CN101912041A - Preparation method of active rhodotorula glutinis powder - Google Patents

Preparation method of active rhodotorula glutinis powder Download PDF

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Publication number
CN101912041A
CN101912041A CN2010102421204A CN201010242120A CN101912041A CN 101912041 A CN101912041 A CN 101912041A CN 2010102421204 A CN2010102421204 A CN 2010102421204A CN 201010242120 A CN201010242120 A CN 201010242120A CN 101912041 A CN101912041 A CN 101912041A
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rhodotorula glutinis
percent
weight
hours
active
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CN2010102421204A
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Chinese (zh)
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CN101912041B (en
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陈成
滕春红
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哈尔滨市海澳斯生物科技开发有限公司
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Abstract

The invention provides a preparation method of active rhodotorula glutinis powder, comprising the following steps of: firstly preparing directly throwing type leaven freeze-drying bacterium powder by taking rhodotorula glutinis as a parent strain, and inoculating 150 g of the directly throwing type leaven on every ton of fermentation mediums at 28-30 DEG C, wherein ventilation quantity is 80-95 m<3>/hour; after 24 hours, adding a nutrient source prepared from 2 percent by weight of cane sugar, 0.3 percent by weight of urea and 0.1 percent by weight of ammonium sulfate in continuous flow for 18 hours, and culturing for 48-55 hours; then taking a yeast concentrated solution by adopting a tube centrifuge for 5000 turns/minute, and adding 0.1 percent by weight of sucrose esters, 0.05 percent by weight of glycerin monostearate, 0.1 percent by weight of xylo-oligosaccharides and 1.5-5 percent by weight of dextrin according to the weight of yeast concentrated solution, sufficiently dissolving the added substances into the yeast concentrated solution, and uniformly stirring; ventilating and drying at 50 DEG C to ensure that the moisture of the additive substances is 20-25 percent, and pelleting; then continuously drying at 50 DEG C to ensure that the moisture of particles is lower than 5 percent; and then carrying out vacuum packaging.

Description

A kind of preparation method of active rhodotorula glutinis powder

(1) technical field

The present invention relates to a kind of preparation method of active rhodotorula glutinis powder, be specifically related to a kind of quick method that adopts throw type leaven, produce active rhodotorula glutinis powder, belong to bioengineering field.

(2) background technology

Rhodotorula glutinis contains multiple nutritional components such as rich in amino acid, astaxanthin, not only in the attention more and more widely of having arrived of aspects such as medicine, food, chemical industry and agricultural, and rhodotorula glutinis is good production of astaxanthin bacterium and the live single-cell ecological bait that has potentiality.Aspect aquaculture, can replace traditional bait such as unit cell algae to be used for the young open-mouthed bait of aquatic/marine animals and the complementarity bait in whole young period, strengthen aquatic livestock immunity, substitute the antibiotic use, being the good additive of ecologic breeding, is natural, healthy little ecological active bacteria formulation.

Carotenoid mainly comprises beta carotene, astaxanthin, have the prevention vascular sclerosis, suppress the tumour generation, increase functions such as host immune power and oxidation resistance, regarded as category-A nutrition pigment by international organizations such as FAO and WHO, and get permission food additives as nutrition, painted dual function more than 50 countries and regions.Beta carotene is widely used in medicines and health protection, foods and cosmetics industry.Since the toxicity problem of synthetic dyestuff, the use of many countries restriction synthetic dyestuff.Utilize rhodotorula glutinis biosynthesis beta carotene to have the following advantages: thalline is nontoxic, and contains rich in protein and vitamin; Can utilize bagasse, blackstrap etc. to cultivate, cost is low and the cycle short, fermentation control is simple, helps suitability for industrialized production, therefore, utilizes rhodotorula glutinis biosynthesis bata-carotene, will become the important source of natural beta-carotin.

Domestic enterprise of a few family few in number produces the mode of production of rhodotorula glutinis and three grades of liquid state fermentations of series of products employing or solid state fermentation, it is long to exist fermentation period, the product activity is low, complicated operation, many defectives such as easy bacteria infection, the present invention adopts the form of throw type leaven, shortened fermentation time greatly, enhance productivity and improved product purity and biologically active utilization rate of equipment and installations the time, this working of an invention, can bring huge economic benefit and social benefit, have the wide development prospect.

(3) summary of the invention

The object of the invention is to provide a kind of preparation method of active rhodotorula glutinis powder.

The object of the present invention is achieved like this: related percentage is mass ratio except that other has indicating among the present invention, and product of the present invention adopts such method to prepare:

1. the preparation of the selection of rhodotorula glutinis and culture medium

The rhodotorula glutinis (Rhodotorula glutinis) that the present invention selects is purchased in the Chinese industrial microorganism fungus kind and is contained the center, is numbered 32881, and bacterium culture medium is: 5 ° of B é brewer's worts.

2. the preparation of throw type leaven

2.1 the preparation of mother culture

Measure Rhodotorula glutinis fungus culture medium 200ml in 1 500ml triangular flask, 121 ℃ of sterilizations were cooled to 30 ℃ in 20 minutes, with oese inoculation rhodotorula glutinis bacterial strain inclined-plane one ring, cultivated 48 hours at 28-30 ℃, 150 rev/mins shaking tables, as mother culture.

2.2 produce the preparation of leavening

At first dispose 20% defatted milk emulsion, 121 ℃ of sterilizations were cooled to 28-30 ℃ in 20 minutes, and by 5% volume ratio inoculation mother culture, 28-30 ℃, 150 rev/mins shaking tables were cultivated 48 hours, detected defatted milk emulsion rhodotorula glutinis viable count 〉=10 9Individual/ml, treat as and be fermenting-ripening, if viable count<10 9Individual/ml, continue to cultivate, until reaching 10 9Individual/ml, finish to cultivate, freeze-drying is stand-by.

2.3 the preparation of throw type leaven

Under aseptic condition, import the production leavening of maturation in the glass ampoule, liquid level is lower than 0.8-1cm, puts into-30 ℃ of refrigerator-freezer quick-frozens after adding a cover bottle stopper, after freezing glass ampoule is taken advantage of dress with pallet, put into freeze dryer and carry out freeze drying, make throw type leaven.

3. the preparation of active rhodotorula glutinis powder

3.1 the preparation of fermentation medium

Glucose 2-5%

Sucrose 3%

Magnesium sulfate 0.15%

Yeast extract (moisture<30%) 0.2%

Dipotassium hydrogen phosphate 0.5%

Urea 0.2%

Ammonium sulfate 0.1%

Potassium nitrate 0.1%

PH value 4-5

3.2 rhodotorula glutinis fermented and cultured

Inoculum concentration: fermentation medium inoculation 150g throw type leaven per ton.

Condition of culture: 28-30 ℃, ventilation 80-95m 3/ hour, incubation time: 48-55 hour.

The nutrition source and course adds condition: press fermentation medium weight and add nutrient source, comprising: sucrose 2%, and urea 0.3%, ammonium sulfate 0.1%, with the solution that is mixed with 30% after three kinds of raw materials mixing, Continuous Flow Ensure Liquid source after fermenting 24 hours added in 18 hours.

3.3 rhodotorula glutinis concentrates

Adopt tube centrifuge, 5000 rev/mins, get the yeast concentrate, add 0.1% sucrose ester by yeast concentrate weight, 0.05% glycerin monostearate, 0.1% xylo-oligosaccharide, the 1.5-5% dextrin adds materials with these and fully is dissolved in the yeast concentrate and stirs.

3.4 rhodotorula glutinis finished product preparation

50 ℃ of aeration-dryings make its moisture at 20-25%, carry out granulation, and 50 ℃ are continued drying then, make pellet moisture be lower than 5%, carry out vacuum packaging then.

(4) specific embodiment

For a more detailed description below in conjunction with specific embodiment to the present invention:

Embodiment one:

1. the preparation of the selection of rhodotorula glutinis and culture medium

The rhodotorula glutinis (Rhodotorula glutinis) that the present invention selects is purchased in the Chinese industrial microorganism fungus kind and is contained the center, is numbered 32881, and bacterium culture medium is: 5 ° of B é brewer's worts.

2. the preparation of throw type leaven

2.1 the preparation of mother culture

Measure Rhodotorula glutinis fungus culture medium 200ml in 1 500ml triangular flask, 121 ℃ of sterilizations were cooled to 30 ℃ in 20 minutes, with oese inoculation rhodotorula glutinis bacterial strain inclined-plane one ring, cultivated 48 hours at 28-30 ℃, 150 rev/mins shaking tables, as mother culture.

2.2 produce the preparation of leavening

At first dispose 20% defatted milk emulsion, 121 ℃ of sterilizations were cooled to 28-30 ℃ in 20 minutes, and by 5% volume ratio inoculation mother culture, 28-30 ℃, 150 rev/mins shaking tables were cultivated 48 hours, detected defatted milk emulsion rhodotorula glutinis viable count 〉=10 9Individual/ml, treat as and be fermenting-ripening, if viable count<10 9Individual/ml, continue to cultivate, until reaching 10 9Individual/ml, finish to cultivate, freeze-drying is stand-by.

2.3 the preparation of throw type leaven

Under aseptic condition, import the production leavening of maturation in the glass ampoule, liquid level is lower than 0.8-1cm, puts into-30 ℃ of refrigerator-freezer quick-frozens after adding a cover bottle stopper, after freezing glass ampoule is taken advantage of dress with pallet, put into freeze dryer and carry out freeze drying, make throw type leaven.

3. the preparation of active rhodotorula glutinis powder

3.1 the preparation of fermentation medium

Glucose 2-5%

Sucrose 3%

Magnesium sulfate 0.15%

Yeast extract (moisture<30%) 0.2%

Dipotassium hydrogen phosphate 0.5%

Urea 0.2%

Ammonium sulfate 0.1%

Potassium nitrate 0.1%

PH value 4-5

3.2 rhodotorula glutinis fermented and cultured

Inoculum concentration: fermentation medium inoculation 150g throw type leaven per ton.

Condition of culture: 28-30 ℃, ventilation 80-95m 3/ hour, incubation time: 48-55 hour.

The nutrition source and course adds condition: press fermentation medium weight and add nutrient source, comprising: sucrose 2%, and urea 0.3%, ammonium sulfate 0.1%, with the solution that is mixed with 30% after three kinds of raw materials mixing, Continuous Flow Ensure Liquid source after fermenting 24 hours added in 18 hours.

3.3 rhodotorula glutinis concentrates

Adopt tube centrifuge, 5000 rev/mins, get the yeast concentrate, add 0.1% sucrose ester by yeast concentrate weight, 0.05% glycerin monostearate, 0.1% xylo-oligosaccharide, 1.5% dextrin adds materials with these and fully is dissolved in the yeast concentrate and stirs.

3.4 rhodotorula glutinis finished product preparation

50 ℃ of aeration-dryings make its moisture at 20-25%, carry out granulation, and 50 ℃ are continued drying then, make pellet moisture be lower than 5%, carry out vacuum packaging then.

Embodiment two:

1. the preparation of the selection of rhodotorula glutinis and culture medium

The rhodotorula glutinis (Rhodotorula glutinis) that the present invention selects is purchased in the Chinese industrial microorganism fungus kind and is contained the center, is numbered 32881, and bacterium culture medium is: 5 ° of B é brewer's worts.

2. the preparation of throw type leaven

2.1 the preparation of mother culture

Measure Rhodotorula glutinis fungus culture medium 200ml in 1 500ml triangular flask, 121 ℃ of sterilizations were cooled to 30 ℃ in 20 minutes, with oese inoculation rhodotorula glutinis bacterial strain inclined-plane one ring, cultivated 48 hours at 28-30 ℃, 150 rev/mins shaking tables, as mother culture.

2.2 produce the preparation of leavening

At first dispose 20% defatted milk emulsion, 121 ℃ of sterilizations were cooled to 28-30 ℃ in 20 minutes, and by 5% volume ratio inoculation mother culture, 28-30 ℃, 150 rev/mins shaking tables were cultivated 48 hours, detected defatted milk emulsion rhodotorula glutinis viable count 〉=10 9Individual/ml, treat as and be fermenting-ripening, if viable count<10 9Individual/ml, continue to cultivate, until reaching 10 9Individual/ml, finish to cultivate, freeze-drying is stand-by.

2.3 the preparation of throw type leaven

Under aseptic condition, import the production leavening of maturation in the glass ampoule, liquid level is lower than 0.8-1cm, puts into-30 ℃ of refrigerator-freezer quick-frozens after adding a cover bottle stopper, after freezing glass ampoule is taken advantage of dress with pallet, put into freeze dryer and carry out freeze drying, make throw type leaven.

3. the preparation of active rhodotorula glutinis powder

3.1 the preparation of fermentation medium

Glucose 2-5%

Sucrose 3%

Magnesium sulfate 0.15%

Yeast extract (moisture<30%) 0.2%

Dipotassium hydrogen phosphate 0.5%

Urea 0.2%

Ammonium sulfate 0.1%

Potassium nitrate 0.1%

PH value 4-5

3.2 rhodotorula glutinis fermented and cultured

Inoculum concentration: fermentation medium inoculation 150g throw type leaven per ton.

Condition of culture: 28-30 ℃, ventilation 80-95m 3/ hour, incubation time: 48-55 hour.

The nutrition source and course adds condition: press fermentation medium weight and add nutrient source, comprising: sucrose 2%, and urea 0.3%, ammonium sulfate 0.1%, with the solution that is mixed with 30% after three kinds of raw materials mixing, Continuous Flow Ensure Liquid source after fermenting 24 hours added in 18 hours.

3.3 rhodotorula glutinis concentrates

Adopt tube centrifuge, 5000 rev/mins, get the yeast concentrate, add 0.1% sucrose ester by yeast concentrate weight, 0.05% glycerin monostearate, 0.1% xylo-oligosaccharide, 5% dextrin adds materials with these and fully is dissolved in the yeast concentrate and stirs.

3.4 rhodotorula glutinis finished product preparation

50 ℃ of aeration-dryings make its moisture 2025%, carry out granulation, and 50 ℃ are continued drying then, make pellet moisture be lower than 5%, carry out vacuum packaging then.

Claims (5)

1. the preparation method of an active rhodotorula glutinis powder is characterized in that: fermentation medium inoculation 150g throw type leaven per ton, 28-30 ℃, ventilation 80-95m 3/ hour, through overcurrent Ensure Liquid source, incubation time 48-55 hour, adopt tube centrifuge then, 5000 rev/mins, get the yeast concentrate, add 0.1% sucrose ester by yeast concentrate weight, 0.05% glycerin monostearate, 0.1% xylo-oligosaccharide, the 1.5-5% dextrin adds materials with these and fully is dissolved in the yeast concentrate and stirs, and 50 ℃ of aeration-dryings make its moisture at 20-25%, carry out granulation, 50 ℃ are continued drying then, make pellet moisture be lower than 5%, carry out vacuum packaging then.
2. the preparation method of a kind of active rhodotorula glutinis powder according to claim 1, it is characterized in that: fermentation medium adopts this quadrat method preparation: glucose 2-5%, sucrose 3%, magnesium sulfate 0.15%, yeast extract (moisture<30%) 0.2%, dipotassium hydrogen phosphate 0.5%, urea 0.2%, ammonium sulfate 0.1%, potassium nitrate 0.1%, pH value 4-5.
3. the preparation method of a kind of active rhodotorula glutinis powder according to claim 1, it is characterized in that: throw type leaven adopts this quadrat method preparation: measure 5 ° of B é brewer's wort 200ml in 1 500ml triangular flask, 121 ℃ of sterilizations were cooled to 30 ℃ in 20 minutes, with oese inoculation rhodotorula glutinis bacterial strain inclined-plane one ring, cultivated 48 hours at 28-30 ℃, 150 rev/mins shaking tables, as mother culture; Dispose 20% defatted milk emulsion then, 121 ℃ of sterilizations were cooled to 28-30 ℃ in 20 minutes, and by 5% volume ratio inoculation mother culture, 28-30 ℃, 150 rev/mins shaking tables were cultivated 48 hours, detected defatted milk emulsion rhodotorula glutinis viable count 〉=10 9Individual/ml, treat as and be fermenting-ripening, if viable count<10 9Individual/ml, continue to cultivate, until reaching 10 9Individual/ml, finish to cultivate, freeze-drying is stand-by; Under aseptic condition, import the production leavening of maturation in the glass ampoule, liquid level is lower than 0.8-1cm, puts into-30 ℃ of refrigerator-freezer quick-frozens after adding a cover bottle stopper, after freezing glass ampoule is taken advantage of dress with pallet, put into freeze dryer and carry out freeze drying, make throw type leaven.
4. the preparation method of a kind of active rhodotorula glutinis powder according to claim 1, it is characterized in that: this quadrat method preparation is adopted in stream Ensure Liquid source: press fermentation medium weight and add nutrient source, comprise: sucrose 2%, urea 0.3%, ammonium sulfate 0.1%, solution with being mixed with 30% after three kinds of raw materials mixing carries out stream and adds.
5. the preparation method of a kind of active rhodotorula glutinis powder according to claim 4 is characterized in that: Continuous Flow Ensure Liquid source after fermenting 24 hours added in 18 hours.
CN2010102421204A 2010-08-02 2010-08-02 Preparation method of active rhodotorula glutinis powder CN101912041B (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102119767A (en) * 2011-01-25 2011-07-13 王立新 Production technology of eggs with high carotene content
CN103848651A (en) * 2012-11-30 2014-06-11 北京农学院 Preparation method and applications of rhodotorula glutinis biofertilizer
CN106690124A (en) * 2016-12-15 2017-05-24 湖北工业大学 Method for producing vinasse flavored striped tuna containing rich beta-carotene by utilizing vinasse

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1058989A (en) * 1991-08-28 1992-02-26 中国生物工程开发中心宜昌食用酵母基地 Process for refractory alcohol active dried yeast
CN101173222A (en) * 2007-10-15 2008-05-07 四川循环科技有限公司 Method for producing active dry yeast for sweet potato produced amylolysis ferment
CN101570735A (en) * 2009-06-12 2009-11-04 河南工业大学 Method for producing flour yeast by using corn flour as raw material
CN101575578A (en) * 2008-05-06 2009-11-11 安琪酵母股份有限公司 Saccharomyces cerevisiae, dry yeast rich in reduced glutathione and preparation method thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1058989A (en) * 1991-08-28 1992-02-26 中国生物工程开发中心宜昌食用酵母基地 Process for refractory alcohol active dried yeast
CN101173222A (en) * 2007-10-15 2008-05-07 四川循环科技有限公司 Method for producing active dry yeast for sweet potato produced amylolysis ferment
CN101575578A (en) * 2008-05-06 2009-11-11 安琪酵母股份有限公司 Saccharomyces cerevisiae, dry yeast rich in reduced glutathione and preparation method thereof
CN101570735A (en) * 2009-06-12 2009-11-04 河南工业大学 Method for producing flour yeast by using corn flour as raw material

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
《食品工业科技》 20071231 张辉等 鲁氏酵母和球拟酵母活性干酵母的制备研究 64-66 1-5 第28卷, 第08期 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102119767A (en) * 2011-01-25 2011-07-13 王立新 Production technology of eggs with high carotene content
CN103848651A (en) * 2012-11-30 2014-06-11 北京农学院 Preparation method and applications of rhodotorula glutinis biofertilizer
CN103848651B (en) * 2012-11-30 2016-03-30 北京农学院 A kind of preparation method of rhodotorula glutinis bio-feritlizer and application
CN106690124A (en) * 2016-12-15 2017-05-24 湖北工业大学 Method for producing vinasse flavored striped tuna containing rich beta-carotene by utilizing vinasse

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Inventor after: Li Xiaomei

Inventor after: Chen Cheng

Inventor after: Jiang Fan

Inventor after: Teng Chunhong

Inventor before: Chen Cheng

Inventor before: Teng Chunhong

COR Change of bibliographic data

Free format text: CORRECT: INVENTOR; FROM: CHEN CHENG TENG CHUNHONG TO: LI XIAOMEI CHEN CHENG JIANG FAN TENG CHUNHONG

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Effective date of registration: 20160525

Address after: 841000, 58 Korla Road, the Xinjiang Uygur Autonomous Region economic and Technological Development Zone

Patentee after: Xinjiang Kerry Food Technology Co., Ltd.

Address before: 150030 Heilongjiang Province, Harbin city Xiangfang District Gongbin road Wood Street No. 59

Patentee before: Harbin Haiaosi Biotechnology Development Co., Ltd.