CN103848651B - A kind of preparation method of rhodotorula glutinis bio-feritlizer and application - Google Patents
A kind of preparation method of rhodotorula glutinis bio-feritlizer and application Download PDFInfo
- Publication number
- CN103848651B CN103848651B CN201210501001.5A CN201210501001A CN103848651B CN 103848651 B CN103848651 B CN 103848651B CN 201210501001 A CN201210501001 A CN 201210501001A CN 103848651 B CN103848651 B CN 103848651B
- Authority
- CN
- China
- Prior art keywords
- rhodotorula glutinis
- application
- bio
- feritlizer
- fertilizer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Fertilizers (AREA)
Abstract
The invention provides a kind of preparation method and application of rhodotorula glutinis bio-organic fertilizer.The method obtains rhodotorula glutinis thalline for raw material to ferment, first dry thalline under 40-80 DEG C of condition, is applied to the growth of soybean or kidney bean after then being pulverized by dry thalline using the amount of application of per hectare 150-450kg as base fertilizer.In the present invention, rhodotorula glutinis has the distinguishing features such as preparation method is simple, amount of application is little as bio-feritlizer, both for rhodotorula glutinis provides a kind of novelty teabag, also can reduce the environmental pollution that causes because excessively using chemical fertilizer and affect the problems such as human health.
Description
Technical field
The present invention relates to a kind of preparation method of microorganism organic fertilizer, particularly a kind of rhodotorula glutinis is as the application of bio-feritlizer.
Background technology
Rhodotorula glutinis (Rhodotorulaglutinis), because the plurality of active ingredients such as carotenoid, lipid acid, amino acid, ubiquinone, phenylalanine ammonia lyase can be produced, receive much concern in recent years, be considered to industrialization potential bacterial strain, be widely used in preparing the fields such as animal-feed, agriculture fruit biological control of diseases, biological medicine and bioenergy.As Heilongjiang Dahuangchuan Wine Co., Ltd. discloses the method (number of patent application: 201010251117.9) that rhodotorula glutinis prepares animal-feed; Zhejiang University discloses the method (application number: 201110111499.X) that rhodotorula glutinis prepares biological preservative for oranges; Method (the number of patent application: 201010509822.4) of rhodotorula glutinis preparation (S)-hydroxypropanamide disclosed in Chinese science research institute Chengdu biology; Beijing University of Chemical Technology discloses the method (application number: 201110095230.7) etc. that rhodotorula glutinis production microbial oil prepares biofuel.But the method utilizing rhodotorula glutinis to prepare bio-organic fertilizer is not then reported.
The long-term application of current chemical fertilizer brings serious agricultural soil and pollutes (as soil acidification, harden, heavy metal contamination etc.) and agricultural-food and can not to guarantee the quality problems such as (as " melon are sweetless, and dish is not fragrant, really tasteless, and beans are poisonous ").And microbial fertilizer can be improved soil physical and chemical property, strengthen water retention capacity, improve and continue feeder capability and strengthen disease-resistant crops evil ability, therefore the application of microorganism organic fertilizer becomes a kind of and supplements and the inexorable trend of alternative chemical fertilizer.
Summary of the invention
The object of the invention is the preparation method providing rhodotorula glutinis bio-feritlizer; The object of the invention is also the novelty teabag providing rhodotorula glutinis as bio-organic fertilizer.
The present invention seeks to be achieved through the following technical solutions:
The methods for making and using same of rhodotorula glutinis fertilizer of the present invention is:
Rhodotorula glutinis fungus press after the inoculation of 5-15% pH5-6, temperature 25-35 DEG C, the condition bottom fermentation 18-48h of air flow 0.2-1.0vvm obtains using sticky red rhodotorula liquid; Then bacterium solid and liquid is separated and obtains bacterium mud in 40-80 DEG C of drying treatment acquisition bacterium block; Be 150-450kg by 1 hectare of amount of application every after acquisition bacterium block pulverization process afterwards, use in the mode of base fertilizer.
The methods for making and using same of rhodotorula glutinis fertilizer of the present invention is preferably:
Rhodotorula glutinis fungus obtains using sticky red rhodotorula liquid by after the inoculation of 10% at the condition bottom fermentation 36h of pH5.5, temperature 30 DEG C, air flow 0.8vvm; Then bacterium solid and liquid is separated and obtains bacterium mud in 50 DEG C of drying treatment acquisition bacterium blocks; Be 180kg by 1 hectare of amount of application every after acquisition bacterium block pulverization process afterwards, use in the mode of base fertilizer.
Specific embodiment is used for further illustrating but is not limited to the present invention below.
Embodiment 1 is that fermenting raw materials produces rhodotorula glutinis fertilizer application in the growth experiment of soybean with food and drink waste water
With Beijing Agricultural College student second dining room waste water for raw material substratum, Rhodotorula glutinis fungus 5% is inoculated in 5L fermentor tank, 300rpm, obtaining biomass after 1vvm fermentation 40h is the fermented liquid of 14g/L, and 60 DEG C of oven dry select soybean as experiment material after pulverizing, with vermiculite as potted plant substrate, using with the amount of application base fertilizer of per hectare 450kg, is contrast with the commercially available compound nitrogen phosphate fertilizer of equivalent, plant 7 days afterwards respectively thick the and plant height in periodic measurement footpath to test fertilizer efficiency.
Experimental result: rhodotorula glutinis utilizes food and drink waste water fermentation 18h artifact amount just can reach more than 10g/L as seen from Figure 1; As shown in Figure 2, fermentation obtains rhodotorula glutinis bio-feritlizer and is applied in the growth cycle of soybean, can reach the effect using nitrogen phosphorus composite chemical fertilizer, and comparatively nitrogen phosphorus composite chemical is fertile better at its fertilizer efficiency retentivity of plant strain growth later stage, is especially beneficial to plant footpath and slightly accumulates.
Embodiment 2 is that fermenting raw materials produces rhodotorula glutinis fertilizer application in the growth experiment of kidney bean with food and drink waste water
The acquisition of rhodotorula glutinis bacterial manure and fertilizer efficiency testing method are all with case study on implementation 1, and result of implementation is as follows:
From Fig. 4 experimental result, rhodotorula glutinis bio-feritlizer is applied in the growth cycle of kidney bean, can reach the effect using nitrogen phosphorus composite chemical fertilizer, and comparatively nitrogen phosphorus composite chemical is fertile better along with its fertilizer efficiency retentivity of prolongation of plant strain growth phase.
Following embodiment all can realize the effect of above-described embodiment 1 or embodiment 2
Embodiment 3: by common synthetic medium fermentative production rhodotorula glutinis fertilizer application in the growth experiment of soybean
Substratum composition (100mL): glucose 4g, (NH
4)
2sO
40.2g, KH
2pO
40.7g, Na
2sO
40.2g, MgSO
47H
2o0.15g, yeast powder 0.15g, pH5.5.
Embodiment 4: by common synthetic medium fermentative production rhodotorula glutinis fertilizer application in the growth experiment of kidney bean
Substratum composition is with embodiment 3.
Embodiment 5: be that fermenting raw materials produces rhodotorula glutinis fertilizer application in the acquisition of the growth experiment rhodotorula glutinis bacterial manure of kidney bean and fertilizer efficiency testing method all with case study on implementation 1 with food and drink waste water, bacterial manure amount of application changes the amount of application of per hectare 180kg into.
Accompanying drawing explanation
Fig. 1 rhodotorula glutinis utilizes food and drink waste water to ferment and accumulates biomass situation
Fig. 2 rhodotorula glutinis bio-feritlizer is on the thick impact in soybeans they grow footpath
Fig. 3 rhodotorula glutinis bio-feritlizer is on the impact of soybeans they grow plant height
The impact of Fig. 4 rhodotorula glutinis bio-feritlizer and plant height thick on kidney bean growth footpath.
Claims (2)
1. the preparation method of a rhodotorula glutinis bio-feritlizer, it is characterized in that taking rhodotorula glutinis as bacterial classification, with common synthetic medium or food and drink waste water substratum for raw material ferments, fermentation condition is inoculum size 5-15%, pH5-6, temperature 25-35 DEG C, air flow 0.2-1.0vvm, cycle 18-48h, after the using sticky red rhodotorula solid and liquid after fermentation is separated, solid bacterium mud obtains rhodotorula glutinis bio-feritlizer through 40-80 DEG C of drying treatment with after pulverizing.
2. the application of rhodotorula glutinis bio-feritlizer prepared of method as claimed in claim 1, it is characterized in that the amount of application of this fertilizer in every 1 hectare of land is 150-450kg, method of application is used with base fertilizer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210501001.5A CN103848651B (en) | 2012-11-30 | 2012-11-30 | A kind of preparation method of rhodotorula glutinis bio-feritlizer and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210501001.5A CN103848651B (en) | 2012-11-30 | 2012-11-30 | A kind of preparation method of rhodotorula glutinis bio-feritlizer and application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103848651A CN103848651A (en) | 2014-06-11 |
| CN103848651B true CN103848651B (en) | 2016-03-30 |
Family
ID=50856822
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210501001.5A Active CN103848651B (en) | 2012-11-30 | 2012-11-30 | A kind of preparation method of rhodotorula glutinis bio-feritlizer and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103848651B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105884411A (en) * | 2014-12-12 | 2016-08-24 | 北京农学院 | Rhodotorula glutinis and bacillus licheniformis liquid mixed bacterial fertilizer and application thereof |
| CN110637688A (en) * | 2019-11-05 | 2020-01-03 | 湖北煊松农业科技发展有限公司 | Planting method for applying yeast biofertilizer to agricultural fruits and vegetables |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1140159A (en) * | 1996-04-29 | 1997-01-15 | 刘文治 | Method for producing organic and inorganic mixed microbe fertilizer |
| CN1181365A (en) * | 1997-11-21 | 1998-05-13 | 董巧云 | Method for preparing animal feed or fertilizer |
| CN101195544A (en) * | 2000-09-05 | 2008-06-11 | 欧亚生物科技有限公司 | Biological fertilizer based on yeast |
| CN101255402A (en) * | 2008-04-07 | 2008-09-03 | 谭宏伟 | Thallus for producing biological fertilizer by employing cane sugar filter mud fermentation |
| CN101525253A (en) * | 2008-03-03 | 2009-09-09 | 迈德金生物技术（北京）有限公司 | Microbial fertilizer and preparation method thereof |
| CN101912032A (en) * | 2010-08-12 | 2010-12-15 | 黑龙江大荒春酒业有限公司 | Method for preparing rhodotorula glutinis feed |
| CN101912041A (en) * | 2010-08-02 | 2010-12-15 | 哈尔滨市海澳斯生物科技开发有限公司 | Preparation method of active rhodotorula glutinis powder |
| CN102060341A (en) * | 2009-11-12 | 2011-05-18 | 安琪酵母股份有限公司 | Yeast wastewater treatment method, feed additive obtained by same and feed product |
-
2012
- 2012-11-30 CN CN201210501001.5A patent/CN103848651B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1140159A (en) * | 1996-04-29 | 1997-01-15 | 刘文治 | Method for producing organic and inorganic mixed microbe fertilizer |
| CN1181365A (en) * | 1997-11-21 | 1998-05-13 | 董巧云 | Method for preparing animal feed or fertilizer |
| CN101195544A (en) * | 2000-09-05 | 2008-06-11 | 欧亚生物科技有限公司 | Biological fertilizer based on yeast |
| CN101525253A (en) * | 2008-03-03 | 2009-09-09 | 迈德金生物技术（北京）有限公司 | Microbial fertilizer and preparation method thereof |
| CN101255402A (en) * | 2008-04-07 | 2008-09-03 | 谭宏伟 | Thallus for producing biological fertilizer by employing cane sugar filter mud fermentation |
| CN102060341A (en) * | 2009-11-12 | 2011-05-18 | 安琪酵母股份有限公司 | Yeast wastewater treatment method, feed additive obtained by same and feed product |
| CN101912041A (en) * | 2010-08-02 | 2010-12-15 | 哈尔滨市海澳斯生物科技开发有限公司 | Preparation method of active rhodotorula glutinis powder |
| CN101912032A (en) * | 2010-08-12 | 2010-12-15 | 黑龙江大荒春酒业有限公司 | Method for preparing rhodotorula glutinis feed |
Non-Patent Citations (3)
| Title |
|---|
| 用味精废水浓缩液发酵生产菌体蛋白饲料;冯东勋等;《中国饲料》;19971208(第23期);第40-41页 * |
| 粘红酵母处理味精废水的研究;张志红等;《北京化工大学学报》;20070120;第34卷(第1期);第95-97页 * |
| 霉菌处理高浓度味精废水的研究;宛玲等;《上海环境科学》;19840430(第4期);第6-9页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103848651A (en) | 2014-06-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102976801B (en) | Method for producing functional microorganism organic fertilizer by using food residue | |
| CN108865951B (en) | A kind of general bacterium of camel thorn and its microbial inoculum and application | |
| CN106495826A (en) | A kind of production method of biological bacteria fruit tree drip irrigation fertilizer | |
| CN103789223A (en) | Acinetobacter calcoaceticus with free living nitrogen fixation and phosphorus and potassium dissolving capability and application of acinetobacter calcoaceticus | |
| CN102399713B (en) | Bacillus subtilis HL-1 and application thereof in respect of soil phosphate dissolving | |
| CN103992963A (en) | Bacillus megaterium and application thereof | |
| CN107858296A (en) | The preparation and application of the aspergillus niger and its microbial inoculum of one plant of phosphorus decomposing, potassium decomposing and degraded cellulose | |
| CN101913961B (en) | Weathered coal bio-organic fertilizer and production method thereof | |
| CN108676755B (en) | Microbial liquid fertilizer containing bacillus and preparation method and application thereof | |
| CN103374528B (en) | Aspergillus niger strain and application thereof | |
| CN103319217B (en) | Bio-organic fertilizer and preparation method thereof | |
| CN104928203A (en) | Bacillus mucilaginosus and high density fermenting method thereof | |
| CN103911311A (en) | Efficient phosphate-dissolving bacterium and produced bacterial agent thereof | |
| CN103320369A (en) | Composite inoculant for promoting decomposing of cellulose organic wastes, and preparation method thereof | |
| CN104232538A (en) | Efficient potassium bacterium and application thereof | |
| CN103194410A (en) | Paenibacillus mucilaginosus and method for producing compound microorganism bacterium agent by utilizing same | |
| CN106212546A (en) | One plant growth regulators | |
| CN105494715A (en) | Process for producing dark tea through inoculation method | |
| CN105948838A (en) | Compound lactic acid bacteria microbial fertilizer and preparation and application thereof | |
| CN104450573A (en) | Compound ecological microbial agent containing microbial polysaccharide and functional microbes | |
| CN110256137A (en) | A kind of seaweed microbial-bacterial fertilizer and preparation method thereof | |
| CN102815973A (en) | Preparation method of biological selenium-rich fertilizer | |
| CN103848651B (en) | A kind of preparation method of rhodotorula glutinis bio-feritlizer and application | |
| CN101787351A (en) | Special microorganism strain for processing steel slag, vinegar residue and weathered coal and preparation method thereof | |
| CN109516869A (en) | Complex function microorganism formulation and its preparation method and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20220721 Address after: 101300 in the three high tech agricultural experimental demonstration zone, Shunyi District, Beijing Patentee after: Central Xingbang (Beijing) Technology Co.,Ltd. Address before: 102206, Beijing, Huilongguan, Changping District Town, No. 7 agricultural road, Beijing University of Agriculture Patentee before: BEIJING University OF AGRICULTURE |
|
| TR01 | Transfer of patent right |