CN1140159A - Method for producing organic and inorganic mixed microbe fertilizer - Google Patents

Abstract

An organic-inorganic compound microbe fertilizer is made from natural, industrial and agriculture waste through partial degradation to become C and N sources, propagation of yeast and useful fungus as organic nitrogen nutrient, multiplication of Aspergillus niger to activate bound P in lattice state in phosphorus ore into quick-acting P, and breeding silicate bacteria to activate minerals (potash feldspar and alunite powder) containing potassium oxide in lattice state into quick-acting K.

Description

A kind of new microbial composite organic-inorganic fertilizer manufacture method

The present invention relates to composite bio-fertilizer, specifically be meant a kind of new microbial composite organic-inorganic fertilizer manufacture method.

Owing to use chemical fertilizer for a long time, merely, soil microorganisms quantity is sharply reduced, soil organic matter content descends, and the soil water-reataining fertilizer-keeping ability reduces thereupon, causes soil hardening.Farm crop are owing to draw a large amount of chemical fertilizer, make resistance against disadvantage more and more a little less than, cause disease and pest more and more serious, the chemical pesticide amount of application is more and more, make the chemical residual amount of agricultural-food more and more higher, the quality of agricultural-food causes the agroecosystem vicious cycle worse and worse.Because applying quantity of chemical fertilizer is increasing, farm crop only reach 20%-30% to the utilization ratio of chemical fertilizer, and a large amount of chemical fertilizer are by leaching, polluted underground water or by the face of land through the inflow rivers,lakes and seas, environment has been caused severe contamination, destroyed the natural eubiosis.

Because each side factor, chemical fertilizers production suffers great loss, China's year needs 1.2 hundred million tons of chemical fertilizer, homemade more than 9,000 ten thousand tons, more than 3,000 ten thousand tons of imports, because chemical fertilizer industry is had difficulty in taking a step, price rises steadily, and causes the agricultural burden more and more heavier, and input-output ratio is more and more littler, if add the generalized grain amount of increase, the people's the standard of living and the every aspect of national economy will be had a strong impact on.

In order to overcome all drawbacks of for a long time using chemical fertilizer merely and being caused, set up Organic farming alliance in the world, encourage not to fertilize, produce organic farm products.Japan also is that oil-agriculture changes to Organic farming with inorganic agriculture, the method for having existed side by side.China has proposed " two high one is excellent " agricultural and " sustainable development " agricultural.In sum, aspect fertilizer, realize above-mentioned transformation, all require applying organic manure, reduce fertilizer amount or chemical fertilizer is carried out bio-transformation making it innoxious and the increase chemical fertilizer utilization ratio.Make fertilizer give full play to fertilizer efficiency, must give up the custom that falls behind of traditional pile fermentation.The organic matter materialization of going ahead of the rest handle is kept the activation nutrient, the degraded poisonous substance, eliminate harmful bacterium, increase and transform nutrition in the organic matter with beneficial microorganism, make it to satisfy the needs of farm crop to nutrition, conversion to inorganic mineral nutrition, be not to use chemical process, invalid attitude becomes available state in the microbial process conversion mineral but utilize, and Here it is will pass by merely based on chemical fertilizer, be transformed into based on the organic mineral behind the microbial process, chemical fertilizer is the policy of assisting, and the chemical fertilizer of adding is increased operation rate, and makes soil obtain improvement, agricultural product quality is improved, the farm crop resistance against disadvantage increases, and has reduced the disease generation, Shaoshi chemical pesticide or do not execute chemical pesticide.Because fertilizer amount significantly reduces, and has improved utilization ratio, has avoided the chemical residual in the agricultural-food, utilized agriculture and industry waste again, make the energy and resource all obtain abundant reasonable use, environment is improved, the ecosystem is circulated benignly.

Purpose of the present invention just is the agricultural that realizes " two high one is excellent " for real, and " ecological agriculture ", " sustainable agriculture " propose a kind of manufacture method of new microbial composite organic-inorganic fertilizer.

The manufacture method of microbial organic/inorganic compound fertilizer of the present invention comprises:

Utilize nature and agriculture and industry waste to be raw material, adopt physics, chemical process, make it part and be degraded to monose, disaccharide, trisaccharide and tetrose as carbon source, protein degradation be peptide class and amino acid as nitrogenous source, also can add small amounts of inorganic nitrogen nitrogenous source as a supplement;

Utilize above-mentioned carbon, nitrogenous source, breeding yeast and useful fungi produce a large amount of single cell proteins, or mycelium protein, make it to become the organic nitrogen nutrition in the compound manure; The genus bacillus that adds the energy decomposing protein then makes it to resolve into gradually quick-acting nitrogen;

Utilize above-mentioned carbon, nitrogenous source breeding black-koji mould, making it at pH value is to produce oxalic acid under the neutral condition, constraint phosphorus with part crystal lattice state in the activating phosphorite powder, become rapid available phosphorus, make it to become the plain nutrition of phosphorus in the compound manure, the phosphate use inorganic phosphorus bacteria that is fixed in the constraint phosphorus of the crystal lattice state that all the other are not activated and the soil continues activation and make it to continue to increase rapid available phosphorus in soil;

Utilize above-mentioned carbon source and gaseous nitrogen breeding silicate bacteria, make it to activate the mineral substance that contains lattice attitude potassium oxide such as pulvis such as potassium felspar sand, alunite, making it part becomes available potassium; The potassium that all the other ends are activated partly and soil is fixed is added silicate bacteria before granulation or other has the microorganism of ability of dissolving potassium to make it to continue to increase effective potassium;

Based on above nitrogen, phosphorus, potassium nutrition and microbial metabolites enzyme and the remaining organic matter that forms through microbial process, with the nitrogen of inorganic fertilizer, phosphorus, potassium and an amount of middle amount, micro-rare elements, rare earth element is adjusted mutually and replenish, and forms that nutrition is complete, validity is high, with low cost, free from environmental pollution, the microbial organic/inorganic compound fertilizer that can improve the soil, improve quality of agricultural product.

The applied bacterial classification of the present invention is respectively: classification Chinese name Latin literary fame is preserved and number to be preserved 15 days November nineteen ninety-five of day (budding fungus) (rhodothece rubra) Rhodotorula NO0244

15 days November nineteen ninety-five of rubra (fungi Aspergillaceae) (aspergillus niger) Aspergillus NO0244

15 days November nineteen ninety-five of niger (bacterial spore Bacteriaceae) (subtilis) Bacillus NO0244

15 days November nineteen ninety-five of subtilis (bacterial spore Bacteriaceae) (bacillus megaterium) Bacillus NO0244

15 days November nineteen ninety-five of megatherium (bacterial spore Bacteriaceae) (bacillusmusilaginosiengineering) Bacillus NO0244

15 days November nineteen ninety-five of mucilaginosus (budding fungus) (candida tropicalis) Candida NO0243

15 days November nineteen ninety-five of tropicalis (bacterium class lactobacillaceae) (Lactobacterium acidophilum) Lactobacillus NO0243

acidophilus

Raw material of the present invention is extremely extensive, the organic substance raw material is as being rich in xylogen, and the crop tangerine bar of Mierocrystalline cellulose, hemicellulose, pectin substance is as corn tangerine, soybean tangerine; Cotton tangerine grass class is as straw, wheat straw, fodder grasses, weeds; The slag class has: beet pulp, bagasse; The cot class is as: rice husk, Exocarpium cocois (Cocos nucifera L), tung oil shell, Pericarppium arachidis hypogaeae, last of the ten Heavenly stems flower seed shell; Wood machining residues is as sawdust, wood shavings, leaf; Be rich in peat composed of rotten mosses class, the peat class of humic acid; Brown coal, weathered coal class, the city organic matter rubbish class after the letter sorting; The Xylitol slag that Xylitol factory produces; The furfural dregs that furfural factory produces; Rich in starch and proteinic slag class such as bean dregs, vermicelli slag, fecula; The remaining puffed soybean in oil expression back is as dregs of beans, sunflower seed dregs, the Semen Brassicae campestris dregs of rice, peanut meal, cottonseed meal, castor seed meal, Semen Ricini, the tung oil dregs of rice; Industrial fermentation waste material melt cinder, poor class, the scrap leather angle of the animal blood of, residue of monosodium glutamate poor as beer slag, distillers ' grains, alcohol, acid-sludge, residue of soya, ferment glycerin slag, fermentation solvent slag, citric acid fermented slag, the amino acid sludge of fermentation, high fructose syrup screenings meat connection source mill, hair, skin, bone, internal organ, leather processing factory, bits etc. reach people, animal, poultry manure.

Described mineral raw material, phosphorus ore containing comprises: phosphatic rock, phosphorite, potassium-bearing mineral, potassium felspar sand, alunite, mica; The micro-mineral of amount comprise rhombspar, Wingdale, lime dinas, the tremolite, diopside, magnesium borate ore, Tremoliteor Tremoliteasbestos, serpentine, calcite, aragonite, giobertite in containing; Contain heavy metal face below the intermediate value, iron, manganese, copper, zinc, molybdenum, boron rock, zeolite ore deposit, contain the medical stone ore deposit of yttrium, contain the mishmetal of rare earth element, rare earth nitrate or contain useless smelting ore deposit quarrel of rare earth element etc.; Above raw material is worn into powder.

Among the present invention, make Mierocrystalline cellulose, hemicellulose, pectin substance, starchiness class in the organic matter material, under acidic conditions, under 100 ℃～400 ℃ temperature condition, under the pressure condition of 0.1MPa～3.0MPa, make it part generation monose and disaccharide, trisaccharide, tetrose; Make the protein transduction in the organic matter become peptide class and amino acid; For the low organic matter of protein content, use inorganic nitrogen, be to provide a large amount of carbon source and nitrogenous sources as additional purposes such as carbon ammonium, urea, sulphur ammoniums for microbial reproduction.

Utilize above-mentioned carbon source and nitrogenous source among the present invention, breeding yeast or useful fungi such as aspergillus mould (according to a conventional method).Accumulation single cell protein, or mycelium protein.With this as the organic nitrogen nutrition in the composite fertilizer.Also the inorganic nitrogen in the chemical fertilizer can be changed into protein attitude nitrogen, make it innoxious.Before granulation, add to produce the subtilis of proteolytic enzyme, make it in soil, to resolve into polypeptide, amino acid, until ammonia.For farm crop provide quick-acting nitrogen; Wherein the amount of the subtilis of Jia Ruing is the bacterium liquid (20～10,000,000,000/ml) of 1 ‰～10% (weight percent) of adding carrier amount.

Among the present invention, the generation of rapid available phosphorus is the oxalic acid activating phosphorite powder that the black-koji mould with 1 ‰～10% (weight percent) of ground phosphate rock produces under the pH value neutrallty condition, makes it part and changes into rapid available phosphorus.The particle diameter of required ground phosphate rock need reach 80～250 orders.Remaining invalid phosphorus before granulation, adds phosphate-solubilizing bacteria again, as adds 1 ‰～10% bacillus megaterium, makes it to continue in soil phosphorus decomposing.

Among the present invention, the generation of available potassium is to utilize silicate bacteria (or other has the microorganism of ability of dissolving potassium) to carry out potassium decomposing, as 1%～10% bacillusmusilaginosiengineering with feldspar in powder.Remaining invalid potassium is added 1 ‰～10% silicate bacteria of feldspar in powder again before granulation, make it in soil, continues potassium decomposing, and silicate bacteria can utilize organic carbon to make carbon source, but can not utilize organonitrogen, needs do nitrogenous source with gaseous nitrogen.Potassium decomposing simultaneously, contained moderate-element also is activated simultaneously as silicon, calcium, magnesium, sulphur and micro-rare elements, rare earth element, or amount, trace element, rare elements in adding simultaneously, the rare earth element breeze makes it to activate simultaneously also is feasible.

According to Different Crop, Ca, Mg, S, Si trace element Fe, Mn, Co, Zn, Mo, B5～10%, rare elements 1 ‰～10%, rare earth element 1 ‰～10% (weight percent) with an amount of inorganic nitrogen, phosphorus, potash fertilizer 5%～60% (weight percent) and middle amount, 10%～36% (weight percent) replenish, satisfy crop in whole growing to the available nutrient demand, it is long-lasting that composite fertilizer had both been had, has quick-acting again, both free from environmental pollution, improved quality product again, microorganism, organic and inorganic composition are played a role separately.

Among the present invention, contain the organic mineral part and the organic-inorganic chemical fertilizer part that does not contain microorganism of microorganism, granulation respectively mixes between particle then.Make chemical fertilizer not contact microorganism fully, make microorganism keep original activity, and be not subjected to the influence of chemical fertilizer.With fine perlite powder, expanded ground silica or expanded careless class powder absorption, wherein, proportioning is 0.1～5% microorganism: 20～60% organic matter: the inanimate matter of surplus, this can do corresponding adjustment according to different farm crop to the microorganism that the back adds earlier.

Among the present invention, operational path is more flexible, and system 1,2,3 can independently constitute a goods batch, and also homologous ray 4 constitutes a goods batch separately, also can cooperate to each other to constitute a goods batch.

Among the present invention, each system adds before granulation and helps the granulating additive.Its composition has mineral substance and tackiness agent such as wilkinite, and its add-on is 1%～10% of a total amount.

New microbial provided by the present invention, organic and inorganic composite fertilizer have broken the mindset of traditional bacterial manure, have overcome the limitation of present microbial fertilizer.As required, increased new bacterial strain, the organic carbon that can not or be difficult to be utilized by microorganism with physico-chemical process changes into monose or disaccharide, trisaccharide, tetrose fast, desirable carbon source as the quick breeding of microorganism, accomplished the meta-bolites of thorough sterilization and the assorted bacterium of degraded simultaneously, protein is converted into amino acid fast or adds the desirable nitrogenous source of inorganic nitrogen to breed fast as microorganism.

Utilize the formed carbon source of above technology, nitrogenous source, breeding yeast or useful fungi such as aspergillus tubigensis form a large amount of single cell protein or mycelium protein, breed milk-acid bacteria simultaneously to suppress the growth of assorted bacterium.This just on the trophic level of original raw material, has improved organic nitrogen trophic level greatly, but also inorganic nitrogen has been carried out bio-transformation, forms innoxious organonitrogen, is made for the nitrogen nutrition that thing needs.In order to increase the quick-acting of organic nitrogen nutrition, before the granulation, add the bacillus that produces proteolytic enzyme, make it in soil, protein transduction be turned to amino acid so that ammonia directly absorbs in order to crop.Add the organophosphorus decomposer simultaneously, the organophosphorus in the protein is changed into inorganic phosphorus, be made for the absorption of thing rapid available phosphorus.

Raw materials used for the plain nutrition of phosphorus in the micro organism composite fertilizer, directly utilize phosphorus ore containing such as phosphatic rock, phosphorite etc.In process of production, utilize above-mentioned carbon, nitrogenous source breeding aspergillus niger makes deactivate lattice constraint phosphorus in the ground phosphate rock of secreted oxalic acid, becomes the part rapid available phosphorus.By oven dry to material, knocked out black-koji mould after, before granulation, add the bacillus of phosphorus decomposing, make it in soil, to continue phosphorus decomposing.

For the potassium nutrition in the micro organism composite fertilizer, raw materials used, for china natural resources is abundant, widely distributed low grade potassium-containing mineral such as potassium felspar sand, alunite, mica etc.Utilize above-mentioned carbon source and gaseous nitrogen, silicate bacteria is bred in a large number, make lattice attitude potassium partly change into available potassium, middle amount wherein and trace element, rare elements, rare earth element have also obtained activation simultaneously.Before granulation, add silicon salt bacterium again, make it in soil, to continue activation lattice attitude potassium.

For satisfy crop in whole growing to quick-acting nutritious needs, need add chemical fertilizer, an additional way part is added in the microorganism organic moiety, dosage is killed for not making microorganism, all the other chemical fertilizer are organic with part, and moderate-element, trace element, rare elements, rare earth element be granulation separately together, and then with after the fertilizer each component granulation after above-mentioned each microbial transformation, row mixes again.

The novel microorganism organic-inorganic compound fertilizer is based on the microorganism organic mineral.Chemical fertilizer is the reasonable structure form of assisting.

The present invention also relates to a kind of amount, micro-, rare, rare-earth mineral of utilizing in nature and industrial or agricultural and organic garbage of city waste and the phosphorous potassium directly with the microorganisms and the conversion farm crop desired nutritional of multiple function, be aided with an amount of chemical fertilizer again, make a kind of new processing method of new microbial composite organic-inorganic fertilizer.

As follows below in conjunction with drawings and Examples to the detailed description of the invention:

Fig. 1 is a process flow sheet of the present invention.

Embodiment 1

Organic waste I is carried out impurity elimination, select purification, first broken, become machinable material 2 and be transported in the activation degraded jar 5, pressure is 0.1-3MPa, is fed in raw material in 5 by acid storage tank 4a, and dosage is the 1%-5% of material.It is 80 ℃～300 ℃ by thermal source heating (heat-conducting oil heating after available the heating, also available vapour heating) Heating temperature then.The activation degradation time is 20 minutes-240 minutes.Then, add in ammoniacal liquor or the water-soluble carbon ammonium and PH5-7 and control suitable moisture content, enter in the storage tank 6, supply with three systems then by storage alkali jar 4b.

In the system 1, in cultured yeast or aspergillus tubigensis jar 1-1 (culturing process slightly), add Lactobacterium acidophilum simultaneously suppressing assorted bacterium, yeast or aspergillus tubigensis liquid are joined in the fermentor tank or pond 1-2 that is equipped with from carbon, nitrogenous source in 6, carry out yeast aspergillus propagation enlarged culturing.Time is 24-96 hour, and temperature is 28 ℃～32 ℃, adds other nutritive substance and carries out the routine cultivation.After single cell protein accumulation maximum, enter among the oven drying at low temperature machine 1-3, carry out the low temperature dewatering oven dry.Temperature is no more than 80 ℃ of enzyme deactivations that do not make generation.Enter then among the stirrer 1-4, add Bacillus subtillis and the Bacillus megatherium dosage is respectively 1 ‰～10% of material, add before earlier with a spot of expanded inorganic substance, mix with flora as expanded silica, fine perlite, make flora by absorption to inanimate matter, and then mix with organism, make that the proteolytic enzyme of its generation decomposes organonitrogen with Phospholipid hydrolase, phosphorus efficiency phosphorus becomes amino acid and inorganic phosphorus.For crop provides quick-acting nitrogen, phosphorus nutrition.After treating fully to stir, enter among the tablets press 1-5 after the granulation, enter cooler 1-5a again, enter hopper 1-6 then,, enter in the mixer 7 through the belt weigher.

System 2: enter among fermentor tank or the fermentation vat 2-2 from basin 6 a part of materials, by aspergillus niger culture systems 2-1 (concrete culturing process slightly) inoculated aspergillus niger with add other nutritive salt, turned sour time 1-7 days.Culture temperature 28-32 ℃, the oxalic acid that makes it to produce forms caoxalate with the reaction of the calcium in the ground phosphate rock, makes phosphoric acid partly activate out, forms rapid available phosphorus.After this process is finished, enter among the biologic phosphorus fertilizer basin 2-3, then, enter among the dryer 2-4, carry out sterilizing, drying.Enter again and mix among the stirred pot 2-6; supply with an amount of bacterium liquid by cultured silicate bacteria seeding tank 2-5, dosage is 1 ‰～10%, adds before earlier with a spot of expanded inorganic substance; mix with flora as expanded silica, fine perlite or expanded careless class; make flora by absorption on inanimate matter or organic matter, and then and mixing of materials, after mixing; enter among the tablets press 2-7; and then enter among the cooler 2-7a, after the metering, enter and always puddle in the device 7 among the last hopper weighing belt 2-8.

System 3: the material by supplying with in 6, enter among fermentor tank or the pond 3-2, supply with bacterium liquid or solid fungicide by silicate bacteria seeding tank or groove 3-1.The air of the air of being supplied with for purifying carries out growing microorganism through supplying with to gaseous nitrogen source.Lattice attitude in the potassium felspar sand is changed into effective potassium of part.Leavening temperature is 28～30 ℃, and incubation time is 24-168 hour, enters then among basin or the storage pond 3-3, enters temperature sterilization among the oven drying at low temperature machine 3-4, bake out temperature 40-80 ℃ again, enters then among the steel basin 3-5.The silicate bacteria that adds material 1 ‰～10% again from 3-1 becomes available potassium so that continue to decompose activation lattice potassium in soil; Earlier with a spot of expanded inorganic substance, mixes with flora before adding as expanded silica, fine perlite or the careless class of expanded organic matter, make flora by absorption to inanimate matter, and then mix with organism.Then, after tablets press 2-6 granulation, enter again in the 2-6a cooler, enter then among the hopper 2-7 weighing belt after metering, enter in total mixer 7, puddle.

System 4: organic and inorganic nitrogenous fertilizer basin 4-1 from part; organic and the inorganic phosphorus fertilizer basin 4-2 of part; organic and the inorganic potash fertilizer basin 4-3 of part, part organic matter and moderate-element, trace element, rare elements, rare earth element 4-4-1 are mixed by said ratio and applied farm crop; enter among the stirrer 4-4; after tablets press 4-5 granulation, enter in the 4-5a cooler, and then in stirring hopper weighing belt 4-6; after metering, enter in the mixer 7.After mixer 7 is evenly puddled, after quality inspection 8 quality inspections are qualified, advance metering packing scale 9 finished product, put 10 at last in storage.

The toxicological experiment result of composite fertilizer provided by the present invention is:

Composite fertilizer's rat teratogenic test

For detecting the teratogenesis of composite fertilizer to rat.By " teratogenic test " method in " toxicity of pesticide test method temporary provisions ", test.

Sample: Changchun Chaoyang District institute of microbiology of science and technology industry group provides; Dark gray powder, tasteless.

Animal: by the healthy adult Wistar kind big white mouse that institute for drug control, Jilin Province animal housing provides, male 50, female 120, the healthy observation after three days experimentizes.

Contamination; By the requirement of censorship unit, establish three experimental group, be respectively acute toxicity test LD ₅₀Value 1/4,1/15,1/50, promptly 3,75g/Kg body weight, 1g/Kg body weight, 0.3g/Kg body weight.Other establishes solvent (vegetables oil) control group, with the vegetables oil heated and boiled, makes up a prescription after the cooling when experimental group is made up a prescription, and refrigerator is preserved.Dosage 1ml/100g body weight, the male and female mouse is by same cage at 1: 1 night.The female mouse that to find cloudy bolt inferior morning is decided to be pregnant 0 day, random packet, and each treated animal is all from 6-15 days, and be administered once every day.And weighed at pregnant 0,5,10,20 day.Put to death animal in back 20 days in becoming pregnant, cut open the belly and cut the uterus and get the tire son, check, make sample by " program " requirement.

Test-results

Table 1 composite fertilizer influences dosage female female total conceptual quotient (g/Kg. body weight) mouse of not becoming pregnant of becoming pregnant to the female mouse conceptual quotient of each group fertilization and counts mouse several 3.75 13 9 22 59.1 1.0 13 16 29 44.8 0.3 12 15 27 44.4

The continuous page table 0 12 16 28 42.9 of going up of table continued on next page adds up to 50 56 106

Relatively there are no significant the difference (P＞0.05) of each experimental group and control group shown in the last table, promptly composite fertilizer does not have obvious influence to conceptual quotient.

Table 2 composite fertilizer checks several 14 ribs 3.75 120 0 45 0 75 0 1.0 127 0 51 0 76 0 0.3 120 0 43 0 77 10 143 0 49 0 94 0 of the routine number deformity of skeletal survey (g/Kg. body weight) numerical example number deformity numerical example to tire son's the dosage visual examination internal organ that affect

Shown in the last table, do not find outward appearance, internal organ deformity, have the bone inspection to find that 0.3g/Kg body weight group has the tire son of one 14 rib, learn by statistics and handle each experimental group and control group comparison there are no significant difference (P＞0.05).

Table 3 composite fertilizer affects relatively P＜0.05 of the routine number X ± SD of the routine number X ± SD of dosage height weight fontanel (g/Kg body weight) routine number X ± SD (cm) (g) (cm) 3.75 120 3.50 ± 0.38 120 3.60 ± 0.86**, 120 0.29 ± 0.06**, 1.0 127 3.58 ± 0.44* 127 3.90 ± 0.54** 127 0.27 ± 0.04 0.3 120 3.60 ± 0.38*, 120 3.35 ± 0.75** 120 0.27 ± 0.04 0 143 3.42 ± 0.65 143 3.10 ± 1.05 143 0.26 ± 0.06 notes: * and control group, * * and control group comparison P＜0.01 to what the tire son grew

Relatively there were significant differences (P＜0.01) through comparing body weight each experimental group of index and control group; In the height index, in, low dose group and control group have notable difference (P＜0.01).

The Salmonella reversion test result

Concentration (mg/ml)	TA 102?-S9 +S9	TA 102?-S9 +S9	TA 102?-S9 +S9	TA 102?-S9 +S9
					200 20 2	?239 249 ?217 247 ?220 263	?96 232 ?95 204 ?88 185	?24 40 ?25 38 ?24 41	?102 177 ?132 178 ?108 184
Positive control is from beaming back parameter	?1098 916 226	?497 248 166	?509 248 33	?731 585 126

The result finds out from the Ames experiment statistics: composite fertilizer is to four kinds of bacterial strains of Salmonella typhimurium TA series, by 2-200mg/ml, whether no matter each concentration add S9, and every ware returns the change colony number and is no more than from 2 times that beam back spectrum, the result is judged to be feminine gender, and this sample of evidence finds no mutagenesis.

Residual and the environmental impact assessment of composite fertilizer

After illustrating that micro organism composite fertilizer is used, the residual condition of hazardous and noxious substances and the environmental influence that may bring thereof are intended carrying out A+E from following three aspects.

One, raw material and technical process

This bacterial manure is raw materials used, based on high-quality fertilizer, ground phosphate rock, potassium felspar sand: three's content, accounted for 95% of total amount, be aided with other composition of 5% again, all these compositions, except that fertilizer, phosphoric acid salt that complete set is common and silicoaluminate association mineral are as Ca ₅(PO ₄) 3F, K ₂OAl ₂O ₃6SiO ₂Deng, do not contain artificial synthetic chemical substance fully and contain the more special mineral of poisonous oxious component.The content of each heavy metal species in the raw material is natural natural tax storage, the i.e. general abundance of the earth's crust.So the composite fertilizer that utilizes these starting material to make can never give the environment pollution that (as the soil territory) brings objectionable impurities.

In addition, the manufacturing processed of this bacterial manure only is these raw-material pulverizing, granulation, drying course.Do not mix other material, do not produce new material yet, especially can not produce hazardous and noxious substances.Can affirm, utilize these raw materials, the objectionable impurities of special composition, special content can not contained in the composite fertilizer that adopts such technical process to make.

Two, finished product fertilizer oxious component is analyzed

According to the used raw material of this fertiliser production process and technical process as can be known: wherein the objectionable impurities that may exist is a heavy metal.Therefore, the finished product of this fertilizer is carried out random sampling, selects representational sample to carry out determining heavy metals, its result such as table one:

Table one

Project content	Cu	?Zn	?Pb	?Cd	Ni	As	?Cr	Hg
									Composite fertilizer's content	13.1	?152	?21.3	?0.290	6.44	16.9	?9.65	0.0638
Soil content	1～50	?10～300	?2.00～200	?0.01～7.00	50～500	1～50	?20～200	0.1～0.3
									The bacterial manure standard value	-	?-	?≤100	?≤3	-	≤30	?≤200	≤5
Measuring method	Atomic absorption	Atomic absorption	Atomic absorption	Atomic absorption	Atomic absorption	DDC-Ag	Atomic absorption	Atomic fluorescence

Measurement result shows, the heavy metal content in this fertilizer is equivalent to the content of general soil substantially, so use the accumulation that this fertilizer can not cause heavy metal, can not cause residues of harmful substances.In addition, the numeral of the row from table finds out that every determination data all is lower than the numerical value that national standard " microorganism (bacterium) fertilizer series products quality standard " is limited, so, aspect heavy metal content, be qualified product fully.

In addition, the heavy metal content in the soil generally is meant total amount, total amount and can give between the attitude and be in a kind of running balance.When external heavy metal enters soil, just increase the total content of soil within the specific limits, can not give attitude content and do not increase, therefore can not cause the pollution of crop.Total amount is not had a direct significance concerning crop, and can be the part that crop can absorb to attitude.Soil is referred to as environmental capacity to the sort buffer effect of external heavy metal in environmentalism.This running balance is the physical process of soil, the comprehensive embodiment of the partial circulating process of chemical process and biology.Because the existence of these processes causes in the soil and can give the heavy metal content of attitude, must be a more stable numeral far below total amount.So this fertilizer of long-term application can not cause effective accumulation of heavy metal yet.Can calculate according to measurement result and use composite fertilizer after 100 years, the heavy metal content that may increase only is equivalent to the fluctuation range (standard deviation) of soil total amount measured value, and nonsensical concerning the mean value of total amount measured value.So from statistical angle, this increment is to the not influence of this population mean of soil.

Three, use composite fertilizer after, the A+E of heavy metal content in soil, the crop

1, measurement result and background value (95% confidence region of X) or regional soil nature content

Manure trial in Changchun, Baicheng, prolong the limit, carry out coequally in Jilin, four, gathers the content of representative soil test heavy metal respectively, the results are shown in Table two: the continuous page table of going up

Project content	Cu	?Zn	?Pb	?Cd	?Ni	?Cr	?Hg	?As
									The area, Changchun
The measured value background value (X ± t0.05S)	14.5 10.5～ 21.5	?88.4 ?38.5～ ?75.0	?15.4 ?2.40～ ?30.3	?0.089 ?0.05～ ?1.05	?23.9 ?12.5～ ?29.5	?18.2 ?57.5～ ?62.5	?0.087 ?0.022～ ?0.112	?8.82 ?8.60～ ?10.0
									The Baicheng Prefecture
Measured value regional soil content	5.43 15.35	?75.3 ?53.84	?10.4 ?3～46	?0.071 ?0.05～1.05	?7.00 ?32.58	?6.86 ?51～159	?0.038 ?0.022～ ?0.112	?5.10 ?7.37～ ?11.3
									Yan Biandiqu
The regional natural content of measured value	20.6 13.7～ 25.1	?220 ?178～309	?30.2 ?14.1～38.3	?0.10 ?0.011～ ?0.250	?29.2 ?23.0～ ?86.1	?21.1 ?10.5～ ?40.8	?0.063 ?0.047～ ?0.099	?9.92 ?5.35～ ?13.3
									Jilin Area
Measured value background value * *	19.5 11.0～ 35.5	?118 ?46.5～ ?114	?19.7 ?3.80～ ?45.6	?0.078 ?0.05～ ?0.310	?29.3 ?14.5～ ?62.0	?6.53 ?58.7～ ?78.9	?0.038 ?0.024～ ?0.097	?7.23 ?7.37～ ?11.3
									Area, Siping City
The measured value background value	20.7 12.0～ 35.0	?100 ?49.0～ ?173	?25.9 ?2.70～ ?69.3	?0.080 ?0.05～0.50	?31.7 ?14.0～ ?37.5	?22.3 ?53.1～ ?67.6	?0.110 ?0.034～ ?0.520	?6.73 ?7.31～ ?11.3

In addition, on corresponding plot, gather Chinese cabbage, watermelon, black salted turnip, corn, paddy rice, Soybean and Other Crops sample respectively, made the mensuration of oxious component, the results are shown in Table three:

Crop monitoring result (mg/kg)

The project crop

Cu

?Zn

?Pb

?Cd

?Ni

?Cr

Hg

As

?NO2-N

?NO3-N

The continuous page table of going up of table continued on next page

Chinese cabbage	0.16	?1.4	?0.051	?0.0052	?0.079	?0.27	* not	?0.019	?0.0055	69
											Watermelon	0.14	?0.89	?0.090	Not	?0.10	?0.12	?0.006	?0.001	?0.003	52
Broccoli	0.16	?1.0	?0.090	?0.003	?0.42	?0.044	?0.002	?0.0020	?0.005	73
											Standard value	0.19**	?2.5**	?0.50	?0.05	?0.44	?0.28	?0.010	?0.50	?4.7	79
The corn measured value	2.4	?16	?0.10	?0.0026	Not	?0.49	Not	?0.043
											Background value (upper limit)	4.1	?21	?0.36	?0.0035	?0.50	?0.50	?0.012	?0.16
The paddy rice measured value	3.3	?17	?0.12	?0.0018	Not	?0.54	?0.01	?0.22
											Background value (upper limit)	5.1	?24	?0.58	?0.0047	?1.7	?1.16	?0.011	?0.26
The soybean measured value	6.2	?33	?0.066	?0.0021	?5.4	?0.46	Not	?0.039
											Background value (upper limit)	8.2	?37	?1.1	?0.0053	?5.5	?0.50	?0.016	?0.14

Annotate: the background value in the table two all draws from Changchun Institute of Geography Chinese Science Academy:

" background value of number of metal element in the main agricultural soil of MIDDLE NORTHEASTERN IN CHINA "

*: draw from Inst. of Forestry and Soil, Chinese Academy of Sciences and write " Northeast China soil ", Science Press, 1990.10;

*: be the measured value and the background value of rice soil

Vegetables in the table three " standard value " are drawn from Changchun Institute of Geography Chinese Science Academy, Science ﹠ Technology Center of eastern coal company and do: " nine vertical engineering-environments of Shulan mineral office influence the appraisal report book ", (main part) P177;

*: " not " promptly do not detect, and gets a half value of detection limit and participate in statistics when statistics;

*: Cu, Zu are suburb, the southeast, Beijing background values;

The background value of food crop draws from Changchun Institute of Geography Chinese Science Academy: " research of mineral element background value in the main food of middle area, northeast ".

By the said determination result, most as can be seen observed values all are lower than the higher limit of 95% fiducial interval of background value, can think and use composite fertilizer, do not cause the accumulation of eight kinds of elements (heavy metal) in soil; Do not cause eight heavy metal species and nitrite nitrogen, the accumulation of nitric nitrogen in vegetables, food crop yet.The measured value of having only Zn in the geographic soil in Changchun is a little more than background value.This may be that local soil difference causes on the one hand, and maximum possible is in recent years because the appearance of crop zinc deficiency, and the result of zinc is generally used in various places.Certainly, if can contain a certain amount of zinc in the composite fertilizer, this is a good thing to the demand of trace element zinc to satisfy crop.

2, use the environmental quality Present assessment of composite fertilizer

(1) estimates determining of emphasis

According to environmental impact assessment emphasis, principle of generality targetedly should be arranged, the emphasis of this evaluation should be placed on to be used in the accumulation of composite fertilizer to heavy metal-polluted soil.Because this bacterial manure mainly is to discharge the nutrition that crop is difficult to absorb in the raw material by the beneficial microorganism activity.Itself does not contain a large amount of water miscible compositions, thus the revolving with can not causing of bacterial manure to atmosphere, the surface water, phreatic influence, and only may cause influence to soil.Not containing maybe again in used raw material and the technical process adopted and can not produce poisonous and hazardous organism, can only be heavy metal so cause what environment was had disadvantageous effect.

(2) selection of evaluating

Soil assessment: Cn, Zn, Cu, Cd, Ni, Cr, Hg, As;

Crop is estimated: Cn, Zn, Pd, Cd, Ni, Cr, Hg, As, NO ₂-N, NO ₃-N.

3) calculating of evaluation number

(1) pollution index

(Si) be calculated as follows pollution index Pi with the measured value (Ci) of soil pollutant and standard value (background value): $\mathrm{Pi}=\frac{\mathrm{Pi}}{\mathrm{Si}}$

Calculation result sees Table four:

The pollution index table

Project area crop	Cn	?Zn	?Pb	?Cd	?Ni	?Cr	?Hg	?AS	?NO 2-N	?NO 3-N
											Changchun	0.67	?1.10	?0.51	?0.084	?0.81	?0.29	?0.77	?0.88
Baicheng	0.15	?0.89	?0.22	?0.067	?0.12	?0.043	?0.33	?0.45
											Prolong the limit	0.82	?0.71	?0.78	?0.40	?0.34	?0.52	?0.63	?0.75
Jilin	0.55	?1.00	?0.43	?0.25	?0.47	?0.08	?0.39	?0.64
											Siping City	0.59	?0.58	?0.37	?0.16	?0.85	?0.32	?0.21	?0.59
Corn	0.58	?0.76	?0.27	?0.74	?0	?0.96	?0	?0.27
											Paddy rice	0.65	?0.71	?0.21	?0.38	?0	?0.47	?0.91	?0.85
Soybean	0.76	?0.89	?0.06	?0.40	?0.98	?0.92	?0	?0.28
											Chinese cabbage	0.84	?0.56	?0.10	?0.10	?0.18	?0.96	?0	?0.04	?0.0011	?0.87
Watermelon	0.74	?0.36	?0.18	?0	?0.23	?0.43	?0.60	?0.022	?0.001	?0.66
											Broccoli	0.84	?0.40	?0.18	?0.06	?0.95	?0.16	?0.20	?0.004	?0.001	?0.92

(2) aggregative index

According to the purpose of this evaluation, be to use the pollution whether soil behind the bacterial manure is subjected to heavy metal really, root mean square method is adopted in the calculating of decision aggregative index, that is: $P=\underset{n}{\overset{1}{(-}}\underset{i=1}{\overset{n}{\mathrm{\Σ}}}{\mathrm{Pi})}^{1/2}$

Judge the size of pollution level according to aggregative index P, as P≤1, for uncontaminated; P＞1 is item for polluting; The P value is big more, pollutes heavy more.

According to top pollution index value, the result who calculates the comprehensive pollution indexes of each department, various crops respectively and judge whether to have polluted sees Table five.

Comprehensive pollution indexes

Soil or crop	Comprehensive pollution indexes	Whether pollute
			Changchun	0.710＜1	Uncontaminated
Baicheng	0.386＜1	Uncontaminated
			Prolong the limit	0.641＜1	Uncontaminated
Jilin	0.541＜1	Uncontaminated
			Siping City	0.508＜1	Uncontaminated
Corn	0.562＜1	Uncontaminated
			Paddy rice	0.600＜1	Uncontaminated
Soybean	0.654＜1	Uncontaminated

Four, after the long-term application composite fertilizer, the prediction of soil pollution situation

According to the present quality evaluation, soil, the crop of using as can be seen behind the 1-2 are all polluted.But the pollution of heavy metal has accumulating property in soil.Use after 1 year and do not pollute, but after using 10 years, after 100 years again how about? so, need the accumulation situation of heavy metal after the long-term application composite fertilizer is predicted.

1, with the comparison of soil content

According to the practical measurement of this fertilizer as can be known, heavy metal content wherein substantially all is equivalent to the content of general soil, sees Table one.So, no matter use how long, no matter consumption is much, can not change the relative content of heavy metal in soil, promptly can not cause the too much accumulation of heavy metal in soil.

2, relatively predicting according to input, work output

According to the particular case of this fertilizer, the consumption of per hectare is 500 kilograms, and by this fertilizer of 500 kilograms, the heavy metal amount of importing to soil is Cu6550mg, Zn7600mg.And the amount Cu that exports by agricultural-food is that 102000mg, Zn are 456000mg.The amount of input is much smaller than work output:

Input Cu-output Cu=-95450mg

Input Zn-output Zn=-380000mg

If calculate by using after 100 years, the Cu in the soil, Zn reduction just enlarge 100 times.

The input of other heavy metal, output situation from general trend, also are like this, and when not having other pollution if just use composite fertilizer, after for many years, the content of heavy metal in soil can not increase.

3, predict from the conversion of heavy metal soil

In the northern China arid area, the carbonate of heavy metal is comparatively common in soil.So, be example with carbonate, illustrate that the state of heavy metal in soil transforms, the growth and decline situation of prediction heavy metal in soil.

Heavy metal exists following equilibrium relationship in soil:

So according to solubility product constant K _Sp, can calculate the concentration of heavy metal ion in the soil solution:

Lg[M ²⁺]=lgK _Sp+ 18.4-lgP _Co22PH P wherein _Co2Be the CO in the soil air ₂Dividing potential drop is generally 0.3mbar.As being example with Pb, its solubility product constant K _Sp=8 * 10 ^-4So plumbous concentration in the soil solution is: $\lg \left[P_b^2\right]=\lg 8\×{10}^{-14}+18.4-\lg .0003-2\×7.39$

＝-13.10＋18.4＋3.523－14.78

＝-5.957

＝2.28×10 ^-4g/l

＝0.228PPm

From top calculating as can be seen:

1. the size of the heavy metal concentration in the soil solution (can give attitude) only is decided by K _Sp, P _Co2And PH, and irrelevant with the heavy metal in soil total amount;

2. the heavy metal amount in the soil solution is a comparison fixed numeric values much smaller than total amount.

So, even use composite fertilizer, bring soil a certain amount of heavy metal, also just increase the total amount of heavy metal in soil, and crop is polluted.

Five, conclusion

1, the civilian raw material of composite fertilizer does not contain special poisonous and harmful composition; Production process does not produce hazardous and noxious substances; Heavy metal content meets the regulation of national standard " microorganism (bacterium) fertilizer series products quality standard " fully in its finished product.

2, the content of heavy metal in the composite fertilizer is equivalent to the normal contents in the general soil, can not cause the accumulation of heavy metal in soil and the pollution of crop after using.

3, long-term application composite fertilizer can not increase the content of heavy metal-polluted soil.

4, use composite fertilizer, come detrimentally affect can for the surface water, underground water and other biozone.

Composite fertilizer is to utilize natural phosphorus potassium breeze, contains organic agriculture and industry waste and multifunctional bio catalyzer, adds an amount of adding and fills material through the formulated composite bio-fertilizer of special process processing.According to crop alimentary characteristics and soil fertility situation, cooperate a spot of attitude nutrient of giving in the product that has, what have mismatches.So composite bio-fertilizer of the present invention is that biology one organic inorganic three is the composite fertilizer of one, is incomparable than its high-tech content of single bacterial manure made from the microbial inoculum adding peat composed of rotten mosses and a spot of mineral carrier.

Its main effect and advantage show the following aspects:

1. can directly provide crop mineral nutrient and biologically active substance;

2. provide organic, improving soil fertility;

3. the mineral substance that decomposes organic and part indissoluble increases and the adjusting soil nutrient;

4. the saving chemical fertilizer cuts down expenses, and the protection soil fertility is free from environmental pollution;

5. the enhancing crop resistance is made better products, increasing both production and income.

Experimental study task, purpose mainly contain two.The first, the effect of increasing production of experimental study composite fertilizer on Jilin Province's staple crops MAIN SOILS is with chemical fertilizer of equal value, particularly with the comparative effectiveness of two ammoniums when equivalence drops into.The second, experimental study multifunctional bio catalyzer composite fertilizer comprises the soil agrochemistry proterties to soil fertility, micro-flora, the influence of soil enzyme activities.

Over 2 years, 77 experiment and demonstration points are tested in 14 cities and counties, 6 areas of the whole province, topped the western and eastern's main soil group in the whole province: Baijiang soil is arranged, the riverine rice soil of alluviation type, white slurry type grassy marshland rice soil, chernozem, light chernozem, thin layer middle level black earth, dark brown earth, light alkaline earth, careless black earth.100 tons of experiment and demonstration composite fertilizers use area and reach 300 hectares.Application process, composite fertilizer makes a mouthful base fertilizer.Consumption: soybean 300kg/ha, corn, rice 300～500kg/ha, beet 600kg/ha, tobacco 450-600kg/ha, Chinese cabbage 1-1.5 ton/ha (no chemical fertilizer join execute).Corn wherein, paddy rice, beet, tobacco, all with composite fertilizer+an amount of nitrogenous fertilizer, or nitrogen-phosphorus-potassium compound fertilizer does that base fertilizer is disposable to apply, and removes external the educating in the phase of paddy rice and do not topdress, and the two ammoniums+urea that drops into equivalence contrast, and being provided with of having do not have fertilizer and distinguishes (CK).Multifunctional bio catalyzer (composite fertilizer's microbial inoculum) is mainly as Chinese cabbage mouth fertilizer, consumption per hectare 7.5Kg.

The experimental study method mainly based on the field biology test demonstration, has arranged that with the Yan Bian institute of agricultural sciences basin carries paddy rice and green pepper test in the school.For inquiring into the influence of composite fertilizer to soil fertility, at the indoor agricultural voltinism shape of having made each test point soil, micro-flora, the urase of soil, Phosphoric acid esterase, the mensuration of catalase activity, and study the influence of composite fertilizer emphatically to quality product, tobacco grade and sugar-beet sugar content have been measured, Chinese cabbage vitamins C and NO ₃The influence of-N content.Investigation, record in the crop whole growing, have been done.

Two, the main result of experimental study

(1) composite fertilizer is to the effect of increasing production of soybean

1. composite fertilizer is to the effect of increasing production of soybean

Table 1-1 composite fertilizer is to soybean yield-increasing effect (the 93 pairs of sun, Tonghua, Shulan, Tongyu)

Handle	Mean yield (Kg/ha)	Average units increased in production (Kg/ha)	Average stimulation ratio (%)	Increment (unit/ha)	Increase income into (unit/ha)	Input/output
							Diammonium phosphate 100Kg/ha	2582.8	-	-	-	-	-
Composite fertilizer 300Kg/ha	3032.2	449.4	17.4	674.1	524.1	1∶3.5
							Composite fertilizer+fc	3077.3	494.5	19.7	791.2	641.2	1∶4.3

Result from 1-13 point of table sees, composite fertilizer and two ammoniums are of equal value drops into (150 yuan/ha) under the situation, mouthful base fertilizer each 1/2, composite fertilizer+a small amount of fc (composite fertilizer 71.4Kg), than singly executing two ammoniums volume increase 17.4-19.7%, increase income soybean 449.4-494.5Kg/ha, pure increasing income into being 524.1-641.2 unit, input-output ratio is 1: 3.5-1: 4.3.

See many 4.6 of sealing fertilizer and two ammonium single-strain legumen numbers, many 9.8 of single-strain grain number, many 23.6 of root nodule from the results species test, many 22.2 of radical, the blighted grain rate reduces 11.9%, falls leaves in advance ripe 2-3 days, statistical results show, 5 tests wherein have 4 points to reach 5% notable level.Other 1 point is not remarkable.When revolving, all remarkable with composite fertilizer+fc.So, at leaching thin layer black earth, Baijiang soil, light black calcium earth, middle fertility, manufacturing soybean can replace two ammoniums with composite fertilizer or the composite fertilizer+fc of 300Kg, and its effect of increasing production is more than or equal to the effect of increasing production of 100Kg two ammoniums.1 hectare of black soya bean sealing fertilizer 450Kg than executing two ammonium 150Kg volume increase 13.7%, increases black soya bean 2185.9Kg/ha.Net return is than Duo 526.8 yuan/ha with two ammoniums.

2. composite fertilizer is to the production-increasing function of corn

The 92-93 corn is used 12 test points of composite fertilizer, and 2 big area (4 hectares) demonstration pilot project, experiment and demonstration the results are summarized in table 2-1.

Table 2-1 composite fertilizer is to corn yield increasing effect (92-93 Siping City fills the Shulan, Changchun 12 test points altogether)

Handle	Output (Kg/ha)	Average volume increase (Kg/ha)	Average volume increase (%)	The of equal value input (unit/ha)	Pure increasing income into (unit/ha)	Input/output
							F (two ammonium 200Kg) urea 225Kg	?8934.6	-	-	525	-	-
Composite fertilizer 600Kg urea 225Kg	?9901.4	966.73	10.8	525	483.4	1∶0.92
							Composite fertilizer 600Kg fc325	?9595.4	560.8	6.9	525	280.4	1∶0.53

2-1 sees by table, the equivalence input (525 yuan/ha) under the situation, composite fertilizer+urea, 14 experimental tests (comprising 2 demonstration pilot projects), average specific increases production 10.8% to fertilize, increases corn 966.73Kg/ha, pure increasing income into 483.4 yuan/ha.The production-increasing function of composite fertilizer+fc is not so good as composite fertilizer+urea, but still increases production than chemical fertilizer.So per hectare is made base fertilizer with 600Kg bacterial manure, it is effective to make base fertilizer than 200Kg two ammoniums.Every 300Kg composite fertilizer effect is greater than 100Kg two ammonium effects.Every Kg composite fertilizer can increase corn 0.726-1.70Kg.

Each point shows that in breeding time investigation sealing fertilizer is handled, and the leaf number of plies of corn, plant height, big radical, stem are thick, spike length, fringe grain weight average are preponderated, bald sharp economization 1.1.cm, and moisture reduces by 2.7%, ripen in advance 2-3 days.Amount of increase in production 8.7-13.9% increases corn 600-700Kg, purely increases income 240-300 unit, and drought resisting, and precocity is disease-resistant, increases soil fertility.But the large-scale demonstration result is bigger than local habits fertilization (two ammonium 200-250Kg, urea 400Kg) amount of increase in production, reaches 17.3%.

In addition, done the corn test of composite fertilizer's high investment on the meadow chernozemic soil of suburb, Changchun, bacterial manure is executed 1.33-2.2T, and fc executes the 0.62-1.57 ton, and the result shows, 1.33 tons+0.62 ton fc of composite fertilizer, and corn yield is the highest, is 10123.5Kg/ha, volume increase 14.1%.Statistical results show, composite fertilizer can replace a part of fertilizer, and each ton bacterial manure is equivalent to 0.71 ton ternary compound action 10-9-8.

3. composite fertilizer is to the increasing production of rice effect

Made 3 large-scale demonstrations of 8 tests in 2 years altogether.The results are summarized in table 3-1.

Table 3-1 composite fertilizer is to increasing production of rice effect (93-92 prolongs 8 points such as limit, Tonghua, Shulan)

Handle	11 some mean yield kg/ha	11 points on average increase production kg/ha	Average stimulation ratio (%)	Drop into unit/ha	Pure increasing income into (unit/ha)	Input/output
							F (two ammonium 150kg urea 270kg)	?5085.3		-	?420
Composite fertilizer 300+ urea 270kg	?5466.7	?381.4	7.5	?420	?247.91	?1∶0.59
							Composite fertilizer 300+fc 385kg	?5373	?287.9	4.2	?420	?187.14	?1∶0.44

3 large-scale demonstration results show from 8 test points, the equivalence input (420 yuan/ha) under the situation, sealing fertilizer+an amount of chemical fertilizer (composite fertilizer+f), or (composite fertilizer+fc) all than the pure 4.2-7.5% that increases production to fertilize, average increment rice 287.9-381.4kg/ha, purely increase income unit into 187.14-241.91, input-output ratio be respectively 0.59: 1,0.44: 1,

Statistical results show, composite fertilizer+f increase yield significantly, and composite fertilizer+fc volume increase is not remarkable.Be per hectare sealing fertilizer 300kg+ urea 270kg, its production-increasing function is equal to or higher than the effect of executing two ammonium 150kg+ urea 270kg.

Composite fertilizer promotes the several 0.8/caves of rice tillering, and thousand seed weight increases 1.2 grams, the blighted grain rate reduces 10.3%.White many 4-5 of root bar.Heading 1-2 days in advance.

Saw prolonging the limit large-scale demonstration in 93 years, only with two ammoniums, the production field that the hydrogen ammonium topdresses as base fertilizer, general remaining green when it is due to become yellow and ripe late-maturing, blighted grain is many, has only the harvest of 20-30%, the big area total crop failure.And make base fertilizer with composite fertilizer 500-600kg, and chase after 450-550kg hydrogen ammonium, ripe 70-75%, harvesting rice 4650-5300kg illustrates that composite fertilizer has the effect that the raising crop is resisted low humidity, promotes the paddy rice early growth and quick tiller early ripe, volume increase.Therefore,, high and cold mountain area upper level short in breeding time add an amount of nitrogenous fertilizer with composite fertilizer and make base fertilizer, impose a small amount of nitrogenous fertilizer, are very important major issues during this area's Fertilization Structure changes.

4. composite fertilizer is to the production-increasing function of vegetables

(1), composite fertilizer is to potted plant green pepper effect of increasing production

This test is to be that culture farm is finished, tested and establish the ck-inorganic fertilizer in Jilin agricultural university soilization; The fc-10-9-8%NPK Nitrogen, Phosphorus and Potassium; An aseptic natural organic matter material and the breeze of composite fertilizer; Composite fertilizer B-have bacterium (composite fertilizer); Composite fertilizer's B+fc-bacterial manure+multiple fertile.Except that ck, each is handled impartial valency and drops into 0.012 yuan/basin.700 yuan/T of NPK Nitrogen, Phosphorus and Potassium, 500 yuan/T of composite fertilizer B bacterial manure.Every basin fertilising is calculated by of equal value the input.Three kinds of soil: Baijiang soil (acidity), meadow chernozemic soil (neutrality); Light alkaline earth (alkalescence), five processing, three repetitions, May 13 transplanted, every basin one strain.The kind farming is ground No. 4, receives fruit in two batches on July 10 and 30 days.Yield result is as table 4-1.

Show 4-1 composite fertilizer production-increasing function (937 Jilin agricultural university) to green pepper on different soils

Soil	Handle fc composite fertilizer composite fertilizer+fc+B composite fertilizer+B	Heavy (gram/basin) 77.4 121.5 161.0 154.4 of fruit	Volume increase (gram/basin)-44.1 83.6 77.0	％ - 57.0 108.0 99.5	Net return unit/basin-0.35 0.67 0.62	Input/output-1: 16.8 1: 31.8 1: 29.3	Morbidity (%)----
								Baijiang soil
Meadow chernozemic soil									Fc composite fertilizer composite fertilizer+fc+B composite fertilizer+B	59.5 139.8 198.3 135.4	- 80.3 138.8 75.9	- 135 233 128	?- ?0.64 ?1.11 ?0.61	- 1∶30.6 1∶52.9 1∶28.9	30 - 20 -
	Light alkaline earth	Fc composite fertilizer composite fertilizer+fc+B composite fertilizer+B	86.6 120.1 138.0 104.6	- 33.5 51.4 18.0	- 38.7 59.4 20.8	?- ?0.27 ?0.41 ?0.14	- 1∶12.8 1∶19.6 1∶6.8	30 - 25 -

4-1 result sees from table, executes composite fertilizer (fc) volume increase that natural organic materials breeze and composite fertilizer B (bacterial manure) drop into than equivalence.Wherein the neutral meadow chernozemic soil holds pride of place, and acid Baijiang soil accounts for second, the 3rd of light alkaline earth.Amount of increase in production, neutral meadow chernozemic soil is 128-233%, increases green pepper 75.9-138.8 gram/basin.Baijiang soil green pepper volume increase 57-108% increases green pepper 44.1-83.6 gram/basin.Light alkaline earth volume increase 20.8-59.4% increases table green pepper 18.0-51.4 gram/basin.From ratio for input and output, composite fertilizer all preponderates on three kinds of soil.From the green pepper sickness rate, execute the fc sickness rate and account for 30, add a spot of fc sickness rate in the bacterial manure and account for 20-25%, and sealing fertilizer B bacterial manure and natural material is anosis.Illustrate that the crop of using bacterial manure is stronger than the ability of the crop disease-resistant evil of using mineral fertilizer.

4-1 also sees from table, sees biological catalyst (B) effect separately, Baijiang soil, and meadow chernozemic soil is remarkable.Light alkaline earth has only composite fertilizer+fc+B to increase significantly than composite fertilizer (aseptic).In comprehensive test report (seeing 93 in a separate paper), compound bacterial fertilizer is in second batch of fruit picking (July 30) than composite fertilizer increase yield significantly, time length high temperature and rainy phase, and neutral meadow chernozemic soil two months, acid Baijiang soil, light alkaline earth had only about one month.

(2), biological catalyst (composite fertilizer's microbial inoculum) and composite fertilizer are to the Chinese cabbage effect of increasing production.

1. microbial inoculum (not adding natural organic-inorganic material) test is arranged in six vegetable grower families of suburb, Changchun meadow chernozemic soil, and per hectare is executed biological catalyst (composite fertilizer's microbial inoculum) 7.5kg and made a mouthful fertilizer, and other management is carried out as usual, surveys October 3 and produces the results list 4-2

Table 4-2 biological catalyst (composite fertilizer's microbial inoculum) is to Chinese cabbage production-increasing function (6 points in suburb, 93 Changchun)

Handle	Output (T/ha)	Units increased in production (T/ha)	％	Pure increasing income into (unit/ha)	Input/output
						No microbial inoculum	81.00	-	-	?-	-
Microbial inoculum is arranged	90.40	10.4	12.8	?2674	1∶89

Annotate: biological catalyst (composite fertilizer's microbial inoculum) valency: 1kg=4 unit; Chinese cabbage valency: 1kg=0.26 unit

4-2 sees from table, and per hectare is made a mouthful fertilizer with 7.5kg biological catalyst (composite fertilizer's microbial inoculum), and 10.4 tons/hectare of dishes are brightened in Chinese cabbage volume increase 12.8%, and pure increasing income into 2674 yuan dropped into (composite fertilizer's microbial inoculum) output ratio and be up to 1: 89.

2. composite fertilizer topdresses to the effect of increasing production of Chinese cabbage as Chinese cabbage

Topdress breeding time Chinese cabbage with 350-850kg/ha composite fertilizer, other measures are identical, compare with chemical fertilizer, in Siping City, Tonghua, Changchun 5 experimental tests the results lists 4-3.

Table 4-3 composite fertilizer is as the Chinese cabbage effect of increasing production (5 equalization points in 92-93 Siping City) of topdressing

Handle	Output (T/ha)	Volume increase (T/ha)	?％	Pure increasing income into (unit/ha)	Input/output
						F (chemical fertilizer) contrast F+ composite fertilizer	107.32	?-	?-
119.78	?12.46	?11.6	?2814.6	1∶6.6

Annotate: through t check P0.05df=8 t=2.31*

All Chinese cabbage anosis (the mashed group of water is rare) that does to topdress with composite fertilizer, there is not wizened leaf.Volume increase 11.6% is brightened 12.46 tons of dishes, pure increasing income into 2814.6 yuan.Remarkable in economical benefits.

3., composite fertilizer makes Chinese cabbage base fertilizer effect of increasing production

In Tonghua, ground tests such as Siping City, Changchun, Jilin, Gongzhuling.Two sets of plan: one is and chemical fertilizer two ammoniums, the ammonium nitrate composite fertilizer that drops into of equal value; The 2nd, use composite fertilizer's bacterial manure and chemical fertilizer non-equivalence input ratio separately, the results list 4-4, table 4-5.

The of equal value input of table 4-4 composite fertilizer and chemical fertilizer made base fertilizer to the Chinese cabbage production-increasing function

(5 points such as 92-92 Siping City are average)

Handle	Output (T/ha	Volume increase (T/ha)	％	Pure increasing income into (unit/ha)	Drop into (unit/ha)	Input/output	Annotating Chinese cabbage per kilogram price is 0.26 yuan
								F two ammonium 200+ ammonium nitrate 250kg	?61.44	-	-	-	?475	-

The continuous page table of going up of table continued on next page

Composite fertilizer's 600 ammonium nitrates 250	?64.30	2.86	?4.6	743.6	475	1∶1.6
								Composite fertilizer 600+fc250	?67.6	6.62	?10.12	1617.2	475	1∶3.4

4-4 sees from table, the equivalence input (475 yuan/ha) time, it is fertile in increasing production 4.6-10.12% with chemical fertilizer merely that composite fertilizer makes the base fertilizer mouth.Brighten dish 2.86-6.22 ton/hectare.Dish 4.767kg-10.37kg can be brightened in per kilogram composite fertilizer, increases income 743.6-1617.2 unit/ha, and 600kg composite fertilizer can replace two ammoniums of 200kg.And observe in breeding time Chinese cabbage, execute bacterial manure and reduce 2-10.5% than to fertilize Chinese cabbage sickness rate, the bag heart is fast, look just, moisture is few, wizened leaf is few.N is only inhaled in the chemical fertilizer processing, P nutrition does not have K.Chinese cabbage is good potassium crop, and per hundred jin of Chinese cabbages need N0.15 jin, P ₂O ₅0.07 jin, K ₂O0.2 jin, N: K ₂O=1: 1.33.Sealing fertilizer+a small amount of fc or N fertilizer, nutrient is complete, also has the active substance and the multifunctional bio catalyzer of plant-growth, so increase production disease-resistantly, quality is good.

The second cover testing program is composite fertilizer and chemical fertilizer non-equivalence input, and its result lists table 4-5 in.

Table 4-5 composite fertilizer and chemical fertilizer non-equivalence are done the influence (92 Gongzhuling, Siping City) of base fertilizer to Chinese cabbage output

Handle	Mean yield (T/ha)	Average volume increase (T/ha)	％	Drop into (unit/ha)	Pure increasing income into (unit/ha)	Input/output	Annotate 400 yuan/T of Chinese cabbage 0.16 yuan/kg composite fertilizer's bacterial manure
								F450-675 two ammonium 450-675 ammonium nitrates (kg/ha)	82.3	-	-	1137.5	-	-
Composite fertilizer 1000-1650 (kg/ha)	94.6	12.3	?14.94	553.3	?2552.17	1∶4.6

4-5 finds out that sealing fertilizer 1000-1650kg/ha makes Chinese cabbage base fertilizer from table, than chemical fertilizer volume increase 14.94%, brightens 12.3 tons of dishes, and the net return sealing fertilizer is 2552.17/ha.Economic benefit is considerable.And rule is identical, disease-resistant with 93 years, and moisture is few, and ground is warm and sunny, look just, anti-storage.

Part is gathered in the crops sample measure vitamins C and NO ₃-N content, the result is that sealing fertilizer can improve VITAMIN 0.9mg/kg, reduces NO ₃-N ₂O62mg/kg.This helps human and livestock health, reduces environmental pollution.

5. composite fertilizer is to the effect of increasing production of beet

This test is arranged in village beet sugar industry institute of Gongzhuling City model man experimental field.The luvic phaiozems middle fertility, preceding stubble corn.Three processing, three repetitions.20m ²The sub-district.Live management is with the production field.October 4 gathered in the crops.The results list 5-1.

Table 5-1 composite fertilizer is to beet production-increasing effect (93 models man village sugar grinds institute)

Handle	Average fast root output T/ha	Average volume increase T/ha	?％	Sugar content %	Increase sugared rate %	Produce sugar (T/ha)	Increase sugared %	Net return (unit/ha)	Input/output
										1. chemical fertilizer N100-ammonium nitrate P 2O 5100-coarse whiting K 2O100-Kcl (Kg/ha)	32.0	-	?-	13.26	-	?4.243	-	-	-
2. composite fertilizer's 500 ammonium nitrates 363 (kg/ha)	33.3	?1.3	?4.7	13.99	0.73	?4.659	?9.8	843	?1∶1.7
										3. composite fertilizer 500 fc368 (kg/ha)	35.3	?3.3	?11.3	13.87	0.61	?4.896	?15.4	1306	?1∶2.6

Annotate: fertilizer is of equal value to be dropped into: 507.6 yuan/ha, and beet root 0.16/kg.

Fertilizing method: fertile total amount 1/3 is made a mouthful fertilizer, and 2/3 makes base fertilizer.

5-1 sees that beet is made end mouth fertilizer with composite fertilizer from table, drops into chemical fertilizer volume increase 4.7-11.3% than equivalence, increases piece root 1.3-3.3 ton, and sugar degree 0.61-0.73 degree increases sugared rate 9.8-15.4%, increases sugared 0.416-0.653 ton.Through the LSR check, composite fertilizer+ammonium nitrate and chemical fertilizer comparing difference are not remarkable, and composite fertilizer+fc increases production significant difference.So composite fertilizer's bacterial manure+a small amount of composite fertilizer can replace NPK chemical fertilizer of equal value.

6. composite fertilizer is to the tobacco effect of increasing production

Test 92-93 is arranged in Changchun, prolongs the limit, three points in Dehui, five tests.1. if chemical fertilizer (F)-N ₄₅P ₂O ₅135, K ₂O135kg/ha, 1. composite fertilizer+F-composite fertilizer 600+ pin ammonium 90+K ₂SO ₄253.8kg/ha 3. composite fertilizer+fc-composite fertilizer 600+ composite fertilizer (10-9-8) 450kg/ha, sub-district area 30-40m ², 3-4 repetition, standardized management, the results list 6-1,6-2.Table 6-1 composite fertilizer is to tobacco effect of increasing production (93 Dehuis, prolong the limit)

The place	Handle	Output (kg/ha)	Volume increase (kg/ha)	％	The output value (unit/ha)	Pure increase income into	Throwing/product	Drop into (unit/ha)	Good smoke rate %
										The Dehui	F composite fertilizer+F composite fertilizer+fc	?2791.88 ?2633.25 ?2500.50	?291.38 ?132.75 ?-	?11.6 ?5.3 ?-	?7041.98 ?7635.38 ?6470.35	?6348.18 ?6942.18 ?5855.32	1∶10.2 1∶10.5 1∶11.0	?693.2 ?693.2 ?615.0	1.2 12.2 0.95
Prolong the limit	F composite fertilizer+F composite fertilizer+fc	?1975.80 ?2737.95 ?2554.80	?- ?762.15 ?579	?- ?38.60 ?29.30	?4507.5 ?6037.5 ?5562.0	?3814.3 ?5344.3 ?4947.0	1∶6.5 1∶8.7 1∶9.0	?693.2 ?693.2 ?615.0	- - -

Annotate: Dehui cigarette valency: F handles-2.439 yuan/kg, I+F=2.3926 unit/kg, composite fertilizer+fc=2.9054 unit/kg

Prolong limit cigarette valency: F and handle-2.281 yuan/kg, I+F=2.205 unit/kg, composite fertilizer+fc=2.177 unit/kg

Table 6-2, composite fertilizer and chemical fertilizer are to the influence (academy of agricultural sciences, 92 Changchun) of flue-cured tobacco product, quality

Project is handled	First-class cigarette %	Medium %	Go up medium %	Output (kg/ha)	Volume increase (kg/ha)	?％	Drop into (unit/ka)	Throwing/product	Average price (unit/kg)	Slow value
											Composite fertilizer 500kg/ha	10.55	?44.34	?54.89	1827.5	-112.5	-6.3	?360	?1∶9.34	1.84	?0.335
1/2 composite fertilizer+1/2f	7.47	?47.87	?55.34	2302.5	352.5	18.1	?360	?1∶12.3	1.92	?0.349
											F (contrast) N22.5 P 2O 567.5 ?K 2O67.5 ?(kg/ha)	7.09	?44.56	?51.65	1950.0	-	-	?360	?1∶10.0	1.86	?0.338

Annotate: the 1. 2. average (N.P of the pure nutrient of per kilogram of 400 yuan/T of composite fertilizer ₂O ₅.K ₂O) by 2.3 yuan

6-1 sees that Dehui black earth (middle thin layer) tobacco sealing fertilizer bacterial manure output is minimum from table, and chemical fertilizer is the highest, and chemical fertilizer increases production 6.6-11.6% than bacterial manure, increases cigarette 138.63-291.3kg.And the output value all be composite fertilizer+f the highest (7635.38 yuan/ha), than 593.4 yuan/ha of chemical fertilizer increment.The reason that causes this situation is because sealing fertilizer makes good smoke rate increase by 11%, so, though ultimate production is low, output value height, good in economic efficiency.

Prolonging the limit test-results proves, also is not only output height of sealing fertilizer, volume increase 579-762.15kg/ha, and amount of increase in production 29.3-38.6%, and also it is many to go up the medium grade cigarette rate, output value height.Remove fertilizer cost, sealing fertilizer is overcharged into 1530 yuan/ha than chemical fertilizer.

Table 6-2 shows that the sealing fertilizer good smoke rate is up to 10.55%.Chemical fertilizer is handled, and good smoke rate has only 7.09%, and better-than-average cigarette rate also is that sealing fertilizer bacterial manure is preponderated.Finally be that 1/2 composite fertilizer+1/2 processing is best,, increase cigarette 352.5%kg/ha than increasing production 18.1% to fertilize.

To sum up three parts of test materialss can draw as drawing a conclusion:

(1) composite fertilizer+an amount of chemical fertilizer, the tobacco production output value is the highest;

(2) composite fertilizer can increase first-class, last medium grade cigarette carelessness than the obvious raising tobacco quality of chemical fertilizer.Remarkable in economical benefits.

(2) composite fertilizer is to the influence of soil fertility

Emphasis is inquired into and to be applied after the composite fertilizer soil agronomic traits, soil microflora and catalase, and 63 soil samples are made in the influence of Phosphoric acid esterase, urease activity altogether, 819 assay determinations (not locating repetition), and it the results are summarized in table 7-1 to 7-6.

7-1 finds out from table, behind the soybean soil sealing fertilizer, the soil organism, full N, alkali-hydrolyzable nitrogen, available potassium increase to some extent, full N increases by 0.142%, alkali-hydrolyzable nitrogen increases 10.34mg/kg soil, and fast K increases 12.67mg/kg soil, and being equivalent to every mu increases alkaline hydrolysis N1.554kg, speedup K1.9kg, K1.91kg, fast P reduces 2.9mg/kg soil than the contrast of executing two ammoniums.

Micro-flora, the ratio of sealing fertilizer execute two ammoniums bacterial count increase by 14 * 10 ⁵, increasing by 34% relatively, actinomycetes increase by 18.78 * 10 ⁴, increase by 25% relatively.Fungi reduces 13.35 * 10 than handling to fertilize ³, reduce 29% relatively, to fertilize many of fungi number, this may with ammonium in two ammoniums through nitrification, pH value is descended, be more suitable for the cause of fungus breeding, so it is more than sealing fertilizer fungi number to execute ammonium.

Two, total seeing, the soybean sealing fertilizer, the soil agronomic traits, micro-flora and urease activity improve direction and develop towards helping soil fertility.Table 7-2, behind the corn field sealing fertilizer, the every analysis indexes of soil is all compared photograph (CK) and is executed increasing of two ammoniums, illustrates that also sealing fertilizer has active effect to soil fertility.

Behind the table 7-3 rice soil sealing fertilizer, the soil organism, full nitrogen, full phosphorus, available nutrient are than the slightly increase trend of executing two ammoniums, the bacterium actinomycetes should have raising mutually, and ratio two ammoniums that straight bacterium is sealing fertilizer+F reduce, composite fertilizer+FC is than the raising that has of two ammoniums, as for why being to await further discussion like this.H ₂O ₂Enzymic activity does not have considerable change, and the activity of Phosphoric acid esterase increases, and the direction that sign soil fertility is become better transforms.

Table 7-4 (1), potted plant green pepper is three kinds of soil analysis results show, sealing fertilizer is handled, the soil organism, full N/ alkali-hydrolyzable nitrogen, rapid available phosphorus, available potassium is all than there not being the fertile obviously raising of a right organic-inorganic thing (composite fertilizer) processing that contrasts (CK) and do not add bacterium, illustrate that sealing fertilizer has improved the soil basic fertility really, also the high-efficiency multi-function biological catalyst (composite fertilizer's microbial inoculum) of explanation adding has been brought into play effect, corresponding bacteria, actinomycetes, H ₂O ₂Enzyme, Phosphoric acid esterase, urease activity also improve thereupon.Also being higher than chemical fertilizer (Fc) and handling, also prove absolutely and use biology-organic and inorganic compound bacterial fertilizer, is the important measures of comprehensively increasing soil fertility.

Table 7-4 (2), table 7-4 (3) are that Chinese cabbage makes a mouthful fertilizer with biological catalyst (composite fertilizer's microbial inoculum) and composite fertilizer, and back soil analysis result topdresses, therefrom see particularly full nitrogen, the alkali-hydrolyzable nitrogen showed increased, doubling of doing to topdress is many, (bringing up to 252.8mg/kg from 116.7).Bacterial count increases by 26 times, and unwrapping wire also obviously improves.This proves further that again sealing fertilizer or microbial inoculum exchange the earth nutrient of breaking ground, and increases soil fertility, and creates benign ecological environment of soil and has all played active effect.

Table 7-5, table 7-6 is beet and tobacco results back soil analysis result, nutrient does not have considerable change, actinomycetes increase significantly than bacterium, fungi in the micro-flora, phosphatase activity behind the sealing fertilizer in the soil is preponderated, the raising of these two indexs whether with tobacco leaf on moderate proportions increase, sugar-beet sugar content increases that correlationship is arranged is to be worth the further problem of research.

(3), brief summary

1. from using the synergy fruit of the various crops of composite fertilizer, economic benefit, composite fertilizer's input/output:

1. effect of increasing production: soybean＞Chinese cabbage＞tobacco＞beet＞corn＞paddy rice.

2. economic benefit: Chinese cabbage＞tobacco＞beet＞soybean＞corn＞paddy rice.

3. the input-output ratio of composite fertilizer: Chinese cabbage＞soybean＞beet＞tobacco＞corn＞paddy rice.

Say that totally the effect of cash crop is best, Chinese cabbage most effective, and corn, paddy rice take second place in the field crop.

2. the effect of increasing income of using the various soil of composite fertilizer also is different from alkaline earth, and for various crops, the effect of increasing income of various soil also is not quite similar.

Composite fertilizer generally based on base fertilizer, mouthful fertilizer, its effect of increasing production is good; Biological catalyst is applied to Chinese cabbage, and per hectare 7.5kg is respond well.

4. use composite fertilizer and can improve the crop product quality, improve the resistance of crop.Composite fertilizer's bacterial manure can improve the Vitamin C content of Chinese cabbage, and the last medium tobacco leaf ratio of the sugar content of storage tolerance and beet and tobacco just.Moisture and the NO of Chinese cabbage can fall ₃The diseased fruit rate of-N content and green pepper, the blighted grain rate of paddy rice, the not plump pod rate of soybean, the bald point of corn makes corn, paddy rice ripen in advance 3-4 days, Chinese cabbage is early rolled into a ball, early goes public 3-5 days.

5. use composite fertilizer's soil fertility and develop toward the good aspect, make improving by the voltinism shape of soil, organic content slightly improves, and full nitrogen base is separated nitrogen content and risen, and bacterium, actinomycetes number increase in the soil, Phosphoric acid esterase, and the arteries and veins enzymic activity strengthens, the H of minority soil ₂O ₂Enzymic activity also strengthens, and the soil available potassium improves, and illustrates that using biological organic-inorganic composite fertilizer is the important measures of comprehensively increasing soil fertility.Table 7-1 soybean is used composite fertilizer's bacterial manure and receives back change of soil nutrient situation

The test point	Handle	The PH water logging	Organic (%)	Full nitrogen (%)	Full phosphorus (%)	Alkali-hydrolyzable nitrogen (mg/kg)	Rapid available phosphorus (mg/kg)	Available potassium (mg/kg)
									Lotus flower township, Shulan (Baijiang soil)	F (two ammoniums) composite fertilizer composite fertilizer+fc	?5.75 ?5.90 ?5.75	?2.170 ?2.150 ?2.310	?0.1172 ?0.1161 ?0.1293	?0.040 ?0.039 ?0.043	?116.7 ?116.2 ?128.8	?10.9 ?6.7 ?10.2	?80.8 ?79.3 ?98.2
Tonghua City Dongchang District (atteration)	F composite fertilizer composite fertilizer+fc	?5.40 ?5.28 ?5.28	?3.800 ?3.790 ?3.860	?0.2128 ?0.2122 ?0.2162	?0.124 ?0.123 ?0.121	?206.6 ?196.9 ?226.1	?30.5 ?22.8 ?25.5	?38.1 ?58.5 ?68.5
									Yang Jingzi township, Tongyu County (light chernozem)	F composite fertilizer composite fertilizer+fc	?8.60 ?8.65 ?8.75	- - -	- - -	- - -	?102.5 ?98.7 ?112.6	?0.55 ?1.25 ?1.82	?78.3 ?80.8 ?98.3
The yellow black earth in hillock, two positive laterite village	F composite fertilizer	?5.4 ?5.7	?1.440 ?1.540	?0.0806 ?0.0862	?0.046 ?0.038	?102.3 ?117.2	?13.8 ?8.9	?83.3 ?95.7

Table 7-1 is continuous

The test point	Bacterium * 10 5	Actinomycetes * 10 4	Fungi * 10 3	H2O2 enzyme (0.1NK MnO 4ml/g)	Phosphoric acid esterase (phenol mg/g)	Urase (NH 4-N (mg/100g±)
							Lotus flower township, Shulan (Baijiang soil)	?61.06 ?58.39 ?72.50	?51.77 ?58.39 ?66.66	?38.16 ?24.1 ?39.36	?0.112 - ?0.114	2.206 - 2.199	8.002 - 8.817
Tonghua City Dongchang District (atteration)	?43.23 ?80.81 ?68.33	?56.74 ?102.79 ?72.37	?62.15 ?44.67 ?60.59	- - -	- - -	- - -
							Yang Jingzi township, Tongyu County (light chernozem)	?19.42 ?23.43 ?28.51	?57.29 ?115.83 ?87.48	?20.48 ?7.7 ?6.61	- - -	- - -	- - -
The yellow black earth in hillock, two positive laterite village	?40.21 ?52.67	?134.79 ?153.62	?62.28 ?43.89	- -	- -	- -

Table 7-2 corn sealing fertilizer bacterial manure is to soil agrochemistry affect trait (93 Jilin agricultural universities mensuration)

The test point	Handle	PH (water logging)	Organic (%)	Full nitrogen (%)	Full phosphorus (%)	Alkali-hydrolyzable nitrogen mg/kg	Rapid available phosphorus mg/kg
								Siping City Tiedong District (dark brown earth)	CK	?5.96	?1.823	?0.0984	?0.056	?98.2	?7.3
F	?5.84	?1.820	?0.0983	?0.068	?100.1	?9.4
							Composite fertilizer+F		?5.99	?1.843	?0.1112	?0.059	?110.9	?8.9
Composite fertilizer+fc	?6.0	?1.853	?0.1001	?0.063	?112.3	?10.6

Table 7-2 is continuous

The test point	Available potassium mg/kg	Bacterium * 10 5	Actinomycetes * 10 4	Fungi * 10 3	?H 2O 2Enzyme (0.1 NKMnO 4ml/g	Phosphoric acid esterase (phenol mg/g)	Urase NH 4-N mg/100g±
								Siping City Tiedong District (dark brown earth)	112	?-	-	-	?-	-	-
103	?53.92	42.16	8.93	?0.0925	1.851	6.2504
							121		?82.73	53.97	11.20	?0.0962	2.098	8.1474
131	?-	-	-	?-	-	-

Table 7-3 paddy rice sealing fertilizer is to the influence (93 Jilin agricultural university) of soil fertility

	Handle		PH (water logging)	Organic (%)	Full nitrogen (%)	Full phosphorus (%)	Alkali-hydrolyzable nitrogen mg/kg	Rapid available phosphorus mg/kg
									Tonghua City Dongchang District (atteration)		F	5.9	?2.61	?0.1409	?0.0490	?131.7	?2.9
Composite fertilizer+F	5.9	?2.89	?0.1503	?0.0550	?137.1	?2.8
							Composite fertilizer+fc	5.8			?2.88	?0.1526	?0.061	?133.3	?3.9
Lotus flower township, Shulan City (Baijiang soil)		F	5.65	?3.83	?0.2030	?0.0408										?145.4	?8.6
							Composite fertilizer+F	5.65	?3.83	?0.2030	?0.0485	?148.3	?12.6
		Composite fertilizer+fc	5.7	?3.82	?0.2025	?0.0512								?155.1	?19.7
							The Dragon Well tea township Yan Bian institute of agricultural sciences (grassy marshland type atteration)		F	5.80	?3.32	?0.1759	?0.0551			?125.9	?7.7
Composite fertilizer+F	6.10	?3.34	?0.1758	?0.0470	?137.6	?11.71
									Composite fertilizer+fc	-	-	-	-	-	-

Table 7-3 is continuous

	Available potassium mg/kg	Bacterium * 10 5	Actinomycetes * 10 4	Fungi * 10 3	?H 2O 2Enzyme (0.1NKMn O 4ml/g)	Phosphoric acid esterase phenol mg/g	Urase (NH 4-N ?mg/100g±)
								Tonghua City Dongchang District (atteration)	48.6	?32.74	?89.30	?2.39	-	-	-
51.1	?45.21	?96.03	?1.81	-	-	-
							63.5		?50.56	?118.41	?4.13	-	-	-
Lotus flower township, temple, Shulan (Baijiang soil)	78.3	?20.73	?46.52	?6.44	-	-									-
							98.2	?34.16	?56.36	?4.44	-	-	-
	93.2	?44.51	?80.19	?13.16	-	-								-
							The Longjing City Yan Bian institute of agricultural sciences (grassy marshland type atteration)	170.5	?97.86	?75.99	?12.23	0.1635	?1.924		?4.0519
170.0	?158.98	?123.85	?6.41	0.1725	?4.170	?5.3242
								?-	-	-	-	-	-	-

Table 7-4 (1) sealing fertilizer bacterial manure is to the soil fertility influence (93 Jilin agricultural university) of plantation green pepper

Soil type (basin sanction) Baijiang soil	Handle	PH (water logging)	Organic %	Full nitrogen %	Full phosphorus %	Alkaline hydrolysis N mg/kg	Rapid available phosphorus mg/kg	Available potassium mg/kg
									ck	6.2	?1.3692	?0.0767	?0.0214	?74.9	?5.0	?83.3
	fc	5.85	?1.3919	?0.0779	?0.0744	?81.7	?107.8	?356.9
									Composite fertilizer	6.86	?1.5643	?0.0876	?0.0736	?81.7	?14.0	?113.0
	Composite fertilizer+f+B	6.25	?1.4231	?0.0797	?0.0764	?87.0	?38.6	?123.0
									Composite fertilizer+B	6.80	?1.5522	?0.0869	?0.0758	?89.0	?27.8	?122.0
	Meadow chernozemic soil	ck	6.9	?2.9999	?0.1556	?0.0492	?118.1	?13.6									?162.6
fc									5.90	?3.0963	?0.1610	?0.0948	?138.1	?128.4	?385.8
		Composite fertilizer	6.90	?3.1012	?0.1644	?0.0904	?128.3	?17.8								?172.6
Composite fertilizer+fc+B									6.25	?3.1001	?0.1643	?0.0937	?136.1	?53.2	?197.3
		Composite fertilizer+B	6.95	?3.0778	?0.1631	?0.0925	?139.0	?18.8								?204.8
Saline-alkali soil									ck	9.1	?1.7235	?0.0965	?0.0338	?89.5	Vestige		?80.8
	fc	8.15	?1.6595	?0.0929	?0.0833	?99.2	?79.4	?291.
									Composite fertilizer	8.45	?1.9273	?0.1079	?0.0807	?92.8	?20.0	?98.2
	Composite fertilizer+fc+B	8.90	?1.9112	?0.1070	?0.0829	?93.3	?73.0	?152.7
									Composite fertilizer+B	8.85	?2.2485	?0.1259	?0.0819	?98.0	?19.0	?108.1

Table 7-4 (1) is continuous

Soil type (potted plant) Baijiang soil	Bacterium * 10 5	Actinomycetes * 10 4	Fungi * 10 3	Catalase 0.1NKMnO 4ml/g	Phosphoric acid esterase phenol mg/g	Urase NH 4-N ?mg/100g±
							31.12	?44.47	?7.17	-	-	-
	29.33	?62.39	?16.62	?0.0738	?1.409	?3.2376
							59.49	?52.49	?5.41	-	-	-
	86.69	?168.69	?3.71	?0.1133	?1.88	?9.0958
							126.61	?147.45	?10.30	?0.1275	?3.474	?13.7268
	Meadow chernozemic soil	25.01	?42.93	?12.17	-	-							-
24.35							?41.94	?7.17	?0.1065	?3.076	?7.7792
		47.57	?95.05	?3.73	-	-						-
53.06							?181.33	?6.87	?0.1365	?6.917	?17.4106
		302.91	?135.61	?6.59	?0.1120	?6.808						?17.9686
Saline-alkali soil							54.64	?26.03	?4.05	-	-		-
	31.73	?98.69	?3.10	?0.1388	?2.206	?14.8442
							31.14	?118.52	?4.58	-	-	-
	56.61	?107.74	?5.03	?0.1562	?1.134	?16.5180
							278.60	?161.39	?6.54	?0.1725	?1.446	?15.8357

Table 7-4 (2) Chinese Cabbage composite fertilizer microbial inoculum is to soil fertility influence (93 Jilin agricultural university)

The test point	Handle	The PH water logging	Organic (%)	Full nitrogen (%)	Full phosphorus (%)	Alkali-hydrolyzable nitrogen (mg/kg)	Rapid available phosphorus (mg/kg)	Available potassium (mg/kg)
									Effect district, Changchun	There is bacterium aseptic	?7.1	?2.6700	0.1628	?0.086	?147.8	?86.2	?160.2
?7.2	?2.6140	0.1479	?0.074	?143.4	?72.2	?131.8

Table 7-4 (2) is continuous

The test point	Bacterium * 10 5	Actinomycetes * 10 4	Fungi * 10 3	?H 2O 2Enzyme 0.1 NKMnO 4ml/g	Phosphoric acid esterase phenol mg/g	Urase NH 4-N ?mg/100g±
							Effect district, Changchun	426.70	?402.70	?22.35	?0.1875	?3.402	?28.2346
417.70	?261.80	?33.25	?0.1450	?3.039	?21.7625

Table 7-4 (3) composite fertilizer bacterial manure is the influence (93 Jilin agricultural university) to Chinese cabbage soil agrochemistry proterties of topdressing

The test point	Handle	The PH water logging	Organic (%)	Full nitrogen (%)	Full phosphorus (%)	Alkali-hydrolyzable nitrogen (mg/kg)	Rapid available phosphorus (mg/kg)	Available potassium (mg/kg)
									Effect district, Changchun	F F+ composite fertilizer	5.25	?2.65	?0.1378	?0.057	?106.7	?37.8	?83.3
5.70	?2.67	?0.1442	?0.060	?252.8	?38.4	?89.2

Table 7-4 (3) is continuous

The test point	Bacterium * 10 5	Actinomycetes * 10 4	Fungi * 10 3	?H 2O 2Enzyme 0.1 NKMnO 4ml/g	Phosphoric acid esterase phenol mg/g	Urase NH 4-N ?mg/100g±
							Effect district, Changchun	13.29	?71.50	?4.2	?0.1075	?3.3230	?22.3204
346.95	?165.38	?34.69	?0.1075	?7.047	?23.8825

Table 7-5 composite fertilizer bacterial manure influences beet soil fertility

The test point	Handle	The PH water logging	Organic (%)	Full nitrogen (%)	Full phosphorus (%)	Alkali-hydrolyzable nitrogen (mg/kg)	Rapid available phosphorus (mg/kg)	Available potassium (mg/kg)
									Village beet institute of model man	Ck F composite fertilizer+NH4NO3 composite fertilizer+fc	?6.8 ?6.5 ?6.30 ?6.60	?2.9452 ?3.0560 ?3.0426 ?2.9693	- - - -	- - - -	?134.2 ?132.2 ?121.5 ?141.3	?65.0 ?135.0 ?66.8 ?78.2	?173.8 ?203.7 ?173.8 ?163.9

Table 7-5 is continuous

The test point	Bacterium * 10 5	Actinomycetes * 10 4	Fungi * 10 3	?H 2O 2Enzyme 0.1 NKMnO 4ml/g	Phosphoric acid esterase phenol mg/g	Urase NH 4-N ?mg/100g±
							Village beet institute of model man	?113.3 ?153.9 ?125.5 ?167.7	?83.68 ?79.60 ?87.10 ?94.80	?40.8 ?42.1 ?43.6 ?59.6	- ?0.1725 - ?0.1762	- ?0.1888 - ?3.133	- ?20.7582 - ?20.7582

Table 7-6 uses the tobacco ground soil fertility situation (93 Jilin agricultural university) of composite fertilizer's bacterial manure

The test point	Handle	The PH water logging	Organic (%)	Full nitrogen (%)	Full phosphorus (%)	Alkali-hydrolyzable nitrogen (mg/kg)	Rapid available phosphorus (mg/kg)	Available potassium (mg/kg)
									The Longjing City Yan Bian institute of agricultural sciences	F	?5.32	-	-	-	?98.7	?42.9	?237
Composite fertilizer+F	?5.52	-	-	-	?123.0	?48.5	?359
								Composite fertilizer+fc		?5.30	-	-	-	?118.1	?24.1	?237

Table 7-6 is continuous

The test point	Bacterium * 10 5	Fine rule bacterium * 10 4	Fungi * 10 3	?H 2O 2Enzyme 0.1 NKMnO 4ml/g	Phosphoric acid esterase phenol mg/g	Urase NH 4-N mg/100g±
							The Longjing City Yan Bian institute of agricultural sciences	?10.77	?91.7	?21.50	?0.0938	?5.779	2.3561
?12.82	?126.2	?20.94	?0.1288	?5.866	14.956
						?14.25		?203.1	?15.49	-	-	-

Claims (9)

1, a kind of new microbial composite organic-inorganic fertilizer manufacture method comprises:

Utilize nature and industrial or agricultural and municipal domestic organic refuse waste to be raw material, adopt physics, chemical process, make it part and be degraded to monose, disaccharide, trisaccharide, tetrose as carbon source, protein degradation be peptide class and amino acid as nitrogenous source, and add small amounts of inorganic nitrogen nitrogenous source as a supplement;

Utilize above-mentioned carbon, nitrogenous source, breeding yeast and useful fungi produce a large amount of single cell proteins, make it to become the organic nitrogen nutrition in the compound manure; Add the subtilis of energy decomposing protein, make it to become quick-acting nitrogen;

Utilize above-mentioned carbon, nitrogenous source breeding black-koji mould, making it at pH value is to produce oxalic acid under the neutral condition, constraint phosphorus with part crystal lattice state in the activating phosphorite powder, become rapid available phosphorus, make it to become the plain nutrition of phosphorus in the compound manure, the phosphate use inorganic phosphorus bacteria that is fixed in the constraint phosphorus of the crystal lattice state that all the other are not activated and the soil continues activation and make it to continue to increase rapid available phosphorus in soil;

Utilize above-mentioned carbon source and gaseous nitrogen breeding silicate bacteria, make it to activate the mineral substance such as potassium felspar sand or the alunite pulvis that contain lattice attitude potassium oxide, making it part becomes available potassium; The potassium that is fixed in remaining lattice attitude potassium oxide and the soil continues to be activated by silicate bacteria, to increase the available potassium in the soil;

Based on above nitrogen, phosphorus, potassium nutrition and microbial metabolites and the remaining organic matter that forms through microbial process, with the nitrogen of inorganic fertilizer, phosphorus, potassium and an amount of middle amount, trace element, rare no element, rare earth element is adjusted mutually and replenish, and forms microbial organic/inorganic compound fertilizer.

2, the described a kind of new microbial composite organic-inorganic fertilizer manufacture method of claim 1, wherein said organic substance raw material comprises and is rich in xylogen, the crop tangerine bar of Mierocrystalline cellulose, hemicellulose, pectin substance comprises corn tangerine, soybean tangerine, cotton tangerine, straw, wheat straw, fodder grasses, weeds; The slag class comprises beet pulp, bagasse; The cot class comprises rice husk, Exocarpium cocois (Cocos nucifera L), tung oil shell, Pericarppium arachidis hypogaeae, sunflower shell; Wood machining residues comprises sawdust, wood shavings, leaf; Be rich in peat composed of rotten mosses class, the peat class of humic acid; Brown coal, weathered coal class, the city organic matter rubbish class after the letter sorting; The Xylitol slag that Xylitol factory produces; The furfural dregs that furfural factory produces; Rich in starch and proteinic slag class comprise bean dregs, vermicelli slag, fecula; The remaining puffed soybean in oil expression back comprises dregs of beans, sunflower seed dregs, the Semen Brassicae campestris dregs of rice, peanut meal, cottonseed meal, castor seed meal, Semen Ricini industry, the tung oil dregs of rice; Industrial fermentation waste material melt cinder, poor class comprise that brewer's grains, distillers ' grains, alcohol are poor, residue of monosodium glutamate, acid-sludge, residue of soya, ferment glycerin slag, fermentation solvent slag, citric acid fermented slag, the amino acid sludge of fermentation, high fructose syrup screenings and people, animal, poultry manure.

3, the described a kind of new microbial composite organic-inorganic fertilizer manufacture method of claim 1, in the wherein said mineral raw material, phosphorus ore containing comprises: phosphatic rock, phosphorite, potassium-bearing mineral, potassium felspar sand, alunite, mica; The micro-mineral of amount comprise rhombspar, Wingdale, lime dinas, the tremolite, diopside, magnesium borate ore, Tremoliteor Tremoliteasbestos, serpentine, calcite, aragonite, giobertite in containing; Contain heavy metal in iron, manganese, copper, zinc, molybdenum, boron rock, the zeolite ore deposit faced below the intermediate value; Contain the medical stone ore deposit of yttrium, the mishmetal that contains rare earth element, rare earth nitrate or contain the useless smelting slag of rare earth element.

4, the described a kind of new microbial composite organic-inorganic fertilizer manufacture method of claim 1, wherein make Mierocrystalline cellulose, hemicellulose, pectin substance, starchiness class in the organic matter material, under acidic conditions, under 100 ℃～400 ℃ temperature condition, under the pressure condition of 0.1MPa～3.0MPa, make it part and generate monose, disaccharide, trisaccharide, tetrose; Make the protein transduction in the organic matter become peptide class and amino acid; For the low organic matter of protein content, use inorganic nitrogen, comprise that carbon ammonium, urea, sulphur ammonium replenish, for microbial reproduction provides a large amount of carbon source and nitrogenous sources.

5, the described a kind of new microbial composite organic-inorganic fertilizer manufacture method of claim 1, wherein utilize above-mentioned carbon source and nitrogenous source, breed yeast or useful fungi according to a conventional method, the accumulation single cell protein, or mycelium protein, as the organic nitrogen nutrition in the composite fertilizer, also the inorganic nitrogen in the chemical fertilizer can be changed into protein attitude nitrogen with this, make chemical fertilizer innoxious; Before granulation, add to produce the subtilis of proteolytic enzyme, make it in soil, to resolve into polypeptide, amino acid, until ammonia.For farm crop provide quick-acting nitrogen; Wherein the amount of the subtilis of Jia Ruing is the bacterium liquid (20～10,000,000,000/ml) of 1 ‰～10% (weight percent) of adding carrier amount.

6, the described a kind of new microbial composite organic-inorganic fertilizer manufacture method of claim 1, wherein, the generation of rapid available phosphorus, be that the oxalic acid activation that the black-koji mould with 1 ‰～10% (weight percent) of ground phosphate rock produces under the pH value neutrallty condition contains ground phosphate rock, make it part and change into rapid available phosphorus, the particle diameter of required ground phosphate rock need reach 80～250 orders; Remaining invalid phosphorus before granulation, adds phosphate-solubilizing bacteria again, as adds 1 ‰～10% bacillus megaterium, makes it to continue in soil phosphorus decomposing.

7, the described a kind of new microbial composite organic-inorganic fertilizer manufacture method of claim 1, wherein, the generation of available potassium, be to utilize the silicate bacteria of potassium-bearing mineral 1%～10% or other microorganism that ability of dissolving potassium is arranged to carry out potassium decomposing, remaining invalid potassium, before granulation, add potassium-bearing mineral 1 ‰～10% silicate bacteria again, make it in soil, to continue potassium decomposing; Potassium decomposing simultaneously, contained moderate-element comprises that silicon, calcium, magnesium, sulphur and trace element, rare elements, rare earth element also are activated simultaneously, or rare, the rare earth element breeze of amount trace makes it to activate simultaneously in adding simultaneously.

8, the described a kind of new microbial composite organic-inorganic fertilizer manufacture method of claim 1, wherein, the microorganism that the back adds is earlier with after fine perlite powder, the expanded peat dust absorption of expanded ground silica, join in organic, the mineral material again; Contain the organic mineral part and the organic-inorganic matter and the chemical fertilizer part that do not contain microorganism of microorganism, granulation respectively mixes between particle then, makes chemical fertilizer not contact microorganism fully, makes microorganism keep original activity, and is not subjected to the influence of chemical fertilizer.

9, the described a kind of new microbial composite organic-inorganic fertilizer manufacture method of claim 1, wherein, the ratio of each component is 0.1～5% microorganism: 20～60% organic matter: the inanimate matter of surplus comprises major element N, P, K, moderate-element Ca, Mg, S, Si, trace element, rare elements, rare earth element.

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