JP6423338B2 - Cas9−crRNA複合体によるRNA指向性DNA切断 - Google Patents
Cas9−crRNA複合体によるRNA指向性DNA切断 Download PDFInfo
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- JP6423338B2 JP6423338B2 JP2015501880A JP2015501880A JP6423338B2 JP 6423338 B2 JP6423338 B2 JP 6423338B2 JP 2015501880 A JP2015501880 A JP 2015501880A JP 2015501880 A JP2015501880 A JP 2015501880A JP 6423338 B2 JP6423338 B2 JP 6423338B2
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- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- C12N15/90—Stable introduction of foreign DNA into chromosome
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- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- C12N2310/00—Structure or type of the nucleic acid
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/35—Nature of the modification
- C12N2310/351—Conjugate
- C12N2310/3513—Protein; Peptide
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/50—Physical structure
- C12N2310/53—Physical structure partially self-complementary or closed
- C12N2310/531—Stem-loop; Hairpin
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- C12N2320/00—Applications; Uses
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- C12N2800/00—Nucleic acids vectors
- C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
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Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261613373P | 2012-03-20 | 2012-03-20 | |
| US61/613,373 | 2012-03-20 | ||
| US201261625420P | 2012-04-17 | 2012-04-17 | |
| US61/625,420 | 2012-04-17 | ||
| PCT/US2013/033106 WO2013142578A1 (en) | 2012-03-20 | 2013-03-20 | RNA-DIRECTED DNA CLEAVAGE BY THE Cas9-crRNA COMPLEX |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018196574A Division JP7186574B2 (ja) | 2012-03-20 | 2018-10-18 | Cas9-crRNA複合体によるRNA指向性DNA切断 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015510778A JP2015510778A (ja) | 2015-04-13 |
| JP2015510778A5 JP2015510778A5 (enExample) | 2015-10-08 |
| JP6423338B2 true JP6423338B2 (ja) | 2018-11-14 |
Family
ID=48050947
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015501880A Active JP6423338B2 (ja) | 2012-03-20 | 2013-03-20 | Cas9−crRNA複合体によるRNA指向性DNA切断 |
| JP2018196574A Active JP7186574B2 (ja) | 2012-03-20 | 2018-10-18 | Cas9-crRNA複合体によるRNA指向性DNA切断 |
| JP2020174803A Active JP7113877B2 (ja) | 2012-03-20 | 2020-10-16 | Cas9-crRNA複合体によるRNA指向性DNA切断 |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018196574A Active JP7186574B2 (ja) | 2012-03-20 | 2018-10-18 | Cas9-crRNA複合体によるRNA指向性DNA切断 |
| JP2020174803A Active JP7113877B2 (ja) | 2012-03-20 | 2020-10-16 | Cas9-crRNA複合体によるRNA指向性DNA切断 |
Country Status (15)
| Country | Link |
|---|---|
| US (8) | US9637739B2 (enExample) |
| EP (2) | EP3594341A1 (enExample) |
| JP (3) | JP6423338B2 (enExample) |
| CN (2) | CN104520429B (enExample) |
| BR (1) | BR112014023353A2 (enExample) |
| CA (2) | CA3124374C (enExample) |
| DK (1) | DK2828386T3 (enExample) |
| EA (1) | EA029544B1 (enExample) |
| ES (1) | ES2749108T3 (enExample) |
| HU (1) | HUE046295T2 (enExample) |
| IN (1) | IN2014DN07846A (enExample) |
| MX (2) | MX370435B (enExample) |
| PL (1) | PL2828386T3 (enExample) |
| PT (1) | PT2828386T (enExample) |
| WO (1) | WO2013142578A1 (enExample) |
Families Citing this family (434)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103038338B (zh) | 2010-05-10 | 2017-03-08 | 加利福尼亚大学董事会 | 核糖核酸内切酶组合物及其使用方法 |
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| US12533312B2 (en) | 2011-02-04 | 2026-01-27 | Seed Health, Inc. | Method and system for preventing sore throat in humans |
| US11951140B2 (en) | 2011-02-04 | 2024-04-09 | Seed Health, Inc. | Modulation of an individual's gut microbiome to address osteoporosis and bone disease |
| US10583033B2 (en) | 2011-02-04 | 2020-03-10 | Katherine Rose Kovarik | Method and system for reducing the likelihood of a porphyromonas gingivalis infection in a human being |
| US11191665B2 (en) | 2011-02-04 | 2021-12-07 | Joseph E. Kovarik | Method and system for reducing the likelihood of a porphyromonas gingivalis infection in a human being |
| US11523934B2 (en) | 2011-02-04 | 2022-12-13 | Seed Health, Inc. | Method and system to facilitate the growth of desired bacteria in a human's mouth |
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| EP2734621B1 (en) | 2011-07-22 | 2019-09-04 | President and Fellows of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| EP3604555B1 (en) | 2011-10-14 | 2024-12-25 | President and Fellows of Harvard College | Sequencing by structure assembly |
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| GB201122458D0 (en) | 2011-12-30 | 2012-02-08 | Univ Wageningen | Modified cascade ribonucleoproteins and uses thereof |
| US9637739B2 (en) | 2012-03-20 | 2017-05-02 | Vilnius University | RNA-directed DNA cleavage by the Cas9-crRNA complex |
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