MX370435B - CORTE DE ADN DIRIGIDO POR ARN, POR MEDIO DEL COMPLEJO Cas9-crRNA. - Google Patents

CORTE DE ADN DIRIGIDO POR ARN, POR MEDIO DEL COMPLEJO Cas9-crRNA.

Info

Publication number
MX370435B
MX370435B MX2014011279A MX2014011279A MX370435B MX 370435 B MX370435 B MX 370435B MX 2014011279 A MX2014011279 A MX 2014011279A MX 2014011279 A MX2014011279 A MX 2014011279A MX 370435 B MX370435 B MX 370435B
Authority
MX
Mexico
Prior art keywords
cas9
vitro
rna
polypeptide
crrna
Prior art date
Application number
MX2014011279A
Other languages
English (en)
Other versions
MX2014011279A (es
Inventor
Smith Anja
Siksnys Virginijus
Gasiunas Giedrius
Karvelis Tautvydas
Lubys Arvydas
Zaliauskiene Lolita
Glemzaite Monika
Original Assignee
Univ Vilnius
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=48050947&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX370435(B) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Univ Vilnius filed Critical Univ Vilnius
Publication of MX2014011279A publication Critical patent/MX2014011279A/es
Publication of MX370435B publication Critical patent/MX370435B/es

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    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3513—Protein; Peptide
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/531—Stem-loop; Hairpin
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    • C12N2320/00—Applications; Uses
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La presente invención se refiere a métodos in vitro para la modificación específica de sitio de una molécula de ADN objetivo. Dichos métodos pueden incluir ensamblar un complejo de Cas9-crRNA recombinante in vitro combinando una proteína Cas9, un crRNA diseñado, y un tracrRNA en condiciones adecuadas para la formación del complejo, y poner en contacto una molécula de ADN objetivo con el complejo de Cas9-crRNA recombinante. El crRNA diseñado puede ser capaz de dirigirse de forma universal y programarse para guiar el complejo Cas9-crRNa recombinante a una región que comprende un sitio en la molécula de DNA objetivo, y la secuencia de crRNA puede ser reprogramada para que sea heteróloga a la proteína Cas9. La modificación específica de sito de lo molécula de ADN objetivo es un corte de la molécula de ADN objetivo.
MX2014011279A 2012-03-20 2013-03-20 CORTE DE ADN DIRIGIDO POR ARN, POR MEDIO DEL COMPLEJO Cas9-crRNA. MX370435B (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261613373P 2012-03-20 2012-03-20
US201261625420P 2012-04-17 2012-04-17
PCT/US2013/033106 WO2013142578A1 (en) 2012-03-20 2013-03-20 RNA-DIRECTED DNA CLEAVAGE BY THE Cas9-crRNA COMPLEX

Publications (2)

Publication Number Publication Date
MX2014011279A MX2014011279A (es) 2015-05-11
MX370435B true MX370435B (es) 2019-12-11

Family

ID=48050947

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2014011279A MX370435B (es) 2012-03-20 2013-03-20 CORTE DE ADN DIRIGIDO POR ARN, POR MEDIO DEL COMPLEJO Cas9-crRNA.
MX2019009736A MX2019009736A (es) 2012-03-20 2014-09-19 Corte de adn dirigido por arn, por medio del complejo cas9-crrna.

Family Applications After (1)

Application Number Title Priority Date Filing Date
MX2019009736A MX2019009736A (es) 2012-03-20 2014-09-19 Corte de adn dirigido por arn, por medio del complejo cas9-crrna.

Country Status (15)

Country Link
US (8) US9637739B2 (es)
EP (2) EP2828386B1 (es)
JP (3) JP6423338B2 (es)
CN (2) CN104520429B (es)
BR (1) BR112014023353A2 (es)
CA (2) CA3124374C (es)
DK (1) DK2828386T3 (es)
EA (1) EA029544B1 (es)
ES (1) ES2749108T3 (es)
HU (1) HUE046295T2 (es)
IN (1) IN2014DN07846A (es)
MX (2) MX370435B (es)
PL (1) PL2828386T3 (es)
PT (1) PT2828386T (es)
WO (1) WO2013142578A1 (es)

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