CN104478498A - Microbial preparation for ecological restoration of medium and low-yield field and preparation method of microbial preparation - Google Patents
Microbial preparation for ecological restoration of medium and low-yield field and preparation method of microbial preparation Download PDFInfo
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Abstract
The invention provides a microbial preparation for ecological restoration of a medium and low-yield field. The microbial preparation comprises the following components: a distiller grain fermentation product, bacillus cereus, sodium humate and potassium fulvate, wherein a preparation method of the distiller grain fermentation product comprises the steps of 1, mixing the preparation raw material components according to a certain weight ratio, and sealing; and 2, fermenting at any temperature or temperature interval ranging from 30 DEG C to 35 DEG C for 10-15 days to obtain the distiller grain fermentation product. The invention also discloses a preparation method of the microbial preparation. The preparation method comprises the steps of mixing the distiller grain fermentation product, bacillus cereus, sodium humate and potassium fulvate according to a certain weight ratio, and uniformly stirring to obtain the microbial preparation. The microbial preparation provided by the invention has long effectiveness and is ecological, environment-friendly and suitable for large-scale popularization and application and capable of ensuring that the productivity of the medium and low-yield field is stably improved; and in addition, the preparation method is simple, easy to realize, low in cost and suitable for large-scale production.
Description
Technical field
The present invention relates to a kind of microbial preparation, particularly relate to a kind of microbial preparation for middle-and-low-yielding fields restoration of the ecosystem, particularly, is a kind of microbial preparation for middle-and-low-yielding fields restoration of the ecosystem and preparation method thereof.
Background technology
Middle-and-low-yielding fields, it is the barrier factors that there are one or more restriction agriculture productions in a kind of soil, cause yield per unit relatively low and the arable land of shakiness, comprise accumulated water incobation type, ore deposit poison contamination type, nutritional deficiency fertility-betterment type, barren incrassation type, quality modified form, hillside fields change ladder type etc.No matter be any, for taking food as the mankind in sky, the big area of middle-and-low-yielding fields exists, and has had a strong impact on the output of food crop, especially for some Special section, even cannot ensure the life of local people.
Although vast territory and abundant resources in China, cultivated area total amount is also very considerable, wherein has the middle-and-low-yielding fields of 78.5%, and middle product field area accounts for 37.3%, and low-yield land area accounts for 41.2%.Visible, the middle-and-low-yielding fields of high coverage rate, remain the great burden affecting China's gross value of agricultural production.Therefore, the barrier factors for different soils carries out upgrading low-and medium-yield farmland, improves fertility, is to be badly in need of at present solving.
At present, although had many methods for upgrading low-and medium-yield farmland for implementing, effect is unsatisfactory.Such as:
CN1100586A discloses a kind of method of soil of improving the low and medium-yield farmland, be included in field and dig multiplex ditch and waterways, ensure that multiplex ditch abutment wall has at least one side to flush with mud face, although this more original method serves certain improvement effect to a certain extent, but in the long run, unsatisfactory, improving effect also has limitation;
CN103910580A discloses a kind of general PE special fertilizer being applicable to Huang-Huai-Hai middle-and-low-yielding fields, its basic raw material comprises organic substrate, inorganic matrix, nitrogenous fertilizer, phosphate fertilizer, potash fertilizer and trace element chelated dose, add in sesbania by disposable, realize the improvement of middle-and-low-yielding fields, although simply, conveniently, but, this special fertilizer contains chemical fertilizer, causes environmental pollution, and the utilization ratio of fertilizer is not high in addition, cause waste, be unfavorable for promoting and practicality.
In fact, the transformation of middle-and-low-yielding fields is not merely improve to work as annual production, but will be conceived to the soil improvement of essence, improves farmland comprehensive productive capacity, and ensures it is realize under the prerequisite of ecological recovery.Therefore, a kind of effectively for the method for middle-and-low-yielding fields ecological recovery, be current people in the urgent need to.
Summary of the invention
In order to improve the deficiency of prior art in actual production, application, meet people for the hope effectively improving middle-and-low-yielding fields, the invention provides a kind of microbial preparation for middle-and-low-yielding fields restoration of the ecosystem and preparation method thereof, described microbial preparation is obtained as main ingredient preparation by distillers ' grains fermented product, not only can ensure that the productivity of middle-and-low-yielding fields steadily improves, there is long-range ground validity, and ecological, environmental protective, be suitable for large-scale promotion and application; Its preparation method is simple in addition, and cost is low, is suitable for scale operation.
The theme of a first aspect of the present invention is a kind of microbial preparation for middle-and-low-yielding fields restoration of the ecosystem, it is characterized in that, component and the weight proportion thereof of described microbial preparation comprise:
A kind of preferred embodiment according to a first aspect of the invention, wherein, described genus bacillus comprise in bacillus megaterium, colloid bacillus cereus, subtilis, many viscosity genus bacillus any one or a few, preferably, comprise bacillus megaterium and colloid bacillus cereus.
Wherein, described bacillus megaterium and colloid bacillus cereus weight proportion are 1-5:1-5.
Wherein, described bacillus megaterium, more preferably, bacterial content is 10-50 hundred million cfu/g, and is Chinese agricultural microorganism bacterial classification, and preservation center is numbered ACCC.
Wherein, described colloid bacillus cereus, more preferably, bacterial content is 10-50 hundred million cfu/g, and is Chinese agricultural microorganism bacterial classification, and preservation center is numbered ACCC.
A kind of preferred embodiment according to a first aspect of the invention, wherein, the viable count of described microbial preparation is preferably >=10 hundred million/g.
A kind of preferred embodiment according to a first aspect of the invention, wherein, described microbial preparation is preferably solid powder preparation, and wherein, median size is preferably 10-100 order, is more preferably 20-80 order, is more preferably 30-70 order, is more preferably 40-60 order, as 50 orders.
A kind of preferred embodiment according to a first aspect of the invention, wherein, described distillers ' grains fermented product:
Its raw materials component and weight proportion thereof comprise:
Its preparation method comprises:
Step 1: by described raw materials component by the mixing of its weight proportion, and seal;
Step 2: within the scope of 30-35 DEG C, carries out fermentation 10-15 days, obtains described distillers ' grains fermented product.
Further, the preparation method of described plant lactobacillus liquid, Rhodopseudomonas palustris liquid and yeast saccharomyces cerevisiae liquid is: be inoculated into respectively in liquid nutrient medium by the bacterial classification of plant lactobacillus, Rhodopseudomonas palustris and yeast saccharomyces cerevisiae, static at 30 DEG C, and sealed fermenting 15 days, obtain described plant lactobacillus liquid, Rhodopseudomonas palustris liquid and yeast saccharomyces cerevisiae liquid.
Wherein, the component of described liquid nutrient medium and weight proportion thereof comprise:
Described liquid nutrient medium also comprises water.
Further, in described step 2, described fermentation is anaerobically fermenting.
The theme of a second aspect of the present invention is a kind of preparation method of microbial preparation as described in relation to the first aspect, it is characterized in that, comprise: distillers ' grains fermented product, genus bacillus, sodium humate and potassium fulvate are mixed according to its weight proportion, stirs, obtain described microbial preparation.
A kind of microbial preparation for middle-and-low-yielding fields restoration of the ecosystem of the present invention and preparation method thereof, owing to have employed technique scheme, has the following advantages and beneficial effect:
1, microbial preparation of the present invention, can improve the organic matter in soil and beneficial microorganism content fast, provides the nutrition required for beneficial microorganism growth simultaneously, wherein, by beneficial microorganism effect, the speed of P and K decomposing can be improved, effectively improve the farming level of middle-and-low-yielding fields;
2, the preparation method of microbial preparation of the present invention, can obtain the microbial preparation of high viable bacteria content, and preparation method is simple, and the kind of required bacterial classification is few, and cost is low, ecological, environmental protective, is suitable for scale operation and application.
Accompanying drawing explanation
Fig. 1 is the dehydrogenase activity histogram in cucumber soil and tomato soil under different treatment in Application Example 3 of the present invention.
Embodiment
The invention provides a kind of microbial preparation for middle-and-low-yielding fields restoration of the ecosystem, it is characterized in that, component and the weight proportion thereof of described microbial preparation comprise:
A kind of preferred embodiment according to a first aspect of the invention, wherein, described microorganism is bacillus megaterium and colloid bacillus cereus.
Wherein, described bacillus megaterium and colloid bacillus cereus weight proportion are 1-5:1-5.
Wherein, the bacterial content of described bacillus megaterium is 10-50 hundred million cfu/g, and is Chinese agricultural microorganism bacterial classification, and preservation center is numbered ACCC.
Wherein, the bacterial content of described colloid bacillus cereus is 10-50 hundred million cfu/g, and is Chinese agricultural microorganism bacterial classification, and preservation center is numbered ACCC.
A kind of preferred embodiment according to a first aspect of the invention, wherein, the viable count of described microbial preparation is >=10 hundred million/g.
A kind of preferred embodiment according to a first aspect of the invention, wherein, described microbial preparation is solid powder preparation, and wherein, median size is 40-60 order, as 50 orders.
A kind of preferred embodiment according to a first aspect of the invention, wherein, described distillers ' grains fermented product:
Its raw materials component and weight proportion thereof comprise:
Its preparation method comprises:
Step 1: by described raw materials component by the mixing of its weight proportion, and seal;
Step 2: within the scope of 30-35 DEG C, carries out fermentation 10-15 days, obtains described distillers ' grains fermented product.
Further, the preparation method of described plant lactobacillus liquid, Rhodopseudomonas palustris liquid and yeast saccharomyces cerevisiae liquid is: be inoculated into respectively in liquid nutrient medium by the bacterial classification of plant lactobacillus, Rhodopseudomonas palustris and yeast saccharomyces cerevisiae, static at 30 DEG C, and sealed fermenting 15 days, obtain described plant lactobacillus liquid, Rhodopseudomonas palustris liquid and yeast saccharomyces cerevisiae liquid.
Wherein, the component of described liquid nutrient medium and weight proportion thereof comprise:
Described liquid nutrient medium also comprises water.
Further, in described step 2, described fermentation is anaerobically fermenting.
The theme of a second aspect of the present invention is a kind of preparation method of microbial preparation as described in relation to the first aspect, it is characterized in that, comprise: distillers ' grains fermented product, genus bacillus, sodium humate and potassium fulvate are mixed according to its weight proportion, stirs, obtain described microbial preparation.
Embodiment 1
(1) a kind of making method of the microbial preparation for middle-and-low-yielding fields restoration of the ecosystem
Step 1: the bacterial classification of plant lactobacillus, Rhodopseudomonas palustris and yeast saccharomyces cerevisiae is joined respectively in the liquid nutrient medium prepared by glucose 3 parts, yeast extract paste 3 parts, dipotassium hydrogen phosphate 0.5 part, 0.5 part, sodium-chlor and suitable quantity of water, static, sealed fermenting 15 days at 30 DEG C, obtains described plant lactobacillus liquid, Rhodopseudomonas palustris liquid and yeast saccharomyces cerevisiae liquid;
Step 2: shown in following, by described raw materials component by the mixing of its weight proportion, and barrelling sealing:
Step 3: at 30 DEG C, carries out anaerobically fermenting 15 days, obtains described distillers ' grains fermented product;
Step 4: shown in following, mixes distillers ' grains fermented product, bacillus megaterium, colloid bacillus cereus, sodium humate and potassium fulvate according to its weight proportion, stirs, and obtains described microbial preparation:
(2) result
The viable count of gained microbial preparation is 2,000,000,000/g, can be used for the restoration of the ecosystem of middle-and-low-yielding fields.
Embodiment 2
(1) a kind of making method of the microbial preparation for middle-and-low-yielding fields restoration of the ecosystem
Step 1: the bacterial classification of plant lactobacillus, Rhodopseudomonas palustris and yeast saccharomyces cerevisiae is joined respectively in the liquid nutrient medium prepared by glucose 3 parts, yeast extract paste 3 parts, dipotassium hydrogen phosphate 0.5 part, 0.5 part, sodium-chlor and suitable quantity of water, static, sealed fermenting 15 days at 30 DEG C, obtains described plant lactobacillus liquid, Rhodopseudomonas palustris liquid and yeast saccharomyces cerevisiae liquid;
Step 2: shown in following, by described raw materials component by the mixing of its weight proportion, and barrelling sealing:
Step 3: at 30 DEG C, carries out anaerobically fermenting 15 days, obtains described distillers ' grains fermented product;
Step 4: shown in following, mixes distillers ' grains fermented product, bacillus megaterium, colloid bacillus cereus, sodium humate and potassium fulvate according to its weight proportion, stirs, and obtains described microbial preparation:
(2) result
The viable count of gained microbial preparation is 1,000,000,000/g, can be used for the restoration of the ecosystem of middle-and-low-yielding fields.
Application Example 3
(1) application implementation background
Microbial preparation is used in soil, micro-ecological environment in adjustable soil, improve fertilizer nutrient utilization, promote agriculture production high yield, high-quality and efficient, but at present microbial preparation is to slow down the research of the action effect in the secondary salinization of plastic vegetable greenhouse soil, acidifying and continuous cropping obstacle less, this application embodiment application soil enzymology method and microbial culture method, analyze plastic vegetable greenhouse soil microbial activities under different treatment.
(2) objective for implementation
The plantation cucumber soil that plastic vegetable greenhouse soil is corresponding and plantation tomato soil;
(3) implementation method
Three parts of identical objective for implementation are not applied fertilizer respectively, the process of traditional fertilization and microbial preparation of the present invention fertilising, detect the pH value of soil, EC, salinity total content and bacterium, fungi and Population of Actinomycetes respectively.
Wherein, in soil, bacterium, fungi and actinomycetic separation and quantitative measurement adopt dilution-plate method, namely cultivate under 28 DEG C of constant temperature and count, count results carries out statistical study after getting denary logarithm, each extent of dilution repeats 3 times, and three major types bacterium culture medium is respectively beef extract-peptone nutrient agar, Ma Dingshi substratum and Gao Shi No. 1 substratum.
(4) results and analysis
A. under different fertilization, the pH value of plastic greenhouse cucumber and tomato soil, EC value and salinity total content the results are shown in Table 1:
Table 1: the pH value of cucumber soil and tomato soil, EC value and salinity total content under different treatment
B. desaturase activity in soil
Soil deterioration index is only present in living microorganism cell interior, is the important indicator representing living microorganism in soil.As can be seen from Figure 1, without fertile all high than traditional fertilization process with cucumber desaturase activity in soil under rational application of fertilizers.Compared with traditional fertilization, under rational application of fertilizers, the activity of cucumber soil deterioration index improves 36.5%, and under rational application of fertilizers simultaneously, the desaturase activity in soil of tomato improves 66.5%.Different treatment has significant impact to desaturase activity in soil, and vegetation type does not affect significantly on soil deterioration index.
C. bacterium, fungi and Population of Actinomycetes
In soil, educable bacterium, fungi and actinomycetes are the microbiotic common counters of reflect soil.As can be seen from following table 2, there is no significant difference without cultivable bacteria and fungi in cucumber soil under useless, rational application of fertilizers and traditional fertilization, but can cultivate Population of Actinomycetes is significantly greater than traditional fertilization, adds 30%; Also be like this in tomato planting, Population of Actinomycetes can be cultivated and add 72.2% than traditional fertilization.Different treatment has no significant effect cultivable bacteria in soil and fungi, but has affect extremely significantly cultivating Population of Actinomycetes in soil, and vegetation type does not affect significantly on the bacterium in soil, fungi and Population of Actinomycetes.
Table 2: the microbe population of cucumber soil and tomato soil under different treatment
Be described in detail specific embodiments of the invention above, but it is just as example, the present invention is not restricted to specific embodiment described above.To those skilled in the art, any equivalent modifications that the present invention is carried out and substituting also all among category of the present invention.Therefore, equalization conversion done without departing from the spirit and scope of the invention and amendment, all should contain within the scope of the invention.
Claims (10)
1. for a microbial preparation for middle-and-low-yielding fields restoration of the ecosystem, it is characterized in that, component and the weight proportion thereof of described microbial preparation comprise:
2. a kind of microbial preparation for middle-and-low-yielding fields restoration of the ecosystem according to claim 1, is characterized in that, described genus bacillus comprise in bacillus megaterium, colloid bacillus cereus, subtilis, many viscosity genus bacillus any one or a few.
3. a kind of microbial preparation for middle-and-low-yielding fields restoration of the ecosystem according to claim 2, it is characterized in that, described genus bacillus is bacillus megaterium and colloid bacillus cereus, and its weight proportion is 1-5:1-5.
4. a kind of microbial preparation for middle-and-low-yielding fields restoration of the ecosystem according to claim 1, it is characterized in that, the viable count of described microbial preparation is >=10 hundred million/g.
5. a kind of microbial preparation for middle-and-low-yielding fields restoration of the ecosystem according to claim 1, it is characterized in that, described microbial preparation is solid powder preparation.
6. a kind of microbial preparation for middle-and-low-yielding fields restoration of the ecosystem according to claim 5, it is characterized in that, the median size of described microbial preparation is 40-60 order.
7. a kind of microbial preparation for middle-and-low-yielding fields restoration of the ecosystem according to claim 1, is characterized in that, described distillers ' grains fermented product:
Its raw materials component and weight proportion thereof comprise:
Its preparation method comprises:
Step 1: by described raw materials component by the mixing of its weight proportion, and seal;
Step 2: within the scope of 30-35 DEG C, carries out fermentation 10-15 days, obtains described distillers ' grains fermented product.
8. a kind of microbial preparation for middle-and-low-yielding fields restoration of the ecosystem according to claim 7, it is characterized in that, the preparation method of described plant lactobacillus liquid, Rhodopseudomonas palustris liquid and yeast saccharomyces cerevisiae liquid is: be inoculated into respectively in liquid nutrient medium by the bacterial classification of plant lactobacillus, Rhodopseudomonas palustris and yeast saccharomyces cerevisiae, static at 30 DEG C, and sealed fermenting 15 days, obtain described plant lactobacillus liquid, Rhodopseudomonas palustris liquid and yeast saccharomyces cerevisiae liquid.
9. a kind of microbial preparation for middle-and-low-yielding fields restoration of the ecosystem according to claim 8, it is characterized in that, component and the weight proportion thereof of described liquid nutrient medium comprise:
Described liquid nutrient medium also comprises water.
10. the preparation method of a microbial preparation as described in claim 1-9 any one, it is characterized in that, comprise: distillers ' grains fermented product, genus bacillus, sodium humate and potassium fulvate are mixed according to its weight proportion, stirs, obtain described microbial preparation.
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| CN108102656A (en) * | 2018-01-08 | 2018-06-01 | 贵州怡和瑞丰土地开发有限公司 | Native glue of ecology that a kind of farmland ditch is reinforced and preparation method thereof |
| CN108383580A (en) * | 2018-03-30 | 2018-08-10 | 陕西枫丹百丽生物科技有限公司 | A kind of vinasse biological organic fertilizer and its preparation method and application |
| CN109730088A (en) * | 2019-01-25 | 2019-05-10 | 江苏大学 | A kind of improvement stony desertification ground microorganism formulation of plant growth and preparation method thereof |
| CN112048307A (en) * | 2020-09-03 | 2020-12-08 | 甘肃省科学院生物研究所 | Soil improvement matrix for relieving cabbage planting continuous cropping obstacle and preparation method thereof |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109730088A (en) * | 2019-01-25 | 2019-05-10 | 江苏大学 | A kind of improvement stony desertification ground microorganism formulation of plant growth and preparation method thereof |
| CN112048307A (en) * | 2020-09-03 | 2020-12-08 | 甘肃省科学院生物研究所 | Soil improvement matrix for relieving cabbage planting continuous cropping obstacle and preparation method thereof |
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