JP6423338B2 - Cas9−crRNA複合体によるRNA指向性DNA切断 - Google Patents
Cas9−crRNA複合体によるRNA指向性DNA切断 Download PDFInfo
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- JP6423338B2 JP6423338B2 JP2015501880A JP2015501880A JP6423338B2 JP 6423338 B2 JP6423338 B2 JP 6423338B2 JP 2015501880 A JP2015501880 A JP 2015501880A JP 2015501880 A JP2015501880 A JP 2015501880A JP 6423338 B2 JP6423338 B2 JP 6423338B2
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- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
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- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
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- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/35—Nature of the modification
- C12N2310/351—Conjugate
- C12N2310/3513—Protein; Peptide
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/50—Physical structure
- C12N2310/53—Physical structure partially self-complementary or closed
- C12N2310/531—Stem-loop; Hairpin
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- C12N2800/00—Nucleic acids vectors
- C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
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- Peptides Or Proteins (AREA)
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Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261613373P | 2012-03-20 | 2012-03-20 | |
| US61/613,373 | 2012-03-20 | ||
| US201261625420P | 2012-04-17 | 2012-04-17 | |
| US61/625,420 | 2012-04-17 | ||
| PCT/US2013/033106 WO2013142578A1 (en) | 2012-03-20 | 2013-03-20 | RNA-DIRECTED DNA CLEAVAGE BY THE Cas9-crRNA COMPLEX |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018196574A Division JP7186574B2 (ja) | 2012-03-20 | 2018-10-18 | Cas9-crRNA複合体によるRNA指向性DNA切断 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015510778A JP2015510778A (ja) | 2015-04-13 |
| JP2015510778A5 JP2015510778A5 (enExample) | 2015-10-08 |
| JP6423338B2 true JP6423338B2 (ja) | 2018-11-14 |
Family
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Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015501880A Active JP6423338B2 (ja) | 2012-03-20 | 2013-03-20 | Cas9−crRNA複合体によるRNA指向性DNA切断 |
| JP2018196574A Active JP7186574B2 (ja) | 2012-03-20 | 2018-10-18 | Cas9-crRNA複合体によるRNA指向性DNA切断 |
| JP2020174803A Active JP7113877B2 (ja) | 2012-03-20 | 2020-10-16 | Cas9-crRNA複合体によるRNA指向性DNA切断 |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018196574A Active JP7186574B2 (ja) | 2012-03-20 | 2018-10-18 | Cas9-crRNA複合体によるRNA指向性DNA切断 |
| JP2020174803A Active JP7113877B2 (ja) | 2012-03-20 | 2020-10-16 | Cas9-crRNA複合体によるRNA指向性DNA切断 |
Country Status (15)
| Country | Link |
|---|---|
| US (8) | US9637739B2 (enExample) |
| EP (2) | EP2828386B1 (enExample) |
| JP (3) | JP6423338B2 (enExample) |
| CN (2) | CN114410625B (enExample) |
| BR (1) | BR112014023353A2 (enExample) |
| CA (2) | CA3124374C (enExample) |
| DK (1) | DK2828386T3 (enExample) |
| EA (1) | EA029544B1 (enExample) |
| ES (1) | ES2749108T3 (enExample) |
| HU (1) | HUE046295T2 (enExample) |
| IN (1) | IN2014DN07846A (enExample) |
| MX (2) | MX370435B (enExample) |
| PL (1) | PL2828386T3 (enExample) |
| PT (1) | PT2828386T (enExample) |
| WO (1) | WO2013142578A1 (enExample) |
Families Citing this family (432)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2798703A1 (en) | 2010-05-10 | 2011-11-17 | The Regents Of The University Of California | Endoribonuclease compositions and methods of use thereof |
| US11951140B2 (en) | 2011-02-04 | 2024-04-09 | Seed Health, Inc. | Modulation of an individual's gut microbiome to address osteoporosis and bone disease |
| US11191665B2 (en) | 2011-02-04 | 2021-12-07 | Joseph E. Kovarik | Method and system for reducing the likelihood of a porphyromonas gingivalis infection in a human being |
| US11523934B2 (en) | 2011-02-04 | 2022-12-13 | Seed Health, Inc. | Method and system to facilitate the growth of desired bacteria in a human's mouth |
| US10687975B2 (en) | 2011-02-04 | 2020-06-23 | Joseph E. Kovarik | Method and system to facilitate the growth of desired bacteria in a human's mouth |
| US12279989B2 (en) | 2011-02-04 | 2025-04-22 | Seed Health, Inc. | Method and system for increasing beneficial bacteria and decreasing pathogenic bacteria in the oral cavity |
| US11844720B2 (en) | 2011-02-04 | 2023-12-19 | Seed Health, Inc. | Method and system to reduce the likelihood of dental caries and halitosis |
| US10583033B2 (en) | 2011-02-04 | 2020-03-10 | Katherine Rose Kovarik | Method and system for reducing the likelihood of a porphyromonas gingivalis infection in a human being |
| US12257272B2 (en) | 2015-12-24 | 2025-03-25 | Seed Health, Inc. | Method and system for reducing the likelihood of developing depression in an individual |
| US11951139B2 (en) | 2015-11-30 | 2024-04-09 | Seed Health, Inc. | Method and system for reducing the likelihood of osteoporosis |
| US11998479B2 (en) | 2011-02-04 | 2024-06-04 | Seed Health, Inc. | Method and system for addressing adverse effects on the oral microbiome and restoring gingival health caused by sodium lauryl sulphate exposure |
| BR112013024337A2 (pt) | 2011-03-23 | 2017-09-26 | Du Pont | locus de traço transgênico complexo em uma planta, planta ou semente, método para produzir em uma planta um locus de traço transgênico complexo e construto de expressão |
| CA2853829C (en) | 2011-07-22 | 2023-09-26 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| JP2014531908A (ja) | 2011-10-14 | 2014-12-04 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 構造アッセンブリによる配列決定 |
| ES2991004T3 (es) | 2011-12-22 | 2024-12-02 | Harvard College | Métodos para la detección de analitos |
| GB201122458D0 (en) | 2011-12-30 | 2012-02-08 | Univ Wageningen | Modified cascade ribonucleoproteins and uses thereof |
| US9637739B2 (en) | 2012-03-20 | 2017-05-02 | Vilnius University | RNA-directed DNA cleavage by the Cas9-crRNA complex |
| US9834786B2 (en) | 2012-04-25 | 2017-12-05 | Regeneron Pharmaceuticals, Inc. | Nuclease-mediated targeting with large targeting vectors |
| US10266850B2 (en) * | 2012-05-25 | 2019-04-23 | The Regents Of The University Of California | Methods and compositions for RNA-directed target DNA modification and for RNA-directed modulation of transcription |
| KR20230065381A (ko) * | 2012-07-25 | 2023-05-11 | 더 브로드 인스티튜트, 인코퍼레이티드 | 유도 dna 결합 단백질 및 게놈 교란 도구 및 이의 적용 |
| CN110669758A (zh) | 2012-10-23 | 2020-01-10 | 基因工具股份有限公司 | 用于切割靶dna 的组合物及其用途 |
| CA3034794A1 (en) | 2012-12-06 | 2014-06-12 | Sigma-Aldrich Co. Llc | Crispr-based genome modification and regulation |
| US8697359B1 (en) * | 2012-12-12 | 2014-04-15 | The Broad Institute, Inc. | CRISPR-Cas systems and methods for altering expression of gene products |
| EP3434776A1 (en) | 2012-12-12 | 2019-01-30 | The Broad Institute, Inc. | Methods, models, systems, and apparatus for identifying target sequences for cas enzymes or crispr-cas systems for target sequences and conveying results thereof |
| CN105121648B (zh) | 2012-12-12 | 2021-05-07 | 布罗德研究所有限公司 | 用于序列操纵的系统、方法和优化的指导组合物的工程化 |
| WO2014093694A1 (en) * | 2012-12-12 | 2014-06-19 | The Broad Institute, Inc. | Crispr-cas nickase systems, methods and compositions for sequence manipulation in eukaryotes |
| WO2014093701A1 (en) | 2012-12-12 | 2014-06-19 | The Broad Institute, Inc. | Functional genomics using crispr-cas systems, compositions, methods, knock out libraries and applications thereof |
| RU2721275C2 (ru) | 2012-12-12 | 2020-05-18 | Те Брод Инститьют, Инк. | Доставка, конструирование и оптимизация систем, способов и композиций для манипуляции с последовательностями и применения в терапии |
| EP4234696A3 (en) | 2012-12-12 | 2023-09-06 | The Broad Institute Inc. | Crispr-cas component systems, methods and compositions for sequence manipulation |
| AU2013359212B2 (en) | 2012-12-12 | 2017-01-19 | Massachusetts Institute Of Technology | Engineering and optimization of improved systems, methods and enzyme compositions for sequence manipulation |
| WO2014093655A2 (en) | 2012-12-12 | 2014-06-19 | The Broad Institute, Inc. | Engineering and optimization of systems, methods and compositions for sequence manipulation with functional domains |
| EP2931891B1 (en) | 2012-12-17 | 2019-05-15 | President and Fellows of Harvard College | Rna-guided human genome engineering |
| CA2898184A1 (en) | 2013-01-16 | 2014-07-24 | Emory University | Cas9-nucleic acid complexes and uses related thereto |
| EP2971184B1 (en) | 2013-03-12 | 2019-04-17 | President and Fellows of Harvard College | Method of generating a three-dimensional nucleic acid containing matrix |
| DK3620534T3 (da) | 2013-03-14 | 2021-12-06 | Caribou Biosciences Inc | Crispr-cas sammensætninger af nucleinsyre-targeting nucleinsyrer |
| KR102271291B1 (ko) | 2013-03-15 | 2021-07-02 | 더 제너럴 하스피탈 코포레이션 | 특정 게놈 좌위에 대한 유전적 및 후성적 조절 단백질의 rna-안내 표적화 |
| US10760064B2 (en) | 2013-03-15 | 2020-09-01 | The General Hospital Corporation | RNA-guided targeting of genetic and epigenomic regulatory proteins to specific genomic loci |
| US9902973B2 (en) | 2013-04-11 | 2018-02-27 | Caribou Biosciences, Inc. | Methods of modifying a target nucleic acid with an argonaute |
| LT2986729T (lt) | 2013-04-16 | 2018-10-25 | Regeneron Pharmaceuticals, Inc. | Žiurkės genomo tikslinė modifikacija |
| US9873907B2 (en) | 2013-05-29 | 2018-01-23 | Agilent Technologies, Inc. | Method for fragmenting genomic DNA using CAS9 |
| WO2014191518A1 (en) * | 2013-05-29 | 2014-12-04 | Cellectis | A method for producing precise dna cleavage using cas9 nickase activity |
| AU2014274939B2 (en) | 2013-06-04 | 2020-03-19 | President And Fellows Of Harvard College | RNA-guideded transcriptional regulation |
| US9267135B2 (en) | 2013-06-04 | 2016-02-23 | President And Fellows Of Harvard College | RNA-guided transcriptional regulation |
| EP3725885A1 (en) | 2013-06-17 | 2020-10-21 | The Broad Institute, Inc. | Functional genomics using crispr-cas systems, compositions methods, screens and applications thereof |
| CA2915837A1 (en) | 2013-06-17 | 2014-12-24 | The Broad Institute, Inc. | Optimized crispr-cas double nickase systems, methods and compositions for sequence manipulation |
| WO2014204729A1 (en) | 2013-06-17 | 2014-12-24 | The Broad Institute Inc. | Delivery, use and therapeutic applications of the crispr-cas systems and compositions for targeting disorders and diseases using viral components |
| EP3011029B1 (en) * | 2013-06-17 | 2019-12-11 | The Broad Institute, Inc. | Delivery, engineering and optimization of tandem guide systems, methods and compositions for sequence manipulation |
| BR112015031608A2 (pt) | 2013-06-17 | 2017-08-22 | Massachusetts Inst Technology | Aplicação e uso dos sistemas crispr-cas, vetores e composições para direcionamento e terapia hepáticos |
| US10011850B2 (en) | 2013-06-21 | 2018-07-03 | The General Hospital Corporation | Using RNA-guided FokI Nucleases (RFNs) to increase specificity for RNA-Guided Genome Editing |
| BR112016000571B1 (pt) | 2013-07-10 | 2023-12-26 | President And Fellows Of Harvard College | Métodos in vitro para modular a expressão e para alterar um ou mais ácidos nucleicos alvo em uma célula simultaneamente com a regulação da expressão de um ou mais ácidos nucleicos alvo em uma célula, bem como célula de levedura ou bactéria compreendendo ácidos nucleicos |
| US10563225B2 (en) | 2013-07-26 | 2020-02-18 | President And Fellows Of Harvard College | Genome engineering |
| US10421957B2 (en) | 2013-07-29 | 2019-09-24 | Agilent Technologies, Inc. | DNA assembly using an RNA-programmable nickase |
| US9163284B2 (en) | 2013-08-09 | 2015-10-20 | President And Fellows Of Harvard College | Methods for identifying a target site of a Cas9 nuclease |
| BR112016003591A8 (pt) | 2013-08-22 | 2018-01-30 | Du Pont | promotor u6 de polimerase iii de soja e métodos de uso |
| US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
| US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
| US9340800B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | Extended DNA-sensing GRNAS |
| US9526784B2 (en) * | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
| ES3013744T3 (en) | 2013-09-18 | 2025-04-15 | Kymab Ltd | Methods, cells and organisms |
| WO2015065964A1 (en) | 2013-10-28 | 2015-05-07 | The Broad Institute Inc. | Functional genomics using crispr-cas systems, compositions, methods, screens and applications thereof |
| WO2015066119A1 (en) | 2013-10-30 | 2015-05-07 | North Carolina State University | Compositions and methods related to a type-ii crispr-cas system in lactobacillus buchneri |
| EP3066201B1 (en) | 2013-11-07 | 2018-03-07 | Editas Medicine, Inc. | Crispr-related methods and compositions with governing grnas |
| WO2015070193A1 (en) * | 2013-11-11 | 2015-05-14 | Liu Oliver | Compositions and methods for targeted gene disruption in prokaryotes |
| US10787684B2 (en) * | 2013-11-19 | 2020-09-29 | President And Fellows Of Harvard College | Large gene excision and insertion |
| US9074199B1 (en) | 2013-11-19 | 2015-07-07 | President And Fellows Of Harvard College | Mutant Cas9 proteins |
| WO2015075056A1 (en) * | 2013-11-19 | 2015-05-28 | Thermo Fisher Scientific Baltics Uab | Programmable enzymes for isolation of specific dna fragments |
| CA2928635C (en) | 2013-11-28 | 2022-06-21 | Horizon Genomics Gmbh | Somatic haploid human cell line |
| LT3080279T (lt) | 2013-12-11 | 2018-10-25 | Regeneron Pharmaceuticals, Inc. | Būdai ir kompozicijos, skirti genomo tikslinei modifikacijai |
| CN110951779B (zh) | 2013-12-11 | 2024-04-16 | 瑞泽恩制药公司 | 用于靶向修饰基因组的方法和组合物 |
| AU2014361834B2 (en) | 2013-12-12 | 2020-10-22 | Massachusetts Institute Of Technology | CRISPR-Cas systems and methods for altering expression of gene products, structural information and inducible modular Cas enzymes |
| EP3080257A1 (en) | 2013-12-12 | 2016-10-19 | The Broad Institute Inc. | Compositions and methods of use of crispr-cas systems in nucleotide repeat disorders |
| MX374529B (es) | 2013-12-12 | 2025-03-06 | Broad Inst Inc | Suministro, uso y aplicaciones terapeuticas de sistemas y composiciones crispr-cas para edicion del genoma. |
| US9840699B2 (en) | 2013-12-12 | 2017-12-12 | President And Fellows Of Harvard College | Methods for nucleic acid editing |
| EP3080261B1 (en) | 2013-12-12 | 2019-05-22 | The Broad Institute, Inc. | Delivery, use and therapeutic applications of the crispr-cas systems and compositions for hbv and viral diseases and disorders |
| WO2015089486A2 (en) | 2013-12-12 | 2015-06-18 | The Broad Institute Inc. | Systems, methods and compositions for sequence manipulation with optimized functional crispr-cas systems |
| WO2015089364A1 (en) | 2013-12-12 | 2015-06-18 | The Broad Institute Inc. | Crystal structure of a crispr-cas system, and uses thereof |
| EP4219699A1 (en) | 2013-12-12 | 2023-08-02 | The Broad Institute, Inc. | Engineering of systems, methods and optimized guide compositions with new architectures for sequence manipulation |
| US9476065B2 (en) | 2013-12-19 | 2016-10-25 | Amyris, Inc. | Methods for genomic integration |
| US11833177B2 (en) | 2013-12-20 | 2023-12-05 | Seed Health, Inc. | Probiotic to enhance an individual's skin microbiome |
| US11839632B2 (en) | 2013-12-20 | 2023-12-12 | Seed Health, Inc. | Topical application of CRISPR-modified bacteria to treat acne vulgaris |
| US11672835B2 (en) | 2013-12-20 | 2023-06-13 | Seed Health, Inc. | Method for treating individuals having cancer and who are receiving cancer immunotherapy |
| US11998574B2 (en) | 2013-12-20 | 2024-06-04 | Seed Health, Inc. | Method and system for modulating an individual's skin microbiome |
| US11980643B2 (en) | 2013-12-20 | 2024-05-14 | Seed Health, Inc. | Method and system to modify an individual's gut-brain axis to provide neurocognitive protection |
| US11969445B2 (en) | 2013-12-20 | 2024-04-30 | Seed Health, Inc. | Probiotic composition and method for controlling excess weight, obesity, NAFLD and NASH |
| US12329783B2 (en) | 2013-12-20 | 2025-06-17 | Seed Health, Inc. | Method and system to improve the health of a person's skin microbiome |
| US11642382B2 (en) | 2013-12-20 | 2023-05-09 | Seed Health, Inc. | Method for treating an individual suffering from bladder cancer |
| US12005085B2 (en) | 2013-12-20 | 2024-06-11 | Seed Health, Inc. | Probiotic method and composition for maintaining a healthy vaginal microbiome |
| US11826388B2 (en) | 2013-12-20 | 2023-11-28 | Seed Health, Inc. | Topical application of Lactobacillus crispatus to ameliorate barrier damage and inflammation |
| US12246043B2 (en) | 2013-12-20 | 2025-03-11 | Seed Health, Inc. | Topical application to treat acne vulgaris |
| US11529379B2 (en) | 2013-12-20 | 2022-12-20 | Seed Health, Inc. | Method and system for reducing the likelihood of developing colorectal cancer in an individual human being |
| US12318377B2 (en) | 2013-12-20 | 2025-06-03 | Seed Health, Inc. | Method and system for reducing the likelihood of a porphyromonas gingivalis infection in a human being |
| EP3089989B1 (en) * | 2013-12-31 | 2020-06-24 | The Regents of The University of California | Cas9 crystals and methods of use thereof |
| MX2016009243A (es) * | 2014-01-14 | 2017-03-08 | Lam Therapeutics Inc | Métodos de mutagénesis. |
| US10787654B2 (en) | 2014-01-24 | 2020-09-29 | North Carolina State University | Methods and compositions for sequence guiding Cas9 targeting |
| DE212015000061U1 (de) | 2014-02-11 | 2017-09-03 | The Regents Of The University Of Colorado, A Body Corporate | CRISPR-ermöglichtes Multiplex Genom Engineering |
| CN113265394B (zh) | 2014-02-13 | 2024-08-06 | 宝生物工程(美国)有限公司 | 从核酸的初始集合中耗尽靶分子的方法、以及用于实践其的组合物和试剂盒 |
| CA2940084A1 (en) | 2014-02-18 | 2015-08-27 | Duke University | Compositions for the inactivation of virus replication and methods of making and using the same |
| WO2015134812A1 (en) | 2014-03-05 | 2015-09-11 | Editas Medicine, Inc. | Crispr/cas-related methods and compositions for treating usher syndrome and retinitis pigmentosa |
| DK3116997T3 (da) | 2014-03-10 | 2019-08-19 | Editas Medicine Inc | Crispr/cas-relaterede fremgangsmåder og sammensætninger til behandling af lebers kongenitale amaurose 10 (lca10) |
| US11339437B2 (en) | 2014-03-10 | 2022-05-24 | Editas Medicine, Inc. | Compositions and methods for treating CEP290-associated disease |
| US11141493B2 (en) | 2014-03-10 | 2021-10-12 | Editas Medicine, Inc. | Compositions and methods for treating CEP290-associated disease |
| EP3981876A1 (en) | 2014-03-26 | 2022-04-13 | Editas Medicine, Inc. | Crispr/cas-related methods and compositions for treating sickle cell disease |
| EP3126495A1 (en) | 2014-04-02 | 2017-02-08 | Editas Medicine, Inc. | Crispr/cas-related methods and compositions for treating primary open angle glaucoma |
| JP2017512481A (ja) | 2014-04-08 | 2017-05-25 | ノースカロライナ ステート ユニバーシティーNorth Carolina State University | Crispr関連遺伝子を用いた、rna依存性の転写抑制のための方法および組成物 |
| GB201406968D0 (en) * | 2014-04-17 | 2014-06-04 | Green Biologics Ltd | Deletion mutants |
| GB201406970D0 (en) | 2014-04-17 | 2014-06-04 | Green Biologics Ltd | Targeted mutations |
| EP3150718A4 (en) * | 2014-05-28 | 2018-03-28 | Toolgen Incorporated | Method for sensitive detection of target dna using target-specific nuclease |
| WO2015184268A1 (en) | 2014-05-30 | 2015-12-03 | The Board Of Trustees Of The Leland Stanford Junior University | Compositions and methods of delivering treatments for latent viral infections |
| SG11201609634YA (en) | 2014-06-06 | 2016-12-29 | Regeneron Pharma | Methods and compositions for modifying a targeted locus |
| JP6784601B2 (ja) | 2014-06-23 | 2020-11-11 | ザ ジェネラル ホスピタル コーポレイション | シークエンシングによって評価されるゲノムワイドでバイアスのないDSBの同定(GUIDE−Seq) |
| US9738897B2 (en) * | 2014-06-23 | 2017-08-22 | Regeneron Pharmaceuticals, Inc. | Nuclease-mediated DNA assembly |
| PL3161128T3 (pl) | 2014-06-26 | 2019-02-28 | Regeneron Pharmaceuticals, Inc. | Sposoby i kompozycje do celowanych modyfikacji genetycznych i sposoby zastosowania |
| CA2954626A1 (en) | 2014-07-11 | 2016-01-14 | E. I. Du Pont De Nemours And Company | Compositions and methods for producing plants resistant to glyphosate herbicide |
| WO2016007839A1 (en) | 2014-07-11 | 2016-01-14 | President And Fellows Of Harvard College | Methods for high-throughput labelling and detection of biological features in situ using microscopy |
| WO2016022363A2 (en) | 2014-07-30 | 2016-02-11 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
| CN113789317B (zh) * | 2014-08-06 | 2024-02-23 | 基因工具股份有限公司 | 使用空肠弯曲杆菌crispr/cas系统衍生的rna引导的工程化核酸酶的基因编辑 |
| CN107429241B (zh) | 2014-08-14 | 2025-10-24 | 百奥赛图(北京)医药科技股份有限公司 | Dna敲入系统 |
| CA2958292A1 (en) * | 2014-08-19 | 2016-02-25 | President And Fellows Of Harvard College | Rna-guided systems for probing and mapping of nucleic acids |
| US9970030B2 (en) | 2014-08-27 | 2018-05-15 | Caribou Biosciences, Inc. | Methods for increasing CAS9-mediated engineering efficiency |
| US10450584B2 (en) | 2014-08-28 | 2019-10-22 | North Carolina State University | Cas9 proteins and guiding features for DNA targeting and genome editing |
| US10570418B2 (en) | 2014-09-02 | 2020-02-25 | The Regents Of The University Of California | Methods and compositions for RNA-directed target DNA modification |
| US11560568B2 (en) | 2014-09-12 | 2023-01-24 | E. I. Du Pont De Nemours And Company | Generation of site-specific-integration sites for complex trait loci in corn and soybean, and methods of use |
| CN113016623A (zh) | 2014-09-26 | 2021-06-25 | 谱赛科美国股份有限公司 | 用于甜菊的单核苷酸多态性(snp)标志物 |
| CA2963693A1 (en) | 2014-10-10 | 2016-04-14 | Editas Medicine, Inc. | Compositions and methods for promoting homology directed repair |
| DK3207124T3 (da) | 2014-10-15 | 2019-08-12 | Regeneron Pharma | Fremgangsmåder og sammensætninger til generering eller bevaring af pluripotente celler |
| JP6779217B2 (ja) | 2014-10-24 | 2020-11-04 | アヴェクタス リミテッド | 細胞原形質膜を越える送達方法 |
| US9816080B2 (en) | 2014-10-31 | 2017-11-14 | President And Fellows Of Harvard College | Delivery of CAS9 via ARRDC1-mediated microvesicles (ARMMs) |
| AU2015342749B2 (en) | 2014-11-07 | 2022-01-27 | Editas Medicine, Inc. | Methods for improving CRISPR/Cas-mediated genome-editing |
| KR20160059994A (ko) | 2014-11-19 | 2016-05-27 | 기초과학연구원 | 두 개의 벡터로부터 발현된 Cas9 단백질을 이용한 유전자 발현 조절 방법 |
| CA3176380A1 (en) | 2014-11-21 | 2016-05-26 | Regeneron Pharmaceuticals, Inc. | Methods and compositions for targeted genetic modification using paired guide rnas |
| CA2969619A1 (en) * | 2014-12-03 | 2016-06-09 | Agilent Technologies, Inc. | Guide rna with chemical modifications |
| JP6830437B2 (ja) | 2014-12-10 | 2021-02-17 | リージェンツ オブ ザ ユニバーシティ オブ ミネソタ | 疾患を処置するための遺伝子改変された細胞、組織および臓器 |
| EP3985115A1 (en) | 2014-12-12 | 2022-04-20 | The Broad Institute, Inc. | Protected guide rnas (pgrnas) |
| PL3234134T3 (pl) * | 2014-12-17 | 2020-12-28 | Proqr Therapeutics Ii B.V. | Ukierunkowana edycja rna |
| EP3653048B9 (en) | 2014-12-19 | 2023-10-04 | Regeneron Pharmaceuticals, Inc. | Methods and compositions for targeted genetic modification through single-step multiple targeting |
| WO2016100857A1 (en) | 2014-12-19 | 2016-06-23 | Regeneron Pharmaceuticals, Inc. | Stem cells for modeling type 2 diabetes |
| AU2015364286B2 (en) * | 2014-12-20 | 2021-11-04 | Arc Bio, Llc | Compositions and methods for targeted depletion, enrichment, and partitioning of nucleic acids using CRISPR/Cas system proteins |
| EP3702456A1 (en) | 2014-12-24 | 2020-09-02 | The Broad Institute, Inc. | Crispr having or associated with destabilization domains |
| US11339399B2 (en) | 2014-12-31 | 2022-05-24 | Viridos, Inc. | Compositions and methods for high efficiency in vivo genome editing |
| WO2016112351A1 (en) | 2015-01-09 | 2016-07-14 | Bio-Rad Laboratories, Inc. | Detection of genome editing |
| CN105821073B (zh) | 2015-01-27 | 2021-08-10 | 中国科学院遗传与发育生物学研究所 | 一种通过基因瞬时表达对整株植物定点改造的方法 |
| RU2713328C2 (ru) | 2015-01-28 | 2020-02-04 | Пайонир Хай-Бред Интернэшнл, Инк. | Гибридные днк/рнк-полинуклеотиды crispr и способы применения |
| US9944912B2 (en) | 2015-03-03 | 2018-04-17 | The General Hospital Corporation | Engineered CRISPR-Cas9 nucleases with altered PAM specificity |
| EA038896B1 (ru) | 2015-03-16 | 2021-11-03 | Институт Генетики И Биологии Развития Академии Наук Китая | Способ осуществления сайт-направленной модификации растительных геномов с использованием ненаследуемых материалов |
| CN107567499A (zh) | 2015-03-27 | 2018-01-09 | 纳幕尔杜邦公司 | 大豆u6核小rna基因启动子及其在植物小rna基因的组成型表达中的用途 |
| AU2016246450B2 (en) | 2015-04-06 | 2022-03-17 | Agilent Technologies, Inc. | Chemically modified guide RNAs for CRISPR/Cas-mediated gene regulation |
| US11180793B2 (en) | 2015-04-24 | 2021-11-23 | Editas Medicine, Inc. | Evaluation of Cas9 molecule/guide RNA molecule complexes |
| CN108026566A (zh) | 2015-05-04 | 2018-05-11 | 特拉维夫大学拉莫特有限公司 | 用于使dna片段化的方法和试剂盒 |
| ES2905525T3 (es) | 2015-05-06 | 2022-04-11 | Snipr Tech Ltd | Alteración de poblaciones microbianas y modificación de la microbiota |
| EP3294896A1 (en) | 2015-05-11 | 2018-03-21 | Editas Medicine, Inc. | Optimized crispr/cas9 systems and methods for gene editing in stem cells |
| AU2016265560B2 (en) * | 2015-05-15 | 2022-07-28 | Pioneer Hi-Bred International, Inc. | Rapid characterization of Cas endonuclease systems, PAM sequences and guide RNA elements |
| WO2016186745A1 (en) * | 2015-05-15 | 2016-11-24 | Ge Healthcare Dharmacon, Inc. | Synthetic single guide rna for cas9-mediated gene editing |
| EP3095870A1 (en) | 2015-05-19 | 2016-11-23 | Kws Saat Se | Methods for the in planta transformation of plants and manufacturing processes and products based and obtainable therefrom |
| US10117911B2 (en) | 2015-05-29 | 2018-11-06 | Agenovir Corporation | Compositions and methods to treat herpes simplex virus infections |
| US10136649B2 (en) | 2015-05-29 | 2018-11-27 | North Carolina State University | Methods for screening bacteria, archaea, algae, and yeast using CRISPR nucleic acids |
| WO2016196282A1 (en) | 2015-05-29 | 2016-12-08 | Agenovir Corporation | Compositions and methods for cell targeted hpv treatment |
| US10883103B2 (en) | 2015-06-02 | 2021-01-05 | Monsanto Technology Llc | Compositions and methods for delivery of a polynucleotide into a plant |
| WO2016196887A1 (en) | 2015-06-03 | 2016-12-08 | Board Of Regents Of The University Of Nebraska | Dna editing using single-stranded dna |
| EP3303634B1 (en) | 2015-06-03 | 2023-08-30 | The Regents of The University of California | Cas9 variants and methods of use thereof |
| KR102796744B1 (ko) | 2015-06-09 | 2025-04-15 | 에디타스 메디신, 인코포레이티드 | 이식의 개선을 위한 crispr/cas-관련 방법 및 조성물 |
| KR20180034389A (ko) | 2015-06-12 | 2018-04-04 | 론자 워커스빌 아이엔씨. | 합성 전사인자를 이용한 핵 역분화 방법 |
| CN115537421A (zh) | 2015-06-15 | 2022-12-30 | 北卡罗来纳州立大学 | 有效递送核酸和基于rna的抗微生物剂的方法和组合物 |
| WO2016205759A1 (en) | 2015-06-18 | 2016-12-22 | The Broad Institute Inc. | Engineering and optimization of systems, methods, enzymes and guide scaffolds of cas9 orthologs and variants for sequence manipulation |
| US9790490B2 (en) * | 2015-06-18 | 2017-10-17 | The Broad Institute Inc. | CRISPR enzymes and systems |
| WO2016205613A1 (en) | 2015-06-18 | 2016-12-22 | The Broad Institute Inc. | Crispr enzyme mutations reducing off-target effects |
| EP4545544A3 (en) | 2015-06-29 | 2025-10-08 | Ionis Pharmaceuticals, Inc. | Modified crispr rna and modified single crispr rna and uses thereof |
| CA2993431A1 (en) | 2015-07-31 | 2017-02-09 | Regents Of The University Of Minnesota | Nuclease based knockouts of immunological checkpoint genes in immune cells |
| US9580727B1 (en) | 2015-08-07 | 2017-02-28 | Caribou Biosciences, Inc. | Compositions and methods of engineered CRISPR-Cas9 systems using split-nexus Cas9-associated polynucleotides |
| CN106467909A (zh) | 2015-08-14 | 2017-03-01 | 中国科学院遗传与发育生物学研究所 | 一种通过核苷酸定点替换获得抗草甘膦水稻的方法 |
| CN108138176B (zh) | 2015-08-19 | 2022-05-24 | 阿克生物公司 | 使用基于核酸引导的核酸酶的系统捕获核酸 |
| US9512446B1 (en) | 2015-08-28 | 2016-12-06 | The General Hospital Corporation | Engineered CRISPR-Cas9 nucleases |
| US9926546B2 (en) | 2015-08-28 | 2018-03-27 | The General Hospital Corporation | Engineered CRISPR-Cas9 nucleases |
| JP6799586B2 (ja) | 2015-08-28 | 2020-12-16 | ザ ジェネラル ホスピタル コーポレイション | 遺伝子操作CRISPR−Cas9ヌクレアーゼ |
| WO2017044419A1 (en) | 2015-09-08 | 2017-03-16 | University Of Massachusetts | Dnase h activity of neisseria meningitidis cas9 |
| KR20180043369A (ko) | 2015-09-11 | 2018-04-27 | 더 제너럴 하스피탈 코포레이션 | 뉴클레아제 dsb의 완전한 호출 및 시퀀싱(find-seq) |
| CA2999500A1 (en) | 2015-09-24 | 2017-03-30 | Editas Medicine, Inc. | Use of exonucleases to improve crispr/cas-mediated genome editing |
| WO2017058751A1 (en) | 2015-09-28 | 2017-04-06 | North Carolina State University | Methods and compositions for sequence specific antimicrobials |
| US9850484B2 (en) | 2015-09-30 | 2017-12-26 | The General Hospital Corporation | Comprehensive in vitro reporting of cleavage events by sequencing (Circle-seq) |
| CN108367081A (zh) | 2015-10-14 | 2018-08-03 | 生命技术公司 | 核糖核蛋白转染剂 |
| AU2016342038B2 (en) * | 2015-10-22 | 2022-09-08 | Massachusetts Institute Of Technology | Type VI-B CRISPR enzymes and systems |
| ES2699848T3 (es) | 2015-10-23 | 2019-02-13 | Caribou Biosciences Inc | Acido nucleico CRISPR clase 2 de tipo cruzado modificado que se dirige a ácidos nucleicos |
| IL294014B2 (en) | 2015-10-23 | 2024-07-01 | Harvard College | Nucleobase editors and uses thereof |
| GB2559526B (en) | 2015-11-03 | 2021-02-17 | Harvard College | Method and apparatus for volumetric imaging of a three-dimensional nucleic acid containing matrix |
| WO2017079428A1 (en) | 2015-11-04 | 2017-05-11 | President And Fellows Of Harvard College | Site specific germline modification |
| WO2017081288A1 (en) | 2015-11-11 | 2017-05-18 | Lonza Ltd | Crispr-associated (cas) proteins with reduced immunogenicity |
| KR101906491B1 (ko) * | 2015-11-30 | 2018-12-05 | 기초과학연구원 | F. novicida 유래 Cas9을 포함하는 유전체 교정용 조성물 |
| EP3564371B1 (en) | 2015-12-04 | 2020-05-27 | Caribou Biosciences, Inc. | Engineered nucleic-acid targeting nucleic acids |
| US11208649B2 (en) | 2015-12-07 | 2021-12-28 | Zymergen Inc. | HTP genomic engineering platform |
| US11293029B2 (en) | 2015-12-07 | 2022-04-05 | Zymergen Inc. | Promoters from Corynebacterium glutamicum |
| US9988624B2 (en) | 2015-12-07 | 2018-06-05 | Zymergen Inc. | Microbial strain improvement by a HTP genomic engineering platform |
| US11542466B2 (en) | 2015-12-22 | 2023-01-03 | North Carolina State University | Methods and compositions for delivery of CRISPR based antimicrobials |
| JP7449646B2 (ja) * | 2015-12-30 | 2024-03-14 | アヴェクタス リミテッド | 細胞および組織への遺伝子編集タンパク質および組成物の、ベクターなしでの送達 |
| EA201891614A1 (ru) | 2016-01-11 | 2019-02-28 | Те Борд Оф Трастиз Оф Те Лилэнд Стэнфорд Джуниор Юниверсити | Химерные белки и способы иммунотерапии |
| NZ743983A (en) | 2016-01-11 | 2025-08-29 | Univ Leland Stanford Junior | Chimeric proteins and methods of regulating gene expression |
| JP2019508063A (ja) | 2016-01-27 | 2019-03-28 | オンコラス, インコーポレイテッド | 腫瘍溶解性ウイルスベクター及びその使用 |
| WO2017142999A2 (en) * | 2016-02-18 | 2017-08-24 | President And Fellows Of Harvard College | Methods and systems of molecular recording by crispr-cas system |
| WO2017147278A1 (en) | 2016-02-25 | 2017-08-31 | The Children's Medical Center Corporation | Customized class switch of immunoglobulin genes in lymphoma and hybridoma by crispr/cas9 technology |
| JP2019507610A (ja) * | 2016-03-04 | 2019-03-22 | インドア バイオテクノロジーズ インコーポレイテッド | CRISPR−Casゲノム編集に基づく、Fel d1ノックアウト並びに関連組成物及び方法 |
| WO2017165862A1 (en) | 2016-03-25 | 2017-09-28 | Editas Medicine, Inc. | Systems and methods for treating alpha 1-antitrypsin (a1at) deficiency |
| US11597924B2 (en) | 2016-03-25 | 2023-03-07 | Editas Medicine, Inc. | Genome editing systems comprising repair-modulating enzyme molecules and methods of their use |
| EP3443086B1 (en) | 2016-04-13 | 2021-11-24 | Editas Medicine, Inc. | Cas9 fusion molecules, gene editing systems, and methods of use thereof |
| AU2017257624B2 (en) | 2016-04-25 | 2023-05-25 | President And Fellows Of Harvard College | Hybridization chain reaction methods for in situ molecular detection |
| CN105907785B (zh) * | 2016-05-05 | 2020-02-07 | 苏州吉玛基因股份有限公司 | 化学合成的crRNA用于CRISPR/Cpf1系统在基因编辑中的应用 |
| EP3458595A2 (en) | 2016-05-18 | 2019-03-27 | Amyris, Inc. | Compositions and methods for genomic integration of nucleic acids into exogenous landing pads |
| CN109475109B (zh) | 2016-05-20 | 2021-10-29 | 瑞泽恩制药公司 | 用于使用多个引导rna来破坏免疫耐受性的方法 |
| GB2582731B8 (en) | 2016-06-02 | 2021-10-27 | Sigma Aldrich Co Llc | Using programmable DNA binding proteins to enhance targeted genome modification |
| GB201609811D0 (en) | 2016-06-05 | 2016-07-20 | Snipr Technologies Ltd | Methods, cells, systems, arrays, RNA and kits |
| US10767175B2 (en) | 2016-06-08 | 2020-09-08 | Agilent Technologies, Inc. | High specificity genome editing using chemically modified guide RNAs |
| US11293021B1 (en) | 2016-06-23 | 2022-04-05 | Inscripta, Inc. | Automated cell processing methods, modules, instruments, and systems |
| LT3474669T (lt) | 2016-06-24 | 2022-06-10 | The Regents Of The University Of Colorado, A Body Corporate | Barkodu pažymėtų kombinatorinių bibliotekų generavimo būdai |
| EP3474849B1 (en) | 2016-06-27 | 2025-05-21 | The Broad Institute, Inc. | Compositions and methods for detecting and treating diabetes |
| WO2018005793A1 (en) | 2016-06-30 | 2018-01-04 | Zymergen Inc. | Methods for generating a glucose permease library and uses thereof |
| EP3478833A4 (en) | 2016-06-30 | 2019-10-02 | Zymergen, Inc. | METHOD FOR PRODUCING A BACTERIAL HEMOGLOBIN LIBRARY AND ITS USE |
| EP3490373B1 (en) | 2016-07-29 | 2023-10-25 | Regeneron Pharmaceuticals, Inc. | Mice comprising mutations resulting in expression of c-truncated fibrillin-1 |
| BR112019001887A2 (pt) | 2016-08-02 | 2019-07-09 | Editas Medicine Inc | composições e métodos para o tratamento de doença associada a cep290 |
| AU2017306676B2 (en) | 2016-08-03 | 2024-02-22 | President And Fellows Of Harvard College | Adenosine nucleobase editors and uses thereof |
| US11078481B1 (en) | 2016-08-03 | 2021-08-03 | KSQ Therapeutics, Inc. | Methods for screening for cancer targets |
| EP3497214B1 (en) | 2016-08-09 | 2023-06-28 | President and Fellows of Harvard College | Programmable cas9-recombinase fusion proteins and uses thereof |
| US11542509B2 (en) | 2016-08-24 | 2023-01-03 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| US11078483B1 (en) | 2016-09-02 | 2021-08-03 | KSQ Therapeutics, Inc. | Methods for measuring and improving CRISPR reagent function |
| US20190225974A1 (en) | 2016-09-23 | 2019-07-25 | BASF Agricultural Solutions Seed US LLC | Targeted genome optimization in plants |
| WO2018067546A1 (en) | 2016-10-03 | 2018-04-12 | President And Fellows Of Harvard College | Delivery of therapeutic rnas via arrdc1-mediated microvesicles |
| WO2018071868A1 (en) | 2016-10-14 | 2018-04-19 | President And Fellows Of Harvard College | Aav delivery of nucleobase editors |
| EP3525832A4 (en) | 2016-10-14 | 2020-04-29 | The General Hospital Corp. | Epigenetically regulated site-specific nucleases |
| GB201617559D0 (en) | 2016-10-17 | 2016-11-30 | University Court Of The University Of Edinburgh The | Swine comprising modified cd163 and associated methods |
| EP4338799A3 (en) | 2016-10-18 | 2024-06-05 | Regents of the University of Minnesota | Tumor infiltrating lymphocytes and methods of therapy |
| CN109906030B (zh) | 2016-11-04 | 2022-03-18 | 安健基因公司 | 用于产生仅重链抗体的经基因修饰的非人动物和方法 |
| US9816093B1 (en) | 2016-12-06 | 2017-11-14 | Caribou Biosciences, Inc. | Engineered nucleic acid-targeting nucleic acids |
| WO2018209712A1 (en) * | 2017-05-19 | 2018-11-22 | Tsinghua University | Engineering of a minimal sacas9 crispr/cas system for gene editing and transcriptional regulation optimized by enhanced guide rna |
| EP3555297A1 (en) | 2016-12-19 | 2019-10-23 | Editas Medicine, Inc. | Assessing nuclease cleavage |
| WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
| EP3565907B1 (en) | 2017-01-06 | 2022-05-04 | Editas Medicine, Inc. | Methods of assessing nuclease cleavage |
| US20230190958A1 (en) | 2017-01-13 | 2023-06-22 | Jichi Medical University | AAV Vector for Disrupting Coagulation Factor-Related Gene on Liver Genome |
| CN108314736B (zh) * | 2017-01-18 | 2021-08-31 | 李燕强 | 一种促进rna降解的方法 |
| WO2018136702A1 (en) | 2017-01-23 | 2018-07-26 | Regeneron Pharmaceuticals, Inc. | Hydroxysteroid 17-beta dehydrogenase 13 (hsd17b13) variants and uses thereof |
| WO2018165631A1 (en) | 2017-03-09 | 2018-09-13 | President And Fellows Of Harvard College | Cancer vaccine |
| EP3592853A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Suppression of pain by gene editing |
| WO2018165629A1 (en) | 2017-03-10 | 2018-09-13 | President And Fellows Of Harvard College | Cytosine to guanine base editor |
| WO2018170184A1 (en) | 2017-03-14 | 2018-09-20 | Editas Medicine, Inc. | Systems and methods for the treatment of hemoglobinopathies |
| KR20240116572A (ko) | 2017-03-23 | 2024-07-29 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 핵산 프로그램가능한 dna 결합 단백질을 포함하는 핵염기 편집제 |
| US11913015B2 (en) | 2017-04-17 | 2024-02-27 | University Of Maryland, College Park | Embryonic cell cultures and methods of using the same |
| EP3612023A4 (en) | 2017-04-20 | 2021-05-12 | Egenesis, Inc. | Methods for generating genetically modified animals |
| AU2018254619B2 (en) | 2017-04-21 | 2022-07-21 | The General Hospital Corporation | Variants of Cpf1 (CAS12a) with altered PAM specificity |
| EP3615552A1 (en) | 2017-04-24 | 2020-03-04 | DuPont Nutrition Biosciences ApS | Methods and compositions of anti-crispr proteins for use in plants |
| EP3615672A1 (en) | 2017-04-28 | 2020-03-04 | Editas Medicine, Inc. | Methods and systems for analyzing guide rna molecules |
| WO2018209158A2 (en) | 2017-05-10 | 2018-11-15 | Editas Medicine, Inc. | Crispr/rna-guided nuclease systems and methods |
| US11560566B2 (en) | 2017-05-12 | 2023-01-24 | President And Fellows Of Harvard College | Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation |
| WO2018218166A1 (en) | 2017-05-25 | 2018-11-29 | The General Hospital Corporation | Using split deaminases to limit unwanted off-target base editor deamination |
| CN110914423B (zh) | 2017-05-31 | 2024-02-06 | 国立大学法人东京大学 | 经修饰的Cas9蛋白及其用途 |
| EP3635112A2 (en) | 2017-06-06 | 2020-04-15 | Zymergen, Inc. | A htp genomic engineering platform for improving fungal strains |
| JP2020524490A (ja) | 2017-06-06 | 2020-08-20 | ザイマージェン インコーポレイテッド | Escherichia Coliを改良するためのHTPゲノム操作プラットフォーム |
| CA3065813A1 (en) | 2017-06-09 | 2018-12-13 | Editas Medicine, Inc. | Engineered cas9 nucleases |
| MX2019014516A (es) | 2017-06-20 | 2020-01-23 | Jiangsu Hengrui Medicine Co | Metodo para eliminar el gen objetivo en celulas t in vitro y arncr utilizado en el metodo. |
| US9982279B1 (en) | 2017-06-23 | 2018-05-29 | Inscripta, Inc. | Nucleic acid-guided nucleases |
| US10011849B1 (en) | 2017-06-23 | 2018-07-03 | Inscripta, Inc. | Nucleic acid-guided nucleases |
| EP3645021A4 (en) | 2017-06-30 | 2021-04-21 | Intima Bioscience, Inc. | ADENO-ASSOCIATED VIRAL VECTORS FOR GENE THERAPY |
| RS63202B1 (sr) | 2017-06-30 | 2022-06-30 | Inscripta Inc | Automatizovani postupci, moduli, instrumenti i sistemi za obradu ćelija |
| EP3652312A1 (en) | 2017-07-14 | 2020-05-20 | Editas Medicine, Inc. | Systems and methods for targeted integration and genome editing and detection thereof using integrated priming sites |
| SG11202000691XA (en) | 2017-07-26 | 2020-02-27 | Oncorus Inc | Oncolytic viral vectors and uses thereof |
| CN111801345A (zh) | 2017-07-28 | 2020-10-20 | 哈佛大学的校长及成员们 | 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物 |
| BR112019027673A2 (pt) | 2017-07-31 | 2020-09-15 | Regeneron Pharmaceuticals, Inc. | animal não humano, e, métodos para testar a recombinação induzida por crispr/cas e para otimizar a capacidade de crispr/cas |
| CN115074343A (zh) | 2017-07-31 | 2022-09-20 | 瑞泽恩制药公司 | Cas转基因小鼠胚胎干细胞和小鼠及其应用 |
| WO2019028023A2 (en) | 2017-07-31 | 2019-02-07 | Regeneron Pharmaceuticals, Inc. | METHODS AND COMPOSITIONS FOR EVALUATING CRISPR / CAS MEDIATED DISRUPTION OR EXCISION AND CRISPR / CAS INDUCED RECOMBINATION USING IN VIVO EXOGENIC DONOR NUCLEIC ACID |
| CN111278450B (zh) | 2017-08-23 | 2024-04-09 | 通用医疗公司 | 具有改变的PAM特异性的工程化的CRISPR-Cas9核酸酶 |
| US10738327B2 (en) | 2017-08-28 | 2020-08-11 | Inscripta, Inc. | Electroporation cuvettes for automation |
| US11319532B2 (en) | 2017-08-30 | 2022-05-03 | President And Fellows Of Harvard College | High efficiency base editors comprising Gam |
| US11530396B2 (en) | 2017-09-05 | 2022-12-20 | The University Of Tokyo | Modified CAS9 protein, and use thereof |
| AU2018338790B2 (en) | 2017-09-29 | 2022-09-15 | Regeneron Pharmaceuticals, Inc. | Non-human animals comprising a humanized TTR locus and methods of use |
| CN111372650A (zh) | 2017-09-30 | 2020-07-03 | 因思科瑞普特公司 | 流通式电穿孔仪器 |
| US11725228B2 (en) | 2017-10-11 | 2023-08-15 | The General Hospital Corporation | Methods for detecting site-specific and spurious genomic deamination induced by base editing technologies |
| WO2019079347A1 (en) | 2017-10-16 | 2019-04-25 | The Broad Institute, Inc. | USES OF BASIC EDITORS ADENOSINE |
| CN120310773A (zh) | 2017-11-16 | 2025-07-15 | 阿斯利康(瑞典)有限公司 | 用于改善基于Cas9的敲入策略的有效性的组合物和方法 |
| US12406749B2 (en) | 2017-12-15 | 2025-09-02 | The Broad Institute, Inc. | Systems and methods for predicting repair outcomes in genetic engineering |
| BR112020014168A2 (pt) | 2018-01-12 | 2020-12-08 | Basf Se | Proteína, ácido nucleico isolado, gene recombinante, vetor, célula hospedeira, planta, parte de planta ou semente de trigo, métodos para produzir, produto de trigo, farinha, farelo integral, amido, grânulos de amido ou farelo de trigo e métodos para identificar e/ou selecionar uma planta de trigo |
| US20220010293A1 (en) | 2018-02-23 | 2022-01-13 | Pioneer Hi-Bred International, Inc. | Novel cas9 orthologs |
| WO2019178426A1 (en) | 2018-03-14 | 2019-09-19 | Editas Medicine, Inc. | Systems and methods for the treatment of hemoglobinopathies |
| EP3592140A1 (en) | 2018-03-19 | 2020-01-15 | Regeneron Pharmaceuticals, Inc. | Transcription modulation in animals using crispr/cas systems |
| US10760075B2 (en) | 2018-04-30 | 2020-09-01 | Snipr Biome Aps | Treating and preventing microbial infections |
| WO2019190874A1 (en) | 2018-03-29 | 2019-10-03 | Inscripta, Inc. | Automated control of cell growth rates for induction and transformation |
| AU2019247490A1 (en) | 2018-04-06 | 2020-10-22 | Children's Medical Center Corporation | Compositions and methods for somatic cell reprogramming and modulating imprinting |
| WO2019200004A1 (en) | 2018-04-13 | 2019-10-17 | Inscripta, Inc. | Automated cell processing instruments comprising reagent cartridges |
| CA3097044A1 (en) | 2018-04-17 | 2019-10-24 | The General Hospital Corporation | Sensitive in vitro assays for substrate preferences and sites of nucleic acid binding, modifying, and cleaving agents |
| US10526598B2 (en) | 2018-04-24 | 2020-01-07 | Inscripta, Inc. | Methods for identifying T-cell receptor antigens |
| WO2019209926A1 (en) | 2018-04-24 | 2019-10-31 | Inscripta, Inc. | Automated instrumentation for production of peptide libraries |
| US10858761B2 (en) | 2018-04-24 | 2020-12-08 | Inscripta, Inc. | Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells |
| WO2019222545A1 (en) | 2018-05-16 | 2019-11-21 | Synthego Corporation | Methods and systems for guide rna design and use |
| EP3797160A1 (en) | 2018-05-23 | 2021-03-31 | The Broad Institute Inc. | Base editors and uses thereof |
| EP3823633A4 (en) | 2018-06-29 | 2023-05-03 | Editas Medicine, Inc. | SYNTHETIC LEAD MOLECULES, COMPOSITIONS AND METHODS RELATED THERETO |
| WO2020014261A1 (en) | 2018-07-09 | 2020-01-16 | The Broad Institute, Inc. | Rna programmable epigenetic rna modifiers and uses thereof |
| IL292273B2 (en) | 2018-08-14 | 2023-10-01 | Inscripta Inc | Devices, modules and methods for improved detection of edited sequences in living cells |
| US11142740B2 (en) | 2018-08-14 | 2021-10-12 | Inscripta, Inc. | Detection of nuclease edited sequences in automated modules and instruments |
| US10752874B2 (en) | 2018-08-14 | 2020-08-25 | Inscripta, Inc. | Instruments, modules, and methods for improved detection of edited sequences in live cells |
| US10532324B1 (en) | 2018-08-14 | 2020-01-14 | Inscripta, Inc. | Instruments, modules, and methods for improved detection of edited sequences in live cells |
| BR112021003545A2 (pt) | 2018-08-29 | 2021-05-18 | Amyris, Inc. | células e métodos para ensaio baseado em seleção |
| US11965154B2 (en) | 2018-08-30 | 2024-04-23 | Inscripta, Inc. | Detection of nuclease edited sequences in automated modules and instruments |
| EP3847251A1 (en) | 2018-09-07 | 2021-07-14 | Astrazeneca AB | Compositions and methods for improved nucleases |
| US12203123B2 (en) | 2018-10-01 | 2025-01-21 | North Carolina State University | Recombinant type I CRISPR-Cas system and uses thereof for screening for variant cells |
| WO2020072254A1 (en) | 2018-10-01 | 2020-04-09 | North Carolina State University | Recombinant type i crispr-cas system and uses thereof for killing target cells |
| WO2020072250A1 (en) | 2018-10-01 | 2020-04-09 | North Carolina State University | Recombinant type i crispr-cas system and uses thereof for genome modification and alteration of expression |
| US10711267B2 (en) | 2018-10-01 | 2020-07-14 | North Carolina State University | Recombinant type I CRISPR-Cas system |
| WO2020076976A1 (en) | 2018-10-10 | 2020-04-16 | Readcoor, Inc. | Three-dimensional spatial molecular indexing |
| US11851663B2 (en) | 2018-10-14 | 2023-12-26 | Snipr Biome Aps | Single-vector type I vectors |
| US11214781B2 (en) | 2018-10-22 | 2022-01-04 | Inscripta, Inc. | Engineered enzyme |
| EP3870697A4 (en) | 2018-10-22 | 2022-11-09 | Inscripta, Inc. | GMO ENZYMES |
| WO2020092453A1 (en) | 2018-10-29 | 2020-05-07 | The Broad Institute, Inc. | Nucleobase editors comprising geocas9 and uses thereof |
| MX2021005028A (es) | 2018-10-31 | 2021-08-24 | Zymergen Inc | Ensamble determinista multiplexado de genotecas de adn. |
| US20220010321A1 (en) | 2018-11-01 | 2022-01-13 | Keygene N.V. | Dual guide rna for crispr/cas genome editing in plants cells |
| WO2020109412A1 (en) | 2018-11-28 | 2020-06-04 | Keygene N.V. | Targeted enrichment by endonuclease protection |
| KR20200071198A (ko) | 2018-12-10 | 2020-06-19 | 네오이뮨텍, 인코퍼레이티드 | Nrf2 발현 조절 기반 T 세포 항암면역치료법 |
| BR112021011372A2 (pt) | 2018-12-14 | 2021-08-31 | Pioneer Hi-Bred International, Inc. | Novos sistemas crispr-cas para edição de genoma |
| SG11202105189RA (en) | 2018-12-20 | 2021-06-29 | Regeneron Pharma | Nuclease-mediated repeat expansion |
| WO2020154500A1 (en) | 2019-01-23 | 2020-07-30 | The Broad Institute, Inc. | Supernegatively charged proteins and uses thereof |
| US11946040B2 (en) | 2019-02-04 | 2024-04-02 | The General Hospital Corporation | Adenine DNA base editor variants with reduced off-target RNA editing |
| EP3924477A4 (en) * | 2019-02-14 | 2023-03-29 | Metagenomi, Inc. | ENZYMES WITH RUVC DOMAINS |
| US10982200B2 (en) | 2019-02-14 | 2021-04-20 | Metagenomi Ip Technologies, Llc | Enzymes with RuvC domains |
| US11053515B2 (en) | 2019-03-08 | 2021-07-06 | Zymergen Inc. | Pooled genome editing in microbes |
| CN113728106A (zh) | 2019-03-08 | 2021-11-30 | 齐默尔根公司 | 微生物中的迭代基因组编辑 |
| CN120648640A (zh) | 2019-03-18 | 2025-09-16 | 瑞泽恩制药公司 | 用于鉴定tau接种或聚集的基因修饰因子的CRISPR/Cas筛选平台 |
| ES2969026T3 (es) | 2019-03-18 | 2024-05-16 | Regeneron Pharma | Plataforma de detección de abandonos CRISPR/CAS para revelar vulnerabilidades genéticas asociadas con la agregación de tau |
| BR112021018607A2 (pt) | 2019-03-19 | 2021-11-23 | Massachusetts Inst Technology | Métodos e composições para editar sequências de nucleotídeos |
| US10815467B2 (en) | 2019-03-25 | 2020-10-27 | Inscripta, Inc. | Simultaneous multiplex genome editing in yeast |
| US11001831B2 (en) | 2019-03-25 | 2021-05-11 | Inscripta, Inc. | Simultaneous multiplex genome editing in yeast |
| EP3947425A1 (en) | 2019-03-27 | 2022-02-09 | Pioneer Hi-Bred International, Inc. | Plant explant transformation |
| CN118064502A (zh) | 2019-04-03 | 2024-05-24 | 瑞泽恩制药公司 | 用于将抗体编码序列插入到安全港基因座中的方法和组合物 |
| CN113795588B (zh) | 2019-04-04 | 2025-02-25 | 瑞泽恩制药公司 | 用于在靶向性载体中无瘢痕引入靶向修饰的方法 |
| KR102661779B1 (ko) | 2019-04-04 | 2024-04-30 | 리제너론 파마슈티칼스 인코포레이티드 | 인간화 응고 인자 12 좌위를 포함하는 비-인간 동물 |
| WO2020204159A1 (ja) | 2019-04-05 | 2020-10-08 | 国立大学法人大阪大学 | ノックイン細胞の作製方法 |
| EP4530351A3 (en) | 2019-04-12 | 2025-06-18 | Astrazeneca AB | Compositions and methods for improved gene editing |
| EP3956349A1 (en) | 2019-04-17 | 2022-02-23 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| KR20220016869A (ko) | 2019-06-04 | 2022-02-10 | 리제너론 파마슈티칼스 인코포레이티드 | 베타-슬립 돌연변이를 갖는 인간화 ttr 좌위를 포함하는 비-인간 동물 및 사용 방법 |
| CN113939593A (zh) | 2019-06-06 | 2022-01-14 | 因思科瑞普特公司 | 用于递归的核酸指导的细胞编辑的处治 |
| CN113939595A (zh) | 2019-06-07 | 2022-01-14 | 瑞泽恩制药公司 | 包括人源化白蛋白基因座的非人动物 |
| EP3813522B1 (en) | 2019-06-14 | 2025-05-07 | Regeneron Pharmaceuticals, Inc. | Models of tauopathy |
| US10907125B2 (en) | 2019-06-20 | 2021-02-02 | Inscripta, Inc. | Flow through electroporation modules and instrumentation |
| US10920189B2 (en) | 2019-06-21 | 2021-02-16 | Inscripta, Inc. | Genome-wide rationally-designed mutations leading to enhanced lysine production in E. coli |
| US10927385B2 (en) | 2019-06-25 | 2021-02-23 | Inscripta, Inc. | Increased nucleic-acid guided cell editing in yeast |
| KR20220044212A (ko) | 2019-08-09 | 2022-04-06 | 넛크래커 테라퓨틱스 인코포레이티드 | 미세유체 장치 및 이의 사용 방법 |
| AU2020346056A1 (en) | 2019-09-13 | 2022-03-31 | Regeneron Pharmaceuticals, Inc. | Transcription modulation in animals using CRISPR/Cas systems delivered by lipid nanoparticles |
| KR20220108036A (ko) | 2019-09-26 | 2022-08-02 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 최소 아레스틴 도메인 함유 단백질 1 (arrdc1) 구축물 |
| US12435330B2 (en) | 2019-10-10 | 2025-10-07 | The Broad Institute, Inc. | Methods and compositions for prime editing RNA |
| EP4054651A1 (en) | 2019-11-08 | 2022-09-14 | Regeneron Pharmaceuticals, Inc. | Crispr and aav strategies for x-linked juvenile retinoschisis therapy |
| WO2021102059A1 (en) | 2019-11-19 | 2021-05-27 | Inscripta, Inc. | Methods for increasing observed editing in bacteria |
| WO2021108363A1 (en) | 2019-11-25 | 2021-06-03 | Regeneron Pharmaceuticals, Inc. | Crispr/cas-mediated upregulation of humanized ttr allele |
| US10883095B1 (en) | 2019-12-10 | 2021-01-05 | Inscripta, Inc. | Mad nucleases |
| US10704033B1 (en) | 2019-12-13 | 2020-07-07 | Inscripta, Inc. | Nucleic acid-guided nucleases |
| JP2023507566A (ja) | 2019-12-18 | 2023-02-24 | インスクリプタ, インコーポレイテッド | 核酸誘導ヌクレアーゼ編集済み細胞のin vivo検出のためのカスケード/dCas3相補性アッセイ |
| US10689669B1 (en) | 2020-01-11 | 2020-06-23 | Inscripta, Inc. | Automated multi-module cell processing methods, instruments, and systems |
| US12312613B2 (en) | 2020-01-24 | 2025-05-27 | The General Hospital Corporation | Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants |
| US12264341B2 (en) | 2020-01-24 | 2025-04-01 | The General Hospital Corporation | CRISPR-Cas enzymes with enhanced on-target activity |
| KR20220133257A (ko) | 2020-01-27 | 2022-10-04 | 인스크립타 인코포레이티드 | 전기천공 모듈 및 기구 |
| AU2021232598A1 (en) | 2020-03-04 | 2022-09-08 | Regeneron Pharmaceuticals, Inc. | Methods and compositions for sensitization of tumor cells to immune therapy |
| EP4119166A4 (en) | 2020-03-12 | 2024-06-05 | Institute for Basic Science | COMPOSITION FOR INDUCING APOPTOSIS OF CELLS HAVING GENOMIC SEQUENCE VARIATION AND METHOD FOR INDUCING APOPTOSIS OF CELLS USING THE COMPOSITION |
| WO2021195079A1 (en) | 2020-03-23 | 2021-09-30 | Regeneron Pharmaceuticals, Inc. | Non-human animals comprising a humanized ttr locus comprising a v30m mutation and methods of use |
| WO2021202568A1 (en) | 2020-03-31 | 2021-10-07 | Metagenomi Ip Technologies, Llc | Class ii, type ii crispr systems |
| WO2021202938A1 (en) | 2020-04-03 | 2021-10-07 | Creyon Bio, Inc. | Oligonucleotide-based machine learning |
| US20210332388A1 (en) | 2020-04-24 | 2021-10-28 | Inscripta, Inc. | Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells |
| EP4146797A1 (en) | 2020-05-06 | 2023-03-15 | Orchard Therapeutics (Europe) Limited | Treatment for neurodegenerative diseases |
| AU2021267940A1 (en) | 2020-05-08 | 2022-12-08 | President And Fellows Of Harvard College | Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence |
| US11787841B2 (en) | 2020-05-19 | 2023-10-17 | Inscripta, Inc. | Rationally-designed mutations to the thrA gene for enhanced lysine production in E. coli |
| US20220049303A1 (en) | 2020-08-17 | 2022-02-17 | Readcoor, Llc | Methods and systems for spatial mapping of genetic variants |
| WO2022060749A1 (en) | 2020-09-15 | 2022-03-24 | Inscripta, Inc. | Crispr editing to embed nucleic acid landing pads into genomes of live cells |
| EP4225914A1 (en) | 2020-10-06 | 2023-08-16 | Keygene N.V. | Targeted sequence addition |
| US20230416709A1 (en) | 2020-11-06 | 2023-12-28 | Editforce, Inc. | Foki nuclease domain mutant |
| US11512297B2 (en) | 2020-11-09 | 2022-11-29 | Inscripta, Inc. | Affinity tag for recombination protein recruitment |
| JP2023548979A (ja) | 2020-11-10 | 2023-11-21 | インダストリアル マイクロブス, インコーポレイテッド | 供給原料からポリ(hiba)を産生可能な微生物 |
| WO2022112316A1 (en) | 2020-11-24 | 2022-06-02 | Keygene N.V. | Targeted enrichment using nanopore selective sequencing |
| WO2022120022A1 (en) | 2020-12-02 | 2022-06-09 | Regeneron Pharmaceuticals, Inc. | Crispr sam biosensor cell lines and methods of use thereof |
| AU2021415461A1 (en) | 2021-01-04 | 2023-08-17 | Inscripta, Inc. | Mad nucleases |
| US20240376451A1 (en) | 2021-01-07 | 2024-11-14 | Inscripta, Inc. | Mad nucleases |
| US11884924B2 (en) | 2021-02-16 | 2024-01-30 | Inscripta, Inc. | Dual strand nucleic acid-guided nickase editing |
| CN117136003A (zh) | 2021-04-15 | 2023-11-28 | 主基因有限公司 | 共再生顽拗性植物 |
| EP4323529A1 (en) | 2021-04-15 | 2024-02-21 | Keygene N.V. | Mobile endonucleases for heritable mutations |
| EP4337769A1 (en) | 2021-05-10 | 2024-03-20 | SQZ Biotechnologies Company | Methods for delivering genome editing molecules to the nucleus or cytosol of a cell and uses thereof |
| EP4347805A1 (en) | 2021-05-27 | 2024-04-10 | Astrazeneca AB | Cas9 effector proteins with enhanced stability |
| WO2022251644A1 (en) | 2021-05-28 | 2022-12-01 | Lyell Immunopharma, Inc. | Nr4a3-deficient immune cells and uses thereof |
| EP4347826A1 (en) | 2021-06-02 | 2024-04-10 | Lyell Immunopharma, Inc. | Nr4a3-deficient immune cells and uses thereof |
| EP4367239A4 (en) | 2021-07-08 | 2025-07-23 | Univ Montana State | CRISPR-BASED PROGRAMMABLE RNA EDITING |
| WO2023004391A2 (en) | 2021-07-21 | 2023-01-26 | Montana State University | Nucleic acid detection using type iii crispr complex |
| MX2024002539A (es) | 2021-08-27 | 2024-03-19 | Metagenomi Inc | Enzimas con dominios ruvc. |
| CA3230927A1 (en) | 2021-09-10 | 2023-03-16 | Agilent Technologies, Inc. | Guide rnas with chemical modification for prime editing |
| EP4408995A4 (en) | 2021-09-28 | 2025-08-13 | Acrigen Biosciences | COMPOSITIONS AND METHODS FOR NUCLEIC ACID MODIFICATIONS |
| EP4408996A2 (en) | 2021-09-30 | 2024-08-07 | Astrazeneca AB | Use of inhibitors to increase efficiency of crispr/cas insertions |
| WO2023064924A1 (en) | 2021-10-14 | 2023-04-20 | Codiak Biosciences, Inc. | Modified producer cells for extracellular vesicle production |
| US20240415980A1 (en) | 2021-10-28 | 2024-12-19 | Regeneron Pharmaceuticals, Inc. | Crispr/cas-related methods and compositions for knocking out c5 |
| CN118434849A (zh) * | 2021-11-24 | 2024-08-02 | 宏基因组学公司 | 核酸内切酶系统 |
| WO2023097282A1 (en) | 2021-11-24 | 2023-06-01 | Metagenomi, Inc. | Endonuclease systems |
| AU2022408167A1 (en) | 2021-12-08 | 2024-06-06 | Regeneron Pharmaceuticals, Inc. | Mutant myocilin disease model and uses thereof |
| GB202118058D0 (en) | 2021-12-14 | 2022-01-26 | Univ Warwick | Methods to increase yields in crops |
| US20230279442A1 (en) | 2021-12-15 | 2023-09-07 | Versitech Limited | Engineered cas9-nucleases and method of use thereof |
| WO2023111225A1 (en) | 2021-12-17 | 2023-06-22 | Keygene N.V. | Double decapitation of plants |
| JP2025501221A (ja) | 2021-12-29 | 2025-01-17 | ブリストル-マイヤーズ スクイブ カンパニー | ランディングパッド細胞株の生成 |
| WO2023150181A1 (en) | 2022-02-01 | 2023-08-10 | President And Fellows Of Harvard College | Methods and compositions for treating cancer |
| US20250032642A1 (en) | 2022-02-02 | 2025-01-30 | Regeneron Pharmaceuticals, Inc. | Crispr-mediated transgene insertion in neonatal cells |
| JP2025514304A (ja) | 2022-04-29 | 2025-05-02 | リジェネロン・ファーマシューティカルズ・インコーポレイテッド | 遺伝子治療法のための組織特異的遺伝子外セーフハーバーの同定 |
| JP2025516527A (ja) | 2022-05-09 | 2025-05-30 | リジェネロン・ファーマシューティカルズ・インコーポレイテッド | インビボ抗体産生のためのベクター及び方法 |
| AU2023273885A1 (en) | 2022-05-19 | 2024-12-05 | Lyell Immunopharma, Inc. | Polynucleotides targeting nr4a3 and uses thereof |
| WO2023235726A2 (en) | 2022-05-31 | 2023-12-07 | Regeneron Pharmaceuticals, Inc. | Crispr interference therapeutics for c9orf72 repeat expansion disease |
| WO2023235725A2 (en) | 2022-05-31 | 2023-12-07 | Regeneron Pharmaceuticals, Inc. | Crispr-based therapeutics for c9orf72 repeat expansion disease |
| GB202209518D0 (en) | 2022-06-29 | 2022-08-10 | Snipr Biome Aps | Treating & preventing E coli infections |
| GB2621813A (en) | 2022-06-30 | 2024-02-28 | Univ Newcastle | Preventing disease recurrence in Mitochondrial replacement therapy |
| WO2024026474A1 (en) | 2022-07-29 | 2024-02-01 | Regeneron Pharmaceuticals, Inc. | Compositions and methods for transferrin receptor (tfr)-mediated delivery to the brain and muscle |
| KR20250044256A (ko) | 2022-08-05 | 2025-03-31 | 리제너론 파마슈티칼스 인코포레이티드 | Tdp-43의 응집 저항성 변이체 |
| WO2024064952A1 (en) | 2022-09-23 | 2024-03-28 | Lyell Immunopharma, Inc. | Methods for culturing nr4a-deficient cells overexpressing c-jun |
| WO2024064958A1 (en) | 2022-09-23 | 2024-03-28 | Lyell Immunopharma, Inc. | Methods for culturing nr4a-deficient cells |
| IL319122A (en) | 2022-09-28 | 2025-04-01 | Regeneron Pharma | Different antibody-resistant receptors improve cell-based therapies |
| WO2024077174A1 (en) | 2022-10-05 | 2024-04-11 | Lyell Immunopharma, Inc. | Methods for culturing nr4a-deficient cells |
| JP7353602B1 (ja) | 2022-10-14 | 2023-10-02 | 株式会社インプランタイノベーションズ | ゲノム編集方法およびゲノム編集用組成物 |
| WO2024086805A2 (en) | 2022-10-21 | 2024-04-25 | Watchmaker Genomics, Inc. | Methods and compositions for sequencing library normalization |
| IL320016A (en) | 2022-11-04 | 2025-06-01 | Regeneron Pharma | Calcium voltage-gated channel auxiliary subunit gamma 1 (cacng1) binding proteins and cacng1-mediated delivery to skeletal muscle |
| US20240173426A1 (en) | 2022-11-14 | 2024-05-30 | Regeneron Pharmaceuticals, Inc. | Compositions and methods for fibroblast growth factor receptor 3-mediated delivery to astrocytes |
| JP2025542235A (ja) | 2022-12-22 | 2025-12-25 | キージーン ナムローゼ フェンノートシャップ | プロトプラストカルス接木による再生 |
| IL321908A (en) | 2023-01-27 | 2025-09-01 | Regeneron Pharma | Different rhabdomyovirus glycoproteins and their use |
| CN121127605A (zh) | 2023-03-29 | 2025-12-12 | 阿斯利康(瑞典)有限公司 | 使用抑制剂来提高CRISPR/Cas插入的效率 |
| CN116617414B (zh) * | 2023-03-31 | 2024-04-05 | 中国农业大学 | 一种脂质体及其制备方法和应用 |
| WO2024259309A1 (en) | 2023-06-15 | 2024-12-19 | Regeneron Pharmaceuticals, Inc. | Gene therapy for hearing disorders |
| WO2025006963A1 (en) | 2023-06-30 | 2025-01-02 | Regeneron Pharmaceuticals, Inc. | Methods and compositions for increasing homology-directed repair |
| WO2025029657A2 (en) | 2023-07-28 | 2025-02-06 | Regeneron Pharmaceuticals, Inc. | Anti-tfr:gaa and anti-cd63:gaa insertion for treatment of pompe disease |
| TW202519551A (zh) | 2023-07-28 | 2025-05-16 | 美商雷傑納榮製藥公司 | 用於治療酸性神經髓磷脂酶缺乏症之抗TfR:酸性神經髓磷脂酶 |
| WO2025029654A2 (en) | 2023-07-28 | 2025-02-06 | Regeneron Pharmaceuticals, Inc. | Use of bgh-sv40l tandem polya to enhance transgene expression during unidirectional gene insertion |
| WO2025038750A2 (en) | 2023-08-14 | 2025-02-20 | President And Fellows Of Harvard College | Methods and compositions for treating cancer |
| WO2025049524A1 (en) | 2023-08-28 | 2025-03-06 | Regeneron Pharmaceuticals, Inc. | Cxcr4 antibody-resistant modified receptors |
| WO2025049959A2 (en) | 2023-09-01 | 2025-03-06 | Renagade Therapeutics Management Inc. | Gene editing systems, compositions, and methods for treatment of vexas syndrome |
| WO2025064408A1 (en) | 2023-09-18 | 2025-03-27 | The Broad Institute, Inc. | Compositions and methods for treating cardiovascular disease |
| GB202314578D0 (en) | 2023-09-22 | 2023-11-08 | Univ Manchester | Methods of producing homoplasmic modified plants or parts thereof |
| WO2025081042A1 (en) | 2023-10-12 | 2025-04-17 | Renagade Therapeutics Management Inc. | Nickase-retron template-based precision editing system and methods of use |
| WO2025184567A1 (en) | 2024-03-01 | 2025-09-04 | Regeneron Pharmaceuticals, Inc. | Methods and compositions for re-dosing aav using anti-cd40 antagonistic antibody to suppress host anti-aav antibody response |
| WO2025217398A1 (en) | 2024-04-10 | 2025-10-16 | Lyell Immunopharma, Inc. | Methods for culturing cells with improved culture medium |
| WO2025235388A1 (en) | 2024-05-06 | 2025-11-13 | Regeneron Pharmaceuticals, Inc. | Transgene genomic identification by nuclease-mediated long read sequencing |
| WO2025259669A1 (en) | 2024-06-10 | 2025-12-18 | Regeneron Pharmaceuticals, Inc. | Methods and systems for characterizing modified oligonucleotides |
| WO2025257212A1 (en) | 2024-06-11 | 2025-12-18 | Keygene N.V. | Screening and regeneration of protoplast callus |
| WO2025265017A1 (en) | 2024-06-20 | 2025-12-26 | Regeneron Pharmaceuticals, Inc. | Ass1 gene insertion for the treatment of citrullinemia type i |
| WO2026006542A2 (en) | 2024-06-26 | 2026-01-02 | Yale University | Compositions and methods for crispr/cas9 based reactivation of human angelman syndrome |
Family Cites Families (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3862002A (en) * | 1962-05-08 | 1975-01-21 | Sanfar Lab Inc | Production of physiologically active placental substances |
| DK2325332T3 (da) | 2005-08-26 | 2013-01-28 | Dupont Nutrition Biosci Aps | Anvendelse af CRISPR-associerede gener (CAS) |
| AU2008223544B2 (en) | 2007-03-02 | 2014-06-05 | Dupont Nutrition Biosciences Aps | Cultures with improved phage resistance |
| US20100076057A1 (en) | 2008-09-23 | 2010-03-25 | Northwestern University | TARGET DNA INTERFERENCE WITH crRNA |
| KR101953237B1 (ko) * | 2010-05-17 | 2019-02-28 | 상가모 테라퓨틱스, 인코포레이티드 | 신규 dna 결합 단백질 및 이의 용도 |
| AU2011265733B2 (en) * | 2010-06-14 | 2014-04-17 | Iowa State University Research Foundation, Inc. | Nuclease activity of TAL effector and Foki fusion protein |
| EP2596011B1 (en) * | 2010-07-21 | 2018-10-03 | Sangamo Therapeutics, Inc. | Methods and compositions for modification of a hla locus |
| WO2013141680A1 (en) | 2012-03-20 | 2013-09-26 | Vilnius University | RNA-DIRECTED DNA CLEAVAGE BY THE Cas9-crRNA COMPLEX |
| US9637739B2 (en) | 2012-03-20 | 2017-05-02 | Vilnius University | RNA-directed DNA cleavage by the Cas9-crRNA complex |
| US10266850B2 (en) * | 2012-05-25 | 2019-04-23 | The Regents Of The University Of California | Methods and compositions for RNA-directed target DNA modification and for RNA-directed modulation of transcription |
| CN110669758A (zh) * | 2012-10-23 | 2020-01-10 | 基因工具股份有限公司 | 用于切割靶dna 的组合物及其用途 |
| CA3034794A1 (en) | 2012-12-06 | 2014-06-12 | Sigma-Aldrich Co. Llc | Crispr-based genome modification and regulation |
| EP4234696A3 (en) | 2012-12-12 | 2023-09-06 | The Broad Institute Inc. | Crispr-cas component systems, methods and compositions for sequence manipulation |
| WO2014093701A1 (en) * | 2012-12-12 | 2014-06-19 | The Broad Institute, Inc. | Functional genomics using crispr-cas systems, compositions, methods, knock out libraries and applications thereof |
| RU2721275C2 (ru) | 2012-12-12 | 2020-05-18 | Те Брод Инститьют, Инк. | Доставка, конструирование и оптимизация систем, способов и композиций для манипуляции с последовательностями и применения в терапии |
| CN105121648B (zh) | 2012-12-12 | 2021-05-07 | 布罗德研究所有限公司 | 用于序列操纵的系统、方法和优化的指导组合物的工程化 |
| US8697359B1 (en) | 2012-12-12 | 2014-04-15 | The Broad Institute, Inc. | CRISPR-Cas systems and methods for altering expression of gene products |
| EP2931891B1 (en) * | 2012-12-17 | 2019-05-15 | President and Fellows of Harvard College | Rna-guided human genome engineering |
| AU2014274939B2 (en) * | 2013-06-04 | 2020-03-19 | President And Fellows Of Harvard College | RNA-guideded transcriptional regulation |
| WO2015153647A1 (en) * | 2014-03-31 | 2015-10-08 | Mice With Horns, Llc | Method of preventing or reducing virus transmission in animals |
| EP3359644B1 (en) * | 2015-10-09 | 2024-05-29 | Monsanto Technology LLC | Novel rna-guided nucleases and uses thereof |
| BR112019012155A2 (pt) * | 2016-12-14 | 2019-11-12 | Wageningen Universiteit | uso de pelo menos uma molécula-guia de rna e uma proteína cas, método de ligação, clivagem, marcação ou modificação de um polinucleotídeo alvo de fita dupla, célula transformada, e, complexo de nucleoproteína |
| CN120310773A (zh) * | 2017-11-16 | 2025-07-15 | 阿斯利康(瑞典)有限公司 | 用于改善基于Cas9的敲入策略的有效性的组合物和方法 |
| WO2019217336A2 (en) * | 2018-05-06 | 2019-11-14 | Massachusetts Institute Of Technology | APPLICATIONS OF STREPTOCOCCUS-DERIVED Cas9 NUCLEASSES ON MINIMAL ADENINE-RICH PAM TARGETS |
| EP3821396B1 (en) * | 2018-07-11 | 2022-08-17 | Vilniaus Universitetas | Computer-implemented process on an image of a biological sample |
| US20240279687A1 (en) * | 2021-06-07 | 2024-08-22 | Yale University | Peptide nucleic acids for spatiotemporal control of crispr-cas binding |
| WO2023025767A1 (en) * | 2021-08-23 | 2023-03-02 | Max-Delbrück-Centrum Für Molekulare Medizin In Der Helmholtz-Gemeinschaft | Method for cas9 nickase-mediated gene editing |
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