CN114410625B - 通过Cas9-crRNA复合物的RNA指导的DNA裂解 - Google Patents
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Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN202111462778.0A CN114410625B (zh) | 2012-03-20 | 2013-03-20 | 通过Cas9-crRNA复合物的RNA指导的DNA裂解 |
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| US201261613373P | 2012-03-20 | 2012-03-20 | |
| US61/613,373 | 2012-03-20 | ||
| US201261625420P | 2012-04-17 | 2012-04-17 | |
| US61/625,420 | 2012-04-17 | ||
| CN201380023255.3A CN104520429B (zh) | 2012-03-20 | 2013-03-20 | 通过Cas9-crRNA复合物的RNA指导的DNA裂解 |
| CN202111462778.0A CN114410625B (zh) | 2012-03-20 | 2013-03-20 | 通过Cas9-crRNA复合物的RNA指导的DNA裂解 |
| PCT/US2013/033106 WO2013142578A1 (en) | 2012-03-20 | 2013-03-20 | RNA-DIRECTED DNA CLEAVAGE BY THE Cas9-crRNA COMPLEX |
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| CN201380023255.3A Division CN104520429B (zh) | 2012-03-20 | 2013-03-20 | 通过Cas9-crRNA复合物的RNA指导的DNA裂解 |
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| CN114410625A CN114410625A (zh) | 2022-04-29 |
| CN114410625B true CN114410625B (zh) | 2024-06-25 |
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Families Citing this family (437)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR112012028805A2 (pt) | 2010-05-10 | 2019-09-24 | The Regents Of The Univ Of California E Nereus Pharmaceuticals Inc | composições de endorribonuclease e métodos de uso das mesmas. |
| US10687975B2 (en) | 2011-02-04 | 2020-06-23 | Joseph E. Kovarik | Method and system to facilitate the growth of desired bacteria in a human's mouth |
| US11951139B2 (en) | 2015-11-30 | 2024-04-09 | Seed Health, Inc. | Method and system for reducing the likelihood of osteoporosis |
| US12279989B2 (en) | 2011-02-04 | 2025-04-22 | Seed Health, Inc. | Method and system for increasing beneficial bacteria and decreasing pathogenic bacteria in the oral cavity |
| US12533312B2 (en) | 2011-02-04 | 2026-01-27 | Seed Health, Inc. | Method and system for preventing sore throat in humans |
| US11523934B2 (en) | 2011-02-04 | 2022-12-13 | Seed Health, Inc. | Method and system to facilitate the growth of desired bacteria in a human's mouth |
| US11844720B2 (en) | 2011-02-04 | 2023-12-19 | Seed Health, Inc. | Method and system to reduce the likelihood of dental caries and halitosis |
| US11951140B2 (en) | 2011-02-04 | 2024-04-09 | Seed Health, Inc. | Modulation of an individual's gut microbiome to address osteoporosis and bone disease |
| US12257272B2 (en) | 2015-12-24 | 2025-03-25 | Seed Health, Inc. | Method and system for reducing the likelihood of developing depression in an individual |
| US10583033B2 (en) | 2011-02-04 | 2020-03-10 | Katherine Rose Kovarik | Method and system for reducing the likelihood of a porphyromonas gingivalis infection in a human being |
| US11191665B2 (en) | 2011-02-04 | 2021-12-07 | Joseph E. Kovarik | Method and system for reducing the likelihood of a porphyromonas gingivalis infection in a human being |
| US11998479B2 (en) | 2011-02-04 | 2024-06-04 | Seed Health, Inc. | Method and system for addressing adverse effects on the oral microbiome and restoring gingival health caused by sodium lauryl sulphate exposure |
| BR112013024337A2 (pt) | 2011-03-23 | 2017-09-26 | Du Pont | locus de traço transgênico complexo em uma planta, planta ou semente, método para produzir em uma planta um locus de traço transgênico complexo e construto de expressão |
| EP2734621B1 (en) | 2011-07-22 | 2019-09-04 | President and Fellows of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| EP3604555B1 (en) | 2011-10-14 | 2024-12-25 | President and Fellows of Harvard College | Sequencing by structure assembly |
| WO2014163886A1 (en) | 2013-03-12 | 2014-10-09 | President And Fellows Of Harvard College | Method of generating a three-dimensional nucleic acid containing matrix |
| ES2991004T3 (es) | 2011-12-22 | 2024-12-02 | Harvard College | Métodos para la detección de analitos |
| GB201122458D0 (en) | 2011-12-30 | 2012-02-08 | Univ Wageningen | Modified cascade ribonucleoproteins and uses thereof |
| US9637739B2 (en) | 2012-03-20 | 2017-05-02 | Vilnius University | RNA-directed DNA cleavage by the Cas9-crRNA complex |
| CN104364380B (zh) | 2012-04-25 | 2018-10-09 | 瑞泽恩制药公司 | 核酸酶介导的使用大靶向载体的靶向 |
| PL4289948T3 (pl) * | 2012-05-25 | 2025-06-02 | The Regents Of The University Of California | Sposoby i kompozycje do modyfikacji kierowanego na rna docelowego dna i do nakierowanej na rna modulacji transkrypcji |
| KR20230065381A (ko) * | 2012-07-25 | 2023-05-11 | 더 브로드 인스티튜트, 인코퍼레이티드 | 유도 dna 결합 단백질 및 게놈 교란 도구 및 이의 적용 |
| EP2912175B1 (en) | 2012-10-23 | 2018-08-22 | Toolgen Incorporated | Composition for cleaving a target dna comprising a guide rna specific for the target dna and cas protein-encoding nucleic acid or cas protein, and use thereof |
| CN105142669B (zh) | 2012-12-06 | 2018-07-03 | 西格马-奥尔德里奇有限责任公司 | 基于crispr的基因组修饰和调控 |
| WO2014093701A1 (en) | 2012-12-12 | 2014-06-19 | The Broad Institute, Inc. | Functional genomics using crispr-cas systems, compositions, methods, knock out libraries and applications thereof |
| EP3434776A1 (en) | 2012-12-12 | 2019-01-30 | The Broad Institute, Inc. | Methods, models, systems, and apparatus for identifying target sequences for cas enzymes or crispr-cas systems for target sequences and conveying results thereof |
| US8697359B1 (en) * | 2012-12-12 | 2014-04-15 | The Broad Institute, Inc. | CRISPR-Cas systems and methods for altering expression of gene products |
| WO2014093694A1 (en) * | 2012-12-12 | 2014-06-19 | The Broad Institute, Inc. | Crispr-cas nickase systems, methods and compositions for sequence manipulation in eukaryotes |
| WO2014093655A2 (en) * | 2012-12-12 | 2014-06-19 | The Broad Institute, Inc. | Engineering and optimization of systems, methods and compositions for sequence manipulation with functional domains |
| PT2898075E (pt) | 2012-12-12 | 2016-06-16 | Harvard College | Manipulação e otimização de sistemas, métodos e composições de enzima melhorados para manipulação de sequências |
| CN105121648B (zh) | 2012-12-12 | 2021-05-07 | 布罗德研究所有限公司 | 用于序列操纵的系统、方法和优化的指导组合物的工程化 |
| CN105658796B (zh) | 2012-12-12 | 2021-10-26 | 布罗德研究所有限公司 | 用于序列操纵的crispr-cas组分系统、方法以及组合物 |
| WO2014093622A2 (en) | 2012-12-12 | 2014-06-19 | The Broad Institute, Inc. | Delivery, engineering and optimization of systems, methods and compositions for sequence manipulation and therapeutic applications |
| EP4282970A3 (en) * | 2012-12-17 | 2024-01-17 | President and Fellows of Harvard College | Rna-guided human genome engineering |
| AU2014207618A1 (en) | 2013-01-16 | 2015-08-06 | Emory University | Cas9-nucleic acid complexes and uses related thereto |
| KR20170108172A (ko) | 2013-03-14 | 2017-09-26 | 카리부 바이오사이언시스 인코포레이티드 | 핵산-표적화 핵산의 조성물 및 방법 |
| US10760064B2 (en) | 2013-03-15 | 2020-09-01 | The General Hospital Corporation | RNA-guided targeting of genetic and epigenomic regulatory proteins to specific genomic loci |
| IL289396B2 (en) | 2013-03-15 | 2023-12-01 | The General Hospital Coporation | Using truncated guide rnas (tru-grnas) to increase specificity for rna-guided genome editing |
| US9902973B2 (en) | 2013-04-11 | 2018-02-27 | Caribou Biosciences, Inc. | Methods of modifying a target nucleic acid with an argonaute |
| CA2908697C (en) | 2013-04-16 | 2023-12-12 | Regeneron Pharmaceuticals, Inc. | Targeted modification of rat genome |
| JP7065564B2 (ja) * | 2013-05-29 | 2022-05-12 | セレクティス | Cas9ニッカーゼ活性を用いて正確なdna切断をもたらすための方法 |
| US9873907B2 (en) | 2013-05-29 | 2018-01-23 | Agilent Technologies, Inc. | Method for fragmenting genomic DNA using CAS9 |
| US20140356956A1 (en) | 2013-06-04 | 2014-12-04 | President And Fellows Of Harvard College | RNA-Guided Transcriptional Regulation |
| MY197877A (en) | 2013-06-04 | 2023-07-22 | Harvard College | Rna-guided transcriptional regulation |
| EP3597755A1 (en) | 2013-06-17 | 2020-01-22 | The Broad Institute, Inc. | Delivery, use and therapeutic applications of the crispr-cas systems and compositions for targeting disorders and diseases using viral components |
| EP3674411A1 (en) * | 2013-06-17 | 2020-07-01 | The Broad Institute, Inc. | Delivery, engineering and optimization of tandem guide systems, methods and compositions for sequence manipulation |
| EP3011033B1 (en) | 2013-06-17 | 2020-02-19 | The Broad Institute, Inc. | Functional genomics using crispr-cas systems, compositions methods, screens and applications thereof |
| KR20240172759A (ko) | 2013-06-17 | 2024-12-10 | 더 브로드 인스티튜트, 인코퍼레이티드 | 간의 표적화 및 치료를 위한 CRISPRCas 시스템, 벡터 및 조성물의 전달 및 용도 |
| CA2915837A1 (en) | 2013-06-17 | 2014-12-24 | The Broad Institute, Inc. | Optimized crispr-cas double nickase systems, methods and compositions for sequence manipulation |
| US10011850B2 (en) | 2013-06-21 | 2018-07-03 | The General Hospital Corporation | Using RNA-guided FokI Nucleases (RFNs) to increase specificity for RNA-Guided Genome Editing |
| AU2014287397B2 (en) | 2013-07-10 | 2019-10-10 | President And Fellows Of Harvard College | Orthogonal Cas9 proteins for RNA-guided gene regulation and editing |
| US11306328B2 (en) | 2013-07-26 | 2022-04-19 | President And Fellows Of Harvard College | Genome engineering |
| US10421957B2 (en) | 2013-07-29 | 2019-09-24 | Agilent Technologies, Inc. | DNA assembly using an RNA-programmable nickase |
| US9163284B2 (en) | 2013-08-09 | 2015-10-20 | President And Fellows Of Harvard College | Methods for identifying a target site of a Cas9 nuclease |
| US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
| MX363842B (es) | 2013-08-22 | 2019-04-05 | Du Pont | Métodos para producir modificaciones genéticas en un genoma vegetal sin incorporar un marcador transgénico seleccionable, y composiciones de éstas. |
| US9340800B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | Extended DNA-sensing GRNAS |
| US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
| US9526784B2 (en) * | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
| EP3418379B1 (en) | 2013-09-18 | 2020-12-09 | Kymab Limited | Methods, cells & organisms |
| WO2015065964A1 (en) | 2013-10-28 | 2015-05-07 | The Broad Institute Inc. | Functional genomics using crispr-cas systems, compositions, methods, screens and applications thereof |
| US10584358B2 (en) | 2013-10-30 | 2020-03-10 | North Carolina State University | Compositions and methods related to a type-II CRISPR-Cas system in Lactobacillus buchneri |
| JP2016536021A (ja) | 2013-11-07 | 2016-11-24 | エディタス・メディシン,インコーポレイテッド | CRISPR関連方法および支配gRNAのある組成物 |
| WO2015070193A1 (en) * | 2013-11-11 | 2015-05-14 | Liu Oliver | Compositions and methods for targeted gene disruption in prokaryotes |
| WO2015075056A1 (en) * | 2013-11-19 | 2015-05-28 | Thermo Fisher Scientific Baltics Uab | Programmable enzymes for isolation of specific dna fragments |
| US10787684B2 (en) * | 2013-11-19 | 2020-09-29 | President And Fellows Of Harvard College | Large gene excision and insertion |
| US9074199B1 (en) | 2013-11-19 | 2015-07-07 | President And Fellows Of Harvard College | Mutant Cas9 proteins |
| US10557151B2 (en) | 2013-11-28 | 2020-02-11 | Horizon Discovery Limited | Somatic human cell line mutations |
| SG10201700961TA (en) | 2013-12-11 | 2017-04-27 | Regeneron Pharma | Methods and compositions for the targeted modification of a genome |
| HUE041331T2 (hu) | 2013-12-11 | 2019-05-28 | Regeneron Pharma | Módszerek és készítmények a genom célzott módosításához |
| EP3080271B1 (en) | 2013-12-12 | 2020-02-12 | The Broad Institute, Inc. | Systems, methods and compositions for sequence manipulation with optimized functional crispr-cas systems |
| EP3653229A1 (en) | 2013-12-12 | 2020-05-20 | The Broad Institute, Inc. | Delivery, use and therapeutic applications of the crispr-cas systems and compositions for genome editing |
| EP3080260B1 (en) | 2013-12-12 | 2019-03-06 | The Broad Institute, Inc. | Crispr-cas systems and methods for altering expression of gene products, structural information and inducible modular cas enzymes |
| WO2015089354A1 (en) | 2013-12-12 | 2015-06-18 | The Broad Institute Inc. | Compositions and methods of use of crispr-cas systems in nucleotide repeat disorders |
| US20150165054A1 (en) | 2013-12-12 | 2015-06-18 | President And Fellows Of Harvard College | Methods for correcting caspase-9 point mutations |
| WO2015089364A1 (en) | 2013-12-12 | 2015-06-18 | The Broad Institute Inc. | Crystal structure of a crispr-cas system, and uses thereof |
| EP3080259B1 (en) | 2013-12-12 | 2023-02-01 | The Broad Institute, Inc. | Engineering of systems, methods and optimized guide compositions with new architectures for sequence manipulation |
| CN105899658B (zh) | 2013-12-12 | 2020-02-18 | 布罗德研究所有限公司 | 针对hbv和病毒性疾病以及障碍的crispr-cas系统和组合物的递送、用途和治疗应用 |
| EP3083958B1 (en) | 2013-12-19 | 2019-04-17 | Amyris, Inc. | Methods for genomic integration |
| US12318377B2 (en) | 2013-12-20 | 2025-06-03 | Seed Health, Inc. | Method and system for reducing the likelihood of a porphyromonas gingivalis infection in a human being |
| US11969445B2 (en) | 2013-12-20 | 2024-04-30 | Seed Health, Inc. | Probiotic composition and method for controlling excess weight, obesity, NAFLD and NASH |
| US11826388B2 (en) | 2013-12-20 | 2023-11-28 | Seed Health, Inc. | Topical application of Lactobacillus crispatus to ameliorate barrier damage and inflammation |
| US12329783B2 (en) | 2013-12-20 | 2025-06-17 | Seed Health, Inc. | Method and system to improve the health of a person's skin microbiome |
| US11980643B2 (en) | 2013-12-20 | 2024-05-14 | Seed Health, Inc. | Method and system to modify an individual's gut-brain axis to provide neurocognitive protection |
| US12246043B2 (en) | 2013-12-20 | 2025-03-11 | Seed Health, Inc. | Topical application to treat acne vulgaris |
| US11998574B2 (en) | 2013-12-20 | 2024-06-04 | Seed Health, Inc. | Method and system for modulating an individual's skin microbiome |
| US11839632B2 (en) | 2013-12-20 | 2023-12-12 | Seed Health, Inc. | Topical application of CRISPR-modified bacteria to treat acne vulgaris |
| US11833177B2 (en) | 2013-12-20 | 2023-12-05 | Seed Health, Inc. | Probiotic to enhance an individual's skin microbiome |
| US11642382B2 (en) | 2013-12-20 | 2023-05-09 | Seed Health, Inc. | Method for treating an individual suffering from bladder cancer |
| US11672835B2 (en) | 2013-12-20 | 2023-06-13 | Seed Health, Inc. | Method for treating individuals having cancer and who are receiving cancer immunotherapy |
| US11529379B2 (en) | 2013-12-20 | 2022-12-20 | Seed Health, Inc. | Method and system for reducing the likelihood of developing colorectal cancer in an individual human being |
| US12005085B2 (en) | 2013-12-20 | 2024-06-11 | Seed Health, Inc. | Probiotic method and composition for maintaining a healthy vaginal microbiome |
| WO2015103153A1 (en) | 2013-12-31 | 2015-07-09 | The Regents Of The University Of California | Cas9 crystals and methods of use thereof |
| JP2017502683A (ja) * | 2014-01-14 | 2017-01-26 | ラム セラピューティクス, インコーポレイテッド | 変異誘発方法 |
| US10787654B2 (en) | 2014-01-24 | 2020-09-29 | North Carolina State University | Methods and compositions for sequence guiding Cas9 targeting |
| DE212015000061U1 (de) | 2014-02-11 | 2017-09-03 | The Regents Of The University Of Colorado, A Body Corporate | CRISPR-ermöglichtes Multiplex Genom Engineering |
| EP3105325B2 (en) | 2014-02-13 | 2024-10-23 | Takara Bio USA, Inc. | Methods of depleting a target molecule from an initial collection of nucleic acids, and compositions and kits for practicing the same |
| KR20160130392A (ko) | 2014-02-18 | 2016-11-11 | 듀크 유니버시티 | 바이러스 복제의 불활성화를 위한 조성물 및 그의 제조 및 사용 방법 |
| EP3957735A1 (en) | 2014-03-05 | 2022-02-23 | Editas Medicine, Inc. | Crispr/cas-related methods and compositions for treating usher syndrome and retinitis pigmentosa |
| ES2745769T3 (es) | 2014-03-10 | 2020-03-03 | Editas Medicine Inc | Procedimientos y composiciones relacionados con CRISPR/CAS para tratar la amaurosis congénita de Leber 10 (LCA10) |
| US11141493B2 (en) | 2014-03-10 | 2021-10-12 | Editas Medicine, Inc. | Compositions and methods for treating CEP290-associated disease |
| US11339437B2 (en) | 2014-03-10 | 2022-05-24 | Editas Medicine, Inc. | Compositions and methods for treating CEP290-associated disease |
| WO2015148863A2 (en) | 2014-03-26 | 2015-10-01 | Editas Medicine, Inc. | Crispr/cas-related methods and compositions for treating sickle cell disease |
| US12460231B2 (en) | 2014-04-02 | 2025-11-04 | Editas Medicine, Inc. | Crispr/CAS-related methods and compositions for treating primary open angle glaucoma |
| WO2015155686A2 (en) | 2014-04-08 | 2015-10-15 | North Carolina State University | Methods and compositions for rna-directed repression of transcription using crispr-associated genes |
| GB201406970D0 (en) | 2014-04-17 | 2014-06-04 | Green Biologics Ltd | Targeted mutations |
| GB201406968D0 (en) * | 2014-04-17 | 2014-06-04 | Green Biologics Ltd | Deletion mutants |
| JP2017516500A (ja) * | 2014-05-28 | 2017-06-22 | ツールゲン インコーポレイテッドToolgen Incorporated | 標的特異的ヌクレアーゼを用いた標的dnaの敏感な検出方法 |
| BR112016028023A2 (pt) | 2014-05-30 | 2017-08-22 | Univ Leland Stanford Junior | Composições e métodos de administração de tratamentos para infecções virais latentes |
| HUE049776T2 (hu) | 2014-06-06 | 2020-10-28 | Regeneron Pharma | Módszerek és készítmények egy célzott lókusz módosítására |
| MX384887B (es) * | 2014-06-23 | 2025-03-14 | Regeneron Pharma | Ensamblaje de adn mediado por nucleasa. |
| KR102425438B1 (ko) | 2014-06-23 | 2022-07-27 | 더 제너럴 하스피탈 코포레이션 | 서열결정에 의해 평가된 DSB의 게놈 전체에 걸친 비편향된 확인 (GUIDE-Seq) |
| EP3161128B1 (en) | 2014-06-26 | 2018-09-26 | Regeneron Pharmaceuticals, Inc. | Methods and compositions for targeted genetic modifications and methods of use |
| WO2016007347A1 (en) | 2014-07-11 | 2016-01-14 | E. I. Du Pont De Nemours And Company | Compositions and methods for producing plants resistant to glyphosate herbicide |
| WO2016007839A1 (en) | 2014-07-11 | 2016-01-14 | President And Fellows Of Harvard College | Methods for high-throughput labelling and detection of biological features in situ using microscopy |
| CA2956224A1 (en) | 2014-07-30 | 2016-02-11 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
| WO2016021973A1 (ko) * | 2014-08-06 | 2016-02-11 | 주식회사 툴젠 | 캄필로박터 제주니 crispr/cas 시스템 유래 rgen을 이용한 유전체 교정 |
| CN107429241B (zh) | 2014-08-14 | 2025-10-24 | 百奥赛图(北京)医药科技股份有限公司 | Dna敲入系统 |
| JP6806668B2 (ja) * | 2014-08-19 | 2021-01-06 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 核酸をプロービングおよびマッピングするためのrna誘導型システム |
| AU2015308910B2 (en) | 2014-08-27 | 2017-12-07 | Caribou Biosciences, Inc. | Methods for increasing Cas9-mediated engineering efficiency |
| US10450584B2 (en) | 2014-08-28 | 2019-10-22 | North Carolina State University | Cas9 proteins and guiding features for DNA targeting and genome editing |
| US10570418B2 (en) | 2014-09-02 | 2020-02-25 | The Regents Of The University Of California | Methods and compositions for RNA-directed target DNA modification |
| CA2956487A1 (en) | 2014-09-12 | 2016-03-17 | E. I. Du Pont De Nemours And Company | Generation of site-specific-integration sites for complex trait loci in corn and soybean, and methods of use |
| EP3197269A1 (en) | 2014-09-26 | 2017-08-02 | Purecircle Usa Inc. | Single nucleotide polymorphism (snp) markers for stevia |
| EP3204496A1 (en) | 2014-10-10 | 2017-08-16 | Editas Medicine, Inc. | Compositions and methods for promoting homology directed repair |
| DK3207124T3 (da) | 2014-10-15 | 2019-08-12 | Regeneron Pharma | Fremgangsmåder og sammensætninger til generering eller bevaring af pluripotente celler |
| US10612042B2 (en) | 2014-10-24 | 2020-04-07 | Avectas Limited | Delivery across cell plasma membranes |
| US9816080B2 (en) | 2014-10-31 | 2017-11-14 | President And Fellows Of Harvard College | Delivery of CAS9 via ARRDC1-mediated microvesicles (ARMMs) |
| US11680268B2 (en) | 2014-11-07 | 2023-06-20 | Editas Medicine, Inc. | Methods for improving CRISPR/Cas-mediated genome-editing |
| US10858662B2 (en) | 2014-11-19 | 2020-12-08 | Institute For Basic Science | Genome editing with split Cas9 expressed from two vectors |
| EP3221457B1 (en) | 2014-11-21 | 2019-03-20 | Regeneron Pharmaceuticals, Inc. | Methods and compositions for targeted genetic modification using paired guide rnas |
| US10900034B2 (en) | 2014-12-03 | 2021-01-26 | Agilent Technologies, Inc. | Guide RNA with chemical modifications |
| CN113699116A (zh) | 2014-12-10 | 2021-11-26 | 明尼苏达大学董事会 | 用于治疗疾病的遗传修饰的细胞、组织和器官 |
| EP3889260A1 (en) | 2014-12-12 | 2021-10-06 | The Broad Institute, Inc. | Protected guide rnas (pgrnas) |
| CA2968336C (en) * | 2014-12-17 | 2021-11-23 | Proqr Therapeutics Ii B.V. | Construct for site directed editing of an adenosine nucleotide in target rna |
| JP6840077B2 (ja) | 2014-12-19 | 2021-03-10 | リジェネロン・ファーマシューティカルズ・インコーポレイテッドRegeneron Pharmaceuticals, Inc. | 単一ステップの複数標的化を通じた標的化された遺伝子修飾のための方法及び組成物 |
| US10196613B2 (en) | 2014-12-19 | 2019-02-05 | Regeneron Pharmaceuticals, Inc. | Stem cells for modeling type 2 diabetes |
| AU2015364286B2 (en) | 2014-12-20 | 2021-11-04 | Arc Bio, Llc | Compositions and methods for targeted depletion, enrichment, and partitioning of nucleic acids using CRISPR/Cas system proteins |
| CA2970370A1 (en) | 2014-12-24 | 2016-06-30 | Massachusetts Institute Of Technology | Crispr having or associated with destabilization domains |
| SG11201704272YA (en) | 2014-12-31 | 2017-06-29 | Synthetic Genomics Inc | Compositions and methods for high efficiency in vivo genome editing |
| WO2016112351A1 (en) | 2015-01-09 | 2016-07-14 | Bio-Rad Laboratories, Inc. | Detection of genome editing |
| CN105821073B (zh) | 2015-01-27 | 2021-08-10 | 中国科学院遗传与发育生物学研究所 | 一种通过基因瞬时表达对整株植物定点改造的方法 |
| LT3250691T (lt) | 2015-01-28 | 2023-09-11 | Caribou Biosciences, Inc. | Crispr hibridiniai dnr/rnr polinukleotidai ir naudojimo būdai |
| WO2016141224A1 (en) | 2015-03-03 | 2016-09-09 | The General Hospital Corporation | Engineered crispr-cas9 nucleases with altered pam specificity |
| KR102194612B1 (ko) | 2015-03-16 | 2020-12-23 | 인스티튜트 오브 제네틱스 앤드 디벨롭멘털 바이오롤지, 차이니즈 아카데미 오브 사이언시스 | 비-유전성 물질을 이용한 식물 게놈의 부위-특이적인 변형 방법 |
| US10450576B2 (en) | 2015-03-27 | 2019-10-22 | E I Du Pont De Nemours And Company | Soybean U6 small nuclear RNA gene promoters and their use in constitutive expression of small RNA genes in plants |
| KR102888521B1 (ko) | 2015-04-06 | 2025-11-19 | 더 보드 어브 트러스티스 어브 더 리랜드 스탠포드 주니어 유니버시티 | Crispr/cas-매개 유전자 조절을 위한 화학적으로 변형된 가이드 rna |
| GB201506509D0 (en) | 2015-04-16 | 2015-06-03 | Univ Wageningen | Nuclease-mediated genome editing |
| EP4019975A1 (en) | 2015-04-24 | 2022-06-29 | Editas Medicine, Inc. | Evaluation of cas9 molecule/guide rna molecule complexes |
| US11845928B2 (en) | 2015-05-04 | 2023-12-19 | Tsinghua University | Methods and kits for fragmenting DNA |
| PL3291679T3 (pl) | 2015-05-06 | 2022-04-25 | Snipr Technologies Limited | Zmiana populacji drobnoustrojowych i modyfikowanie mikrobioty |
| CA2986310A1 (en) | 2015-05-11 | 2016-11-17 | Editas Medicine, Inc. | Optimized crispr/cas9 systems and methods for gene editing in stem cells |
| BR112017024534A2 (pt) * | 2015-05-15 | 2018-07-24 | Pioneer Hi Bred Int | sistemas de rna-guia/endonuclease cas9 inovadores |
| EP3294880A4 (en) * | 2015-05-15 | 2018-12-26 | Dharmacon, Inc. | Synthetic single guide rna for cas9-mediated gene editing |
| EP3095870A1 (en) | 2015-05-19 | 2016-11-23 | Kws Saat Se | Methods for the in planta transformation of plants and manufacturing processes and products based and obtainable therefrom |
| AU2016270649B2 (en) | 2015-05-29 | 2022-04-21 | North Carolina State University | Methods for screening bacteria, archaea, algae, and yeast using crispr nucleic acids |
| US20160346360A1 (en) | 2015-05-29 | 2016-12-01 | Agenovir Corporation | Compositions and methods for cell targeted hpv treatment |
| US10117911B2 (en) | 2015-05-29 | 2018-11-06 | Agenovir Corporation | Compositions and methods to treat herpes simplex virus infections |
| EP3302053B1 (en) | 2015-06-02 | 2021-03-17 | Monsanto Technology LLC | Compositions and methods for delivery of a polynucleotide into a plant |
| CN108368502B (zh) | 2015-06-03 | 2022-03-18 | 内布拉斯加大学董事委员会 | 使用单链dna的dna编辑 |
| WO2016196655A1 (en) | 2015-06-03 | 2016-12-08 | The Regents Of The University Of California | Cas9 variants and methods of use thereof |
| WO2016201047A1 (en) | 2015-06-09 | 2016-12-15 | Editas Medicine, Inc. | Crispr/cas-related methods and compositions for improving transplantation |
| KR20180034389A (ko) | 2015-06-12 | 2018-04-04 | 론자 워커스빌 아이엔씨. | 합성 전사인자를 이용한 핵 역분화 방법 |
| ES2960226T3 (es) * | 2015-06-15 | 2024-03-01 | Univ North Carolina State | Métodos y composiciones para la administración eficiente de ácidos nucleicos y antimicrobianos basados en ARN |
| US9790490B2 (en) * | 2015-06-18 | 2017-10-17 | The Broad Institute Inc. | CRISPR enzymes and systems |
| WO2016205759A1 (en) | 2015-06-18 | 2016-12-22 | The Broad Institute Inc. | Engineering and optimization of systems, methods, enzymes and guide scaffolds of cas9 orthologs and variants for sequence manipulation |
| MX392008B (es) * | 2015-06-18 | 2025-03-21 | Broad Inst Inc | Mutaciones de la enzima crispr que reducen los efectos fuera del blanco |
| US11414657B2 (en) | 2015-06-29 | 2022-08-16 | Ionis Pharmaceuticals, Inc. | Modified CRISPR RNA and modified single CRISPR RNA and uses thereof |
| IL295616A (en) | 2015-07-31 | 2022-10-01 | Us Health | Adapted cells and treatment methods |
| US9580727B1 (en) | 2015-08-07 | 2017-02-28 | Caribou Biosciences, Inc. | Compositions and methods of engineered CRISPR-Cas9 systems using split-nexus Cas9-associated polynucleotides |
| EP3334832A4 (en) | 2015-08-14 | 2019-06-26 | Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences | PROCESS FOR THE PREPARATION OF GLYPHOSATE-RESISTANT RICE BY TARGETED NUCLEOTIDE SUBSTITUTION |
| EP3985111A1 (en) | 2015-08-19 | 2022-04-20 | Arc Bio, LLC | Capture of nucleic acids using a nucleic acid-guided nuclease-based system |
| US9512446B1 (en) | 2015-08-28 | 2016-12-06 | The General Hospital Corporation | Engineered CRISPR-Cas9 nucleases |
| US9926546B2 (en) | 2015-08-28 | 2018-03-27 | The General Hospital Corporation | Engineered CRISPR-Cas9 nucleases |
| WO2017040348A1 (en) | 2015-08-28 | 2017-03-09 | The General Hospital Corporation | Engineered crispr-cas9 nucleases |
| WO2017044419A1 (en) | 2015-09-08 | 2017-03-16 | University Of Massachusetts | Dnase h activity of neisseria meningitidis cas9 |
| WO2017044843A1 (en) | 2015-09-11 | 2017-03-16 | The General Hospital Corporation | Full interrogation of nuclease dsbs and sequencing (find-seq) |
| EP3786294A1 (en) | 2015-09-24 | 2021-03-03 | Editas Medicine, Inc. | Use of exonucleases to improve crispr/cas-mediated genome editing |
| WO2017058751A1 (en) | 2015-09-28 | 2017-04-06 | North Carolina State University | Methods and compositions for sequence specific antimicrobials |
| US9850484B2 (en) | 2015-09-30 | 2017-12-26 | The General Hospital Corporation | Comprehensive in vitro reporting of cleavage events by sequencing (Circle-seq) |
| CN108367081A (zh) | 2015-10-14 | 2018-08-03 | 生命技术公司 | 核糖核蛋白转染剂 |
| WO2017070605A1 (en) * | 2015-10-22 | 2017-04-27 | The Broad Institute Inc. | Type vi-b crispr enzymes and systems |
| WO2017070598A1 (en) | 2015-10-23 | 2017-04-27 | Caribou Biosciences, Inc. | Engineered crispr class 2 cross-type nucleic-acid targeting nucleic acids |
| EP4434589A3 (en) | 2015-10-23 | 2025-05-14 | President and Fellows of Harvard College | Evolved cas9 proteins for gene editing |
| EP3371329B1 (en) | 2015-11-03 | 2026-03-25 | President and Fellows of Harvard College | Method and apparatus for volumetric imaging of a three-dimensional nucleic acid containing matrix |
| WO2017079428A1 (en) | 2015-11-04 | 2017-05-11 | President And Fellows Of Harvard College | Site specific germline modification |
| WO2017081288A1 (en) | 2015-11-11 | 2017-05-18 | Lonza Ltd | Crispr-associated (cas) proteins with reduced immunogenicity |
| KR101906491B1 (ko) * | 2015-11-30 | 2018-12-05 | 기초과학연구원 | F. novicida 유래 Cas9을 포함하는 유전체 교정용 조성물 |
| RU2018117360A (ru) | 2015-12-04 | 2020-01-09 | Карибо Биосайенсиз, Инк. | Сконструированные нуклеиновые кислоты, нацеленные на нуклеиновую кислоту |
| KR20180084756A (ko) | 2015-12-07 | 2018-07-25 | 지머젠 인코포레이티드 | 코리네박테리움 글루타미컴으로부터의 프로모터 |
| US11208649B2 (en) | 2015-12-07 | 2021-12-28 | Zymergen Inc. | HTP genomic engineering platform |
| US9988624B2 (en) | 2015-12-07 | 2018-06-05 | Zymergen Inc. | Microbial strain improvement by a HTP genomic engineering platform |
| WO2017112620A1 (en) | 2015-12-22 | 2017-06-29 | North Carolina State University | Methods and compositions for delivery of crispr based antimicrobials |
| EP3397760A2 (en) | 2015-12-30 | 2018-11-07 | Avectas Limited | Vector-free delivery of gene editing proteins and compositions to cells and tissues |
| JP7012645B2 (ja) | 2016-01-11 | 2022-01-28 | ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー | キメラタンパク質および免疫治療の方法 |
| MX2018008344A (es) | 2016-01-11 | 2018-12-06 | Univ Leland Stanford Junior | Proteinas quimericas y metodos para regular la expresion genica. |
| AR107469A1 (es) | 2016-01-27 | 2018-05-02 | Oncorus Inc | Vectores virales oncolíticos y sus usos |
| WO2017142999A2 (en) * | 2016-02-18 | 2017-08-24 | President And Fellows Of Harvard College | Methods and systems of molecular recording by crispr-cas system |
| WO2017147278A1 (en) | 2016-02-25 | 2017-08-31 | The Children's Medical Center Corporation | Customized class switch of immunoglobulin genes in lymphoma and hybridoma by crispr/cas9 technology |
| EP3423083A4 (en) * | 2016-03-04 | 2019-10-02 | Indoor Biotechnologies Inc. | FEL-D-1 KNOCKOUTS AND ASSOCIATED COMPOSITIONS AND METHODS BASED ON CRISPR-CAS9 GENERALIZATION |
| US11597924B2 (en) | 2016-03-25 | 2023-03-07 | Editas Medicine, Inc. | Genome editing systems comprising repair-modulating enzyme molecules and methods of their use |
| WO2017165862A1 (en) | 2016-03-25 | 2017-09-28 | Editas Medicine, Inc. | Systems and methods for treating alpha 1-antitrypsin (a1at) deficiency |
| WO2017180694A1 (en) | 2016-04-13 | 2017-10-19 | Editas Medicine, Inc. | Cas9 fusion molecules gene editing systems, and methods of use thereof |
| CA3210120C (en) | 2016-04-25 | 2024-04-09 | President And Fellows Of Harvard College | Hybridization chain reaction methods for in situ molecular detection |
| CN105907785B (zh) * | 2016-05-05 | 2020-02-07 | 苏州吉玛基因股份有限公司 | 化学合成的crRNA用于CRISPR/Cpf1系统在基因编辑中的应用 |
| US11293033B2 (en) | 2016-05-18 | 2022-04-05 | Amyris, Inc. | Compositions and methods for genomic integration of nucleic acids into exogenous landing pads |
| CN109475109B (zh) | 2016-05-20 | 2021-10-29 | 瑞泽恩制药公司 | 用于使用多个引导rna来破坏免疫耐受性的方法 |
| KR102811233B1 (ko) | 2016-06-02 | 2025-05-29 | 시그마-알드리치 컴퍼니., 엘엘씨 | 프로그램가능한 dna 결합 단백질을 사용한, 표적화된 게놈 변형의 개선 |
| GB201609811D0 (en) | 2016-06-05 | 2016-07-20 | Snipr Technologies Ltd | Methods, cells, systems, arrays, RNA and kits |
| US10767175B2 (en) | 2016-06-08 | 2020-09-08 | Agilent Technologies, Inc. | High specificity genome editing using chemically modified guide RNAs |
| US11293021B1 (en) | 2016-06-23 | 2022-04-05 | Inscripta, Inc. | Automated cell processing methods, modules, instruments, and systems |
| CN109688820B (zh) | 2016-06-24 | 2023-01-10 | 科罗拉多州立大学董事会(法人团体) | 用于生成条形码化组合文库的方法 |
| EP3474849B1 (en) | 2016-06-27 | 2025-05-21 | The Broad Institute, Inc. | Compositions and methods for detecting and treating diabetes |
| KR102345899B1 (ko) | 2016-06-30 | 2021-12-31 | 지머젠 인코포레이티드 | 박테리아 헤모글로빈 라이브러리를 생성하는 방법 및 이의 용도 |
| JP2019519241A (ja) | 2016-06-30 | 2019-07-11 | ザイマージェン インコーポレイテッド | グルコース透過酵素ライブラリーを生成するための方法およびその使用 |
| RU2721125C1 (ru) | 2016-07-29 | 2020-05-18 | Регенерон Фармасьютикалз, Инк. | Мыши, содержащие мутации, вследствие которых экспрессируется укороченный на с-конце фибриллин-1 |
| CA3032822A1 (en) | 2016-08-02 | 2018-02-08 | Editas Medicine, Inc. | Compositions and methods for treating cep290 associated disease |
| US11078481B1 (en) | 2016-08-03 | 2021-08-03 | KSQ Therapeutics, Inc. | Methods for screening for cancer targets |
| JP7231935B2 (ja) | 2016-08-03 | 2023-03-08 | プレジデント アンド フェローズ オブ ハーバード カレッジ | アデノシン核酸塩基編集因子およびそれらの使用 |
| US11661590B2 (en) | 2016-08-09 | 2023-05-30 | President And Fellows Of Harvard College | Programmable CAS9-recombinase fusion proteins and uses thereof |
| WO2018039438A1 (en) | 2016-08-24 | 2018-03-01 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| GB2569252A (en) | 2016-08-31 | 2019-06-12 | Harvard College | Methods of combining the detection of biomolecules into a single assay using fluorescent in situ sequencing |
| US11078483B1 (en) | 2016-09-02 | 2021-08-03 | KSQ Therapeutics, Inc. | Methods for measuring and improving CRISPR reagent function |
| US20190225974A1 (en) | 2016-09-23 | 2019-07-25 | BASF Agricultural Solutions Seed US LLC | Targeted genome optimization in plants |
| EP3518981A4 (en) | 2016-10-03 | 2020-06-10 | President and Fellows of Harvard College | THERAPEUTIC RNA DELIVERY THROUGH ARRDC1 MICROVESICLES |
| AU2017342543B2 (en) | 2016-10-14 | 2024-06-27 | President And Fellows Of Harvard College | AAV delivery of nucleobase editors |
| CN110290813A (zh) | 2016-10-14 | 2019-09-27 | 通用医疗公司 | 表观遗传学调控的位点特异性核酸酶 |
| GB201617559D0 (en) | 2016-10-17 | 2016-11-30 | University Court Of The University Of Edinburgh The | Swine comprising modified cd163 and associated methods |
| EP4338799A3 (en) | 2016-10-18 | 2024-06-05 | Regents of the University of Minnesota | Tumor infiltrating lymphocytes and methods of therapy |
| WO2018144097A1 (en) | 2016-11-04 | 2018-08-09 | Akeagen Llc | Genetically modified non-human animals and methods for producing heavy chain-only antibodies |
| US9816093B1 (en) | 2016-12-06 | 2017-11-14 | Caribou Biosciences, Inc. | Engineered nucleic acid-targeting nucleic acids |
| WO2018119010A1 (en) | 2016-12-19 | 2018-06-28 | Editas Medicine, Inc. | Assessing nuclease cleavage |
| WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
| WO2018129368A2 (en) | 2017-01-06 | 2018-07-12 | Editas Medicine, Inc. | Methods of assessing nuclease cleavage |
| JP7215716B2 (ja) | 2017-01-13 | 2023-01-31 | 学校法人自治医科大学 | 肝臓ゲノム上の凝固関連因子遺伝子を破壊するためのaavベクター |
| CN108314736B (zh) * | 2017-01-18 | 2021-08-31 | 李燕强 | 一种促进rna降解的方法 |
| WO2018136758A1 (en) | 2017-01-23 | 2018-07-26 | Regeneron Pharmaceuticals, Inc. | Hsd17b13 variants and uses thereof |
| CN110662556A (zh) | 2017-03-09 | 2020-01-07 | 哈佛大学的校长及成员们 | 癌症疫苗 |
| US11898179B2 (en) | 2017-03-09 | 2024-02-13 | President And Fellows Of Harvard College | Suppression of pain by gene editing |
| JP2020510439A (ja) | 2017-03-10 | 2020-04-09 | プレジデント アンド フェローズ オブ ハーバード カレッジ | シトシンからグアニンへの塩基編集因子 |
| WO2018170184A1 (en) | 2017-03-14 | 2018-09-20 | Editas Medicine, Inc. | Systems and methods for the treatment of hemoglobinopathies |
| WO2018176009A1 (en) | 2017-03-23 | 2018-09-27 | President And Fellows Of Harvard College | Nucleobase editors comprising nucleic acid programmable dna binding proteins |
| US11913015B2 (en) | 2017-04-17 | 2024-02-27 | University Of Maryland, College Park | Embryonic cell cultures and methods of using the same |
| US12058986B2 (en) | 2017-04-20 | 2024-08-13 | Egenesis, Inc. | Method for generating a genetically modified pig with inactivated porcine endogenous retrovirus (PERV) elements |
| BR112019021719A2 (pt) | 2017-04-21 | 2020-06-16 | The General Hospital Corporation | Variantes de cpf1 (cas12a) com especificidade para pam alterada |
| WO2018197520A1 (en) | 2017-04-24 | 2018-11-01 | Dupont Nutrition Biosciences Aps | Methods and compositions of anti-crispr proteins for use in plants |
| EP3615672A1 (en) | 2017-04-28 | 2020-03-04 | Editas Medicine, Inc. | Methods and systems for analyzing guide rna molecules |
| WO2018209158A2 (en) | 2017-05-10 | 2018-11-15 | Editas Medicine, Inc. | Crispr/rna-guided nuclease systems and methods |
| US11560566B2 (en) | 2017-05-12 | 2023-01-24 | President And Fellows Of Harvard College | Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation |
| CN110662835B (zh) * | 2017-05-19 | 2023-04-28 | 清华大学 | 工程化改造用于由增强的指导RNA优化的基因编辑和转录调节的最小化SaCas9 CRISPR/Cas系统 |
| EP3630970A4 (en) | 2017-05-25 | 2020-12-30 | The General Hospital Corporation | BASIC EDITORS WITH IMPROVED PRECISION AND SPECIFICITY |
| JP6628385B6 (ja) | 2017-05-31 | 2020-03-04 | 国立大学法人 東京大学 | 改変されたCas9タンパク質及びその用途 |
| EP3878961A1 (en) | 2017-06-06 | 2021-09-15 | Zymergen, Inc. | A htp genomic engineering platform for improving escherichia coli |
| WO2018226900A2 (en) | 2017-06-06 | 2018-12-13 | Zymergen Inc. | A htp genomic engineering platform for improving fungal strains |
| EP3635104A1 (en) | 2017-06-09 | 2020-04-15 | Editas Medicine, Inc. | Engineered cas9 nucleases |
| CN109963944A (zh) | 2017-06-20 | 2019-07-02 | 江苏恒瑞医药股份有限公司 | 体外敲除T细胞中靶基因的方法以及该方法中使用的crRNA |
| US10011849B1 (en) | 2017-06-23 | 2018-07-03 | Inscripta, Inc. | Nucleic acid-guided nucleases |
| US9982279B1 (en) | 2017-06-23 | 2018-05-29 | Inscripta, Inc. | Nucleic acid-guided nucleases |
| AU2018291041B2 (en) | 2017-06-30 | 2021-08-05 | Inscripta, Inc. | Automated cell processing methods, modules, instruments, and systems |
| EP3645021A4 (en) | 2017-06-30 | 2021-04-21 | Intima Bioscience, Inc. | ADENO-ASSOCIATED VIRAL VECTORS FOR GENE THERAPY |
| WO2019014564A1 (en) | 2017-07-14 | 2019-01-17 | Editas Medicine, Inc. | SYSTEMS AND METHODS OF TARGETED INTEGRATION AND GENOME EDITING AND DETECTION THEREOF WITH INTEGRATED PRIMING SITES |
| EP3658165A4 (en) | 2017-07-26 | 2021-09-01 | Oncorus, Inc. | ONCOLYTIC VIRAL VECTORS AND THEIR USES |
| US11732274B2 (en) | 2017-07-28 | 2023-08-22 | President And Fellows Of Harvard College | Methods and compositions for evolving base editors using phage-assisted continuous evolution (PACE) |
| EP3585160A2 (en) | 2017-07-31 | 2020-01-01 | Regeneron Pharmaceuticals, Inc. | Crispr reporter non-human animals and uses thereof |
| SG11201912235PA (en) | 2017-07-31 | 2020-01-30 | Regeneron Pharma | Cas-transgenic mouse embryonic stem cells and mice and uses thereof |
| AU2018309714A1 (en) | 2017-07-31 | 2020-01-30 | Regeneron Pharmaceuticals, Inc. | Assessment of CRISPR/Cas-induced recombination with an exogenous donor nucleic acid in vivo |
| EP3672612A4 (en) | 2017-08-23 | 2021-09-29 | The General Hospital Corporation | GENETICALLY MODIFIED CRISPR-CAS9 NUCLEASES WITH MODIFIED PAM SPECIFICITY |
| US10738327B2 (en) | 2017-08-28 | 2020-08-11 | Inscripta, Inc. | Electroporation cuvettes for automation |
| WO2019139645A2 (en) | 2017-08-30 | 2019-07-18 | President And Fellows Of Harvard College | High efficiency base editors comprising gam |
| WO2019049913A1 (ja) | 2017-09-05 | 2019-03-14 | 国立大学法人 東京大学 | 改変されたCas9タンパク質及びその用途 |
| ES2962277T3 (es) | 2017-09-29 | 2024-03-18 | Regeneron Pharma | Roedores que comprenden un locus Ttr humanizado y métodos de uso |
| US10435713B2 (en) | 2017-09-30 | 2019-10-08 | Inscripta, Inc. | Flow through electroporation instrumentation |
| US11725228B2 (en) | 2017-10-11 | 2023-08-15 | The General Hospital Corporation | Methods for detecting site-specific and spurious genomic deamination induced by base editing technologies |
| KR20200121782A (ko) | 2017-10-16 | 2020-10-26 | 더 브로드 인스티튜트, 인코퍼레이티드 | 아데노신 염기 편집제의 용도 |
| JP7423520B2 (ja) | 2017-11-16 | 2024-01-29 | アストラゼネカ・アクチエボラーグ | Cas9ベースノックイン方針の効力を改善するための組成物及び方法 |
| US12406749B2 (en) | 2017-12-15 | 2025-09-02 | The Broad Institute, Inc. | Systems and methods for predicting repair outcomes in genetic engineering |
| US20200362366A1 (en) | 2018-01-12 | 2020-11-19 | Basf Se | Gene underlying the number of spikelets per spike qtl in wheat on chromosome 7a |
| WO2019165168A1 (en) | 2018-02-23 | 2019-08-29 | Pioneer Hi-Bred International, Inc. | Novel cas9 orthologs |
| EP3765614A1 (en) | 2018-03-14 | 2021-01-20 | Editas Medicine, Inc. | Systems and methods for the treatment of hemoglobinopathies |
| KR20240038811A (ko) | 2018-03-19 | 2024-03-25 | 리제너론 파마슈티칼스 인코포레이티드 | CRISPR/Cas 시스템을 사용한 동물에서의 전사 조절 |
| US10760075B2 (en) | 2018-04-30 | 2020-09-01 | Snipr Biome Aps | Treating and preventing microbial infections |
| US10435662B1 (en) | 2018-03-29 | 2019-10-08 | Inscripta, Inc. | Automated control of cell growth rates for induction and transformation |
| US20210155959A1 (en) | 2018-04-06 | 2021-05-27 | Children's Medical Center Corporation | Compositions and methods for somatic cell reprogramming and modulating imprinting |
| WO2019200004A1 (en) | 2018-04-13 | 2019-10-17 | Inscripta, Inc. | Automated cell processing instruments comprising reagent cartridges |
| WO2019204378A1 (en) | 2018-04-17 | 2019-10-24 | The General Hospital Corporation | Sensitive in vitro assays for substrate preferences and sites of nucleic acid binding, modifying, and cleaving agents |
| US10557216B2 (en) | 2018-04-24 | 2020-02-11 | Inscripta, Inc. | Automated instrumentation for production of T-cell receptor peptide libraries |
| US10858761B2 (en) | 2018-04-24 | 2020-12-08 | Inscripta, Inc. | Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells |
| US10501738B2 (en) | 2018-04-24 | 2019-12-10 | Inscripta, Inc. | Automated instrumentation for production of peptide libraries |
| CN112654710A (zh) | 2018-05-16 | 2021-04-13 | 辛瑟高公司 | 用于指导rna设计和使用的方法和系统 |
| US12157760B2 (en) | 2018-05-23 | 2024-12-03 | The Broad Institute, Inc. | Base editors and uses thereof |
| AU2019291918B2 (en) | 2018-06-29 | 2025-06-12 | Editas Medicine, Inc. | Synthetic guide molecules, compositions and methods relating thereto |
| WO2020005383A1 (en) | 2018-06-30 | 2020-01-02 | Inscripta, Inc. | Instruments, modules, and methods for improved detection of edited sequences in live cells |
| US12522807B2 (en) | 2018-07-09 | 2026-01-13 | The Broad Institute, Inc. | RNA programmable epigenetic RNA modifiers and uses thereof |
| US11142740B2 (en) | 2018-08-14 | 2021-10-12 | Inscripta, Inc. | Detection of nuclease edited sequences in automated modules and instruments |
| US10532324B1 (en) | 2018-08-14 | 2020-01-14 | Inscripta, Inc. | Instruments, modules, and methods for improved detection of edited sequences in live cells |
| US10752874B2 (en) | 2018-08-14 | 2020-08-25 | Inscripta, Inc. | Instruments, modules, and methods for improved detection of edited sequences in live cells |
| CN113195715A (zh) | 2018-08-29 | 2021-07-30 | 阿迈瑞斯公司 | 基于选择的测定法的细胞和方法 |
| CN112955540A (zh) | 2018-08-30 | 2021-06-11 | 因思科瑞普特公司 | 在自动化模块和仪器中对经核酸酶经编辑的序列的改进的检测 |
| EP3847251A1 (en) | 2018-09-07 | 2021-07-14 | Astrazeneca AB | Compositions and methods for improved nucleases |
| US12264313B2 (en) | 2018-10-01 | 2025-04-01 | North Carolina State University | Recombinant type I CRISPR-Cas system and uses thereof for genome modification and alteration of expression |
| WO2020072253A1 (en) | 2018-10-01 | 2020-04-09 | North Carolina State University | Recombinant type i crispr-cas system and uses thereof for screening for variant cells |
| US12264330B2 (en) | 2018-10-01 | 2025-04-01 | North Carolina State University | Recombinant type I CRISPR-Cas system and uses thereof for killing target cells |
| WO2020072248A1 (en) | 2018-10-01 | 2020-04-09 | North Carolina State University | Recombinant type i crispr-cas system |
| WO2020076976A1 (en) | 2018-10-10 | 2020-04-16 | Readcoor, Inc. | Three-dimensional spatial molecular indexing |
| US11851663B2 (en) | 2018-10-14 | 2023-12-26 | Snipr Biome Aps | Single-vector type I vectors |
| EP3870697A4 (en) | 2018-10-22 | 2022-11-09 | Inscripta, Inc. | GMO ENZYMES |
| US11214781B2 (en) | 2018-10-22 | 2022-01-04 | Inscripta, Inc. | Engineered enzyme |
| WO2020092453A1 (en) | 2018-10-29 | 2020-05-07 | The Broad Institute, Inc. | Nucleobase editors comprising geocas9 and uses thereof |
| JP2022513408A (ja) | 2018-10-31 | 2022-02-07 | ザイマージェン インコーポレイテッド | Dnaライブラリーの多重化決定論的アセンブリー |
| JP2022505440A (ja) | 2018-11-01 | 2022-01-14 | キージーン ナムローゼ フェンノートシャップ | 植物細胞におけるCRISPR/Casゲノム編集のためのデュアルガイドRNA |
| JP7530355B2 (ja) | 2018-11-28 | 2024-08-07 | キージーン ナムローゼ フェンノートシャップ | エンドヌクレアーゼ保護による標的化濃縮 |
| KR20200071198A (ko) | 2018-12-10 | 2020-06-19 | 네오이뮨텍, 인코퍼레이티드 | Nrf2 발현 조절 기반 T 세포 항암면역치료법 |
| US12365888B2 (en) | 2018-12-14 | 2025-07-22 | Pioneer Hi-Bred International, Inc. | CRISPR-Cas systems for genome editing |
| US11690362B2 (en) | 2018-12-20 | 2023-07-04 | Regeneran Pharmaceuticals, Inc. | Nuclease-mediated repeat expansion |
| WO2020154500A1 (en) | 2019-01-23 | 2020-07-30 | The Broad Institute, Inc. | Supernegatively charged proteins and uses thereof |
| WO2020163396A1 (en) | 2019-02-04 | 2020-08-13 | The General Hospital Corporation | Adenine dna base editor variants with reduced off-target rna editing |
| WO2020168234A1 (en) * | 2019-02-14 | 2020-08-20 | Metagenomi Ip Technologies, Llc | Enzymes with ruvc domains |
| US10913941B2 (en) | 2019-02-14 | 2021-02-09 | Metagenomi Ip Technologies, Llc | Enzymes with RuvC domains |
| US11053515B2 (en) | 2019-03-08 | 2021-07-06 | Zymergen Inc. | Pooled genome editing in microbes |
| WO2020185590A1 (en) | 2019-03-08 | 2020-09-17 | Zymergen Inc. | Iterative genome editing in microbes |
| SG11202108090XA (en) | 2019-03-18 | 2021-08-30 | Regeneron Pharma | Crispr/cas dropout screening platform to reveal genetic vulnerabilities associated with tau aggregation |
| WO2020190932A1 (en) | 2019-03-18 | 2020-09-24 | Regeneron Pharmaceuticals, Inc. | Crispr/cas screening platform to identify genetic modifiers of tau seeding or aggregation |
| MX2021011426A (es) | 2019-03-19 | 2022-03-11 | Broad Inst Inc | Metodos y composiciones para editar secuencias de nucleótidos. |
| AU2020247900A1 (en) | 2019-03-25 | 2021-11-04 | Inscripta, Inc. | Simultaneous multiplex genome editing in yeast |
| US11001831B2 (en) | 2019-03-25 | 2021-05-11 | Inscripta, Inc. | Simultaneous multiplex genome editing in yeast |
| JP7743308B2 (ja) | 2019-03-27 | 2025-09-24 | パイオニア ハイ-ブレッド インターナショナル, インコーポレイテッド | 植物外稙片の形質転換 |
| AU2020256225B9 (en) | 2019-04-03 | 2025-04-10 | Regeneron Pharmaceuticals, Inc. | Methods and compositions for insertion of antibody coding sequences into a safe harbor locus |
| US11111504B2 (en) | 2019-04-04 | 2021-09-07 | Regeneron Pharmaceuticals, Inc. | Methods for scarless introduction of targeted modifications into targeting vectors |
| WO2020206139A1 (en) | 2019-04-04 | 2020-10-08 | Regeneron Pharmaceuticals, Inc. | Non-human animals comprising a humanized coagulation factor 12 locus |
| US20220186263A1 (en) | 2019-04-05 | 2022-06-16 | Osaka University | Method for producing knock-in cell |
| US20220162648A1 (en) | 2019-04-12 | 2022-05-26 | Astrazeneca Ab | Compositions and methods for improved gene editing |
| WO2020214842A1 (en) | 2019-04-17 | 2020-10-22 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| EP3801011A1 (en) | 2019-06-04 | 2021-04-14 | Regeneron Pharmaceuticals, Inc. | Non-human animals comprising a humanized ttr locus with a beta-slip mutation and methods of use |
| WO2020247587A1 (en) | 2019-06-06 | 2020-12-10 | Inscripta, Inc. | Curing for recursive nucleic acid-guided cell editing |
| AU2020289581B2 (en) | 2019-06-07 | 2024-11-21 | Regeneron Pharmaceuticals, Inc. | Non-human animals comprising a humanized albumin locus |
| CA3137765A1 (en) | 2019-06-14 | 2020-12-17 | Regeneron Pharmaceuticals, Inc. | Models of tauopathy |
| US10907125B2 (en) | 2019-06-20 | 2021-02-02 | Inscripta, Inc. | Flow through electroporation modules and instrumentation |
| EP3986909A4 (en) | 2019-06-21 | 2023-08-02 | Inscripta, Inc. | Genome-wide rationally-designed mutations leading to enhanced lysine production in e. coli |
| US10927385B2 (en) | 2019-06-25 | 2021-02-23 | Inscripta, Inc. | Increased nucleic-acid guided cell editing in yeast |
| CN114245803A (zh) | 2019-08-06 | 2022-03-25 | 儿童医疗中心有限公司 | 用于重构小神经胶质细胞的方法和组合物 |
| CN214973877U (zh) | 2019-08-09 | 2021-12-03 | 胡桃钳医疗公司 | 用于形成治疗性多核苷酸的微流体设备及微流体路径装置 |
| CA3153980A1 (en) | 2019-09-13 | 2021-03-18 | Regeneron Pharmaceuticals, Inc. | Transcription modulation in animals using crispr/cas systems delivered by lipid nanoparticles |
| EP4034088A4 (en) | 2019-09-26 | 2023-10-11 | President and Fellows of Harvard College | Minimal arrestin domain containing protein 1 (arrdc1) constructs |
| US12435330B2 (en) | 2019-10-10 | 2025-10-07 | The Broad Institute, Inc. | Methods and compositions for prime editing RNA |
| AU2020379046B2 (en) | 2019-11-08 | 2025-03-13 | Regeneron Pharmaceuticals, Inc. | CRISPR and AAV strategies for X-linked juvenile retinoschisis therapy |
| US11203762B2 (en) | 2019-11-19 | 2021-12-21 | Inscripta, Inc. | Methods for increasing observed editing in bacteria |
| WO2021108363A1 (en) | 2019-11-25 | 2021-06-03 | Regeneron Pharmaceuticals, Inc. | Crispr/cas-mediated upregulation of humanized ttr allele |
| CN114787347B (zh) | 2019-12-10 | 2024-07-12 | 因思科瑞普特公司 | 新颖的mad核酸酶 |
| US10704033B1 (en) | 2019-12-13 | 2020-07-07 | Inscripta, Inc. | Nucleic acid-guided nucleases |
| CA3157127A1 (en) | 2019-12-18 | 2021-06-24 | Aamir MIR | Cascade/dcas3 complementation assays for in vivo detection of nucleic acid-guided nuclease edited cells |
| US10689669B1 (en) | 2020-01-11 | 2020-06-23 | Inscripta, Inc. | Automated multi-module cell processing methods, instruments, and systems |
| WO2021151085A2 (en) | 2020-01-24 | 2021-07-29 | The General Hospital Corporation | Crispr-cas enzymes with enhanced on-target activity |
| US12312613B2 (en) | 2020-01-24 | 2025-05-27 | The General Hospital Corporation | Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants |
| US11225674B2 (en) | 2020-01-27 | 2022-01-18 | Inscripta, Inc. | Electroporation modules and instrumentation |
| WO2021155063A1 (en) | 2020-01-29 | 2021-08-05 | Readcoor, Llc | Compositions and methods for analyte detection |
| JP2023515671A (ja) | 2020-03-04 | 2023-04-13 | リジェネロン・ファーマシューティカルズ・インコーポレイテッド | 免疫療法に対する腫瘍細胞の感作のための方法及び組成物 |
| EP4119166A4 (en) | 2020-03-12 | 2024-06-05 | Institute for Basic Science | Composition for inducing apoptosis of cells having genomic sequence variation and method for inducing apoptosis of cells by using composition |
| WO2021195079A1 (en) | 2020-03-23 | 2021-09-30 | Regeneron Pharmaceuticals, Inc. | Non-human animals comprising a humanized ttr locus comprising a v30m mutation and methods of use |
| ES3055163T3 (en) * | 2020-03-31 | 2026-02-10 | Metagenomi Inc | Class ii, type ii crispr systems |
| WO2021202938A1 (en) | 2020-04-03 | 2021-10-07 | Creyon Bio, Inc. | Oligonucleotide-based machine learning |
| US20210332388A1 (en) | 2020-04-24 | 2021-10-28 | Inscripta, Inc. | Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells |
| WO2021224633A1 (en) | 2020-05-06 | 2021-11-11 | Orchard Therapeutics (Europe) Limited | Treatment for neurodegenerative diseases |
| WO2021226558A1 (en) | 2020-05-08 | 2021-11-11 | The Broad Institute, Inc. | Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence |
| US11787841B2 (en) | 2020-05-19 | 2023-10-17 | Inscripta, Inc. | Rationally-designed mutations to the thrA gene for enhanced lysine production in E. coli |
| US20220049303A1 (en) | 2020-08-17 | 2022-02-17 | Readcoor, Llc | Methods and systems for spatial mapping of genetic variants |
| EP4214314A4 (en) | 2020-09-15 | 2024-10-16 | Inscripta, Inc. | Crispr editing to embed nucleic acid landing pads into genomes of live cells |
| US20230407366A1 (en) | 2020-10-06 | 2023-12-21 | Keygene N.V. | Targeted sequence addition |
| US20230416709A1 (en) | 2020-11-06 | 2023-12-28 | Editforce, Inc. | Foki nuclease domain mutant |
| US11512297B2 (en) | 2020-11-09 | 2022-11-29 | Inscripta, Inc. | Affinity tag for recombination protein recruitment |
| EP4244347A1 (en) | 2020-11-10 | 2023-09-20 | Industrial Microbes, Inc. | Microorganisms capable of producing poly(hiba) from feedstock |
| WO2022112316A1 (en) | 2020-11-24 | 2022-06-02 | Keygene N.V. | Targeted enrichment using nanopore selective sequencing |
| WO2022120022A1 (en) | 2020-12-02 | 2022-06-09 | Regeneron Pharmaceuticals, Inc. | Crispr sam biosensor cell lines and methods of use thereof |
| WO2022146497A1 (en) | 2021-01-04 | 2022-07-07 | Inscripta, Inc. | Mad nucleases |
| US20240376451A1 (en) | 2021-01-07 | 2024-11-14 | Inscripta, Inc. | Mad nucleases |
| US11884924B2 (en) | 2021-02-16 | 2024-01-30 | Inscripta, Inc. | Dual strand nucleic acid-guided nickase editing |
| EP4323529A1 (en) | 2021-04-15 | 2024-02-21 | Keygene N.V. | Mobile endonucleases for heritable mutations |
| IL305936A (en) | 2021-04-15 | 2023-11-01 | Keygene Nv | Integrated regeneration of recalcitrant plants |
| EP4337769A1 (en) | 2021-05-10 | 2024-03-20 | SQZ Biotechnologies Company | Methods for delivering genome editing molecules to the nucleus or cytosol of a cell and uses thereof |
| JP2024518793A (ja) | 2021-05-27 | 2024-05-02 | アストラゼネカ・アクチエボラーグ | 向上した安定性を有するcas9エフェクタータンパク質 |
| WO2022251644A1 (en) | 2021-05-28 | 2022-12-01 | Lyell Immunopharma, Inc. | Nr4a3-deficient immune cells and uses thereof |
| WO2022256437A1 (en) | 2021-06-02 | 2022-12-08 | Lyell Immunopharma, Inc. | Nr4a3-deficient immune cells and uses thereof |
| US12139731B2 (en) | 2021-07-08 | 2024-11-12 | National Institutes Of Health (Nih), U.S. Dept. Of Health And Human Services (Dhhs) U.S. Government | CRISPR-based programmable RNA editing |
| EP4373963A4 (en) | 2021-07-21 | 2025-06-18 | Montana State University | NUCLEIC ACID DETECTION USING A TYPE III CRISPR COMPLEX |
| CN119452085A (zh) | 2021-08-27 | 2025-02-14 | 宏基因组学公司 | 具有ruvc结构域的酶 |
| WO2023039586A1 (en) | 2021-09-10 | 2023-03-16 | Agilent Technologies, Inc. | Guide rnas with chemical modification for prime editing |
| EP4596697A3 (en) | 2021-09-28 | 2025-08-27 | Acrigen Biosciences | Compositions and methods for nucleic acid modifications |
| WO2023052508A2 (en) | 2021-09-30 | 2023-04-06 | Astrazeneca Ab | Use of inhibitors to increase efficiency of crispr/cas insertions |
| KR20240082391A (ko) | 2021-10-14 | 2024-06-10 | 론자 세일즈 아게 | 세포외 소포 생산을 위한 변형된 생산자 세포 |
| EP4423271A2 (en) | 2021-10-28 | 2024-09-04 | Regeneron Pharmaceuticals, Inc. | Crispr/cas-related methods and compositions for knocking out c5 |
| EP4437096A4 (en) | 2021-11-24 | 2025-09-24 | Metagenomi Inc | ENDONUCLEASE SYSTEMS |
| CN118434849A (zh) * | 2021-11-24 | 2024-08-02 | 宏基因组学公司 | 核酸内切酶系统 |
| KR20240117571A (ko) | 2021-12-08 | 2024-08-01 | 리제너론 파마슈티칼스 인코포레이티드 | 돌연변이 마이오실린 질환 모델 및 이의 용도 |
| GB202118058D0 (en) | 2021-12-14 | 2022-01-26 | Univ Warwick | Methods to increase yields in crops |
| US20230279442A1 (en) | 2021-12-15 | 2023-09-07 | Versitech Limited | Engineered cas9-nucleases and method of use thereof |
| EP4447649A1 (en) | 2021-12-17 | 2024-10-23 | Keygene N.V. | Double decapitation of plants |
| CA3241882A1 (en) | 2021-12-29 | 2023-07-06 | Bristol-Myers Squibb Company | Generation of landing pad cell lines |
| WO2023150181A1 (en) | 2022-02-01 | 2023-08-10 | President And Fellows Of Harvard College | Methods and compositions for treating cancer |
| JP2025508677A (ja) | 2022-02-02 | 2025-04-10 | リジェネロン・ファーマシューティカルズ・インコーポレイテッド | ポンペ病の処置のための抗TfR:GAA及び抗CD63:GAAの挿入 |
| EP4514981A2 (en) | 2022-04-29 | 2025-03-05 | Regeneron Pharmaceuticals, Inc. | Identification of tissue-specific extragenic safe harbors for gene therapy approaches |
| WO2023220603A1 (en) | 2022-05-09 | 2023-11-16 | Regeneron Pharmaceuticals, Inc. | Vectors and methods for in vivo antibody production |
| CN119947735A (zh) | 2022-05-19 | 2025-05-06 | 莱尔免疫制药公司 | 靶向nr4a3的多核苷酸及其用途 |
| EP4532720A2 (en) | 2022-05-31 | 2025-04-09 | Regeneron Pharmaceuticals, Inc. | Crispr interference therapeutics for c9orf72 repeat expansion disease |
| WO2023235725A2 (en) | 2022-05-31 | 2023-12-07 | Regeneron Pharmaceuticals, Inc. | Crispr-based therapeutics for c9orf72 repeat expansion disease |
| GB202209518D0 (en) | 2022-06-29 | 2022-08-10 | Snipr Biome Aps | Treating & preventing E coli infections |
| GB2621813A (en) | 2022-06-30 | 2024-02-28 | Univ Newcastle | Preventing disease recurrence in Mitochondrial replacement therapy |
| AU2023314808A1 (en) | 2022-07-29 | 2025-03-20 | Regeneron Pharmaceuticals, Inc. | Compositions and methods for transferrin receptor (tfr)-mediated delivery to the brain and muscle |
| CA3261296A1 (en) | 2022-08-05 | 2024-02-08 | Regeneron Pharmaceuticals, Inc. | TDP-43 VARIANTS RESISTANT TO AGGREGATION |
| WO2024064952A1 (en) | 2022-09-23 | 2024-03-28 | Lyell Immunopharma, Inc. | Methods for culturing nr4a-deficient cells overexpressing c-jun |
| WO2024064958A1 (en) | 2022-09-23 | 2024-03-28 | Lyell Immunopharma, Inc. | Methods for culturing nr4a-deficient cells |
| WO2024073606A1 (en) | 2022-09-28 | 2024-04-04 | Regeneron Pharmaceuticals, Inc. | Antibody resistant modified receptors to enhance cell-based therapies |
| WO2024077174A1 (en) | 2022-10-05 | 2024-04-11 | Lyell Immunopharma, Inc. | Methods for culturing nr4a-deficient cells |
| JP7353602B1 (ja) | 2022-10-14 | 2023-10-02 | 株式会社インプランタイノベーションズ | ゲノム編集方法およびゲノム編集用組成物 |
| CN120077129A (zh) | 2022-10-21 | 2025-05-30 | 沃奇酶科基因组学公司 | 用于测序文库归一化的方法和组合物 |
| EP4612184A1 (en) | 2022-11-04 | 2025-09-10 | Regeneron Pharmaceuticals, Inc. | Calcium voltage-gated channel auxiliary subunit gamma 1 (cacng1) binding proteins and cacng1-mediated delivery to skeletal muscle |
| WO2024107765A2 (en) | 2022-11-14 | 2024-05-23 | Regeneron Pharmaceuticals, Inc. | Compositions and methods for fibroblast growth factor receptor 3-mediated delivery to astrocytes |
| JP2025542235A (ja) | 2022-12-22 | 2025-12-25 | キージーン ナムローゼ フェンノートシャップ | プロトプラストカルス接木による再生 |
| JP2026503704A (ja) | 2023-01-27 | 2026-01-29 | リジェネロン・ファーマシューティカルズ・インコーポレイテッド | 修飾されたラブドウイルス糖タンパク質およびその使用 |
| WO2024201368A1 (en) | 2023-03-29 | 2024-10-03 | Astrazeneca Ab | Use of inhibitors to increase efficiency of crispr/cas insertions |
| CN116617414B (zh) * | 2023-03-31 | 2024-04-05 | 中国农业大学 | 一种脂质体及其制备方法和应用 |
| KR20260026049A (ko) | 2023-06-15 | 2026-02-25 | 리제너론 파아마슈티컬스, 인크. | 청력 장애에 대한 유전자 치료 |
| AU2024309884A1 (en) | 2023-06-30 | 2025-12-18 | Regeneron Pharmaceuticals, Inc. | Methods and compositions for increasing homology-directed repair |
| AU2024315073A1 (en) | 2023-07-28 | 2026-01-22 | Regeneron Pharmaceuticals, Inc. | Use of bgh-sv40l tandem polya to enhance transgene expression during unidirectional gene insertion |
| AU2024317483A1 (en) | 2023-07-28 | 2026-01-29 | Regeneron Pharmaceuticals, Inc. | Anti-tfr:gaa and anti-cd63:gaa insertion for treatment of pompe disease |
| AR133384A1 (es) | 2023-07-28 | 2025-09-24 | Regeneron Pharma | Anti-tfr:esfingomielinasa ácida para el tratamiento de la deficiencia de esfingomielinasa ácida |
| WO2025038750A2 (en) | 2023-08-14 | 2025-02-20 | President And Fellows Of Harvard College | Methods and compositions for treating cancer |
| WO2025049524A1 (en) | 2023-08-28 | 2025-03-06 | Regeneron Pharmaceuticals, Inc. | Cxcr4 antibody-resistant modified receptors |
| WO2025049959A2 (en) | 2023-09-01 | 2025-03-06 | Renagade Therapeutics Management Inc. | Gene editing systems, compositions, and methods for treatment of vexas syndrome |
| WO2025064408A1 (en) | 2023-09-18 | 2025-03-27 | The Broad Institute, Inc. | Compositions and methods for treating cardiovascular disease |
| GB202314578D0 (en) | 2023-09-22 | 2023-11-08 | Univ Manchester | Methods of producing homoplasmic modified plants or parts thereof |
| WO2025081042A1 (en) | 2023-10-12 | 2025-04-17 | Renagade Therapeutics Management Inc. | Nickase-retron template-based precision editing system and methods of use |
| US20250276092A1 (en) | 2024-03-01 | 2025-09-04 | Regeneron Pharmaceuticals, Inc. | Methods and compositions for re-dosing aav using anti-cd40 antagonistic antibody to suppress host anti-aav antibody response |
| WO2025217398A1 (en) | 2024-04-10 | 2025-10-16 | Lyell Immunopharma, Inc. | Methods for culturing cells with improved culture medium |
| WO2025235388A1 (en) | 2024-05-06 | 2025-11-13 | Regeneron Pharmaceuticals, Inc. | Transgene genomic identification by nuclease-mediated long read sequencing |
| WO2025259669A1 (en) | 2024-06-10 | 2025-12-18 | Regeneron Pharmaceuticals, Inc. | Methods and systems for characterizing modified oligonucleotides |
| WO2025257212A1 (en) | 2024-06-11 | 2025-12-18 | Keygene N.V. | Screening and regeneration of protoplast callus |
| US20250388890A1 (en) | 2024-06-20 | 2025-12-25 | Regeneron Pharmaceuticals, Inc. | ASS1 Gene Insertion For The Treatment Of Citrullinemia Type I |
| WO2026006542A2 (en) | 2024-06-26 | 2026-01-02 | Yale University | Compositions and methods for crispr/cas9 based reactivation of human angelman syndrome |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110312792A (zh) * | 2016-12-14 | 2019-10-08 | 瓦赫宁根大学 | 热稳定的Cas9核酸酶 |
| WO2019217336A2 (en) * | 2018-05-06 | 2019-11-14 | Massachusetts Institute Of Technology | APPLICATIONS OF STREPTOCOCCUS-DERIVED Cas9 NUCLEASSES ON MINIMAL ADENINE-RICH PAM TARGETS |
| CN111448313A (zh) * | 2017-11-16 | 2020-07-24 | 阿斯利康(瑞典)有限公司 | 用于改善基于Cas9的敲入策略的有效性的组合物和方法 |
| CN112424824A (zh) * | 2018-07-11 | 2021-02-26 | 维尔纽斯大学 | 用于生物样品图像的计算机实现的方法 |
| WO2022261115A1 (en) * | 2021-06-07 | 2022-12-15 | Yale University | Peptide nucleic acids for spatiotemporal control of crispr-cas binding |
| WO2023025767A1 (en) * | 2021-08-23 | 2023-03-02 | Max-Delbrück-Centrum Für Molekulare Medizin In Der Helmholtz-Gemeinschaft | Method for cas9 nickase-mediated gene editing |
| CN116555254A (zh) * | 2015-10-09 | 2023-08-08 | 孟山都技术公司 | 新颖的rna导向性核酸酶及其用途 |
Family Cites Families (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3862002A (en) * | 1962-05-08 | 1975-01-21 | Sanfar Lab Inc | Production of physiologically active placental substances |
| CA2619833C (en) | 2005-08-26 | 2017-05-09 | Danisco A/S | Use of crispr associated genes (cas) |
| ES2719789T3 (es) | 2007-03-02 | 2019-07-16 | Dupont Nutrition Biosci Aps | Cultivos con resistencia mejorada a fagos |
| US20100076057A1 (en) | 2008-09-23 | 2010-03-25 | Northwestern University | TARGET DNA INTERFERENCE WITH crRNA |
| JP6208580B2 (ja) * | 2010-05-17 | 2017-10-04 | サンガモ セラピューティクス, インコーポレイテッド | 新規のdna結合タンパク質及びその使用 |
| EP2580331A4 (en) * | 2010-06-14 | 2013-11-27 | Univ Iowa State Res Found Inc | NUCLEASE ACTIVITY OF THE TAL EFFECTOR AND FUSION PROTEIN FOKI |
| CA2993567C (en) * | 2010-07-21 | 2022-06-28 | Sangamo Biosciences, Inc. | Methods and compositions for modification of a t-cell receptor gene |
| US9637739B2 (en) | 2012-03-20 | 2017-05-02 | Vilnius University | RNA-directed DNA cleavage by the Cas9-crRNA complex |
| WO2013141680A1 (en) | 2012-03-20 | 2013-09-26 | Vilnius University | RNA-DIRECTED DNA CLEAVAGE BY THE Cas9-crRNA COMPLEX |
| PL4289948T3 (pl) | 2012-05-25 | 2025-06-02 | The Regents Of The University Of California | Sposoby i kompozycje do modyfikacji kierowanego na rna docelowego dna i do nakierowanej na rna modulacji transkrypcji |
| EP2912175B1 (en) * | 2012-10-23 | 2018-08-22 | Toolgen Incorporated | Composition for cleaving a target dna comprising a guide rna specific for the target dna and cas protein-encoding nucleic acid or cas protein, and use thereof |
| CN105142669B (zh) | 2012-12-06 | 2018-07-03 | 西格马-奥尔德里奇有限责任公司 | 基于crispr的基因组修饰和调控 |
| CN105121648B (zh) * | 2012-12-12 | 2021-05-07 | 布罗德研究所有限公司 | 用于序列操纵的系统、方法和优化的指导组合物的工程化 |
| WO2014093622A2 (en) | 2012-12-12 | 2014-06-19 | The Broad Institute, Inc. | Delivery, engineering and optimization of systems, methods and compositions for sequence manipulation and therapeutic applications |
| US8697359B1 (en) | 2012-12-12 | 2014-04-15 | The Broad Institute, Inc. | CRISPR-Cas systems and methods for altering expression of gene products |
| CN105658796B (zh) | 2012-12-12 | 2021-10-26 | 布罗德研究所有限公司 | 用于序列操纵的crispr-cas组分系统、方法以及组合物 |
| WO2014093701A1 (en) * | 2012-12-12 | 2014-06-19 | The Broad Institute, Inc. | Functional genomics using crispr-cas systems, compositions, methods, knock out libraries and applications thereof |
| EP4282970A3 (en) | 2012-12-17 | 2024-01-17 | President and Fellows of Harvard College | Rna-guided human genome engineering |
| MY197877A (en) * | 2013-06-04 | 2023-07-22 | Harvard College | Rna-guided transcriptional regulation |
| US20150275231A1 (en) * | 2014-03-31 | 2015-10-01 | Mice With Horns, Llc | Method of Preventing or Reducing Virus Transmission in Animals |
-
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- 2013-03-15 US US14/385,241 patent/US9637739B2/en active Active
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Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116555254A (zh) * | 2015-10-09 | 2023-08-08 | 孟山都技术公司 | 新颖的rna导向性核酸酶及其用途 |
| CN110312792A (zh) * | 2016-12-14 | 2019-10-08 | 瓦赫宁根大学 | 热稳定的Cas9核酸酶 |
| CN111448313A (zh) * | 2017-11-16 | 2020-07-24 | 阿斯利康(瑞典)有限公司 | 用于改善基于Cas9的敲入策略的有效性的组合物和方法 |
| WO2019217336A2 (en) * | 2018-05-06 | 2019-11-14 | Massachusetts Institute Of Technology | APPLICATIONS OF STREPTOCOCCUS-DERIVED Cas9 NUCLEASSES ON MINIMAL ADENINE-RICH PAM TARGETS |
| CN112424824A (zh) * | 2018-07-11 | 2021-02-26 | 维尔纽斯大学 | 用于生物样品图像的计算机实现的方法 |
| WO2022261115A1 (en) * | 2021-06-07 | 2022-12-15 | Yale University | Peptide nucleic acids for spatiotemporal control of crispr-cas binding |
| WO2023025767A1 (en) * | 2021-08-23 | 2023-03-02 | Max-Delbrück-Centrum Für Molekulare Medizin In Der Helmholtz-Gemeinschaft | Method for cas9 nickase-mediated gene editing |
Non-Patent Citations (4)
| Title |
|---|
| CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III;CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III;《Nature》;第602-607页 * |
| CRISPR/Cas9基因编辑系统研究进展及其在动物基因编辑研究中的应用;王玮玮;刘瑞琪;吴勇延;杨严格;王勇胜;卿素珠;;畜牧兽医学报;20160719(第07期);全文 * |
| CRISPR-Cas9技术在医药领域中的应用;孙明尧;白玮;梁宏玉;赵秋蓉;张悦榕;徐慰倬;;沈阳药科大学学报;20191220(第12期);全文 * |
| The Streptococcus thermophilus CRISPR/Cas system provides immunity in Escherichia coli;Rimantas Sapranauskas;《Nucleic Acids Research》;第9275-9282页 * |
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