4B_2H7
<400>123
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
cattcagagc ttgaaggcca aaaacagtac cagctgagag ggatggcgac gcttgaaggg 240
taccgcgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacatctg cgagcggcta ctataaaaag 360
ctcggcttca gcgaacaagg cggggtctac ggcataccgc cgatcggacc tcatattttg 420
atgtataaga aattgacata a 441
<210>124
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>4B_2H8
<400>124
atgattgaag ccaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaactg atttgctcgg gggcacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac gcttgaaggg 240
taccgcgagc aaaaagcggg aagcacgctc atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgatcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>125
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>4B_6D8
<400>125
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcatactc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cttcctttca tcaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac gcttgaaggg 240
taccgcgagc aaaaagcggg tagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacatgg cgaagtctac gacataccgc cgatcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>126
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>4B_7E8
<400>126
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcatg tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cttcctttca tcaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaaggg 240
taccgcgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacatctg tgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgatcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>127
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>4C_8C9
<400>127
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcag gggtgcgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cttcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagga 240
taccgtgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacatctg cgagcggcta ctatgaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgatcggacc tcatattttg 420
atgtataaga aattaacata a 441
<210>128
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>4H1
<400>128
atgattgagg tgaaaccgat taacgcagag gagacctatg aactaaggca taggatactc 60
agaccacacc agccgataga ggtttgtatg tatgaaaccg atttacttcg tggtgcgttt 120
cacttaggcg gcttttacag gggcaagctg atttccatag cttcattcca ccaggccgag 180
catccagaac tccagggcca gaaacaatac caactccgag gtatggctac cttggaaggt 240
tatcgtgacc agaaagcggg atcgagccta attaaacacg ctgaacagat ccttcggaag 300
cggggggcgg acatgctatg gtgcaatgcg cggacatccg ccgctggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agaggtattt gaaacgccgc cagtaggacc tcacatcgta 420
atgtataaac gcctcacata a 441
<210>129
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>6_14D10
<400>129
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgtatg tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggtg gatattaccg aggcaagctg atcagcatcg cctccttcca tcaagccgaa 180
cattcagagc ttgaaggcca taaacagtat cagctgagag ggatggcgac acttgaagag 240
taccgcgagc aaaaagcggg aagcacgctc atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacatctg cgagcggcta ctataaaaag 360
ctcggcttca gcgaacaagg cggggtctac gacataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>130
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>6_15G7
<400>130
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgtaag tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggcg gatattaccg gggcaagctg atcagcatcg cttcctttca tcaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaaggg 240
taccgcgagc aaaaagcggg aagcacgctc atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>131
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>6_16A5
<400>131
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gggtacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cctcctttca ccaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagga 240
taccgtgagc aaaaagcggg aagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaagg cggggtctac gacataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>132
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>6_16F5
<400>132
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cttcctttca tcaagccgta 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagga 240
taccgtgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacatctg cgagcggcta ctataaaaag 360
ctcggcttca gcgaacaagg cggggtctac gacataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>133
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>6_17C5
<400>133
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgcaag tatgaagccg atttgctcgg gggcacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cttcctttca tcaagccgag 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagga 240
aaccgtgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacgtaccgc cgatcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>134
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>6_18C7
<400>134
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcagg tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggtg gatattatcg gggcaagctg atcagcatcg cttcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac gcttgaagga 240
taccgtgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggatatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtttac gacataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>135
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>6_18D7
<400>135
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcgg gggtacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaaggg 240
taccgcgagc aaaaagcggg aagcacgctc atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaagg cggggtctac gacataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>136
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>6_19A10
<400>136
atgattgaag ccaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcgg gggtacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaaggg 240
taccgcgagc aaaaagcggg aagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgaccggacc ccatattttg 420
atgtataaga aattgacgta a 441
<210>137
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>6_19B6
<400>137
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcag gggtgcgttt 120
cacctcggtg gatattatcg gggcaagctg atcagcatcg cttcctttca tcaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac actcgaagga 240
taccgtgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggcgcag accttttatg gtgcaacgcc aggacatctg cgagcggcta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>138
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>6_19C3
<400>138
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gggtacgttt 120
cacctcggcg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagga 240
taccgtgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgatcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>139
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>6_19C8
<400>139
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gggtacgtta 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagga 240
taccgtgagc aaaaagcggg cagtacgctt atccgccaag ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaagg cggggtctac gacataccgc cggtcggacc tcatattttg 420
atgtataagg aattgacgta a 441
<210>140
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>6_20A7
<400>140
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcag gggcacgttt 120
cacctcggcg gatattaccg gggcaagctg atcagcatcg cttcctttca tcaagccgaa 180
cattcagatc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagag 240
taccgcgagc aaaaagcggg aagcacgctc atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>141
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>6_20A9
<400>141
atgattgaag tcaaaccaat aaacgcggga gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaaggg 240
taccgcgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tctacggaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaagg cggggtctac gacataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>142
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>6_20H5
<400>142
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggcg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagga 240
taccgtgagc aaaaagcggg aagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggacatctg cgagcggcta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgatcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>143
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>6_21F4
<400>143
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcgttctc 60
cggccgaatc agccgctgga agcatgtatg tatgaaaccg atttgctcgg gggtgcgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaaggg 240
taccgcgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacgtaccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>144
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>6_22C9
<400>144
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc ggccgcttga agcatgtatg tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
catccagggc ttgaaggcaa aaaacagtat cagctgagag ggatggcgac acttgaagag 240
taccgcgagc aaaaagcggg aagcacgctt atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggacttccg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaagg aggggtctac gacataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>145
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>6_22D9
<400>145
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgtattctc 60
cggccgaatc agccgctgga agcatgcatg tatgaaaccg atttgctcga gggcacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgag 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagga 240
taccgtgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>146
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>6_22H9
<400>146
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac gcttgatgag 240
taccgcgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcag accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgatcggacc ccatattttg 420
atgtataaga aattgacgta a 441
<210>147
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>6_23H3
<400>147
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgtatg tatggaactg atttgctcgg gggcacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cttcctttca tcaagccgag 180
caaccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaaggg 240
taccgcgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgagcaagg cggggtctac gacataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>148
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>6_23H7
<400>148
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cttcctttca tcaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac gcttgaagga 240
taccgcgagc aaaaagcggg aagtacgctt atccgccatg cagaagagat tcttcggaaa 300
aaaggcgcgg acctcttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaagg cggggtctac gacataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>149
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>6_2H1
<400>149
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcgttctc 60
cggccgaatc agccgctgga agcatgtatg tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaaccgtat cagctgagag ggatggcgac acttgaagga 240
taccgcgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaaatctac gacataccgc cgatcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>150
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>6_3D6
<400>150
atgattgaaa tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcgg gggtacgttt 120
cacctcggtg gatattaccg aggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac tcttgaagga 240
taccgtgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaggtctac gacataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>151
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>6_3G3
<400>151
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgtatg tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagga 240
taccgtgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggacatctg cgagcggcta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>152
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>6_3H2
<400>152
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgtatg tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagag 240
taccgcgagc aaaaagcggg aagcacgctc atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag acctcttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacata a 441
<210>153
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>6_4A10
<400>153
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggtg gatattaccg gggcaaactg atcagcatcg cctcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac gcttgaagga 240
taccgtgagc aaaaagcggg aagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggacatctg cgagcggcta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>154
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>6_4B1
<400>154
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcgtactc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggtg gatattaccg gggcaagctg atcggcatcg cttcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaaggg 240
taccgcgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacatctg cgagcggcta ctatgaaaag 360
ctcggcttca gcggacaggg cgaagtctac gacataccgc cgatcggacc tcatattttg 420
atgtataaga aattgacata a 441
<210>155
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>6_5D11
<400>155
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cttcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagag 240
taccgcgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgatcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>156
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>6_5F11
<400>156
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgtatg tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggtg gatattaccg gggcaagcta atcagcatcg cttcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagag 240
taccgcgagc aaaaagcggg aagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtccac gacataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>157
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>6_5G9
<400>157
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgtatg tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggcg gatattaccg gggcaagcta atcagcatcg cctcctttca tcaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac gcttgaagag 240
taccgtgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggatatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaagg cggggtctac gacataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>158
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>6_6D5
<400>158
atgattgaag tcaaaccaat aaacgcggaa gatgcgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaactg atttgctcgg gggcacgttt 120
cacctcggcg gatattaccg gggcaagctg atcagcatcg cttcctttca tcaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaaggg 240
taccgcgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttgtg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaagg cggggtctac gacataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>159
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>6_7D1
<400>159
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcag gggtgcgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cttcctttca tcaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagga 240
taccgtgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaagg cggggtctac gacataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>160
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>6_8H3
<400>160
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgtatg tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac gcttgaaggg 240
taccgcgagc aaaaagcggg aagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaagg cggggtctac gacataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>161
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>6_9G11
<400>161
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gggcacgctt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cttcctttca tcaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac gcttgaaggg 240
taccgcgagc aaaaagcggg aagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaagg cgaagtctac gacataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>162
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>6F1
<400>162
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcgg gggtacgttt 120
cacctcggtg gatattaccg gggcaagctg gtctgcatcg cttcctttca taaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac gcttgatgga 240
taccgcgagc aaaaagcggg aagcacgctc atccgccatg ccgaagagct tcttcgaaaa 300
aaaggcgcgg accttttatg gtgcaatgcc aggacatctg tgagcggcta ctatgaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>163
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>7_1C4
<400>163
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgtatg tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cttcctttca tcaagccgag 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagag 240
taccgcgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaagg cggggtctac gatataccgc cgatcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>164
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>7_2A10
<400>164
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaactg atttgctcgg gggcacgttt 120
catctcggtg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac gcttgaaggg 240
taccgcgagc aaaaagcggg aagcacgctc atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaagg cggggtctac gatataccgc cgatcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>165
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>7_2A11
<400>165
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cttcctttca tcaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaaggg 240
taccgcgagc aaaaagcggg aagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaagg cggggtctac gacataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>166
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>7_2D7
<400>166
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac gcttgaaggg 240
taccgtgagc aaaaagcggg aagtacgctc atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggacatctg cgagtgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>167
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>7_5C7
<400>167
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagga 240
taccgtgagc aaaaagtggg aagcacgctc atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaagg cggggtctac gatataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>168
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>7_9C9
<400>168
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg aaatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cttcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaaggg 240
taccgcgagc aaaaagcggg aagtacgctc atccgccatg ccgaagagct tctacggaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgatcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>169
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>9_13F10
<400>169
atgattgaag tcaaaccaat aaacgcggag gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcag gggtgcgttt 120
caccttggtg gatattaccg gggcaagctg gtcagcatcg cctcctttca tcaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagag 240
taccgcgagc aaaaagcggg aagcacgctc atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag acctcttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgactgggcc ccatattttg 420
atgtataaga aattgacgta a 441
<210>170
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>9_13F1
<400>170
atgattgaag tcaaaccaat aaacgcggag gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcag gggtgcgttt 120
caccttggtg gatattaccg gggcaagctg gtcagcatcg cctcctttca tcaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagag 240
taccgcgagc aaaaagcggg aagcacgctc atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag acctcttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgactgggcc ccatattttg 420
atgtataaga aattgacgta a 441
<210>171
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>9_15D5
<400>171
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga cgcatgcaag tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaaggg 240
taccgcgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag acctcttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>172
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>9_15D8
<400>172
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcatactc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcgg gggtacgttt 120
cacctcggcg gatattaccg gggcaagctg gtcagcatcg cctcctttca tcaagctgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaaggg 240
taccgtgagc aaaaagcggg cagtacgctt atccgccatg ccgaagcgct tcttcggaag 300
aaaggcgcgg accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacacaccgc cggtcggacc ccatattttg 420
atgtataaga agttgacgta a 441
<210>173
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>9_15H3
<400>173
atgattgaag tcaagccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atatgctcag gggtgcgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagag 240
taccacgagc aaaaagcggg aagcacgctc atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttta gcgaacaggg cgaagtctac aacacaccgc cggttggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>174
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>9_18H2
<400>174
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgtatg tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggcg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
catccagagc ttgtaggcca aaaacagtat cagctgagag ggatggcgac acttgaagga 240
taccgtgagc aaaaagcggg cagtacactt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>175
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>9_20F12
<400>175
atgattgaag taaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcgttctc 60
cggccgaatc agccgctgga agcatgtatg tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggtg gatattaccg gggcgagctg gtcagcatcg cttcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaaggg 240
taccgtgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttgtg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaagg cggggtctac gacataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>176
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>9_21C8
<400>176
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgtatg tatgaaactg atttgctcgg gggcacgttt 120
cacctcggcg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac actcgaagga 240
taccgcgagc aaaaagcggg cagtacgcta atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag acctcttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgatcaggg cgaagtctac gacataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>177
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>9_22B1
<400>177
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agataaggca ccgcatcctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggtg gatattaccg gggcaagctg gtcagcatcg cctcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaaggg 240
taccgtgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacttaccgc cgaccggacc ccatattttg 420
atgtataaga aattgacgta a 441
<210>178
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>9_23A10
<400>178
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gggcacgctt 120
cacctcggtg gatattaccg gggcaagctg gtcagcattg cttcctttca tcaagccgaa 180
catccagagc ttgagggcca aaaacagtat cagctgagag ggatggcgac acttgaaggg 240
taccgcgggc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaatgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaagg cggggtctac gacataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>179
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>9_24F6
<400>179
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
aggccgaatc agccgctaga agcatgcaag tatgaaaccg atttgctcag gggtgcgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagga 240
taccgtgagc aaaaagcggg cagtacgctt atccgccatg ccgaagcgct tcttcggaaa 300
aaaggcgcgg accttttgtg gtgcaacgcc aggacgtctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgaccggacc ccatattttg 420
atgtataaga aattgacgta a 441
<210>180
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>9_4H10
<400>180
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaactg atttgctagg gggtacgctt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaaggg 240
taccgtgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggcgcgg accttatatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacata a 441
<210>181
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>9_4H8
<400>181
atgattgaag tcaaaccaat aaatgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcgg aggcacgttt 120
cacctaggtg gatattaccg gggcaagctg atcagcatcg cttcctttaa tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaaggg 240
taccgtgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacata a 441
<210>182
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>9_8H1
<400>182
atgattgaag tcaaaccaat aaccgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gggtacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac actagaaggg 240
taccgcgagc aaaaagcggg cagtacgctc atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc agaacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgaccggacc ccatattttg 420
atgtataaga aattgacgta a 441
<210>183
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>9_9H7
<400>183
atgattgaag tcaaaccaat aaacgcggaa gatgcgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gagcacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagag 240
taccgcgagc aaaaagcggg aagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc ctgtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>184
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>9C6
<400>184
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gggtacgttt 120
cacctcggtg gatattaccg gggcaagctg atctgcatcg cctcctttca tcaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac gcttgaaggg 240
taccgcgagc aaaaagcggg aagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaatgcc aggacatctg tgagaggcta ctatgaaaag 360
ctcggcttca gcgaacaagg cggggtctac gatataccgc cgatcggacc tcatattttg 420
atgtataaga aattggcgta a 441
<210>185
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>9H11
<400>185
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgttgga agcatgcaag tatgaaaccg atttgctcgg gggtacgttt 120
cacctcggcg gatattaccg gggcaagctg atcagcatcg cttcctttca taaagccgaa 180
cattcagagc ttgagggcga agaacagtat cagctgagag ggatggcgac gcttgaagga 240
taccgtgagc aaaaagcggg aagcacgctc atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaatgcc aggacatctg tgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgatcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>186
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>0_4B10
<400>186
atgatagaag tgaaaccgat taacgcagag gatacctatg aactaaggca taaaatactc 60
agaccaaacc agccgataga agcgtgtatg tatgaaagcg atttacttcg tggtgcattt 120
cacttaggcg gcttttacag gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagacc tcgaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgatc agaaagcggg atcgactcta attaaacacg ctgaagaaat tcttcgtaag 300
aggggggcgg acatgctttg gtgcaatgcg cggacaaccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agagatattt gatacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggctcacata a 441
<210>187
<211>438
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>0_5B11
<400>187
atgatagagg tgaaaccgat taacgcagag gatacctatg aactaaggca taaaatactc 60
agaccaaacc agccgataga agcgtgtatg tatgaaagcg atttacttcg tggtgcattt 120
cacttaggcg gcttttacgg gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagacc tcgaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgatc agaaagcggg atcgactcta attaaacacg ctgaacaact tcttcgtaag 300
aggggggcgg acatgctttg gtgcaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agaggtattt gaaacgccgc cagtaggacc tcacatcctg 420
atgtataaaa agatcaca 438
<210>188
<211>438
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>0_5B3
<400>188
atgctagagg tgaaaccgat taacgcagag gatacctatg aactaaggca tagaatactc 60
agaccaaacc agccgataga agcgtgtatg tatgaaaccg atttacttcg tggtgcattt 120
cacttaggcg gcttttacag gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagaac tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgatc agaaagcggg atcgagtcta attaaacacg ctgaacaact tcttcgtaag 300
aggggggcgg acttgctttg gtgcaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agaggtattt gatacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggatcaca 438
<210>189
<211>438
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>0_5B4
<400>189
atgctagagg tgaaactgat taacgcagag gatacctatg aactaaggca tagaatactc 60
agaccaaacc agccgttaga agcgtgtatg tatgaaaccg atttacttcg tggtgcattt 120
cacttaggcg gcttttacag gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagacc tcgaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tttcgtgatc agaaagcggg atcgagtcta attaaacacg ctgaagaaat tcttcgtaag 300
aggggggcga acttgctttg gtgtaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agaggtattt gatacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggatcaca 438
<210>190
<211>438
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>0_5B8
<400>190
atgatagagg tgaaaccgat taacgcagag gatacctatg aactaaggca taaaatactc 60
agaccaaacc agccgataga agcgtgtatg tatgaaagcg atttacttcg tggtgcattt 120
cacttaggcg gcttttacag gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagacc tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgatc agaaagcggg atcgagtcta attagacacg ctgaacaaat tcttcgtaag 300
aggggggcgg acttgctttg gtgcaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agagatattt gatacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggctcaca 438
<210>191
<211>438
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>0_5C4
<400>191
atgatagagg tgaaaccgat taacgcagag gatacctatg aactaaggca taaaatactc 60
agaccaaacc agccgttaga agcgtgtatg tatgaaaccg atttacttcg tggtgcattt 120
cacttaggcg gcttttacag gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcaggcc tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgagtata attaaacacg ctgaagaaat tcttcgtaag 300
aagggggcgg acttgctttg gtgcaatgcg cggacgtccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agagatattt gacacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggatcaca 438
<210>192
<211>438
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>0_5D11
<400>192
atgatagagg tgaaaccgat taacgcagag gatacctatg aactaaggca tagaatactc 60
agaccaaacc agccgataga agcgtgtatg tatgaaagcg atttacttcg tggtgcattt 120
cacttaggcg gcttttacag gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagacc tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgactcta attagacacg ctgaacaact tcttcgtaag 300
aggggggcgg acttgctttg gtgcaatgcg cggacatccg cctcaggcta ctacaaaagg 360
ttaggcttca gcgagcaggg agaggtattt gatacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggctcaca 438
<210>193
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>0_5D3
<400>193
atgctagagg tgaaaccgat taacgcagag gatacctatg aactaaggca tagaatactc 60
agaccaaacc agccgataga agcgtgtatg tatgaaagcg atttacttcg tggtgcattt 120
cacttaggcg gctattacag gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagaac tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgagtcta attaaacacg ctgaagaaat tcttcgtaag 300
aggggggcgg acttgctttg gtgtaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agagatattt gaaacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggatcacata a 441
<210>194
<211>438
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>0_5D7
<400>194
atgatagaag tgaaaccgat taacgcagag gagacctatg aactaaggca tagaatactc 60
agaccaaacc agccgataga agcgtgtatg tatgaaaccg atttacttcg tggtgcattt 120
cacttaggcg gcttttacag gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagaac tcgaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgatc agaaagcggg atcgagtcta attagacacg ctgaacaact tcttcgtaag 300
aagggggcga atatgctttg gtgtaatgcg cggacaaccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agagatattt gatacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggatcaca 438
<210>195
<211>438
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>0_6B4
<400>195
atgctagagg tgaaaccgat taacgcagag gatacctatg aactaaggca tagaatactc 60
agaccaaacc agccgataga agcgtgtatg tatgaaagcg atttacttcg tggtgcactt 120
cacttaggcg gcttttacag gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagacc tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tttcgtgatc agaaagcggg atcgagtcta attagacacg ctgaacaaat tcttcgtaag 300
aggggggcgg acttgctttg gtgcaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg aaaggtattt gatacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggatcaca 438
<210>196
<211>438
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>0_6D10
<400>196
atgctagagg tgaaaccgat taacgcagag gatacctatg aactaaggca taaaatactc 60
agaccaaacc agccgttaga agtgtgtatg tatgaaaccg atttacttcg tggtgcattt 120
cacttaggcg gcttttacag gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagacc tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgatc agaaagcggg atcgagtcta attagacacg ctgaacaaat tcttcgtaag 300
aggggggcgg acatgctttg gtgcaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agaggtattt gaaacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggctcaca 438
<210>197
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>0_6D11
<400>197
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gggcacgctt 120
cacctcggtg gatattaccg gggcaagctg gtcagcatcg cttcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac gcttgaaggg 240
taccgtgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacatctg cgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cggtcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>198
<211>438
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>0_6F2
<400>198
atgatagagg tgaaaccgat taacgcagag gatacctatg aactaaggca tagaatactc 60
agaccaaacc agccgataga agcgtgtatg tatgaaagcg atttacttcg tggtgcattt 120
cacttaggcg gctattacag gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagaac tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tttcgtgagc agaaagcggg atcgactcta attagacacg ctgaacaaat tcttcgtaag 300
aggggggcgg acatgctttg gtgcaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agagatattt gatacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggatcaca 438
<210>199
<211>438
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>0_6H9
<400>199
atgatagagg tgaaaccgat taacgcagag gatacctatg aactaaggca taaaatactc 60
agaccaaacc agccgataga agcgtgtatg tatgaaaccg atttacttcg tggtgcattt 120
cacttaggcg gcttttacgg gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagacc tcgaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgactcta attagacacg ctgaagaaat tcttcgtaag 300
aagggggcga acttgctttg gtgcaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agaggtattt gacacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggctcaca 438
<210>200
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>10_4C10
<220>
<221>混杂的特征
<222>134,313
<223>n=A,T,C或G
<400>200
atgatagagg tgaaaccgat taacgcagag gatacctatg aactaaggca taaaatactc 60
agaccaaacc agccgttaga agtgtgtatg tatgaaaccg atttacttcg tggtgcattt 120
cacttaggcg gctnttacag gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagaac tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgatc agaaagcggg atcgagtcta attaaacacg ctgaacaaat tcttcgtaag 300
aggggggcgg acntgctttg gtgcaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agagatattt gatacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggctcacata a 441
<210>201
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>10_4D5
<400>201
atgatagagg tgaaaccgat taacgcagag gatacctatg aactaaggca tagaatactc 60
agaccaaacc agccgataga agtgtgtatg tatgaaaccg atttacttcg tggtgcattt 120
cacttaggcg gcttttacag gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagacc tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgactcta attagacacg ctgaacaaat tcttcgtaag 300
aggggggcgg acttgctttg gtgcaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agaggtattt gatacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggatcacata a 441
<210>202
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>10_4F2
<400>202
atgctagagg tgaaaccgat taacgcagag gatacctatg aactaaggca tagaatactc 60
agaccaaacc agccgataga agcgtgtatg tttgaaagcg atttacttcg tggtgcattt 120
cacttaggcg gcttttacag gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagaac tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgagtcta attagacacg ctgaagaaat tcttcgtaag 300
aggggggcgg acatgctttg gtgtaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agagatattt gaaacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggctcacata a 441
<210>203
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>10_4F9
<400>203
atgatagagg tgaaaccgat taacgcagag gatacctatg aactaaggca tagaatactc 60
agaccaaacc agccgataga agtgtgtatg tatgaaaccg atttacttcg tggtgcattt 120
cacttaggcg gcttttacag gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagaac tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tttcgtgagc agaaagcggg atcgagtcta attagacacg ctgaacaaat tcttcgtaag 300
aggggggcgg acttgctttg gtgtaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agagatattt gatacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggctcacata a 441
<210>204
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>10_465
<400>204
atgatagagg tgaaaccgat taacgcagag gatacctatg aactaaggca tagaatactc 60
agaccaaacc agccgataga agcgtgtatg tttgaaagcg atttacttcg tggtgcattt 120
cacttaggcg gctattacag gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagacc tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
taccgcgatc agaaagcggg atcgagtcta attagacacg ctgaacaaat tcttcgtaag 300
aggggggcgg acttgctttg gtgtaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agagatattt gatacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggctcacata a 441
<210>205
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>10_4H4
<400>205
atgctagagg tgaaaccgat taacgcagag gatacctatg aactaaggca taaaatactc 60
agaccaaacc agccgttaga agtgtgtatg tatgaaaccg atttacttcg tggtgcattt 120
cacttaggcg gcttttacag gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagaac tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgagtcta attaaacacg ctgaagaaat tcttcgtaag 300
aggggggcgg acttgctttg gtgcaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agaggtattt gatacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggatcacata a 441
<210>206
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>11_3A11
<400>206
atgatagaag tgaaaccgat taacgcagag gatacctatg aactgaggca taaaatactc 60
agaccaaacc agccgataga agtgtgtatg tatgaaagcg atttacttcg tggtgcattt 120
cacttaggcg gcttttacag gggcaaactg atttccatag cgtcattcca ccaggccgag 180
cacccagacc tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgatc agaaagcggg atcgagtcta attaaacacg ctgaacaaat tcttcgtaag 300
aggggggcgg acttgctttg gtgcaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agaggtattt gaaacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggctcacata a 441
<210>207
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>11_3B1
<400>207
atgctagagg tgaaaccgat taacgcagag gatacctatg aactgaggca tagaatactc 60
agaccaaacc agccgataga agcgtgtatg tttgaaaccg atttacttcg tggtgcattt 120
cacttaggcg gcttttacag gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagacc tccaaggcca gaaacagtac caactccgag gtatggctac cttggaaggt 240
tttcgtgagc agaaagcggg atcgactcta attagacacg ctgaagaaat tcttcgtaag 300
aggggggcgg acttgctttg gtgcaatgcg cggacatccg cctcaggcta ctacaaaagg 360
ttaggcttca gcgagcaggg agagatattt gacacgccgc cagtagggcc tcacatcctg 420
atgtataaaa ggctcacata a 441
<210>208
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>11_3B5
<400>208
atgatagagg tgaaaccgat taacgcagag gatacctatg aactaaggca tagaatactc 60
agaccaaacc agccgataga agcgtgtatg tttgaaagcg atttacttcg tggtgcattt 120
cacttaggcg gctattacag gggcaaactg atttccatag cgtcattcca ccaggccgag 180
cactcggaac tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgatc agaaagcggg atcgagtcta attagacacg ctgaacaaat tcttcgtaag 300
aggggggcgg acatgctttg gtgcaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agaggtattt gatacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggatcacata a 441
<210>209
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>11_3C12
<400>209
atgatagagg tgaaaccgat taacgcagag gatacctatg aactaaggca tagaatactc 60
agaccaaacc agccgttaga agtgtgtatg tatgaaaccg atttacttcg tggtgcattt 120
cacttgggcg gcttttacgg gggcaaactg atttccatag cgtcattcca ccaggccgag 180
cacccagacc tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgatc agaaagcggg atcgagtcta attagacacg ctgaacaact tcttcgtaag 300
aggggggcgg acttgctttg gtgcaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agagatattc gaaacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggatcacata a 441
<210>210
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>11_3C3
<400>210
atgatagaag tgaaaccgat taacgcagag gatacctatg aactaaggca taaaatactc 60
agaccaaacc agccgataga agcgtgtatg tatgaaagcg atttacttcg tggtgcactt 120
cacttaggcg gctattacag gggcaaactg atttccatag cgtcattcca ccaggccgag 180
cactcagaac tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgagtcta attaaacacg ctgaagaaat tcttcgtaag 300
aggggggcgg acttgctttg gtgcaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agaggtattt gacacgccgc cagtaggacct cacatcctg 420
atgtataaaa ggatcacata a 441
<210>211
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>11_3C6
<400>211
atgctagagg tgaaaccgat taacgcagag gatacctatg aactaaggca taaaatactc 60
agaccaaacc agccgataga agcgtgtatg tttgaaagcg atttacttcg tggtgcattt 120
cacttaggcg gcttttacgg gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagacc tcgaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgactcta attagacacg ctgaagaaat tcttcgtaag 300
aggggggcgg acttgctttg gtgcaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agagatattt gatacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggatcacata a 441
<210>212
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>11_3D6
<400>212
atgatagagg tgaaaccgat taacgcagag gatacctatg aactaaggca tagaatactc 60
agaccaaacc agccgataga agtgtgtatg tatgaaaccg atttacttcg tggtgcattt 120
cacttaggcg gcttttacag gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagacc tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgagtcta attaaacacg ctgaacaaat tcttcgtaag 300
aggggggcgg acttgctttg gtgcaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agaggtattt gatacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggctcacata a 441
<210>213
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>1_1G12
<400>213
atgctagagg tgaaaccgat taacgcagag gatacctatg aactaaggca tagaatactc 60
agaccaaacc agccgataga agtgtgtatg tatgaaaccg atttacttcg tggtgcattt 120
cacttaggcg gcttttacgg gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagaac tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgatc agaaagcggg atcgagtcta attaaacacg ctgaagaaat tcttcgtaag 300
aggggggcgg acttgctttg gtgtaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agaggtattt gaaacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggctcacata a 441
<210>214
<211>438
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>1_1H1
<400>214
atgatagaag tgaaacctat taacgcagag gagacttacg aacttcgaca caagatcctg 60
cgccctaatc agccgataga ggcatgcatg tatgaaagcg atctgctgcg gggctcgttc 120
catttgggcg ggttctatcg tggccaattg atctcgattg cgagtttcca caaagctgaa 180
cactcagaac tgcaagggca aaagcagtat caattacgag ggatggcgac cctcgaagga 240
ttccgtgagc agaaggctgg ctcttcgctt attaggcacg ccgaggagat actacggaat 300
aaaggggcag atctgctttg gtgtaatgca cgcacgacag cctccggtta ctataaaagg 360
cttggtttta gtgagcacgg cgaagttttc gaaaccccgc cggttgggcc gcacattctt 420
atgtacaaaa gaatcact 438
<210>215
<211>438
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>1_1H2
<400>215
atgatagaag tgaaacctat taacgcagag gatacttacg aacttcgaca caggatcctg 60
cgccctaatc agccgttaga ggcatgcatg tatgaaagcg atctgctgcg gggctcgttc 120
catttgggcg ggttctatcg tggcaaattg atctcgattg cgagtttcca ccaagctgaa 180
cactcagaac tggaagggca aaagcagtat caattacgag ggatggcgac cctcgaagga 240
ttccgtgagc agaaggctgg ctcttcgctt attaggcacg ccgaggagat actacggaaa 300
agaggggcag atctgctttg gtgtaatgca cgcacgacag ccgccggtta ctataaaaag 360
cttggtttta gtgagcaggg cgaaattttc gacaccccgc cggttgggcc gcacattctt 420
atgtacaaaa gaatcact 438
<210>216
<211>438
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>1_1H5
<400>216
atgatagaag tgaaacctat taacgcagag gatacttacg aaattcgaca caggatcctg 60
cgccctaatc agccgttaga ggcatgcatg tatgaaagcg atctgctgcg gggctcgttc 120
catttgggcg ggttctatcg tggcaaattg atctcgattg cgagtttcca ccaagctgaa 180
cactcagacc tggaagggca aaagcagtat caattacgag ggatggcgac cctcgaagga 240
taccgtgatc agaaggctgg ctcttcgctt attaggcacg ccgagcagat actacggaaa 300
agaggggcag atctgctttg gtgcaatgca cgcacgacag ccgccggtta ctataaaagg 360
cttggtttta gtgagcaggg cgaagttttc gacaccccgc cggttgggcc gcacattctt 420
atgtacaaaa aactcact 438
<210>217
<211>438
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>1_2A12
<400>217
atgatagaag tgaaacctat taacgcagag gatacttacg aacttcgaca caggatcctg 60
cgccctaatc agccgataga ggcatgcatg tatgaaagcg atctgctgcg gggctcgttc 120
catttgggcg ggttctatcg tggcaaattg atctcgattg cgagtttcca ccaagctgaa 180
cagtcagaac tggaagggca aaagcagtat caattacgag ggatggcgac cctcgaagga 240
taccgtgatc agaaggctgg ctctacgctt attaagcacg ccgaggagat actacggaaa 300
aaaggggcag atctgctttg gtgcaatgca cgcacgtcag ccgccggtta ctataaaagg 360
cttggtttta gtgagcaggg cgaaattttc gacaccccgc cggttgggcc gcacattctt 420
atgtacaaaa gactcact 438
<210>218
<211>438
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>1_2B6
<400>218
atgatagaag tgaaacctat taacgcagag gagacttacg aacttcgaca caagatcctg 60
cgccctaatc agccgttaga ggcatgcatg tatgaaaccg atctgctgcg gggctcgttc 120
catttgggcg ggttctatcg tggcaaattg atctcgattg cgagtttcca ccaagctgaa 180
cactcagaac tggaagggca aaagcagtat caattacgag ggatggcgac cctcgaagga 240
ttccgtgatc agaaggctgg ctcttcgctt attaagcacg ccgaggagat actacggaaa 300
agaggggcag atctgctttg gtgcaatgca cgcacgtcag cctccggtta ctataaaaag 360
cttggtttta gtgagcaggg cgaaattttc gaaaccccgc cggttgggcc gcacattctt 420
atgtacaaaa gactcact 438
<210>219
<211>438
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>1_2C4
<400>219
atgctagaag tgaaacctat taacgcagag gagacttacg aacttcgaca caagatcctg 60
cgccctaatc agccgataga ggcatgcatg tatgaaaccg atctgctgcg gggctcgttc 120
catttgggcg ggttctatcg tggccaattg atctcgattg cgagtttcca ccaagctgaa 180
cactcagacc tgcaagggca aaagcagtat caattacgag ggatggcgac cctcgaagga 240
taccgtgagc agaaggctgg ctctacgctt attaagcacg ccgaggagct actacggaaa 300
aaaggggcag atctgctttg gtgcaatgca cgcacgacag ccgccggtta ctataaaaag 360
cttggtttta gtgagcaggg cgaagttttc gacaccccgc cggttgggcc gcacattctt 420
atgtacaaaa aaatcact 438
<210>220
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>1_2D2
<400>220
atgatagaag tgaaacctat taacgcagag gatacttacg aacttcgaca caagatcctg 60
cgccctaatc agccgttaga ggcatgcatg tatgaaagcg atctgctgcg gagcgcattc 120
catttgggcg ggttctatcg tggcaaattg atctcgattg cgagtttcca caaagctgaa 180
cactcagaac tgcaagggca aaagcagtat caattacgag ggatggcgac cctcgaagga 240
taccgtgatc agaaggctgg ctcttcgctt attaggcacg ccgaggagat actacggaaa 300
agaggggcag atatgctttg gtgcaatgca cgcacgtcag ccgccggtta ctataaaagg 360
cttggtttta gtgagcaggg cgaagttttc gacaccccgc cggttgggcc gcacattctt 420
atgtacaaaa gaatcactta a 441
<210>221
<211>438
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>1_2D4
<400>221
atgatagaag tgaaacctat taacgcagag gatacttacg aacttcgaca caggatcctg 60
cgccctaatc agccgataga ggcatgcatg tatgaaagcg atctgctgcg gggctcgttc 120
catttgggcg ggttctatcg tggcaaattg atctcgattg cgagtttcca ccaagctgaa 180
cactcagacc tgcaagggca aaagcagtat caattacgag ggatggcgac cctcgaagga 240
taccgtgagc agaaggctgg ctcttcgctt attaagcacg ccgagcagct actacggaaa 300
aaaggggcag atatgctttg gtgtaatgca cgcacgtcag ccgccggtta ctataaaagg 360
cttggtttta gtgagcacgg cgaaattttc gaaaccccgc cggttgggcc gcacattctt 420
atgtacaaaa gaatcact 438
<210>222
<211>438
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>1_2F8
<400>222
atgctagaag tgaaacctat taacgcagag gatacttacg aacttcgaca caggatcctg 60
cgccctaatc agccgttaga ggcatgcatg tatgaaaccg atctgctgcg gggctcgttc 120
catttgggcg ggttctatcg tggcaaattg atctcgattg cgagtttcca ccaagctgaa 180
cattcagaac tggaagggca aaagcagtat caattacgag ggatggcgac tctcgaagga 240
taccgtgatc agaaggctgg ctcttcgctt attaggcacg ccgaggagat actacggaaa 300
agaggggcag atatgctttg gtgcaatgca cgcacgacag ccgccggtta ctataaaaag 360
cttggtttta gtgagcaggg cgaaatttac gacaccccgc cggttgggcc gcacattctt 420
atgtacaaaa aactcact 438
<210>223
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>1_2H8
<400>223
atgatagaag tgaaacctat taacgcagag gagacttacg aacttcgaca caagatcctg 60
cgccctaatc agccgttaga ggcatgcatg tatgaaaccg atctgctgcg gggcgcgttc 120
catttgggcg ggttctatcg tggcaaattg atctcgattg cgagtttcca ccaagctgac 180
cactcagaac tgcaagggca aaagcagtat caattacgag ggatggcgac cctcgaagga 240
taccgtgagc agaaggctgg ctctacgctt attaggcacg ccgagcagat actacggaaa 300
agaggggcag atctactttg gtgcaatgca cgcacgtcag ccgccggtta ctataaaaag 360
cttggtttta gtgagcacgg cgaaattttc gaaaccccgc cggttgggcc gcacattctt 420
atgtacaaaa gactcactta a 441
<210>224
<211>438
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>1_3A2
<400>224
atgatagaag tgaaacctat taacgcagag gatacttacg aacttcgaca caggatcctg 60
cgccctaatc agccgataga ggcatgcatg tatgaaagcg atctgctgcg gggcgcgttc 120
catttgggcg ggttctatcg tggcaaattg atctcgattg cgagtttcca ccaagctgaa 180
cactcagacc tgcaagggca aaagcagtat caattacgag ggatggcgac cctcgaagga 240
taccgtgagc agaaggctgg ctcttcgctt attaggcacg ccgaggagat actacggaaa 300
aaaggggcag atatgctttg gtgcaatgca cgcacgacag ccgccggtta ctataaaagg 360
cttggtttta gtgagcaggg cgaagttttc gacaccccgc cggttgggcc gcacattctt 420
atgtacaaaa gaatcact 438
<210>225
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>1_3D6
<400>225
atgatagagg tgaaaccgat taacgcagag gatacctatg aactaaggca tagaatactc 60
agaccaaacc agccgataga agtgtgtatg tatgaaaccg atttacttcg tggtgcattt 120
cacttaggcg gcttttacag gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagacc tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgagtcta attaaacacg ctgaacaaat tcttcgtaag 300
aggggggcgg acttgctttg gtgcaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agaggtattt gatacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggctcacata a 441
<210>226
<211>438
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>1_3F3
<400>226
atgatagaag tgaaacctat taacgcagag gagacttacg aacttcgaca gaggatcctg 60
cgccctaatc agccgataga ggcatgcatg tatgaaagcg atctgctgcg gggctcgttc 120
catttgggcg ggttctatcg tggccaattg atctcgattg cgagtttcca ccaagctgaa 180
cactcagaac tgcaagggca aaagcagtat caattacgag ggatggcgac cctcgaagga 240
taccgtgagc agaaggctgg ctctacgctt attaagcacg ccgaggagat actacggaaa 300
aaaggggcag atctgctttg gtgcaatgca cgcacgtcag ccgccggtta ctataaaagg 360
cttggtttta gtgagcacgg cgaaattttc gacaccccgc cggttgggcc gcacattctt 420
atgtacaaaa gaatcact 438
<210>227
<211>438
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>1_3H2
<400>227
atgatagaag tgaaacctat taacgcagag gatacttacg aacttcgaca caggatcctg 60
cgccctaatc agccgataga ggcatgcatg tatgaaaccg atctgctgcg gggcgcgttc 120
catttgggcg ggtactatcg tggccaattg atctcgattg cgagtttcca caaagctgaa 180
cactcagaac tgcaagggca aaagcagtat caattacgag ggatggcgac cctcgaagga 240
taccgtgagc agaaggctgg ctctacgctt attaagcacg ccgagcagct actacgggaa 300
aaaggggcag atatgctttg gtgcaatgca cgcacgtcag ccgccggtta ctataaaagg 360
cttggtttta gtgagcaggg cgaagttttc gacaccccgc cggttgggcc gcacattctt 420
atgtacaaaa aactcact 438
<210>228
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>1_4C5
<400>228
atgatagaag tgaaacctat taacgcagag gatacttacg aacttcgaca caagatcctg 60
cgccctaatc agccgataga ggcatgcatg tatgaaagcg atctgctgcg gggctcgttc 120
catttgggcg ggttctatcg tggcaaattg atctcgattg cgagtttcca caaagctgaa 180
cactcagacc tggaagggca aaaccagtat caattacgag ggatggcgac cctcgaagga 240
taccgtgagc agaaggctgg ctctacgctt attaggcacg ccgaggagat actacggaaa 300
agaggggcag atatgctttg gtgcaatgca cgcacgtcag cctccggtta ctataaaagg 360
cttggtttta gtgagcacgg cgaaattttc gacaccccgc cggttgggcc gcacattctt 420
atgtacaaaa gactcactta a 441
<210>229
<211>438
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>1_4D6
<400>229
atgctagaag tgaaacctat taacgcagag gatacttacg aacttcgaca caggatcctg 60
cgccctaatc agccgataga ggcatgcatg tatgaaaccg atctgctgcg gggctcgttc 120
catttgggcg ggttctatcg tggccaattg atctcgattg cgagtttcca caaagctgaa 180
cactcagacc tggaagggca aaagcagtat caattacgag ggatggcgac cctcgaagga 240
taccgtgagc agaaggctgg ctctacgctt attaggcacg ccgagcagat actacggaaa 300
agaggggcag atatgctctg gtgcaatgca cgcacgtcag ccgccggtta ctataaaagg 360
cttggtttta gtgagcaggg cgaagttttc gaaaccccgc cggttgggcc gcacattctt 420
atgtacaaaa gactcact 438
<210>230
<211>438
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>1_4H1
<400>230
atgatagaag tgaaacctat taacgcagag gatacttacg aacttcgaca caggatcctg 60
cgccctaatc agccgttaga ggcatgcatg tatgaaaccg atctgctgcg gggctcgttc 120
catttgggcg ggttctatcg tggcaaattg atctcgattg cgagtttcca ccaagctgaa 180
cactcagacc tgcaagggca aaagcagtat caattacgag ggatggcgac cctcgaagga 240
taccgtgagc agaaggctgg ctctacgctt attaggcacg ccgagcagct actacggaaa 300
agaggggcag atctgctttg gtgcaatgca cgcacgtcag cctccggtta ctataaaagg 360
cttggtttta gtgagcacgg cgaagttttc gacaccccgc cggttgggcc gcacattctt 420
atgtacaaaa gactcact 438
<210>231
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>1_5H5
<400>231
atgctagaag tgaaacctat taacgcagag gagacttacg aacttcgaca caagatcctg 60
cgccctaatc agccgttaga ggcatgcatg tatgaaagcg atctgctgcg gggctcgttc 120
catttgggcg ggtactatcg tggccaattg atctcgattg cgagtttcca ccaagctgaa 180
cactcagaac tggaagggca aaagcagtat caattacgag ggatggcgac cctcgaagga 240
ttccgtgagc agaaggctgg ctctacgctt attaagcacg ccgagcagat actacggaaa 300
agaggggcag atatgctttg gtgcaatgca cgcacgtcag ccgccggtta ctataaaaag 360
cttggtttta gtgagcacgg cgaaattttc gacaccccgc cggttgggcc gcacattctt 420
atgtacaaaa aactcactta a 441
<210>232
<211>438
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>1_6F12
<400>232
atgatagaag tgaaacctat taacgcagag gagacttacg aacttcgaca caggatcctg 60
cgccctaatc agccgataga ggcatgcatg tatgaaagcg atctgctgcg gggctcgttc 120
catttgggcg ggttctatcg tggcaaattg atctcgattg cgagtttcca ccaagctgaa 180
cactcagacc tagaagggca aaagcagtat caattacgag ggatggcgac cctcgaagga 240
taccgtgatc agaaggctgg ctctacgctt attaagcacg ccgaggagct actacggaaa 300
agaggggcag atatgctttg gtgcaatgca cgcacgtcag ccgccggtta ctataaaagg 360
cttggtttta gtgagcacgg cgaaatttac gaaaccccgc cggttgggcc gcacattctt 420
atgtacaaaa aaatcact 438
<210>233
<211>438
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>1_6H6
<400>233
atgatagaag tgaaacctat taacgcagag gatacttacg aacttcgaca caagatcctg 60
cgccctaatc agccgataga ggcatgcatg tatgaaagcg atctgctgcg gggctcgttc 120
catttgggcg ggttctatcg tggccaattg atctcgattg cgagtttcca ccaagctgaa 180
cactcagacc tggaagggca aaagcagtat caattacgag ggatggcgac cctcgaagga 240
taccgtgatc agaaggctgg ctcttcgctt attaagcacg ccgaggagat actacggaaa 300
agaggggcag atctgctttg gtgcaatgca cgcacgtcag ccgccggtta ctataaaagg 360
cttggtttta gtgagcaggg cgaaattttc gacaccccgc cggttgggcc gcacattctt 420
atgtacaaaa aaatcact 438
<210>234
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>3_11A10
<400>234
atgctagagg tgaaaccgat taacgcagag gatacctatg aactaaggca tagaatactc 60
agaccaaacc agccgataga agcgtgtatg tatgaaagcg atttacttcg tggtgcattt 120
cacttaggcg gctattacag gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagaac tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgagtcta gttaaacacg ctgaagaaat tcttcgtaag 300
aggggggcgg acttgctttg gtgtaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agagatattt gaaacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggatcacata a 441
<210>235
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>3_14F6
<400>235
atgctagagg tgaaaccgat taacgcagag gatacctatg aactaaggca tagaatactc 60
agaccaaacc agccgataga agcgtgtatg tatgaaagcg atttacttcg tggtgcattt 120
cacttaggcg gcttttacag gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagaac tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgagtcta attaaacacg ctgaagaaat tcttcgtaag 300
aggggggcgg acttgctttg gtgtaatgcg cggacgtccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agagatattt gaaacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggctcacata a 441
<210>236
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>3_15B2
<400>236
atgctagagg tgaaaccgat taacgcagag gatacctatg aactaaggca taaaatactc 60
agaccaaacc agccgttaga agtgtgtatg tatgaaaccg atttacttcg tggtgcattt 120
cacttaggcg gctattacgg gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagaac tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgagtcta attaaacacg ctgaagaaat tcttcgtaag 300
aggggggcgg acttgctttg gtgtaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agagatattt gaaacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggatcacata a 441
<210>237
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>3_6A10
<400>237
atgatagaag tgaaaccgat taacgcagag gatacctatg aactaaggca tagaatactc 60
agaccaaacc agccgataga agcgtgtatg tatgaaagcg atttacttcg tggtgcattt 120
cacttaggcg gctattacag gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagaac tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgagtcta attaaacacg ctgaagaaat tcttcgtaag 300
aggggggcgg acttgctttg gtgtaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agagatattt gaaacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggatcacata a 441
<210>238
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>3_6B1
<400>238
atgctagagg tgaaaccgat taacgcagag gatacctatg aactaaggca tagaatactc 60
agaccaaacc agccgataga agcgtgtatg tatgaaagcg atttacttcg tggtgcattt 120
cacttaggcg gctattacag gggcaaactg atttccatag cttcattcca ccaggccgag 180
cacccagaac tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgagtcta attaaacacg ctgaagaaat tcttcgtaag 300
aggggggcgg acttgctttg gtgtaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agaggtattt gaaacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggatcacata a 441
<210>239
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>3_7F9
<400>239
atgctagagg tgaaaccgat taacgcagag gatacctatg aactaaggca tagaatactc 60
agaccaaacc agccgataga agcgtgtatg tatgaaagcg atttacttcg tggtgcattt 120
cacttaggcg gctattacgg gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagacc tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgagtcta attaaacacg ctgaagaaat tcttcgtaag 300
aggggggcgg acttgctttg gtgtaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agagatattt gaaacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggatcacata a 441
<210>240
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>3_8G11
<400>240
atgctagagg tgaaaccgat taacgcagag gatacctatg aactaaggca tagaatactc 60
agacccaacc agccgataga agtgtgtatg tatgaaagcg atttacttcg tggtgcattt 120
cacttaggcg gctattacag gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagaac tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgagtcta attaaacacg ctgaagaaat tcttcgtaag 300
aggggggcgg acttgctttg gtgtaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agagatattt gaaacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggatcacata a 441
<210>241
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>4_1B10
<400>241
atgatagaag tgaaacctat taacgcagag gatacctatg aactaaggca tagaatactc 60
agaccaaacc agccgataga agtgtgtatg tatgaaaccg atttacttcg tggtgcattt 120
cacttaggcg gcttttacgg gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagacc tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgatc agaaagcggg atcgagtcta attagacacg ctgaacaaat tcttcgtaag 300
aggggggcgg acatgctttg gtgcaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agagatattt gaaacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggatcacata a 441
<210>242
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>5_2B3
<400>242
atgatagaag tgaaacctat taacgcagag gatacctatg aactaaggca tagaatactc 60
agaccaaacc agccgttaga agtgtgtatg tatgaaaccg atttacttcg tggtgcattt 120
cacttaggcg gcttttacgg gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagacc tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgatc agaaagcggg atcgagtcta attagacacg ctgaacaaat tcttcgtaag 300
aggggggcgg acatgctttg gtgtaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agagatattt gaaacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggatcacata a 441
<210>243
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>5_2D9
<220>
<221>混杂的特征
<222>9,76,98
<223>n=A,T,C或G
<400>243
atgctagang tgaaaccgat taacgcagag gatacctatg aactaaggca taaaatactc 60
agaccaaacc agccgntaga agtgtgtatg tatgaaancg atttacttcg tggtgcattt 120
cacttaggcg gcttttacag gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagacc tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgatc agaaagcggg atcgagtcta attaaacacg ctgaacaaat tcttcgtgag 300
aggggggcgg acatgctttg gtgcaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agaggtattt gacacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggctcacata a 441
<210>244
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>5_2F10
<400>244
atgctagaag tgaaacctat taacgcagag gatacctatg aactaaggca taaaatactc 60
agaccaaacc agccgataga agtgtgtatg tatgaaaccg atttacttcg tggtgcattt 120
cacttaggcg gcttttacgg gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagacc tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgatc agaaagcggg atcgagtcta attagacacg ctgaacaaat tcttcgtaag 300
aggggggcgg acatgctttg gtgcaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agagatattt gaaacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggctcacata a 441
<210>245
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>6_1A11
<400>245
atgctagagg tgaaaccgat taacgcagag gatacctatg aactaaggca taaaatactc 60
agaccaaacc agccgttaga agtgtgtatg tatgaaaccg atttacttcg tggtgcattt 120
cacttaggcg gcttttacag gggcaaactg atttccatag cgtcattcca ccaggccgag 180
cactcagacc tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgatc agaaagcggg atcgagtcta attagacacg ctgaacaaat tcttcgtaag 300
aggggggcgg acatgctttg gtgcaatgcg cggacatccg cctcaggcta ctacagaaag 360
ttaggcttca gcgagcaggg agaggtattt gaaacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggctcacata a 441
<210>246
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>6_1D5
<400>246
atgatagagg tgaaaccgat taacgcagag gatacctatg aactaaggca taaaatactc 60
agaccaaacc agccgttaga agtgtgtatg tatgaaaccg atttacttcg tggtgcattt 120
cacttaggcg gcttttacag gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagacc tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgatc agaaagcggg atcgagtcta attagacacg ctgaacaaat tcttcgtaag 300
aggggggcgg acatgctttg gtgcaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg ggaggtattt gaaacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggatcacata a 441
<210>247
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>6_1F11
<400>247
atgatagagg tgaaaccgat taacgcagag gatacctatg aactaaggca taaaatactc 60
agaccaaacc agccgttaga agtgtgtatg tatgaaaccg atttacttcg tggtgcattt 120
cacttaggcg gcttttacag gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagacc tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgagtcta attagacacg ctgaacaaat tcttcgtaag 300
aggggggcgg acatgctttg gtgcaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agaggtattt gaaacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggctcacata a 441
<210>248
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>6_1F1
<400>248
atgatagagg tgaaaccgat taacgcagag gatacctatg aactaaggca taaaatactc 60
agaccaaacc agccgttaga agtgtgtatg tatgaaaccg atttacttcg tggtgcattt 120
cacttaggcg gcttttacag gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagacc tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgagtcta attagacacg ctgaacaaat tcttcgtaag 300
aggggggcgg acatgctttg gtgcaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agaggtattt gaaacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggctcacata a 441
<210>249
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>6_1H10
<400>249
atgctagagg tgaaaccgat taacgcagag gatacctatg aactaaggca taaaatactc 60
agaccaaacc agccgttaga agtgtgtatg tatgaaaccg atttacttcg tggtgcattt 120
cacttaggcg gcttttacgg gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcggacc tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgatc agaaagcggg atcgagtcta attagacacg ctgaagaaat tcttcgtaag 300
aggggggcgg acatgctttg gtgcaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agaggtattt gacacgccgc cagtaggacc tcacatcctg 420
atgtataaaa agatcacata a 441
<210>250
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>6_1H4
<400>250
atgctagaag tgaaaccgat taacgcagag gatacctatg aactaaggca taaaatactc 60
agaccaaacc agccgttaga agtgtgtatg tatgaaaccg atttacttcg tggtgcattt 120
cacttaggcg gcttttacgg gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagacc tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgatc agaaagcggg atcgactcta attaaacacg ctgaacaaat tcttcgtaag 300
aggggggcgg acatgctttg gtgcaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agaggtattt gaaacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggctcacata a 441
<210>251
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>8_1F8
<400>251
atgatagagg tgaaaccgat taacgcagag gatacctatg aactaaggca tagaatactc 60
agaccaaacc agccgttaga agtgtgtatg tatgaaaccg atttacttcg tggtgcattt 120
cacttaggcg gcttttacag gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagacc tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgagtcta attaaacacg ctgaagaaat tcttcgtaag 300
aggggggcgg acttgctttg gtgtaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agagatattt gatacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggatcacata a 441
<210>252
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>8_1G2
<400>252
atgatagagg tgaaaccgat taacgcagag gatacctatg aactaaggca tagagtactc 60
agaccaaacc agccgttaga agtgtgtatg tatgaaaccg atttacttcg tggtgcattt 120
cacttaggcg gctattacag gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagaac tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgagtcta attaaacacg ctgaagaaat tcttcgtaag 300
aggggggcgg acttgctttg gtgcaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agaggtattt gagacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggctcacgta a 441
<210>253
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>8_1G3
<400>253
atgctagagg tgaaaccgat taacgcagag gatacttacg aactaaggca taaaatactc 60
agaccaaacc agccgataga agtgtgtatg tatgaaaccg atttacttcg tggtgcattt 120
cacttaggcg gctattacag gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagaac tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgagtcta attagacacg ctgaagaaat tcttcgtaag 300
aggggggcgg acttgctttg gtgtaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agagatattt gatacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggatcacgta a 441
<210>254
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>8_1H7
<400>254
atgctagagg tgaaaccgat taacgcagag gatacctatg aactaaggca tagaatactc 60
agaccaaacc agccgataga agtgtgtatg tatgaaaccg atttacttcg tggtgcattt 120
cacttaggcg gcttttacag gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagaac tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgagtcta attaaacacg ctgaagaaat tcttcgtaag 300
aggggggcgg acatgctttg gtgcaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agagatattt gaaacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggctcacata a 441
<210>255
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>8_1H9
<400>255
atgctagagg tgaaaccgat taacgcagag gatacctatg aactaaggca taaaatactc 60
agaccaaacc agccgttaga agtgtgtatg tatgaaaccg atttacttcg tggtgcattt 120
cacttaggcg gctattacag gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagacc tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgagtcta attagacacg ctgaagaaat tcttcgtaag 300
aggggggcgg acttgctttg gtgtaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agaggtattt gatacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggctcacata a 441
<210>256
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>GAT1_21F12
<400>256
atgattgaag tcaaacctat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggcg gatattaccg gggcaagctg atcagcatcg cttcctttca taatgccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac gcttgaagga 240
taccgtgagc aaaaagcggg aagcacgctc atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcgg accttttatg gtgcaacgcc aggacatctg tgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgatcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>257
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>GAT1_24G3
<400>257
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cctcctttca tcaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaaggg 240
taccgcgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaatgcc aggacatttg tgagcggtta ctatgaaaag 360
ctcggtttca gcgaacaggg cgaagtctac gacataccgc cgatcggacc ttatattttg 420
atgtattaga aattgacata a 441
<210>258
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>GAT1_29G1
<400>258
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcgg gggtacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cttcctttca tcaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaaggg 240
taccgcgagc aaaaagcggg tagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacatctg tgagcgggta ctataaaaag 360
ctcggcttca gcgaacaagg cggggtctgc gatataccgc cgatcggac ctcatattttg 420
atgtataaga aattggcata a 441
<210>259
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>GAT1_32G1
<400>259
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcatactc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cttcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaaggg 240
taccgcgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcag accttttatg gtgcaacgcc aggacatctg tgagcggcta ctatgaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgatcggacc tcatattttg 420
atgtataaga aattgacata a 441
<210>260
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>GAT2_15G8
<400>260
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgctgga agcatgcaag tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cttcctttca taatgccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac gcttgaaggg 240
taccgcgagc aaaaagcggg aagcacgctc atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcag accttttatg gtgcaacgcc aggacatctg tgagcgggta ctataaaaag 360
ctcggcttca gcgaacaggg cgaagtctac gacataccgc cgatcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>261
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>GAT2_19H8
<400>261
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcatactc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cttcctttca tcaagccgaa 180
catccagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaaggg 240
taccgcgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aaaggcgcag accttttatg gtgcaacgcc aggacatctg tgagcggcta ctatgaaaag 360
ctcggcttca gcgaacaggg cgaagtctgc gacataccgc cgatcggacc tcatattttg 420
atgtataaga aattgacata a 441
<210>262
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>GAT2_21F1
<400>262
atgattgaag tcaaaccaat aaacgcggaa gatacgtatg agatcaggca ccgcattctc 60
cggccgaatc agccgcttga agcatgtatg tatgaaaccg atttgctcgg gggcacgttt 120
cacctcggtg gatattaccg gggcaagctg atcagcatcg cttcctttca tcaagccgaa 180
cattcagagc ttgaaggcca aaaacagtat cagctgagag ggatggcgac acttgaagga 240
taccgtgagc aaaaagcggg cagtacgctt atccgccatg ccgaagagct tcttcggaaa 300
aagggggcag accttttatg gtgcaacgcc aggacatctg tgagcgggta ctataaaaag 360
ctcggcttca gcgaacaagg cggggtctac gatataccgc cgatcggacc tcatattttg 420
atgtataaga aattgacgta a 441
<210>263
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>13_10F6
<400>263
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>264
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>13_12G6
<400>264
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Val Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Arg Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Thr Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>265
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>14_2A5
<400>265
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Ser Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe Asn Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>266
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>14_2C1
<400>266
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Val Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Thr Pro Pro Thr Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>267
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>14_2F11
<400>267
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Val Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Ala
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Thr Pro Pro Ala Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>268
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>嵌合体
<400>268
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>269
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>10_12D7
<400>269
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Leu His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Glu
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Thr Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>270
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>10_15F4
<400>270
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Val Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Glu
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Gly
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>271
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>10_17D1
<400>271
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>272
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>10_17F6
<400>272
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Val Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Glu
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>273
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>10_18G9
<400>273
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Val Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Glu
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Gly
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>274
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>10_1H3
<400>274
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Val Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Arg Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Thr Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>275
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>10_20D10
<400>275
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Leu His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Glu
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Gly
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>276
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>10_23F2
<400>276
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Val Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>277
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>10_2B8
<400>277
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Glu
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>278
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>10_2C7
<400>278
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>279
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>10_3G5
<400>279
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Val Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Thr Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>280
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>10_4H7
<400>280
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Val Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Thr Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>281
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>10_6D11
<400>281
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Leu His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Val Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>282
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>10_8C6
<400>282
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Gly
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>283
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>11C3
<400>283
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Gln Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Val Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Gly
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>284
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>11G3
<400>284
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Gln Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Glu Lys Leu Gly Phe Ser Glu Gln Gly Gly
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Ala
145
<210>285
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>11H3
<400>285
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Ala Phe His Leu Gly Gly Tyr Tyr Gln Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Lys Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Val Arg Gly Tyr Tyr Glu Lys Leu Gly Phe Ser Glu Gln Gly Gly
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>286
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>12_1F9
<400>286
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val His Asp Ile Pro Pro Thr Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>287
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>12_2G9
<400>287
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Val Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>288
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>12_3F1
<400>288
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Gly
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>289
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>12_5C10
<400>289
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Glu
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ala Pro Pro Thr Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>290
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>12_6A10
<400>290
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Val Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Gly
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>291
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>12_6D1
<400>291
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Glu
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Gly
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>292
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>12_6F9
<400>292
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Thr Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>293
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>12_6H6
<400>293
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Val Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Ala
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Thr Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>294
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>12_7D6
<400>294
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Gly
115 120 125
Val Tyr Asp Ile Pro Pro Thr Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>295
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>12_7G11
<400>295
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>296
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>12F5
<400>296
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Gln Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Lys Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Val Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Gly
115 120 125
Ile Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>297
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>12G7
<400>297
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Gln Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Lys Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Val Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>298
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>1_2H6
<400>298
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Gly
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>299
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>13_12G12
<400>299
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe Asn Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Glu
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met His Lys Lys
130 135 140
Leu Thr
145
<210>300
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>13_6D10
<400>300
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Ser Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe Asn Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>301
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>13_7A7
<400>301
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Ser Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Glu
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>302
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>13_7B12
<400>302
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Ser Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Thr Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>303
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>13_7C1
<400>303
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Arg Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Thr Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>304
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>13_8G6
<400>304
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Ser Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe Asn Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Gly
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>305
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>13_9F6
<400>305
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Glu
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>306
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>14_10C9
<400>306
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Glu
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>307
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>14_10H3
<400>307
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Val Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Glu
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>308
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>14_10H9
<400>308
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Val Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>309
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>14_11C2
<400>309
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Ser Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Val Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Glu
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Ala
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Thr Pro Pro Thr Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>310
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>14_12D8
<400>310
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Val Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Ala
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Arg Glu Gln Gly Gly
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>311
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>14_12H6
<400>311
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Glu
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Thr Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>312
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>14_2B6
<400>312
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe Asn Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Gly
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>313
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>14_2G11
<400>313
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Val Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Thr Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>314
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>14_3B2
<400>314
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Val Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Ala
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Gly
115 120 125
Val Tyr Asp Ile Pro Pro Ala Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>315
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>14_4H8
<400>315
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Ser Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>316
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>14_6A8
<400>316
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Val Ser Ile Ala Ser Phe Asn Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Thr Pro Pro Val Gly Pro His Val Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>317
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>14_6B10
<400>317
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Gly
115 120 125
Val Tyr Asp Met Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>318
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>14_6D4
<400>318
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe Asn Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Ala
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>319
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>14_7A11
<400>319
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Val Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Leu Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Thr Pro Pro Thr Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>320
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>14_7A1
<400>320
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Val Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Glu
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Thr Pro Pro Ala Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>321
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>14_7A9
<400>321
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Val Ser Ile Ala Ser Phe His Gln Ala Lys His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>322
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>14_7G1
<400>322
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe Asn Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Glu
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Ala
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>323
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>14_7H9
<400>323
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Val Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>324
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>14_8F7
<400>324
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Val Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Glu
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Ala
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Thr Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>325
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>15_10C2
<400>325
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Val Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Thr Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Ile Pro Pro Thr Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>326
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>15_10D6
<400>326
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Val Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Glu
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>327
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>15_11F9
<400>327
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Val Ser Ile Ala Ser Phe Asn Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Arg Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Thr Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>328
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>15_11H3
<400>328
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Ala
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Thr Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>329
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>15_12A8
<400>329
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Ala
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Thr Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>330
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>15_12D6
<400>330
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Val Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>331
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>15_12D8
<400>331
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Val Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Lys
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>332
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>15_12D9
<400>332
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Val Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Glu
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>333
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>15_3F10
<400>333
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Val Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Thr Pro Pro Ala Gly Pro His Ile Leu Met Tyr Thr Lys
130 135 140
Leu Thr
145
<210>334
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>15_3G11
<400>334
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Val Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Glu
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>335
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>15_4F11
<400>335
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Lys Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Val Ser Ile Ala Ser Phe Asn Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Ala
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Thr Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>336
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>15_4H3
<400>336
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Val Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Glu
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Thr Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>337
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>15_6D3
<400>337
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Glu
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Thr Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>338
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>15_6G11
<400>338
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Val Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Glu
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Lys
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>339
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>15_9F6
<400>339
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Glu
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Arg Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>340
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>15F5
<400>340
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Lys Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Glu Glu Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg Tyr Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Val Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>341
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>16A1
<400>341
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Leu His Leu Gly Gly Tyr Tyr Gln Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Lys Ala Glu His Ser Gly Leu
50 55 60
Glu Gly Glu Glu Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Val Ser Gly Tyr Tyr Glu Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>342
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>16H3
<400>342
Met Ile Asp Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Gln Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Val Ser Gly Tyr Tyr Glu Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>343
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>17C12
<400>343
Met Ile Glu Val Lys Pro Ile Ser Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Ala Phe His Leu Gly Gly Tyr Tyr Gln Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Val Ser Gly Tyr Tyr Glu Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>344
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>18D6
<400>344
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Lys Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Glu Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Ala
145
<210>345
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>19C6
<400>345
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Cys Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Val Arg Gly Tyr Tyr Glu Lys Leu Gly Phe Ser Glu Gln Gly Gly
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Ala
145
<210>346
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>19D5
<400>346
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Cys Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Gln Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Lys Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Val Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>347
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>20A12
<400>347
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Gln Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Asn Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Val Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Val Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Gly
115 120 125
Ile Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Ala
145
<210>348
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>20F2
<400>348
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Val Ser Gly Tyr Tyr Glu Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>349
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>21E11
<400>349
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Ala Phe His Leu Gly Gly Tyr Tyr Gln Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Val Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>350
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>23H11
<400>350
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Gln Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Lys Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Glu Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Ala
145
<210>351
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>24C1
<400>351
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Asp
35 40 45
Arg Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Val Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>352
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>24C6
<400>352
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Ile
100 105 110
Ser Val Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Gly
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Ala
145
<210>353
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>24E7
<400>353
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Asn Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Glu Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Ala
145
<210>354
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>2_8C3
<400>354
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Asp
35 40 45
Arg Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Glu Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>355
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>2H3
<400>355
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Gln Gly
35 40 45
Lys Leu Ile Ser Thr Ala Ser Phe His Gln Ala Gly His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Arg Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Ile
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Gly
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>356
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>30G8
<400>356
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Phe Glu
20 25 30
Thr Asp Leu Leu Gly Gly Ala Phe His Leu Gly Gly Tyr Tyr Gln Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Val Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>357
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>3B_10C4
<400>357
Met Ile Glu Val Arg Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu GLu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ala Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>358
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>3B_10G7
<400>358
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Gly
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>359
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>3B_12B1
<400>359
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>360
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>3B_12D10
<400>360
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Pro Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Ile
100 105 110
Ser Ala Ser Gly Tyr Tyr Glu Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>361
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>3B_2E5
<400>361
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Glu Lys Leu Gly Phe Ser Lys Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>362
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>3C_10H3
<400>362
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Ile
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Gly
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>363
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>3C_12H10
<400>363
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Gly Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Glu Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>364
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>3C_9H8
<400>364
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr TyrGlu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Gln Asp
35 40 45
Arg Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg Tyr Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Ile
100 105 110
Ser Ala Ser Gly Tyr Tyr Glu Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>365
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>4A_1B11
<400>365
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Tur Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Glu Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>366
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>4A_1C2
<400>366
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Glu
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>367
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>4B_13E1
<400>367
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Glu
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Ile
100 105 110
Ser Ala Ser Gly Tyr Tyr Glu Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>368
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>4B_13G10
<400>368
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Gly
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro Tyr Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>369
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>4B_16E1
<400>369
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Gly
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>370
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>4B_17A1
<400>370
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Glu
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Glu Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>371
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>4B_18F11
<400>371
Met Ile Glu Val Asn Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Ser His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Asn Ala Glu His Ser Glu Leu
50 55 60
Asp Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Val Ser Gly Tyr Tyr Glu Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Ser Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>372
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>4B_19C8
<400>372
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Gly
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Ala
145
<210>373
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>4B_1G4
<400>373
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ser Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Leu Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Ile
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>374
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>4B_21C6
<400>374
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Glu
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Ile
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Gly
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>375
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>4B_2H7
<400>375
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Gly
115 120 125
Val Tyr Gly Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>376
<211>146
<212>PRT
<213>人工序列
<220>
<222>合成的蛋白质序列
<220>
<223>4B_2H8
<400>376
Met Ile Glu Ala Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>377
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>4B_6D8
<400>377
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu His Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>378
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>4B_7E8
<400>378
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Val Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>379
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>4C_8C9
<400>379
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Glu Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>380
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>4H1
<400>380
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Ala Phe His Leu Gly Gly Tyr Tyr Gln Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Val His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Val Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Gly
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>381
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>6_14D10
<400>381
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly His Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Glu
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Gly
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>382
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>6_15G7
<400>382
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>383
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>6_16A5
<400>383
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Gly
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>384
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>6_16F5
<400>384
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Val His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Gly
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>385
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>6_17C5
<400>385
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Ala Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Ash Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Val Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>386
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>6_18C7
<400>386
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Arg Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Ile
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>387
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>6_18D7
<220>
<221>变体
<222>17
<223>Xaa=任何氨基酸
<400>387
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
Xaa Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Gly
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>388
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>6_19A10
<400>388
Met Ile Glu Ala Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Thr Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>389
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>6_19B6
<400>389
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>390
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>6_19C3
<400>390
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>391
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>6_19C8
<400>391
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Leu His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg Gln Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Gly
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Glu
130 135 140
Leu Thr
145
<210>392
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>6_20A7
<400>392
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Asp Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Glu
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>393
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>6_20A9
<400>393
Met Ile Glu Val Lys Pro Ile Asn Ala Gly Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Gly
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>394
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>6_20H5
<400>394
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>395
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>6_21F4
<400>395
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Val Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>396
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>6_22C9
<400>396
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Arg Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Gly Leu
50 55 60
Glu Gly Lys Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Glu
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Gly
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>397
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>6_22D9
<400>397
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Glu Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>398
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>6_22H9
<400>398
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Asp Glu
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>399
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>6_23H3
<400>399
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Gly
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu Gln Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Gly
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>400
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>6_23H7
<400>400
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Gly
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>401
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>6_2H1
<400>401
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Pro Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr TyrLys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>402
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>6_3D6
<400>402
Met Ile Glu Ile Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>403
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>6_3G3
<400>403
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>404
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>6_3H2
<400>404
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Glu
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>405
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>6_4A10
<400>405
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>406
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>6_4B1
<400>406
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Gly Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Glu Lys Leu Gly Phe Ser Gly Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>407
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>6_5D11
<400>407
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Glu
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>408
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>6_5F11
<400>408
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Glu
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val His Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>409
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>6_5G9
<400>409
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Glu
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Ile
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Gly
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>410
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>6_6D5
<400>410
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Ala Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Gly
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>411
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>6_7D1
<400>411
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Gly
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>412
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>6_8H3
<400>412
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Gly
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>413
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>6_9G11
<400>413
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Leu His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>414
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>6F1
<400>414
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Val Cys Ile Ala Ser Phe His Lys Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Asp Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Val Ser Gly Tyr Tyr Glu Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>415
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>7_1C4
<400>415
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Glu
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Gly
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>416
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>7_2A10
<400>416
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Gly
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>417
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>7_2A11
<400>417
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Gly
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>418
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>7_2D7
<400>418
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>419
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>7_5C7
<400>419
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Val Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Gly
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>420
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>7_9C9
<400>420
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>421
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>9_13F10
<400>421
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Val Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Glu
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Thr Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>422
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>9_13F1
<400>422
Met Ile Glu Ala Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Val Ser Ile Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Glu
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>423
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>9_15D5
<400>423
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Asp Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>424
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>9_15D8
<400>424
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Val Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Ala
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>425
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>9_15H3
<400>425
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Met Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Glu
65 70 75 80
Tyr His Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asn Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>426
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>9_18H2
<400>426
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Val Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>427
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>9_20F12
<400>427
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Glu Leu Val Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Gly
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>428
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>9_21C8
<400>428
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Asp Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>429
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>9_22B1
<400>429
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Val Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Leu Pro Pro Thr Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>430
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>9_23A10
<400>430
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Leu His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Val Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Gly Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Gly
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>431
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>9_24F6
<400>431
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Ala
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Thr Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>432
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>9_4H10
<400>432
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Leu His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Ile Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>433
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>9_4H8
<400>433
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe Asn Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>434
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>9_8H1
<400>434
Met Ile Glu Val Lys Pro Ile Thr Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Thr Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>435
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>9_9H7
<400>435
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Ala Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Ser Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Glu
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>436
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>9C6
<400>436
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Gln Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Asn Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Val Ser Gly Tyr Tyr Glu Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Ala
145
<210>437
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>9H11
<400>437
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Lys Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Glu Glu Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Val Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>438
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>0_4B10
<400>438
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Asp Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Asp Gln Lys Ala Gly Ser Thr Leu Ile Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Thr Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Leu Thr
145
<210>439
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>0_5B11
<400>439
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Asp Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Asp Gln Lys Ala Gly Ser Thr Leu Ile Lys His Ala Glu Gln
85 90 95
Leu Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Ile Thr
145
<210>440
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>0_5B3
<400>440
Met Leu Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Asp Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Gln
85 90 95
Leu Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>441
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>0_5B4
<400>441
Met Leu Glu Val Lys Leu Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Asp Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Phe Arg Asp Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asn Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>442
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>0_5B8
<400>442
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Asp Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Asp Gln Lys Ala Gly Ser Ser Leu Ile Arg His Ala Glu Gln
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Leu Thr
145
<210>443
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>0_5C4
<400>443
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Gly Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Ile Ile Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>444
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>0_5D11
<400>444
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Asp Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Gln
85 90 95
Leu Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Arg Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Leu Thr
145
<210>445
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>0_5D3
<400>445
Met Leu Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>446
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>0_5D7
<400>446
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Glu Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Asp Gln Lys Ala Gly Ser Ser Leu Ile Arg His Ala Glu Gln
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asn Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Thr Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>447
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>0_6B4
<400>447
Met Leu Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Leu His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Asp Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Phe Arg Asp Gln Lys Ala Gly Ser Ser Leu Ile Arg His Ala Glu Gln
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Lys
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>448
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>0_6D10
<400>448
Met Leu Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Leu Glu Val Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Asp Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Asp Gln Lys Ala Gly Ser Ser Leu Ile Arg His Ala Glu Gln
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Leu Thr
145
<210>449
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>0_6D11
<400>449
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Asp Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Phe Arg Asp Gln Lys Ala Gly Ser Ser Leu Ile Arg His Ala Glu Gln
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>450
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>0_6F2
<400>450
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Phe Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Gln
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>451
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>0_6H9
<400>451
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Asp Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Lys Gly Ala Asn Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Leu Thr
145
<210>452
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>10_4C10
<220>
<221>变体
<222>45,105
<223>Xaa=任何氨基酸
<400>452
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Leu Glu Val Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Xaa Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Asp Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Gln
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Xaa Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Leu Thr
145
<210>453
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>10_4D5
<400>453
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Val Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Asp Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Gln
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>454
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>10_4F2
<400>454
Met Leu Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Arg His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Leu Thr
145
<210>455
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>10_4F9
<400>455
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Val Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Phe Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Arg His Ala Glu Gln
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Leu Thr
145
<210>456
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>10_4G5
<400>456
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Asp Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Asp Gln Lys Ala Gly Ser Ser Leu Ile Arg His Ala Glu Gln
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Leu Thr
145
<210>457
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>10_4H4
<400>457
Met Leu Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Leu Glu Val Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>458
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>11_3A11
<400>458
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Ile Glu Val Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Asp Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Asp Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Gln
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Leu Thr
145
<210>459
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>11_3B1
<400>459
Met Leu Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Asp Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Phe Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Arg Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Leu Thr
145
<210>460
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>11_3B5
<400>460
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Asp Gln Lys Ala Gly Ser Ser Leu Ile Arg His Ala Glu Gln
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>461
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>11_3C12
<400>461
Met Ile Glu Val Lys Pro Ile Asr Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Val Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Asp Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Asp Gln Lys Ala Gly Ser Ser Leu Ile Arg His Ala Glu Gln
85 90 95
Leu Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>462
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>11_3C3
<400>462
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Leu His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>463
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>11_3C6
<400>463
Met Leu Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Asp Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>464
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>11_3D6
<400>464
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Val Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Asp Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Gln
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Leu Thr
145
<210>465
<211>146
<212>PRT
<213>人工序列
<220>
<222>合成的蛋白质序列
<220>
<223>1_1G12
<400>465
Met Leu Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Val Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Asp Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Leu Thr
145
<210>466
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>1_1H1
<400>466
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Glu Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ser Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Gln Leu Ile Ser Ile Ala Ser Phe His Lys Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Phe Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Arg His Ala Glu Glu
85 90 95
Ile Leu Arg Asn Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Thr Ala Ser Gly Tyr Tyr Lys Arg Leu Gly Phe Ser Glu His Gly Glu
115 120 125
Val Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>467
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>1_1H2
<400>467
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ser Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Phe Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Arg His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Thr Ala Ala Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>468
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>1_1H5
<400>468
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ser Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Asp Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Asp Gln Lys Ala Gly Ser Ser Leu Ile Arg His Ala Glu Gln
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Thr Ala Ala Gly Tyr Tyr Lys Arg Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>469
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>1_2A12
<400>469
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ser Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu Gln Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Asp Gln Lys Ala Gly Ser Thr Leu Ile Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ala Gly Tyr Tyr Lys Arg Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Leu Thr
145
<210>470
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>1_2B6
<400>470
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Glu Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ser Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Phe Arg Asp Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Leu Thr
145
<210>471
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>1_2C4
<400>471
Met Leu Glu Val Lys Pro Ile Asn Ala Glu Glu Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ser Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Gln Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Asp Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Lys His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Thr Ala Ala Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Ile Thr
145
<210>472
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>1_2D2
<400>472
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Arg Ser Ala Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Lys Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Asp Gln Lys Ala Gly Ser Ser Leu Ile Arg His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ala Gly Tyr Tyr Lys Arg Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>473
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>1_2D4
<400>473
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ser Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Asp Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Gln
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ala Gly Tyr Tyr Lys Arg Leu Gly Phe Ser Glu His Gly Glu
115 120 125
Ile Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>474
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>1_2F8
<400>474
Met Leu Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ser Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Asp Gln Lys Ala Gly Ser Ser Leu Ile Arg His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Thr Ala Ala Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Tyr Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>475
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>1_2H8
<400>475
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Glu Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Asp His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Gln
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ala Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu His Gly Glu
115 120 125
Ile Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Leu Thr
145
<210>476
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>1_3A2
<400>476
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Asp Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Arg His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Lys Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Thr Ala Ala Gly Tyr Tyr Lys Arg Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>477
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>1_3D6
<400>477
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Gln Gly Ser Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Gln Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Asp Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Phe Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ala Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu His Gly Glu
115 120 125
Ile Phe Asp Thr Pro Pro Ala Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>478
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>1_3F3
<400>478
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Glu Thr Tyr Glu Leu Arg
1 5 10 15
Gln Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ser Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Gln Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ala Gly Tyr Tyr Lys Arg Leu Gly Phe Ser Glu His Gly Glu
115 120 125
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>479
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>1_3H2
<400>479
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Gln Leu Ile Ser Ile Ala Ser Phe His Lys Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Lys His Ala Glu Gln
85 90 95
Leu Leu Arg Glu Lys Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ala Gly Tyr Tyr Lys Arg Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>480
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>1_4C5
<400>480
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ser Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Lys Ala Glu His Ser Asp Leu
50 55 60
Glu Gly Gln Asn Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Arg Leu Gly Phe Ser Glu His Gly Glu
115 120 125
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Leu Thr
145
<210>481
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>1_4D6
<400>481
Met Leu Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ser Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Gln Leu Ile Ser Ile Ala Ser Phe His Lys Ala Glu His Ser Asp Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Gln
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ala Gly Tyr Tyr Lys Arg Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Leu Thr
145
<210>482
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>1_4H1
<400>482
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ser Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Asp Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Gln
85 90 95
Leu Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Arg Leu Gly Phe Ser Glu His Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Leu Thr
145
<210>483
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>1_5H5
<400>483
Met Leu Glu Val Lys Pro Ile Asn Ala Glu Glu Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ser Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Gln Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Phe Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Lys His Ala Glu Gln
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ala Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu His Gly Glu
115 120 125
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>484
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>1_6F12
<400>484
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Glu Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ser Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Asp Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Asp Gln Lys Ala Gly Ser Thr Leu Ile Lys His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ala Gly Tyr Tyr Lys Arg Leu Gly Phe Ser Glu His Gly Glu
115 120 125
Ile Tyr Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Ile Thr
145
<210>485
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>1_6H6
<400>485
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ser Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Gln Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Asp Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Asp Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ala Gly Tyr Tyr Lys Arg Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Ile Thr
145
<210>486
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>3_11Al0
<400>486
Met Leu Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>487
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>3_14F6
<400>487
Met Leu Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Leu Thr
145
<210>488
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>3_15B2
<400>488
Met Leu Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Leu Glu Val Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>489
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>3_6A10
<400>489
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>490
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>3_6B1
<400>490
Met Leu Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>491
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>3_7F9
<400>491
Met Leu Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Asp Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>492
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>3_8G11
<400>492
Met Leu Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Val Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>493
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>4_1B10
<400>493
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Val Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Asp Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Asp Gln Lys Ala Gly Ser Ser Leu Ile Arg His Ala Glu Gln
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>494
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>5_2B3
<400>494
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Val Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Asp Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Asp Gln Lys Ala Gly Ser Ser Leu Ile Arg His Ala Glu Gln
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>495
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>5_2D9
<220>
<221>变体
<222>3,26,33
<223>Xaa=任何氨基酸
<400>495
Met Leu Xaa Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Xaa Glu Val Cys Met Tyr Glu
20 25 30
Xaa Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Asp Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Asp Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Gln
85 90 95
Ile Leu Arg Glu Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Leu Thr
145
<210>496
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>5_2F10
<400>496
Met Leu Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Ile Glu Val Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Asp Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Asp Gln Lys Ala Gly Ser Ser Leu Ile Arg His Ala Glu Gln
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Leu Thr
145
<210>497
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>6_1A11
<400>497
Met Leu Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Leu Glu Val Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Asp Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Asp Gln Lys Ala Gly Ser Ser Leu Ile Arg His Ala Glu Gln
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Leu Thr
145
<210>498
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>6_1D5
<400>498
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Leu Glu Val Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Asp Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Asp Gln Lys Ala Gly Ser Ser Leu Ile Arg His Ala Glu Gln
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>499
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>6_1F11
<400>499
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Leu Glu Val Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Asp Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Arg His Ala Glu Gln
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Leu Thr
145
<210>500
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>6_1F1
<400>500
Met Leu Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Leu Glu Val Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Asp Gln Lys Ala Gly Ser Ser Leu Ile Arg His Ala Glu Gln
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Leu Thr
145
<210>501
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>6_1H10
<400>501
Met Leu Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Leu Glu Val Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Asp Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Asp Gln Lys Ala Gly Ser Ser Leu Ile Arg His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Ile Thr
145
<210>502
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>6_1H4
<400>502
Met Leu Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Leu Glu Val Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Asp Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Asp Gln Lys Ala Gly Ser Thr Leu Ile Lys His Ala Glu Gln
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tvr Lys Arg
130 135 140
Leu Thr
145
<210>503
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>8_1F8
<400>503
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Val Cys Met Tvr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Asp Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>504
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>8_1G2
<400>504
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Leu Glu Val Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Leu Thr
145
<210>505
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>8_1G3
<400>505
Met Leu Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Ile Glu Val Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Arg His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>506
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>8_1H7
<400>506
Met Leu Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Val Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Leu Thr
145
<210>507
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>8_1H9
<400>507
Met Leu Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Leu Glu Val Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Asp Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Arg His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Leu Thr
145
<210>508
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>GAT1_21F12
<400>508
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Asn Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Val Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>509
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>GAT1_24G3
<400>509
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Phe Val Ser Gly Tyr Tyr Glu Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro Tyr Ile Leu Met Tyr Glu Lys
130 135 140
Leu Thr
145
<210>510
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>GAT1_29G1
<400>510
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Val Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Gly
115 120 125
Val Cys Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Ala
145
<210>511
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>GAT1_32G1
<400>511
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Val Ser Gly Tyr Tyr Glu Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>512
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>GAT2_15G8
<400>512
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Lys Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Asn Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Val Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>513
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>GAT2_19H8
<400>513
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Val Ser Gly Tyr Tyr Glu Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Cys Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>514
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>GAT2_21F1
<400>514
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Ile Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Gly Gly Thr Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Arg His Ala Glu Glu
85 90 95
Leu Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Val Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Gly
115 120 125
Val Tyr Asp Ile Pro Pro Ile Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>515
<211>18
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>RBS
<222>(0)...(0)
<223>B.licheniformis ribosome binding site
<400>515
aactgaagga ggaatctc 18
<210>516
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>GY12_7G1
<400>516
atgattgagg tgaaaccgat taacgcagag gatacctatg aactaaggca taggatactc 60
agaccaaacc agccgataga ggtttgtatg tatgaaaccg atttacttcg tggtgcgttt 120
cacttaggcg gcttttacag gggcaagctg atttccatag cttcattcca ccaggccgag 180
cattcagatc tcgagggcca gaaacaatac caactccgag gtatggctac cttggaaggt 240
tatcgtgaac agaaagcggg atcgacccta attcaacacg ctgaacagat ccttcggaag 300
cggggggcgg acatgctatg gtgcaatgcg cggacaaccg ccgctggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agaggtattt gatacgccgc cagtaggacc tcacatccta 420
atgtataaac gcctcacata a 441
<210>517
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>GY12_5A11
<400>517
atgattgaag tgaaaccgat taacgcagag gatacctatg aactaaggca taggatactc 60
agaccaaacc agccgataga ggtttgtatg tatgaaagcg atttacttcg tggtgcgttt 120
cacttaggcg gcttttacag gggcaagctg atttccatag cttcattcca ccaggccgag 180
cattcagaac tccagggcca gaaacaatac caactccgag gtatggctac cttggaaggt 240
tatcgtgacc agaaagcggg atcgagccta attagacacg ctgaacagat ccttcggaag 300
cggggggcgg acatgctatg gtgcaatgcg cggacatccg cctctggcta ctacaaaaag 360
ttaggcttca gtgagcaggg agaggtattt gatacgccgc cagtaggacc tcacatccta 420
atgtataaac gcatcacata a 441
<210>518
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>GY12_4H12
<400>518
atgattgagg tgaaaccgat taacgcagag gagacctatg aactaaggca taggatactc 60
agaccacacc agccgataga ggtttgtatg tatgaaaccg atttacttcg tggtgcgttt 120
cacttaggcg gcttttacag gggcaagctg atttccatag cttcattcca ccaggccgag 180
catccagaac tccagggcca gaaacaatac caactccgag gtatggctac cttggaaggt 240
tatcgtgacc agaaagcggg atcgagccta attaaacacg ctgaacagat ccttcggaag 300
cggggggcgg acatgctatg gtgcaatgcg cggacatccg ccgctggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agaggtattt gaaacgccgc cagtaggacc tcacatcgta 420
atgtataaac gcctcacata a 441
<210>519
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>D_S00218358_B-2A3
<400>519
atgatagaag tgaaacctat taacgcagag gagacttacg aacttcgaca caagatcctg 60
cgccctaatc agccgataga ggtatgcatg tatgaaagcg atctgctgcg gggctcgttc 120
catttgggcg ggttctatcg tggccaattg atctcgattg cgagtttcca caaagctgaa 180
cacccagaac tgcaagggca aaagcagtat caattacgag ggatggcgac cctcgaagga 240
ttccgtgagc agaaggctgg ctcttcgctt attaggcacg ccgaggagat actacggaat 300
aaaggggcag atctgctttg gtgtaatgca cgcacgacag cctccggtta ctataaaagg 360
cttggttttt gtgagcacgg cgaagttttc gaaaccccgc cggttgggcc gcacattctt 420
atgtacaaaa aactcactta a 441
<210>520
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>D_S00218372_B-2B5
<400>520
atgatagaag tgaaacctat taacgcagag gagacttacg aacttcgaca caagatcctg 60
cgccctaatc agccgataga ggtatgcatg tatgaaagcg atctgctgcg gggctcgttc 120
catttgggcg ggttctatcg tggccaattg atctcgattg cgagtttcca caaagctgaa 180
cacccagaac tgcaagggca aaagcagtat caattacgag ggatggcgac cctcgaagga 240
ttccgtgagc agaaggctgg ctcttcgctt attaggcacg ccgaggagat actacggaat 300
aaaggggcag atctgctttg gtgtaatgca cgcacgacag cctccggtta ctataaaagg 360
cttggtttta gtgagcacgg cgaagttttc gaaaccccgc cggttgggcc gcacattctt 420
atgtacaaaa gaatcactta a 441
<210>521
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>D_S00218373_B-2B6
<400>521
atgatagaag tgaaacctat taacgcagag gagacttacg aacttcgaca caagatcctg 60
cgccctaatc agccgataga ggcatgcatg tatgaaagcg atctgctgcg gggctcgttc 120
catttgggcg ggttctatcg tggccaattg atctcgattg cgagtttcca caaagctgaa 180
cactcagaac tgcaagggca aaagcagtat caattacgag ggatggcgac cctcgaagga 240
ttccgtgagc agaaggctgg ctcttcgctt attaggcacg ccgaggagat actacggaat 300
aaaggggcag atctgctttg gtgtaatgca cgcacgacag cctccggtta ctataaaagg 360
cttggtttta gtgagcacgg cgaagttttc gaaaccccgc cggttgggcc gcacattctt 420
atgtacaaaa aactcactta a 441
<210>522
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>D_S00267061_B-3B8
<400>522
atgatagaag tgaaacctat taacgcagag gagacttacg aacttcgaca caagatcctg 60
cgccctaatc agccgataga ggtatgcatg tatgaaagcg atctgctgcg gggctcgttc 120
catttgggcg ggttctatcg tggccaattg atctcgattg cgagtttcca caaagctgaa 180
cactcagaac tgcaagggca aaagcagtat caattacgag ggatggcgac cctcgaagga 240
ttccgtgagc agaaggctgg ctcttcgctt attaggcatg ccgaggagat actacggaat 300
aaaggggcag atctgctttg gtgtaatgca cgcacgacag cctccggtta ctataaaagg 360
cttggtttta gtgagcacgg cgaagttttc gaaaccccgc cggttgggcc gcacattctt 420
atgtacaaaa gaatcactta a 441
<210>523
<211>440
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>D_S00218516_B-3H5
<400>523
atgatagaag tgaaacctat taacgcagag gagacttacg aacttcgaca caagatcctg 60
cgccctaatc agccgataga ggcatgcatg tatgaaagcg atctgctgcg gggctcgttc 120
catttgggcg ggttctatcg tggccaattg atctcgattg cgagtttcaa caaagctgaa 180
cactcagaac tgcaagggca aaagcagtat caattacgag ggatggcgac cctcgaagga 240
ttccgtgagc agaaggctgg ctcttcgctt attaggcacg ccgaggagat actacggaat 300
aaaggggcag atctgctttg gtgtaatgca cgcacgacag cctccggtta ctataaaagg 360
cttggtttta gtgagcacgg cgaagttttc gaaaccccgc cggttgggcc gcacattctt 420
atgtacaaaa gaatcactta 440
<210>524
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>D_S00218540_B-4B5
<400>524
atgatagaag tgaaacctat taacgcagag gagacttacg aacttcgaca caagatcctg 60
cgccctaatc agccgataga ggtatgcatg tatgaaaccg atctgctgcg gggctcgttc 120
catttgggcg ggttctatcg tggccaattg atctcgatcg cgagtttcca caaagctgaa 180
cactcagaac tgcaagggca aaagcagtat caattacgag ggatggcgac cctcgaagga 240
ttccgtgagc agaaggctgg ctcttcgctt attaggcacg ccgaggagat actacggaat 300
aaaggggcag atctgctttg gtgtaatgca cgcacgacag cctccggtta ctataaaaga 360
cttggtttta gtgagcacgg cgaagttttc gaaaccccgc cggttgggcc gcacattctt 420
atgtacaaaa aactcactta a 441
<210>525
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>D_S00218549_B-4C2
<400>525
atgatagaag tgaaacctat taacgcagag gagacttacg aacttcgaca caagatcctg 60
cgccctaatc agccgataga ggtatgcatg tatgaaagcg atctgctgcg gggcgcgttc 120
catttgggcg ggttctatcg tggccaattg atctcgattg cgagtttcca caaagctgag 180
caccctgaac tgcaagggca aaagcagtat caattacgag ggatggcgac cctcgaagga 240
ttccgtgagc agaaggctgg ctcttcgctt attaggcacg ccgaggagat actacggaat 300
agaggggcag atctgctttg gtgtaatgca cgcacgacag cctccggtta ctataaaagg 360
cttggtttta gtgagcacgg cgaagttttc gaaaccccgc cggttgggcc gcacattctt 420
atgtacaaaa gaatcactta a 441
<210>526
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>D_S00267186_B-4G1
<400>526
atgatagaag tgaaacctat taacgcagag gagacttacg aacttcgaca caagatcctg 60
cgccctaatc agccgataga ggtatgcatg tatgaaagcg atctgctgcg gggcgcgttc 120
catttgggcg ggttctatcg tggccaattg atctcgattg cgagtttcca caaagctgaa 180
cacccagaac tgcaagggca aaagcagtat caattacgag ggatggcgac cctcgaagga 240
ttccgtgagc agaaggctgg ctcttcgctt attaggcacg ccgaggagat actacggaat 300
agaggggcag atctgctttg gtgtaatgca cgcacgacag cctccggtta ctataaaagg 360
cttggtttta gtgagcacgg cgaagttttc gaaaccccgc cggttgggcc gcacattctt 420
atgtacaaaa gaatcactta a 441
<210>527
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>D_S00218594_B-4G11
<400>527
atgatagaag tgaaacctat taacgcagag gagacttacg aacttcgaca caagatcctg 60
cgccctaatc agccgataga ggtatgcatg tatgaaagcg atctgctgca gggctcgttc 120
catttgggcg ggttctatcg tggccaattg atctcgattg cgagtttcca caaagctgaa 180
cactcagaac tgcaagggca aaagcagtat caattacgag ggatggcgac cctcgaagga 240
ttccgtgagc agaaggctgg ctcttcgctt attaggcacg ccgaggagat actacggaat 300
agaggggcag atctgctttg gtgtaatgca cgcacgacag cctccggtta ctataaaagg 360
cttggtttta gtgagcacgg cgaagttttc gaaaccccgc cggttgggcc gcacattctt 420
atgtacaaaa aactcactta a 441
<210>528
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>D_S00267527_B-8G6
<400>528
atgatagaag tgaaacctat taacgcagag gagacttacg aacttcgaca caagatcctg 60
cgccctaatc agccgataga ggcatgcatg tatgaaagcg atctgctgcg ggacgcgttc 120
catttgggcg ggttctatcg tggccaattg atctcgattg cgagtttcca caaagctgaa 180
cactcagaac tgcaagggca aaagcagtat caattacgag ggatggcgac cctcgaagga 240
ttccgtgagc agaaggctgg ctcttcgctt attaggcacg ccgaggagat actacggaat 300
aaaggggcag atctgctttg gtgtaatgca cgcacgacag cctccggtta ctataagagg 360
cttggtttta gtgagcacgg cgaagttttc gaaaccccgc cggttgggcc gcacattctt 420
atgtacaaaa aactcactta a 441
<210>529
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>D_S00220826_C3G4
<400>529
atgctagagg tgaaaccgat taacgcagag gatacctatg aactaaggca taaaatactc 60
agaccaaacc agccgataga agcgtgtatg tatgaaaccg atttacttcg tggttcattt 120
cacttaggcg gcttttacag gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagaac tcgaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgagtcta attaaacatg ctgaagaaat tcttcgtaag 300
aggggggcgg acttgctttg gtgcaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agaggtattt gatacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggatcacata a 441
<210>530
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>D_S00221015_C6A1
<400>530
atgctagagg tgaaaccgat taacgcagag gatacctatg aactaaggca taaaatactc 60
agaccaaacc agccgttaga agcgtgtatg tatgaaaccg atttacttcg tggtgcattt 120
cacttaggcg gcttttacag gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagaac tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgagtcta attaaacacg ctgaagaaat tcttcgtaag 300
aggggggcgg acttgctttg gtgcaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agaggtattt gatacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggatcacata a 441
<210>531
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>D_S00221055_C6D5
<400>531
atgctagagg tgaaaccgat taacgcagag gatacctatg aactaaggca taaaatactc 60
agaccaaacc agccgataga agcgtgtatg tatgaatccg atttacttcg tggttcattt 120
cacttaggcg gcttttacag gggcaaactg atttccatag cttcattcca ccaggccgag 180
cattcagaac tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgagtcta attaaacacg ctgaagaaat tcttcgtaag 300
aagggggcgg acttgctttg gtgcaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agaggtattt gatacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggatcacata a 441
<210>532
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>D_S00221106_C7A8
<400>532
atgctagagg tgaaaccgat taacgcagag gatacctacg aactaaggca taaaatactc 60
agaccaaacc agccgataga agcgtgtatg tatgaatctg atttacttcg tggttcattt 120
cacttaggcg gcttttacag gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagaac tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgagtcta attaaacacg ctgaagaaat tcttcgtaag 300
aggggggcgg acttgctttg gtgcaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agaggtattt gatacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggatcacata a 441
<210>533
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>D_S00221021_C7B11
<400>533
atgctagagg tgaaaccgat taacgcagag gatacctatg aactaaggca taaaatactc 60
agaccaaacc agccgataga agcgtgtatg tatgaatccg atttactttg tggttcattt 120
cacttaggcg gcttttacag gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagaac tcgaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgagtcta attaaacatg ctgaagaaat tcttcgtaag 300
aagggggcgg acttgctttg gtgcaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agaggtattt gatacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggatcacata a 441
<210>534
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>D_S00221095_C8B1
<400>534
atgctagagg tgaaaccgat taacgcagag gatacctatg aactaaggca taaaatactc 60
agaccaaacc agccgttaga agcgtgtatg tatgaatctg atttacttcg tggttcattt 120
cacttaggcg gcttttacag gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagaac tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgagtcta attaaacacg ctgaagaaat tcttcgtaag 300
aagggggcgg acttgctttg gtgcaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agaggtattt gatacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggatcacata a 441
<210>535
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>D_S00221246_C8G4
<400>535
atgctagagg tgaaaccgat taacgcagag gatacctatg aactaaggca taaaatactc 60
agaccaaacc agccgataga agcgtgtatg tatgaatccg atttacttcg tggtgcattt 120
cacttaggcg gcttttacag gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagaac tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgagtcta attaaacacg ctgaagaaat tcttcgtaag 300
aggggggcgg acttgctttg gtgcaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agaggtattt gatacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggatcacata a 441
<210>536
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>D_S00221401_C10F3
<400>536
atgctagagg tgaaaccgat taacgcagag gatacctatg aactaaggca tgaaatactc 60
agaccaaacc agccgataga agcgtgtatg tatgaatctg atttacttcg tggttcattt 120
cacttaggcg gcttttacag gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagaac tcgaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgggtcta attaaacacg ctgaagaaat tcttcgtaag 300
aggggggcgg acttgctttg gtgcaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agaggtattt gatacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggatcacata a 441
<210>537
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>D_S00224699_17-9C8
<400>537
atgatagagg tgaaaccgat taacgcagag gatacctatg aactaaggca tagaatactc 60
agaccaaacc agccgataga agcgtgtatg tatgaaagcg atttacttcg tggtgcattt 120
cacttaggcg gcttttacgg gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagaac tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgagtcta gttaaacacg ctgaagaaat tcttcgtaag 300
aggggggcgg acttgctttg gtgcaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agagatattt gaaacgccgc cggtaggacc tcacatcctg 420
atgtataaaa ggatcacata a 441
<210>538
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>D_S00224736_17-9G9
<400>538
atgatagagg tgaaaccgat taacgcagag gatacctatg aactaaggca tagaatactc 60
agaccaaacc agccgataga agcgtgtatg tatgaaagcg atttacttcg tggtgcattt 120
cacttaggcg gctattacag gggcaaactg atttccatag cgtcattcca ccaggccgag 180
cactcagacc tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgagtcta attaaacacg ctgaagaaat tcttcgtaag 300
aggggggcgg acatgctttg gtgcaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agaggtattt gacacgccgc cggtaggacc tcacatcctg 420
atgtataaaa ggctcacata a 441
<210>539
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>D_S00224759_17-10A8
<400>539
atgatagagg tgaaaccgat taacgcagag gatacctatg aactaaggca taaaatactc 60
agaccaaacc agccgataga agcgtgtatg tatgaaagcg atttacttcg tggtgcattt 120
cacttaggcg gctattacag gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagaac tccaaggcca gaaacagtac cagctccggg gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgagtcta gttaaacacg ctgaagaaat tcttcgtaag 300
aggggggcgg acttgctttg gtgcaatgcg cggacgtccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agagatattt gaaacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggctcacata a 441
<210>540
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>D_S00224766_17-10B3
<400>540
atgatagagg tgaaaccgat taacgcagag gatacctatg aactaaggca tagaatactc 60
agaccaaacc agccgataga agcgtgtatg tttgaaagcg atttacttcg tggtgcattt 120
cacttaggcg gcttttacgg gggcaaactg atttccatag cttcattcca ccaggctgag 180
cactcagaac tccaaggcca gaaacagtac cagctccgag gtgtggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgagtcta attaaacacg ctgaagaaat tcttcgtaag 300
aggggggcgg acatgctttg gtgtaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agaggtattt gatacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggatcacata a 441
<210>541
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>D_S00224783_17-10C8
<400>541
atgatagagg tgaaaccgat taacgcagag gatacctatg aactaaggca tagaatactc 60
agaccaaacc agccgataga agcgtgtatg tttgaaagcg atttacttcg tggtgcattt 120
cacttaggcg gcttttacgg gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagaac tccaaggcca gaaacagtac cagctccgag gtatggctac cttggagggt 240
tatcgtgagc agaaagcggg atcgagtcta attaaacacg ctgaagaaat tcttcgtaag 300
aggggggcgg acttgctttg gtgtaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttcg gcgagcaggg agagatattt gatacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggctcacata a 441
<210>542
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>D_S00260479_17-12A10
<400>542
atgatagagg tgaaaccgat taacgcagag gatacctatg aactaaggca tagaatactc 60
agaccaaacc agccgataga agcgtgtatg tttgaaagcg atttacttcg tggtgcattt 120
cacttaggcg gcttttacgg gggcaaactg atttccatag cgtcattcca ccaggccgag 180
cactcagaac tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgatc agaaagcggg atcgagtcta attagacacg ctgaacaaat tcttcgtaag 300
aggggggcgg acatgctttg gtgcaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agaggtattt gaaacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggctcacata a 441
<210>543
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>D_S00260491_17-12B10
<400>543
atgatagagg tgaaaccgat taacgcagag gatacctatg aactaaggca tagaatactc 60
agaccaaacc agccgataga agcgtgtatg tttgaaagcg atttacttcg tggtgcgttt 120
cacttaggcg gctattacgg gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagaac tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgagtcta attaaacacg ctgaagaaat tcttcgtaag 300
aggggggcgg acttgctttg gtgtaatgcg cggacgtccg cctcaggcta ctacaaaaag 360
ctaggcttca gcgagcaggg agagatattt gaaacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggatcacata a 441
<210>544
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>D_S00260546_17-12H5
<400>544
atgatagagg tgaaaccgat taacgcagag gatacctatg aactaaggca tagaatactc 60
agaccaaacc agccgataga agcgtgtatg tttgaaagcg atttacttcg tggtgcattt 120
cacttaggcg gcttttacgg gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagaac tccaaggcca gaaacagtac cagctccgag gtatggctac cttggagggt 240
tatcgtgagc agaaagcggg atcgagtcta attagacacg ctgaagaaat tcttcgtaag 300
aggggggcgg acttgctttg gtgtaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agagatattt gaaacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggatcacata a 441
<210>545
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>D_S00260588_17-13C11
<400>545
atgctagaag tgaaaccgat taacgcagag gatacctatg aactaaggca tagaatactc 60
agaccaaacc agccgataga agcgtgtatg tttgaaagcg atttacttcg tggtgcattt 120
cacttaggcg gctattacgg gggcaaactg atttccatag cttcattcca ccgggccgag 180
cactcagaac tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgagtcta gttaaacacg ctgaagaaat tcttcgtaag 300
aggggggcgg acttgctttg gtgtaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agagatattt gacacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggatcacata a 441
<210>546
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>D_S00260694_17-14F9
<400>546
atgatagaag tgaaaccgat taacgcagag gatacctatg aactaaggca taaaatactc 60
agaccaaacc agccgataga agcgtgtatg tttgaaagcg atttacttcg tggtgcattt 120
cacttaggcg gctattacgg gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcggaac tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgagtcta attaaacacg ctgaagaaat tcttcgtaag 300
aggggggcgg acttgctttg gtgtaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agagatattt gaaacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggctcacata a 441
<210>547
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>D_S00260743_17-15B10
<400>547
atgatagaag tgaaaccgat taacgcagag gatacctatg aactaaggca taaaatactc 60
agaccaaacc agccgataga agcgtgtatg tttgaaagcg atttacttcg tggtgcattt 120
cacttaggcg gcttttacgg gggcaaactg atttccatag cttcattcca ccaggccgag 180
cacttagacc tcgaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgagtcta attaaacacg ctgaagaaat tcttcgtaag 300
aggggggcgg acttgctttg gtgcaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agagatattt gaaacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggctcacata a 441
<210>548
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>D_S00260793_17-15G12
<400>548
atgatagaag tgaaaccgat taacgcagag gatacctatg aactaaggca tagaatactc 60
agaccaaacc agccgataga agcgtgtatg tttgaaagcg atttacttcg tggtgcattt 120
cacttgggcg gctattacag gggcaaactg atttccgtag cgtcattcca ccaggccgag 180
cactcagaac tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgatc agaaagcggg atcgagtcta gttaaacacg ctgaagaaat tcttcgtaag 300
aggggggcgg acttgctttg gtgtaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agagatattt gaaacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggctcacata a 441
<210>549
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>D_S00260796_17-15H3
<400>549
atgatagagg tgaaaccgat taacgcagag gatacctatg aactaaggca tagaatactc 60
agaccaaacc agccgataga agcgtgtatg tttgaaagcg atttacttcg tggtgcattt 120
cacttaggcg gctattacgg gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagaac tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaggcggg atcgagtcta attaaacacg ctgaagaaat tcttcgtaag 300
aggggggcgg acttgctttg gtgtaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agaggtattc gacacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggatcacata a 441
<210>550
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>17-16A10
<400>550
atgatagagg tgaaaccgat taacgcagag gatacctatg aactaaggca tagaatactc 60
agaccaaacc agccgataga agcgtgtatg tttgaaagcg atttacttcg tggtgcattt 120
cacttaggcg gctattacag gggcaaactg atttccatag cgtcattcca ccaggccgag 180
cactcagaac tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgagtcta gttaaacacg ctgaagaaat tcttcgtaag 300
aggggggcgg acatgctttg gtgtaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agagatattt gatacgccgc cagtaggacc tcacatcctg 420
atgtataaaa gga tcacatg a 441
<210>551
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>D_S00260847_17-16D6
<400>551
atgatagaag tgaaaccgat taacgcagag gatacctatg aactaaggca tagaatactc 60
agaccaaacc agccgataga agcgtgtatg tttgaaagcg atttacttcg tggtgcattt 120
cacttgggcg gctattacag gggcaaactg atttccgtag cgtcattcca ccaggccgag 180
cactcagaac tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgatc agaaagcggg atcgagtcta gttaaacacg ctgaagaaattcttcgt aag 300
aggggggcgg acttgctttg gtgtaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agagatattt gaaacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggctcacata a 441
<210>552
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>D_S00260855_17-16F2
<400>552
atgatagaag tgaaaccgat taacgcagag gatacctatg aactaaggca taaaatactc 60
agaccaaacc agccgataga agcgtgtatg tttgaaagcg atttacttcg tggtgcattt 120
cacttgggcg gcttttacag gggcaaactg atttccatag cgtcattcca ccaggccgag 180
cactcagacc tcgaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgagtcta attaaacacg ctgaagaaat tcttcgtaag 300
aggggggcgg acatgctttg gtgtaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg ggagatattt gaaacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggatcacata a 441
<210>553
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>D_S00260862_17-16F9
<400>553
atgatagagg tgaaaccgat taacgcagag gatacctatg aactaaggca tagaatactc 60
agaccaaacc agccgataga agcgtgtatg tttgaaagcg atttacttcg tggtgcattt 120
cacttaggcg gctattacag gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagaac tccaaggcca ggaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgatc agaaagcggg atcgagtcta gttaaacacg ctgaagaaat tcttcgtaag 300
aggggggcgg acttgctttg gtgcaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agaggtattt gaaacgccgc cagtaggacc tcacatcctg 420
atgta taaaa ggatcacata a 441
<210>554
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>D_S00260904_17-17B3
<400>554
atgatagaag tgaaaccgat taacgcagag gatacctatg aactaaggca taaaatactc 60
agaccaaacc agccgataga agcgtgtatg tttgaaagcg atttacttcg tggtgcactt 120
cacttaggcg gctattacag gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagacc tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgagtctg gttaaacacg ctgaagaaat tcttcgtaag 300
aggggggcgg acttgctttg gtgtaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agagatattt gaaacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggatcacata a 441
<210>555
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>D_S00260929_17-17D4
<400>555
atgatagagg tgaaaccgat taacgcagag gatacctatg aactaaggca tagaatactc 60
agaccaaacc agccgataga agcgtgtatg tttgaaagcg atttacttcg tggtgcattt 120
cacttaggcg gctattacag gggcaaactg atttccatag cgtcattcca ccaggccgag 180
cactcagaac tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgagtcta gttaaacacg ctgaagaaat tctccgtaag 300
aggggggcgg acttgctttg gtgtaatgcg cggacgtccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agaggtattt gatacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggatcacata a 441
<210>556
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>D_S00260940_17-17F3
<400>556
atgatagaag tgaaaccgat taacgcagag gatacctatg aactaaggca tagaatactc 60
agaccaaacc agccgataga agcgtgtatg tttgaaagcg atttacttcg tggtgcattt 120
cacttaggcg gctattacag gggcaaactg atttccatag cttcattcca ccaggccgag 180
cgctcagacc tccaaggcca gaaacagtac cagctccgag gtatggctac cttggagggt 240
tatcgtgatc agaaagcggg atcgagtcta attagacacg ctgaagaaat tcttcgtaag 300
aggggggcgg acttgctttg gtgtaatgcg cggacgtccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agagatattt gaaacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggatcacata a 441
<210>557
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>D_S00260942_17-17F5
<400>557
atgatagagg tgaaaccgat taacgcagag gatacctatg aactaaggca tagaatactc 60
agaccaaacc agccgataga agcgtgtatg tttgaaagcg atttacttcg tggtgcactt 120
cacttaggcg gcttttacag gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagaac tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgagtcta gttaaacacg ctgaagaaat tcttcgtaag 300
aggggggcgg acttgctttg gtgtaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agagatattt gaaacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggatcacata a 441
<210>558
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>D_S00260945_17-17F8
<400>558
atgatagaag tgaaaccgat taacgcagag gatacctatg aactaaggca tagaatactc 60
agaccaaacc agccgataga agcgtgtatg tttgaaagcg atttacttcg tggtgcattt 120
cacttaggcg gctattacag gggcaaactg atttccatag cttcattcca tcaggccgag 180
cactcagaac tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgagtcta gttaaacacg ctgaagaaat tcttcgtaag 300
aggggggcgg acatgctttg gtgtaatgcg cggacgtccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agaggtattt gacacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggatcacata a 441
<210>559
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>D_S00224368_18-8A7
<400>559
atgatagaag tgaaaccgat taacgcagag gatacctatg aactaaggca tagaatactc 60
agaccaaacc agccgataga agcgtgtatg tttgaaagcg atttacttcg tggtgcactt 120
cacttaggcg gcttttacgg gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagacc tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgagtcta attaaacacg ctgaagaaat tcttcgtaag 300
aggggggcgg acttgctttg gtgtaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agaggtattt gatacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggatcacata a 441
<210>560
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>D_S00224350_18-8B7
<400>560
atgatagagg tgaaaccgat taacgcagag gatacctatg aactaaggca tagaatactc 60
agaccaaacc agccgataga agcgtgtatg tttgaaagcg atttacttcg tggtgcattt 120
cacttaggcg gctattacag gggcaaactg atttccatag cgtcattcca ccaggccgag 180
cactcagacc tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgagtcta attagacacg ctgaagaaat tcttcgtaag 300
aggggggcgg acatgctttg gtgcaacgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agagatattt gatacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggatcacata a 441
<210>561
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>D_S00224416_18-9A1
<400>561
atgatagaag tgaaaccgat taacgcagag gatacctatg aactaaggca tagaatactc 60
agaccaaacc agccgataga agcgtgtatg tttgaaagcg atttacttcg tggtgcactt 120
cacttaggcg gctattacag gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagacc tcgaaggccg gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgagtcta attaaacacg ctgaagaaat tcttcgtaag 300
aggggggcgg acttgctttg gtgtaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agaggtattt gacacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggatcacata a 441
<210>562
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>D_S00224422_18-9A7
<400>562
atgatagaag tgaaaccgat taacgcagag gatacctatg aactaaggca tagaatactc 60
agaccaaacc agccgataga agcgtgtatg tatgaaagcg atttacttcg tggtgcattt 120
cacttaggcg gctattacag aggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcggaac tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgagtcta attaaacacg ctgaagaaat tcttcgtaag 300
aggggggcgg acatgctttg gtgtaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agaggtattt gatacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggatcacata a 441
<210>563
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>D_S00224466_18-9F3
<400>563
atgatagagg tgaaaccgat taacgcagag gatacctatg aactaaggca tagaatactc 60
agaccaaacc agccgataga agcgtgtatg tatgaaagcg atttacttcg tggtgcattt 120
cacttaggcg gcttttacag gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagaac tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgagtcta gttaaacacg ctgaagaaat tcttcgtaag 300
aggggggcgg acatgctttg gtgtaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggtttca gcgagcaggg agaggtattt gacacgccgc cagtaggacc tcacatcctg 420
atgt ataaaa ggatcacata a 441
<210>564
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>D_S00261096_18-24D3
<400>564
atgatagagg tgaaaccgat taacgcagag gatacctatg aactaaggca taaaatactc 60
agaccaaacc agccgatgga agcgtgtatg tatgaaagcg atttacttcg tggtgcattt 120
cacttaggcg gcttttacag gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagacc tcgaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgagtcta attaaacacg ctgaagaaat tcttcgtaag 300
aggggggcgg acttgctttg gtgtaatgcg cggacgtccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agagatattt gatacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggatcacata a 441
<210>565
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>D_S00261192_18-25F3
<400>565
atgatagaag tgaaaccgat taacgcagag gatacctatg aactaaggca taaaatactc 60
agaccaaacc agccgataga agcgtgtatg tttgaaagcg atttacttcg tggtgcattt 120
cacttaggcg gctattacgg gggcaaactg atttccatag cgtcattcca ccaggccgag 180
cactcggaac tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgagtcta attagacacg ctgaagaaat tctccgtaag 300
aggggggcgg acatgctttg gtgtaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agagatattt gaaacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggatcacata a 441
<210>566
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>D_S00261438_18-28D9
<400>566
atgatagarg tgaaaccgat taacgcagag gatacctatg aactaaggca tagaatactc 60
agaccaaacc agccgataga agcgtgtatg tttgaaagcg atttacttcg tggtgcattt 120
cacttaggcg gcttttacag gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcggaac tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcaactcta gttaaacacg ctgaagaaat ccttcgtaag 300
aggggggcgg acatgctttg gtgtaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agagatattt gacacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggatcacata a 441
<210>567
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>D_S00261440_18-28D11
<400>567
atgatagaag tgaaaccgat taacgcagag gatacctatg aactaaggca tagaatactc 60
agaccaaacc agccgataga agcgtgtatg tttgaaagcg atttacttcg tggtgcattt 120
cacttaggcg gcttttacag gggcaaactg atttccatag cttcattcca ccaggccgag 180
cactcagacc tccaaggcca gaaacagtac cagctccgag gtatggctac cttggaaggt 240
tatcgtgagc agaaagcggg atcgagtcta attagacacg ctgaagaaat tcttcgtaag 300
aggggggcgg acttgctttg gtgtaatgcg cggacatccg cctcaggcta ctacaaaaag 360
ttaggcttca gcgagcaggg agagatattt gaaacgccgc cagtaggacc tcacatcctg 420
atgtataaaa ggatcacata a 441
<210>568
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>GY12_7G1
<400>568
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Val Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Asp Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Ile Gln His Ala Glu Gln
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Thr Ala Ala Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Leu Thr
145
<210>569
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>GY12_5A11
<400>569
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Val Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Asp Gln Lys Ala Gly Ser Ser Leu Ile Arg His Ala Glu Gln
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>570
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>GY12_4H12
<400>570
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Glu Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro His Gln Pro Ile Glu Val Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Asp Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Gln
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ala Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Glu Thr Pro Pro Val Gly Pro His Ile Val Met Tyr Lys Arg
130 135 140
Leu Thr
145
<210>571
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>D_S00218358_B-2A3
<400>571
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Glu Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Ile Glu Val Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ser Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Gln Leu Ile Ser Ile Ala Ser Phe His Lys Ala Glu His Pro Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Phe Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Arg His Ala Glu Glu
85 90 95
Ile Leu Arg Asn Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Thr Ala Ser Gly Tyr Tyr Lys Arg Leu Gly Phe Cys Glu His Gly Glu
115 120 125
Val Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>572
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>D_S00218372_B-2B5
<400>572
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Glu Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Ile Glu Val Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ser Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Gln Leu Ile Ser Ile Ala Ser Phe His Lys Ala Glu His Pro Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Phe Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Arg His Ala Glu Glu
85 90 95
Ile Leu Arg Asn Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Thr Ala Ser Gly Tyr Tyr Lys Arg Leu Gly Phe Ser Glu His Gly Glu
115 120 125
Val Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>573
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>D_S00218373_B-2B6
<400>573
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Glu Tvr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ser Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Gln Leu Ile Ser Ile Ala Ser Phe His Lys Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Phe Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Arg His Ala Glu Glu
85 90 95
Ile Leu Arg Asn Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Thr Ala Ser Gly Tyr Tyr Lys Arg Leu Gly Phe Ser Glu His Gly Glu
115 120 125
Val Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>574
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>D_S00267061_B-3B8
<400>574
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Glu Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Ile Glu Val Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ser Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Gln Leu Ile Ser Ile Ala Ser Phe His Lys Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Phe Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Arg His Ala Glu Glu
85 90 95
Ile Leu Arg Asn Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Thr Ala Ser Gly Tyr Tyr Lys Arg Leu Gly Phe Ser Glu His Gly Glu
115 120 125
Val Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>575
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>D_S00218516_B-3H5
<400>575
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Glu Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ser Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Gln Leu Ile Ser Ile Ala Ser Phe Asn Lys Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Phe Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Arg His Ala Glu Glu
85 90 95
Ile Leu Arg Asn Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Thr Ala Ser Gly Tyr Tyr Lys Arg Leu Gly Phe Ser Glu His Gly Glu
115 120 125
Val Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>576
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>D_S00218540_B-4B5
<400>576
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Glu Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Ile Glu Val Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ser Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Gln Leu Ile Ser Ile Ala Ser Phe His Lys Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Phe Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Arg His Ala Glu Glu
85 90 95
Ile Leu Arg Asn Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Thr Ala Ser Gly Tyr Tyr Lys Arg Leu Gly Phe Ser Glu His Gly Glu
115 120 125
Val Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>577
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>D_S00218549_B-4C2
<400>577
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Glu Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Ile Glu Val Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Gln Leu Ile Ser Ile Ala Ser Phe His Lys Ala Glu His Pro Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Phe Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Arg His Ala Glu Glu
85 90 95
Ile Leu Arg Asn Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Thr Ala Ser Gly Tyr Tyr Lys Arg Leu Gly Phe Ser Glu His Gly Glu
115 120 125
Val Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu MetTyr Lys Arg
130 135 140
Ile Thr
145
<210>578
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>D_S00267186_B-4G1
<400>578
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Glu Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Ile Glu Val Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Gln Leu Ile Ser Ile Ala Ser Phe His Lys Ala Glu His Pro Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Phe Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Arg His Ala Glu Glu
85 90 95
Ile Leu Arg Asn Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Thr Ala Ser Gly Tyr Tyr Lys Arg Leu Gly Phe Ser Glu His Gly Glu
115 120 125
Val Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>579
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>D_S00218594_B-4G11
<400>579
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Glu Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Ile Glu Val Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Gln Gly Ser Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Gln Leu Ile Ser Ile Ala Ser Phe His Lys Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Phe Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Arg His Ala Glu Glu
85 90 95
Ile Leu Arg Asn Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Thr Ala Ser Gly Tyr Tyr Lys Arg Leu Gly Phe Ser Glu His Gly Glu
115 120 125
Val Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>580
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>D_S00267527_B-8G6
<400>580
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Glu Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Arg Asp Ala Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Gln Leu Ile Ser Ile Ala Ser Phe His Lys Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Phe Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Arg His Ala Glu Glu
85 90 95
Ile Leu Arg Asn Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Thr Ala Ser Gly Tyr Tyr Lys Arg Leu Gly Phe Ser Glu His Gly Glu
115 120 125
Val Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Lys
130 135 140
Leu Thr
145
<210>581
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>D_S00220826_C3G4
<400>581
Met Leu Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ser Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>582
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>D_S00221015_C6A1
<400>582
Met Leu Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>583
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>D_S00221055_C6D5
<400>583
Met Leu Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ser Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>584
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>D_S00221106_C7A8
<400>584
Met Leu Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ser Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>585
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>D_S00221021_C7B11
<400>585
Met Leu Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Cys Gly Ser Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>586
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>D_S00221095_C8B1
<400>586
Met Leu Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Leu Glu Ala Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ser Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Lys Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>587
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>D_S00221246_C8G4
<400>587
Met Leu Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>588
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>D_S00221401_C10F3
<400>588
Met Leu Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Glu Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ser Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Gly Leu Ile Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>589
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>D_S00224699_17-9C8
<400>589
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>590
<211>143
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>D_S00224736_17-9G9
<400>590
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Asp Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys ASn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys
130 135 140
<210>591
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>D_S00224759_17-10A8
<400>591
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Leu Thr
145
<210>592
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>D_S00224766_17-10B3
<400>592
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>593
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>D_S00224783_17-10C8
<400>593
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Gly Glu Gln Gly Glu
115 120 125
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Leu Thr
145
<210>594
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>D_S00260479_17-12A10
<400>594
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Asp Gln Lys Ala Gly Ser Ser Leu Ile Arg His Ala Glu Gln
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Leu Thr
145
<210>595
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>D_S00260491_17-12B10
<400>595
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>596
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>D_S00260546_17-12H5
<400>596
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Arg His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>597
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>D_S00260588_17-13C11
<400>597
Met Leu Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Arg Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>598
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>D_S00260694_17
<400>598
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Leu Thr
145
<210>599
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>D_S00260743_17-15B10
<400>599
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Leu Asp Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Leu Thr
145
<210>600
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>D_S00260793_17-15G12
<400>600
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Asp Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Leu Thr
145
<210>601
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>D_S00260796_17-15H3
<400>601
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>602
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>17-16A10
<400>602
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>603
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>D_S00260847_17-16D6
<400>603
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Asp Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Leu Thr
145
<210>604
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>D_S00260855_17-16F2
<400>604
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Asp Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>605
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>D_S00260862_17-16F9
<400>605
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Glu Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Asp Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>606
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>D_S00260904_17-17B3
<400>606
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Leu His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Asp Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>607
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>D_S00260929_17-17D4
<400>607
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>608
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>D_S00260940_17-17F3
<400>608
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu Arg Ser Asp Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Asp Gln Lys Ala Gly Ser Ser Leu Ile Arg His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met TyrLys Arg
130 135 140
Ile Thr
145
<210>609
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>D_S00260942_17-17F5
<400>609
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Leu His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>610
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>D_S00260945_17-17F8
<400>610
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>611
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>D_S00224368_18-8A7
<400>611
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Leu His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Asp Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>612
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>D_S00224350_18-8B7
<400>612
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Asp Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Arg His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>613
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>D_S00224416_18-9A1
<400>613
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Leu His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Asp Leu
50 55 60
Glu Gly Arg Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>614
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>D_S00224422_18-9A7
<400>614
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>615
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>D_S00224466_18-9F3
<400>615
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>616
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>D_S00261096_18-24D3
<400>616
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Met Glu Ala Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Asp Leu
50 55 60
Glu Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>617
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>D_S00261192_18-25F3
<400>617
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Arg His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>618
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>D_S00261438_18-28D9
<400>618
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>619
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>D_S00261440_18-28D11
<400>619
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Asp Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Arg His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>620
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>D_GE002256_GAT20-12F7
<220>
<221>CDS
<222>(1)...(441)
<400>620
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta atg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag att cga ggt gtg gct acc tta gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Ile Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac ctg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>621
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_GE002256_GAT20-12F7
<220>
<223>合成的蛋白质序列
<400>621
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Ile Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>622
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<223>D_GE003194_GAT20-12F7b
<220>
<221>CDS
<222>(1)...(441)
<400>622
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta atg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag att cga ggt gtg gct acc tta gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Ile Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac ctg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>623
<211>146
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<220>
<223>D_GE003194_GAT20-12F7b
<400>623
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Ile Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>624
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_GE002257_GAT20-13A2
<400>624
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga atc ctc aga cca aac cag ccg ata gaa gcg tgt atc ttt gaa 96
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Ile Phe Glu
20 25 30
agc gat tta atg cgt ggt gca ttt cac tta ggc ggc tac tac ggg ggc 144
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
aaa ctg att tcc atc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac ata ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Ile Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aaa aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>625
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_GE002257_GAT20-13A2
<220>
<223>合成的蛋白质序列
<400>625
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Ile Phe Glu
20 25 30
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Ile Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>626
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_GE002258_GAT20-13H9
<400>626
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta ctg cgt ggt gca ttt cac tta ggc ggc tac tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac acg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
caa ggc cag aaa cag tac cag att cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Ile Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac cta ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc ttc tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Phe Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
ata ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa tgg 432
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Trp
130 135 140
atc aca taa 441
Ile Thr *
145
<210>627
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_GE002258_GAT20-13H9
<220>
<223>合成的蛋白质序列
<400>627
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Ile Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Phe Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Trp
130 135 140
Ile Thr
145
<210>628
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_GE002259_GAT20-14A6
<400>628
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta atg cgt ggt gca ttt cac tta ggc ggc tac tac ggg ggc 144
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
cca ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Pro Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac ata ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Ile Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc ttc tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Phe Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>629
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_GE002259_GAT20-14A6
<220>
<223>合成的蛋白质序列
<400>629
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Pro Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Ile Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Phe Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>630
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_GE002260_GAT20-14A11
<400>630
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atc ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Ile Phe Glu
20 25 30
agc gat tta ctg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aga ctg att tcc atc gct tca ttc cac cag gcc gag cac tcg gaa att 192
Arg Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Ile
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac atg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>631
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_GE002260_GAT20-14A11
<220>
<223>合成的蛋白质序列
<400>631
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Ile Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Arg Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Ile
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>632
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_GE003195_GAT20-14A11b
<400>632
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Ash Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atc ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Ile Phe Glu
20 25 30
agc gat tta ctg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aga ctg att tcc atc gct tca ttc cac cag gcc gag cac tcg gaa att 192
Arg Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Ile
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac atg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>633
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_GE003195_GAT20-14A11b
<220>
<223>合成的蛋白质序列
<400>633
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Ile Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Arg Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Ile
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>634
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_GE002261_GAT20-14G7
<400>634
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
agc gat tta ctg cgt ggt gca ttt cac tta ggc ggc tac tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
aga ctg att tcc atc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Arg Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac ata ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Ile Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aaa aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
ata ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>635
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_GE002261_GAT20-14G7
<220>
<223>合成的蛋白质序列
<400>635
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
Arg Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Ile Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>636
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_GE002262_GAT20-14H3
<400>636
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta ttg cgt ggt gca ttt cac tta ggc ggc tac tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
aga ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Arg Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att ata cgt aag agg ggg gcg gac cta ctt tgg tgt aat gcg cgg aca 336
Ile Ile Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc ttc tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Phe Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>637
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_GE002262_GAT20-14H3
<220>
<223>合成的蛋白质序列
<400>637
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
Arg Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Ile Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Phe Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>638
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_GE002263_GAT20-16A3
<400>638
atg ata gag gta aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
agc gat tta ctg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aga ctg att tcc gtc gct tca ttc cac cag gcc gag cac acg gaa ctt 192
Arg Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac ctg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>639
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_GE002263_GAT20-16A3
<220>
<223>合成的蛋白质序列
<400>639
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Arg Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>640
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_GE003196_GAT20-16A3b
<400>640
atg ata gag gta aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
agc gat tta ctg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aga ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Arg Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac ctg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>641
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_GE003196_GAT20-16A3b
<220>
<223>合成的蛋白质序列
<400>641
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Arg Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>642
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_GE002264_GAT20-16C7
<400>642
atg ata gag gta aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atc ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Ile Phe Glu
20 25 30
agc gat tta atg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc atc gct tca ttc cac cag gcc gag cac acg gaa ctt 192
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta ctt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Leu Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac ctg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc ttc tac aaa aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Phe Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>643
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_GE002264_GAT20-16C7
<220>
<223>合成的蛋白质序列
<400>643
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Ile Phe Glu
20 25 30
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Leu Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Phe Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>644
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_GE002265_GAT20-16G8
<400>644
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta atg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta ctt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Leu Lys His Ala Glu Glu
85 90 95
att ata cgt aag agg ggg gcg gac atg ctt tgg tgt aat gcg cgg aca 336
Ile Ile Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc ttc tac aaa aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Phe Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>645
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_GE002265_GAT20-16G8
<220>
<223>合成的蛋白质序列
<400>645
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Leu Lys His Ala Glu Glu
85 90 95
Ile Ile Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Phe Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>646
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_GE002266_GAT20-17A10
<400>646
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
agc gat tta atg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aga ctg att tcc atc gct tca ttc cac cag gcc gag cac acg gaa ctt 192
Arg Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac cta ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
ata ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>647
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_GE002266_GAT20-17A10
<220>
<223>合成的蛋白质序列
<400>647
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Arg Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>648
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_GE003197_GAT20-17A10b
<400>648
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
agc gat tta atg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aga ctg att tcc atc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Arg Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac cta ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
ata ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>649
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_GE003197_GAT20-17A10b
<220>
<223>合成的蛋白质序列
<400>649
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Arg Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>650
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_GE002267_GAT20-17B11
<400>650
atg ata gag ata aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Ile Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atc ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Ile Phe Asp
20 25 30
agc gat tta atg cgt ggt gca ttt cac tta ggc ggc tac tac ggg ggc 144
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
aaa ctg att tcc atc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac ata ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Ile Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>651
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_GE002267_GAT20-17B11
<220>
<223>合成的蛋白质序列
<400>651
Met Ile Glu Ile Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Ile Phe Asp
20 25 30
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Ile Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>652
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_GE002268_GAT20-17C1
<400>652
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
agc gat tta atg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aga ctg att tcc atc gct tca ttc cac cag gcc gag cac acg gaa ctt 192
Arg Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac cta ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
ata ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>653
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_GE002268_GAT20-17C1
<220>
<223>合成的蛋白质序列
<400>653
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Arg Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>654
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_GE002269_GAT20-18D7
<400>654
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
agc gat tta ctg cgt ggt gca ttt cac tta ggc ggc tac tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
aga ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Arg Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac ata ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Ile Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gat 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Asp
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>655
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_GE002269_GAT20-18D7
<220>
<223>合成的蛋白质序列
<400>655
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
Arg Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Ile Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Asp
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>656
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_GE002270_GAT20-18D9
<400>656
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atc ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Ile Phe Glu
20 25 30
agc gat tta atg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aga ctg att tcc atc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Arg Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gtg gac cta ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Val Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>657
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_GE002270_GAT20-18D9
<220>
<223>合成的蛋白质序列
<400>657
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Ile Phe Glu
20 25 30
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Arg Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Val Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>658
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_GE002271_GAT20-20H10
<400>658
atg ata gag gta aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
agc gat tta ctg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aga ctg att tcc gtc gct tca ttc cac cag gcc gag cac acg gaa ctt 192
Arg Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac ctg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>659
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_GE002271_GAT20-20H10
<220>
<223>合成的蛋白质序列
<400>659
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Arg Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>660
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_GE002272_GAT20-22G5
<400>660
atg ata gag gta aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atc ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Ile Phe Glu
20 25 30
agc gat tta atg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aga ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Arg Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac cta ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aaa aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>661
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_GE002272_GAT20-22G5
<220>
<223>合成的蛋白质序列
<400>661
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Ile Phe Glu
20 25 30
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Arg Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>662
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_GE002273_GAT20-22H1
<400>662
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
agc gat tta ctg cgt ggt gca ttt cac tta ggc ggc tac tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
aga ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Arg Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac ata ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Ile Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gat 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Asp
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>663
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_GE002273_GAT20-22H1
<220>
<223>合成的蛋白质序列
<400>663
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Try Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
Arg Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Ile Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Asp
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>664
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_GE002274_GAT19-11C8
<400>664
atg ata gaa gtg aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga ata ctc aga cca aac cag ccg gta gaa gcg tgt atg ttt gaa 96
His Arg Ile Leu Arg Pro Asn Gln Pro Val Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta ctt cgt ggt gca ttt cac tta ggc ggc tat tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
aaa ctg gtt tcc ata gct tca ttc cac cag gcc gag cac tcg gaa ctc 192
Lys Leu Val Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctc cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
cac cgt gag cag aaa gcg gga tcg agt cta att aaa cac gct gaa gaa 288
His Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
atc ctt cgt aag agg ggg gcg gac atg ctt tgg tgc aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aaa aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>665
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_GE002274_GAT19-11C8
<220>
<223>合成的蛋白质序列
<400>665
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Val Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
Lys Leu Val Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
His Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>666
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_GE002275_GAT19-11D6
<400>666
atg ata gag gtg aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga ata ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta ctt cgt ggt gca ttt cac tta ggc ggc tat tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
aaa ctg att tcc ata gct tca ttc cac cag gcc gag cac tca gaa ctc 192
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag gaa cag tac cag ctc cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Glu Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aaa gcg gga tca act cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Val Lys His Ala Glu Glu
85 90 95
att ctt cgt aag agg ggg gcg gac atg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aaa aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
ata ttt gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>667
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_GE002275_GAT19-11D6
<220>
<223>合成的蛋白质序列
<400>667
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Glu Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>668
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_GE002276_GAT19-11D8
<400>668
atg ata gag gtg aaa ccg att aac gca gag gat acc tat gag cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp ThrTyr Gl u Leu Arg
1 5 10 15
cat aga ata ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta ctt cgt ggt gca ttt cac tta ggc ggc ttt tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tct ata gct tca ttc cac cag gcc gag cac tca gac ctc 192
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Asp Leu
50 55 60
caa ggc cag aaa cag tac cag ctc cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aaa gcg gga tca act cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Val Lys His Ala Glu Glu
85 90 95
atc ctt cgt aag agg ggg gcg gac atg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aaa aag tta ggc ttc agc gag cag gga gag 384
Ser A1a Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttt gaa acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
ctc aca taa 441
Leu Thr *
145
<210>669
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_GE002276_GAT19-11D8
<220>
<223>合成的蛋白质序列
<400>669
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Asp Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Leu Thr
145
<210>670
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_GE002277_GAT19-11H12
<400>670
atg ata gaa gtg aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aaa ata ctc aga cca aac cag ccg tta gaa gtg tgt atg tat gaa 96
His Lys Ile Leu Arg Pro Asn Gln Pro Leu Glu Val Cys Met Tyr Glu
20 25 30
agc gat tta ctt cgt ggt gca ttt cac tta ggc ggc tat tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
aaa ctg att tcc ata gct tca ttc cac cag gct gag cac cca gaa ctc 192
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctc cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gat cag aaa gcg gga tcg agt cta att aga cac gct gaa gaa 288
Tyr Arg Asp Gln Lys Ala Gly Ser Ser Leu Ile Arg His Ala Glu Glu
85 90 95
att ctt cgt aag agg ggg gcg gac atg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aaa aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>671
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_GE002277_GAT19-11H12
<220>
<223>合成的蛋白质序列
<400>671
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Leu Glu Val Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Pro Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Asp Gln Lys Ala Gly Ser Ser Leu Ile Arg His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>672
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_GE002278_GAT20-24F8
<400>672
atg ata gag gta aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
agc gat tta ctg cgt ggt gca ttt cac tta ggc ggc tac tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta ctt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Leu Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac atg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc ttc tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Phe Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>673
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_GE002278_GAT20-24F8
<220>
<223>合成的蛋白质序列
<400>673
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gl n Lys Gln Tyr Gln Leu Arg Gly Val Ala ThrLeu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Leu Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Phe Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>674
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_GE002279_GAT20-26A4
<400>674
atg ata gag gta aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta ctg cgt ggt gca ttt cac tta ggc ggc tac tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
aaa ctg att tcc atc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag att cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Ile Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac cta ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
ata ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>675
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_GE002279_GAT20-26A4
<220>
<223>合成的蛋白质序列
<400>675
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Ile Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>676
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_GE002280_GAT20-27C7
<400>676
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
agc gat tta ctg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aga ctg att tcc gtc gct tca ttc cac cag gcc gag cac acg gaa ctt 192
Arg Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac atg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc ttc tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Phe Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc ga cacg ccg cca gta gga cct cac atc atg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Met Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>677
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_GE002280_GAT20-27C7
<220>
<223>合成的蛋白质序列
<400>677
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Arg Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Ash Ala Arg Thr
100 105 110
Ser Ala Ser Gly Phe Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Met Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>678
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_GE002281_GAT20-27F9
<400>678
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
agc gat tta atg cgt ggt gca ttt cac tta ggc ggc tac tac ggg ggc 144
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
aga ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ttt 192
Arg Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Phe
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att ata cgt aag agg ggg gcg gac cta ctt tgg tgt aat gcg cgg aca 336
Ile Ile Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
ata ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>679
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_GE002281_GAT20-27F9
<220>
<223>合成的蛋白质序列
<400>679
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
Arg Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Phe
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Ile Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>680
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_GE002282_GAT20-30B10
<400>680
atg ata gag gta aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
agc gat tta ctg cgt ggt gca ttt cac tta ggc ggc tac tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
aaa ctg att tcc atc gct tca ttc cac cag gcc gag cac acg gaa ctt 192
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga act agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac atg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc ttc tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Phe Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>681
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_GE002282_GAT20-30B10
<220>
<223>合成的蛋白质序列
<400>681
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Phe Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>682
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_GE002283_GAT20-30C6
<400>682
atg ata gag gta aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta ctg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac acg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac ata ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Ile Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aaa aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>683
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_GE002283_GAT20-30C6
<220>
<223>合成的蛋白质序列
<400>683
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Ile Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>684
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_GE003198_GAT20-30C6b
<400>684
atg ata gag gta aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta ctg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac ata ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Ile Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aaa aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>685
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_GE003198_GAT20-30C6b
<220>
<223>合成的蛋白质序列
<400>685
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Ile Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>686
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_GE002284_GAT20-30F7
<400>686
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga atc ctc aga cca aac cag ccg ata gaa gcg tgt atc ttt gaa 96
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Ile Phe Glu
20 25 30
agc gat tta atg cgt ggt gca ttt cac tta ggc ggc tac tac ggg ggc 144
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
aaa ctg att tcc atc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac ata ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Ile Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aaa aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>687
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_GE002284_GAT20-30F7
<220>
<223>合成的蛋白质序列
<400>687
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Ile Phe Glu
20 25 30
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Ile Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>688
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_GE002285_GAT20-31D6
<400>688
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atc ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Ile Phe Asp
20 25 30
agc gat tta ctg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aga ctg att tcc atc gct tca ttc cac ccg gcc gag cac tcg gaa ctt 192
Arg Leu Ile Ser Ile Ala Ser Phe His Pro Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac cta ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc atg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Met Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>689
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_GE002285_GAT20-31D6
<220>
<223>合成的蛋白质序列
<400>689
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Ile Phe Asp
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Arg Leu Ile Ser Ile Ala Ser Phe His Pro Ala Glu His Set Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Met Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>690
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_GE002286_GAT20-31G1
<400>690
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atc ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Ile Phe Glu
20 25 30
agc gat tta ctg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac ctg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc ttc tac aaa aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Phe Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc atg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Met Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>691
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_GE002286_GAT20-31G1
<220>
<223>合成的蛋白质序列
<400>691
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Ile Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Phe Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Met Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>692
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00339957_GAT19-1A7
<400>692
atg ata gag gtg aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga ata ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta ctt cgt ggt gca ttt cac tta ggc ggc tat tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
aaa ctg att tcc ata gct tca ttc cac cag gcc gag cac tca gaa ctc 192
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctc cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aaa gcg gga tcg agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
atc ctc cgt aag agg ggg gcg gac ttg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aaa aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttt gat acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>693
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00339957_GAT19-1A7
<220>
<223>合成的蛋白质序列
<400>693
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>694
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00339989_GAT19-1D3
<400>694
atg ata gaa gtg aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga ata ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta ctt cgt ggt gca ttt cac tta ggc ggc ttt tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc ata gct tca ttc cac cag gcc gag cac tca gac ctc 192
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Asp Leu
50 55 60
caa ggc cag aaa cag tac cag ctc cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Le uGlu Gly
65 70 75 80
tat cgt gag cag aaa gcg gga tca act cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Val Lys His Ala Glu Glu
85 90 95
atc ctt cgt aag agg ggg gcg gac atg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aaa aag tta ggc ctc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Leu Ser Glu Gln Gly Glu
115 120 125
gta ttt gac acg ccg cca gta ggg cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
ctc aca taa 441
Leu Thr *
145
<210>695
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00339989_GAT19-1D3
<220>
<223>合成的蛋白质序列
<400>695
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Asp Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Leu Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Leu Thr
145
<210>696
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00339991_GAT19-1D5
<400>696
atg ata gaa gtg aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aaa ata ctc aga cca aac cag ccg tta gaa gtg tgt atg ttt gaa 96
His Lys Ile Leu Arg Pro Asn Gln Pro Leu Glu Val Cys Met Phe Glu
20 25 30
agc gat tta ctt cgc ggt gca ttt cac tta ggc ggc tat tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
aaa ctg att tcc ata gct tca ttc cac cag gcc gag cac tcg gaa ctc 192
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctc cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aaa gcg gga tcg agt cta att aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
att ctt cgt aag agg ggg gtg gac atg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Val Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aaa aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
ctc aca taa 441
Leu Thr *
145
<210>697
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00339991_GAT19-1D5
<220>
<223>合成的蛋白质序列
<400>697
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Leu Glu Val Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Val Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Leu Thr
145
<210>698
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00340001_GAT19-1F3
<400>698
atg ata gaa gtg aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aaa ata ctc aga cca aac cag ccg tta gaa gtg tgt atg tat gaa 96
His Lys Ile Leu Arg Pro Asn Gln Pro Leu Glu Val Cys Met Tyr Glu
20 25 30
acc gat tta ctt cgt ggc gca ttt cac tta ggc ggc ttt tac ggg ggc 144
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc ata gct tca ttc cac cag gcc gag cac tca gac ctc 192
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Asp Leu
50 55 60
caa ggc cag aaa cag tac cag ctc cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gat cag aaa gcg gga tcg agt cta att aaa cac gct gaa gaa 288
Tyr Arg Asp Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
att ctt cgt aag agg ggg gcg gac atg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aaa aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>699
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00340001_GAT19-1F3
<220>
<223>合成的蛋白质序列
<400>699
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Leu Glu Val Cys Met Tyr Glu
20 25 30
Thr Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Asp Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Asp Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>700
<211>441
<212> DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00340013_GAT19-1G3
<400>700
atg ata gag gtg aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga ata ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta ctt cgt ggt gca ttt cac tta ggc ggc ttt tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc ata gct tca ttc cac cag gcc gag cac tca gaa ctc 192
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctc cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aaa gcg gga tca act cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Val Lys His Ala Glu Glu
85 90 95
atc ctt cgt aag agg ggg gcg gac atg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aaa aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttt gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>701
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00340013_GAT19-1G3
<220>
<223>合成的蛋白质序列
<400>701
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>702
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00340017_GAT19-1G7
<400>702
atg ata gag gtg aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga ata ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta ctt cgt ggt gca ttt cac tta ggc ggc tat tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
aaa ctg att tcc ata gct tca ttc cac cag gcc gag cac tcg gaa ctc 192
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctc cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aaa gcg gga tca act cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Val Lys His Ala Glu Glu
85 90 95
atc ctt cgt aag agg ggg gcg gac atg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aaa aag tta ggc ttc ggc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Gly Glu Gln Gly Glu
115 120 125
gta ttt gaa acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
I1e Thr *
145
<210>703
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00340017_GAT19-1G7
<220>
<223>合成的蛋白质序列
<400>703
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Gly Glu Gln Gly Glu
115 120 125
Val Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>704
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00340201_GAT19-3H11
<400>704
atg ata gaa gtg aaa ccg att aac gca gag gat acc tat gag cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga ata ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta ctt cgt ggt gca ttt cac tta ggc ggc ttt tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc ata gct tca ttc cac cag gcc gag cac tcg gaa ctc 192
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctc cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aaa gcg gga tca act cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Val Lys His Ala Glu Glu
85 90 95
att ctt cgt aag agg ggg gcg gac ttg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Ash Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aaa aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttt gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>705
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00340201_GAT19-3H1l
<220>
<223>合成的蛋白质序列
<400>705
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>706
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00340360_GAT19-5H2
<400>706
atg ata gag gtg aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga ata ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta ctt cgt ggt gca ttt cac tta ggc ggc tat tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
aaa ctg att tcc ata gct tca ttc cac cag gcc gag cac tca gaa ctc 192
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctc cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gat cag aaa gcg gga tcg agt cta gtt aga cac gct gaa caa 288
Tyr Arg Asp Gln Lys Ala Gly Ser Ser Leu Val Arg His Ala Glu Gln
85 90 95
att ctt cgt aag agg ggg gcg gac ttg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aaa aag tta ggc ttc agt gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
ctc aca taa 441
Leu Thr *
145
<210>707
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00340360_GAT19-5H2
<220>
<223>合成的蛋白质序列
<400>707
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Asp Gln Lys Ala Gly Ser Ser Leu Val Arg His Ala Glu Gln
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Leu Thr
145
<210>708
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00340389_GAT19-6B7
<400>708
atg ata gaa gtg aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga ata ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta ctt cgt ggt gca ttt cac tta ggc ggc tat tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
aaa ctg att tcc ata gct tca ttc cac cag gcc gag cac tca gaa ctc 192
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctc cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga tcg agt cta att aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
att ctt cgt aag agg ggg gcg gac atg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aaa aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
ata ttt gac acg ccg cca gta gga ccc cac atc ctg atg tat aaa agg 432
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
ate aca taa 441
Ile Thr *
145
<210>709
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00340389_GAT19-6B7
<220>
<223>合成的蛋白质序列
<400>709
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>710
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00340420_GAT19-6F2
<400>710
atg ata gaa gtg aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga ata ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat ttg ctt cgt ggt gca ttt cac tta ggc ggc ttt tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc ata gct tca ttc cac cag gct gag cac tca gaa ctc 192
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctc cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aaa gcg gga tcg agt cta att aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
atc ctt cgt aag agg ggg gcg gac ttg ctt tgg tgt aat gcg cgg gca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Ala
100 105 110
tcc gcc tca ggc tac tac aaa aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttt gat acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
ctc aca taa 441
Leu Thr *
145
<210>711
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00340420_GAT19-6F2
<220>
<223>合成的蛋白质序列
<400>711
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Ala
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Leu Thr
145
<210>712
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00340423_GAT19-6F5
<400>712
atg ata gaa gtg aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga ata ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta ctt cgt ggt gca ttt cac tta ggc ggc tat tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
aaa ctg att tcc ata gct tca ttc cac cag gcc gag cac tcg gaa ctc 192
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctc cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga tcg agt cta att aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
att ctt cgt aag agg ggg gcg gac atg ctt tgg tgc aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aaa aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttt gaa acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>713
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00340423_GAT19-6F5
<220>
<223>合成的蛋白质序列
<400>713
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 1l0
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>714
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00340426_GAT19-6F8
<400>714
atg ata gag gtg aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga ata ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta ctt cgt ggt gca ttt cac tta ggc ggc ttt tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc ata gct tca ttc cac cag gct gag cac tca gaa ctc 192
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctc cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aaa gcg gga tcg agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
atc ctt cgt aag agg ggg gcg gac atg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aaa aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttt gaa acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>715
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00340426_GAT19-6F8
<220>
<223>合成的蛋白质序列
<400>715
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>716
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00340428_GAT19-6F10
<400>716
atg ata gag gtg aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aaa ata ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Lys Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta ctt cgt ggt gca ttt cac tta ggc ggc ttt tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc ata gct tca ttc cac cag gcc gag cac tcg gaa ctc 192
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctc cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Len Glu Gly
65 70 75 80
tat cgt gag cag aaa gcg ggg tcg agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
atc ctt cgt aag agg ggg gcg gac atg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aag aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttt gaa acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>717
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S0034042_8GAT19-6F10
<220>
<223>合成的蛋白质序列
<400>717
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Gln Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>718
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00340436_GAT19-6G6
<400>718
atg ata gaa gtg aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aaa ata ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Lys Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta ctt cgt ggt gca ttt cac tta ggc ggc ttt tac agg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
aaa ctg att tcc ata gct tca ttc cac cag gcc gag cac tcg gaa ctc 192
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctc cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aaa gcg gga tcg agt cta att aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
atc ctt cgt aag agg ggg gcg gac atg ctt tgg tgc aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aaa aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttt gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>719
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00340436_GAT19-6G6
<220>
<223>合成的蛋白质序列
<400>719
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>720
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00340437_GAT19-6G7
<400>720
atg ata gag gtg aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga ata ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta ctt cgt ggt gca ttt cac tta ggc ggc tat tac agg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
aaa ctg gtt tcc ata gct tca ttc cac cag gcc gag cac tca gac ctc 192
Lys Leu Val Ser lle Ala Ser Phe His Gln Ala Glu His Ser Asp Leu
50 55 60
caa ggc cag aaa cag tac cag ctc cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga tcg agt cta att aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
att ctt cgt aag agg ggg gcg gac atg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aaa aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttt gaa acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
ctc aca taa 441
Leu Thr *
145
<210>721
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00340437_GAT19-6G7
<220>
<223>合成的蛋白质序列
<400>721
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Val Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Asp Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Glu Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Leu Thr
145
<210>722
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00341190_GAT20-1F8
<400>722
atg ata gag gta aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
agc gat tta ctg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aga ctg att tcc gtc gct tca ttc cac cag gcc gag cac acg gaa ctt 192
Arg Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac ctg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>723
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00341190_GAT20-1F8
<220>
<223>合成的蛋白质序列
<400>723
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Arg Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>724
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00341206_GAT20-1G12
<400>724
atg ata gag gta aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta ctg cgt ggt gca ttt cac tta ggc ggc tac tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
aaa ctg att tcc atc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag att cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Ile Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac cta ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 25
ata ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>725
<21l>146
<212>PRT
<213>人工序列
<220>
<223>D_S00341206_GAT20-1G12
<220>
<223>合成的蛋白质序列
<400>725
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Ile Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>726
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00341290_GAT20-2G12
<400>726
atg ata gag ata aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Ile Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
agc gat tta atg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt act agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac atg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aaa aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc atg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Met Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>727
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00341290_GAT20-2G12
<220>
<223>合成的蛋白质序列
<400>727
Met Ile Glu Ile Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Met Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>728
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00341421_GAT20-4C11
<400>728
atg ata gag gta aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atc ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Ile Phe Glu
20 25 30
agc gat tta ctg cgt ggt gca ttt cac tta ggc ggc tac tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
aaa ctg ata tcc atc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac cta ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag caa gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc atg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Met Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>729
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00341421_GAT20-4C11
<220>
<223>合成的蛋白质序列
<400>729
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Ile Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 10
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Met Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>730
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00341448_GAT20-4G2
<400>730
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
agc gat tta ctg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aga ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Arg Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att ata cgt aag agg ggg gcg gac ctg ctt tgg tgt aat gcg cgg aca 336
Ile Ile Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc atg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Met Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>731
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00341448_GAT20-4G2
<220>
<223>合成的蛋白质序列
<400>731
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Arg Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Ile Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Met Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>732
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00341692_GAT20-7F6
<400>732
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
agc gat tta ctg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac ttg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Leu Glu Leu
50 55 60
caa ggc cag aaa cag tac cag att cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Ile Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac ctg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
ata ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>733
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00341692_GAT20-7F6
<220>
<223>合成的蛋白质序列
<400>733
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Leu Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Ile Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>734
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00341732_GAT20-8A10
<400>734
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga atc ctc aga cca aac cag ccg ata gaa gcg tgt atc ttt gaa 96
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Ile Phe Glu
20 25 30
agc gat tta atg cgt ggt gca ttt cac tta ggc ggc tac tac ggg ggc 144
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
aaa ctg att tcc atc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac ata ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Ile Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aaa aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>735
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00341732_GAT20-8A10
<220>
<223>合成的蛋白质序列
<400>735
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Ile Phe Glu
20 25 30
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Ile Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>736
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00341794_GAT20-8G12
<400>736
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cra agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta atg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa att 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Ile
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac cta ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
ata ttc gac acg ccg cca gta gga cct cac atc atg atg tat aaa agg 432
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Met Met Tyr Lys Arg
130 135 140
arc aca taa 441
Ile Thr *
145
<210>737
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00341794_GAT20-8G12
<220>
<223>合成的蛋白质序列
<400>737
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Ile
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 10
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Met Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>738
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00341806_GAT20-8H12
<400>738
atg ata gag gta aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ecg ata gaa gcg tgt atc ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Ile Phe Glu
20 25 30
agc gat tta atg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aga ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Arg Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac cta ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aaa aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>739
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00341806_GAT20-8H12
<220>
<223>合成的蛋白质序列
<400>739
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Ile Phe Glu
20 25 30
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Arg Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 25
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>740
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00375329_GAT20-8H12b
<400>740
atg ata gag gta aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atc ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Ile Phe Glu
20 25 30
agc gat tta atg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aga ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Arg Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac cta ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aaa aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>741
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00375329_GAT20-8H12b
<220>
<223>合成的蛋白质序列
<400>741
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Ile Phe Glu
20 25 30
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Arg Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>742
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00341822_GAT20-9B4
<400>742
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
agc gat tta ctg cgt ggt gca ttt cac tta ggc ggc tac tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
aga ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Arg Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac ata ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Ile Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gat 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Asp
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>743
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00341822_GAT20-9B4
<220>
<223>合成的蛋白质序列
<400>743
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
Arg Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Ile Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Asp
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>744
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00342013_GAT20-11D3
<400>744
atg ata gag gta aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atc ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Ile Phe Glu
20 25 30
agc gat tta atg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aga ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Arg Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac cta ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aaa aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>745
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00342013_GAT20-11D3
<220>
<223>合成的蛋白质序列
<400>745
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Ile Phe Glu
20 25 30
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Arg Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>746
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00342016_GAT20-11D6
<400>746
atg ata gag gta aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atc ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Ile Phe Glu
20 25 30
agc gat tta ctg cgt ggt gca ttt cac tta ggc ggc tac tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
aaa ctg ata tcc atc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac cta ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag caa gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc atg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Met Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>747
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00342016_GAT20-11D6
<220>
<223>合成的蛋白质序列
<400>747
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Ile Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Tyr Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Met Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>748
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00392016_GAT22-1C12
<400>748
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
agc gat tta atg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aga ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Arg Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt act agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gtg gac cta ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Val Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
ata ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>749
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00392016_GAT22-1C12
<220>
<223>合成的蛋白质序列
<400>749
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Arg Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Val Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>750
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00392026_22-1D10
<400>750
atg ata gag ata aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Ile Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta atg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac cta ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 44l
Ile Thr *
145
<210>751
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00392026_22-1D10
<220>
<223>合成的蛋白质序列
<400>751
Met Ile Glu Ile Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>752
<211>441
<212>DNA
<2l3>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00392111_GAT22-2D11
<400>752
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta atg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gtg gac ctg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Val Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>753
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00392111_GAT22-2D11
<220>
<223>合成的蛋白质序列
<400>753
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Val Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>754
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00392304_GAT22-4G12
<400>754
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
agc gat tta atg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac ata ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Ile Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc ga cacg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>755
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00392304_GAT22-4G12
<220>
<223>合成的蛋白质序列
<400>755
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Ile Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>756
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00392376_GAT22-5F12
<400>756
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atc ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Ile Phe Glu
20 25 30
agt gat tta atg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac ctg ctt tgg tgt aat gcgc gg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>757
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00392376_GAT22-5F12
<220>
<223>合成的蛋白质序列
<400>757
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Ile Phe Glu
20 25 30
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>758
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00392449_GAT22-6F1
<400>758
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta ctg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt act agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac atg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>759
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00392449_GAT22-6F1
<220>
<223>合成的蛋白质序列
<400>759
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>760
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00392474_GAT22-6H2
<400>760
atg ata gag gta aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
agc gat tta atg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac atg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>761
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00392474_GAT22-6H2
<220>
<223>合成的蛋白质序列
<400>761
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>762
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00392595_GAT22-8C3
<400>762
atg ata gag gta aaa ccg att aac gea gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atc ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Ile Phe Glu
20 25 30
agc gat tta atg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aga ctg att tcc gtc gct tca ttc cae cag gcc gag cac tcg gaa ctt 192
Arg Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gtg gac ata ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Val Asp Ile Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>763
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00392595_GAT22-8C3
<220>
<223>合成的蛋白质序列
<400>763
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Ile Phe Glu
20 25 30
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Arg Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Val Asp Ile Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>764
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00392619_22-8F3
<400>764
atg ata gag ata aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Ile Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta ctg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt act agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac cta ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>765
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00392619_22-8F3
<220>
<223>合成的蛋白质序列
<400>765
Met Ile Glu Ile Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>766
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<22l>CDS
<222>(1)...(441)
<220>
<223>D_S00392706_GAT22-9F6
<400>766
atg ata gag gta aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
agc gat tta ctg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aga ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Arg Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac ctg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>767
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00392706_GAT22-9F6
<220>
<223>合成的蛋白质序列
<400>767
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Arg Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>768
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00392922_22-12B6
<400>768
atg ata gag gta aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
agc gat tta atg cgt ggt gca ttg cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Met Arg Gly Ala Leu His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac atg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aaa aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>769
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00392922_22-12B6
<220>
<223>合成的蛋白质序列
<400>769
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Ser Asp Leu Met Arg Gly Ala Leu His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>770
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00397347_21-15C11
<400>770
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
agc gat tta atg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac cta ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>771
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00397347_21-15C11
<220>
<223>合成的蛋白质序列
<400>771
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>772
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00397467_21-16G11
<400>772
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta atg cgt ggt gca ttt cac tta ggt ggc ttc tac ggg ggc 144
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac atg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>773
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00397467_21-16G11
<220>
<223>合成的蛋白质序列
<400>773
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>774
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00397517_GAT21-17D1
<400>774
atg ata gag gta aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta atg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Met Arg G1y Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc atc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt gtg gct acc tta gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac atg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>775
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00397517_GAT21-17D1
<220>
<223>合成的蛋白质序列
<400>775
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>776
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00397574_GAT21-18A10
<400>776
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
agc gat tta atg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac atg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aaa aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta ggg cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>777
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00397574_GAT21-18A10
<220>
<223>合成的蛋白质序列
<400>777
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>778
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00397585_GAT21-18B9
<400>778
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
agc gat tta atg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt atg gct acc tta gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac cta ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>779
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00397585_GAT21-18B9
<220>
<223>合成的蛋白质序列
<400>779
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>780
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00397672_22-13B12
<400>780
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta atg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac ata ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Ile Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcg gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>781
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00397672_22-13B12
<220>
<223>合成的蛋白质序列
<400>781
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Ile Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>782
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00397680_GAT22-13C8
<400>782
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtt ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
agc gat tta atg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gtg gac cta ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Val Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>783
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00397680_GAT22-13C8
<220>
<223>合成的蛋白质序列
<400>783
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Val Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>784
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00397762_GAT22-14C6
<400>784
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atc ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Ile Phe Glu
20 25 30
agc gat tta ctg cgc ggt gca ttt cac tta ggg ggc ttc tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt act agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac atg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>785
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00397762_GAT22-14C6
<220>
<223>合成的蛋白质序列
<400>785
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Ile Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>786
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00397779_GAT22-14D11
<400>786
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
agt gat tta atg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac cta ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc cta atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>787
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00397779_GAT22-14D11
<220>
<223>合成的蛋白质序列
<400>787
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>788
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00397832_GAT22-15B4
<400>788
atg ata gag gta aaa ccg att aac gca gaa gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
agc gat tta atg cgt agt gca ttt cae tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Met Arg Ser Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac acg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gtg gac cta ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Val Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aaa aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>789
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00397832_GAT22-15B4
<220>
<223>合成的蛋白质序列
<400>789
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Ser Asp Leu Met Arg Ser Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Val Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>790
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00397844_GAT22-15C4
<400>790
atg ata gag gta aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta ctg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt act agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac ctg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>791
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00397844_GAT22-15C4
<220>
<223>合成的蛋白质序列
<400>791
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>792
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00397944_22-16D8
<400>792
atg ata gag gta aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta ctg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac acg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac atg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttt agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>793
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00397944_22-l6D8
<220>
<223>合成的蛋白质序列
<400>793
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>794
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00398097_GAT22-18C5
<400>794
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta acg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tat cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gtg gac cta ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Val Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tea ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>795
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00398097_GAT22-18C5
<220>
<223>合成的蛋白质序列
<400>795
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Val Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>796
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00398147_22-18H7
<400>796
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atc ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Ile Phe Glu
20 25 30
agc gat tta atg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
cag ggc cag aaa cag tac cag ctt cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac atg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aga agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Arg Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>797
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00398147_22-18H7
<220>
<223>合成的蛋白质序列
<400>797
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Ile Phe Glu
20 25 30
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 20 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Arg Arg
130 135 140
Ile Thr
145
<210>798
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00398975_21-19F1
<400>798
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atc ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Ile Phe Glu
20 25 30
agc gat tta ctg cgt ggt gca ttt cac cta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac ctg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg cca cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>799
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00398975_21-19F1
<220>
<223>合成的蛋白质序列
<400>799
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Ile Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>800
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00398977_21-19F3
<400>800
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta ctg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac ata ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Ile Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aaa aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
ata ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>801
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00398977_21-19F3
<220>
<223>合成的蛋白质序列
<400>801
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Ile Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>802
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00399101_GAT21-21A7
<400>802
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
agc gat tta atg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac acg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac ata ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Ile Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>803
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00399101_GAT21-21A7
<220>
<223>合成的蛋白质序列
<400>803
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Ile Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>804
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00399539_21-26C1
<400>804
atg ara gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta atg egt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtt gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac ctg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aaa aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>805
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00399539_21-26C1
<220>
<223>合成的蛋白质序列
<400>805
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>806
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00399765_21-28H11
<400>806
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
agc gat tta atg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag att cga ggt gtg gct acc tta gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Ile Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac atg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aaa aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>807
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00399765_21-28H11
<220>
<223>合成的蛋白质序列
<400>807
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Ile Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>808
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00400238_GAT22-19F1
<400>808
atg ata gag gta aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
agc gat tta atg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc atc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gtg gac cta ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Val Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
ata ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>809
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00400238_GAT22-19Fl
<220>
<223>合成的蛋白质序列
<400>809
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Val Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>810
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00400319_GAT22-20D10
<400>810
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta atg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt act agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gtg gaG cta ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Val Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>811
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00400319_GAT22-20D10
<220>
<223>合成的蛋白质序列
<400>811
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Val Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>812
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>D_S00401090_GAT22-29G1
<400>812
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta ctg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aga ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Arg Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt atg gct acc ttg gaa ggc 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac ata ctt tgg tgc aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Ile Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aaa aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>813
<211>146
<212>PRT
<213>人工序列
<220>
<223>D_S00401090_GAT22-29G1
<220>
<223>合成的蛋白质序列
<400>813
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Arg Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Ile Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>814
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>18-28D9b
<400>814
atg ata gag gtg aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga ata ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta ctt cgt ggt gca ttt cac tta ggc ggc ttt tac agg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
aaa ctg att tcc ata gct tca ttc cac cag gcc gag cac tcg gaa ctc 192
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctc cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aaa gcg gga tca act cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Val Lys His Ala Glu Glu
85 90 95
atc ctt cgt aag agg ggg gcg gac atg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aaa aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
ata ttt gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>815
<211>146
<212>PRT
<213>人工序列
<220>
<223>18-28D9b
<220>
<223>合成的蛋白质序列
<400>815
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>816
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>20-8H12c
<400>816
atg ata gag gtg aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atc ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Ile Phe Glu
20 25 30
agc gat tta atg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aga ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Arg Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac cta ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aaa aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>817
<211>146
<212>PRT
<213>人工序列
<220>
<223>20-8H12c
<220>
<223>合成的蛋白质序列
<400>817
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Ile Phe Glu
20 25 30
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Arg Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>818
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>20-16A3c
<400>818
atg ata gag gtg aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
agc gat tta ctg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aga ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Arg Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac ctg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>819
<211>146
<212>PRT
<213>人工序列
<220>
<223>20-16A3c
<220>
<223>合成的蛋白质序列
<400>819
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Arg Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>820
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>20-30C6c
<400>820
atg ata gag gtg aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
l 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta ctg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac ata ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Ile Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aaa aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>821
<211>146
<212>PRT
<213>人工序列
<220>
<223>20-30C6c
<220>
<223>合成的蛋白质序列
<400>821
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Ile Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>822
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>11-3C3b
<400>822
atg ara gag gtg aaa ccg att aac gca gag gat acc tat gaa cra agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aaa ata ctc aga cca aac cag ccg ata gaa gcg tgt atg tat gaa 96
His Lys Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Tyr Glu
20 25 30
agc gat tta ctt cgt ggt gca ctt cac tta ggc ggc tat tac agg ggc 144
Ser Asp Leu Leu Arg Gly Ala Leu His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
aaa ctg att tcc ata gcg tca ttc cac cag gcc gag cac tca gaa ctc 192
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctc cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aaa gcg gga tcg agt cta att aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
att ctt cgt aag agg ggg gcg gac ttg ctt tgg tgc aat gcg egg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aaa aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttt gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>823
<211>146
<212>PRT
<213>人工序列
<220>
<223>11-3C3b
<220>
<223>合成的蛋白质序列
<400>823
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Lys Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Tyr Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Leu His Leu Gly Gly Tyr Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Ile Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>824
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<220>
<223>18-28D9c
<400>824
atg ata gag gtg aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga ata ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta ctt cgt ggt gca ttt cac tta ggc ggc ttt tac agg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
aaa ctg att tcc ata gct tca ttc cac cag gcc gag cac tcg gaa ctc 192
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctc cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aaa gcg gga tca act cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Val Lys His Ala Glu Glu
85 90 95
atc ctt cgt aag agg ggg gcg gac atg ctt tgg tgt aat gcg agg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aaa aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
ata ttt gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>825
<211>146
<212>PRT
<213>人工序列
<220>
<223>18-28D9c
<220>
<223>合成的蛋白质序列
<400>825
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Ile Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Arg Gly
35 40 45
Lys Leu Ile Ser Ile Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Thr Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Ile Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>826
<211>15
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<400>826
atgattgaag tcaaa 15
<210>827
<211>15
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<400>827
agtatcgagg tgaag 15
<210>828
<211>5
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<400>828
Met Ile Glu Val Lys
1 5
<210>829
<211>10
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<400>829
Arg Pro Asn Gln Pro Leu Glu Ala Cys Met
1 5 10
<210>830
<211>10
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<400>830
Lys Pro Gln Gln Pro Val Glu Ser Cys Met
1 5 10
<210>831
<211>10
<212>PRT
<213>人工序列
<220>
<223>合成的蛋白质序列
<400>831
Lys Pro Asn Asn Pro Leu Asp Ala Cys Val
1 5 10
<210>832
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<223>GAT23-1F7
<400>832
atg ata gag gta aaa ccg att aac gca gaa gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta acg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac acg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
caa ggc cag aaa cag tat cag ctt cga ggt gtg gct acc ttg gag ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agc cta gtt aaa cgc gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys Arg Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac atg ctt tgg tgc aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcg gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>833
<211>146
<212>PRT
<213>人工序列
<220>
<223>23-1F7
<400>833
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys Arg Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>834
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<223>GAT23-2H1
<400>834
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
agc gat tta acg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
cag ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gag ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac atg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga ccc cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>835
<211>146
<212>PRT
<213>人工序列
<220>
<223>23-2H1
<400>835
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Ser Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>836
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<223>GAT23-2H11
<400>836
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
ggc gat tta atg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Gly Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cat gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgc aag agg ggg gcg gac cta ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>837
<211>146
<212>PRT
<213>人工序列
<220>
<223>23-2H11
<400>837
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Gly Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>838
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<22l>CDS
<222>(1)...(441)
<223>GAT23-3H1
<400>838
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta acg cgt ggt gca ttt cac cta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac atg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>839
<211>146
<212>PRT
<213>人工序列
<220>
<223>23-3H1
<400>839
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>840
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<223>GAT23-5A3
<400>840
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
agt gat tta acg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tat cag ctt cga ggc gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gtg gac cta ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Val Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tcg ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>841
<211>146
<212>PRT
<213>人工序列
<220>
<223>23-5A3
<400>841
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Ser Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Val Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>842
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<223>GAT23-6A3
<400>842
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga ccg aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta atg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
cag ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac atg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>843
<211>146
<212>PRT
<213>人工序列
<220>
<223>23-6A3
<400>843
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>844
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<223>GAT23-6H10
<400>844
atg ata gag gta aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta acg cgt ggt gca ttt cac cta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac acg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac cta ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag ggg gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>845
<211>146
<212>PRT
<213>人工序列
<220>
<223>23-6H10
<400>845
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>846
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<223>GAT24-4D5
<400>846
atg ata gag gta aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
agc gat tta acg cgt ggt gcg ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cgg gcc gag cac acg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Arg Ala Glu His Thr Glu Leu
50 55 60
caa ggc cag aaa cag tat cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac cta ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag cta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>847
<211>146
<212>PRT
<213>人工序列
<220>
<223>24-4D5
<400>847
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Ser Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Arg Ala Glu His Thr Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 20 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>848
<21l>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<223>GAT24-5H5
<400>848
atg ata gag gta aaa ccg att aac gca gaa gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
aac gat tta atg cgt agt gca ttt cac tta ggc ggc ttc cac ggg ggc 144
Asn Asp Leu Met Arg Ser Ala Phe His Leu Gly Gly Phe His Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tat cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac atg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag ttg ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg ccg gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>849
<211>146
<212>PRT
<213>人工序列
<220>
<223>24-5H5
<400>849
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Asn Asp Leu Met Arg Ser Ala Phe His Leu Gly Gly Phe His Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>850
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<223>GAT23-13D7
<400>850
atg ata gag gta aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta acg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac agg gcc gag cac acg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Arg Ala Glu His Thr Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt act agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gtg gac cta ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Val Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aaa aag cta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>851
<211>146
<212>PRT
<213>人工序列
<220>
<223>23-13D7
<400>851
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Arg Ala Glu His Thr Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Val Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>852
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<223>GAT24-9G5
<400>852
atg ata gag gta aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta acg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa tac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys Tyr Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gtg gac ctg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Val Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gtg gga ccc cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 44l
Ile Thr *
145
<210>853
<211>146
<212>PRT
<213>人工序列
<220>
<223>24-9G5
<400>853
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys Tyr Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Val Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>854
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<223>GAT24-9H9
<400>854
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
agc gat tta acg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac ata ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Ile Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcg gcc tca ggc tac tac aaa aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg cct cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>855
<211>146
<212>PRT
<213>人工序列
<220>
<223>24-9H9
<400>855
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Ser Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Ile Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>856
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<223>GAT24-12A5
<400>856
atg ata gat gta aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Asp Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
agc gat tta acg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tat cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cac gcc gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac cta ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aaa aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Lau Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>857
<211>146
<212>PRT
<213>人工序列
<220>
<223>24-12A5
<400>857
Met Ile Asp Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Ser Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>858
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<223>GAT24-14B7
<400>858
atg ata gag gta aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta atg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
cag ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtc aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgc aag agg ggg gcg gac atg att tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Ile Trp Cys Asn Ala Arg Thr
100 105 110
tca gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>859
<2ll>146
<212>PRT
<213>人工序列
<220>
<223>24-14B7
<400>859
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Ile Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>860
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<223>GAT24-15B10
<400>860
atg ata gag gta aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta acg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac atg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 100
tcc gcc tca ggc tac tac aaa aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>861
<211>146
<212>PRT
<213>人工序列
<220>
<223>24-15B10
<400>861
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>862
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<223>GAT24-15C3
<400>862
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta acg cgt agt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Thr Arg Ser Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
cag ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac cta ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>863
<211>146
<212>PRT
<213>人工序列
<220>
<223>24-15C3
<400>863
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Thr Arg Ser Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>864
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<223>GAT24-16B1
<400>864
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg gta gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Val Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta acg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac cta ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gtg gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>865
<211>146
<212>PRT
<213>人工序列
<220>
<223>24-16B1
<400>865
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Val Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>866
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<223>GAT24-16H12
<400>866
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
agc gat tta atg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg gtt tcc gtc gct tca ttc cac cag gcc gag cac acg gaa ctt 192
Lys Leu Val Ser Val Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac atg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>867
<211>146
<212>PRT
<213>人工序列
<220>
<223>24-16H12
<400>867
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Val Ser Val Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>868
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<223>GAT24-17A10
<400>868
atg ata gag gta aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta atg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tac cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac ctg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>869
<211>146
<212>PRT
<213>人工序列
<220>
<223>24-17A10
<400> 869
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>870
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<223>GAT24-17G11
<400>870
atg ata gag gta aaa ccg att aac gca gag gat act tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
agc gat tta atg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg gtt tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Val Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac ata ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Ile Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>871
<211>146
<212>PRT
<213>人工序列
<220>
<223> 24-17G11
<400>871
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Val Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Ile Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>872
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<223>GAT24-19H3
<400>872
atg ata gag gta aaa ccg att aac gca gaa gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta ctg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gct gag cac acg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cra gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac cta ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttt agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>873
<211>146
<212>PRT
<213>人工序列
<220>
<223>24-19H3
<400>873
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>874
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<223>GAT23-14A3
<400>874
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta atg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac acg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
cag ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac cta ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aaa aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>875
<211>146
<212>PRT
<213>人工序列
<220>
<223>23-14A3
<400>875
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>876
<211>441
<212>DNA
<2l3>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<223>GAT23-14B4
<400>876
atg ata gag gta aaa ccg att aac gca gaa gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta acg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cgg gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Arg Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt ctg gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac atg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>877
<211>146
<212>PRT
<213>人工序列
<220>
<223>23-14B4
<400>877
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Arg Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>878
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<223>GAT23-15G3
<400>878
atg ata gag gta aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta acg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gtg gac cta ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Val Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac gtc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Val Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>879
<211>146
<212>PRT
<213>人工序列
<220>
<223>23-15G3
<400>879
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Val Asp Leu Leu Trp Cys Ash Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Val Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>880
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<223>GAT23-18G4
<400>880
atg ata gag gta aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta atg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cgc tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu Arg Ser Glu Leu
50 55 60
cag ggc cag aaa cag tac cag ctt cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac atg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gca gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Ala Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>881
<211>146
<212>PRT
<213>人工序列
<220>
<223>23-18G4
<400>881
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu Arg Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Ala Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>882
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<223>GAT23-18H5
<400>882
atg ata gag gta aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta acg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tat cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac atg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>883
<211>146
<212>PRT
<213>人工序列
<220>
<223>23-18H5
<400>883
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>884
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<223>GAT23-19C3
<400>884
atg ata gag gta aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta acg cgt ggt gca ttt cac tta ggc ggc ttc tat ggg ggc 144
Ser Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg atc tcc gtc gct tca ttc cac cag gcc ggg cac acg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Gly His Thr Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac ata ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Ile Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcg gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>885
<211>146
<212>PRT
<213>人工序列
<220>
<223>23-19C3
<400>885
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Gly His Thr Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Ile Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>886
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<223>GAT23-19F6
<400>886
atg ata gag gta aaa ccg att aac gca gaa gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
agc gat tta acg cgt agt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Thr Arg Ser Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac acg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
caa ggc cgg aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Arg Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gtg gac cta ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Val Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>887
<211>146
<212>PRT
<213>人工序列
<220>
<223>23-19F6
<400>887
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Ser Asp Leu Thr Arg Ser Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
Gln Gly Arg Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Val Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>888
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<223>GAT25-1D10
<400>888
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta acg cgt agt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Thr Arg Ser Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
cag ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac cta ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aaa aag cta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>889
<211>146
<212>PRT
<213>人工序列
<220>
<223>25-1D10
<400>889
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Thr Arg Ser Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>890
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<223>GAT26-2D9
<400>890
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta acg cgt agt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Thr Arg Ser Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
cag ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac atg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg ccg gta gga cct cac atc ctg atg tac aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>891
<211>146
<212>PRT
<213>人工序列
<220>
<223>26-2D9
<400>891
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Thr Arg Ser Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>892
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<223>GAT26-2H4
<400>892
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
aac gat tta acg cgt ggt gcg ttt cac tta ggc ggc ttc tac ggg ggc 144
Asn Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg atc tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tac cgt gag cag aag gcg ggt act agt cta gtc aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac atg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aaa aag cta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>893
<211>146
<212>PRT
<213>人工序列
<220>
<223>26-2H4
<400>893
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Asn Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Ash Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>894
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<223>GAT26-3B5
<400>894
atg ata gag gta aaa ccg att aac gca gaa gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cac aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta acg cgt ggt gca ttt cac tta ggc ggc ttc tat ggg ggc 144
Ser Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg atc tcc gtc gct tca ttc cac cag gct gag cac acg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtc aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gtg gac cta ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Val Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag ttg ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr*
145
<210>895
<211>146
<212>PRT
<213>人工序列
<220>
<223>26-3B5
<400>895
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Val Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>896
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<223>GAT26-8F11
<400>896
atg ata gag gta aaa ccg att aac gca gaa gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
aac gat tta acg cgt ggc gca ttt cac tta ggc ggc ttc tac ggg ggc l44
Asn Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc ggg cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Gly His Ser Glu Leu
50 55 60
cag ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Let Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtc aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac atg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag ttg ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>897
<211>146
<212>PRT
<213>人工序列
<220>
<223>26-8F11
<400>897
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Asn Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Gly His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>898
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<22l>CDS
<222>(1)...(441)
<223>GAT26-9G5
<400>898
atg ata gag gta aaa ccg att aac gca gaa gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gca gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Ala Ala Cys Met Phe Glu
20 25 30
agc gat tta acg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg gtt tcc gtc gct tcg ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Val Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac cta ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag ttg ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>899
<211>146
<212>PRT
<213>人工序列
<220>
<223>26-9G5
<400> 899
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Ala Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Val Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>900
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<223>GAT26-15Dl0
<400>900
atg ata gag gtg aaa ccg att aac gca gag gat acc tac gaa cta aga 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
agc gat tta acg cgt agt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Thr Arg Ser Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
cag ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac atg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag ttg ggc ttt agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>901
<211>146
<212>PRT
<213>人工序列
<220>
<223>26-15D10
<400>901
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Ser Asp Leu Thr Arg Ser Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>902
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<223>GAT26-20H11
<400>902
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
agc gat tta acg cgt agt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Thr Arg Ser Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
cag ggc cag aaa cag tat cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgc gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac cta ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac agg aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>903
<211>146
<212>PRT
<213>人工序列
<220>
<223>26-20H11
<400>903
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Ser Asp Leu Thr Arg Ser Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>904
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<223>GAT26-20H12
<400>904
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
ggc gac tta acg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Gly Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
cag ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtc aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
atc cta cgt aag agg ggg gcg gac atg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tca gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag ggg gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg ccg gtg gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>905
<211>146
<212>PRT
<213>人工序列
<220>
<223>26-20H12
<400>905
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Gly Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>906
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<223>GAT25-8H7
<400>906
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta acg cgt agt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Thr Arg Ser Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc aag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Lys Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac atg att tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Ile Trp Cys Asn Ala Arg Thr
100 105 110
tct gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>907
<211>146
<212>PRT
<213>人工序列
<220>
<223>25-8H7
<400>907
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Thr Arg Ser Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Lys Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Ile Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>908
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<223>GAT25-14C12
<400>908
atg ata gag gta aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta acg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg gtt tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Val Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att ctg cgt aag agg ggg gcg gac atg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>909
<211>146
<212>PRT
<213>人工序列
<220>
<223>25-14C12
<400>909
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Val Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>910
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<223>GAT25-17F3
<400>910
atg ata gag gta aaa ccg att aac gca gaa gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
agc gat tta acg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tat cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgc aag agg ggg gcg gac atg att tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Ile Trp Cys Asn Ala Arg Thr
100 105 110
tcg gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac gtc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Val Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>911
<211>146
<212>PRT
<213>人工序列
<220>
<223>25-17F3
<400>911
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Ser Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Ile Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Val Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>912
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<223>GAT25-19C8
<400>912
atg ata gag gta aaa ccg att aac gca gaa gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta acg cgt agt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Thr Arg Ser Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tat cag ctt cga ggc gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtc aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gtg gac cta ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Val Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta ggt cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>913
<211>146
<212>PRT
<213>人工序列
<220>
<223>25-19C8
<400>913
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Thr Arg Ser Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Val Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>914
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<223>GAT25-23C1
<400>914
atg ata gag gta aaa ecg att aac gca gaa gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta acg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac acg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
caa ggc cgg aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Arg Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tac cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys Hls Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac atg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg ccg gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>915
<211>146
<212>PRT
<213>人工序列
<220>
<223>25 23C1
<400>915
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
Gln Gly Arg Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>916
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<223>R12G1
<400>916
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta acg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac act gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
caa ggc aag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Lys Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac atg att tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Ile Trp Cys Asn Ala Arg Thr
100 105 110
tct gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>917
<211>146
<212>PRT
<213>人工序列
<220>
<223>R12G1
<400>917
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
Gln Gly Lys Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Ile Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>918
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<223>R12G2
<400>918
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 l0 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta acg cgt agt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Thr Arg Ser Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac act gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
caa ggc aag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Lys Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac atg att tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Ile Trp Cys Asn Ala Arg Thr
100 105 110
tct gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 44l
Ile Thr *
145
<210>919
<211>146
<212>PRT
<213>人工序列
<220>
<223>R12G2
<400>919
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Thr Arg Ser Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
Gln Gly Lys Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Ile Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>920
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<223>R12G3
<400>920
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
aac gat tta acg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Asn Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc aag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Lys Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac atg att tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Ile Trp Cys Asn Ala Arg Thr
100 105 110
tct gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>921
<211>146
<212>PRT
<213>人工序列
<220>
<223>R12G3
<400>921
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Asn Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Lys Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Ile Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>922
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<223>R12G4
<400>922
atg ata gag gta aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
agc gat tta acg cgt agt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Thr Arg Ser Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc aag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Lys Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac atg att tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Ile Trp Cys Asn Ala Arg Thr
100 105 110
tct gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>923
<211>146
<212>PRT
<213>人工序列
<220>
<223>R12G4
<400>923
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Ser Asp Leu Thr Arg Ser Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Lys Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Ile Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>924
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<223>R12G5
<400>924
atg ata gag gta aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
agc gat tta acg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc aag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Lys Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac atg att tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Ile Trp Cys Asn Ala Arg Thr
100 105 110
tct gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>925
<211>146
<212>PRT
<213>人工序列
<220>
<223>R12G5
<400>925
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Ser Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Lys Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Ile Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>926
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<223>R12G6
<400>926
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
aac gat tta acg cgt agt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Asn Asp Leu Thr Arg Ser Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc aag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Lys Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac atg att tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Ile Trp Cys Asn Ala Arg Thr
100 105 110
tct gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>927
<211>146
<212>PRT
<213>人工序列
<220>
<223>R12G6
<400>927
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Asn Asp Leu Thr Arg Ser Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Lys Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Ile Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>928
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<223>R12G7
<400>928
atg ata gag gta aaa ccg att aac gca gaa gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
aac gat tta atg cgt ggt gca ttt cac tta ggc ggc ttc cac ggg ggc 144
Asn Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe His Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac act gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
caa ggc cag aaa cag tat cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa Gac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac atg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag ttg ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg ccg gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>929
<211>146
<212>PRT
<213>人工序列
<220>
<223>R12G7
<400>929
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Asn Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe His Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phc His Gln Ala Glu His Thr Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>930
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列
<220>
<221>CDS
<222>(1)...(441)
<223>R12G8
<400>930
atg ata gag gta aaa ccg att aac gca gaa gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
aac gat tta atg cgt agt gca ttt cac tta ggc ggc ttc cac ggg ggc 144
Asn Asp Leu Met Arg Ser Ala Phe His Leu Gly Gly Phe His Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac act gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
caa ggc cag aaa cag tat cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac atg ctt tgg tgt aat gcg cgg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag ttg ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg ccg gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>931
<211>146
<212>PRT
<213>人工序列
<220>
<223>R12G8
<400>931
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Asn Asp Leu Met Arg Ser Ala Phe His Leu Gly Gly Phe His Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>932
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列GAT4604SR
<220>
<221>CDS
<222>(1)...(441)
<223>编码20 H812(SEQ 739)
<400> 932
atg ata gag gtg aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atc ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Ile Phe Glu
20 25 30
agc gat tta atg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aga ctg att tcc gtc gct tca ttc cac cag gcc gag cac tct gaa ctt 192
Arg Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt atg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Met Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac cta ctt tgg tgt aat gcg agg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aaa aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>933
<211>449
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列GAT4609SR
<220>
<221>CDS
<222>(1)...(441)
<223>编码22-18C5(SEQ 795)
<400>933
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta acg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tct gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tat cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gtg gac cta ctt tgg tgt aat gcg agg aca 336
Ile Leu Arg Lys Arg Gly Val Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa ggcgcgcc 449
Ile Thr *
145
<210>934
<211>449
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列GAT4610R
<220>
<221>CDS
<222>(1)...(441)
<223>编码22-16D8(SEQ 793)
<400>934
atg ata gag gta aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta ctg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Leu Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac acg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac atg ctt tgg tgt aat gcg agg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttt agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa ggcgcgcc 449
Ile Thr *
145
<210>935
<211>449
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列GAT4611R
<220>
<221>CDS
<222>(1)...(441)
<223>编码22-15B4(SEQ 789)
<400>935
atg ata gag gta aaa ccg att aac gca gaa gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
agc gat tta atg cgt agt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Met Arg Ser Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac acg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gtg gac cta ctt tgg tgt aat gcg agg aca 336
Ile Leu Arg Lys Arg Gly Val Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aaa aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa ggcgcgcc 449
Ile Thr *
145
<210>936
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列GAT4614SR
<220>
<221>CDS
<222>(1)...(441)
<223>编码24-5H5(SEQ 849)
<400>936
atg ata gag gta aaa ccg att aac gca gaa gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
aac gat tta atg cgt agt gca ttt cac tta ggc ggc ttc cac ggg ggc 144
Asn Asp Leu Met Arg Ser Ala Phe His Leu Gly Gly Phe His Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tct gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tat cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac atg ctt tgg tgt aat gcg agg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag ttg ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg ccg gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>937
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列GAT4614VSR
<220>
<221>CDS
<222>(1)...(441)
<223>编码24-5H5(SEQ 849)
<400>937
atg ata gag gtg aaa ccg att aac gca gaa gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gat 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
aac gat tta atg cgt agt gca ttt cac tta ggc ggc ttc cac ggg ggc 144
Asn Asp Leu Met Arg Ser Ala Phe His Leu Gly Gly Phe His Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tct gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tat cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac atg ctt tgg tgt aat gcg agg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag ttg ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg ccg gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>938
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列GAT4615R
<220>
<221>CDS
<222>(1)...(441)
<223>编码23-2H11(SEQ 837)
<400>938
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
ggc gat tta atg cgt ggt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Gly Asp Leu Met Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cat gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgc aag agg ggg gcg gac cta ctt tgg tgt aat gcg agg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>939
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列GAT4616R
<220>
<221>CDS
<222>(1)...(441)
<223>编码24-15C3(SEQ 863)
<400>939
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta acg cgt agt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Thr Arg Ser Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
cag ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg gga acc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Thr Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac cta ctt tgg tgt aat gcg agg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>940
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列GAT4617R
<220>
<221>CDS
<222>(1)...(441)
<223>编码23-6H10(SEQ 845)
<400>940
atg ata gag gta aaa ccg att aac gca gag gat acc tat gaa cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta acg cgt ggt gca ttt cac cta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac acg gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Thr Glu Leu
50 55 60
caa ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac cta ctt tgg tgt aat gcg agg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag ggg gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>941
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列GAT4618SR
<220>
<221>CDS
<222>(1)...(441)
<223>编码25-8H7(SEQ 907)
<400>941
atg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta acg cgt agt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Thr Arg Ser Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tct gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc aag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Lys Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac atg att tgg tgt aat gcg agg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Ile Trp Cys Asn Ala Arg Thr
100 105 110
tct gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>942
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列GAT4618VSR
<220>
<221>CDS
<222>(1)...(441)
<223>编码25-8H7(SEQ 907)
<400>942
atg ata gag gtg aaa ccg att aac gca gag gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta acg cgt agt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Thr Arg Ser Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tct gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc aag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa ggt 240
Gln Gly Lys Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gcg gac atg att tgg tgt aat gcg agg aca 336
Ile Leu Arg Lys Arg Gly Ala Asp Met Ile Trp Cys Asn Ala Arg Thr
100 105 110
tct gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>943
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列GAT4619SR
<220>
<221>CDS
<222>(1)...(441)
<223>编码25-19C8(SEQ 913)
<400>943
atg ata gag gta aaa ccg att aac gca gaa gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta acg cgt agt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Thr Arg Ser Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tct gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tat cag ctt cga ggc gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtc aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gtg gac cta ctt tgg tgt aat gcg agg aca 336
Ile Leu Arg Lys Arg Gly Val Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta ggt cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>944
<211>441
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列GAT4619VSR
<220>
<221>CDS
<222>(1)...(441)
<223>编码25-19C8(SEQ 913)
<400>944
atg ata gag gtg aaa ccg att aac gca gaa gat acc tat gac cta agg 48
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt gaa 96
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
agc gat tta acg cgt agt gca ttt cac tta ggc ggc ttc tac ggg ggc 144
Ser Asp Leu Thr Arg Ser Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tct gaa ctt 192
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
caa ggc cag aaa cag tat cag ctt cga ggc gtg gct acc ttg gaa ggt 240
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
tat cgt gag cag aag gcg ggt tcc agt cta gtc aaa cac gct gaa gaa 288
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
att cta cgt aag agg ggg gtg gac cta ctt tgg tgt aat gcg agg aca 336
Ile Leu Arg Lys Arg Gly Val Asp Leu Leu Trp Cys Asn Ala Arg Thr
100 105 110
tcc gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga gag 384
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
gta ttc gac acg ccg cca gta ggt cct cac atc ctg atg tat aaa agg 432
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys Arg
130 135 140
atc aca taa 441
Ile Thr *
145
<210>945
<211>452
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列GAT4611A
<220>
<221>CDS
<222>(1)...(444)
<223>编码22-15B4 M1MA
<400>945
atg gcg ata gag gta aaa ccg att aac gca gaa gat acc tat gac cta 48
Met Ala Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu
1 5 10 15
agg cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt 96
Arg His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe
20 25 30
gat agc gat tta atg cgt agt gca ttt cac tta ggc ggc ttc tac ggg 144
Asp Ser Asp Leu Met Arg Ser Ala Phe His Leu Gly Gly Phe Tyr Gly
35 40 45
ggc aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac acg gaa 192
Gly Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Thr Glu
50 55 60
ctt caa ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa 240
Leu Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu
65 70 75 80
ggt tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cac gct gaa 288
Gly Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu
85 90 95
gaa att cta cgt aag agg ggg gtg gac cta ctt tgg tgt aat gcg cgg 336
Glu Ile Leu Arg Lys Arg Gly Val Asp Leu Leu Trp Cys Asn Ala Arg
100 105 110
aca tcc gcc tca ggc tac tac aaa aag tta ggc ttc agc gag cag gga 384
Thr Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly
115 120 125
gag gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa 432
Glu Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys
130 135 140
agg atc aca taa ggcgcgcc 452
Arg Ile Thr *
145
<210>946
<211>147
<212>PRT
<213>人工序列
<220>
<223>22-15B4 M1MA
<400>946
Met Ala Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu
1 5 10 15
Arg His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe
20 25 30
Asp Ser Asp Leu Met Arg Ser Ala Phe His Leu Gly Gly Phe Tyr Gly
35 40 45
Gly Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Thr Glu
50 55 60
Leu Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu
65 70 75 80
Gly Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu
85 90 95
Glu Ile Leu Arg Lys Arg Gly Val Asp Leu Leu Trp Cys Asn Ala Arg
100 105 110
Thr Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln Gly
115 120 125
Glu Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys
130 135 140
Arg Ile Thr
145
<210>947
<211>455
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列GAT4611AA
<220>
<221>CDS
<222>(1)...(447)
<223>编码22-15B4 M1MAA
<400>947
atg gcg gcc ata gag gta aaa ccg att aac gca gaa gat acc tat gac 48
Met Ala Ala Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp
1 5 10 15
cta agg cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg 96
Leu Arg His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met
20 25 30
ttt gat agc gat tta atg cgt agt gca ttt cac tta ggc ggc ttc tac 144
Phe Asp Ser Asp Leu Met Arg Ser Ala Phe His Leu Gly Gly Phe Tyr
35 40 45
ggg ggc aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac acg 192
Gly Gly Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Thr
50 55 60
gaa ctt caa ggc cag aaa cag tac cag ctt cga ggt gtg gct acc ttg 240
Glu Leu Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu
65 70 75 80
gaa ggt tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cac gct 288
Glu Gly Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala
85 90 95
gaa gaa att cta cgt aag agg ggg gtg gac cta ctt tgg tgt aat gcg 336
Glu Glu Ile Leu Arg Lys Arg Gly Val Asp Leu Leu Trp Cys Asn Ala
100 105 110
cgg aca tcc gcc tca ggc tac tac aaa aag tta ggc ttc agc gag cag 384
Arg Thr Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln
115 120 125
gga gag gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat 432
Gly Glu Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr
130 135 140
aaa agg atc aca taa ggcgcgcc 455
Lys Arg Ile Thr *
145
<210>948
<211>148
<212>PRT
<213>人工序列
<220>
<223>22-15B4 M1MAA
<400>948
Met Ala Ala Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp
1 5 10 15
Leu Arg His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met
20 25 30
Phe Asp Ser Asp Leu Met Arg Ser Ala Phe His Leu Gly Gly Phe Tyr
35 40 45
Gly Gly Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Thr
50 55 60
Glu Leu Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu
65 70 75 80
Glu Gly Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala
85 90 95
Glu Glu Ile Leu Arg Lys Arg Gly Val Asp Leu Leu Trp Cys Asn Ala
100 105 110
Arg Thr Ser Ala Ser Gly Tyr Tyr Lys Lys Leu Gly Phe Ser Glu Gln
115 120 125
Gly Glu Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr
130 135 140
Lys Arg Ile Thr
145
<210>949
<211>444
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列GAT4618A
<220>
<221>CDS
<222>(1)...(444)
<223>编码25-8H7 M1MA
<400>949
atg gcg ata gag gta aaa ccg att aac gca gag gat acc tat gac cta 48
Met Ala Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu
1 5 10 15
agg cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt 96
Arg His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe
20 25 30
gaa agc gat tta acg cgt agt gca ttt cac tta ggc ggc ttc tac ggg 144
Glu Ser Asp Leu Thr Arg Ser Ala Phe His Leu Gly Gly Phe Tyr Gly
35 40 45
ggc aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tcg gaa 192
Gly Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu
50 55 60
ctt caa ggc aag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa 240
Leu Gln Gly Lys Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu
65 70 75 80
ggt tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cac gct gaa 288
Gly Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu
85 90 95
gaa att cta cgt aag agg ggg gcg gac atg att tgg tgt aat gcg cgg 336
Glu Ile Leu Arg Lys Arg Gly Ala Asp Met Ile Trp Cys Asn Ala Arg
100 105 110
aca tct gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga 384
Thr Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly
115 120 125
gag gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa 432
Glu Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys
130 135 140
agg atc aca taa 444
Arg Ile Thr *
145
<210>950
<211>147
<212>PRT
<213>人工序列
<220>
<223>25-8H7 M1MA
<400>950
Met Ala Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu
1 5 10 15
Arg His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe
20 25 30
Glu Ser Asp Leu Thr Arg Ser Ala Phe His Leu Gly Gly Phe Tyr Gly
35 40 45
Gly Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu
50 55 60
Leu Gln Gly Lys Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu
65 70 75 80
Gly Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu
85 90 95
Glu Ile Leu Arg Lys Arg Gly Ala Asp Met Ile Trp Cys Asn Ala Arg
100 105 110
Thr Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly
115 120 125
Glu Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys
130 135 140
Arg Ile Thr
145
<210>951
<211>444
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列GAT4620
<220>
<221>CDS
<222>(1)...(444)
<223>编码25-8H7 M1MA(SEQ ID N0:950)
<400>951
atg gct att gag gtt aaa cct att aac gca gag gat acc tat gac cta 48
Met Ala Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu
1 5 10 15
agg cat aga gtc ctc aga cca aac cag ccg ata gaa gcg tgt atg ttt 96
Arg His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe
20 25 30
gaa agc gat tta acg cgt agt gca ttt cac tta ggc ggc ttc tac ggg 144
Glu Ser Asp Leu Thr Arg Ser Ala Phe His Leu Gly Gly Phe Tyr Gly
35 40 45
ggc aaa ctg att tcc gtc gct tca ttc cac cag gcc gag cac tct gaa 192
Gly Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu
50 55 60
ctt caa ggc aag aaa cag tac cag ctt cga ggt gtg gct acc ttg gaa 240
Leu Gln Gly Lys Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu
65 70 75 80
ggt tat cgt gag cag aag gcg ggt tcc agt cta gtt aaa cac gct gaa 288
Gly Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu
85 90 95
gaa att cta cgt aag agg ggg gcg gac atg att tgg tgt aat gcg agg 336
Glu Ile Leu Arg Lys Arg Gly Ala Asp Met Ile Trp Cys Asn Ala Arg
100 105 110
aca tct gcc tca ggc tac tac aga aag tta ggc ttc agc gag cag gga 384
Thr Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly
115 120 125
gag gta ttc gac acg ccg cca gta gga cct cac atc ctg atg tat aaa 432
Glu Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys
130 135 140
agg atc aca taa 444
Arg Ile Thr *
145
<210>952
<211>444
<212>DNA
<213>人工序列
<220>
<223>合成的核酸序列GAT4621
<220>
<221>CDS
<222>(1)...(444)
<223>编码25-8H7 M1MA(SEQ ID N0:950)
<400>952
atg gct att gag gtt aag cct atc aac gca gag gat acc tat gac ctt 48
Met Ala Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu
1 5 10 15
agg cat aga gtg ctc aga cca aac cag cct atc gaa gcc tgc atg ttt 96
Arg His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe
20 25 30
gag tct gac ctt act agg agt gca ttt cac ctt ggt gga ttc tac gga 144
Glu Ser Asp Leu Thr Arg Ser Ala Phe His Leu Gly Gly Phe Tyr Gly
35 40 45
ggt aaa ctg att tcc gtg gct tca ttc cac caa gct gag cac tct gaa 192
Gly Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu
50 55 60
ctt caa ggt aag aag cag tac cag ctt aga ggt gtg gct acc ttg gaa 240
Leu Gln Gly Lys Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu
65 70 75 80
ggt tat aga gag cag aag gct ggt tcc agt ctc gtg aaa cac gct gaa 288
Gly Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu
85 90 95
gag att ctc aga aag aga ggt gct gac atg atc tgg tgt aat gcc agg 336
Glu Ile Leu Arg Lys Arg Gly Ala Asp Met Ile Trp Cys Asn Ala Arg
100 105 110
aca tct gct tca gga tac tac agg aag ttg gga ttc agt gag caa gga 384
Thr Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly
115 120 125
gag gtg ttc gat act cct cca gtt gga cct cac atc ctg atg tat aag 432
Glu Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu Met Tyr Lys
130 135 140
agg atc aca taa 444
Arg Ile Thr *
145
<210>953
<211>146
<212>PRT
<213>人工序列
<220>
<223>fil 3 con
<221>变体
<222>4
<223>Val或Ile
<221>变体
<222>14
<223>Asp或Glu
<221>变体
<222>18
<223>Arg或Lys
<221>变体
<222>19
<223>Val或Ile
<221>变体
<222>26
<223>Ile或Leu或Val
<221>变体
<222>28
<223>Ala或Val
<221>变体
<222>30
<223>Met或Ile
<221>变体
<222>31
<223>Phe或Tyr
<221>变体
<222>32
<223>Glu或Asp
<221>变体
<222>33
<223>Ser或Asn
<221>变体
<222>36
<223>Thr或Met或Leu
<221>变体
<222>38
<223>Gly或Ser
<221>变体
<222>45
<223>Phe或Tyr
<221>变体
<222>47
<223>Gly或Arg
<221>变体
<222>49
<223>Lys或Arg
<221>变体
<222>51
<223>Ile或Val
<221>变体
<222>53
<223>Val或Ile
<221>变体
<222>58
<223>Gln或Arg
<221>变体
<222>60
<223>Glu或Gly
<221>变体
<222>62
<223>Ser或Thr
<221>变体
<222>63
<223>Glu或Asp
<221>变体
<222>64
<223>Leu或Ile
<221>变体
<222>67
<223>Gln或Lys或Arg
<221>变体
<222>72
<223>Leu或Ile
<221>变体
<222>75
<223>Val或Met
<221>变体
<222>83
<223>Asp或Glu
<221>变体
<222>88
<223>Ser或Thr
<221>变体
<222>89
<223>Ser或Thr
<221>变体
<222>91
<223>Val或Ile或Leu
<221>变体
<222>92
<223>Lys或Arg
<221>变体
<222>98
<223>Leu或Ile
<221>变体
<222>103
<223>Ala或Val
<221>变体
<222>105
<223>Met或Leu或Ile
<221>变体
<222>106
<223>Ile或Leu
<221>变体
<222>117
<223>Tyr或Phe
<221>变体
<222>119
<223>Arg或Lys
<221>变体
<222>128
<223>Glu或Asp
<221>变体
<222>129
<223>Val或Ile
<221>变体
<222>131
<223>Asp或Glu
<221>变体
<222>135
<223>Val或Ala
<221>变体
<222>139
<223>Ile或Val
<221>变体
<222>140
<223>Leu或Met
<221>变体
<222>145
<223>Ile或Leu
<400>953
Met Ile Glu Xaa Lys Pro Ile Asn Ala Glu Asp Thr Tyr Xaa Leu Arg
1 5 10 15
His Xaa Xaa Leu Arg Pro Asn Gln Pro Xaa Glu Xaa Cys Xaa Xaa Xaa
20 25 30
Xaa Asp Leu Xaa Arg Xaa Ala Phe His Leu Gly Gly Xaa Tyr Xaa Gly
35 40 45
Xaa Leu Xaa Ser Xaa Ala Ser Phe His Xaa Ala Xaa His Xaa Xaa Xaa
50 55 60
Gln Gly Xaa Lys Gln Tyr Gln Xaa Arg Gly Xaa Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Xaa Gln Lys Ala Gly Xaa Xaa Leu Xaa Xaa His Ala Glu Glu
85 90 95
Ile Xaa Arg Lys Arg Gly Xaa Asp Xaa Xaa Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Xaa Tyr Xaa Lys Leu Gly Phe Ser Glu Gln Gly Xaa
115 120 125
Xaa Phe Xaa Thr Pro Pro Xaa Gly Pro His Xaa Xaa Met Tyr Lys Arg
130 135 140
Xaa Thr
145
<210>954
<211>146
<212>PRT
<213>人工序列
<220>
<223>con alt a
<221>变体
<222>14
<223>Asp或Glu
<221>变体
<222>32
<223>Glu或Asp
<221>变体
<222>33
<223>Ser或Asn
<221>变体
<222>38
<223>Gly或Ser
<221>变体
<222>46
<223>His或Tyr
<221>变体
<222>51
<223>Ile或Val
<221>变体
<222>58
<223>Gln或Arg
<221>变体
<222>60
<223>Glu或Gly
<221>变体
<222>62
<223>Ser或Thr
<221>变体
<222>67
<223>Gln或Lys
<221>变体
<222>88
<223>Ser或Thr
<221>变体
<222>103
<223>Ala或Val
<221>变体
<222>105
<223>Met或Leu
<221>变体
<222>106
<223>Ile或Leu
<221>变体
<222>119
<223>Arg或Lys
<221>变体
<222>139
<223>Ile或Val
<400>954
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Xaa Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Xaa
20 25 30
Xaa Asp Leu Met Arg Xaa Ala Phe His Leu Gly Gly Phe Xaa Gly Gly
35 40 45
Lys Leu Xaa Ser Val Ala Ser Phe His Xaa Ala Xaa His Xaa Glu Leu
50 55 60
Gln Gly Xaa Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Xaa Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Xaa Asp Xaa Xaa Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Xaa Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Xaa Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>955
<211>146
<212>PRT
<213>人工序列
<220>
<223>con alt b
<221>变体
<222>14
<223>Asp或Glu
<221>变体
<222>32
<223>Glu或Asp
<221>变体
<222>33
<223>Ser或Asn
<221>变体
<222>38
<223>Gly或Ser
<221>变体
<222>46
<223>His或Tyr
<221>变体
<222>51
<223>Ile或Val
<221>变体
<222>58
<223>Gln或Arg
<221>变体
<222>60
<223>Glu或Gly
<221>变体
<222>62
<223>Ser或Thr
<221>变体
<222>67
<223>Gln或Lys
<221>变体
<222>88
<223>Ser或Thr
<221>变体
<222>103
<223>Ala或Val
<221>变体
<222>106
<223>Ile或Leu
<221>变体
<222>119
<223>Arg或Lys
<221>变体
<222>139
<223>Ile或Val
<400>955
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Xaa Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Xaa
20 25 30
Xaa Asp Leu Thr Arg Xaa Ala Phe His Leu Gly Gly Phe Xaa Gly Gly
35 40 45
Lys Leu Xaa Ser Val Ala Ser Phe His Xaa Ala Xaa His Xaa Glu Leu
50 55 60
Gln Gly Xaa Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Xaa Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Xaa Asp Met Xaa Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Xaa Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Xaa Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>956
<211>146
<212>PRT
<213>人工序列
<220>
<223>con alt c
<400>956
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Glu
20 25 30
Ser Asp Leu Thr Arg Gly Ala Phe His Leu Gly Gly Phe Tyr Gly Gly
35 40 45
Lys Leu Ile Ser Val Ala Ser Phe His Gln Ala Glu His Ser Glu Leu
50 55 60
Gln Gly Gln Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Ala Asp Met Leu Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Ile Leu MetTyr Lys Arg
130 135 140
Ile Thr
145
<210>957
<211>146
<212>PRT
<213>人工序列
<220>
<223>con alt e
<221>变体
<222>14
<223>Asp或Glu
<221>变体
<222>33
<223>Gly或Ser或Asn
<221>变体
<222>38
<223>Gly或Ser
<221>变体
<222>46
<223>His或Tyr
<221>变体
<222>51
<223>Ile或Val
<221>变体
<222>58
<223>Gln或Arg
<221>变体
<222>60
<223>Glu或Gly
<221>变体
<222>62
<223>Ser或Thr
<221>变体
<222>67
<223>Gln或Lys或Arg
<221>变体
<222>88
<223>Ser或Thr
<221>变体
<222>103
<223>Ala或Val
<221>变体
<222>105
<223>Leu或Met
<221>变体
<222>106
<223>Ile或Leu
<221>变体
<222>119
<223>Arg或Lys
<221>变体
<222>139
<223>Ile或Val
<400>957
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Xaa Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Xaa Asp Leu Thr Arg Xaa Ala Phe His Leu Gly Gly Phe Xaa Gly Gly
35 40 45
Lys Leu Xaa Ser Val Ala Ser Phe His Xaa Ala Xaa His Xaa Glu Leu
50 55 60
Gln Gly Xaa Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Xaa Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Xaa Asp Xaa Xaa Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Xaa Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Xaa Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>958
<211>146
<212>PRT
<213>人工序列
<220>
<223>con alt f
<221>变体
<222>32
<223>Glu或Asp
<221>变体
<222>33
<223>Gly或Ser或Asn
<221>变体
<222>36
<223>Thr或Met
<221>变体
<222>38
<223>Gly或Ser
<221>变体
<222>46
<223>His或Tyr
<221>变体
<222>51
<223>Ile或Val
<221>变体
<222>58
<223>Gln或Arg
<221>变体
<222>60
<223>Glu或Gly
<221>变体
<222>62
<223>Ser或Thr
<221>变体
<222>67
<223>Gln或Lys或Arg
<221>变体
<222>88
<223>Ser或Thr
<221>变体
<222>103
<223>Ala或Val
<221>变体
<222>106
<223>Ile或Leu
<221>变体
<222>119
<223>Arg或Lys
<221>变体
<222>139
<223>Ile或Val
<400>958
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Xaa
20 25 30
Xaa Asp Leu Xaa Arg Xaa Ala Phe His Leu Gly Gly Phe Xaa Gly Gly
35 40 45
Lys Leu Xaa Ser Val Ala Ser Phe His Xaa Ala Xaa His Xaa Glu Leu
50 55 60
Gln Gly Xaa Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Xaa Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Xaa Asp Leu Xaa Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Xaa Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Xaa Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>959
<211>146
<212>PRT
<213>人工序列
<220>
<223>con alt g
<221>变体
<222>33
<223>Gly或Ser或Asn
<221>变体
<222>38
<223>Gly或Ser
<221>变体
<222>46
<223>His或Tyr
<221>变体
<222>51
<223>Ile或Val
<221>变体
<222>58
<223>Gln或Arg
<221>变体
<222>60
<223>Glu或Gly
<221>变体
<222>62
<223>Ser或Thr
<221>变体
<222>67
<223>Gln或Lys或Arg
<221>变体
<222>88
<223>Ser或Thr
<221>变体
<222>103
<223>Ala或Val
<221>变体
<222>105
<223>Leu或Met
<221>变体
<222>106
<223>Ile或Leu
<221>变体
<222>119
<223>Arg或Lys
<221>变体
<222>139
<223>Ile或Val
<400>959
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Glu Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Xaa Asp Leu Met Arg Xaa Ala Phe His Leu Gly Gly Phe Xaa Gly Gly
35 40 45
Lys Leu Xaa Ser Val Ala Ser Phe His Xaa Ala Xaa His Xaa Glu Leu
50 55 60
Gln Gly Xaa Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Xaa Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Xaa Asp Xaa Xaa Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Xaa Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Xaa Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>960
<211>146
<212>PRT
<213>人工序列
<220>
<223>con alt h
<221>变体
<222>14
<223>Asp或Glu
<221>变体
<222>32
<223>Glu或Asp
<221>变体
<222>33
<223>Gly或Ser或Asn
<221>变体
<222>36
<223>Thr或Met
<221>变体
<222>46
<223>His或Tyr
<221>变体
<222>51
<223>Ile或Val
<221>变体
<222>58
<223>Gln或Arg
<221>变体
<222>60
<223>Glu或Gly
<221>变体
<222>62
<223>Ser或Thr
<221>变体
<222>67
<223>Gln或Lys或Arg
<221>变体
<222>88
<223>Ser或Thr
<221>变体
<222>103
<223>Ala或Val
<221>变体
<222>105
<223>Leu或Met
<221>变体
<222>106
<223>Ile或Leu
<221>变体
<222>119
<223>Arg或Lys
<221>变体
<222>139
<223>Ile或Val
<400>960
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Xaa Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Xaa
20 25 30
Xaa Asp Leu Xaa Arg Ser Ala Phe His Leu Gly Gly Phe Xaa Gly Gly
35 40 45
Lys Leu Xaa Ser Val Ala Ser Phe His Xaa Ala Xaa His Xaa Glu Leu
50 55 60
Gln Gly Xaa Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Xaa Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Xaa Asp Xaa Xaa Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Xaa Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Xaa Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>961
<211>146
<212>PRT
<213>人工序列
<220>
<223>con alt i
<221>变体
<222>14
<223>Asp或Glu
<221>变体
<222>36
<223>Thr或Met
<221>变体
<222>38
<223>Gly或Ser
<221>变体
<222>46
<223>His或Tyr
<221>变体
<222>51
<223>Ile或Val
<221>变体
<222>58
<223>Gln或Arg
<221>变体
<222>60
<223>Glu或Gly
<221>变体
<222>62
<223>Ser或Thr
<221>变体
<222>67
<223>Gln或Lys或Arg
<221>变体
<222>88
<223>Ser或Thr
<221>变体
<222>103
<223>Ala或Val
<221>变体
<222>105
<223>Leu或Met
<221>变体
<222>106
<223>Ile或Leu
<221>变体
<222>119
<223>Arg或Lys
<221>变体
<222>139
<223>Ile或Val
<400>961
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Xaa Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Asp
20 25 30
Asn Asp Leu Xaa Arg Xaa Ala Phe His Leu Gly Gly Phe Xaa Gly Gly
35 40 45
Lys Leu Xaa Ser Val Ala Ser Phe His Xaa Ala Xaa His Xaa Glu Leu
50 55 60
Gln Gly Xaa Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Xaa Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Xaa Asp Xaa Xaa Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Xaa Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Xaa Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>962
<211>146
<212>PRT
<213>人工序列
<220>
<223>con alt j
<221>变体
<222>14
<223>Asp或Glu
<221>变体
<222>32
<223>Glu或Asp
<221>变体
<222>33
<223>Gly或Ser或Asn
<221>变体
<222>36
<223>Thr或Met
<221>变体
<222>38
<223>Gly或Ser
<221>变体
<222>46
<223>His或Tyr
<221>变体
<222>51
<223>Ile或Val
<221>变体
<222>58
<223>Gln或Arg
<221>变体
<222>60
<223>Glu或Gly
<221>变体
<222>62
<223>Ser或Thr
<221>变体
<222>67
<223>Gln或Lys或Arg
<221>变体
<222>88
<223>Ser或Thr
<221>变体
<222>103
<223>Ala或Val
<221>变体
<222>106
<223>Ile或Leu
<221>变体
<222>119
<223>Arg或Lys
<221>变体
<222>139
<223>Ile或Val
<400>962
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Xaa Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Xaa
20 25 30
Xaa Asp Leu Xaa Arg Xaa Ala Phe His Leu Gly Gly Phe Xaa Gly Gly
35 40 45
Lys Leu Xaa Ser Val Ala Ser Phe His Xaa Ala Xaa His Xaa Glu Leu
50 55 60
Gln Gly Xaa Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Glu Gln Lys Ala Gly Xaa Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Xaa Asp Met Xaa Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Xaa Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Xaa Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>963
<211>146
<212>PRT
<213>人工序列
<220>
<223>con alt k
<221>变体
<222>4
<223>Val或Ile
<221>变体
<222>18
<223>Arg或Lys
<221>变体
<222>26
<223>Ile或Leu或Val
<221>变体
<222>28
<223>Ala或Val
<221>变体
<222>30
<223>Met或Ile
<221>变体
<222>31
<223>Phe或Tyr
<221>变体
<222>32
<223>Glu或Asp
<221>变体
<222>33
<223>Ser或Asn
<221>变体
<222>38
<223>Gly或Ser
<221>变体
<222>45
<223>Phe或Tyr
<221>变体
<222>47
<223>Gly或Arg
<221>变体
<222>49
<223>Lys或Arg
<221>变体
<222>51
<223>Ile或Val
<221>变体
<222>53
<223>Val或Ile
<221>变体
<222>58
<223>Gln或Arg
<221>变体
<222>60
<223>Glu或Gly
<221>变体
<222>62
<223>Ser或Thr
<221>变体
<222>63
<223>Glu或Asp
<221>变体
<222>64
<223>Leu或Ile
<221>变体
<222>67
<223>Gln或Lys或Arg
<221>变体
<222>72
<223>Leu或Ile
<221>变体
<222>75
<223>Val或Met
<221>变体
<222>83
<223>Asp或Glu
<221>变体
<222>88
<223>Ser或Thr
<221>变体
<222>89
<223>Ser或Thr
<221>变体
<222>91
<223>Val或Ile或Leu
<221>变体
<222>92
<223>Lys或Arg
<221>变体
<222>98
<223>Leu或Ile
<221>变体
<222>103
<223>Ala或Val
<221>变体
<222>105
<223>Met或Leu或Ile
<221>变体
<222>106
<223>Ile或Leu
<221>变体
<222>117
<223>Tyr或Phe
<221>变体
<222>119
<223>Arg或Lys
<221>变体
<222>128
<223>Glu或Asp
<221>变体
<222>129
<223>Val或Ile
<221>变体
<222>131
<223>Asp或Glu
<221>变体
<222>135
<223>Val或Ala
<221>变体
<222>139
<223>Ile或Val
<221>变体
<222>140
<223>Leu或Met
<221>变体
<222>145
<223>Ile或Leu
<400>963
Met Ile Glu Xaa Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Xaa Val Leu Arg Pro Asn Gln Pro Xaa Glu Xaa Cys Xaa Xaa Xaa
20 25 30
Xaa Asp Leu Met Arg Xaa Ala Phe His Leu Gly Gly Xaa Tyr Xaa Gly
35 40 45
Xaa Leu Xaa Ser Xaa Ala Ser Phe His Xaa Ala Xaa His Xaa Xaa Xaa
50 55 60
Gln Gly Xaa Lys Gln Tyr Gln Xaa Arg Gly Xaa Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Xaa Gln Lys Ala Gly Xaa Xaa Leu Xaa Xaa His Ala Glu Glu
85 90 95
Ile Xaa Arg Lys Arg Gly Xaa Asp Xaa Xaa Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Xaa Tyr Xaa Lys Leu Gly Phe Ser Glu Gln Gly Xaa
115 120 125
Xaa Phe Xaa Thr Pro Pro Xaa Gly Pro His Xaa Xaa Met Tyr Lys Arg
130 135 140
Xaa Thr
145
<210>964
<211>146
<212>PRT
<213>人工序列
<220>
<223>con alt l
<221>变体
<222>4
<223>Val或Ile
<221>变体
<222>14
<223>Asp或Glu
<221>变体
<222>18
<223>Arg或Lys
<221>变体
<222>26
<223>Ile或Leu或Val
<221>变体
<222>28
<223>Ala或Val
<221>变体
<222>30
<223>Met或Ile
<221>变体
<222>31
<223>Phe或Tyr
<221>变体
<222>32
<223>Glu或Asp
<221>变体
<222>33
<223>Ser或Asn
<221>变体
<222>38
<223>Gly或Ser
<221>变体
<222>45
<223>Phe或Tyr
<221>变体
<222>47
<223>Gly或Arg
<221>变体
<222>49
<223>Lys或Arg
<221>变体
<222>51
<223>Ile或Val
<221>变体
<222>58
<223>Gln或Arg
<221>变体
<222>60
<223>Glu或Gly
<221>变体
<222>62
<223>Ser或Thr
<221>变体
<222>63
<223>Glu或Asp
<221>变体
<222>64
<223>Leu或Ile
<221>变体
<222>67
<223>Gln或Lys或Arg
<221>变体
<222>72
<223>Leu或Ile
<221>变体
<222>75
<223>Val或Met
<221>变体
<222>83
<223>Asp或Glu
<221>变体
<222>88
<223>Ser或Thr
<221>变体
<222>89
<223>Ser或Thr
<221>变体
<222>91
<223>Val或Ile或Leu
<221>变体
<222>92
<223>Lys或Arg
<221>变体
<222>98
<223>Leu或Ile
<221>变体
<222>103
<223>Ala或Val
<221>变体
<222>105
<223>Met或Leu或Ile
<221>变体
<222>106
<223>Ile或Leu
<221>变体
<222>117
<223>Tyr或Phe
<221>变体
<222>119
<223>Arg或Lys
<221>变体
<222>128
<223>Glu或Asp
<221>变体
<222>129
<223>Val或Ile
<221>变体
<222>131
<223>Asp或Glu
<221>变体
<222>135
<223>Val或Ala
<221>变体
<222>139
<223>Ile或Val
<221>变体
<222>140
<223>Leu或Met
<221>变体
<222>145
<223>Ile或Leu
<400>964
Met Ile Glu Xaa Lys Pro Ile Asn Ala Glu Asp Thr Tyr Xaa Leu Arg
1 5 10 15
His Xaa Val Leu Arg Pro Asn Gln Pro Xaa Glu Xaa Cys Xaa Xaa Xaa
20 25 30
Xaa Asp Leu Met Arg Xaa Ala Phe His Leu Gly Gly Xaa Tyr Xaa Gly
35 40 45
Xaa Leu Xaa Ser Val Ala Ser Phe His Xaa Ala Xaa His Xaa Xaa Xaa
50 55 60
Gln Gly Xaa Lys Gln Tyr Gln Xaa Arg Gly Xaa Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Xaa Gln Lys Ala Gly Xaa Xaa Leu Xaa Xaa His Ala Glu Glu
85 90 95
Ile Xaa Arg Lys Arg Gly Xaa Asp Xaa Xaa Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Xaa Tyr Xaa Lys Leu Gly Phe Ser Glu Gln Gly Xaa
115 120 125
Xaa Phe Xaa Thr Pro Pro Xaa Gly Pro His Xaa Xaa Met Tyr Lys Arg
130 135 140
Xaa Thr
145
<210>965
<211>146
<212>PRT
<213>人工序列
<220>
<223>con alt d
<221>变体
<222>14
<223>Asp或Glu
<221>变体
<222>32
<223>Glu或Asp
<221>变体
<222>33
<223>Ser或Asn或Gly
<221>变体
<222>36
<223>Thr或Met
<221>变体
<222>46
<223>His或Tyr
<221>变体
<222>51
<223>Ile或Val
<221>变体
<222>58
<223>Gln或Arg
<221>变体
<222>60
<223>Glu或Gly
<221>变体
<222>67
<223>Gln或Lys或Arg
<221>变体
<222>103
<223>Ala或Val
<221>变体
<222>105
<223>Met或Leu
<221>变体
<222>106
<223>Ile或Leu
<221>变体
<222>119
<223>Arg或Lys
<221>变体
<222>139
<223>Ile或Val
<400>965
Met Ile Glu Val Lys Pro Ile Asn Ala Glu Asp Thr Tyr Xaa Leu Arg
1 5 10 15
His Arg Val Leu Arg Pro Asn Gln Pro Ile Glu Ala Cys Met Phe Xaa
20 25 30
Xaa Asp Leu Xaa Arg Xaa Ala Phe His Leu Gly Gly Phe Xaa Gly Gly
35 40 45
Lys Leu Xaa Ser Val Ala Ser Phe His Xaa Ala Xaa His Thr Glu Leu
50 55 60
Gln Gly Xaa Lys Gln Tyr Gln Leu Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Gln Gln Lys Ala Gly Ser Ser Leu Val Lys His Ala Glu Glu
85 90 95
Ile Leu Arg Lys Arg Gly Xaa Asp Xaa Xaa Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Tyr Tyr Xaa Lys Leu Gly Phe Ser Glu Gln Gly Glu
115 120 125
Val Phe Asp Thr Pro Pro Val Gly Pro His Xaa Leu Met Tyr Lys Arg
130 135 140
Ile Thr
145
<210>966
<211>146
<212>PRT
<213>人工序列
<220>
<223>con alt n
<221>变体
<222>4
<223>Val或Ile
<221>变体
<222>14
<223>Asp或Glu
<221>变体
<222>18
<223>Arg或Lys
<221>变体
<222>19
<223>Val或Ile
<221>变体
<222>26
<223>Ile或Leu或Val
<221>变体
<222>28
<223>Ala或Val
<221>变体
<222>30
<223>Met或Ile
<221>变体
<222>31
<223>Phe或Tyr
<221>变体
<222>32
<223>Glu或Asp
<221>变体
<222>33
<223>Ser或Asn
<221>变体
<222>36
<223>Thr或Met或Leu
<221>变体
<222>38
<223>Gly或Ser
<221>变体
<222>45
<223>Phe或Tyr
<221>变体
<222>47
<223>Gly或Arg
<221>变体
<222>49
<223>Lys或Arg
<221>变体
<222>51
<223>Ile或Val
<221>变体
<222>53
<223>Val或Ile
<221>变体
<222>58
<223>Gln或Arg
<221>变体
<222>60
<223>Glu或Gly
<221>变体
<222>62
<223>Ser或Thr
<221>变体
<222>63
<223>Glu或Asp
<221>变体
<222>64
<223>Leu或Ile
<221>变体
<222>67
<223>Gln或Lys或Arg
<221>变体
<222>72
<223>Leu或Ile
<221>变体
<222>75
<223>Val或Met
<221>变体
<222>83
<223>Asp或Glu
<221>变体
<222>89
<223>Ser或Thr
<221>变体
<222>91
<223>Val或Ile或Leu
<221>变体
<222>92
<223>Lys或Arg
<221>变体
<222>98
<223>Leu或Ile
<221>变体
<222>103
<223>Ala或Val
<221>变体
<222>105
<223>Met或Leu或Ile
<221>变体
<222>106
<223>Ile或Leu
<221>变体
<222>117
<223>Tyr或Phe
<221>变体
<222>119
<223>Arg或Lys
<221>变体
<222>128
<223>Glu或Asp
<221>变体
<222>129
<223>Val或Ile
<221>变体
<222>131
<223>Asp或Glu
<221>变体
<222>135
<223>Val或Ala
<221>变体
<222>139
<223>Ile或Val
<221>变体
<222>140
<223>Leu或Met
<221>变体
<222>145
<223>Ile或Leu
<400>966
Met Ile Glu Xaa Lys Pro Ile Asn Ala Glu Asp Thr Tyr Xaa Leu Arg
1 5 10 15
His Xaa Xaa Leu Arg Pro Asn Gln Pro Xaa Glu Xaa Cys Xaa Xaa Xaa
20 25 30
Xaa Asp Leu Xaa Arg Xaa Ala Phe His Leu Gly Gly Xaa Tyr Xaa Gly
35 40 45
Xaa Leu Xaa Ser Xaa Ala Ser Phe His Xaa Ala Xaa His Xaa Xaa Xaa
50 55 60
Gln Gly Xaa Lys Gln Tyr Gln Xaa Arg Gly Xaa Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Xaa Gln Lys Ala Gly Thr Xaa Leu Xaa Xaa His Ala Glu Glu
85 90 95
Ile Xaa Arg Lys Arg Gly Xaa Asp Xaa Xaa Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Xaa Tyr Xaa Lys Leu Gly Phe Ser Glu Gln Gly Xaa
115 120 125
Xaa Phe Xaa Thr Pro Pro Xaa Gly Pro His Xaa Xaa Met Tyr Lys Arg
130 135 140
Xaa Thr
145
<210>967
<211>146
<212>PRT
<213>人工序列
<220>
<223>con alt o
<221>变体
<222>4
<223>Val或Ile
<221>变体
<222>14
<223>Asp或Glu
<221>变体
<222>18
<223>Arg或Lys
<221>变体
<222>19
<223>Val或Ile
<221>变体
<222>26
<223>Ile或Leu或Val
<221>变体
<222>28
<223>Ala或Val
<221>变体
<222>30
<223>Met或Ile
<221>变体
<222>31
<223>Phe或Tyr
<221>变体
<222>32
<223>Glu或Asp
<221>变体
<222>33
<223>Ser或Asn
<221>变体
<222>36
<223>Thr或Met或Leu
<221>变体
<222>38
<223>Gly或Ser
<221>变体
<222>45
<223>Phe或Tyr
<221>变体
<222>47
<223>Gly或Arg
<221>变体
<222>49
<223>Lys或Arg
<221>变体
<222>51
<223>Ile或Val
<221>变体
<222>53
<223>Val或Ile
<221>变体
<222>58
<223>Gln或Arg
<221>变体
<222>60
<223>Glu或Gly
<221>变体
<222>62
<223>Ser或Thr
<221>变体
<222>63
<223>Glu或Asp
<221>变体
<222>64
<223>Leu或Ile
<221>变体
<222>67
<223>Gln或Lys或Arg
<221>变体
<222>72
<223>Leu或Ile
<221>变体
<222>75
<223>Val或Met
<221>变体
<222>83
<223>Asp或Glu
<221>变体
<222>88
<223>Ser或Thr
<221>变体
<222>89
<223>Ser或Thr
<221>变体
<222>91
<223>Val或Ile或Leu
<221>变体
<222>92
<223>Lys或Arg
<221>变体
<222>98
<223>Leu或Ile
<221>变体
<222>103
<223>Ala或Val
<221>变体
<222>105
<223>Met或Leu或Ile
<221>变体
<222>106
<223>Ile或Leu
<221>变体
<222>117
<223>Tyr或Phe
<221>变体
<222>128
<223>Glu或Asp
<221>变体
<222>129
<223>Val或Ile
<221>变体
<222>135
<223>Val或Ala
<221>变体
<222>139
<223>Ile或Val
<221>变体
<222>140
<223>Leu或Met
<221>变体
<222>145
<223>Ile或Leu
<400>967
Met Ile Glu Xaa Lys Pro Ile Asn Ala Glu Asp Thr Tyr Xaa Leu Arg
1 5 10 15
His Xaa Xaa Leu Arg Pro Asn Gln Pro Xaa Glu Xaa Cys Xaa Xaa Xaa
20 25 30
Xaa Asp Leu Xaa Arg Xaa Ala Phe His Leu Gly Gly Xaa Tyr Xaa Gly
35 40 45
Xaa Leu Xaa Ser Xaa Ala Ser Phe His Xaa Ala Xaa His Xaa Xaa Xaa
50 55 60
Gln Gly Xaa Lys Gln Tyr Gln Xaa Arg Gly Xaa Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Xaa Gln Lys Ala Gly Xaa Xaa Leu Xaa Xaa His Ala Glu Glu
85 90 95
Ile Xaa Arg Lys Arg Gly Xaa Asp Xaa Xaa Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Xaa Tyr Arg Lys Leu Gly Phe Ser Glu Gln Gly Xaa
115 120 125
Xaa Phe Asp Thr Pro Pro Xaa Gly Pro His Xaa Xaa Met Tyr Lys Arg
130 135 140
Xaa Thr
145
<210>968
<211>146
<212>PRT
<213>人工序列
<220>
<223>con alt p
<221>变体
<222>4
<223>Val或Ile
<221>变体
<222>14
<223>Asp或Glu
<221>变体
<222>18
<223>Arg或Lys
<221>变体
<222>26
<223>Ile或Leu或Val
<221>变体
<222>28
<223>Ala或Val
<221>变体
<222>30
<223>Met或Ile
<221>变体
<222>31
<223>Phe或Tyr
<221>变体
<222>32
<223>Glu或Asp
<221>变体
<222>33
<223>Ser或Asn
<221>变体
<222>36
<223>Thr或Met或Leu
<221>变体
<222>38
<223>Gly或Ser
<221>变体
<222>45
<223>Phe或Tyr
<221>变体
<222>47
<223>Gly或Arg
<221>变体
<222>49
<223>Lys或Arg
<221>变体
<222>51
<223>Ile或Val
<221>变体
<222>53
<223>Val或Ile
<221>变体
<222>58
<223>Gln或Arg
<221>变体
<222>60
<223>Glu或Gly
<221>变体
<222>62
<223>Ser或Thr
<221>变体
<222>63
<223>Glu或Asp
<221>变体
<222>64
<223>Leu或Ile
<221>变体
<222>67
<223>Gln或Lys或Arg
<221>变体
<222>72
<223>Leu或Ile
<221>变体
<222>83
<223>Asp或Glu
<221>变体
<222>88
<223>Ser或Thr
<221>变体
<222>89
<223>Ser或Thr
<221>变体
<222>91
<223>Val或Ile或Leu
<221>变体
<222>92
<223>Lys或Arg
<221>变体
<222>98
<223>Leu或Ile
<221>变体
<222>103
<223>Ala或Val
<221>变体
<222>105
<223>Met或Leu或Ile
<221>变体
<222>106
<223>Ile或Leu
<221>变体
<222>117
<223>Tyr或Phe
<221>变体
<222>119
<223>Arg或Lys
<221>变体
<222>128
<223>Glu或Asp
<221>变体
<222>129
<223>Val或Ile
<221>变体
<222>131
<223>Asp或Glu
<221>变体
<222>135
<223>Val或Ala
<221>变体
<222>139
<223>Ile或Val
<221>变体
<222>140
<223>Leu或Met
<221>变体
<222>145
<223>Ile或Leu
<400>968
Met Ile Glu Xaa Lys Pro Ile Asn Ala Glu Asp Thr Tyr Xaa Leu Arg
1 5 10 15
His Xaa Val Leu Arg Pro Asn Gln Pro Xaa Glu Xaa Cys Xaa Xaa Xaa
20 25 30
Xaa Asp Leu Xaa Arg Xaa Ala Phe His Leu Gly Gly Xaa Tyr Xaa Gly
35 40 45
Xaa Leu Xaa Ser Xaa Ala Ser Phe His Xaa Ala Xaa His Xaa Xaa Xaa
50 55 60
Gln Gly Xaa Lys Gln Tyr Gln Xaa Arg Gly Val Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Xaa Gln Lys Ala Gly Xaa Xaa Leu Xaa Xaa His Ala Glu Glu
85 90 95
Ile Xaa Arg Lys Arg Gly Xaa Asp Xaa Xaa Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Xaa Tyr Xaa Lys Leu Gly Phe Ser Glu Gln Gly Xaa
115 120 125
Xaa Phe Xaa Thr Pro Pro Xaa Gly Pro His Xaa Xaa Met Tyr Lys Arg
130 135 140
Xaa Thr
145
<210>969
<211>146
<212>PRT
<213>人工序列
<220>
<223>con alt q
<221>变体
<222>4
<223>Val或Ile
<221>变体
<222>14
<223>Asp或Glu
<221>变体
<222>18
<223>Arg或Lys
<221>变体
<222>19
<223>Val或Ile
<221>变体
<222>26
<223>Ile或Leu或Val
<221>变体
<222>28
<223>Ala或Val
<221>变体
<222>30
<223>Met或Ile
<221>变体
<222>31
<223>Phe或Tyr
<221>变体
<222>32
<223>Glu或Asp
<221>变体
<222>33
<223>Ser或Asn
<221>变体
<222>36
<223>Thr或Met或Leu
<221>变体
<222>38
<223>Gly或Ser
<221>变体
<222>45
<223>Phe或Tyr
<221>变体
<222>47
<223>Gly或Arg
<221>变体
<222>51
<223>Ile或Val
<221>变体
<222>53
<223>Val或Ile
<221>变体
<222>58
<223>Gln或Arg
<221>变体
<222>60
<223>Glu或Gly
<221>变体
<222>62
<223>Ser或Thr
<221>变体
<222>63
<223>Glu或Asp
<221>变体
<222>64
<223>Leu或Ile
<221>变体
<222>67
<223>Gln或Lys或Arg
<221>变体
<222>72
<223>Leu或Ile
<221>变体
<222>75
<223>Val或Met
<221>变体
<222>83
<223>Asp或Glu
<221>变体
<222>88
<223>Ser或Thr
<221>变体
<222>89
<223>Ser或Thr
<221>变体
<222>91
<223>Val或Ile或Leu
<221>变体
<222>92
<223>Lys或Arg
<221>变体
<222>98
<223>Leu或Ile
<221>变体
<222>103
<223>Ala或Val
<221>变体
<222>105
<223>Met或Leu或Ile
<221>变体
<222>106
<223>Ile或Leu
<221>变体
<222>117
<223>Tyr或Phe
<221>变体
<222>119
<223>Arg或Lys
<221>变体
<222>128
<223>Glu或Asp
<221>变体
<222>129
<223>Val或Ile
<221>变体
<222>131
<223>Asp或Glu
<221>变体
<222>135
<223>Val或Ala
<221>变体
<222>139
<223>Ile或Val
<221>变体
<222>140
<223>Leu或Met
<221>变体
<222>145
<223>Ile Leu
<400>969
Met Ile Glu Xaa Lys Pro Ile Asn Ala Glu Asp Thr Tyr Xaa Leu Arg
l 5 10 15
His Xaa Xaa Leu Arg Pro Asn Gln Pro Xaa Glu Xaa Cys Xaa Xaa Xaa
20 25 30
Xaa Asp Leu Xaa Arg Xaa Ala Phe His Leu Gly Gly Xaa Tyr Xaa Gly
35 40 45
Arg Leu Xaa Ser Xaa Ala Ser Phe His Xaa Ala Xaa His Xaa Xaa Xaa
50 55 60
Gln Gly Xaa Lys Gln Tyr Gln Xaa Arg Gly Xaa Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Xaa Gln Lys Ala Gly Xaa Xaa Leu Xaa Xaa His Ala Glu Glu
85 90 95
Ile Xaa Arg Lys Arg Gly Xaa Asp Xaa Xaa Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Xaa Tyr Xaa Lys Leu Gly Phe Ser Glu Gln Gly Xaa
115 120 125
Xaa Phe Xaa Thr Pro Pro Xaa Gly Pro His Xaa Xaa Met Tyr Lys Arg
130 135 140
Xaa Thr
145
<210>970
<211>146
<212>PRT
<213>人工序列
<220>
<223>con alt r
<221>变体
<222>4
<223>Val或Ile
<221>变体
<222>18
<223>Arg或Lys
<221>变体
<222>19
<223>Val或Ile
<221>变体
<222>26
<223>Ile或Leu或Val
<221>变体
<222>28
<223>Ala或Val
<221>变体
<222>30
<223>Met或Ile
<221>变体
<222>31
<223>Phe或Tyr
<221>变体
<222>32
<223>Glu或Asp
<221>变体
<222>33
<223>Ser或Asn
<221>变体
<222>36
<223>Thr或Met或Leu
<221>变体
<222>38
<223>Gly或Ser
<221>变体
<222>45
<223>Phe或Tyr
<221>变体
<222>47
<223>Gly或Arg
<221>变体
<222>49
<223>Lys或Arg
<221>变体
<222>51
<223>Ile或Val
<221>变体
<222>58
<223>Gln或Arg
<221>变体
<222>60
<223>Glu或Gly
<221>变体
<222>62
<223>Ser或Thr
<221>变体
<222>63
<223>Glu或Asp
<221>变体
<222>64
<223>Leu或Ile
<221>变体
<222>67
<223>Gln或Lys或Arg
<221>变体
<222>72
<223>Leu或Ile
<221>变体
<222>75
<223>Val或Met
<221>变体
<222>83
<223>Asp或Glu
<221>变体
<222>88
<223>Ser或Thr
<221>变体
<222>89
<223>Ser或Thr
<221>变体
<222>91
<223>Val或Ile或Leu
<221>变体
<222>92
<223>Lys或Arg
<221>变体
<222>98
<223>Leu或Ile
<221>变体
<222>103
<223>Ala或Val
<221>变体
<222>105
<223>Met或Leu或Ile
<221>变体
<222>106
<223>Ile或Leu
<221>变体
<222>117
<223>Tyr或Phe
<221>变体
<222>119
<223>Arg或Lys
<221>变体
<222>128
<223>Glu或Asp
<221>变体
<222>129
<223>Val或Ile
<221>变体
<222>131
<223>Asp或Glu
<221>变体
<222>135
<223>Val或Ala
<221>变体
<222>139
<223>Ile或Val
<221>变体
<222>140
<223>Leu或Met
<221>变体
<222>145
<223>Ile或Leu
<400>970
Met Ile Glu Xaa Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Xaa Xaa Leu Arg Pro Asn Gln Pro Xaa Glu Xaa Cys Xaa Xaa Xaa
20 25 30
Xaa Asp Leu Xaa Arg Xaa Ala Phe His Leu Gly Gly Xaa Tyr Xaa Gly
35 40 45
Xaa Leu Xaa Ser Val Ala Ser Phe His Xaa Ala Xaa His Xaa Xaa Xaa
50 55 60
Gln Gly Xaa Lys Gln Tyr Gln Xaa Arg Gly Xaa Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Xaa Gln Lys Ala Gly Xaa Xaa Leu Xaa Xaa His Ala Glu Glu
85 90 95
Ile Xaa Arg Lys Arg Gly Xaa Asp Xaa Xaa Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Xaa Tyr Xaa Lys Leu Gly Phe Ser Glu Gln Gly Xaa
115 120 125
Xaa Phe Xaa Thr Pro Pro Xaa Gly Pro His Xaa Xaa Met Tyr Lys Arg
130 135 140
Xaa Thr
145
<210>971
<211>146
<212>PRT
<213>人工序列
<220>
<223>con alt s
<221>变体
<222>4
<223>Val或Ile
<221>变体
<222>14
<223>Asp或Glu
<221>变体
<222>18
<223>Arg或Lys
<221>变体
<222>19
<223>Val或Ile
<221>变体
<222>26
<223>Ile或Leu或Val
<221>变体
<222>28
<223>Ala或Val
<221>变体
<222>30
<223>Met或Ile
<221>变体
<222>31
<223>Phe或Tyr
<221>变体
<222>32
<223>Glu或Asp
<221>变体
<222>33
<223>Ser或Asn
<221>变体
<222>38
<223>Gly或Ser
<221>变体
<222>45
<223>Phe或Tyr
<221>变体
<222>47
<223>Gly或Arg
<221>变体
<222>49
<223>Lys或Arg
<221>变体
<222>51
<223>Ile或Val
<221>变体
<222>58
<223>Gln或Arg
<221>变体
<222>60
<223>Glu或Gly
<221>变体
<222>62
<223>Ser或Thr
<221>变体
<222>63
<223>Glu或Asp
<221>变体
<222>64
<223>Leu或Ile
<221>变体
<222>67
<223>Gln或Lys或Arg
<221>变体
<222>72
<223>Leu或Ile
<221>变体
<222>75
<223>Val或Met
<221>变体
<222>83
<223>Asp或Glu
<221>变体
<222>88
<223>Ser或Thr
<221>变体
<222>89
<223>Ser或Thr
<221>变体
<222>91
<223>Val或Ile或Leu
<221>变体
<222>92
<223>Lys或Arg
<221>变体
<222>98
<223>Leu或Ile
<221>变体
<222>103
<223>Ala或Val
<221>变体
<222>105
<223>Met或Leu或Ile
<221>变体
<222>106
<223>Ile或Leu
<221>变体
<222>117
<223>Tyr或Phe
<221>变体
<222>119
<223>Arg或Lys
<221>变体
<222>128
<223>Glu或Asp
<221>变体
<222>129
<223>Val或Ile
<221>变体
<222>131
<223>Asp或Glu
<221>变体
<222>135
<223>Val或Ala
<221>变体
<222>139
<223>Ile或Val
<221>变体
<222>140
<223>Leu或Met
<221>变体
<222>145
<223>Ile或Leu
<400>971
Met Ile Glu Xaa Lys Pro Ile Asn Ala Glu Asp Thr Tyr Xaa Leu Arg
1 5 10 15
His Xaa Xaa Leu Arg Pro Asn Gln Pro Xaa Glu Xaa Cys Xaa Xaa Xaa
20 25 30
Xaa Asp Leu Met Arg Xaa Ala Phe His Leu Gly Gly Xaa Tyr Xaa Gly
35 40 45
Xaa Leu Xaa Ser Val Ala Ser Phe His Xaa Ala Xaa His Xaa Xaa Xaa
50 55 60
Gln Gly Xaa Lys Gln Tyr Gln Xaa Arg Gly Xaa Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Xaa Gln Lys Ala Gly Xaa Xaa Leu Xaa Xaa His Ala Glu Glu
85 90 95
Ile Xaa Arg Lys Arg Gly Xaa Asp Xaa Xaa Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Xaa Tyr Xaa Lys Leu Gly Phe Ser Glu Gln Gly Xaa
115 120 125
Xaa Phe Xaa Thr Pro Pro Xaa Gly Pro His Xaa Xaa Met Tyr Lys Arg
130 135 140
Xaa Thr
145
<210>972
<211>146
<212>PRT
<213>人工序列
<220>
<223>con alt t
<221>变体
<222>4
<223>Val或Ile
<221>变体
<222>18
<223>Arg或Lys
<221>变体
<222>19
<223>Val或Ile
<221>变体
<222>26
<223>Ile或Leu或Val
<221>变体
<222>28
<223>Ala或Val
<221>变体
<222>30
<223>Met或Ile
<221>变体
<222>31
<223>Phe或Tyr
<221>变体
<222>32
<223>Glu或Asp
<221>变体
<222>33
<223>Ser或Asn
<221>变体
<222>36
<223>Thr或Met或Leu
<221>变体
<222>38
<223>Gly或Ser
<221>变体
<222>45
<223>Phe或Tyr
<221>变体
<222>47
<223>Gly或Arg
<221>变体
<222>49
<223>Lys或Arg
<221>变体
<222>51
<223>Ile或Val
<221>变体
<222>53
<223>Val或Ile
<221>变体
<222>58
<223>Gln或Arg
<221>变体
<222>60
<223>Glu或Gly
<221>变体
<222>62
<223>Ser或Thr
<221>变体
<222>63
<223>Glu或Asp
<221>变体
<222>64
<223>Leu或Ile
<221>变体
<222>67
<223>Gln或Lys或Arg
<221>变体
<222>72
<223>Leu或Ile
<221>变体
<222>75
<223>Val或Met
<221>变体
<222>83
<223>Asp或Glu
<221>变体
<222>88
<223>Ser或Thr
<221>变体
<222>89
<223>Ser或Thr
<221>变体
<222>91
<223>Val或Ile或Leu
<221>变体
<222>92
<223>Lys或Arg
<221>变体
<222>98
<223>Leu或Ile
<221>变体
<222>103
<223>Ala或Val
<221>变体
<222>105
<223>Met或Leu或Ile
<221>变体
<222>106
<223>Ile或Leu
<221>变体
<222>117
<223>Tyr或Phe
<221>变体
<222>119
<223>Arg或Lys
<221>变体
<222>128
<223>Glu或Asp
<221>变体
<222>129
<223>Val或Ile
<221>变体
<222>131
<223>Asp或Glu
<221>变体
<222>135
<223>Val或Ala
<221>变体
<222>139
<223>Ile或Val
<221>变体
<222>140
<223>Leu或Met
<221>变体
<222>145
<223>Ile或Leu
<400>972
Met Ile Glu Xaa Lys Pro Ile Asn Ala Glu Asp Thr Tyr Asp Leu Arg
1 5 10 15
His Xaa Xaa Leu Arg Pro Asn Gln Pro Xaa Glu Xaa Cys Xaa Xaa Xaa
20 25 30
Xaa Asp Leu Xaa Arg Xaa Ala Phe His Leu Gly Gly Xaa Tyr Xaa Gly
35 40 45
Xaa Leu Xaa Ser Xaa Ala Ser Phe His Xaa Ala Xaa His Xaa Xaa Xaa
50 55 60
Gln Gly Xaa Lys Gln Tyr Gln Xaa Arg Gly Xaa Ala Thr Leu Glu Gly
65 70 75 80
Tyr Arg Xaa Gln Lys Ala Gly Xaa Xaa Leu Xaa Xaa His Ala Glu Glu
85 90 95
Ile Xaa Arg Lys Arg Gly Xaa Asp Xaa Xaa Trp Cys Asn Ala Arg Thr
100 105 110
Ser Ala Ser Gly Xaa Tyr Xaa Lys Leu Gly Phe Ser Glu Gln Gly Xaa
115 120 125
Xaa Phe Xaa Thr Pro Pro Xaa Gly Pro His Xaa Xaa Met Tyr Lys Arg
130 135 140
Xaa Thr
145
Claims (125)
1.一种分离的或重组的多核苷酸,包含
(a)编码一种氨基酸序列的核苷酸序列,采用BLOSUM62矩阵,存在一个缺口罚11分,延伸一个缺口罚1分,将该氨基酸序列与选自由SEQ ID NO:300、SEQ ID NO:445和SEQ ID NO:457组成的组的序列最佳地比对,产生至少为460的相似性分值,其中该氨基酸序列包含一个或多个满足至少选自由如下组成的组的一种限制的氨基酸残基:
(i)在位置18和38上,为Z5氨基酸残基;
(ii)在位置62上,为Z1氨基酸残基;
(iii)在位置124上,为Z6氨基酸残基;和
(iv)在位置144上,为Z2氨基酸残基,其中:
Z1为选自由A、I、L、M和V组成的组的氨基酸残基;
Z2为选自由F、W和Y组成的组的氨基酸残基;
Z5为选自由D和E组成的组的氨基酸残基;
Z6为选自由C、G和P组成的组的氨基酸残基;或者
(b)其互补的核苷酸序列。
2.权利要求1的分离的或重组的多核苷酸,其中氨基酸序列中对应于如下位置的氨基酸残基至少90%地遵从如下限制:
(a)在位置2、4、15、19、26、28、31、45、51、54、86、90、91、97、103、105、106、114、123、129、139和/或145上,氨基酸残基为B1;和
(b)在位置3、5、8、10、11、14、17、24、27、32、37、47、48、49、52、57、58、61、63、68、69、79、80、82、83、89、92、100、101、104、119、120、125、126、128、131和/或143上,氨基酸残基为B2;
其中B1为选自由A、I、L、M、F、W、Y和V组成的组的氨基酸;而B2是选自由R、N、D、C、Q、E、G、H、K、P、S和T组成的组的氨基酸。
3.权利要求1的分离的或重组的多核苷酸,其中氨基酸序列中对应于如下位置的氨基酸残基至少80%地遵从如下限制:
(a)在位置2、4、15、19、26、28、51、54、86、90、91、97、103、105、106、114、129、139和/或145上,氨基酸残基为Z1;
(b)在位置31和/或45上,氨基酸残基为Z2;
(c)在位置8上,氨基酸残基为Z3;
(d)在位置89上,氨基酸残基为Z3或者Z6;
(e)在位置82、92、101和/或120上,氨基酸残基为Z4;
(f)在位置3、11、27和/或79上,氨基酸残基为Z5;
(g)在位置18上,氨基酸残基为Z4或Z5;
(h)在位置123上,氨基酸残基为Z1或Z2;
(i)在位置12、33、35、39、53、59、112、132、135、140和/或146上,氨基酸残基为Z1或Z3;
(j)在位置30上,氨基酸残基为Z1;
(k)在位置6上,氨基酸残基为Z6;
(l)在位置81上,氨基酸残基为Z2或Z4;
(m)在位置113上,氨基酸残基为Z3;
(n)在位置138上,氨基酸残基为Z4;
(o)在位置142上,氨基酸残基为Z2;
(p)在位置57和/或126上,氨基酸残基为Z3或Z4;
(q)在位置5、17和61上,氨基酸残基为Z4;
(r)在位置24上,氨基酸残基为Z3;
(s)在位置104上,氨基酸残基为Z5;
(t)在位置52和/或69上,氨基酸残基为Z3;
(u)在位置14和/或119上,氨基酸残基为Z5;
(v)在位置10、32、63和/或83上,氨基酸残基为Z5;
(w)在位置48和/或80上,氨基酸残基为Z6;
(x)在位置40上,氨基酸残基为Z1或Z2;
(y)在位置96上,氨基酸残基为Z3或Z5;
(z)在位置65上,氨基酸残基为Z3、Z4或Z6;
(aa)在位置84和/或115上,氨基酸残基为Z3;
(ab)在位置93上,氨基酸残基为Z4;
(ac)在位置130上,氨基酸残基为Z2;
(ad)在位置58上,氨基酸残基为Z3、Z4或者Z6;
(ae)在位置47上,氨基酸残基为Z4或Z6;
(af)在位置49和/或100上,氨基酸残基为Z3或Z4;
(ag)在位置68上,氨基酸残基为Z4或Z5;
(ah)在位置143上,氨基酸残基为Z4;
(ai)在位置131上,氨基酸残基为Z5;
(aj)在位置125和/或128上,氨基酸残基为Z5;
(ak)在位置67上,氨基酸残基为Z3或Z4;
(al)在位置60上,氨基酸残基为Z5;和
(am)在位置37上,氨基酸残基为Z4或Z6;
其中Z1是选自由A、I、L、M和V组成的组的氨基酸残基;Z2是选自由F、W和Y组成的组的氨基酸残基;Z3是选自由N、Q、S和T组成的组的氨基酸;Z4是选自由R、H和K组成的组的氨基酸;Z5是选自由D和E组成的组的氨基酸残基;和Z6是选自由C、G和P组成的组的氨基酸残基。
4.权利要求1的分离的或重组的多核苷酸,其中氨基酸序列中对应于在(a)-(am)中指定的位置的氨基酸残基至少90%地遵从在(a)-(am)中指定的氨基酸残基的限制。
5.权利要求1-4的任何一项的分离的或重组的多核苷酸,其中氨基酸序列中对应于如下位置的氨基酸残基至少90%地遵从如下限制:
(a)在位置1、7、9、13、20、36、42、46、50、56、64、70、72、75、76、78、94、98、107、110、117、118、121和/或141上,氨基酸残基为B1;和
(b)在位置16、21、22、23、25、29、34、41、43、44、55、66、71、73、74、77、85、87、88、95、99、102、108、109、111、116、122、127、133、134、136和/或137上,氨基酸残基为B2;
其中B1为选自由A、I、L、M、F、W、Y和V组成的组的氨基酸;而B2是选自由R、N、D、C、Q、E、G、H、K、P、S和T组成的组的氨基酸。
6.权利要求1-4的任何一项的分离的或重组的多核苷酸,其中氨基酸序列中对应于如下位置的氨基酸残基至少90%地遵从如下限制:
(a)在位置1、7、9、13、20、42、46、50、56、64、70、72、75、76、78、94、98、107、110、117、118、121和/或141上,氨基酸残基为B1;和
(b)在位置16、21、22、23、25、29、34、36、41、43、44、55、66、71、73、74、77、85、87、88、95、99、102、108、109、111、116、122、127、133、134、136和/或137上,氨基酸残基为B2;
其中B1为选自由A、I、L、M、F、W、Y和V组成的组的氨基酸;而B2是选自由R、N、D、C、Q、E、G、H、K、P、S和T组成的组的氨基酸。
7.权利要求1-4的任何一项的分离的或重组的多核苷酸,其中氨基酸序列中对应于如下位置的氨基酸残基至少90%地遵从如下限制:
(a)在位置1、7、9、20、42、50、72、75、76、78、94、98、110、121和/或141上,氨基酸残基为Z1;
(b)在位置13、46、56、70、107、117和/或118上,氨基酸残基为Z2;
(c)在位置23、55、71、77、88和/或109上,氨基酸残基为Z3;
(d)在位置16、21、41、73、85、99和/或111上,氨基酸残基为Z4;
(e)在位置34和/或95上,氨基酸残基为Z5;
(f)在位置22,25,29,43,44、66、74、87、102、108、116、122、127、133、134、136和/或137上,氨基酸残基为Z6;
其中Z1是选自由A、I、L、M和V组成的组的氨基酸残基;Z2是选自由F、W和Y组成的组的氨基酸残基;Z3是选自由N、Q、S和T组成的组的氨基酸;Z4是选自由R、H和K组成的组的氨基酸;Z5是选自由D和E组成的组的氨基酸残基;和Z6是选自由C、G和P组成的组的氨基酸残基。
8.权利要求7的分离的或重组的多核苷酸,其中氨基酸序列在位置36上包含选自由Z1和Z3组成的组的氨基酸残基。
9.权利要求7或8的分离的或重组的多核苷酸,其中氨基酸序列在位置64上还包含选自由Z1和Z2组成的组的氨基酸残基。
10.权利要求1-9的任何一项的分离的或重组的多核苷酸,其中氨基酸序列中对应于如下位置的氨基酸残基至少80%地遵从如下限制:
(a)在位置2上,氨基酸残基为I或L;
(b)在位置3上,氨基酸残基为E;
(c)在位置4上,氨基酸残基为V或I;
(d)在位置5上,氨基酸残基为K;
(e)在位置6上,氨基酸残基为P;
(f)在位置8上,氨基酸残基为N;
(g)在位置10上,氨基酸残基为E;
(h)在位置11上,氨基酸残基为D或E;
(i)在位置12上,氨基酸残基为T;
(j)在位置14上,氨基酸残基为E或D;
(k)在位置15上,氨基酸残基为L;
(l)在位置17上,氨基酸残基为H;
(m)在位置18上,氨基酸残基为R、E或K;
(n)在位置19上,氨基酸残基为I或V;
(o)在位置24上,氨基酸残基为Q;
(p)在位置26上,氨基酸残基为M、L、V或I;
(q)在位置27上,氨基酸残基为E;
(r)在位置28上,氨基酸残基为A或V;
(s)在位置30上,氨基酸残基为M;
(t)在位置31上,氨基酸残基为Y或F;
(u)在位置32上,氨基酸残基为E或D;
(v)在位置33上,氨基酸残基为T或S;
(w)在位置35上,氨基酸残基为L;
(x)在位置37上,氨基酸残基为R、G、E或Q;
(y)在位置39上,氨基酸残基为A或S;
(z)在位置40上,氨基酸残基为F或L;
(aa)在位置45上,氨基酸残基为Y或F;
(ab)在位置47上,氨基酸残基为R或G;
(ac)在位置48上,氨基酸残基为G;
(ad)在位置49上,氨基酸残基为K、R或Q;
(ae)在位置51上,氨基酸残基为I或V;
(af)在位置52上,氨基酸残基为S;
(ag)在位置53上,氨基酸残基为I或V;
(ah)在位置54上,氨基酸残基为A;
(ai)在位置57上,氨基酸残基为H或N;
(aj)在位置58上,氨基酸残基为Q、K、R或P;
(ak)在位置59上,氨基酸残基为A;
(al)在位置60上,氨基酸残基为E;
(am)在位置61上,氨基酸残基为H或R;
(an)在位置63上,氨基酸残基为E或D;
(ao)在位置65上,氨基酸残基为E、P或Q;
(ap)在位置67上,氨基酸残基为Q或R;
(aq)在位置68上,氨基酸残基为K或E;
(ar)在位置69上,氨基酸残基为Q;
(as)在位置79上,氨基酸残基为E;
(at)在位置80上,氨基酸残基为G;
(au)在位置81上,氨基酸残基为Y、H或F;
(av)在位置82上,氨基酸残基为R;
(aw)在位置83上,氨基酸残基为E或D;
(ax)在位置84上,氨基酸残基为Q;
(ay)在位置86上,氨基酸残基为A;
(az)在位置89上,氨基酸残基为G、T或S;
(ba)在位置90上,氨基酸残基为L;
(bb)在位置91上,氨基酸残基为L、I或V;
(bc)在位置92上,氨基酸残基为R或K;
(bd)在位置93上,氨基酸残基为H;
(be)在位置96上,氨基酸残基为E或Q;
(bf)在位置97上,氨基酸残基为I;
(bg)在位置100上,氨基酸残基为K或N;
(bh)在位置101上,氨基酸残基为K或R;
(bi)在位置103上,氨基酸残基为A或V;
(bj)在位置104上,氨基酸残基为D;
(bk)在位置105上,氨基酸残基为M、L或I;
(bl)在位置106上,氨基酸残基为L;
(bm)在位置112上,氨基酸残基为T或A;
(bn)在位置113上,氨基酸残基为S或T;
(bo)在位置114上,氨基酸残基为A;
(bp)在位置115上,氨基酸残基为S;
(bq)在位置119上,氨基酸残基为K或R;
(br)在位置120上,氨基酸残基为K或R;
(bs)在位置123上,氨基酸残基为F或L;
(bt)在位置125上,氨基酸残基为E;
(bu)在位置126上,氨基酸残基为Q或H;
(bv)在位置128上,氨基酸残基为E或D;
(bw)在位置129上,氨基酸残基为V或I;
(bx)在位置130上,氨基酸残基为F;
(by)在位置131上,氨基酸残基为D或E;
(bz)在位置132上,氨基酸残基为T;
(ca)在位置135上,氨基酸残基为V;
(cb)在位置138上,氨基酸残基为H;
(cc)在位置139上,氨基酸残基为I;
(cd)在位置140上,氨基酸残基为L或M;
(ce)在位置142上,氨基酸残基为Y;
(cf)在位置143上,氨基酸残基为K或R;
(cg)在位置145上,氨基酸残基为L或I;和
(ch)在位置146上,氨基酸残基为T。
11.权利要求10的分离的或重组的多核苷酸,其中氨基酸序列中对应于在(a)-(ch)中指定的位置的氨基酸残基至少90%地遵从在(a)-(ch)中指定的氨基酸残基的限制。
12.权利要求1的分离的或重组的多核苷酸,其中氨基酸序列中对应于如下位置的氨基酸残基至少80%地遵从如下限制:
(a)在位置9、76、94和110上,氨基酸残基为A;
(b)在位置29和108上,氨基酸残基为C;
(c)在位置34上,氨基酸残基为D;
(d)在位置95上,氨基酸残基为E;
(e)在位置56上,氨基酸残基为F;
(f)在位置43、44、66、74、87、102、116、122、127和136上,氨基酸残基为G;
(g)在位置41上,氨基酸残基为H;
(h)在位置7上,氨基酸残基为I;
(i)在位置85上,氨基酸残基为K;
(j)在位置20、42、50、78和121上,氨基酸残基为L;
(k)在位置1和141上,氨基酸残基为M;
(l)在位置23和109上,氨基酸残基为N;
(m)在位置22、25、133、134和137上,氨基酸残基为P;
(n)在位置71上,氨基酸残基为Q;
(o)在位置16、21、73、99和111上,氨基酸残基为R;
(p)在位置55上,氨基酸残基为S;
(q)在位置77上,氨基酸残基为T;
(r)在位置107上,氨基酸残基为W;和
(s)在位置13、46、70和118上,氨基酸残基为Y。
13.权利要求1-12的任何一项的分离的或重组的多核苷酸,其中氨基酸序列包含选自由如下组成的组的氨基酸残基:
(a)在位置36上,氨基酸残基为M、L或T;
(b)在位置72上,氨基酸残基为L或I;
(c)在位置75上,氨基酸残基为M或V;
(d)在位置64上,氨基酸残基为L、I或F;
(e)在位置88上,氨基酸残基为T或S;和
(f)在位置117上,氨基酸残基为Y或F。
14.权利要求1-13的任何一项的分离的或重组的多核苷酸,其中氨基酸序列包含一个或多个选自由如下组成的组的氨基酸残基:
(a)在位置14上,氨基酸残基为D;
(b)在位置18上,氨基酸残基为E;
(c)在位置26上,氨基酸残基为M或V;
(e)在位置30上,氨基酸残基为I;
(f)在位置32上,氨基酸残基为D;
(g)在位置36上,氨基酸残基为M或T;
(i)在位置37上,氨基酸残基为C;
(j)在位置38上,氨基酸残基为D;
(j)在位置53上,氨基酸残基为V;
(k)在位置58上,氨基酸残基为R;
(l)在位置61上,氨基酸残基为R;
(m)在位置62上,氨基酸残基为L;
(n)在位置64上,氨基酸残基为I或F;
(o)在位置65上,氨基酸残基为P;
(p)在位置72上,氨基酸残基为I;
(q)在位置75上,氨基酸残基为V;
(r)在位置88上,氨基酸残基为T;
(s)在位置89上,氨基酸残基为G;
(t)在位置91上,氨基酸残基为L;
(u)在位置98上,氨基酸残基为I;
(v)在位置105上,氨基酸残基为I;
(w)在位置112上,氨基酸残基为A;
(x)在位置124上,氨基酸残基为G或C;
(y)在位置128上,氨基酸残基为D;
(z)在位置140上,氨基酸残基为M;
(aa)在位置143上,氨基酸残基为R;和
(ab)在位置144上,氨基酸残基为W。
15.权利要求14的分离的或重组的多核苷酸,其中氨基酸序列中对应于在(a)-(ab)中指定的位置的氨基酸残基至少80%地遵从在(a)-(ab)中指定的氨基酸残基的限制。
16.权利要求1-15的任何一项的分离的或重组的多核苷酸,其中氨基酸序列包含一个或多个选自由如下组成的组的氨基酸残基:
(a)在位置41上,氨基酸残基为H;
(b)在位置138上,氨基酸残基为H;
(c)在位置34上,氨基酸残基为N;和
(d)在位置55上,氨基酸残基为S。
17.一种分离的或重组的多核苷酸,包含选自由如下组成的组的核苷酸序列:
(a)编码与SEQ ID NO:577至少98%相同的氨基酸序列的核苷酸序列;
(b)编码与SEQ ID NO:578至少97%相同的氨基酸序列的核苷酸序列;
(c)编码与SEQ ID NO:621至少97%相同的氨基酸序列的核苷酸序列;
(d)编码与SEQ ID NO:579至少98%相同的氨基酸序列的核苷酸序列;
(e)编码与SEQ ID NO:602至少98,%相同的氨基酸序列的核苷酸序列;
(f)编码与SEQ ID NO:697至少95%相同的氨基酸序列的核苷酸序列;
(g)编码与SEQ ID NO:712至少96%相同的氨基酸序列的核苷酸序列;
(h)编码与SEQ ID NO:613至少97%相同的氨基酸序列的核苷酸序列;
(i)编码与SEQ ID NO:677至少89%相同的氨基酸序列的核苷酸序列;
(j)编码与SEQ ID NO:584至少95%相同的氨基酸序列的核苷酸序列;
(k)编码与SEQ ID NO:707至少98%相同的氨基酸序列的核苷酸序列;
(l)编码与SEQ ID NO:616至少98%相同的氨基酸序列的核苷酸序列;
(m)编码与SEQ ID NO:612至少96%相同的氨基酸序列的核苷酸序列;
(n)编码与SEQ ID NO:590至少98%相同的氨基酸序列的核苷酸序列;
(o)与(a)-(n)的任何之一互补的核苷酸序列。
19.权利要求17的分离的或重组的多核苷酸,其中氨基酸序列中对应于如下位置的氨基酸残基至少90%地遵从如下限制:
(a)在位置2、4、15、19、26、28、31、45、51、54、86、90、91、97、103、105、106、114、123、129、139和/或145上,氨基酸残基为B1;和
(b)在位置3、5、8、10、11、14、17、24、27、32、37、47、48、49、52、57、58、61、63、68、69、79、80、82、83、89、92、100、101、104、119、120、125、126、128、131和/或143上,氨基酸残基为B2;
其中B1为选自由A、I、L、M、F、W、Y和V组成的组的氨基酸;而B2是选自由R、N、D、C、Q、E、G、H、K、P、S和T组成的组的氨基酸。
20.权利要求17的分离的或重组的多核苷酸,其中氨基酸序列中对应于如下位置的氨基酸残基至少80%地遵从如下限制:
(a)在位置2、4、15、19、26、28、51、54、86、90、91、97、103、105、106、114、129、139和/或145上,氨基酸残基为Z1;
(b)在位置31和/或45上,氨基酸残基为Z2;
(c)在位置8上,氨基酸残基为Z3;
(d)在位置89上,氨基酸残基为Z3或者Z6;
(e)在位置82、92、101和/或120上,氨基酸残基为Z4;
(f)在位置3、11、27和/或79上,氨基酸残基为Z5;
(g)在位置18上,氨基酸残基为Z4或Z5;
(h)在位置123上,氨基酸残基为Z1或Z2;
(i)在位置12、33、35、39、53、59、112、132、135、140和/或146上,氨基酸残基为Z1或Z3;
(j)在位置30上,氨基酸残基为Z1;
(k)在位置6上,氨基酸残基为Z6;
(l)在位置81上,氨基酸残基为Z2或Z4;
(m)在位置113上,氨基酸残基为Z3;
(n)在位置138上,氨基酸残基为Z4;
(o)在位置142上,氨基酸残基为Z2;
(p)在位置57和/或126上,氨基酸残基为Z3或Z4;
(q)在位置5、17和61上,氨基酸残基为Z4;
(r)在位置24上,氨基酸残基为Z3;
(s)在位置104上,氨基酸残基为Z5;
(t)在位置52和/或69上,氨基酸残基为Z3;
(u)在位置14和/或119上,氨基酸残基为Z5;
(v)在位置10、32、63和/或83上,氨基酸残基为Z5;
(w)在位置48和/或80上,氨基酸残基为Z6;
(x)在位置40上,氨基酸残基为Z1或Z2;
(y)在位置96上,氨基酸残基为Z3或Z5;
(z)在位置65上,氨基酸残基为Z3、Z4或Z6;
(aa)在位置84和/或115上,氨基酸残基为Z3;
(ab)在位置93上,氨基酸残基为Z4;
(ac)在位置130上,氨基酸残基为Z2;
(ad)在位置58上,氨基酸残基为Z3、Z4或者Z6;
(ae)在位置47上,氨基酸残基为Z4或Z6;
(af)在位置49和/或100上,氨基酸残基为Z3或Z4;
(ag)在位置68上,氨基酸残基为Z4或Z5;
(ah)在位置143上,氨基酸残基为Z4;
(ai)在位置131上,氨基酸残基为Z5;
(aj)在位置125和/或128上,氨基酸残基为Z5;
(ak)在位置67上,氨基酸残基为Z3或Z4;
(al)在位置60上,氨基酸残基为Z5;和
(am)在位置37上,氨基酸残基为Z4或Z6;
其中Z1是选自由A、I、L、M和V组成的组的氨基酸残基;Z2是选自由F、W和Y组成的组的氨基酸残基;Z3是选自由N、Q、S和T组成的组的氨基酸;Z4是选自由R、H和K组成的组的氨基酸;Z5是选自由D和E组成的组的氨基酸残基;和Z6是选自由C、G和P组成的组的氨基酸残基。
21.权利要求20的分离的或重组的多核苷酸,其中氨基酸序列中对应于在(a)-(am)中指定的位置的氨基酸残基至少90%地遵从于在(a)-(am)中指定的氨基酸残基的限制。
22.权利要求17-21的任何一项的分离的或重组的多核苷酸,其中氨基酸序列中对应于如下位置的氨基酸残基至少90%地遵从于如下限制:
(a)在位置1、7、9、13、20、36、42、46、50、56、64、70、72、75、76、78、94、98、107、110、117、118、121和/或141上,氨基酸残基为B1;和
(b)在位置16、21、22、23、25、29、34、41、43、44、55、66、71、73、74、77、85、87、88、95、99、102、108、109、111、116、122、127、133、134、136和/或137上,氨基酸残基为B2;
其中B1为选自由A、I、L、M、F、W、Y和V组成的组的氨基酸;而B2是选自由R、N、D、C、Q、E、G、H、K、P、S和T组成的组的氨基酸。
23.权利要求17-22的任何一项的分离的或重组的多核苷酸,其中氨基酸序列中对应于如下位置的氨基酸残基至少90%地遵从于如下限制:
(a)在位置1、7、9、13、20、42、46、50、56、64、70、72、75、76、78、94、98、107、110、117、118、121和/或141上,氨基酸残基为B1;和
(b)在位置16、21、22、23、25、29、34、36、41、43、44、55、66、71、73、74、77、85、87、88、95、99、102、108、109、111、116、122、127、133、134、136和/或137上,氨基酸残基为B2;
其中B1为选自由A、I、L、M、F、W、Y和V组成的组的氨基酸;而B2是选自由R、N、D、C、Q、E、G、H、K、P、S和T组成的组的氨基酸。
24.权利要求17-22的任何一项的分离的或重组的多核苷酸,其中氨基酸序列中对应于如下位置的氨基酸残基至少90%地遵从于如下限制:
(a)在位置1、7、9、20、42、50、72、75、76、78、94、98、110、121和/或141上,氨基酸残基为Z1;
(b)在位置13、46、56、70、107、117和/或118上,氨基酸残基为Z2;
(c)在位置23、55、71、77、88和/或109上,氨基酸残基为Z3;
(d)在位置16、21、41、73、85、99和/或111上,氨基酸残基为Z4;
(e)在位置34和/或95上,氨基酸残基为Z5;
(f)在位置22,25,29,43,44、66、74、87、102、108、116、122、127、133、134、136和/或137上,氨基酸残基为Z6;
其中Z1是选自由A、I、L、M和V组成的组的氨基酸残基;Z2是选自由F、W和Y组成的组的氨基酸残基;Z 3是选自由N、Q、S和T组成的组的氨基酸;Z4是选自由R、H和K组成的组的氨基酸;Z5是选自由D和E组成的组的氨基酸残基;和Z6是选自由C、G和P组成的组的氨基酸残基。
25.权利要求24的分离的或重组的多核苷酸,其中氨基酸序列在位置36上包含选自由Z1和Z3组成的组的氨基酸残基。
26.权利要求24或25的分离的或重组的多核苷酸,其中氨基酸序列在位置64上还包含选自由Z1和Z2组成的组的氨基酸残基。
27.权利要求17-26的任何一项的分离的或重组的多核苷酸,其中氨基酸序列中对应于如下位置的氨基酸残基至少80%地遵从如下限制:
(a)在位置2上,氨基酸残基为I或L;
(b)在位置3上,氨基酸残基为E;
(c)在位置4上,氨基酸残基为V或I;
(d)在位置5上,氨基酸残基为K;
(e)在位置6上,氨基酸残基为P;
(f)在位置8上,氨基酸残基为N;
(g)在位置10上,氨基酸残基为E;
(h)在位置11上,氨基酸残基为D或E;
(i)在位置12上,氨基酸残基为T;
(j)在位置14上,氨基酸残基为E或D;
(k)在位置15上,氨基酸残基为L;
(l)在位置17上,氨基酸残基为H;
(m)在位置18上,氨基酸残基为R、E或K;
(n)在位置19上,氨基酸残基为I或V;
(o)在位置24上,氨基酸残基为Q;
(p)在位置26上,氨基酸残基为M、L、V或I;
(q)在位置27上,氨基酸残基为E;
(r)在位置28上,氨基酸残基为A或V;
(s)在位置30上,氨基酸残基为M;
(t)在位置31上,氨基酸残基为Y或F;
(u)在位置32上,氨基酸残基为E或D;
(v)在位置33上,氨基酸残基为T或S;
(w)在位置35上,氨基酸残基为L;
(x)在位置37上,氨基酸残基为R、G、E或Q;
(y)在位置39上,氨基酸残基为A或S;
(z)在位置40上,氨基酸残基为F或L;
(aa)在位置45上,氨基酸残基为Y或F;
(ab)在位置47上,氨基酸残基为R或G;
(ac)在位置48上,氨基酸残基为G;
(ad)在位置49上,氨基酸残基为K、R或Q;
(ae)在位置51上,氨基酸残基为I或V;
(af)在位置52上,氨基酸残基为S;
(ag)在位置53上,氨基酸残基为I或V;
(ah)在位置54上,氨基酸残基为A;
(ai)在位置57上,氨基酸残基为H或N;
(aj)在位置58上,氨基酸残基为Q、K、R或P;
(ak)在位置59上,氨基酸残基为A;
(al)在位置60上,氨基酸残基为E;
(am)在位置61上,氨基酸残基为H或R;
(an)在位置63上,氨基酸残基为E或D;
(ao)在位置65上,氨基酸残基为E、P或Q;
(ap)在位置67上,氨基酸残基为Q或R;
(aq)在位置68上,氨基酸残基为K或E;
(ar)在位置69上,氨基酸残基为Q;
(as)在位置79上,氨基酸残基为E;
(at)在位置80上,氨基酸残基为G;
(au)在位置81上,氨基酸残基为Y、H或F;
(av)在位置82上,氨基酸残基为R;
(aw)在位置83上,氨基酸残基为E或D;
(ax)在位置84上,氨基酸残基为Q;
(ay)在位置86上,氨基酸残基为A;
(az)在位置89上,氨基酸残基为G、T或S;
(ba)在位置90上,氨基酸残基为L;
(bb)在位置91上,氨基酸残基为L、I或V;
(bc)在位置92上,氨基酸残基为R或K;
(bd)在位置93上,氨基酸残基为H;
(be)在位置96上,氨基酸残基为E或Q;
(bf)在位置97上,氨基酸残基为I;
(bg)在位置100上,氨基酸残基为K或N;
(bh)在位置101上,氨基酸残基为K或R;
(bi)在位置103上,氨基酸残基为A或V;
(bj)在位置104上,氨基酸残基为D;
(bk)在位置105上,氨基酸残基为M、L或I;
(bl)在位置106上,氨基酸残基为L;
(bm)在位置112上,氨基酸残基为T或A;
(bn)在位置113上,氨基酸残基为S或T;
(bo)在位置114上,氨基酸残基为A;
(bp)在位置115上,氨基酸残基为S;
(bq)在位置119上,氨基酸残基为K或R;
(br)在位置120上,氨基酸残基为K或R;
(bs)在位置123上,氨基酸残基为F或L;
(bt)在位置125上,氨基酸残基为E;
(bu)在位置126上,氨基酸残基为Q或H;
(bv)在位置128上,氨基酸残基为E或D;
(bw)在位置129上,氨基酸残基为V或I;
(bx)在位置130上,氨基酸残基为F;
(by)在位置131上,氨基酸残基为D或E;
(bx)在位置132上,氨基酸残基为T;
(ca)在位置135上,氨基酸残基为V;
(cb)在位置138上,氨基酸残基为H;
(cc)在位置139上,氨基酸残基为I;
(cd)在位置140上,氨基酸残基为L或M;
(ce)在位置142上,氨基酸残基为Y;
(cf)在位置143上,氨基酸残基为K或R;
(cg)在位置145上,氨基酸残基为L或I;和
(ch)在位置146上,氨基酸残基为T。
28.权利要求27的分离的或重组的多核苷酸,其中氨基酸序列中对应于在(a)-(ch)中指定的位置的氨基酸残基至少90%地遵从在(a)-(ch)中指定的氨基酸残基的限制。
29.权利要求17的分离的或重组的多核苷酸,其中氨基酸序列中对应于如下位置的氨基酸残基至少80%地遵从如下限制:
(a)在位置9、76、94和110上,氨基酸残基为A;
(b)在位置29和108上,氨基酸残基为C;
(c)在位置34上,氨基酸残基为D;
(d)在位置95上,氨基酸残基为E;
(e)在位置56上,氨基酸残基为F;
(f)在位置43、44、66、74、87、102、116、122、127和136上,氨基酸残基为G;
(g)在位置41上,氨基酸残基为H;
(h)在位置7上,氨基酸残基为I;
(i)在位置85上,氨基酸残基为K;
(j)在位置20、42、50、78和121上,氨基酸残基为L;
(k)在位置1和141上,氨基酸残基为M;
(l)在位置23和109上,氨基酸残基为N;
(m)在位置22、25、133、134和137上,氨基酸残基为P;
(n)在位置71上,氨基酸残基为Q;
(o)在位置16、21、73、99和111上,氨基酸残基为R;
(p)在位置55上,氨基酸残基为S;
(q)在位置77上,氨基酸残基为T;
(r)在位置107上,氨基酸残基为W;和
(s)在位置13、46、70和118上,氨基酸残基为Y。
29.权利要求17的分离的或重组的多核苷酸,其中氨基酸序列包含一个或多个选自由如下组成的组的氨基酸残基:
(a)在位置14上,氨基酸残基为D;
(b)在位置18上,氨基酸残基为E;
(c)在位置26上,氨基酸残基为M或V;
(e)在位置30上,氨基酸残基为I;
(f)在位置32上,氨基酸残基为D;
(g)在位置36上,氨基酸残基为M或T;
(i)在位置37上,氨基酸残基为C;
(j)在位置38上,氨基酸残基为D;
(j)在位置53上,氨基酸残基为V;
(k)在位置58上,氨基酸残基为R;
(l)在位置61上,氨基酸残基为R;
(m)在位置62上,氨基酸残基为L;
(n)在位置64上,氨基酸残基为I或F;
(o)在位置65上,氨基酸残基为P;
(p)在位置72上,氨基酸残基为I;
(q)在位置75上,氨基酸残基为V;
(r)在位置88上,氨基酸残基为T;
(s)在位置89上,氨基酸残基为G;
(t)在位置91上,氨基酸残基为L;
(u)在位置98上,氨基酸残基为I;
(v)在位置105上,氨基酸残基为I;
(w)在位置112上,氨基酸残基为A;
(x)在位置124上,氨基酸残基为G或C;
(y)在位置128上,氨基酸残基为D;
(z)在位置140上,氨基酸残基为M;
(aa)在位置143上,氨基酸残基为R;和
(ab)在位置144上,氨基酸残基为W。
31.权利要求30的分离的或重组的多核苷酸,其中氨基酸序列中对应于在(a)-(ab)中指定的位置的氨基酸残基至少80%地遵从在(a)-(ab)中指定的氨基酸残基的限制。
32.权利要求17-31的任何一项的分离的或重组的多核苷酸,其中氨基酸序列包含一个或多个选自由如下组成的组的氨基酸残基:
(a)在位置41上,氨基酸残基为H;
(b)在位置138上,氨基酸残基为H;
(c)在位置34上,氨基酸残基为N;和
(e)在位置55上,氨基酸残基为S。
33.一种分离的或重组的多核苷酸,其编码具有草甘膦-N-乙酰转移酶活性的蛋白质,所述多核苷酸选自由如下组成的组:SEQ ID NO:516、517、518、519、520、521、522、523、524、525、526、527、528、529、530、531、532、533、534、535、536、537、538、539、540、541、542、543、544、545、546、547、548、549、550、551、552、553、554、555、556、557、558、559、560、561、562、563、564、565、566、567、620、622、624、626、628、630、632、634、636、638、640、642、644、646、648、650、652、654、656、658、660、662、664、666、668、670、672、674、676、678、680、682、684、686、688、690、692、694、696、698、700、702、704、706、708、710、712、714、716、718、720、722、724、726、728、730、732、734、736、738、740、742、744、746、748、750、752、754、756、758、760、762、764、768、770、772、774、776、778、780、782、784、786、788、790、792、794、796、798、800、802、804、806、808、810、812、814、816、818、820、822、824、832、834、836、838、840、842、844、846、848、850、852、854、856、858、860、862、864、866、868、870、872、874、876、878、880、882、884、886、888、890、892、894、896、898、900、902、904、906、908、910、912、914、916、918、920、922、924、926、928、930、932、933、934、935、936、937、938、939、940、941、942、943、944、945、947、949、951和952。
34.权利要求1的分离的或重组的多核苷酸,其中由所述多核苷酸编码的多肽具有草甘膦-N-乙酰转移酶活性。
35.权利要求1的分离的或重组的多核苷酸,其中由所述多核苷酸编码的多肽具有草甘膦-N-酰基转移酶活性。
36.权利要求34的分离的或重组的多核苷酸,其中该多肽以对于草甘膦至少为10mM-1的k催化/Km催化草甘膦的乙酰化。
37.权利要求34的分离的或重组的多核苷酸,其中该多肽催化氨基甲基膦酸的乙酰化。
38.包含权利要求1的分离的或重组的多核苷酸的核酸构建体。
39.权利要求38的核酸构建体,它包含可操作地连接到权利要求1的分离的或重组的多核苷酸上的启动子,其中该启动子相对于所述多核苷酸是异源的,并且有效地引起被编码的多肽的充分表达,以提高用所述核酸构建体转化的植物细胞的草甘膦耐受性。
40.权利要求38的核酸构建体,其中权利要求1的分离的或重组的多核苷酸序列作为一种可选择的标记而发挥作用。
41.权利要求38的核酸构建体,其中该构建体是一种载体。
42.权利要求41的构建体,还包括编码第二种多肽的第二条多核苷酸序列,该第二种多肽赋予有效地表达第二种多肽的细胞或生物体以一种可检测的表型特征。
43.权利要求42的载体,其中可检测的表型特征作为可检测的标记而发挥作用。
44.权利要求42的载体,其中可检测的表型特征选自由除草剂抗性和可视标记组成的组。
45.权利要求41的载体,其中该载体还包含T-DNA序列。
46.权利要求41的载体,其中分离的或重组的多核苷酸被可操作地连接到调控序列上。
47.权利要求41的载体,其中该载体是植物转化载体。
48.包含至少一种权利要求1的分离的或重组的多核苷酸的细胞,其中该多核苷酸与细胞是异源的。
49.权利要求48的细胞,其中分离的或重组的多核苷酸被可操作地连接到调控序列上。
50.被权利要求41的载体转导的细胞。
51.权利要求49或50的细胞,其中该细胞是一种转基因植物细胞。
52.权利要求51的转基因植物细胞,其中转基因植物细胞表达一种具有草甘膦-N-乙酰转移酶活性的外源性多肽。
53.权利要求51的转基因植物细胞,其中转基因植物细胞表达一种具有草甘膦-N-酰基转移酶活性的外源性多肽。
54.一种包含权利要求52的细胞的转基因植物或转基因植物外植体。
55.一种包含权利要求53的细胞的转基因植物或转基因植物外植体。
56.权利要求54的转基因植物或转基因植物外植体,其中所述植物或植物外植体表达一种具有草甘膦-N-乙酰转移酶活性的多肽。
57.权利要求55的转基因植物或转基因植物外植体,其中所述植物或植物外植体表达一种具有草甘膦-N-酰基转移酶活性的多肽。
58.权利要求56的转基因植物或转基因植物外植体,其中所述的植物或植物外植体是一种农作物,其选自:Eleusine、黑麦草属(Lollium)、山白竹属(Bambusa)、芸苔属(Brassica)、鸭茅属(Dactylis)、高粱属(Sorghum)、狼尾草属(Pennisetum)、玉蜀黍属(Zea)、稻属(Oryza)、小麦属(Triticum)、黑麦属(Secale)、燕麦属(Avefza)、大麦属(Hordeuna)、甘蔗属(Saccharum)、薏苡属(Coix)、大豆属(Glycine)和棉属(Gossypium)。
59.权利要求57的转基因植物或转基因植物外植体,其中所述的植物或植物外植体是一种农作物,其选自:Eleusine、黑麦草属(Lollium)、山白竹属(Bambusa)、芸苔属(Brassica)、鸭茅属(Dactylis)、高粱属(Sorghum)、狼尾草属(Pennisetum)、玉蜀黍属(Zea)、稻属(Oryza)、小麦属(Triticum)、黑麦属(Secale)、燕麦属(Avefza)、大麦属(Hordeuna)、甘蔗属(Saccharum)、薏苡属(Coix)、大豆属(Glycine)和棉属(Gossypium)。
60.权利要求56的转基因植物或转基因植物外植体,其中所述的植物或植物外植体是鼠耳芥属(Arabidopsis)。
61.权利要求57的转基因植物或转基因植物外植体,其中所述的植物或植物外植体是鼠耳芥属(Arabidopsis)。
62.权利要求56的转基因植物或转基因植物外植体,其中所述的植物或植物外植体是棉属(Gossypium)。
63.权利要求57的转基因植物或转基因植物外植体,其中所述的植物或植物外植体是棉属(Gossypium)。
64.权利要求56的转基因植物或转基因植物外植体,其中与同一物种、植株或者品种相比,所述的植物或植物外植体具有增强的草甘膦抗性。
65.权利要求57的转基因植物或转基因植物外植体,其中与同一物种、植株或者品种相比,所述的植物或植物外植体具有增强的草甘膦抗性。
66.由权利要求56的植物生产的种子。
67.由权利要求57的植物生产的种子。
68.含有异源基因的转基因植物,所述基因选自由如下组成的组:SEQ ID NO:516、517、518、519、520、521、522、523、524、525、526、527、528、529、530、531、532、533、534、535、536、537、538、539、540、541、542、543、544、545、546、547、548、549、550、551、552、553、554、555、556、557、558、559、560、561、562、563、564、565、566、567、620、622、624、626、628、630、632、634、636、638、640、642、644、646、648、650、652、654、656、658、660、662、664、666、668、670、672、674、676、678、680、682、684、686、688、690、692、694、696、698、700、702、704、706、708、710、712、714、716、718、720、722、724、726、728、730、732、734、736、738、740、742、744、746、748、750、752、754、756、758、760、762、764、768、770、772、774、776、778、780、782、784、786、788、790、792、794、796、798、800、802、804、806、808、810、812、814、816、818、820、822、824、832、834、836、838、840、842、844、846、848、850、852、854、856、858、860、862、864、866、868、870、872、874、876、878、880、882、884、886、888、890、892、894、896、898、900、902、904、906、908、910、912、914、916、918、920、922、924、926、928、930、932、933、934、935、936、937、938、939、940、941、942、943、944、945、947、949、951和952,其编码对草甘膦具有至少为10mM-1min-1的k催化/Km的草甘膦-N-乙酰转移酶,其中该植物对以足以抑制缺乏异源基因的相同植物的生长的含量应用的草甘膦具有耐受性,而不会由于除草剂的应用而显著地降低产量。
69.权利要求68的转基因植物,其中草甘膦-N-乙酰转移酶催化氨基甲基膦酸的乙酰化。
70.一种分离的或重组的多肽,包含:
(a)一种氨基酸序列,采用BLOSUM62矩阵,存在一个缺口罚11分,延伸一个缺口罚1分,将该氨基酸与选自由SEQ ID NO:300、SEQ IDNO:445和SEQ ID NO:457组成的组的序列最佳地比对时,产生至少为460的相似性分值,其中该氨基酸序列包含一个或多个选自由如下组成的组的氨基酸残基:
(i)在位置18和38上的Z5氨基酸残基;
(ii)在位置62上的Z1氨基酸残基;
(iii)在位置124上的Z6氨基酸残基;和
(iv)在位置144上的Z2氨基酸残基,其中:
Z1为选自由A、I、L、M和V组成的组的氨基酸残基;
Z2为选自由F、W、和Y组成的组的氨基酸残基;
Z5为选自由D和E组成的组的氨基酸残基;
Z6为选自由C、G和P组成的组的氨基酸残基;或者
(b)其互补的核苷酸序列。
71.权利要求70的分离的或重组的多肽,其中氨基酸序列中对应于如下位置的氨基酸残基至少90%地遵从如下限制:
(a)在位置2、4、15、19、26、28、31、45、51、54、86、90、91、97、103、105、106、114、123、129、139和/或145上,氨基酸残基为B1;和
(b)在位置3、5、8、10、11、14、17、24、27、32、37、47、48、49、52、57、58、61、63、68、69、79、80、82、83、89、92、100、101、104、119、120、125、126、128、131和/或143上,氨基酸残基为B2;
其中B1为选自由A、I、L、M、F、W、Y和V组成的组的氨基酸;而B2是选自由R、N、D、C、Q、E、G、H、K、P、S和T组成的组的氨基酸。
72.权利要求70的分离的或重组的多肽,其中氨基酸序列中对应于如下位置的氨基酸残基至少80%地遵从如下限制:
(a)在位置2、4、15、19、26、28、51、54、86、90、91、97、103、105、106、114、129、139和/或145上,氨基酸残基为Z1;
(b)在位置31和/或45上,氨基酸残基为Z2;
(c)在位置8上,氨基酸残基为Z3;
(d)在位置89上,氨基酸残基为Z3或者Z6;
(e)在位置82、92、101和/或120上,氨基酸残基为Z4;
(f)在位置3、11、27和/或79上,氨基酸残基为Z5;
(g)在位置18上,氨基酸残基为Z4或Z5;
(h)在位置123上,氨基酸残基为Z1或Z2;
(i)在位置12、33、35、39、53、59、112、132、135、140和/或146上,氨基酸残基为Z1或Z3;
(j)在位置30上,氨基酸残基为Z1;
(k)在位置6上,氨基酸残基为Z6;
(l)在位置81上,氨基酸残基为Z2或Z4;
(m)在位置113上,氨基酸残基为Z3;
(n)在位置138上,氨基酸残基为Z4;
(o)在位置142上,氨基酸残基为Z2;
(p)在位置57和/或126上,氨基酸残基为Z3或Z4;
(q)在位置5、17和61上,氨基酸残基为Z4;
(r)在位置24上,氨基酸残基为Z3;
(s)在位置104上,氨基酸残基为Z5;
(t)在位置52和/或69上,氨基酸残基为Z3;
(u)在位置14和/或119上,氨基酸残基为Z5;
(v)在位置10、32、63和/或83上,氨基酸残基为Z5;
(w)在位置48和/或80上,氨基酸残基为Z6;
(x)在位置40上,氨基酸残基为Z1或Z2;
(y)在位置96上,氨基酸残基为Z3或Z5;
(z)在位置65上,氨基酸残基为Z3、Z4或Z6;
(aa)在位置84和/或115上,氨基酸残基为Z3;
(ab)在位置93上,氨基酸残基为Z4;
(ac)在位置130上,氨基酸残基为Z2;
(ad)在位置58上,氨基酸残基为Z3、Z4或者Z6;
(ae)在位置47上,氨基酸残基为Z4或Z6;
(af)在位置49和/或100上,氨基酸残基为Z3或Z4;
(ag)在位置68上,氨基酸残基为Z4或Z5;
(ah)在位置143上,氨基酸残基为Z4;
(ai)在位置131上,氨基酸残基为Z5;
(aj)在位置125和/或128上,氨基酸残基为Z5;
(ak)在位置67上,氨基酸残基为Z3或Z4;
(al)在位置60上,氨基酸残基为Z5;和
(am)在位置37上,氨基酸残基为Z4或Z6;
其中Z1是选自由A、I、L、M和V组成的组的氨基酸残基;Z2是选自由F、W和Y组成的组的氨基酸残基;Z3是选自由N、Q、S和T组成的组的氨基酸;Z4是选自由R、H和K组成的组的氨基酸;Z5是选自由D和E组成的组的氨基酸残基;和Z6是选自由C、G和P组成的组的氨基酸残基。
73.权利要求72的分离的或重组的多肽,其中氨基酸序列中对应于在(a)-(am)中指定的位置的氨基酸残基至少90%地遵从在(a)-(am)中指定的氨基酸残基的限制。
74.权利要求70-73的任何一项的分离的或重组的多肽,其中氨基酸序列中对应于如下位置的氨基酸残基至少90%地遵从如下限制:
(a)在位置1、7、9、13、20、36、42、46、50、56、64、70、72、75、76、78、94、98、107、110、117、118、121和/或141上,氨基酸残基为B1;和
(b)在位置16、21、22、23、25、29、34、41、43、44、55、66、71、73、74、77、85、87、88、95、99、102、108、109、111、116、122、127、133、134、136和/或137上,氨基酸残基为B2;
其中B1为选自由A、I、L、M、F、W、Y和V组成的组的氨基酸;而B2是选自由R、N、D、C、Q、E、G、H、K、P、S和T组成的组的氨基酸。
75.权利要求70-73的任何一项的分离的或重组的多肽,其中氨基酸序列中对应于如下位置的氨基酸残基至少90%地遵从如下限制:
(a)在位置1、7、9、13、20、42、46、50、56、64、70、72、75、76、78、94、98、107、110、117、118、121和/或141上,氨基酸残基为B1;和
(b)在位置16、21、22、23、25、29、34、36、41、43、44、55、66、71、73、74、77、85、87、88、95、99、102、108、109、111、116、122、127、133、134、136和/或137上,氨基酸残基为B2;
其中B1为选自由A、I、L、M、F、W、Y和V组成的组的氨基酸;而B2是选自由R、N、D、C、Q、E、G、H、K、P、S和T组成的组的氨基酸。
76.权利要求70-73的任何一项的分离的或重组的多肽,其中氨基酸序列中对应于如下位置的氨基酸残基至少90%地遵从如下限制:
(a)在位置1、7、9、20、42、50、72、75、76、78、94、98、110、121和/或141上,氨基酸残基为Z1;
(b)在位置13、46、56、70、107、117和/或118上,氨基酸残基为Z2;
(c)在位置23、55、71、77、88和/或109上,氨基酸残基为Z3;
(d)在位置16、21、41、73、85、99和/或111上,氨基酸残基为Z4;
(e)在位置34和/或95上,氨基酸残基为Z5;
(f)在位置22,25,29,43,44、66、74、87、102、108、116、122、127、133、134、136和/或137上,氨基酸残基为Z6;
其中Z1是选自由A、I、L、M和V组成的组的氨基酸残基;Z2是选自由F、W和Y组成的组的氨基酸残基;Z3是选自由N、Q、S和T组成的组的氨基酸;Z4是选自由R、H和K组成的组的氨基酸;Z5是选自由D和E组成的组的氨基酸残基;和Z6是选自由C、G和P组成的组的氨基酸残基。
77.权利要求76的分离的或重组的多肽,其中氨基酸序列在位置36上包含选自由Z1和Z3组成的组的氨基酸残基。
78.权利要求76的分离的或重组的多肽,其中氨基酸序列在位置64上还包含选自由Z1和Z2组成的组的氨基酸残基。
79.权利要求70-78的任何一项的分离的或重组的多肽,其中氨基酸序列中对应于如下位置的氨基酸残基至少80%地遵从如下限制:
(a)在位置2上,氨基酸残基为I或L;
(b)在位置3上,氨基酸残基为E;
(c)在位置4上,氨基酸残基为V或I;
(d)在位置5上,氨基酸残基为K;
(e)在位置6上,氨基酸残基为P;
(f)在位置8上,氨基酸残基为N;
(g)在位置10上,氨基酸残基为E;
(h)在位置11上,氨基酸残基为D或E;
(i)在位置12上,氨基酸残基为T;
(j)在位置14上,氨基酸残基为E或D;
(k)在位置15上,氨基酸残基为L;
(l)在位置17上,氨基酸残基为H;
(m)在位置18上,氨基酸残基为R、E或K;
(n)在位置19上,氨基酸残基为I或V;
(o)在位置24上,氨基酸残基为Q;
(p)在位置26上,氨基酸残基为M、L、V或I;
(q)在位置27上,氨基酸残基为E;
(r)在位置28上,氨基酸残基为A或V;
(s)在位置30上,氨基酸残基为M;
(t)在位置31上,氨基酸残基为Y或F;
(u)在位置32上,氨基酸残基为E或D;
(v)在位置33上,氨基酸残基为T或S;
(w)在位置35上,氨基酸残基为L;
(x)在位置37上,氨基酸残基为R、G、E或Q;
(y)在位置39上,氨基酸残基为A或S;
(z)在位置40上,氨基酸残基为F或L;
(aa)在位置45上,氨基酸残基为Y或F;
(ab)在位置47上,氨基酸残基为R或G;
(ac)在位置48上,氨基酸残基为G;
(ad)在位置49上,氨基酸残基为K、R或Q;
(ae)在位置51上,氨基酸残基为I或V;
(af)在位置52上,氨基酸残基为S;
(ag)在位置53上,氨基酸残基为I或V;
(ah)在位置54上,氨基酸残基为A;
(ai)在位置57上,氨基酸残基为H或N;
(aj)在位置58上,氨基酸残基为Q、K、R或P;
(ak)在位置59上,氨基酸残基为A;
(al)在位置60上,氨基酸残基为E;
(am)在位置61上,氨基酸残基为H或R;
(an)在位置63上,氨基酸残基为E或D;
(ao)在位置65上,氨基酸残基为E、P或Q;
(ap)在位置67上,氨基酸残基为Q或R;
(aq)在位置68上,氨基酸残基为K或E;
(ar)在位置69上,氨基酸残基为Q;
(as)在位置79上,氨基酸残基为E;
(at)在位置80上,氨基酸残基为G;
(au)在位置81上,氨基酸残基为Y、H或F;
(av)在位置82上,氨基酸残基为R;
(aw)在位置83上,氨基酸残基为E或D;
(ax)在位置84上,氨基酸残基为Q;
(ay)在位置86上,氨基酸残基为A;
(az)在位置89上,氨基酸残基为G、T或S;
(ba)在位置90上,氨基酸残基为L;
(bb)在位置91上,氨基酸残基为L、I或V;
(bc)在位置92上,氨基酸残基为R或K;
(bd)在位置93上,氨基酸残基为H;
(be)在位置96上,氨基酸残基为E或Q;
(bf)在位置97上,氨基酸残基为I;
(bg)在位置100上,氨基酸残基为K或N;
(bh)在位置101上,氨基酸残基为K或R;
(bi)在位置103上,氨基酸残基为A或V;
(bj)在位置104上,氨基酸残基为D;
(bk)在位置105上,氨基酸残基为M、L或I;
(bl)在位置106上,氨基酸残基为L;
(bm)在位置112上,氨基酸残基为T或A;
(bn)在位置113上,氨基酸残基为S或T;
(bo)在位置114上,氨基酸残基为A;
(bp)在位置115上,氨基酸残基为S;
(bq)在位置119上,氨基酸残基为K或R;
(br)在位置120上,氨基酸残基为K或R;
(bs)在位置123上,氨基酸残基为F或L;
(bt)在位置125上,氨基酸残基为E;
(bu)在位置126上,氨基酸残基为Q或H;
(bv)在位置128上,氨基酸残基为E或D;
(bw)在位置129上,氨基酸残基为V或I;
(bx)在位置130上,氨基酸残基为F;
(by)在位置131上,氨基酸残基为D或E;
(bz)在位置132上,氨基酸残基为T;
(ca)在位置135上,氨基酸残基为V;
(cb)在位置138上,氨基酸残基为H;
(cc)在位置139上,氨基酸残基为I;
(cd)在位置140上,氨基酸残基为L或M;
(ce)在位置142上,氨基酸残基为Y;
(cf)在位置143上,氨基酸残基为K或R;
(cg)在位置145上,氨基酸残基为L或I;和
(ch)在位置146上,氨基酸残基为T。
80.权利要求79的分离的或重组的多肽,其中氨基酸序列中对应于在(a)-(ch)中指定的位置的氨基酸残基至少90%地遵从在(a)-(ch)中指定的氨基酸残基的限制。
81.权利要求70的分离的或重组的多肽,其中氨基酸序列中对应于如下位置的氨基酸残基至少80%地遵从如下限制:
(a)在位置9、76、94和110上,氨基酸残基为A;
(b)在位置29和108上,氨基酸残基为C;
(c)在位置34上,氨基酸残基为D;
(d)在位置95上,氨基酸残基为E;
(e)在位置56上,氨基酸残基为F;
(f)在位置43、44、66、74、87、102、116、122、127和136上,氨基酸残基为G;
(g)在位置41上,氨基酸残基为H;
(h)在位置7上,氨基酸残基为I;
(i)在位置85上,氨基酸残基为K;
(j)在位置20、42、50、78和121上,氨基酸残基为L;
(k)在位置1和141上,氨基酸残基为M;
(l)在位置23和109上,氨基酸残基为N;
(m)在位置22、25、133、134和137上,氨基酸残基为P;
(n)在位置71上,氨基酸残基为Q;
(o)在位置16、21、73、99和111上,氨基酸残基为R;
(p)在位置55上,氨基酸残基为S;
(q)在位置77上,氨基酸残基为T;
(r)在位置107上,氨基酸残基为W;和
(s)在位置13、46、70和118上,氨基酸残基为Y。
82.权利要求70-81的任何一项的分离的或重组的多肽,其中氨基酸序列包含选自由如下组成的组的氨基酸残基:
(a)在位置36上,氨基酸残基为M、L或T;
(b)在位置72上,氨基酸残基为L或I;
(c)在位置75上,氨基酸残基为M或V;
(d)在位置64上,氨基酸残基为L、I或F;
(e)在位置88上,氨基酸残基为T或S;和
(f)在位置117上,氨基酸残基为Y或F。
83.权利要求70-83的任何一项的分离的或重组的多肽,其中氨基酸序列包含一个或多个选自由如下组成的组的氨基酸残基:
(a)在位置14上,氨基酸残基为D;
(b)在位置18上,氨基酸残基为E;
(c)在位置26上,氨基酸残基为M或V;
(e)在位置30上,氨基酸残基为I;
(f)在位置32上,氨基酸残基为D;
(g)在位置36上,氨基酸残基为M或T;
(i)在位置37上,氨基酸残基为C;
(j)在位置38上,氨基酸残基为D;
(j)在位置53上,氨基酸残基为V;
(k)在位置58上,氨基酸残基为R;
(l)在位置61上,氨基酸残基为R;
(m)在位置62上,氨基酸残基为L;
(n)在位置64上,氨基酸残基为I或F;
(o)在位置65上,氨基酸残基为P;
(p)在位置72上,氨基酸残基为I;
(q)在位置75上,氨基酸残基为V;
(r)在位置88上,氨基酸残基为T;
(s)在位置89上,氨基酸残基为G;
(t)在位置91上,氨基酸残基为L;
(u)在位置98上,氨基酸残基为I;
(v)在位置105上,氨基酸残基为I;
(w)在位置112上,氨基酸残基为A;
(x)在位置124上,氨基酸残基为G或C;
(y)在位置128上,氨基酸残基为D;
(z)在位置140上,氨基酸残基为M;
(aa)在位置143上,氨基酸残基为R;和
(ab)在位置144上,氨基酸残基为W。
84.权利要求84的分离的或重组的多肽,其中氨基酸序列中对应于在(a)-(ab)中指定的位置的氨基酸残基至少80%地遵从在(a)-(ab)中指定的氨基酸残基的限制。
85.权利要求70-84的任何一项的分离的或重组的多肽,其中氨基酸序列包含一个或多个选自由如下组成的组的氨基酸残基:
(a)在位置41上,氨基酸残基为H;
(b)在位置138上,氨基酸残基为H;
(c)在位置34上,氨基酸残基为N;和
(f)在位置55上,氨基酸残基为S。
86.一种分离的或重组的多肽,包含一种选自由如下组成的组的氨基酸序列:
(a)与SEQ ID NO:577至少98%相同的氨基酸序列;
(b)与SEQ ID NO:578至少97%相同的氨基酸序列;
(c)与SEQ ID NO:621至少97%相同的氨基酸序列;
(d)与SEQ ID NO:579至少98%相同的氨基酸序列;
(e)与SEQ ID NO:602至少98%相同的氨基酸序列;
(f)与SEQ ID NO:697至少95%相同的氨基酸序列;
(g)与SEQ ID NO:712至少96%相同的氨基酸序列;
(h)与SEQ ID NO:613至少97%相同的氨基酸序列;
(i)与SEQ ID NO:677至少89%相同的氨基酸序列;
(j)与SEQ ID NO:584至少95%相同的氨基酸序列;
(k)与SEQ ID NO:707至少98%相同的氨基酸序列;
(l)与SEQ ID NO:616至少98%相同的氨基酸序列;
(m)与SEQ ID NO:612至少96%相同的氨基酸序列;
(n)与SEQ ID NO:590至少98%相同的氨基酸序列;
(o)与(a)-(n)的任何一种互补的氨基酸序列。
87.权利要求86的分离的或重组的多肽,其中氨基酸序列包含一个或多个选自由如下组成的组的氨基酸残基:
(i)在位置18和38上,为Z5氨基酸残基;
(ii)在位置62上,为Z1氨基酸残基;
(iii)在位置124上,为Z6氨基酸残基;和
(iv)在位置144上,为Z2氨基酸残基,其中:
Z1为选自由A、I、L、M和V组成的组的氨基酸残基;
Z2为选自由F、W、和Y组成的组的氨基酸残基;
Z5为选自由D和E组成的组的氨基酸残基;
Z6为选自由C、G和P组成的组的氨基酸残基;或者
88.权利要求86的分离的或重组的多肽,其中氨基酸序列中对应于如下位置的氨基酸残基至少90%地遵从如下限制:
(a)在位置2、4、15、19、26、28、31、45、51、54、86、90、91、97、103、105、106、114、123、129、139和/或145上,氨基酸残基为B1;和
(b)在位置3、5、8、10、11、14、17、24、27、32、37、47、48、49、52、57、58、61、63、68、69、79、80、82、83、89、92、100、101、104、119、120、125、126、128、131和/或143上,氨基酸残基为B2;
其中B1为选自由A、I、L、M、F、W、Y和V组成的组的氨基酸;而B2是选自由R、N、D、C、Q、E、G、H、K、P、S和T组成的组的氨基酸。
89.权利要求86的分离的或重组的多肽,其中氨基酸序列中对应于如下位置的氨基酸残基至少80%地遵从如下限制:
(a)在位置2、4、15、19、26、28、51、54、86、90、91、97、103、105、106、114、129、139和/或145上,氨基酸残基为Z1;
(b)在位置31和/或45上,氨基酸残基为Z2;
(c)在位置8上,氨基酸残基为Z3;
(d)在位置89上,氨基酸残基为Z3或者Z6;
(e)在位置82、92、101和/或120上,氨基酸残基为Z4;
(f)在位置3、11、27和/或79上,氨基酸残基为Z5;
(g)在位置18上,氨基酸残基为Z4或Z5;
(h)在位置123上,氨基酸残基为Z1或Z2;
(i)在位置12、33、35、39、53、59、112、132、135、140和/或146上,氨基酸残基为Z1或Z3;
(j)在位置30上,氨基酸残基为Z1;
(k)在位置6上,氨基酸残基为Z6;
(l)在位置81上,氨基酸残基为Z2或Z4;
(m)在位置113上,氨基酸残基为Z3;
(n)在位置138上,氨基酸残基为Z4;
(o)在位置142上,氨基酸残基为Z2;
(p)在位置57和/或126上,氨基酸残基为Z3或Z4;
(q)在位置5、17和61上,氨基酸残基为Z4;
(r)在位置24上,氨基酸残基为Z3;
(s)在位置104上,氨基酸残基为Z5;
(t)在位置52和/或69上,氨基酸残基为Z3;
(u)在位置14和/或119上,氨基酸残基为Z5;
(v)在位置10、32、63和/或83上,氨基酸残基为Z5;
(w)在位置48和/或80上,氨基酸残基为Z6;
(x)在位置40上,氨基酸残基为Z1或Z2;
(y)在位置96上,氨基酸残基为Z3或Z5;
(z)在位置65上,氨基酸残基为Z3、Z4或Z6;
(aa)在位置84和/或115上,氨基酸残基为Z3;
(ab)在位置93上,氨基酸残基为Z4;
(ac)在位置130上,氨基酸残基为Z2;
(ad)在位置58上,氨基酸残基为Z3、Z4或者Z6;
(ae)在位置47上,氨基酸残基为Z4或Z6;
(af)在位置49和/或100上,氨基酸残基为Z3或Z4;
(ag)在位置68上,氨基酸残基为Z4或Z5;
(ah)在位置143上,氨基酸残基为Z4;
(ai)在位置131上,氨基酸残基为Z5;
(aj)在位置125和/或128上,氨基酸残基为Z5;
(ak)在位置67上,氨基酸残基为Z3或Z4;
(al)在位置60上,氨基酸残基为Z5;和
(am)在位置37上,氨基酸残基为Z4或Z6;
其中Z1是选自由A、I、L、M和V组成的组的氨基酸残基;Z2是选自由F、W和Y组成的组的氨基酸残基;Z3是选自由N、Q、S和T组成的组的氨基酸;Z4是选自由R、H和K组成的组的氨基酸;Z5是选自由D和E组成的组的氨基酸残基;和Z6是选自由C、G和P组成的组的氨基酸残基。
90.权利要求89的分离的或重组的多肽,其中氨基酸序列中对应于在(a)-(am)中指定的位置的氨基酸残基至少90%地遵从在(a)-(am)中指定的氨基酸残基的限制。
91.权利要求86-90的任何一项的分离的或重组的多肽,其中氨基酸序列中对应于如下位置的氨基酸残基至少90%地遵从如下限制:
(a)在位置1、7、9、13、20、36、42、46、50、56、64、70、72、75、76、78、94、98、107、110、117、118、121和/或141上,氨基酸残基为B1;和
(b)在位置16、21、22、23、25、29、34、41、43、44、55、66、71、73、74、77、85、87、88、95、99、102、108、109、111、116、122、127、133、134、136和/或137上,氨基酸残基为B2;
其中B1为选自由A、I、L、M、F、W、Y和V组成的组的氨基酸;而B2是选自由R、N、D、C、Q、E、G、H、K、P、S和T组成的组的氨基酸。
92.权利要求86-91的任何一项的分离的或重组的多肽,其中氨基酸序列中对应于如下位置的氨基酸残基至少90%地遵从如下限制:
(a)在位置1、7、9、13、20、42、46、50、56、64、70、72、75、76、78、94、98、107、110、117、118、121和/或141上,氨基酸残基为B1;和
(b)在位置16、21、22、23、25、29、34、36、41、43、44、55、66、71、73、74、77、85、87、88、95、99、102、108、109、111、116、122、127、133、134、136和/或137上,氨基酸残基为B2;
其中B1为选自由A、I、L、M、F、W、Y和V组成的组的氨基酸;而B2是选自由R、N、D、C、Q、E、G、H、K、P、S和T组成的组的氨基酸。
93.权利要求86-91的任何一项的分离的或重组的多肽,其中氨基酸序列中对应于如下位置的氨基酸残基至少90%地遵从如下限制:
(a)在位置1、7、9、20、42、50、72、75、76、78、94、98、110、121和/或141上,氨基酸残基为Z1;
(b)在位置13、46、56、70、107、117和/或118上,氨基酸残基为Z2;
(c)在位置23、55、71、77、88和/或109上,氨基酸残基为Z 3;
(d)在位置16、21、41、73、85、99和/或111上,氨基酸残基为Z4;
(e)在位置34和/或95上,氨基酸残基为Z5;
(f)在位置22,25,29,43,44、66、74、87、102、108、116、122、127、133、134、136和/或137上,氨基酸残基为Z6;
其中Z1是选自由A、I、L、M和V组成的组的氨基酸残基;Z2是选自由F、W和Y组成的组的氨基酸残基;Z3是选自由N、Q、S和T组成的组的氨基酸;Z4是选自由R、H和K组成的组的氨基酸;Z5是选自由D和E组成的组的氨基酸残基;和Z6是选自由C、G和P组成的组的氨基酸残基。
94.权利要求93的分离的或重组的多肽,其中氨基酸序列在位置36上包含选自由Z1和Z3组成的组的氨基酸残基。
95.权利要求93或94的分离的或重组的多肽,其中氨基酸序列在位置64上还包含选自由Z1和Z2组成的组的氨基酸残基。
96.权利要求86-95的任何一项的分离的或重组的多肽,其中氨基酸序列中对应于如下位置的氨基酸残基至少80%地遵从如下限制:
(a)在位置2上,氨基酸残基为I或L;
(b)在位置3上,氨基酸残基为E;
(c)在位置4上,氨基酸残基为V或I;
(d)在位置5上,氨基酸残基为K;
(e)在位置6上,氨基酸残基为P;
(f)在位置8上,氨基酸残基为N;
(g)在位置10上,氨基酸残基为E;
(h)在位置11上,氨基酸残基为D或E;
(i)在位置12上,氨基酸残基为T;
(j)在位置14上,氨基酸残基为E或D;
(k)在位置15上,氨基酸残基为L;
(l)在位置17上,氨基酸残基为H;
(m)在位置18上,氨基酸残基为R、E或K;
(n)在位置19上,氨基酸残基为I或V;
(o)在位置24上,氨基酸残基为Q;
(p)在位置26上,氨基酸残基为M、L、V或I;
(q)在位置27上,氨基酸残基为E;
(r)在位置28上,氨基酸残基为A或V;
(s)在位置30上,氨基酸残基为M;
(t)在位置31上,氨基酸残基为Y或F;
(u)在位置32上,氨基酸残基为E或D;
(v)在位置33上,氨基酸残基为T或S;
(w)在位置35上,氨基酸残基为L;
(x)在位置37上,氨基酸残基为R、G、E或Q;
(y)在位置39上,氨基酸残基为A或S;
(z)在位置40上,氨基酸残基为F或L;
(aa)在位置45上,氨基酸残基为Y或F;
(ab)在位置47上,氨基酸残基为R或G;
(ac)在位置48上,氨基酸残基为G;
(ad)在位置49上,氨基酸残基为K、R或Q;
(ae)在位置51上,氨基酸残基为I或V;
(af)在位置52上,氨基酸残基为S;
(ag)在位置53上,氨基酸残基为I或V;
(ah)在位置54上,氨基酸残基为A;
(ai)在位置57上,氨基酸残基为H或N;
(aj)在位置58上,氨基酸残基为Q、K、R或P;
(ak)在位置59上,氨基酸残基为A;
(al)在位置60上,氨基酸残基为E;
(am)在位置61上,氨基酸残基为H或R;
(an)在位置63上,氨基酸残基为E或D;
(ao)在位置65上,氨基酸残基为E、P或Q;
(ap)在位置67上,氨基酸残基为Q或R;
(aq)在位置68上,氨基酸残基为K或E;
(ar)在位置69上,氨基酸残基为Q;
(as)在位置79上,氨基酸残基为E;
(at)在位置80上,氨基酸残基为G;
(au)在位置81上,氨基酸残基为Y、H或F;
(av)在位置82上,氨基酸残基为R;
(aw)在位置83上,氨基酸残基为E或D;
(ax)在位置84上,氨基酸残基为Q;
(ay)在位置86上,氨基酸残基为A;
(az)在位置89上,氨基酸残基为G、T或S;
(ba)在位置90上,氨基酸残基为L;
(bb)在位置91上,氨基酸残基为L、I或V;
(bc)在位置92上,氨基酸残基为R或K;
(bd)在位置93上,氨基酸残基为H;
(be)在位置96上,氨基酸残基为E或Q;
(bf)在位置97上,氨基酸残基为I;
(bg)在位置100上,氨基酸残基为K或N;
(bh)在位置101上,氨基酸残基为K或R;
(bi)在位置103上,氨基酸残基为A或V;
(bj)在位置104上,氨基酸残基为D;
(bk)在位置105上,氨基酸残基为M、L或I;
(bl)在位置106上,氨基酸残基为L;
(bm)在位置112上,氨基酸残基为T或A;
(bn)在位置113上,氨基酸残基为S或T;
(bo)在位置114上,氨基酸残基为A;
(bp)在位置115上,氨基酸残基为S;
(bq)在位置119上,氨基酸残基为K或R;
(br)在位置120上,氨基酸残基为K或R;
(bs)在位置123上,氨基酸残基为F或L;
(bt)在位置125上,氨基酸残基为E;
(bu)在位置126上,氨基酸残基为Q或H;
(bv)在位置128上,氨基酸残基为E或D;
(bw)在位置129上,氨基酸残基为V或I;
(bx)在位置130上,氨基酸残基为F;
(by)在位置131上,氨基酸残基为D或E;
(bz)在位置132上,氨基酸残基为T;
(ca)在位置135上,氨基酸残基为V;
(cb)在位置138上,氨基酸残基为H;
(cc)在位置139上,氨基酸残基为I;
(cd)在位置140上,氨基酸残基为L或M;
(ce)在位置142上,氨基酸残基为Y;
(cf)在位置143上,氨基酸残基为K或R;
(cg)在位置145上,氨基酸残基为L或I;和
(ch)在位置146上,氨基酸残基为T。
97.权利要求96的分离的或重组的多肽,其中氨基酸序列中对应于在(a)-(ch)中指定的位置的氨基酸残基至少90%地遵从在(a)-(ch)中指定的氨基酸残基的限制。
98.权利要求86的分离的或重组的多肽,其中氨基酸序列中对应于如下位置的氨基酸残基至少80%地遵从如下限制:
(a)在位置9、76、94和110上,氨基酸残基为A;
(b)在位置29和108上,氨基酸残基为C;
(c)在位置34上,氨基酸残基为D;
(d)在位置95上,氨基酸残基为E;
(e)在位置56上,氨基酸残基为F;
(f)在位置43、44、66、74、87、102、116、122、127和136上,氨基酸残基为G;
(g)在位置41上,氨基酸残基为H;
(h)在位置7上,氨基酸残基为I;
(i)在位置85上,氨基酸残基为K;
(j)在位置20、42、50、78和121上,氨基酸残基为L;
(k)在位置1和141上,氨基酸残基为M;
(l)在位置23和109上,氨基酸残基为N;
(m)在位置22、25、133、134和137上,氨基酸残基为P;
(n)在位置71上,氨基酸残基为Q;
(o)在位置16、21、73、99和111上,氨基酸残基为R;
(p)在位置55上,氨基酸残基为S;
(q)在位置77上,氨基酸残基为T;
(r)在位置107上,氨基酸残基为W;和
(s)在位置13、46、70和118上,氨基酸残基为Y。
99.权利要求70-98的任何一项的分离的或重组的多肽,其中氨基酸序列包含一个或多个选自由如下组成的组的氨基酸残基:
(a)在位置14上,氨基酸残基为D;
(b)在位置18上,氨基酸残基为E;
(c)在位置26上,氨基酸残基为M或V;
(e)在位置30上,氨基酸残基为I;
(f)在位置32上,氨基酸残基为D;
(g)在位置36上,氨基酸残基为M或T;
(i)在位置37上,氨基酸残基为C;
(j)在位置38上,氨基酸残基为D;
(j)在位置53上,氨基酸残基为V;
(k)在位置58上,氨基酸残基为R;
(l)在位置61上,氨基酸残基为R;
(m)在位置62上,氨基酸残基为L;
(n)在位置64上,氨基酸残基为I或F;
(o)在位置65上,氨基酸残基为P;
(p)在位置72上,氨基酸残基为I;
(q)在位置75上,氨基酸残基为V;
(r)在位置88上,氨基酸残基为T;
(s)在位置89上,氨基酸残基为G;
(t)在位置91上,氨基酸残基为L;
(u)在位置98上,氨基酸残基为I;
(v)在位置105上,氨基酸残基为I;
(w)在位置112上,氨基酸残基为A;
(x)在位置124上,氨基酸残基为G或C;
(y)在位置128上,氨基酸残基为D;
(z)在位置140上,氨基酸残基为M;
(aa)在位置143上,氨基酸残基为R;和
(ab)在位置144上,氨基酸残基为W。
100.权利要求99的分离的或重组的多肽,其中氨基酸序列中对应于在(a)-(ab)中指定的位置的氨基酸残基至少80%地遵从在(a)-(ab)中指定的氨基酸残基的限制。
101权利要求86-100的任何一项的分离的或重组的多肽,其中氨基酸序列包含一个或多个选自由如下组成的组的氨基酸残基:
(a)在位置41上,氨基酸残基为H;
(b)在位置138上,氨基酸残基为H;
(c)在位置34上,氨基酸残基为N;和
(e)在位置55上,氨基酸残基为S。
102.权利要求69的分离的或重组的多肽,其中该多肽以对于草甘膦至少为10nM-1的kcat/Km催化草甘膦的乙酰化。
103.权利要求102的分离的或重组的多肽,其中该多肽以对于草甘膦至少为100mM-1的k催化/Km催化草甘膦的乙酰化。
104.权利要求103的分离的或重组的多肽,其中该多肽催化氨基甲基膦酸的乙酰化。
105.一种具有GAT活性的多肽,所述多肽选自由如下组成的组:SEQ ID NO:568、569、570、571、572、573、574、575、576、577、578、579、580、581、582、583、584、585、586、587、588、589、590、591、592、593、594、595、596、597、598、599、600、601、602、603、604、605、606、607、608、609、610、611、612、613、614、615、616、617、618、619、621、623、625、627、629、631、633、635、637、639、641、643、645、647、649、651、653、655、657、659、661、663、665、667、669、671、673、675、677、679、681、683、685、687、689、691、693、695、697、699、701、703、705、707、709、711、713、715、717、719、721、723、725、727、729、731、733、735、737、739、741、743、745、747、749、751、753、755、757、759、761、763、765、767、769、771、773、775、777、779、781、783、785、787、789、791、793、795、797、799、801、803、805、807、809、811、813、815、817、819、821、823、825、833、835、837、839、841、843、845、847、849、851、853、855、857、859、861、863、865、867、869、871、873、875、877、879、881、883、885、887、889、891、893、895、897、899、901、903、905、907、909、911、913、915、917、919、921、923、925、927、929、931、946、948、950、953、954、955、956、957、958、959、960、961、962、963、964、965、966、967、968、969、970、971和972,其特征在于:
(a)对于草甘膦的Km至少为约2mM或更小;
(b)对于乙酰CoA的Km至少为约200μM或更小;和
(c)k催化等于至少约6/分钟。
106.一种具有草甘膦-N-乙酰转移酶活性的分离的或重组的多肽,所述多肽选自由如下组成的组:SEQ ID NO:568、569、570、571、572、573、574、575、576、577、578、579、580、581、582、583、584、585、586、587、588、589、590、591、592、593、594、595、596、597、598、599、600、601、602、603、604、605、606、607、608、609、610、611、612、613、614、615、616、617、618、619、621、623、625、627、629、631、633、635、637、639、641、643、645、647、649、651、653、655、657、659、661、663、665、667、669、671、673、675、677、679、681、683、685、687、689、691、693、695、697、699、701、703、705、707、709、711、713、715、717、719、721、723、725、727、729、731、733、735、737、739、741、743、745、747、749、751、753、755、757、759、761、763、765、767、769、771、773、775、777、779、781、783、785、787、789、791、793、795、797、799、801、803、805、807、809、811、813、815、817、819、821、823、825、833、835、837、839、841、843、845、847、849、851、853、855、857、859、861、863、865、867、869、871、873、875、877、879、881、883、885、887、889、891、893、895、897、899、901、903、905、907、909、911、913、915、917、919、921、923、925、927、929、931、946、948、950、953、954、955、956、957、958、959、960、961、962、963、964、965、966、967、968、969、970、971和972。
107.一种用于生产草甘膦抗性的转基因植物或植物细胞的方法,包括:
(a)用编码草甘膦-N-乙酰转移酶的多核苷酸转化植物或植物细胞,所述多核苷酸选自由如下组成的组:SEQ ID NO:516、517、518、519、520、521、522、523、524、525、526、527、528、529、530、531、532、533、534、535、536、537、538、539、540、541、542、543、544、545、546、547、548、549、550、551、552、553、554、555、556、557、558、559、560、561、562、563、564、565、566、567、620、622、624、626、628、630、632、634、636、638、640、642、644、646、648、650、652、654、656、658、660、662、664、666、668、670、672、674、676、678、680、682、684、686、688、690、692、694、696、698、700、702、704、706、708、710、712、714、716、718、720、722、724、726、728、730、732、734、736、738、740、742、744、746、748、750、752、754、756、758、760、762、764、768、770、772、774、776、778、780、782、784、786、788、790、792、794、796、798、800、802、804、806、808、810、812、814、816、818、820、822、824、832、834、836、838、840、842、844、846、848、850、852、854、856、858、860、862、864、866、868、870、872、874、876、878、880、882、884、886、888、890、892、894、896、898、900、902、904、906、908、910、912、914、916、918、920、922、924、926、928、930、932、933、934、935、936、937、938、939、940、941、942、943、944、945、947、949、951和952;和
(b)任选地由被转化的植物细胞再生转基因植物。
108.一种用于选择含有核酸构建体的植物或植物细胞的方法,包括:
(a)提供包含核酸构建体的转基因植物或植物细胞,其中该核酸构建体包含编码草甘膦-N-乙酰转移酶的核苷酸序列,所述核苷酸序列选自由如下组成的组:SEQ ID NO:516、517、518、519、520、521、522、523、524、525、526、527、528、529、530、531、532、533、534、535、536、537、538、539、540、541、542、543、544、545、546、547、548、549、550、551、552、553、554、555、556、557、558、559、560、561、562、563、564、565、566、567、620、622、624、626、628、630、632、634、636、638、640、642、644、646、648、650、652、654、656、658、660、662、664、666、668、670、672、674、676、678、680、682、684、686、688、690、692、694、696、698、700、702、704、706、708、710、712、714、716、718、720、722、724、726、728、730、732、734、736、738、740、742、744、746、748、750、752、754、756、758、760、762、764、768、770、772、774、776、778、780、782、784、786、788、790、792、794、796、798、800、802、804、806、808、810、812、814、816、818、820、822、824、832、834、836、838、840、842、844、846、848、850、852、854、856、858、860、862、864、866、868、870、872、874、876、878、880、882、884、886、888、890、892、894、896、898、900、902、904、906、908、910、912、914、916、918、920、922、924、926、928、930、932、933、934、935、936、937、938、939、940、941、942、943、944、945、947、949、951和952;和
(b)在草甘膦的存在下,使转基因植物或植物细胞在草甘膦-N-乙酰转移酶以有效水平被表达的条件下生长,其中转基因植物或植物细胞的生长速度明显比不含核酸构建体的植物或细胞的生长速度快。
109.一种用于选择性地控制具有农作物的田间的杂草的方法,包括:
(a)将用编码草甘膦N-乙酰转移酶的基因转化而具有草甘膦抗性的农作物种子或植物种植在田间,所述基因选自由如下组成的组SEQID NO:516、517、518、519、520、521、522、523、524、525、526、527、528、529、530、531、532、533、534、535、536、537、538、539、540、541、542、543、544、545、546、547、548、549、550、551、552、553、554、555、556、557、558、559、560、561、562、563、564、565、566、567、620、622、624、626、628、630、632、634、636、638、640、642、644、646、648、650、652、654、656、658、660、662、664、666、668、670、672、674、676、678、680、682、684、686、688、690、692、694、696、698、700、702、704、706、708、710、712、714、716、718、720、722、724、726、728、730、732、734、736、738、740、742、744、746、748、750、752、754、756、758、760、762、764、768、770、772、774、776、778、780、782、784、786、788、790、792、794、796、798、800、802、804、806、808、810、812、814、816、818、820、822、824、832、834、836、838、840、842、844、846、848、850、852、854、856、858、860、862、864、866、868、870、872、874、876、878、880、882、884、886、888、890、892、894、896、898、900、902、904、906、908、910、912、914、916、918、920、922、924、926、928、930、932、933、934、935、936、937、938、939、940、941、942、943、944、945、947、949、951和952;和
(b)对田间的农作物和杂草施用足量的草甘膦,以控制杂草而不显著地影响农作物。
110.权利要求109的方法,其中农作物是棉花、玉米或大豆。
111.一种具有增强的对草甘膦的耐受性的转基因植物或转基因植物外植体,其中该植物或植物外植体表达一种具有草甘膦N-乙酰转移酶活性的多肽,所述多肽选自由如下组成的组:SEQ ID NO:568、569、570、571、572、573、574、575、576、577、578、579、580、581、582、583、584、585、586、587、588、589、590、591、592、593、594、595、596、597、598、599、600、601、602、603、604、605、606、607、608、609、610、611、612、613、614、615、616、617、618、619、621、623、625、627、629、631、633、635、637、639、641、643、645、647、649、651、653、655、657、659、661、663、665、667、669、671、673、675、677、679、681、683、685、687、689、691、693、695、697、699、701、703、705、707、709、711、713、715、717、719、721、723、725、727、729、731、733、735、737、739、741、743、745、747、749、751、753、755、757、759、761、763、765、767、769、771、773、775、777、779、781、783、785、787、789、791、793、795、797、799、801、803、805、807、809、811、813、815、817、819、821、823、825、833、835、837、839、841、843、845、847、849、851、853、855、857、859、861、863、865、867、869、871、873、875、877、879、881、883、885、887、889、891、893、895、897、899、901、903、905、907、909、911、913、915、917、919、921、923、925、927、929、931、946、948、950、953、954、955、956、957、958、959、960、961、962、963、964、965、966、967、968、969、970、971和972;并且至少一种多肽通过其他机制赋予对草甘膦的耐受性。
112.一种转基因植物或转基因植物外植体,其中该植物或植物外植体表达一种具有草甘膦N-乙酰转移酶活性的多肽,所述多肽选自由如下组成的组:SEQ ID NO:568、569、570、571、572、573、574、575、576、577、578、579、580、581、582、583、584、585、586、587、588、589、590、591、592、593、594、595、596、597、598、599、600、601、602、603、604、605、606、607、608、609、610、611、612、613、614、615、616、617、618、619、621、623、625、627、629、631、633、635、637、639、641、643、645、647、649、651、653、655、657、659、661、663、665、667、669、671、673、675、677、679、681、683、685、687、689、691、693、695、697、699、701、703、705、707、709、711、713、715、717、719、721、723、725、727、729、731、733、735、737、739、741、743、745、747、749、751、753、755、757、759、761、763、765、767、769、771、773、775、777、779、781、783、785、787、789、791、793、795、797、799、801、803、805、807、809、811、813、815、817、819、821、823、825、833、835、837、839、841、843、845、847、849、851、853、855、857、859、861、863、865、867、869、871、873、875、877、879、881、883、885、887、889、891、893、895、897、899、901、903、905、907、909、911、913、915、917、919、921、923、925、927、929、931、946、948、950、953、954、955、956、957、958、959、960、961、962、963、964、965、966、967、968、969、970、971和972;并且至少一种多肽赋予对其他除草剂的耐受性。
113.一种具有增强的对草甘膦的耐受性的转基因植物或转基因植物外植体,其中该植物或植物外植体表达一种具有草甘膦N-乙酰转移酶活性的多肽,所述多肽选自由如下组成的组:SEQ ID NO:568、569、570、571、572、573、574、575、576、577、578、579、580、581、582、583、584、585、586、587、588、589、590、591、592、593、594、595、596、597、598、599、600、601、602、603、604、605、606、607、608、609、610、611、612、613、614、615、616、617、618、619、621、623、625、627、629、631、633、635、637、639、641、643、645、647、649、651、653、655、657、659、661、663、665、667、669、671、673、675、677、679、681、683、685、687、689、691、693、695、697、699、701、703、705、707、709、711、713、715、717、719、721、723、725、727、729、731、733、735、737、739、741、743、745、747、749、751、753、755、757、759、761、763、765、767、769、771、773、775、777、779、781、783、785、787、789、791、793、795、797、799、801、803、805、807、809、811、813、815、817、819、821、823、825、833、835、837、839、841、843、845、847、849、851、853、855、857、859、861、863、865、867、869、871、873、875、877、879、881、883、885、887、889、891、893、895、897、899、901、903、905、907、909、911、913、915、917、919、921、923、925、927、929、931、946、948、950、953、954、955、956、957、958、959、960、961、962、963、964、965、966、967、968、969、970、971和972;至少一种多肽通过其他机制赋予对草甘膦的耐受性并且至少一种多肽赋予对其他除草剂的耐受性。
114.一种用于控制具有农作物的田间的杂草的方法,包括:
(a)在田间种植用编码草甘膦N-乙酰转移酶的基因转化的农作物种子或植物,所述基因选自由如下组成的组:SEQ ID NO:516、517、518、519、520、521、522、523、524、525、526、527、528、529、530、531、532、533、534、535、536、537、538、539、540、541、542、543、544、545、546、547、548、549、550、551、552、553、554、555、556、557、558、559、560、561、562、563、564、565、566、567、620、622、624、626、628、630、632、634、636、638、640、642、644、646、648、650、652、654、656、658、660、662、664、666、668、670、672、674、676、678、680、682、684、686、688、690、692、694、696、698、700、702、704、706、708、710、712、714、716、718、720、722、724、726、728、730、732、734、736、738、740、742、744、746、748、750、752、754、756、758、760、762、764、768、770、772、774、776、778、780、782、784、786、788、790、792、794、796、798、800、802、804、806、808、810、812、814、816、818、820、822、824、832、834、836、838、840、842、844、846、848、850、852、854、856、858、860、862、864、866、868、870、872、874、876、878、880、882、884、886、888、890、892、894、896、898、900、902、904、906、908、910、912、914、916、918、920、922、924、926、928、930、932、933、934、935、936、937、938、939、940、941、942、943、944、945、947、949、951和952;并且至少一种基因编码通过其他机制赋予草甘膦耐受性的多肽;和
(b)给田间的农作物和杂草施用足以抑制田间的杂草生长而不显著地影响该农作物的草甘膦。
115.一种用于选择性地控制具有农作物的田间的杂草的方法,包括:
(a)在田间种植用编码草甘膦N-乙酰转移酶的基因转化的农作物种子或植物,所述基因选自由如下组成的组:SEQ ID NO:516、517、518、519、520、521、522、523、524、525、526、527、528、529、530、531、532、533、534、535、536、537、538、539、540、541、542、543、544、545、546、547、548、549、550、551、552、553、554、555、556、557、558、559、560、561、562、563、564、565、566、567、620、622、624、626、628、630、632、634、636、638、640、642、644、646、648、650、652、654、656、658、660、662、664、666、668、670、672、674、676、678、680、682、684、686、688、690、692、694、696、698、700、702、704、706、708、710、712、714、716、718、720、722、724、726、728、730、732、734、736、738、740、742、744、746、748、750、752、754、756、758、760、762、764、768、770、772、774、776、778、780、782、784、786、788、790、792、794、796、798、800、802、804、806、808、810、812、814、816、818、820、822、824、832、834、836、838、840、842、844、846、848、850、852、854、856、858、860、862、864、866、868、870、872、874、876、878、880、882、884、886、888、890、892、894、896、898、900、902、904、906、908、910、912、914、916、918、920、922、924、926、928、930、932、933、934、935、936、937、938、939、940、941、942、943、944、945、947、949、951和952;并且至少一种基因编码赋予对其他除草剂的耐受性的多肽;和
(b)给田间的农作物和杂草同时或者顺序交错地施用草甘膦和其他除草剂,它们足以抑制田间的杂草生长而不显著地影响该农作物。
116.一种用于选择性地控制具有农作物的田间的杂草的方法,包括:
(a)在田间种植用编码草甘膦N-乙酰转移酶的基因转化的农作物种子或植物,所述基因选自由如下组成的组:SEQ ID NO:516、517、518、519、520、521、522、523、524、525、526、527、528、529、530、531、532、533、534、535、536、537、538、539、540、541、542、543、544、545、546、547、548、549、550、551、552、553、554、555、556、557、558、559、560、561、562、563、564、565、566、567、620、622、624、626、628、630、632、634、636、638、640、642、644、646、648、650、652、654、656、658、660、662、664、666、668、670、672、674、676、678、680、682、684、686、688、690、692、694、696、698、700、702、704、706、708、710、712、714、716、718、720、722、724、726、728、730、732、734、736、738、740、742、744、746、748、750、752、754、756、758、760、762、764、768、770、772、774、776、778、780、782、784、786、788、790、792、794、796、798、800、802、804、806、808、810、812、814、816、818、820、822、824、832、834、836、838、840、842、844、846、848、850、852、854、856、858、860、862、864、866、868、870、872、874、876、878、880、882、884、886、888、890、892、894、896、898、900、902、904、906、908、910、912、914、916、918、920、922、924、926、928、930、932、933、934、935、936、937、938、939、940、941、942、943、944、945、947、949、951和952;至少一种基因编码通过其他机制赋予对草甘膦的耐受性的多肽并且至少一种基因编码赋予对其他除草剂的耐受性的多肽;和
(b)给田间的农作物和杂草同时或者顺序交错地施用草甘膦和其他除草剂,它们足以抑制田间的杂草生长而不显著地影响该农作物。
117.一种分离的或重组的多核苷酸,包含选自由如下组成的组的核苷酸序列:
(a)编码与SEQ ID NO:919至少96%相同的氨基酸序列的核苷酸序列;
(b)编码与SEQ ID NO:929至少97%相同的氨基酸序列的核苷酸序列;
(c)编码与SEQ ID NO:847至少98%相同的氨基酸序列的核苷酸序列;
(d)编码与SEQ ID NO:851至少98%相同的氨基酸序列的核苷酸序列;
(e)编码与SEQ ID NO:853至少98%相同的氨基酸序列的核苷酸序列;
(f)编码与SEQ ID NO:855至少98%相同的氨基酸序列的核苷酸序列;
(g)编码与SEQ ID NO:857至少98%相同的氨基酸序列的核苷酸序列;
(h)编码与SEQ ID NO:861至少98%相同的氨基酸序列的核苷酸序列;
(i)编码与SEQ ID NO:871至少98%相同的氨基酸序列的核苷酸序列;
(j)编码与SEQ ID NO:875至少98%相同的氨基酸序列的核苷酸序列;
(k)编码与SEQ ID NO:881至少98%相同的氨基酸序列的核苷酸序列;
(l)编码与SEQ ID NO:885至少98%相同的氨基酸序列的核苷酸序列;
(m)编码与SEQ ID NO:887至少98%相同的氨基酸序列的核苷酸序列;
(n)编码与SEQ ID NO:889至少98%相同的氨基酸序列的核苷酸序列;
(o)编码与SEQ ID NO:893至少98%相同的氨基酸序列的核苷酸序列;
(p)编码与SEQ ID NO:897至少98%相同的氨基酸序列的核苷酸序列;
(q)编码与SEQ ID NO:899至少98%相同的氨基酸序列的核苷酸序列;
(r)编码与SEQ ID NO:909至少98%相同的氨基酸序列的核苷酸序列;
(s)编码与SEQ ID NO:911至少98%相同的氨基酸序列的核苷酸序列;
(t)编码与SEQ ID NO:837至少99%相同的氨基酸序列的核苷酸序列;
(u)编码与SEQ ID NO:841至少99%相同的氨基酸序列的核苷酸序列;
(v)编码与SEQ ID NO:865至少99%相同的氨基酸序列的核苷酸序列;
(w)编码与SEQ ID NO:869至少99%相同的氨基酸序列的核苷酸序列;和
(x)编码与SEQ ID NO:879至少99%相同的氨基酸序列的核苷酸序列。
118.一种分离的或重组的多核苷酸,它包含编码一种氨基酸序列的核苷酸序列:该氨基酸序列与SEQ ID NO:929至少95%相同,并且在对应于SEQ ID NO:929的位置33的氨基酸位置上包含Gly或者Asn残基。
119.一种编码具有草甘膦-N-乙酰转移酶活性的多肽的分离的或重组的多核苷酸,所述多核苷酸选自由如下组成的组:SEQ ID NO:832、834、836、838、840、842、844、846、848、850、852、854、856、858、860、862、864、866、868、870、872、874、876、878、880、882、884、886、888、890、892、894、896、898、900、902、904、906、908、910、912、914、916、918、920、922、924、926、928、930、932、933、934、935、936、937、938、939、940、941、942、943、944、945、947、949、951和952。
120.一种分离的或重组的多肽,包含选自由如下组成的组的氨基酸序列:
(a)与SEQ ID NO:919至少96%相同的氨基酸序列;
(b)与SEQ ID NO:929至少97%相同的氨基酸序列;
(c)与SEQ ID NO:847至少98%相同的氨基酸序列;
(d)与SEQ ID NO:851至少98%相同的氨基酸序列;
(e)与SEQ ID NO:853至少98%相同的氨基酸序列;
(f)与SEQ ID NO:855至少98%相同的氨基酸序列;
(g)与SEQ ID NO:857至少98%相同的氨基酸序列;
(h)与SEQ ID NO:861至少98%相同的氨基酸序列;
(i)与SEQ ID NO:871至少98%相同的氨基酸序列;
(j)与SEQ ID NO:875至少98%相同的氨基酸序列;
(k)与SEQ ID NO:881至少98%相同的氨基酸序列;
(l)与SEQ ID NO:885至少98%相同的氨基酸序列;
(m)与SEQ ID NO:887至少98%相同的氨基酸序列;
(n)与SEQ ID NO:889至少98%相同的氨基酸序列;
(o)与SEQ ID NO:893至少98%相同的氨基酸序列;
(p)与SEQ ID NO:897至少98%相同的氨基酸序列;
(q)与SEQ ID NO:899至少98%相同的氨基酸序列;
(r)与SEQ ID NO:909至少98%相同的氨基酸序列;
(s)与SEQ ID NO:911至少98%相同的氨基酸序列;
(t)与SEQ ID NO:837至少99%相同的氨基酸序列;
(u)与SEQ ID NO:841至少99%相同的氨基酸序列;
(v)与SEQ ID NO:865至少99%相同的氨基酸序列;
(w)与SEQ ID NO:869至少99%相同的氨基酸序列;和
(x)与SEQ ID NO:879至少99%相同的氨基酸序列。
121.一种分离的或重组的多肽,包含与SEQ ID NO:929至少95%相同的氨基酸序列,并且在对应于SEQ ID NO:929的位置33的氨基酸位置上包含Gly或者Asn残基。
122.一种分离的或重组的多肽,包含选自由如下组成的组的氨基酸序列的残基2-146:SEQ ID NO:833、835、837、839、841、843、845、847、849、851、853、855、857、859、861、863、865、867、869、871、873、875、877、879、881、883、885、887、889、891、893、895、897、899、901、903、905、907、909、911、913、915、917、919、921、923、925、927、929、931、946、948、950、953、954、955、956、957、958、959、960、961、962、963、964、965、966、967、968、969、970、971和972。
123.一种分离的或重组的多肽,包含选自由如下组成的组的氨基酸序列:SEQ ID NO:833、835、837、839、841、843、845、847、849、851、853、855、857、859、861、863、865、867、869、871、873、875、877、879、881、883、885、887、889、891、893、895、897、899、901、903、905、907、909、911、913、915、917、919、921、923、925、927、929、931、946、948、950、953、954、955、956、957、958、959、960、961、962、963、964、965、966、967、968、969、970、971和972。
124.一种用于提高植物或植物细胞中多肽的表达的方法,该方法包括:
(a)获得第一条包含编码一种多肽的核酸序列的多核苷酸,该多肽包含一种氨基酸序列,采用BLOSUM62矩阵,存在一个缺口罚11分,延伸一个缺口罚1分,将该氨基酸序列与选自由SEQ ID NO:300、SEQID NO:445和SEQ ID NO:457组成的组的序列最佳地比对,产生至少为460的相似性分值,该核酸序列包含起始甲硫氨酸ATG密码子;
(b)将一个或两个GCG或者GCT密码子插入到正好位于起始甲硫氨酸ATG密码子的下游或者邻近于起始甲硫氨酸ATG密码子的核酸序列中,产生被修饰的多核苷酸;和
(c)用被修饰的多核苷酸转化植物或植物细胞,其中相对于用第一条多核苷酸转化的植物或植物细胞,用被修饰的多核苷酸转化的植物或植物细胞积累较高含量的被编码的多肽。
125.权利要求124的方法,还包括鉴定核酸序列中的一种或多种在植物中以较小的频率被使用的密码子,用在植物中更加频繁地使用的编码相同氨基酸的密码子取代所说的在植物中以较小的频率被使用的密码子。
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---|---|---|---|---|
DK2308976T4 (en) | 2004-04-30 | 2017-07-03 | Dow Agrosciences Llc | Enjoy herbicide resistance gene |
CN104585228B (zh) | 2005-03-04 | 2017-05-24 | 孟山都技术公司 | 减轻用除草剂草甘膦制剂处理的草甘膦耐受性转基因棉花植物内的坏死 |
WO2007024782A2 (en) | 2005-08-24 | 2007-03-01 | Pioneer Hi-Bred International, Inc. | Compositions providing tolerance to multiple herbicides and methods of use thereof |
US7951995B2 (en) | 2006-06-28 | 2011-05-31 | Pioneer Hi-Bred International, Inc. | Soybean event 3560.4.3.5 and compositions and methods for the identification and detection thereof |
US7968770B2 (en) | 2006-06-28 | 2011-06-28 | Pioneer Hi-Bred International, Inc. | Methods for improving yield using soybean event 3560.4.3.5 |
US7897846B2 (en) | 2006-10-30 | 2011-03-01 | Pioneer Hi-Bred Int'l, Inc. | Maize event DP-098140-6 and compositions and methods for the identification and/or detection thereof |
US7928296B2 (en) | 2006-10-30 | 2011-04-19 | Pioneer Hi-Bred International, Inc. | Maize event DP-098140-6 and compositions and methods for the identification and/or detection thereof |
CL2007003743A1 (es) | 2006-12-22 | 2008-07-11 | Bayer Cropscience Ag | Composicion que comprende fenamidona y un compuesto insecticida; y metodo para controlar de forma curativa o preventiva hongos fitopatogenos de cultivos e insectos. |
CL2007003744A1 (es) | 2006-12-22 | 2008-07-11 | Bayer Cropscience Ag | Composicion que comprende un derivado 2-piridilmetilbenzamida y un compuesto insecticida; y metodo para controlar de forma curativa o preventiva hongos fitopatogenos de cultivos e insectos. |
EP1969931A1 (de) | 2007-03-12 | 2008-09-17 | Bayer CropScience Aktiengesellschaft | Fluoalkylphenylamidine und deren Verwendung als Fungizide |
US8080688B2 (en) | 2007-03-12 | 2011-12-20 | Bayer Cropscience Ag | 3, 4-disubstituted phenoxyphenylamidines and use thereof as fungicides |
EP1969934A1 (de) | 2007-03-12 | 2008-09-17 | Bayer CropScience AG | 4-Cycloalkyl-oder 4-arylsubstituierte Phenoxyphenylamidine und deren Verwendung als Fungizide |
JP2010520899A (ja) | 2007-03-12 | 2010-06-17 | バイエル・クロツプサイエンス・アクチエンゲゼルシヤフト | ジハロフェノキシフェニルアミジン及び殺真菌剤としてのその使用 |
EP1969930A1 (de) | 2007-03-12 | 2008-09-17 | Bayer CropScience AG | Phenoxyphenylamidine und deren Verwendung als Fungizide |
EP1969929A1 (de) | 2007-03-12 | 2008-09-17 | Bayer CropScience AG | Substituierte Phenylamidine und deren Verwendung als Fungizide |
US8003398B2 (en) | 2007-03-27 | 2011-08-23 | E.I. De Pont De Nemours And Company | Methods and compositions for detecting glyphosate and metabolites thereof |
US7723579B2 (en) | 2007-04-12 | 2010-05-25 | Dow Agrosciences Llc | Canola cultivar DN040244 |
US7723577B2 (en) | 2007-04-12 | 2010-05-25 | Dow Agrosciences Llc | Canola cultivar DN040847 |
AR066045A1 (es) | 2007-04-12 | 2009-07-22 | Dow Agrosciences Llc | Cultivares de canola que tienen alto rendimiento y perfiles estabilizados de acidos grasos |
US7723578B2 (en) | 2007-04-12 | 2010-05-25 | Dow Agrosciences Llc | Canola cultivar DN040839 |
US7723581B2 (en) | 2007-04-12 | 2010-05-25 | Dow Agrosciences Llc | Canola cultivar DN040845 |
US7718852B2 (en) | 2007-04-12 | 2010-05-18 | Dow Agrosciences Llc | Canola cultivar DN040241 |
US7723580B2 (en) | 2007-04-12 | 2010-05-25 | Dow Agrosciences Llc | Canola cultivar DN040844 |
US7723582B2 (en) | 2007-04-12 | 2010-05-25 | Dow Agrosciences Llc | Canola cultivar DN041100 |
US7728195B2 (en) | 2007-04-12 | 2010-06-01 | Dow Agrosciences Llc | Canola cultivar DN040856 |
CA2684340A1 (en) | 2007-04-19 | 2008-10-30 | Bayer Cropscience Ag | Thiadiazolyloxyphenylamidines and the use thereof as fungicides |
DE102007045955A1 (de) | 2007-09-26 | 2009-04-09 | Bayer Cropscience Ag | Wirkstoffkombinationen mit insektiziden und akariziden Eigenschaften |
DE102007045920B4 (de) | 2007-09-26 | 2018-07-05 | Bayer Intellectual Property Gmbh | Synergistische Wirkstoffkombinationen |
DE102007045956A1 (de) | 2007-09-26 | 2009-04-09 | Bayer Cropscience Ag | Wirkstoffkombination mit insektiziden und akariziden Eigenschaften |
DE102007045953B4 (de) | 2007-09-26 | 2018-07-05 | Bayer Intellectual Property Gmbh | Wirkstoffkombinationen mit insektiziden und akariziden Eigenschaften |
DE102007045919B4 (de) | 2007-09-26 | 2018-07-05 | Bayer Intellectual Property Gmbh | Wirkstoffkombinationen mit insektiziden und akariziden Eigenschaften |
DE102007045957A1 (de) | 2007-09-26 | 2009-04-09 | Bayer Cropscience Ag | Wirkstoffkombinationen mit insektiziden und akarziden Eigenschaften |
DE102007045922A1 (de) | 2007-09-26 | 2009-04-02 | Bayer Cropscience Ag | Wirkstoffkombinationen mit insektiziden und akariziden Eigenschaften |
EP2090168A1 (de) | 2008-02-12 | 2009-08-19 | Bayer CropScience AG | Methode zur Verbesserung des Pflanzenwachstums |
AU2008308486B2 (en) | 2007-10-05 | 2014-08-07 | Corteva Agriscience Llc | Methods for transferring molecular substances into plant cells |
EP2072506A1 (de) | 2007-12-21 | 2009-06-24 | Bayer CropScience AG | Thiazolyloxyphenylamidine oder Thiadiazolyloxyphenylamidine und deren Verwendung als Fungizide |
EP2168434A1 (de) | 2008-08-02 | 2010-03-31 | Bayer CropScience AG | Verwendung von Azolen zur Steigerung der Resistenz von Pflanzen oder Pflanzenteilen gegenüber abiotischem Stress |
MX2011001427A (es) | 2008-08-08 | 2011-06-20 | Bayer Bioscience Nv | Metodos para la caracterizacion e identificacion de fibras vegetales. |
WO2010017902A1 (de) | 2008-08-14 | 2010-02-18 | Bayer Cropscience Aktiengesellschaft | Insektizide 4-phenyl-1h-pyrazole |
DE102008041695A1 (de) | 2008-08-29 | 2010-03-04 | Bayer Cropscience Ag | Methoden zur Verbesserung des Pflanzenwachstums |
JP2012507286A (ja) | 2008-11-04 | 2012-03-29 | ダウ アグロサイエンシィズ エルエルシー | オメガ9品質カラシナ |
EP2201838A1 (de) | 2008-12-05 | 2010-06-30 | Bayer CropScience AG | Wirkstoff-Nützlings-Kombinationen mit insektiziden und akariziden Eigenschaften |
EP2198709A1 (de) | 2008-12-19 | 2010-06-23 | Bayer CropScience AG | Verfahren zur Bekämpfung resistenter tierischer Schädlinge |
EP2204094A1 (en) | 2008-12-29 | 2010-07-07 | Bayer CropScience AG | Method for improved utilization of the production potential of transgenic plants Introduction |
EP2223602A1 (de) | 2009-02-23 | 2010-09-01 | Bayer CropScience AG | Verfahren zur verbesserten Nutzung des Produktionspotentials genetisch modifizierter Pflanzen |
US9763451B2 (en) | 2008-12-29 | 2017-09-19 | Bayer Intellectual Property Gmbh | Method for improved use of the production potential of genetically modified plants |
EP2039771A2 (en) | 2009-01-06 | 2009-03-25 | Bayer CropScience AG | Method for improved utilization of the production potential of transgenic plants |
EP2039772A2 (en) | 2009-01-06 | 2009-03-25 | Bayer CropScience AG | Method for improved utilization of the production potential of transgenic plants introduction |
EP2039770A2 (en) | 2009-01-06 | 2009-03-25 | Bayer CropScience AG | Method for improved utilization of the production potential of transgenic plants |
BRPI1006916A8 (pt) | 2009-01-19 | 2016-05-03 | Bayer Cropscience Ag | Dionas cíclicas e seu uso como inseticidas, acaricidas e/ou fungicidas |
EP2227951A1 (de) | 2009-01-23 | 2010-09-15 | Bayer CropScience AG | Verwendung von Enaminocarbonylverbindungen zur Bekämpfung von durch Insekten übertragenen Viren |
JP5592398B2 (ja) | 2009-01-28 | 2014-09-17 | バイエル・クロップサイエンス・アーゲー | 殺菌剤n−シクロアルキル−n−二環式メチレン−カルボキサミド誘導体 |
AR075126A1 (es) | 2009-01-29 | 2011-03-09 | Bayer Cropscience Ag | Metodo para el mejor uso del potencial de produccion de plantas transgenicas |
WO2010093970A2 (en) | 2009-02-13 | 2010-08-19 | Monsanto Technology Llc | Encapsulation of herbicides to reduce crop injury |
EP2398770B1 (en) | 2009-02-17 | 2016-12-28 | Bayer Intellectual Property GmbH | Fungicidal n-(phenylcycloalkyl)carboxamide, n-(benzylcycloalkyl)carboxamide and thiocarboxamide derivatives |
EP2218717A1 (en) | 2009-02-17 | 2010-08-18 | Bayer CropScience AG | Fungicidal N-((HET)Arylethyl)thiocarboxamide derivatives |
TW201031331A (en) | 2009-02-19 | 2010-09-01 | Bayer Cropscience Ag | Pesticide composition comprising a tetrazolyloxime derivative and a fungicide or an insecticide active substance |
DE102009001469A1 (de) | 2009-03-11 | 2009-09-24 | Bayer Cropscience Ag | Verfahren zur verbesserten Nutzung des Produktionspotentials transgener Pflanzen |
DE102009001681A1 (de) | 2009-03-20 | 2010-09-23 | Bayer Cropscience Ag | Verfahren zur verbesserten Nutzung des Produktionspotentials transgener Pflanzen |
DE102009001730A1 (de) | 2009-03-23 | 2010-09-30 | Bayer Cropscience Ag | Verfahren zur verbesserten Nutzung des Produktionspotentials transgener Pflanzen |
DE102009001728A1 (de) | 2009-03-23 | 2010-09-30 | Bayer Cropscience Ag | Verfahren zur verbesserten Nutzung des Produktionspotentials transgener Pflanzen |
DE102009001732A1 (de) | 2009-03-23 | 2010-09-30 | Bayer Cropscience Ag | Verfahren zur verbesserten Nutzung des Produktionspotentials transgener Pflanzen |
WO2010108507A2 (de) | 2009-03-25 | 2010-09-30 | Bayer Cropscience Ag | Synergistische wirkstoffkombinationen |
WO2010108506A1 (de) | 2009-03-25 | 2010-09-30 | Bayer Cropscience Ag | Wirkstoffkombinationen mit insektiziden und akariziden eigenschaften |
EP2232995A1 (de) | 2009-03-25 | 2010-09-29 | Bayer CropScience AG | Verfahren zur verbesserten Nutzung des Produktionspotentials transgener Pflanzen |
CN102395271A (zh) | 2009-03-25 | 2012-03-28 | 拜尔农作物科学股份公司 | 具有杀虫和杀螨特性的活性化合物结合物 |
MX2011009918A (es) | 2009-03-25 | 2011-10-06 | Bayer Cropscience Ag | Combinaciones de principios activos propiedades insecticidas y acaricidas. |
AP3073A (en) | 2009-03-25 | 2014-12-31 | Bayer Cropscience Ag | Active ingredient combinations with insecticidal and acaricidal properties |
EP2239331A1 (en) | 2009-04-07 | 2010-10-13 | Bayer CropScience AG | Method for improved utilization of the production potential of transgenic plants |
CN102458125B (zh) | 2009-05-06 | 2015-04-29 | 拜尔农作物科学股份公司 | 环戊二酮化合物及其用作杀昆虫剂、杀螨剂和/或杀菌剂的用途 |
AR076839A1 (es) | 2009-05-15 | 2011-07-13 | Bayer Cropscience Ag | Derivados fungicidas de pirazol carboxamidas |
EP2251331A1 (en) | 2009-05-15 | 2010-11-17 | Bayer CropScience AG | Fungicide pyrazole carboxamides derivatives |
EP2255626A1 (de) | 2009-05-27 | 2010-12-01 | Bayer CropScience AG | Verwendung von Succinat Dehydrogenase Inhibitoren zur Steigerung der Resistenz von Pflanzen oder Pflanzenteilen gegenüber abiotischem Stress |
UA106618C2 (uk) | 2009-06-02 | 2014-09-25 | Баєр Кропсаєнс Аг | Застосування інгібіторів сукцинатдегідрогенази для контролю підвиду sclerotinia |
EP2451947A1 (en) | 2009-07-07 | 2012-05-16 | Linda A. Castle | Crystal structure of glyphosate acetyltransferase (glyat) and methods of use |
KR20120051015A (ko) | 2009-07-16 | 2012-05-21 | 바이엘 크롭사이언스 아게 | 페닐 트리아졸을 함유하는 상승적 활성 물질 배합물 |
WO2011015524A2 (en) | 2009-08-03 | 2011-02-10 | Bayer Cropscience Ag | Fungicide heterocycles derivatives |
EP2292094A1 (en) | 2009-09-02 | 2011-03-09 | Bayer CropScience AG | Active compound combinations |
NZ598419A (en) | 2009-10-16 | 2013-11-29 | Dow Agrosciences Llc | Use of dendrimer nanotechnology for delivery of biomolecules into plant cells |
US8581046B2 (en) | 2010-11-24 | 2013-11-12 | Pioneer Hi-Bred International, Inc. | Brassica gat event DP-073496-4 and compositions and methods for the identification and/or detection thereof |
EP2343280A1 (en) | 2009-12-10 | 2011-07-13 | Bayer CropScience AG | Fungicide quinoline derivatives |
UY33139A (es) | 2009-12-23 | 2011-07-29 | Bayer Cropscience Ag | Plantas tolerantes a herbicidas inhibidores de las hppd |
BR112012015125A2 (pt) | 2009-12-23 | 2015-09-01 | Bayer Ip Gmbh | "plantas tolerantes aos herbicidas inibidores de hppd" |
AU2010334818B2 (en) | 2009-12-23 | 2015-07-09 | Bayer Intellectual Property Gmbh | Plants tolerant to HPPD inhibitor herbicides |
EP2516635B1 (en) | 2009-12-23 | 2017-11-15 | Bayer Intellectual Property GmbH | Plants tolerant to hppd inhibitor herbicides |
AR079883A1 (es) | 2009-12-23 | 2012-02-29 | Bayer Cropscience Ag | Plantas tolerantes a herbicidas inhibidores de las hppd |
US8796463B2 (en) | 2009-12-28 | 2014-08-05 | Bayer Cropscience Ag | Fungicide hydroximoyl-tetrazole derivatives |
EP2519516A2 (en) | 2009-12-28 | 2012-11-07 | Bayer CropScience AG | Fungicidal hydroximoyl-tetrazole derivatives |
MX336392B (es) | 2009-12-28 | 2016-01-18 | Bayer Cropscience Ag | Derivados de hidroximoil-heterociclos fungicidas. |
PE20121693A1 (es) | 2010-01-22 | 2012-12-01 | Bayer Ip Gmbh | Combinacion de espiromesifeno y abamectina como insecticidas |
US8378177B2 (en) | 2010-02-03 | 2013-02-19 | Dow Agrosciences, Llc | Canola cultivar DN051493 |
AR080443A1 (es) | 2010-03-04 | 2012-04-11 | Bayer Cropscience Ag | 2-amidobencimidazoles sustituidos con fluruoalquilo |
EP2547204A2 (de) | 2010-03-18 | 2013-01-23 | Bayer Intellectual Property GmbH | Aryl- und hetarylsulfonamide als wirkstoffe gegen abiotischen pflanzenstress |
KR20130080005A (ko) | 2010-03-31 | 2013-07-11 | 다우 아그로사이언시즈 엘엘씨 | 식물 세포 내로의 생체분자의 전달을 위한 식물 펩티드 감마-제인 |
BR112012025714A2 (pt) | 2010-04-06 | 2015-09-08 | Bayer Ip Gmbh | uso de ácido 4-fenilbutírico e/ou sais do mesmo para aumentar a tolerância a estresse de plantas |
JP6046604B2 (ja) | 2010-04-09 | 2016-12-21 | バイエル・インテレクチュアル・プロパティ・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツングBayer Intellectual Property GmbH | 非生物的ストレスに対する植物の耐性を増強させるための(1−シアノシクロプロピル)フェニルホスフィン酸の誘導体、それらのエステル及び/又はそれらの塩の使用 |
WO2011134911A2 (en) | 2010-04-28 | 2011-11-03 | Bayer Cropscience Ag | Fungicide hydroximoyl-tetrazole derivatives |
US20130116287A1 (en) | 2010-04-28 | 2013-05-09 | Christian Beier | Fungicide hydroximoyl-heterocycles derivatives |
JP2013525400A (ja) | 2010-04-28 | 2013-06-20 | バイエル・クロップサイエンス・アーゲー | 殺菌剤ヒドロキシモイル−複素環誘導体 |
ES2533026T3 (es) | 2010-06-03 | 2015-04-07 | Bayer Intellectual Property Gmbh | N-[(het)arilalquil)]pirazol (tio)carboxamidas y sus análogos heterosustituidos |
UA110703C2 (uk) | 2010-06-03 | 2016-02-10 | Байєр Кропсайнс Аг | Фунгіцидні похідні n-[(тризаміщений силіл)метил]-карбоксаміду |
BR112012030607B1 (pt) | 2010-06-03 | 2018-06-26 | Bayer Cropscience Ag | Composto, composição fungicida e método para controlar fungos fitopatogênicos de culturas |
WO2011154159A1 (en) | 2010-06-09 | 2011-12-15 | Bayer Bioscience N.V. | Methods and means to modify a plant genome at a nucleotide sequence commonly used in plant genome engineering |
AU2011264074B2 (en) | 2010-06-09 | 2015-01-22 | Bayer Cropscience Nv | Methods and means to modify a plant genome at a nucleotide sequence commonly used in plant genome engineering |
US8653327B2 (en) | 2010-07-07 | 2014-02-18 | Agrigenetics, Inc. | Linear DNA molecule delivery using PEGylated quantum dots for stable transformation in plants |
US9173399B2 (en) | 2010-07-20 | 2015-11-03 | Bayer Intellectual Property Gmbh | Benzocycloalkenes as antifungal agents |
AU2011289286B2 (en) | 2010-08-13 | 2015-02-12 | Pioneer Hi-Bred International, Inc. | Methods and compositions for targeting sequences of interest to the chloroplast |
EP2605646B1 (en) | 2010-08-18 | 2016-07-20 | Monsanto Technology LLC | Early applications of encapsulated acetamides for reduced injury in crops |
RU2013114710A (ru) | 2010-09-03 | 2014-10-10 | Байер Интеллектуэль Проперти Гмбх | Замещенные конденсированные пиримидиноны и дигидропиримидиноны |
EP2460406A1 (en) | 2010-12-01 | 2012-06-06 | Bayer CropScience AG | Use of fluopyram for controlling nematodes in nematode resistant crops |
US20140056866A1 (en) | 2010-09-22 | 2014-02-27 | Bayer Intellectual Property Gmbh | Use of biological or chemical control agents for controlling insects and nematodes in resistant crops |
KR101871525B1 (ko) | 2010-10-07 | 2018-06-26 | 바이엘 크롭사이언스 악티엔게젤샤프트 | 테트라졸릴옥심 유도체 및 티아졸릴피페리딘 유도체를 포함하는 살진균제 조성물 |
MX2013004278A (es) | 2010-10-21 | 2013-06-05 | Bayer Ip Gmbh | N-bencil carboxamidas heterociclicas. |
CN103313977B (zh) | 2010-10-21 | 2015-06-03 | 拜耳知识产权有限责任公司 | 1-(杂环羰基)哌啶 |
KR20130116074A (ko) | 2010-11-02 | 2013-10-22 | 바이엘 인텔렉쳐 프로퍼티 게엠베하 | N-헤타릴메틸 피라졸릴카르복사미드 |
EP2669370B1 (en) | 2010-11-10 | 2016-06-01 | Bayer CropScience AG | HPPD variants and methods of use |
AR083874A1 (es) | 2010-11-15 | 2013-03-27 | Bayer Cropscience Ag | 5-halogenopirazol(tio)carboxamidas |
US9375004B2 (en) | 2010-11-15 | 2016-06-28 | Bayer Intellectual Property Gmbh | 5-halogenopyrazolecarboxamides |
WO2012065944A1 (en) | 2010-11-15 | 2012-05-24 | Bayer Cropscience Ag | N-aryl pyrazole(thio)carboxamides |
US8575431B2 (en) | 2010-11-24 | 2013-11-05 | Pioneer Hi-Bred International, Inc. | Brassica GAT event DP-061061-7 and compositions and methods for the identification and/or detection thereof |
WO2012072660A1 (en) | 2010-12-01 | 2012-06-07 | Bayer Cropscience Ag | Use of fluopyram for controlling nematodes in crops and for increasing yield |
EP2460407A1 (de) | 2010-12-01 | 2012-06-06 | Bayer CropScience AG | Wirkstoffkombinationen umfassend Pyridylethylbenzamide und weitere Wirkstoffe |
JP2014502611A (ja) | 2010-12-29 | 2014-02-03 | バイエル・インテレクチユアル・プロパテイー・ゲー・エム・ベー・ハー | 殺菌剤ヒドロキシモイル−テトラゾール誘導体 |
EP2474542A1 (en) | 2010-12-29 | 2012-07-11 | Bayer CropScience AG | Fungicide hydroximoyl-tetrazole derivatives |
US8563811B2 (en) | 2010-12-30 | 2013-10-22 | Agrigenetics, Inc. | Canola cultivar DN040845A |
US8563810B2 (en) | 2010-12-30 | 2013-10-22 | Agrigenetics, Inc. | Canola cultivar DN040244A |
US8530726B2 (en) | 2010-12-30 | 2013-09-10 | Agrigenetics, Inc. | Canola cultivar G030994 |
US8558064B2 (en) | 2010-12-30 | 2013-10-15 | Agrigenetics, Inc. | Canola cultivar CL31613 |
EP2471363A1 (de) | 2010-12-30 | 2012-07-04 | Bayer CropScience AG | Verwendung von Aryl-, Heteroaryl- und Benzylsulfonamidocarbonsäuren, -carbonsäureestern, -carbonsäureamiden und -carbonitrilen oder deren Salze zur Steigerung der Stresstoleranz in Pflanzen |
US9603322B2 (en) | 2010-12-30 | 2017-03-28 | Agrigenetics, Inc. | Canola cultivars having high yield and stabilized fatty acid profiles |
US8558065B2 (en) | 2010-12-30 | 2013-10-15 | Agrigenetics, Inc. | Canola cultivar G31064 |
CA2827901C (en) | 2011-02-22 | 2018-11-13 | Agrigenetics, Inc. | Canola germplasm exhibiting seed compositional attributes that deliver enhanced canola meal nutritional value having omega-9 traits |
EP2494867A1 (de) | 2011-03-01 | 2012-09-05 | Bayer CropScience AG | Halogen-substituierte Verbindungen in Kombination mit Fungiziden |
CA2823999C (en) | 2011-03-10 | 2020-03-24 | Bayer Intellectual Property Gmbh | Use of lipochito-oligosaccharide compounds for safeguarding seed safety of treated seeds |
EP2686311A1 (en) | 2011-03-14 | 2014-01-22 | Bayer Intellectual Property GmbH | Fungicide hydroximoyl-tetrazole derivatives |
CN103562397A (zh) | 2011-03-23 | 2014-02-05 | 陶氏益农公司 | 适合于在植物中成像和投递应用的量子点载体肽缀合物 |
AU2012234448A1 (en) | 2011-03-25 | 2013-10-17 | Bayer Intellectual Property Gmbh | Use of N-(1,2,5-Oxadiazol-3-yl)benzamides for controlling unwanted plants in areas of transgenic crop plants being tolerant to HPPD inhibitor herbicides |
PL2688406T3 (pl) | 2011-03-25 | 2015-10-30 | Bayer Ip Gmbh | Zastosowanie N-(tetrazol-4-ilo)- lub N-(triazol-3-ilo)arylokarboksamidów lub ich soli do zwalczania niepożądanych roślin w obszarach transgenicznych roślin uprawnych tolerancyjnych na środki chwastobójcze będące inhibitorem HPPD |
EP2694494A1 (en) | 2011-04-08 | 2014-02-12 | Bayer Intellectual Property GmbH | Fungicide hydroximoyl-tetrazole derivatives |
EP2511255A1 (de) | 2011-04-15 | 2012-10-17 | Bayer CropScience AG | Substituierte Prop-2-in-1-ol- und Prop-2-en-1-ol-Derivate |
AR085568A1 (es) | 2011-04-15 | 2013-10-09 | Bayer Cropscience Ag | 5-(biciclo[4.1.0]hept-3-en-2-il)-penta-2,4-dienos y 5-(biciclo[4.1.0]hept-3-en-2-il)-pent-2-en-4-inos sustituidos como principios activos contra el estres abiotico de las plantas |
AR090010A1 (es) | 2011-04-15 | 2014-10-15 | Bayer Cropscience Ag | 5-(ciclohex-2-en-1-il)-penta-2,4-dienos y 5-(ciclohex-2-en-1-il)-pent-2-en-4-inos sustituidos como principios activos contra el estres abiotico de las plantas, usos y metodos de tratamiento |
AR085585A1 (es) | 2011-04-15 | 2013-10-09 | Bayer Cropscience Ag | Vinil- y alquinilciclohexanoles sustituidos como principios activos contra estres abiotico de plantas |
TR201901837T4 (tr) | 2011-04-22 | 2019-03-21 | Bayer Cropscience Ag | Bir (tiyo)karboksamit türevi ve bir mantar öldürücü bileşik içeren etkin bileşik terkipleri. |
EP2718443B1 (en) | 2011-06-06 | 2017-11-29 | Bayer CropScience NV | Methods and means to modify a plant genome at a preselected site |
JP2014520776A (ja) | 2011-07-04 | 2014-08-25 | バイエル・インテレクチユアル・プロパテイー・ゲー・エム・ベー・ハー | 植物における非生物的ストレスに対する活性薬剤としての置換されているイソキノリノン類、イソキノリンジオン類、イソキノリントリオン類およびジヒドロイソキノリノン類または各場合でのそれらの塩の使用 |
IN2014DN00156A (zh) | 2011-08-10 | 2015-05-22 | Bayer Ip Gmbh | |
EP2742059A1 (en) | 2011-08-12 | 2014-06-18 | Bayer CropScience NV | Guard cell-specific expression of transgenes in cotton |
CN103890181A (zh) | 2011-08-22 | 2014-06-25 | 拜尔作物科学公司 | 修饰植物基因组的方法和手段 |
CN103748092A (zh) | 2011-08-22 | 2014-04-23 | 拜耳知识产权有限责任公司 | 杀真菌剂肟基-四唑衍生物 |
EP2561759A1 (en) | 2011-08-26 | 2013-02-27 | Bayer Cropscience AG | Fluoroalkyl-substituted 2-amidobenzimidazoles and their effect on plant growth |
US20140221210A1 (en) | 2011-09-09 | 2014-08-07 | Peter Dahmen | Acyl-homoserine lactone derivatives for improving plant yield |
EP2755949B1 (en) | 2011-09-12 | 2015-10-21 | Bayer Intellectual Property GmbH | Fungicidal 4-substituted-3-{phenyl[(heterocyclylmethoxy)imino]methyl}-1,2,4-oxadizol-5(4h)-one derivatives |
CN108552186A (zh) | 2011-09-16 | 2018-09-21 | 拜耳知识产权有限责任公司 | 苯基吡唑啉-3-甲酸酯类用于提高植物产量的用途 |
WO2013037956A1 (en) | 2011-09-16 | 2013-03-21 | Bayer Intellectual Property Gmbh | Use of 5-phenyl- or 5-benzyl-2 isoxazoline-3 carboxylates for improving plant yield |
UA115971C2 (uk) | 2011-09-16 | 2018-01-25 | Байєр Інтеллектуал Проперті Гмбх | Застосування ацилсульфонамідів для покращення врожайності рослин |
CN103929964A (zh) | 2011-09-23 | 2014-07-16 | 拜耳知识产权有限责任公司 | 4-取代的1-苯基吡唑-3-甲酸衍生物作为对抗非生物植物胁迫的活性物质的用途 |
JP6255344B2 (ja) | 2011-10-04 | 2017-12-27 | バイエル・インテレクチュアル・プロパティ・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツングBayer Intellectual Property GmbH | サッカロピンデヒドロゲナーゼ遺伝子を阻害することによって真菌類及び卵菌類を防除するためのRNAi |
WO2013050324A1 (de) | 2011-10-06 | 2013-04-11 | Bayer Intellectual Property Gmbh | Abiotischen pflanzenstress-reduzierende kombination enthaltend 4- phenylbuttersäure (4-pba) oder eines ihrer salze (komponente (a)) und eine oder mehrere ausgewählte weitere agronomisch wirksame verbindungen (komponente(n) (b) |
US9204601B1 (en) | 2011-11-21 | 2015-12-08 | Agrigenetics, Inc. | Canola inbred CL60855R |
US9210857B1 (en) | 2011-11-21 | 2015-12-15 | Agrigenetics, Inc. | Canola inbred CL102407R |
US9204602B1 (en) | 2011-11-21 | 2015-12-08 | Agrigenetics, Inc. | Canola inbred CL77606R |
MX2014005976A (es) | 2011-11-21 | 2014-08-27 | Bayer Ip Gmbh | Derivados de n-[(silil trisustituido)metil]-carboxamida fungicidas. |
RU2014126063A (ru) | 2011-11-30 | 2016-01-27 | Байер Интеллекчуал Проперти Гмбх | ФУНГИЦИДНЫЕ N-БИЦИКЛОАЛКИЛ и N-ТРИЦИКЛОАЛКИЛ(ТИО)КАРБОКСАМИДНЫЕ ПРОИЗВОДНЫЕ |
WO2013092519A1 (en) | 2011-12-19 | 2013-06-27 | Bayer Cropscience Ag | Use of anthranilic acid diamide derivatives for pest control in transgenic crops |
CN104470896B (zh) | 2011-12-29 | 2016-11-09 | 拜耳知识产权有限责任公司 | 杀真菌的3-[(吡啶-2-基甲氧基亚氨基)(苯基)甲基]-2-取代的-1,2,4-噁二唑-5(2h)-酮衍生物 |
TWI558701B (zh) | 2011-12-29 | 2016-11-21 | 拜耳知識產權公司 | 殺真菌之3-[(1,3-噻唑-4-基甲氧基亞胺)(苯基)甲基]-2-經取代之-1,2,4-二唑-5(2h)-酮衍生物 |
NZ722687A (en) | 2012-02-22 | 2017-03-31 | Bayer Ip Gmbh | Use of succinate dehydrogenase inhibitors (sdhis) for controlling wood diseases in grape. |
CA2865300C (en) | 2012-02-27 | 2021-02-16 | Bayer Intellectual Property Gmbh | Active compound combinations containing a thiazoylisoxazoline and a fungicide |
WO2013139949A1 (en) | 2012-03-23 | 2013-09-26 | Bayer Intellectual Property Gmbh | Compositions comprising a strigolactame compound for enhanced plant growth and yield |
US9357778B2 (en) | 2012-04-12 | 2016-06-07 | Bayer Cropscience Ag | N-acyl-2-(cyclo)alkypyrrolidines and piperidines useful as fungicides |
CA2865599C (en) | 2012-04-20 | 2020-10-27 | Bayer Cropscience Ag | N-cycloalkyl-n-[(heterocyclylphenyl)methylene]-(thio)carboxamide derivatives |
WO2013156560A1 (en) | 2012-04-20 | 2013-10-24 | Bayer Cropscience Ag | N-cycloalkyl-n-[(trisubstitutedsilylphenyl)methylene]-(thio)carboxamide derivatives |
US11518997B2 (en) | 2012-04-23 | 2022-12-06 | BASF Agricultural Solutions Seed US LLC | Targeted genome engineering in plants |
BR112014027644A2 (pt) | 2012-05-09 | 2017-06-27 | Bayer Cropscience Ag | 5-halogenopirazol-indanil-carboxamidas |
EP2662362A1 (en) | 2012-05-09 | 2013-11-13 | Bayer CropScience AG | Pyrazole indanyl carboxamides |
EP2662360A1 (en) | 2012-05-09 | 2013-11-13 | Bayer CropScience AG | 5-Halogenopyrazole indanyl carboxamides |
EP2662361A1 (en) | 2012-05-09 | 2013-11-13 | Bayer CropScience AG | Pyrazol indanyl carboxamides |
EP2662363A1 (en) | 2012-05-09 | 2013-11-13 | Bayer CropScience AG | 5-Halogenopyrazole biphenylcarboxamides |
JP6262208B2 (ja) | 2012-05-09 | 2018-01-17 | バイエル・クロップサイエンス・アクチェンゲゼルシャフト | ピラゾールインダニルカルボキサミド類 |
EP2662364A1 (en) | 2012-05-09 | 2013-11-13 | Bayer CropScience AG | Pyrazole tetrahydronaphthyl carboxamides |
EP2662370A1 (en) | 2012-05-09 | 2013-11-13 | Bayer CropScience AG | 5-Halogenopyrazole benzofuranyl carboxamides |
AR091104A1 (es) | 2012-05-22 | 2015-01-14 | Bayer Cropscience Ag | Combinaciones de compuestos activos que comprenden un derivado lipo-quitooligosacarido y un compuesto nematicida, insecticida o fungicida |
CN104540951B (zh) | 2012-06-07 | 2017-07-04 | 美国陶氏益农公司 | 使用芸苔属双向组成型启动子表达转基因的构建体和方法 |
AU2013289301A1 (en) | 2012-07-11 | 2015-01-22 | Bayer Cropscience Ag | Use of fungicidal combinations for increasing the tolerance of a plant towards abiotic stress |
EP2877581B1 (en) | 2012-07-26 | 2017-11-29 | Dow AgroSciences LLC | High-throughput dna fragment assembly |
US9688996B2 (en) | 2012-08-17 | 2017-06-27 | Dow Agrosciences Llc | Use of a maize untranslated region for transgene expression in plants |
EP2892345A1 (de) | 2012-09-05 | 2015-07-15 | Bayer CropScience AG | Verwendung substituierter 2-amidobenzimidazole, 2-amidobenzoxazole und 2-amidobenzothiazole oder deren salze als wirkstoffe gegen abiotischen pflanzenstress |
UA118090C2 (uk) | 2012-09-07 | 2018-11-26 | ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі | Спосіб інтегрування послідовності нуклеїнової кислоти, що представляє інтерес, у ген fad2 у клітині сої та специфічний для локусу fad2 білок, що зв'язується, здатний індукувати спрямований розрив |
AU2013312538B2 (en) | 2012-09-07 | 2019-01-24 | Corteva Agriscience Llc | FAD3 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks |
KR20150070148A (ko) | 2012-09-14 | 2015-06-24 | 바이엘 크롭사이언스 엘피 | Hppd 변이체들 및 사용 방법들 |
WO2014060518A1 (en) | 2012-10-19 | 2014-04-24 | Bayer Cropscience Ag | Method of plant growth promotion using carboxamide derivatives |
CN105451556B (zh) | 2012-10-19 | 2017-11-14 | 拜尔农科股份公司 | 使用羧酰胺或硫代羧酰胺衍生物处理植物以抵抗对杀真菌剂具有抗性的真菌的方法 |
US20150250176A1 (en) | 2012-10-19 | 2015-09-10 | Bayer Cropscience Ag | Method for enhancing tolerance to abiotic stress in plants using carboxamide or thiocarboxamide derivatives |
JP6153619B2 (ja) | 2012-10-19 | 2017-06-28 | バイエル・クロップサイエンス・アクチェンゲゼルシャフト | カルボキサミド誘導体を含む活性化合物の組み合わせ |
WO2014079957A1 (de) | 2012-11-23 | 2014-05-30 | Bayer Cropscience Ag | Selektive inhibition der ethylensignaltransduktion |
EP2735231A1 (en) | 2012-11-23 | 2014-05-28 | Bayer CropScience AG | Active compound combinations |
US9447430B1 (en) | 2012-11-29 | 2016-09-20 | Agrigenetics, Inc. | Canola inbred line G2X0023AB |
US9414556B1 (en) | 2012-11-29 | 2016-08-16 | Agrigenetics, Inc. | Canola inbred restorer line G98014R |
US9445564B1 (en) | 2012-11-29 | 2016-09-20 | Agrigenetics, Inc. | Canola inbred line DN051465A |
CA2892712A1 (en) | 2012-11-30 | 2014-06-05 | Bayer Cropscience Ag | Ternary fungicidal and pesticidal mixtures |
UA116223C2 (uk) | 2012-11-30 | 2018-02-26 | Байєр Кропсайєнс Акцієнгезелльшафт | Подвійна фунгіцидна суміш |
US9775349B2 (en) | 2012-11-30 | 2017-10-03 | Bayer Cropscience Ag | Binary fungicidal or pesticidal mixture |
CA2892701A1 (en) | 2012-11-30 | 2014-06-05 | Bayer Cropscience Ag | Binary pesticidal and fungicidal mixtures |
BR112015012055B1 (pt) | 2012-11-30 | 2021-01-12 | Bayer Cropscience Ag | composição fungicida ternária, seu processo de preparação, método para controlar um ou mais microrganismos nocivos, semente resistente a microrganismos nocivos e seu método de tratamento |
EP2740720A1 (de) | 2012-12-05 | 2014-06-11 | Bayer CropScience AG | Substituierte bicyclische- und tricyclische Pent-2-en-4-insäure -Derivate und ihre Verwendung zur Steigerung der Stresstoleranz in Pflanzen |
BR112015012926A2 (pt) | 2012-12-05 | 2017-07-11 | Bayer Cropscience Ag | uso de 1-(aril etinil)-, 1-(heteroaril etinil)-, 1-(heterociclil etinil)- substituído e 1-(cicloalquenil etinil)-ciclohexanóis como agentes ativos contra o estresse abiótico da planta |
EP2740356A1 (de) | 2012-12-05 | 2014-06-11 | Bayer CropScience AG | Substituierte (2Z)-5(1-Hydroxycyclohexyl)pent-2-en-4-insäure-Derivate |
WO2014090765A1 (en) | 2012-12-12 | 2014-06-19 | Bayer Cropscience Ag | Use of 1-[2-fluoro-4-methyl-5-(2,2,2-trifluoroethylsulfinyl)phenyl]-5-amino-3-trifluoromethyl)-1 h-1,2,4 tfia zole for controlling nematodes in nematode-resistant crops |
US20140173781A1 (en) | 2012-12-13 | 2014-06-19 | Pioneer Hi-Bred International, Inc. | Methods and compositions for producing and selecting transgenic wheat plants |
AR093996A1 (es) | 2012-12-18 | 2015-07-01 | Bayer Cropscience Ag | Combinaciones bactericidas y fungicidas binarias |
EP2935218A1 (en) | 2012-12-19 | 2015-10-28 | Bayer CropScience AG | Difluoromethyl-nicotinic- tetrahydronaphtyl carboxamides |
BR112015015055A2 (pt) | 2012-12-21 | 2017-10-03 | Pioneer Hi Bred Int | Método para desintoxicar um herbicida análogo de auxina, método para controlar pelo menos uma erva em uma área de cultivo, método para testar uma resposta de planta a um ou mais compostos |
US20140203176A1 (en) | 2013-01-23 | 2014-07-24 | Dow Agrosciences Llc | Systems and methods for real-time sampling and analysis of biomolecules beneath the surface of biological tissue |
US9426953B1 (en) | 2013-02-21 | 2016-08-30 | Agrigenetics, Inc. | Canola hybrid cultivar CE216910H |
US9538716B1 (en) | 2013-02-21 | 2017-01-10 | Agrigenetics, Inc. | Canola inbred restorer line CE185942R |
US9414557B1 (en) | 2013-02-21 | 2016-08-16 | Agrigenetics, Inc. | Canola inbred restorer line CE185952R |
CN105705490A (zh) | 2013-03-07 | 2016-06-22 | 拜耳作物科学股份公司 | 杀真菌的3-{苯基[(杂环基甲氧基)亚氨基]甲基}-杂环衍生物 |
WO2014153242A1 (en) | 2013-03-14 | 2014-09-25 | Pioneer Hi-Bred International, Inc. | Compositions having dicamba decarboxylase activity and methods of use |
EP2970995A1 (en) | 2013-03-14 | 2016-01-20 | Pioneer Hi-Bred International, Inc. | Compositions having dicamba decarboxylase activity and methods of use |
CN105121650A (zh) | 2013-04-02 | 2015-12-02 | 拜尔作物科学公司 | 真核生物中的靶向基因组工程 |
WO2014167008A1 (en) | 2013-04-12 | 2014-10-16 | Bayer Cropscience Ag | Novel triazolinthione derivatives |
JP6397482B2 (ja) | 2013-04-12 | 2018-09-26 | バイエル・クロップサイエンス・アクチェンゲゼルシャフト | 新規トリアゾール誘導体 |
WO2014170364A1 (en) | 2013-04-19 | 2014-10-23 | Bayer Cropscience Ag | Binary insecticidal or pesticidal mixture |
MX358633B (es) | 2013-04-19 | 2018-08-28 | Bayer Cropscience Ag | Metodo de uso mejorado del potencial de produccion de plantas transgenicas. |
TW201507722A (zh) | 2013-04-30 | 2015-03-01 | Bayer Cropscience Ag | 做為殺線蟲劑及殺體內寄生蟲劑的n-(2-鹵素-2-苯乙基)-羧醯胺類 |
WO2014177514A1 (en) | 2013-04-30 | 2014-11-06 | Bayer Cropscience Ag | Nematicidal n-substituted phenethylcarboxamides |
CN105636939B (zh) | 2013-06-26 | 2018-08-31 | 拜耳作物科学股份公司 | N-环烷基-n-[(二环基苯基)亚甲基]-(硫代)甲酰胺衍生物 |
WO2015004040A1 (de) | 2013-07-09 | 2015-01-15 | Bayer Cropscience Ag | Verwendung ausgewählter pyridoncarboxamide oder deren salzen als wirkstoffe gegen abiotischen pflanzenstress |
EP2837287A1 (en) | 2013-08-15 | 2015-02-18 | Bayer CropScience AG | Use of prothioconazole for increasing root growth of Brassicaceae |
EP3049517B1 (en) | 2013-09-24 | 2018-04-11 | Bayer CropScience NV | Hetero-transglycosylase and uses thereof |
AU2014334590A1 (en) | 2013-10-18 | 2016-04-28 | Pioneer Hi-Bred International, Inc. | Glyphosate-N-acetyltransferase (GLYAT) sequences and methods of use |
UY35814A (es) | 2013-11-04 | 2015-05-29 | Dow Agrosciences Llc | ?lugares óptimos para la soja?. |
TWI671404B (zh) | 2013-11-04 | 2019-09-11 | 美商陶氏農業科學公司 | 最適玉米基因座(二) |
US9909131B2 (en) | 2013-11-04 | 2018-03-06 | Dow Agrosciences Llc | Optimal soybean loci |
UA120502C2 (uk) | 2013-11-04 | 2019-12-26 | Дау Агросайєнсиз Елелсі | Спосіб отримання трансгенної рослини маїсу |
CA2932484A1 (en) | 2013-12-05 | 2015-06-11 | Bayer Cropscience Aktiengesellschaft | N-cycloalkyl-n-{[2-(1-substitutedcycloalkyl)phenyl]methylene}-(thio)carboxamide derivatives |
US10071967B2 (en) | 2013-12-05 | 2018-09-11 | Bayer Cropscience Aktiengesellschaft | N-cycloalkyl-N-{[2-(1-substitutedcycloalkyl)phenyl]methylene}-(thio)carboxamide derivatives |
US10683513B2 (en) | 2013-12-31 | 2020-06-16 | Dow Agrosciences Llc | Tissue-specific expression and hybrid plant production |
US9596816B1 (en) | 2014-02-28 | 2017-03-21 | Agrigenetics, Inc. | Canola inbred restorer line CL215695R |
US9844195B1 (en) | 2014-02-28 | 2017-12-19 | Agrigenetics, Inc. | Canola hybrid cultivar CL2537387H |
US9554534B1 (en) | 2014-02-28 | 2017-01-31 | Agrigenetics, Inc. | Canola inbred line CL1992625A |
US9854763B1 (en) | 2014-02-28 | 2018-01-02 | Agrigenetics, Inc. | Canola inbred line CL1992625B |
CA2942171C (en) | 2014-03-11 | 2023-05-09 | Bayer Cropscience Lp | Hppd variants and methods of use |
US10053702B2 (en) | 2014-04-22 | 2018-08-21 | E I Du Pont De Nemours And Company | Plastidic carbonic anhydrase genes for oil augmentation in seeds with increased DGAT expression |
AR101214A1 (es) | 2014-07-22 | 2016-11-30 | Bayer Cropscience Ag | Ciano-cicloalquilpenta-2,4-dienos, ciano-cicloalquilpent-2-en-4-inas, ciano-heterociclilpenta-2,4-dienos y ciano-heterociclilpent-2-en-4-inas sustituidos como principios activos contra el estrés abiótico de plantas |
US9986702B1 (en) | 2014-12-05 | 2018-06-05 | Agrigenetics, Inc. | Canola inbred restorer line G1934899R |
US10165751B2 (en) | 2014-12-05 | 2019-01-01 | Agrigenetics, Inc. | Canola inbred line G30853A |
AR103024A1 (es) | 2014-12-18 | 2017-04-12 | Bayer Cropscience Ag | Piridoncarboxamidas seleccionadas o sus sales como sustancias activas contra estrés abiótico de las plantas |
US9968047B2 (en) | 2015-03-24 | 2018-05-15 | Agrigenetics, Inc. | Canola hybrid cultivar CL2562968H |
US10314270B2 (en) | 2015-04-01 | 2019-06-11 | Agrigenetics, Inc. | Canola hybrid cultivar G3697124H |
US10214510B2 (en) | 2015-04-13 | 2019-02-26 | Bayer Cropscience Aktiengesellschaft | N-cycloalkyl-N-(biheterocyclylethylene)-(thio)carboxamide derivatives |
US9968050B2 (en) | 2015-04-15 | 2018-05-15 | Agrigenetics, Inc. | Canola inbred restorer line G175274R |
US10306852B2 (en) | 2015-04-15 | 2019-06-04 | Agrigenetics, Inc. | Canola inbred line G1992650A |
US9968051B2 (en) | 2015-04-15 | 2018-05-15 | Agrigenetics, Inc. | Canola hybrid cultivar G2537376H |
US9974262B2 (en) | 2015-04-15 | 2018-05-22 | Agrigenetics, Inc. | Canola inbred restorer line CL134904R |
EP3436575A1 (en) | 2015-06-18 | 2019-02-06 | The Broad Institute Inc. | Novel crispr enzymes and systems |
CN108884470B (zh) | 2015-09-11 | 2023-07-25 | 巴斯夫农业种子解决方案美国有限责任公司 | Hppd变体及使用方法 |
EP3374907B1 (en) | 2015-11-10 | 2022-10-19 | Corteva Agriscience LLC | Methods and systems for predicting the risk of transgene silencing |
BR112019001764A2 (pt) | 2016-07-29 | 2019-05-07 | Bayer Cropscience Ag | combinações de compostos ativos e métodos para proteção de material de propagação de plantas |
US10420296B2 (en) | 2016-09-07 | 2019-09-24 | Agrigenetics, Inc. | Canola inbred restorer line G263532R |
US10588281B2 (en) | 2016-09-07 | 2020-03-17 | Agrigenetics, Inc. | Canola hybrid cultivar G5428584H |
US10426110B2 (en) | 2016-09-07 | 2019-10-01 | Agrigenetics, Inc. | Canola inbred restorer line CL2503899R |
US10244716B2 (en) | 2016-09-07 | 2019-04-02 | Agrigenetics, Inc. | Canola hybrid cultivar CL3701975H |
US10463004B2 (en) | 2016-09-07 | 2019-11-05 | Agrigenetics, Inc. | Canola inbred line G1466454A/B |
CN109715622A (zh) | 2016-09-22 | 2019-05-03 | 拜耳作物科学股份公司 | 新的三唑衍生物及其作为杀真菌剂的用途 |
US20190211002A1 (en) | 2016-09-22 | 2019-07-11 | Bayer Cropscience Aktiengesellschaft | Novel triazole derivatives |
US20190225974A1 (en) | 2016-09-23 | 2019-07-25 | BASF Agricultural Solutions Seed US LLC | Targeted genome optimization in plants |
CN109890204A (zh) | 2016-10-26 | 2019-06-14 | 拜耳作物科学股份公司 | Pyraziflumid用于在种子处理应用中控制核盘菌属种的用途 |
WO2018104392A1 (en) | 2016-12-08 | 2018-06-14 | Bayer Cropscience Aktiengesellschaft | Use of insecticides for controlling wireworms |
EP3332645A1 (de) | 2016-12-12 | 2018-06-13 | Bayer Cropscience AG | Verwendung substituierter pyrimidindione oder jeweils deren salze als wirkstoffe gegen abiotischen pflanzenstress |
WO2018108627A1 (de) | 2016-12-12 | 2018-06-21 | Bayer Cropscience Aktiengesellschaft | Verwendung substituierter indolinylmethylsulfonamide oder deren salze zur steigerung der stresstoleranz in pflanzen |
US11708565B2 (en) | 2017-03-07 | 2023-07-25 | BASF Agricultural Solutions Seesi US LLC | HPPD variants and methods of use |
WO2018204777A2 (en) | 2017-05-05 | 2018-11-08 | The Broad Institute, Inc. | Methods for identification and modification of lncrna associated with target genotypes and phenotypes |
WO2019025153A1 (de) | 2017-07-31 | 2019-02-07 | Bayer Cropscience Aktiengesellschaft | Verwendung von substituierten n-sulfonyl-n'-aryldiaminoalkanen und n-sulfonyl-n'-heteroaryldiaminoalkanen oder deren salzen zur steigerung der stresstoleranz in pflanzen |
CA3073848A1 (en) | 2017-09-21 | 2019-03-28 | The Broad Institute, Inc. | Systems, methods, and compositions for targeted nucleic acid editing |
WO2019083808A1 (en) | 2017-10-24 | 2019-05-02 | Basf Se | IMPROVING HERBICIDE TOLERANCE AGAINST HPPD INHIBITORS BY REGULATION OF PUTATIVE REDUCED 4-HYDROXYPHENYLPYRUVATE REDUCES IN SOYBEANS |
BR112020008092A2 (pt) | 2017-10-24 | 2020-09-15 | BASF Agricultural Solutions Seed US LLC | método para conferir tolerância a um herbicida e planta de soja transgênica |
US10968257B2 (en) | 2018-04-03 | 2021-04-06 | The Broad Institute, Inc. | Target recognition motifs and uses thereof |
EP3802521A1 (de) | 2018-06-04 | 2021-04-14 | Bayer Aktiengesellschaft | Herbizid wirksame bizyklische benzoylpyrazole |
BR112020026640A2 (pt) | 2018-06-28 | 2021-04-06 | Pioneer Hi-Bred International, Inc. | Métodos para selecionar plantas transformadas |
CA3107382A1 (en) | 2018-07-26 | 2020-01-30 | Bayer Aktiengesellschaft | Use of the succinate dehydrogenase inhibitor fluopyram for controlling root rot complex and/or seedling disease complex caused by rhizoctonia solani, fusarium species and pythium species in brassicaceae species |
CN112714614A (zh) | 2018-09-17 | 2021-04-27 | 拜耳公司 | 杀真菌剂异氟普仑用于在谷物中防治麦角菌和减少菌核的用途 |
US20220039383A1 (en) | 2018-09-17 | 2022-02-10 | Bayer Aktiengesellschaft | Use of the Succinate Dehydrogenase Inhibitor Fluopyram for Controlling Claviceps Purpurea and Reducing Sclerotia in Cereals |
US20210395758A1 (en) | 2018-10-31 | 2021-12-23 | Pioneer Hi-Bred International, Inc. | Compositions and methods for ochrobactrum-mediated plant transformation |
WO2020131862A1 (en) | 2018-12-17 | 2020-06-25 | The Broad Institute, Inc. | Crispr-associated transposase systems and methods of use thereof |
EP3917319A4 (en) | 2019-01-30 | 2022-11-23 | Monsanto Technology LLC | MICROENCAPSULATED ACETAMIDE HERBICIDES |
EP3938521A1 (en) | 2019-03-11 | 2022-01-19 | Pioneer Hi-Bred International, Inc. | Methods for clonal plant production |
CA3127173A1 (en) | 2019-03-28 | 2020-10-01 | Pioneer Hi-Bred International, Inc. | Modified agrobacterium strains and use thereof for plant transformation |
Family Cites Families (152)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US542901A (en) | 1895-07-16 | Cabinet | ||
US4535060A (en) | 1983-01-05 | 1985-08-13 | Calgene, Inc. | Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthetase, production and use |
US5304732A (en) | 1984-03-06 | 1994-04-19 | Mgi Pharma, Inc. | Herbicide resistance in plants |
US4761373A (en) | 1984-03-06 | 1988-08-02 | Molecular Genetics, Inc. | Herbicide resistance in plants |
US5331107A (en) | 1984-03-06 | 1994-07-19 | Mgi Pharma, Inc. | Herbicide resistance in plants |
US4945050A (en) | 1984-11-13 | 1990-07-31 | Cornell Research Foundation, Inc. | Method for transporting substances into living cells and tissues and apparatus therefor |
DE3587548T2 (de) | 1984-12-28 | 1993-12-23 | Bayer Ag | Rekombinante DNA, die in pflanzliche Zellen eingebracht werden kann. |
US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US5569597A (en) | 1985-05-13 | 1996-10-29 | Ciba Geigy Corp. | Methods of inserting viral DNA into plant material |
US4940835A (en) | 1985-10-29 | 1990-07-10 | Monsanto Company | Glyphosate-resistant plants |
CA1313830C (en) | 1985-08-07 | 1993-02-23 | Dilip Maganlal Shah | Glyphosate-resistant plants |
ES2018274T5 (es) | 1986-03-11 | 1996-12-16 | Plant Genetic Systems Nv | Celulas vegetales resistentes a los inhibidores de glutamina sintetasa, preparadas por ingenieria genetica. |
US5187073A (en) | 1986-06-30 | 1993-02-16 | The University Of Toledo | Process for transforming gramineae and the products thereof |
US5273894A (en) | 1986-08-23 | 1993-12-28 | Hoechst Aktiengesellschaft | Phosphinothricin-resistance gene, and its use |
US5013659A (en) | 1987-07-27 | 1991-05-07 | E. I. Du Pont De Nemours And Company | Nucleic acid fragment encoding herbicide resistant plant acetolactate synthase |
US5378824A (en) | 1986-08-26 | 1995-01-03 | E. I. Du Pont De Nemours And Company | Nucleic acid fragment encoding herbicide resistant plant acetolactate synthase |
US5605011A (en) | 1986-08-26 | 1997-02-25 | E. I. Du Pont De Nemours And Company | Nucleic acid fragment encoding herbicide resistant plant acetolactate synthase |
JPH02503263A (ja) | 1986-10-01 | 1990-10-11 | ザ プラント セル リサーチ インスティチュート,インコーポレイテッド | ククミス種植物のインビトロでの遺伝的形質転換および制御再生 |
US5268463A (en) | 1986-11-11 | 1993-12-07 | Jefferson Richard A | Plant promoter α-glucuronidase gene construct |
US5608142A (en) | 1986-12-03 | 1997-03-04 | Agracetus, Inc. | Insecticidal cotton plants |
US5145783A (en) | 1987-05-26 | 1992-09-08 | Monsanto Company | Glyphosate-tolerant 5-endolpyruvyl-3-phosphoshikimate synthase |
US4971908A (en) | 1987-05-26 | 1990-11-20 | Monsanto Company | Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate synthase |
US5312910A (en) | 1987-05-26 | 1994-05-17 | Monsanto Company | Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate synthase |
US5316931A (en) | 1988-02-26 | 1994-05-31 | Biosource Genetics Corp. | Plant viral vectors having heterologous subgenomic promoters for systemic expression of foreign genes |
US5990387A (en) | 1988-06-10 | 1999-11-23 | Pioneer Hi-Bred International, Inc. | Stable transformation of plant cells |
US6015891A (en) | 1988-09-09 | 2000-01-18 | Mycogen Plant Science, Inc. | Synthetic insecticidal crystal protein gene having a modified frequency of codon usage |
US5023179A (en) | 1988-11-14 | 1991-06-11 | Eric Lam | Promoter enhancer element for gene expression in plant roots |
US5110732A (en) | 1989-03-14 | 1992-05-05 | The Rockefeller University | Selective gene expression in plants |
US5240855A (en) | 1989-05-12 | 1993-08-31 | Pioneer Hi-Bred International, Inc. | Particle gun |
US5879918A (en) | 1989-05-12 | 1999-03-09 | Pioneer Hi-Bred International, Inc. | Pretreatment of microprojectiles prior to using in a particle gun |
US5310667A (en) | 1989-07-17 | 1994-05-10 | Monsanto Company | Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate synthases |
US5550318A (en) | 1990-04-17 | 1996-08-27 | Dekalb Genetics Corporation | Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof |
US5322783A (en) | 1989-10-17 | 1994-06-21 | Pioneer Hi-Bred International, Inc. | Soybean transformation by microparticle bombardment |
ATE126535T1 (de) | 1990-04-05 | 1995-09-15 | Roberto Crea | ''walk-through''-mutagenese. |
ES2187497T3 (es) | 1990-04-12 | 2003-06-16 | Syngenta Participations Ag | Promotores preferentemente en tejidos. |
WO1991016433A1 (en) | 1990-04-26 | 1991-10-31 | Plant Genetic Systems N.V. | New bacillus thuringiensis strains and their genes encoding insecticidal toxins |
US5498830A (en) | 1990-06-18 | 1996-03-12 | Monsanto Company | Decreased oil content in plant seeds |
DK0536330T3 (da) | 1990-06-25 | 2002-04-22 | Monsanto Technology Llc | Glyphosattolerante planter |
US5633435A (en) | 1990-08-31 | 1997-05-27 | Monsanto Company | Glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthases |
US5866775A (en) | 1990-09-28 | 1999-02-02 | Monsanto Company | Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate synthases |
US5932782A (en) | 1990-11-14 | 1999-08-03 | Pioneer Hi-Bred International, Inc. | Plant transformation method using agrobacterium species adhered to microprojectiles |
US5277905A (en) | 1991-01-16 | 1994-01-11 | Mycogen Corporation | Coleopteran-active bacillus thuringiensis isolate |
US5459252A (en) | 1991-01-31 | 1995-10-17 | North Carolina State University | Root specific gene promoter |
FR2673642B1 (fr) | 1991-03-05 | 1994-08-12 | Rhone Poulenc Agrochimie | Gene chimere comprenant un promoteur capable de conferer a une plante une tolerance accrue au glyphosate. |
FR2673643B1 (fr) | 1991-03-05 | 1993-05-21 | Rhone Poulenc Agrochimie | Peptide de transit pour l'insertion d'un gene etranger dans un gene vegetal et plantes transformees en utilisant ce peptide. |
USRE36449E (en) | 1991-03-05 | 1999-12-14 | Rhone-Poulenc Agro | Chimeric gene for the transformation of plants |
US5399680A (en) | 1991-05-22 | 1995-03-21 | The Salk Institute For Biological Studies | Rice chitinase promoter |
US5731180A (en) | 1991-07-31 | 1998-03-24 | American Cyanamid Company | Imidazolinone resistant AHAS mutants |
ATE174626T1 (de) | 1991-08-02 | 1999-01-15 | Mycogen Corp | Neuer mikroorganismus und insektizid |
ES2140416T3 (es) | 1991-08-27 | 2000-03-01 | Novartis Ag | Proteinas con propiedades insecticidas contra insectos homopteros y su uso en la proteccion de plantas. |
EP0612208B1 (en) | 1991-10-04 | 2004-09-15 | North Carolina State University | Pathogen-resistant transgenic plants |
US5952544A (en) | 1991-12-04 | 1999-09-14 | E. I. Du Pont De Nemours And Company | Fatty acid desaturase genes from plants |
US5324646A (en) | 1992-01-06 | 1994-06-28 | Pioneer Hi-Bred International, Inc. | Methods of regeneration of Medicago sativa and expressing foreign DNA in same |
US5341001A (en) | 1992-02-13 | 1994-08-23 | Matsushita Electric Industrial Co., Ltd. | Sulfide-selenide manganese-zinc mixed crystal photo semiconductor and laser diode |
EP0604662B1 (en) | 1992-07-07 | 2008-06-18 | Japan Tobacco Inc. | Method of transforming monocotyledon |
US5401836A (en) | 1992-07-16 | 1995-03-28 | Pioneer Hi-Bre International, Inc. | Brassica regulatory sequence for root-specific or root-abundant gene expression |
AU670316B2 (en) | 1992-07-27 | 1996-07-11 | Pioneer Hi-Bred International, Inc. | An improved method of (agrobacterium)-mediated transformation of cultured soybean cells |
WO1994011516A1 (en) | 1992-11-17 | 1994-05-26 | E.I. Du Pont De Nemours And Company | Genes for microsomal delta-12 fatty acid desaturases and related enzymes from plants |
EP0625006A4 (en) | 1992-11-20 | 1996-04-24 | Agracetus | Transgenic cotton plants producing heterologous bioplastic. |
IL108241A (en) | 1992-12-30 | 2000-08-13 | Biosource Genetics Corp | Plant expression system comprising a defective tobamovirus replicon integrated into the plant chromosome and a helper virus |
ATE205533T1 (de) | 1993-01-13 | 2001-09-15 | Pioneer Hi Bred Int | Derivate von alpha-hordothionin mit höherem behalt an lysin |
US5583210A (en) | 1993-03-18 | 1996-12-10 | Pioneer Hi-Bred International, Inc. | Methods and compositions for controlling plant development |
US5814618A (en) | 1993-06-14 | 1998-09-29 | Basf Aktiengesellschaft | Methods for regulating gene expression |
US5789156A (en) | 1993-06-14 | 1998-08-04 | Basf Ag | Tetracycline-regulated transcriptional inhibitors |
US5426039A (en) | 1993-09-08 | 1995-06-20 | Bio-Rad Laboratories, Inc. | Direct molecular cloning of primer extended DNA containing an alkane diol |
US5470353A (en) | 1993-10-20 | 1995-11-28 | Hollister Incorporated | Post-operative thermal blanket |
JPH07177130A (ja) | 1993-12-21 | 1995-07-14 | Fujitsu Ltd | エラーカウント回路 |
US5834252A (en) | 1995-04-18 | 1998-11-10 | Glaxo Group Limited | End-complementary polymerase reaction |
US6335160B1 (en) | 1995-02-17 | 2002-01-01 | Maxygen, Inc. | Methods and compositions for polypeptide engineering |
US6117679A (en) | 1994-02-17 | 2000-09-12 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
US5837458A (en) | 1994-02-17 | 1998-11-17 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
US5593881A (en) | 1994-05-06 | 1997-01-14 | Mycogen Corporation | Bacillus thuringiensis delta-endotoxin |
US5767373A (en) | 1994-06-16 | 1998-06-16 | Novartis Finance Corporation | Manipulation of protoporphyrinogen oxidase enzyme activity in eukaryotic organisms |
US5736369A (en) | 1994-07-29 | 1998-04-07 | Pioneer Hi-Bred International, Inc. | Method for producing transgenic cereal plants |
US5792931A (en) | 1994-08-12 | 1998-08-11 | Pioneer Hi-Bred International, Inc. | Fumonisin detoxification compositions and methods |
US5608144A (en) | 1994-08-12 | 1997-03-04 | Dna Plant Technology Corp. | Plant group 2 promoters and uses thereof |
US5750868A (en) | 1994-12-08 | 1998-05-12 | Pioneer Hi-Bred International, Inc. | Reversible nuclear genetic system for male sterility in transgenic plants |
US5659026A (en) | 1995-03-24 | 1997-08-19 | Pioneer Hi-Bred International | ALS3 promoter |
US5853973A (en) | 1995-04-20 | 1998-12-29 | American Cyanamid Company | Structure based designed herbicide resistant products |
CZ331797A3 (cs) | 1995-04-20 | 1998-06-17 | American Cyanamid Company | Produkty rezistentní na herbicidy vyvíjené na struktuře založeným způsobem |
WO1996034112A1 (en) | 1995-04-24 | 1996-10-31 | Chromaxome Corp. | Methods for generating and screening novel metabolic pathways |
FR2734842B1 (fr) | 1995-06-02 | 1998-02-27 | Rhone Poulenc Agrochimie | Sequence adn d'un gene de l'hydroxy-phenyl pyruvate dioxygenase et obtention de plantes contenant un gene de l'hydroxy-phenyl pyruvate dioxygenase, tolerantes a certains herbicides |
EP0828835A1 (en) | 1995-06-02 | 1998-03-18 | Pioneer Hi-Bred International, Inc. | HIGH THREONINE DERIVATIVES OF $g(a)-HORDOTHIONIN |
EP0832235A1 (en) | 1995-06-02 | 1998-04-01 | Pioneer Hi-Bred International, Inc. | HIGH METHIONINE DERIVATIVES OF alfa-HORDOTHIONIN |
US6057103A (en) | 1995-07-18 | 2000-05-02 | Diversa Corporation | Screening for novel bioactivities |
US5958672A (en) | 1995-07-18 | 1999-09-28 | Diversa Corporation | Protein activity screening of clones having DNA from uncultivated microorganisms |
FR2736926B1 (fr) | 1995-07-19 | 1997-08-22 | Rhone Poulenc Agrochimie | 5-enol pyruvylshikimate-3-phosphate synthase mutee, gene codant pour cette proteine et plantes transformees contenant ce gene |
US5837876A (en) | 1995-07-28 | 1998-11-17 | North Carolina State University | Root cortex specific gene promoter |
US5633636A (en) | 1995-10-02 | 1997-05-27 | Analog Devices, Inc. | Half-gray digital encoding method and circuitry |
US5756316A (en) | 1995-11-02 | 1998-05-26 | Genencor International, Inc. | Molecular cloning by multimerization of plasmids |
US5981722A (en) | 1995-11-20 | 1999-11-09 | Board Of Regents For The University Of Oklahoma | Trypsin inhibitors with insecticidal properties obtained from PENTACLETHRA MACROLOBA |
US5737514A (en) | 1995-11-29 | 1998-04-07 | Texas Micro, Inc. | Remote checkpoint memory system and protocol for fault-tolerant computer system |
US5939250A (en) | 1995-12-07 | 1999-08-17 | Diversa Corporation | Production of enzymes having desired activities by mutagenesis |
US5965408A (en) | 1996-07-09 | 1999-10-12 | Diversa Corporation | Method of DNA reassembly by interrupting synthesis |
US20030215798A1 (en) | 1997-06-16 | 2003-11-20 | Diversa Corporation | High throughput fluorescence-based screening for novel enzymes |
US6171820B1 (en) | 1995-12-07 | 2001-01-09 | Diversa Corporation | Saturation mutagenesis in directed evolution |
US6096548A (en) | 1996-03-25 | 2000-08-01 | Maxygen, Inc. | Method for directing evolution of a virus |
US5783431A (en) | 1996-04-24 | 1998-07-21 | Chromaxome Corporation | Methods for generating and screening novel metabolic pathways |
US6072050A (en) | 1996-06-11 | 2000-06-06 | Pioneer Hi-Bred International, Inc. | Synthetic promoters |
JP2001504325A (ja) | 1996-09-27 | 2001-04-03 | マキシジェン,インコーポレイテッド | 回帰的配列シャフリングおよび選択による遺伝子治療の最適化のための方法 |
JP3441899B2 (ja) | 1996-11-01 | 2003-09-02 | 理化学研究所 | 完全長cDNAライブラリーの作成方法 |
US6232529B1 (en) | 1996-11-20 | 2001-05-15 | Pioneer Hi-Bred International, Inc. | Methods of producing high-oil seed by modification of starch levels |
DE19652284A1 (de) | 1996-12-16 | 1998-06-18 | Hoechst Schering Agrevo Gmbh | Neue Gene codierend für Aminosäure-Deacetylasen mit Spezifität für N-Acetyl-L-Phosphinothricin, ihre Isolierung und Verwendung |
EP2261373A3 (en) | 1997-01-17 | 2011-12-14 | Codexis Mayflower Holdings, LLC | Evolution of whole cells and organisms by recursive sequence recombination |
US6326204B1 (en) | 1997-01-17 | 2001-12-04 | Maxygen, Inc. | Evolution of whole cells and organisms by recursive sequence recombination |
US5981840A (en) | 1997-01-24 | 1999-11-09 | Pioneer Hi-Bred International, Inc. | Methods for agrobacterium-mediated transformation |
JP4263248B2 (ja) | 1997-03-18 | 2009-05-13 | ノボザイムス アクティーゼルスカブ | Dnaのシャッフリングによるライブラリーの作成方法 |
NZ337900A (en) | 1997-03-18 | 2001-05-25 | Novo Nordisk As | DNA shuffling for randomised heterologous and homogenous 3' and 5' primers for construction of DNA libraries for generation of more desirable mutants |
US5948653A (en) | 1997-03-21 | 1999-09-07 | Pati; Sushma | Sequence alterations using homologous recombination |
US6153410A (en) | 1997-03-25 | 2000-11-28 | California Institute Of Technology | Recombination of polynucleotide sequences using random or defined primers |
US6399383B1 (en) | 1997-10-28 | 2002-06-04 | Maxygen, Inc. | Human papilloma virus vectors |
US6596539B1 (en) | 1997-10-31 | 2003-07-22 | Maxygen, Inc. | Modification of virus tropism and host range by viral genome shuffling |
US6245968B1 (en) | 1997-11-07 | 2001-06-12 | Aventis Cropscience S.A. | Mutated hydroxyphenylpyruvate dioxygenase, DNA sequence and isolation of plants which contain such a gene and which are tolerant to herbicides |
US6069115A (en) | 1997-11-12 | 2000-05-30 | Rhone-Poulenc Agrochimie | Method of controlling weeds in transgenic crops |
CA2306053C (en) | 1997-11-18 | 2003-01-21 | Pioneer Hi-Bred International, Inc. | Mobilization of viral genomes from t-dna using site-specific recombination systems |
BR9814669A (pt) | 1997-11-18 | 2001-11-20 | Pioneer Hi Bred Int | Método para a integração de dna estranho emgenomas eucarióticos |
EP1574573B9 (en) | 1997-11-18 | 2009-03-04 | Pioneer Hi-Bred International, Inc. | Compositions and methods for genetic modification of plants |
WO1999025853A1 (en) | 1997-11-18 | 1999-05-27 | Pioneer Hi-Bred International, Inc. | Targeted manipulation of herbicide-resistance genes in plants |
EP2270234B1 (en) | 1997-12-08 | 2013-03-06 | California Institute of Technology | Method for creating polynucleotide and polypeptide sequences |
JP2002503461A (ja) | 1998-02-11 | 2002-02-05 | マキシジェン, インコーポレイテッド | 遺伝子ワクチンベクター工学 |
WO1999041383A1 (en) | 1998-02-11 | 1999-08-19 | Maxygen, Inc. | Antigen library immunization |
AU762993C (en) | 1998-02-26 | 2004-06-10 | Pioneer Hi-Bred International, Inc. | Constitutive maize promoters |
US5955310A (en) | 1998-02-26 | 1999-09-21 | Novo Nordisk Biotech, Inc. | Methods for producing a polypeptide in a bacillus cell |
EP1080197A2 (en) | 1998-05-22 | 2001-03-07 | Pioneer Hi-Bred International, Inc. | Cell cycle genes, proteins and uses thereof |
WO2000000632A1 (en) | 1998-06-29 | 2000-01-06 | Phylos, Inc. | Methods for generating highly diverse libraries |
WO2000009727A2 (en) | 1998-08-12 | 2000-02-24 | Maxygen, Inc. | Dna shuffling to produce herbicide selective crops |
FR2782323B1 (fr) | 1998-08-12 | 2002-01-11 | Proteus | Procede de production in vitro de sequences polynucleotidiques recombinees, banques de sequences et sequences ainsi obtenues |
ATE309362T1 (de) | 1998-08-20 | 2005-11-15 | Pioneer Hi Bred Int | Samen-bevorzugende promotoren |
US5981849A (en) | 1998-08-27 | 1999-11-09 | Monsanto Corporation | Soybean cultivar 95-060911 |
WO2000012733A1 (en) | 1998-08-28 | 2000-03-09 | Pioneer Hi-Bred International, Inc. | Seed-preferred promoters from end genes |
US6518487B1 (en) | 1998-09-23 | 2003-02-11 | Pioneer Hi-Bred International, Inc. | Cyclin D polynucleotides, polypeptides and uses thereof |
KR20010085850A (ko) | 1998-09-29 | 2001-09-07 | 추후제출 | 코돈 변형 유전자의 재편성 |
IL142736A0 (en) | 1998-11-09 | 2002-03-10 | Pioneer Hi Bred Int | Transcriptional activator lec 1 nucleic acids, polypeptides and their uses |
DE69941009D1 (de) | 1998-11-17 | 2009-07-30 | Monsanto Technology Llc | Phosphonat metabolisierende pflanzen |
US7217858B2 (en) | 1998-12-21 | 2007-05-15 | E. I. Du Pont De Nemours And Company | S-adenosyl-L-methionine synthetase promoter and its use in expression of transgenic genes in plants |
US6376246B1 (en) | 1999-02-05 | 2002-04-23 | Maxygen, Inc. | Oligonucleotide mediated nucleic acid recombination |
US6436675B1 (en) | 1999-09-28 | 2002-08-20 | Maxygen, Inc. | Use of codon-varied oligonucleotide synthesis for synthetic shuffling |
WO2000042559A1 (en) | 1999-01-18 | 2000-07-20 | Maxygen, Inc. | Methods of populating data structures for use in evolutionary simulations |
AU3210100A (en) | 1999-01-19 | 2000-08-01 | Maxygen, Inc. | Methods for making character strings, polynucleotides and polypeptides having desired characteristics |
AU4133100A (en) | 1999-04-29 | 2000-11-17 | Syngenta Limited | Herbicide resistant plants |
ES2262514T3 (es) * | 1999-04-29 | 2006-12-01 | Syngenta Limited | Plantas resistentes a herbicidas. |
HUP0201018A2 (en) | 1999-04-29 | 2002-07-29 | Syngenta Ltd | Herbicide resistant plants |
EP1200611A1 (en) | 1999-08-13 | 2002-05-02 | Syngenta Participations AG | Herbicide-tolerant protoporphyrinogen oxidase |
AU2001241939A1 (en) | 2000-02-28 | 2001-09-12 | Maxygen, Inc. | Single-stranded nucleic acid template-mediated recombination and nucleic acid fragment isolation |
AU2001242005B2 (en) | 2000-03-09 | 2006-04-27 | Monsanto Technology Llc | Methods for making plants tolerant to glyphosate and compositions thereof |
IL155599A0 (en) * | 2000-10-30 | 2003-11-23 | Maxygen Inc | Polynucleotides encoding proteins which catalyze acetylation of glyphosate |
AU2003234328A1 (en) * | 2002-04-30 | 2003-11-17 | Pioneer Hi-Bred International, Inc. | Novel glyphosate-n-acetyltransferase (gat) genes |
FR2948775B1 (fr) | 2009-07-31 | 2011-12-02 | Horiba Jobin Yvon Sas | Systeme optique planaire d'imagerie polychromatique a large champ de vision |
US10470602B2 (en) | 2011-06-13 | 2019-11-12 | National Presto Industries, Inc. | Pump coffee brewer |
US9823098B2 (en) | 2014-05-05 | 2017-11-21 | Filippo Bastianini | Apparatus for interrogating distributed optical fibre sensors using a stimulated brillouin scattering optical frequency-domain interferometer |
CN105578337A (zh) | 2014-10-07 | 2016-05-11 | 鸿富锦精密工业(深圳)有限公司 | 共振音响 |
-
2004
- 2004-04-29 EP EP11153914A patent/EP2322629A3/en not_active Withdrawn
- 2004-04-29 EP EP12179273.3A patent/EP2535414B1/en not_active Expired - Lifetime
- 2004-04-29 BR BRPI0409816A patent/BRPI0409816B8/pt active IP Right Grant
- 2004-04-29 CN CN2004800183990A patent/CN1863914B/zh not_active Expired - Lifetime
- 2004-04-29 CA CA2662092A patent/CA2662092C/en not_active Expired - Lifetime
- 2004-04-29 MX MXPA05011585A patent/MXPA05011585A/es active IP Right Grant
- 2004-04-29 CA CA2521284A patent/CA2521284C/en not_active Expired - Lifetime
- 2004-04-29 JP JP2006532493A patent/JP2007500514A/ja active Pending
- 2004-04-29 SG SG200705305-1A patent/SG155063A1/en unknown
- 2004-04-29 WO PCT/US2004/013145 patent/WO2005012515A2/en active Application Filing
- 2004-04-29 EP EP04775913A patent/EP1620557A2/en not_active Withdrawn
- 2004-04-29 AU AU2004260931A patent/AU2004260931B9/en not_active Expired
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2005
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Also Published As
Publication number | Publication date |
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CA2662092A1 (en) | 2005-02-10 |
BRPI0409816B8 (pt) | 2022-12-06 |
MXPA05011585A (es) | 2006-05-25 |
SG155063A1 (en) | 2009-09-30 |
ZA200509602B (en) | 2007-04-25 |
AU2004260931A1 (en) | 2005-02-10 |
EP2322629A2 (en) | 2011-05-18 |
EP2535414B1 (en) | 2017-12-13 |
EP2535414A8 (en) | 2013-04-03 |
BRPI0409816B1 (pt) | 2019-12-17 |
AU2004260931B2 (en) | 2009-11-19 |
WO2005012515A3 (en) | 2005-11-17 |
BRPI0409816A (pt) | 2006-05-02 |
EP2322629A3 (en) | 2011-11-02 |
JP2007500514A (ja) | 2007-01-18 |
AU2004260931B9 (en) | 2012-01-19 |
CA2521284C (en) | 2014-07-08 |
CA2662092C (en) | 2012-07-17 |
HRP20050930A2 (en) | 2006-03-31 |
CN1863914B (zh) | 2011-03-09 |
CA2521284A1 (en) | 2005-02-10 |
EP2535414A1 (en) | 2012-12-19 |
WO2005012515A8 (en) | 2005-05-12 |
EP1620557A2 (en) | 2006-02-01 |
WO2005012515A2 (en) | 2005-02-10 |
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