JP6423338B2 - Cas9−crRNA複合体によるRNA指向性DNA切断 - Google Patents
Cas9−crRNA複合体によるRNA指向性DNA切断 Download PDFInfo
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- JP6423338B2 JP6423338B2 JP2015501880A JP2015501880A JP6423338B2 JP 6423338 B2 JP6423338 B2 JP 6423338B2 JP 2015501880 A JP2015501880 A JP 2015501880A JP 2015501880 A JP2015501880 A JP 2015501880A JP 6423338 B2 JP6423338 B2 JP 6423338B2
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- C12N15/90—Stable introduction of foreign DNA into chromosome
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- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
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- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- C12N2310/00—Structure or type of the nucleic acid
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- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/35—Nature of the modification
- C12N2310/351—Conjugate
- C12N2310/3513—Protein; Peptide
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/50—Physical structure
- C12N2310/53—Physical structure partially self-complementary or closed
- C12N2310/531—Stem-loop; Hairpin
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- C12N2800/00—Nucleic acids vectors
- C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
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Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261613373P | 2012-03-20 | 2012-03-20 | |
| US61/613,373 | 2012-03-20 | ||
| US201261625420P | 2012-04-17 | 2012-04-17 | |
| US61/625,420 | 2012-04-17 | ||
| PCT/US2013/033106 WO2013142578A1 (en) | 2012-03-20 | 2013-03-20 | RNA-DIRECTED DNA CLEAVAGE BY THE Cas9-crRNA COMPLEX |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018196574A Division JP7186574B2 (ja) | 2012-03-20 | 2018-10-18 | Cas9-crRNA複合体によるRNA指向性DNA切断 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015510778A JP2015510778A (ja) | 2015-04-13 |
| JP2015510778A5 JP2015510778A5 (enExample) | 2015-10-08 |
| JP6423338B2 true JP6423338B2 (ja) | 2018-11-14 |
Family
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Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015501880A Active JP6423338B2 (ja) | 2012-03-20 | 2013-03-20 | Cas9−crRNA複合体によるRNA指向性DNA切断 |
| JP2018196574A Active JP7186574B2 (ja) | 2012-03-20 | 2018-10-18 | Cas9-crRNA複合体によるRNA指向性DNA切断 |
| JP2020174803A Active JP7113877B2 (ja) | 2012-03-20 | 2020-10-16 | Cas9-crRNA複合体によるRNA指向性DNA切断 |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018196574A Active JP7186574B2 (ja) | 2012-03-20 | 2018-10-18 | Cas9-crRNA複合体によるRNA指向性DNA切断 |
| JP2020174803A Active JP7113877B2 (ja) | 2012-03-20 | 2020-10-16 | Cas9-crRNA複合体によるRNA指向性DNA切断 |
Country Status (15)
| Country | Link |
|---|---|
| US (8) | US9637739B2 (enExample) |
| EP (2) | EP3594341A1 (enExample) |
| JP (3) | JP6423338B2 (enExample) |
| CN (2) | CN114410625B (enExample) |
| BR (1) | BR112014023353A2 (enExample) |
| CA (2) | CA2867849C (enExample) |
| DK (1) | DK2828386T3 (enExample) |
| EA (1) | EA029544B1 (enExample) |
| ES (1) | ES2749108T3 (enExample) |
| HU (1) | HUE046295T2 (enExample) |
| IN (1) | IN2014DN07846A (enExample) |
| MX (2) | MX370435B (enExample) |
| PL (1) | PL2828386T3 (enExample) |
| PT (1) | PT2828386T (enExample) |
| WO (1) | WO2013142578A1 (enExample) |
Families Citing this family (437)
| Publication number | Priority date | Publication date | Assignee | Title |
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| BR112012028805A2 (pt) | 2010-05-10 | 2019-09-24 | The Regents Of The Univ Of California E Nereus Pharmaceuticals Inc | composições de endorribonuclease e métodos de uso das mesmas. |
| US10687975B2 (en) | 2011-02-04 | 2020-06-23 | Joseph E. Kovarik | Method and system to facilitate the growth of desired bacteria in a human's mouth |
| US11951139B2 (en) | 2015-11-30 | 2024-04-09 | Seed Health, Inc. | Method and system for reducing the likelihood of osteoporosis |
| US12279989B2 (en) | 2011-02-04 | 2025-04-22 | Seed Health, Inc. | Method and system for increasing beneficial bacteria and decreasing pathogenic bacteria in the oral cavity |
| US12533312B2 (en) | 2011-02-04 | 2026-01-27 | Seed Health, Inc. | Method and system for preventing sore throat in humans |
| US11523934B2 (en) | 2011-02-04 | 2022-12-13 | Seed Health, Inc. | Method and system to facilitate the growth of desired bacteria in a human's mouth |
| US11844720B2 (en) | 2011-02-04 | 2023-12-19 | Seed Health, Inc. | Method and system to reduce the likelihood of dental caries and halitosis |
| US11951140B2 (en) | 2011-02-04 | 2024-04-09 | Seed Health, Inc. | Modulation of an individual's gut microbiome to address osteoporosis and bone disease |
| US12257272B2 (en) | 2015-12-24 | 2025-03-25 | Seed Health, Inc. | Method and system for reducing the likelihood of developing depression in an individual |
| US10583033B2 (en) | 2011-02-04 | 2020-03-10 | Katherine Rose Kovarik | Method and system for reducing the likelihood of a porphyromonas gingivalis infection in a human being |
| US11191665B2 (en) | 2011-02-04 | 2021-12-07 | Joseph E. Kovarik | Method and system for reducing the likelihood of a porphyromonas gingivalis infection in a human being |
| US11998479B2 (en) | 2011-02-04 | 2024-06-04 | Seed Health, Inc. | Method and system for addressing adverse effects on the oral microbiome and restoring gingival health caused by sodium lauryl sulphate exposure |
| BR112013024337A2 (pt) | 2011-03-23 | 2017-09-26 | Du Pont | locus de traço transgênico complexo em uma planta, planta ou semente, método para produzir em uma planta um locus de traço transgênico complexo e construto de expressão |
| EP2734621B1 (en) | 2011-07-22 | 2019-09-04 | President and Fellows of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| EP3604555B1 (en) | 2011-10-14 | 2024-12-25 | President and Fellows of Harvard College | Sequencing by structure assembly |
| WO2014163886A1 (en) | 2013-03-12 | 2014-10-09 | President And Fellows Of Harvard College | Method of generating a three-dimensional nucleic acid containing matrix |
| ES2991004T3 (es) | 2011-12-22 | 2024-12-02 | Harvard College | Métodos para la detección de analitos |
| GB201122458D0 (en) | 2011-12-30 | 2012-02-08 | Univ Wageningen | Modified cascade ribonucleoproteins and uses thereof |
| US9637739B2 (en) | 2012-03-20 | 2017-05-02 | Vilnius University | RNA-directed DNA cleavage by the Cas9-crRNA complex |
| CN104364380B (zh) | 2012-04-25 | 2018-10-09 | 瑞泽恩制药公司 | 核酸酶介导的使用大靶向载体的靶向 |
| PL4289948T3 (pl) * | 2012-05-25 | 2025-06-02 | The Regents Of The University Of California | Sposoby i kompozycje do modyfikacji kierowanego na rna docelowego dna i do nakierowanej na rna modulacji transkrypcji |
| KR20230065381A (ko) * | 2012-07-25 | 2023-05-11 | 더 브로드 인스티튜트, 인코퍼레이티드 | 유도 dna 결합 단백질 및 게놈 교란 도구 및 이의 적용 |
| EP2912175B1 (en) | 2012-10-23 | 2018-08-22 | Toolgen Incorporated | Composition for cleaving a target dna comprising a guide rna specific for the target dna and cas protein-encoding nucleic acid or cas protein, and use thereof |
| CN105142669B (zh) | 2012-12-06 | 2018-07-03 | 西格马-奥尔德里奇有限责任公司 | 基于crispr的基因组修饰和调控 |
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| WO2014093655A2 (en) * | 2012-12-12 | 2014-06-19 | The Broad Institute, Inc. | Engineering and optimization of systems, methods and compositions for sequence manipulation with functional domains |
| PT2898075E (pt) | 2012-12-12 | 2016-06-16 | Harvard College | Manipulação e otimização de sistemas, métodos e composições de enzima melhorados para manipulação de sequências |
| CN105121648B (zh) | 2012-12-12 | 2021-05-07 | 布罗德研究所有限公司 | 用于序列操纵的系统、方法和优化的指导组合物的工程化 |
| CN105658796B (zh) | 2012-12-12 | 2021-10-26 | 布罗德研究所有限公司 | 用于序列操纵的crispr-cas组分系统、方法以及组合物 |
| WO2014093622A2 (en) | 2012-12-12 | 2014-06-19 | The Broad Institute, Inc. | Delivery, engineering and optimization of systems, methods and compositions for sequence manipulation and therapeutic applications |
| EP4282970A3 (en) * | 2012-12-17 | 2024-01-17 | President and Fellows of Harvard College | Rna-guided human genome engineering |
| AU2014207618A1 (en) | 2013-01-16 | 2015-08-06 | Emory University | Cas9-nucleic acid complexes and uses related thereto |
| KR20170108172A (ko) | 2013-03-14 | 2017-09-26 | 카리부 바이오사이언시스 인코포레이티드 | 핵산-표적화 핵산의 조성물 및 방법 |
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| US9340800B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | Extended DNA-sensing GRNAS |
| US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
| US9526784B2 (en) * | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
| EP3418379B1 (en) | 2013-09-18 | 2020-12-09 | Kymab Limited | Methods, cells & organisms |
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