CN107488710A - 一种Cas蛋白的用途及靶标核酸分子的检测方法和试剂盒 - Google Patents
一种Cas蛋白的用途及靶标核酸分子的检测方法和试剂盒 Download PDFInfo
- Publication number
- CN107488710A CN107488710A CN201710573752.0A CN201710573752A CN107488710A CN 107488710 A CN107488710 A CN 107488710A CN 201710573752 A CN201710573752 A CN 201710573752A CN 107488710 A CN107488710 A CN 107488710A
- Authority
- CN
- China
- Prior art keywords
- dna
- cas12a
- target
- nucleic acids
- target nucleic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000001514 detection method Methods 0.000 title claims abstract description 78
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 56
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 55
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 55
- 108020004414 DNA Proteins 0.000 claims abstract description 232
- 108700004991 Cas12a Proteins 0.000 claims abstract description 112
- 238000006243 chemical reaction Methods 0.000 claims abstract description 54
- 238000000034 method Methods 0.000 claims abstract description 53
- 239000000523 sample Substances 0.000 claims abstract description 38
- 108020005004 Guide RNA Proteins 0.000 claims abstract description 18
- 230000003321 amplification Effects 0.000 claims abstract description 17
- 238000003199 nucleic acid amplification method Methods 0.000 claims abstract description 17
- 108020004711 Nucleic Acid Probes Proteins 0.000 claims abstract description 15
- 239000002853 nucleic acid probe Substances 0.000 claims abstract description 15
- 206010064571 Gene mutation Diseases 0.000 claims abstract description 4
- 244000000010 microbial pathogen Species 0.000 claims abstract description 4
- 102000053602 DNA Human genes 0.000 claims description 74
- 108020004682 Single-Stranded DNA Proteins 0.000 claims description 56
- 238000005520 cutting process Methods 0.000 claims description 49
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims description 39
- 230000000694 effects Effects 0.000 claims description 24
- 239000007853 buffer solution Substances 0.000 claims description 23
- 101000860092 Francisella tularensis subsp. novicida (strain U112) CRISPR-associated endonuclease Cas12a Proteins 0.000 claims description 18
- 108090000623 proteins and genes Proteins 0.000 claims description 12
- 150000001875 compounds Chemical class 0.000 claims description 9
- -1 Cas albumen Proteins 0.000 claims description 8
- 102000004169 proteins and genes Human genes 0.000 claims description 6
- 238000010791 quenching Methods 0.000 claims description 3
- 230000000171 quenching effect Effects 0.000 claims description 3
- 201000010099 disease Diseases 0.000 claims description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 2
- 239000007850 fluorescent dye Substances 0.000 claims description 2
- 238000001215 fluorescent labelling Methods 0.000 claims description 2
- 238000001917 fluorescence detection Methods 0.000 claims 2
- 230000007018 DNA scission Effects 0.000 claims 1
- 230000035945 sensitivity Effects 0.000 abstract description 11
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract description 7
- 238000005516 engineering process Methods 0.000 abstract description 5
- 238000004458 analytical method Methods 0.000 abstract description 3
- 238000003556 assay Methods 0.000 abstract description 3
- 238000012360 testing method Methods 0.000 description 33
- 230000004044 response Effects 0.000 description 22
- 108091034117 Oligonucleotide Proteins 0.000 description 21
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 21
- 239000003153 chemical reaction reagent Substances 0.000 description 19
- 230000035772 mutation Effects 0.000 description 17
- 230000001186 cumulative effect Effects 0.000 description 15
- 102100039087 Peptidyl-alpha-hydroxyglycine alpha-amidating lyase Human genes 0.000 description 12
- 238000000137 annealing Methods 0.000 description 11
- 238000013518 transcription Methods 0.000 description 11
- 230000035897 transcription Effects 0.000 description 11
- 108091033409 CRISPR Proteins 0.000 description 10
- 238000004925 denaturation Methods 0.000 description 10
- 230000036425 denaturation Effects 0.000 description 10
- 102000004190 Enzymes Human genes 0.000 description 9
- 108090000790 Enzymes Proteins 0.000 description 9
- 229940088598 enzyme Drugs 0.000 description 9
- 239000000243 solution Substances 0.000 description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 9
- 201000005569 Gout Diseases 0.000 description 8
- 238000003776 cleavage reaction Methods 0.000 description 8
- 230000002779 inactivation Effects 0.000 description 8
- 238000011160 research Methods 0.000 description 8
- 230000015572 biosynthetic process Effects 0.000 description 7
- 230000004087 circulation Effects 0.000 description 7
- 238000002474 experimental method Methods 0.000 description 7
- 244000005700 microbiome Species 0.000 description 7
- 239000000872 buffer Substances 0.000 description 6
- 230000007423 decrease Effects 0.000 description 6
- 230000007017 scission Effects 0.000 description 6
- 239000000758 substrate Substances 0.000 description 6
- 238000003786 synthesis reaction Methods 0.000 description 6
- 238000010354 CRISPR gene editing Methods 0.000 description 5
- 241000660147 Escherichia coli str. K-12 substr. MG1655 Species 0.000 description 5
- 244000052769 pathogen Species 0.000 description 5
- 230000001717 pathogenic effect Effects 0.000 description 5
- 239000003161 ribonuclease inhibitor Substances 0.000 description 5
- 241000588724 Escherichia coli Species 0.000 description 4
- 238000012408 PCR amplification Methods 0.000 description 4
- 239000012634 fragment Substances 0.000 description 4
- 238000009396 hybridization Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 239000000178 monomer Substances 0.000 description 4
- 108700025694 p53 Genes Proteins 0.000 description 4
- 230000009467 reduction Effects 0.000 description 4
- 238000012163 sequencing technique Methods 0.000 description 4
- 239000002253 acid Substances 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 210000000981 epithelium Anatomy 0.000 description 3
- 230000006870 function Effects 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 210000004400 mucous membrane Anatomy 0.000 description 3
- 239000013612 plasmid Substances 0.000 description 3
- 239000013641 positive control Substances 0.000 description 3
- 238000002331 protein detection Methods 0.000 description 3
- 238000003753 real-time PCR Methods 0.000 description 3
- 241000894007 species Species 0.000 description 3
- 230000008685 targeting Effects 0.000 description 3
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 108010042407 Endonucleases Proteins 0.000 description 2
- 102000004533 Endonucleases Human genes 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- 201000011510 cancer Diseases 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 239000004202 carbamide Substances 0.000 description 2
- 230000003197 catalytic effect Effects 0.000 description 2
- 238000012790 confirmation Methods 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 238000001502 gel electrophoresis Methods 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 101150013736 gyrB gene Proteins 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 238000011901 isothermal amplification Methods 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 230000001404 mediated effect Effects 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 239000002773 nucleotide Substances 0.000 description 2
- 125000003729 nucleotide group Chemical group 0.000 description 2
- 101150012629 parE gene Proteins 0.000 description 2
- 108091008146 restriction endonucleases Proteins 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- 239000012137 tryptone Substances 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- 108010011619 6-Phytase Proteins 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 241000193412 Alicyclobacillus acidoterrestris Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 108091079001 CRISPR RNA Proteins 0.000 description 1
- 238000010356 CRISPR-Cas9 genome editing Methods 0.000 description 1
- 102220613830 Casein kinase II subunit alpha 3_D1255A_mutation Human genes 0.000 description 1
- 102220613443 Casein kinase II subunit alpha 3_E1006A_mutation Human genes 0.000 description 1
- 102220613807 Casein kinase II subunit alpha 3_R1218A_mutation Human genes 0.000 description 1
- 108020004394 Complementary RNA Proteins 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 108010008532 Deoxyribonuclease I Proteins 0.000 description 1
- 102000007260 Deoxyribonuclease I Human genes 0.000 description 1
- 208000028782 Hereditary disease Diseases 0.000 description 1
- 208000026350 Inborn Genetic disease Diseases 0.000 description 1
- 208000024556 Mendelian disease Diseases 0.000 description 1
- 108020004485 Nonsense Codon Proteins 0.000 description 1
- 102000018120 Recombinases Human genes 0.000 description 1
- 108010091086 Recombinases Proteins 0.000 description 1
- 102000006382 Ribonucleases Human genes 0.000 description 1
- 108010083644 Ribonucleases Proteins 0.000 description 1
- 101710137500 T7 RNA polymerase Proteins 0.000 description 1
- 102100026260 Titin Human genes 0.000 description 1
- 241000907316 Zika virus Species 0.000 description 1
- 208000020329 Zika virus infectious disease Diseases 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 125000000539 amino acid group Chemical group 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 230000008827 biological function Effects 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 238000002038 chemiluminescence detection Methods 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 239000003184 complementary RNA Substances 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000002532 enzyme inhibitor Substances 0.000 description 1
- 239000000686 essence Substances 0.000 description 1
- 238000002509 fluorescent in situ hybridization Methods 0.000 description 1
- 235000021393 food security Nutrition 0.000 description 1
- 238000013467 fragmentation Methods 0.000 description 1
- 238000006062 fragmentation reaction Methods 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000007901 in situ hybridization Methods 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 238000007834 ligase chain reaction Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000003068 molecular probe Substances 0.000 description 1
- 230000037434 nonsense mutation Effects 0.000 description 1
- 229940085127 phytase Drugs 0.000 description 1
- 102000054765 polymorphisms of proteins Human genes 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- NLJUOWMZPAVXHU-UHFFFAOYSA-N prop-2-enamide;urea Chemical compound NC(N)=O.NC(=O)C=C NLJUOWMZPAVXHU-UHFFFAOYSA-N 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 230000003252 repetitive effect Effects 0.000 description 1
- 238000010839 reverse transcription Methods 0.000 description 1
- 108091092562 ribozyme Proteins 0.000 description 1
- 238000005096 rolling process Methods 0.000 description 1
- 238000011896 sensitive detection Methods 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- NMEHNETUFHBYEG-IHKSMFQHSA-N tttn Chemical group C([C@@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC=1NC=NC=1)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](C)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C(C)C)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N1[C@@H](CCC1)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCSC)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)[C@@H](NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](N)[C@@H](C)O)[C@@H](C)O)C1=CC=CC=C1 NMEHNETUFHBYEG-IHKSMFQHSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6823—Release of bound markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
- C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Pathology (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Hospice & Palliative Care (AREA)
- Oncology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
Claims (10)
Priority Applications (23)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201710573752.0A CN107488710B (zh) | 2017-07-14 | 2017-07-14 | 一种Cas蛋白的用途及靶标核酸分子的检测方法和试剂盒 |
CN202011092324.4A CN112501254B (zh) | 2017-07-14 | 2017-07-14 | 一种Cas蛋白的用途及靶标核酸分子的检测方法和试剂盒 |
AU2018299445A AU2018299445B2 (en) | 2017-07-14 | 2018-04-12 | Application of Cas protein, method for detecting target nucleic acid molecule and kit |
EA202090290A EA202090290A1 (ru) | 2017-07-14 | 2018-04-12 | ПРИМЕНЕНИЕ БЕЛКА Cas, СПОСОБ ДЛЯ ОБНАРУЖЕНИЯ ЦЕЛЕВОЙ МОЛЕКУЛЫ НУКЛЕИНОВОЙ КИСЛОТЫ И НАБОР |
PCT/CN2018/082769 WO2019011022A1 (zh) | 2017-07-14 | 2018-04-12 | 一种Cas蛋白的用途及靶标核酸分子的检测方法和试剂盒 |
CN202410308071.1A CN118207297A (zh) | 2017-07-14 | 2018-04-12 | 一种Cas蛋白的用途及靶标核酸分子的检测方法和试剂盒 |
EP18832456.0A EP3653722A4 (en) | 2017-07-14 | 2018-04-12 | APPLICATION OF A CAS PROTEIN, METHOD OF DETECTION OF A TARGET NUCLEIC ACID MOLECULE AND KIT |
BR112020000809-5A BR112020000809A2 (pt) | 2017-07-14 | 2018-04-12 | uso da proteína cas, método para detecção de molécula de ácido nucleico, e kit |
NZ760987A NZ760987B2 (en) | 2018-04-12 | Application of cas protein, method for detecting target nucleic acid molecule and kit | |
SG11202000336RA SG11202000336RA (en) | 2017-07-14 | 2018-04-12 | Application of cas protein, method for detecting target nucleic acid molecule and kit |
CN201880046701.5A CN111094588B (zh) | 2017-07-14 | 2018-04-12 | 一种Cas蛋白的用途及靶标核酸分子的检测方法和试剂盒 |
US16/631,157 US20230002811A1 (en) | 2017-07-14 | 2018-04-12 | Application of cas protein, method for detecting target nucleic acid molecule and kit |
CA3069788A CA3069788A1 (en) | 2017-07-14 | 2018-04-12 | Use of cas protein, method for detecting target nucleic acid molecule, and kit |
KR1020207003220A KR20200035956A (ko) | 2017-07-14 | 2018-04-12 | Cas 단백질의 용도, 표적 핵산 분자를 검출하는 방법, 및 키트 |
MX2020000481A MX2020000481A (es) | 2017-07-14 | 2018-04-12 | Uso de proteína cas, método para detectar moléculas de ácido nucleico objetivo y kit. |
MA049579A MA49579A (fr) | 2017-07-14 | 2018-04-12 | Application d'une protéine cas, procédé de détection d'une molécule d'acide nucléique cible et kit |
JP2020523474A JP2020530779A (ja) | 2017-07-14 | 2018-04-12 | Casタンパク質の使用、標的核酸分子の検出方法、及びキット |
IL272005A IL272005A (en) | 2017-07-14 | 2020-01-13 | Application of protein CAS, a method for detecting the target of a nucleic acid molecule and its value |
PH12020500103A PH12020500103A1 (en) | 2017-07-14 | 2020-01-14 | Use of cas protein, method for detecting target nucleic acid molecule, and kit |
ZA2020/00682A ZA202000682B (en) | 2017-07-14 | 2020-01-31 | Application of cas protein, method for detecting target nucleic acid molecule and kit |
US17/224,541 US11584955B2 (en) | 2017-07-14 | 2021-04-07 | Application of Cas protein, method for detecting target nucleic acid molecule and kit |
US18/085,121 US20230131421A1 (en) | 2017-07-14 | 2022-12-20 | Application of cas protein, method for detecting target nucleic acid molecule and kit |
JP2023065743A JP2023080282A (ja) | 2017-07-14 | 2023-04-13 | Casタンパク質の使用、標的核酸分子の検出方法、及びキット |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201710573752.0A CN107488710B (zh) | 2017-07-14 | 2017-07-14 | 一种Cas蛋白的用途及靶标核酸分子的检测方法和试剂盒 |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202011092324.4A Division CN112501254B (zh) | 2017-07-14 | 2017-07-14 | 一种Cas蛋白的用途及靶标核酸分子的检测方法和试剂盒 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN107488710A true CN107488710A (zh) | 2017-12-19 |
CN107488710B CN107488710B (zh) | 2020-09-22 |
Family
ID=60643750
Family Applications (4)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201710573752.0A Active CN107488710B (zh) | 2017-07-14 | 2017-07-14 | 一种Cas蛋白的用途及靶标核酸分子的检测方法和试剂盒 |
CN202011092324.4A Active CN112501254B (zh) | 2017-07-14 | 2017-07-14 | 一种Cas蛋白的用途及靶标核酸分子的检测方法和试剂盒 |
CN202410308071.1A Pending CN118207297A (zh) | 2017-07-14 | 2018-04-12 | 一种Cas蛋白的用途及靶标核酸分子的检测方法和试剂盒 |
CN201880046701.5A Active CN111094588B (zh) | 2017-07-14 | 2018-04-12 | 一种Cas蛋白的用途及靶标核酸分子的检测方法和试剂盒 |
Family Applications After (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202011092324.4A Active CN112501254B (zh) | 2017-07-14 | 2017-07-14 | 一种Cas蛋白的用途及靶标核酸分子的检测方法和试剂盒 |
CN202410308071.1A Pending CN118207297A (zh) | 2017-07-14 | 2018-04-12 | 一种Cas蛋白的用途及靶标核酸分子的检测方法和试剂盒 |
CN201880046701.5A Active CN111094588B (zh) | 2017-07-14 | 2018-04-12 | 一种Cas蛋白的用途及靶标核酸分子的检测方法和试剂盒 |
Country Status (16)
Country | Link |
---|---|
US (2) | US20230002811A1 (zh) |
EP (1) | EP3653722A4 (zh) |
JP (2) | JP2020530779A (zh) |
KR (1) | KR20200035956A (zh) |
CN (4) | CN107488710B (zh) |
AU (1) | AU2018299445B2 (zh) |
BR (1) | BR112020000809A2 (zh) |
CA (1) | CA3069788A1 (zh) |
EA (1) | EA202090290A1 (zh) |
IL (1) | IL272005A (zh) |
MA (1) | MA49579A (zh) |
MX (1) | MX2020000481A (zh) |
PH (1) | PH12020500103A1 (zh) |
SG (1) | SG11202000336RA (zh) |
WO (1) | WO2019011022A1 (zh) |
ZA (1) | ZA202000682B (zh) |
Cited By (70)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108220400A (zh) * | 2018-01-02 | 2018-06-29 | 武汉启丰生物技术有限公司 | 一种基于CRISPR-Cas10核酸蛋白复合物的快捷核酸检测定量法 |
CN108588182A (zh) * | 2018-04-13 | 2018-09-28 | 中国科学院深圳先进技术研究院 | 基于crispr-链取代的等温扩增及检测技术 |
CN108796036A (zh) * | 2018-04-03 | 2018-11-13 | 上海交通大学 | 基于原核Argonaute蛋白的核酸检测方法及其应用 |
WO2019011022A1 (zh) * | 2017-07-14 | 2019-01-17 | 上海吐露港生物科技有限公司 | 一种Cas蛋白的用途及靶标核酸分子的检测方法和试剂盒 |
CN109251963A (zh) * | 2018-11-12 | 2019-01-22 | 复旦大学 | 恒温检测细胞培养液中支原体污染的方法及试剂盒 |
US10253365B1 (en) | 2017-11-22 | 2019-04-09 | The Regents Of The University Of California | Type V CRISPR/Cas effector proteins for cleaving ssDNAs and detecting target DNAs |
CN109628564A (zh) * | 2019-02-25 | 2019-04-16 | 北京市理化分析测试中心 | 一种用于检测snp多态性的引物组和利用引物组检测snp多态性的方法 |
CN109666662A (zh) * | 2018-12-12 | 2019-04-23 | 广州普世利华科技有限公司 | 新型ScCas12a在核酸检测方面的应用 |
CN109837328A (zh) * | 2018-09-20 | 2019-06-04 | 中国科学院动物研究所 | 核酸检测方法 |
CN109929949A (zh) * | 2019-03-14 | 2019-06-25 | 广州医科大学附属肿瘤医院 | 基于CRISPR-Cas12a和G四链体-血红素的EBV的检测方法及应用 |
CN110029194A (zh) * | 2019-04-24 | 2019-07-19 | 安邦(厦门)生物科技有限公司 | 基于CRISPR-Cas基因编辑技术的连续荧光监测检测方法及装置 |
CN110117818A (zh) * | 2019-04-16 | 2019-08-13 | 天津大学 | 检测单基因突变的crispr高通量生物芯片 |
CN110157821A (zh) * | 2019-05-06 | 2019-08-23 | 浙江大学 | 一种用于人葡萄球菌检测的试剂盒 |
CN110195102A (zh) * | 2019-04-30 | 2019-09-03 | 广州普世利华科技有限公司 | 一种β地中海贫血基因分型方法 |
CN110241238A (zh) * | 2019-06-24 | 2019-09-17 | 浙江大学 | 一种用于化脓链球菌检测的试剂盒 |
CN110257556A (zh) * | 2019-04-30 | 2019-09-20 | 广州普世利华科技有限公司 | 一种性传播疾病致病病原的核酸检测试剂盒 |
CN110317890A (zh) * | 2019-06-24 | 2019-10-11 | 浙江大学 | 一种用于粪肠球菌检测的试剂盒 |
CN110331219A (zh) * | 2019-06-24 | 2019-10-15 | 浙江大学 | 一种用于铜绿假单胞菌检测的试剂盒 |
CN110358848A (zh) * | 2019-06-24 | 2019-10-22 | 浙江大学 | 一种用于屎肠球菌检测的试剂盒 |
CN110551812A (zh) * | 2019-09-26 | 2019-12-10 | 暨南大学 | 一种诊断脊髓性肌萎缩症的CRISPR-Cas系统及其应用 |
CN110551800A (zh) * | 2018-06-03 | 2019-12-10 | 上海吐露港生物科技有限公司 | 一种耐高温Cas蛋白的用途及靶标核酸分子的检测方法和试剂盒 |
CN110819652A (zh) * | 2019-08-21 | 2020-02-21 | 山东金城生物药业有限公司 | 一种谷氨酸棒杆菌同时基因敲除及基因表达抑制的双功能系统及应用 |
WO2020042901A1 (zh) * | 2018-08-30 | 2020-03-05 | 杭州杰毅生物技术有限公司 | 基于CRISPR-Cas的等温核酸检测方法及试剂盒 |
CN110878343A (zh) * | 2019-12-03 | 2020-03-13 | 国家卫生健康委科学技术研究所 | 一种用于遗传性耳聋致病基因SLC26A4突变快速检测的Cpf1试剂盒及其检测方法 |
CN110894557A (zh) * | 2019-09-20 | 2020-03-20 | 武汉大学 | 基于CRISPR方式检测非洲猪瘟病毒的crRNA及试剂盒 |
CN110894530A (zh) * | 2019-12-25 | 2020-03-20 | 武汉博杰生物医学科技有限公司 | 结肠癌相关分子标志物基因突变的检测试剂盒与检测方法 |
CN111007240A (zh) * | 2019-12-02 | 2020-04-14 | 中国医学科学院北京协和医院 | 一种基于crispr技术的均相酶免疫检验系统及其方法和应用 |
CN111117984A (zh) * | 2018-11-01 | 2020-05-08 | 浙江大学 | 可视化特异检测核酸目标的crispr体系及方法和装置 |
CN111154913A (zh) * | 2018-11-08 | 2020-05-15 | 中山大学 | 用于EB病毒DNA检测的引物和crRNA及其应用 |
CN111257297A (zh) * | 2020-03-31 | 2020-06-09 | 海南大学 | 一种基于Cas14a酶的RNA检测方法 |
CN111304294A (zh) * | 2020-01-17 | 2020-06-19 | 中国医学科学院北京协和医院 | Pcda囊泡结合crispr的快速检测系统及其方法 |
CN111363763A (zh) * | 2020-03-31 | 2020-07-03 | 海南大学 | 一种RNA激活Cas14a酶附带切割效应的方法 |
CN111440793A (zh) * | 2020-03-20 | 2020-07-24 | 武汉博杰生物医学科技有限公司 | 一种新型冠状病毒核酸检测试剂盒 |
CN111455046A (zh) * | 2020-03-13 | 2020-07-28 | 深圳市罗湖区人民医院 | 一种adgrg6增强子突变的核酸检测方法及其试剂盒 |
CN111549177A (zh) * | 2020-04-27 | 2020-08-18 | 广州再生医学与健康广东省实验室 | 用于检测SARS-CoV-2的gRNA及试剂盒 |
CN111560469A (zh) * | 2020-03-30 | 2020-08-21 | 广州和盛医疗科技有限公司 | 一组检测新冠病毒基因的引物及crispr序列组合及其应用 |
CN111615561A (zh) * | 2018-03-15 | 2020-09-01 | 荣研化学株式会社 | 单核苷酸多态性检测用寡核苷酸探针和顺式-反式判别方法 |
CN111662968A (zh) * | 2020-06-23 | 2020-09-15 | 南方科技大学 | 一种基于crispr的无pam序列dna的检测方法及其应用 |
CN111719015A (zh) * | 2020-05-21 | 2020-09-29 | 昆明医科大学第一附属医院 | 一种人类免疫缺陷病毒hiv-1的检测试剂盒 |
CN111855990A (zh) * | 2019-04-29 | 2020-10-30 | 华南师范大学 | 一种基于CRISPR/Cas系统的通用比色核酸检测方法及试剂盒与应用 |
CN111909987A (zh) * | 2020-07-31 | 2020-11-10 | 浙江科途医学科技有限公司 | 用于kif5b-ret融合基因检测的试剂组合物、试剂盒及检测系统 |
CN112251494A (zh) * | 2020-10-09 | 2021-01-22 | 湖南大学 | 基于CRISPR-Cas自催化放大网络的核酸检测方法及其应用 |
CN112301101A (zh) * | 2019-07-24 | 2021-02-02 | 上海吐露港生物科技有限公司 | Crispr多靶标检测方法及其试剂盒 |
CN112301162A (zh) * | 2020-10-22 | 2021-02-02 | 清华-伯克利深圳学院筹备办公室 | 同时检测dna病毒和rna病毒的病毒核酸检测方法 |
CN112639121A (zh) * | 2018-06-26 | 2021-04-09 | 博德研究所 | 基于crispr双切口酶的扩增组合物、系统和方法 |
CN112695073A (zh) * | 2020-12-30 | 2021-04-23 | 上海理工大学 | 一种CRISPR/Cas12a一步核酸检测方法 |
CN112795624A (zh) * | 2020-07-17 | 2021-05-14 | 山东舜丰生物科技有限公司 | 利用含有无碱基间隔物的核酸检测器检测靶核酸的方法 |
CN112831544A (zh) * | 2020-12-31 | 2021-05-25 | 华南农业大学 | 一种基于CRISPR/Cas12a系统的生物检测方法和生物检测装置 |
CN112852980A (zh) * | 2021-04-23 | 2021-05-28 | 上海海关动植物与食品检验检疫技术中心 | 基于crispr荧光法检测大西洋鲑核酸的检测方法及其检测试剂盒 |
CN112899350A (zh) * | 2018-05-14 | 2021-06-04 | 北京艾克伦医疗科技有限公司 | 核酸检测方法和试剂盒 |
CN112986551A (zh) * | 2019-12-11 | 2021-06-18 | 清华大学 | 一种检测混合体系内目标分子浓度的方法及试剂盒 |
CN113046477A (zh) * | 2021-02-26 | 2021-06-29 | 广州医科大学附属第一医院(广州呼吸中心) | 鉴定D614G突变位点的特异性crRNA、体系、试剂盒及应用 |
WO2021154866A1 (en) | 2020-01-27 | 2021-08-05 | Sherlock Biosciences | Improved detection assays |
CN113227367A (zh) * | 2018-08-09 | 2021-08-06 | G+Flas生命科学公司 | 用cas12a蛋白进行基因组工程的组合物和方法 |
WO2021155775A1 (zh) * | 2020-02-03 | 2021-08-12 | 苏州克睿基因生物科技有限公司 | 一种检测靶核酸的方法和试剂盒 |
CN113324956A (zh) * | 2021-04-22 | 2021-08-31 | 上海市肿瘤研究所 | 一种基于crispr技术与框架核酸耦合的模块化检测平台、构建方法及其应用 |
US11174470B2 (en) | 2019-01-04 | 2021-11-16 | Mammoth Biosciences, Inc. | Programmable nuclease improvements and compositions and methods for nucleic acid amplification and detection |
CN113686934A (zh) * | 2021-08-13 | 2021-11-23 | 广东海洋大学 | 一种CRISPR/Cas12a-RCA电化学传感器检测体系及其应用 |
CN113999921A (zh) * | 2021-11-23 | 2022-02-01 | 中国医学科学院输血研究所 | 快速可视化检测福氏志贺氏菌的方法和试剂盒 |
US11273442B1 (en) | 2018-08-01 | 2022-03-15 | Mammoth Biosciences, Inc. | Programmable nuclease compositions and methods of use thereof |
CN114561450A (zh) * | 2020-11-27 | 2022-05-31 | 佳能株式会社 | 检测靶核酸的方法和试剂盒 |
CN114592043A (zh) * | 2022-03-23 | 2022-06-07 | 北京盛因生物科技有限公司 | 一种等温核酸检测酶组合物、试剂盒及其用途和检测方法 |
CN114634972A (zh) * | 2022-05-19 | 2022-06-17 | 舜丰生物科技(海南)有限公司 | 利用Cas酶进行核酸检测的方法 |
CN114807431A (zh) * | 2021-04-15 | 2022-07-29 | 华中农业大学 | 一种改进的基于crispr系统介导的核酸可视化检测技术及其应用 |
US11584955B2 (en) | 2017-07-14 | 2023-02-21 | Shanghai Tolo Biotechnology Company Limited | Application of Cas protein, method for detecting target nucleic acid molecule and kit |
CN115838831A (zh) * | 2022-08-29 | 2023-03-24 | 深圳市海微生物科技有限公司 | FnCas12a突变体在核酸检测中的应用及核酸检测方法 |
CN116297376A (zh) * | 2023-04-25 | 2023-06-23 | 中国科学院过程工程研究所 | 一种光电双模式生物探针及其应用 |
RU2800778C2 (ru) * | 2018-06-03 | 2023-07-28 | Шангхаи Толо Биотехнолоджи Компани Лимитед | Применение высокотермостойкого cas-белка и способ и набор реактивов для обнаружения целевой молекулы нуклеиновой кислоты |
CN116574848A (zh) * | 2023-06-20 | 2023-08-11 | 中国农业科学院兰州兽医研究所 | 一种基于LAMP-CRISPRCas12a检测猪圆环2型病毒的引物及检测方法 |
CN116656784A (zh) * | 2023-07-26 | 2023-08-29 | 南京鸿明生物科技有限公司 | 一种免扩增的腺相关病毒基因组的滴度测量方法 |
Families Citing this family (33)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2020046809A1 (en) * | 2018-08-27 | 2020-03-05 | The Regents Of The University Of California | Reporter nucleic acids for type v crispr-mediated detection |
CN111944879B (zh) * | 2019-05-16 | 2024-05-14 | 华南师范大学 | 一种非疾病诊断目的的基于crispr技术的基因检测方法 |
CN112301102B (zh) * | 2019-07-31 | 2024-06-25 | 华南师范大学 | 一种高特异性的环介导等温扩增方法及其应用 |
CN110982878B (zh) * | 2019-11-29 | 2023-06-13 | 华南师范大学 | CRISPR/Cas13a结合电化学发光系统检测microRNA的方法与应用 |
CN112941153B (zh) * | 2019-12-11 | 2022-09-09 | 清华大学 | 一种dna检测方法 |
CN111197098B (zh) * | 2020-02-20 | 2023-08-22 | 首都医科大学附属北京胸科医院 | 一种从痰液中检测结核分枝杆菌的方法 |
US11851702B2 (en) | 2020-03-23 | 2023-12-26 | The Broad Institute, Inc. | Rapid diagnostics |
EP4165218A1 (en) | 2020-06-12 | 2023-04-19 | Sherlock Biosciences, Inc. | Crispr-based sars-cov-2 detection |
CN111733216B (zh) * | 2020-06-22 | 2023-03-28 | 山东舜丰生物科技有限公司 | 一种提高目标核酸检测效率的方法 |
CN111733287B (zh) * | 2020-06-22 | 2022-10-11 | 天津大学 | 检测发热伴血小板减少综合征病原体核酸的试剂盒 |
CN112795625B (zh) * | 2020-08-28 | 2022-02-08 | 山东舜丰生物科技有限公司 | 基于crispr技术进行多重核酸检测的方法 |
CN116024314A (zh) * | 2020-09-18 | 2023-04-28 | 山东舜丰生物科技有限公司 | 基于crispr技术进行靶核酸多重检测的方法 |
WO2022080343A1 (ja) * | 2020-10-13 | 2022-04-21 | キヤノン株式会社 | 核酸検出装置および核酸の検出方法 |
US20240076712A1 (en) * | 2021-01-08 | 2024-03-07 | Wuhan University | Compositions and methods for instant nucleic acid detection |
EP4291678A1 (en) | 2021-02-09 | 2023-12-20 | Sherlock Biosciences, Inc. | Nucleic acid amplification using promoter primers |
CN113061650B (zh) * | 2021-02-19 | 2024-07-16 | 中国科学院深圳先进技术研究院 | 一种病原体核酸的即时检测系统及方法 |
NO347409B1 (no) * | 2021-04-11 | 2023-10-23 | Glomsroed Mikkel Ante | Fremgangsmåte for påvisning av mikroorganismer eller genfeil |
CN113444817A (zh) * | 2021-05-12 | 2021-09-28 | 中国人民解放军军事科学院军事医学研究院 | 一种基于CRISPR-Cas12a系统的炭疽芽胞杆菌检测方法 |
CN113403424B (zh) * | 2021-05-28 | 2024-01-09 | 华南理工大学 | 基于CRISPR/Cas12a技术快速检测新冠病毒和突变株的方法及试剂盒 |
CN113337489A (zh) * | 2021-06-07 | 2021-09-03 | 广州美格生物科技有限公司 | 一种新型嵌合体TsCas12a蛋白及制备技术 |
CN113373264B (zh) * | 2021-06-10 | 2022-04-12 | 安徽医科大学 | 一种基于CRISPR/Cas系统的新型冠状病毒双靶标快速检测方法及试剂盒 |
CN113355397A (zh) * | 2021-06-24 | 2021-09-07 | 深圳市第二人民医院(深圳市转化医学研究院) | 场效应晶体管芯片、检测核酸的试剂盒和核酸含量的检测方法 |
CN113980957A (zh) * | 2021-07-02 | 2022-01-28 | 苏州系统医学研究所 | 基于CRISPR/Cas12a的单链DNA探针及检测目标核酸的方法 |
CN113388691B (zh) * | 2021-07-23 | 2022-08-30 | 中国疾病预防控制中心传染病预防控制所 | 一种基于PCR扩增和CRISPR-Cas12a的核酸检测方法及应用 |
CN115247206A (zh) * | 2021-10-21 | 2022-10-28 | 河北大学 | 基于连接酶链式反应和基因编辑技术检测核酸标志物的方法 |
WO2023087290A1 (en) * | 2021-11-20 | 2023-05-25 | Agricultural Genomics Institute At Shenzhen, Chinese Academy Of Agricultural Sciences | Methods for nucleic acid detection |
CN114134218B (zh) * | 2021-12-02 | 2022-10-28 | 中国疾病预防控制中心传染病预防控制所 | 一种基于CRISPR-Cas12a的荧光检测方法 |
CN114292843B (zh) * | 2021-12-03 | 2023-07-21 | 中国科学院精密测量科学与技术创新研究院 | 一种基因兴奋剂的CRISPR/Cas12a检测体系及其应用 |
CN114807316B (zh) * | 2022-03-11 | 2023-02-03 | 北京科技大学 | 一种无核酸扩增可视化的rna定量检测方法 |
TW202421795A (zh) | 2022-07-01 | 2024-06-01 | 美商夏洛克生物科學公司 | 環境溫度核酸擴增及偵測 |
CN116240200A (zh) * | 2022-07-01 | 2023-06-09 | 中国科学院基础医学与肿瘤研究所(筹) | 一种基于可编程核酸酶的超灵敏目标核酸富集检测方法 |
CN116179511B (zh) * | 2023-03-10 | 2023-12-22 | 之江实验室 | Cpf1蛋白在制备用于核酸检测的试剂盒中的应用 |
CN117821630A (zh) * | 2024-02-20 | 2024-04-05 | 江苏省家禽科学研究所 | 用于检测肠炎沙门氏菌的核酸检测试剂盒及方法 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105441440A (zh) * | 2012-10-23 | 2016-03-30 | 基因工具股份有限公司 | 包含特异于靶dna的向导rna和cas蛋白质编码核酸或cas蛋白质的用于切割靶dna的组合物及其用途 |
WO2017053762A1 (en) * | 2015-09-24 | 2017-03-30 | Sigma-Aldrich Co. Llc | Methods and reagents for molecular proximity detection using rna-guided nucleic acid binding proteins |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101935697B (zh) * | 2010-04-16 | 2015-11-25 | 中生方政生物技术有限公司 | 用于核酸序列检测的方法和试剂盒 |
CA2893579A1 (en) * | 2012-12-13 | 2014-06-19 | Dow Agrosciences Llc | Dna detection methods for site specific nuclease activity |
EP3250689B1 (en) * | 2015-01-28 | 2020-11-04 | The Regents of The University of California | Methods and compositions for labeling a single-stranded target nucleic acid |
US9790490B2 (en) * | 2015-06-18 | 2017-10-17 | The Broad Institute Inc. | CRISPR enzymes and systems |
CN116814590A (zh) * | 2015-10-22 | 2023-09-29 | 布罗德研究所有限公司 | Vi-b型crispr酶和系统 |
TW201737944A (zh) * | 2015-11-12 | 2017-11-01 | 輝瑞大藥廠 | 使用crispr-cas9之組織特異性基因組工程 |
CN107488710B (zh) * | 2017-07-14 | 2020-09-22 | 上海吐露港生物科技有限公司 | 一种Cas蛋白的用途及靶标核酸分子的检测方法和试剂盒 |
US10253365B1 (en) * | 2017-11-22 | 2019-04-09 | The Regents Of The University Of California | Type V CRISPR/Cas effector proteins for cleaving ssDNAs and detecting target DNAs |
-
2017
- 2017-07-14 CN CN201710573752.0A patent/CN107488710B/zh active Active
- 2017-07-14 CN CN202011092324.4A patent/CN112501254B/zh active Active
-
2018
- 2018-04-12 CA CA3069788A patent/CA3069788A1/en active Pending
- 2018-04-12 SG SG11202000336RA patent/SG11202000336RA/en unknown
- 2018-04-12 CN CN202410308071.1A patent/CN118207297A/zh active Pending
- 2018-04-12 BR BR112020000809-5A patent/BR112020000809A2/pt unknown
- 2018-04-12 EP EP18832456.0A patent/EP3653722A4/en active Pending
- 2018-04-12 US US16/631,157 patent/US20230002811A1/en active Pending
- 2018-04-12 EA EA202090290A patent/EA202090290A1/ru unknown
- 2018-04-12 JP JP2020523474A patent/JP2020530779A/ja active Pending
- 2018-04-12 AU AU2018299445A patent/AU2018299445B2/en active Active
- 2018-04-12 CN CN201880046701.5A patent/CN111094588B/zh active Active
- 2018-04-12 MA MA049579A patent/MA49579A/fr unknown
- 2018-04-12 KR KR1020207003220A patent/KR20200035956A/ko not_active Application Discontinuation
- 2018-04-12 WO PCT/CN2018/082769 patent/WO2019011022A1/zh unknown
- 2018-04-12 MX MX2020000481A patent/MX2020000481A/es unknown
-
2020
- 2020-01-13 IL IL272005A patent/IL272005A/en unknown
- 2020-01-14 PH PH12020500103A patent/PH12020500103A1/en unknown
- 2020-01-31 ZA ZA2020/00682A patent/ZA202000682B/en unknown
-
2022
- 2022-12-20 US US18/085,121 patent/US20230131421A1/en active Pending
-
2023
- 2023-04-13 JP JP2023065743A patent/JP2023080282A/ja active Pending
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105441440A (zh) * | 2012-10-23 | 2016-03-30 | 基因工具股份有限公司 | 包含特异于靶dna的向导rna和cas蛋白质编码核酸或cas蛋白质的用于切割靶dna的组合物及其用途 |
WO2017053762A1 (en) * | 2015-09-24 | 2017-03-30 | Sigma-Aldrich Co. Llc | Methods and reagents for molecular proximity detection using rna-guided nucleic acid binding proteins |
Non-Patent Citations (5)
Title |
---|
ALEXANDRA EAST-SELETSKY 等: "Two Distinct RNase Activities of CRISPR-C2c2 Enable Guide RNA Processing and RNA Detection", 《NATURE》 * |
JONATHAN S. GOOTENBERG 等: "Nucleic acid detection with CRISPR-Cas13a/C2c2", 《SCIENCE》 * |
PU GAO 等: "Type V CRISPR-Cas Cpf1 endonuclease employs a unique mechanism for crRNA-mediated target DNA recognition", 《CELL RESEARCH》 * |
SHI-YUAN LI 等: "CRISPR-Cas12a-assisted nucleic acid detection", 《CELL DISCOVERY》 * |
李诗渊 等: "合成生物学技术的研究进展——DNA 合成、组装与基因组编辑", 《生物工程学报》 * |
Cited By (109)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2019011022A1 (zh) * | 2017-07-14 | 2019-01-17 | 上海吐露港生物科技有限公司 | 一种Cas蛋白的用途及靶标核酸分子的检测方法和试剂盒 |
US11584955B2 (en) | 2017-07-14 | 2023-02-21 | Shanghai Tolo Biotechnology Company Limited | Application of Cas protein, method for detecting target nucleic acid molecule and kit |
US11447824B2 (en) | 2017-11-22 | 2022-09-20 | The Regents Of The University Of California | Type V CRISPR/Cas effector proteins for cleaving ssDNAs and detecting target DNAs |
WO2019104058A1 (en) | 2017-11-22 | 2019-05-31 | The Regents Of The University Of California | Type v crispr/cas effector proteins for cleaving ssdnas and detecting target dnas |
US10253365B1 (en) | 2017-11-22 | 2019-04-09 | The Regents Of The University Of California | Type V CRISPR/Cas effector proteins for cleaving ssDNAs and detecting target DNAs |
US11118224B2 (en) | 2017-11-22 | 2021-09-14 | The Regents Of The University Of California | Type V CRISPR/Cas effector proteins for cleaving ssDNAs and detecting target DNAs |
CN111630163A (zh) * | 2017-11-22 | 2020-09-04 | 加利福尼亚大学董事会 | 用于切割ssdna以及检测靶dna的v型crispr/cas效应蛋白 |
CN108220400A (zh) * | 2018-01-02 | 2018-06-29 | 武汉启丰生物技术有限公司 | 一种基于CRISPR-Cas10核酸蛋白复合物的快捷核酸检测定量法 |
CN111615561A (zh) * | 2018-03-15 | 2020-09-01 | 荣研化学株式会社 | 单核苷酸多态性检测用寡核苷酸探针和顺式-反式判别方法 |
CN111615561B (zh) * | 2018-03-15 | 2024-06-04 | 荣研化学株式会社 | 单核苷酸多态性检测用寡核苷酸探针和顺式-反式判别方法 |
US11851701B2 (en) | 2018-03-15 | 2023-12-26 | Eiken Kagaku Kabushiki Kaisha | Oligonucleotide probe for detecting single nucleotide polymorphisms, and method for determining cis-trans configuration |
CN108796036A (zh) * | 2018-04-03 | 2018-11-13 | 上海交通大学 | 基于原核Argonaute蛋白的核酸检测方法及其应用 |
CN108588182A (zh) * | 2018-04-13 | 2018-09-28 | 中国科学院深圳先进技术研究院 | 基于crispr-链取代的等温扩增及检测技术 |
CN112941155B (zh) * | 2018-05-14 | 2023-07-14 | 北京艾克伦医疗科技有限公司 | 具有茎环结构的dna引物对及其应用 |
CN112899350B (zh) * | 2018-05-14 | 2023-01-24 | 北京艾克伦医疗科技有限公司 | 核酸检测方法和试剂盒 |
CN112941155A (zh) * | 2018-05-14 | 2021-06-11 | 北京艾克伦医疗科技有限公司 | 具有茎环结构的dna引物对及其应用 |
CN112899350A (zh) * | 2018-05-14 | 2021-06-04 | 北京艾克伦医疗科技有限公司 | 核酸检测方法和试剂盒 |
US12104204B2 (en) | 2018-06-03 | 2024-10-01 | Shanghai Tolo Biotechnology Company Limited | Use of high-temperature-resistant Cas protein, and method and reagent kit for detecting target nucleic acid molecule |
RU2800778C2 (ru) * | 2018-06-03 | 2023-07-28 | Шангхаи Толо Биотехнолоджи Компани Лимитед | Применение высокотермостойкого cas-белка и способ и набор реактивов для обнаружения целевой молекулы нуклеиновой кислоты |
EP3805403A4 (en) * | 2018-06-03 | 2022-05-04 | Shanghai Tolo Biotechnology Company Limited | USE OF A HIGH TEMPERATURE RESISTANT CAS PROTEIN, AND METHOD AND REAGENT KIT FOR DETECTING A TARGET NUCLEIC ACID MOLECULE |
JP7431453B2 (ja) | 2018-06-03 | 2024-02-15 | シャンハイ トロ バイオテクノロジー カンパニー リミテッド | 耐高温Casタンパク質の使用および標的核酸分子の検出方法とキット |
CN110551800A (zh) * | 2018-06-03 | 2019-12-10 | 上海吐露港生物科技有限公司 | 一种耐高温Cas蛋白的用途及靶标核酸分子的检测方法和试剂盒 |
WO2019233385A1 (zh) * | 2018-06-03 | 2019-12-12 | 上海吐露港生物科技有限公司 | 一种耐高温Cas蛋白的用途及靶标核酸分子的检测方法和试剂盒 |
AU2019280305B2 (en) * | 2018-06-03 | 2023-07-20 | Shanghai Tolo Biotechnology Company Limited | Use of high-temperature-resistant Cas protein, and method and reagent kit for detecting target nucleic acid molecule |
JP2021528098A (ja) * | 2018-06-03 | 2021-10-21 | シャンハイ トロ バイオテクノロジー カンパニー リミテッドShanghai Tolo Biotechnology Company Limited | 耐高温Casタンパク質の使用および標的核酸分子の検出方法とキット |
CN112236530A (zh) * | 2018-06-03 | 2021-01-15 | 上海吐露港生物科技有限公司 | 一种耐高温Cas蛋白的用途及靶标核酸分子的检测方法和试剂盒 |
CN112639121A (zh) * | 2018-06-26 | 2021-04-09 | 博德研究所 | 基于crispr双切口酶的扩增组合物、系统和方法 |
US11273442B1 (en) | 2018-08-01 | 2022-03-15 | Mammoth Biosciences, Inc. | Programmable nuclease compositions and methods of use thereof |
US11761029B2 (en) | 2018-08-01 | 2023-09-19 | Mammoth Biosciences, Inc. | Programmable nuclease compositions and methods of use thereof |
CN113227367A (zh) * | 2018-08-09 | 2021-08-06 | G+Flas生命科学公司 | 用cas12a蛋白进行基因组工程的组合物和方法 |
CN113227367B (zh) * | 2018-08-09 | 2023-05-12 | G+Flas生命科学公司 | 用cas12a蛋白进行基因组工程的组合物和方法 |
WO2020042901A1 (zh) * | 2018-08-30 | 2020-03-05 | 杭州杰毅生物技术有限公司 | 基于CRISPR-Cas的等温核酸检测方法及试剂盒 |
WO2020056924A1 (zh) * | 2018-09-20 | 2020-03-26 | 中国科学院动物研究所 | 核酸检测方法 |
JP7500549B2 (ja) | 2018-09-20 | 2024-06-17 | インスティテュート オブ ズーオロジー、チャイニーズ アカデミー オブ サイエンシーズ | 核酸を検出する方法 |
CN109837328A (zh) * | 2018-09-20 | 2019-06-04 | 中国科学院动物研究所 | 核酸检测方法 |
CN109837328B (zh) * | 2018-09-20 | 2021-07-27 | 中国科学院动物研究所 | 核酸检测方法 |
JP2022501039A (ja) * | 2018-09-20 | 2022-01-06 | インスティテュート オブ ズーオロジー、チャイニーズ アカデミー オブ サイエンシーズ | 核酸を検出する方法 |
CN111117984A (zh) * | 2018-11-01 | 2020-05-08 | 浙江大学 | 可视化特异检测核酸目标的crispr体系及方法和装置 |
CN111154913B (zh) * | 2018-11-08 | 2023-04-18 | 中山大学 | 用于EB病毒DNA检测的引物和crRNA及其应用 |
CN111154913A (zh) * | 2018-11-08 | 2020-05-15 | 中山大学 | 用于EB病毒DNA检测的引物和crRNA及其应用 |
CN109251963A (zh) * | 2018-11-12 | 2019-01-22 | 复旦大学 | 恒温检测细胞培养液中支原体污染的方法及试剂盒 |
CN109251963B (zh) * | 2018-11-12 | 2022-11-18 | 复旦大学 | 恒温检测细胞培养液中支原体污染的方法及试剂盒 |
CN109666662A (zh) * | 2018-12-12 | 2019-04-23 | 广州普世利华科技有限公司 | 新型ScCas12a在核酸检测方面的应用 |
US11174470B2 (en) | 2019-01-04 | 2021-11-16 | Mammoth Biosciences, Inc. | Programmable nuclease improvements and compositions and methods for nucleic acid amplification and detection |
CN109628564B (zh) * | 2019-02-25 | 2022-02-01 | 北京市理化分析测试中心 | 一种用于检测snp多态性的引物组和利用引物组检测snp多态性的方法 |
CN109628564A (zh) * | 2019-02-25 | 2019-04-16 | 北京市理化分析测试中心 | 一种用于检测snp多态性的引物组和利用引物组检测snp多态性的方法 |
CN109929949A (zh) * | 2019-03-14 | 2019-06-25 | 广州医科大学附属肿瘤医院 | 基于CRISPR-Cas12a和G四链体-血红素的EBV的检测方法及应用 |
CN110117818A (zh) * | 2019-04-16 | 2019-08-13 | 天津大学 | 检测单基因突变的crispr高通量生物芯片 |
CN110029194A (zh) * | 2019-04-24 | 2019-07-19 | 安邦(厦门)生物科技有限公司 | 基于CRISPR-Cas基因编辑技术的连续荧光监测检测方法及装置 |
CN111855990B (zh) * | 2019-04-29 | 2023-06-27 | 华南师范大学 | 一种基于CRISPR/Cas系统的通用比色核酸检测方法及试剂盒与应用 |
CN111855990A (zh) * | 2019-04-29 | 2020-10-30 | 华南师范大学 | 一种基于CRISPR/Cas系统的通用比色核酸检测方法及试剂盒与应用 |
CN110257556A (zh) * | 2019-04-30 | 2019-09-20 | 广州普世利华科技有限公司 | 一种性传播疾病致病病原的核酸检测试剂盒 |
CN110257556B (zh) * | 2019-04-30 | 2023-04-18 | 广州普世利华科技有限公司 | 一种性传播疾病致病病原的核酸检测试剂盒 |
CN110195102A (zh) * | 2019-04-30 | 2019-09-03 | 广州普世利华科技有限公司 | 一种β地中海贫血基因分型方法 |
CN110157821A (zh) * | 2019-05-06 | 2019-08-23 | 浙江大学 | 一种用于人葡萄球菌检测的试剂盒 |
CN110157821B (zh) * | 2019-05-06 | 2020-08-28 | 浙江大学 | 一种用于人葡萄球菌检测的试剂盒 |
CN110317890A (zh) * | 2019-06-24 | 2019-10-11 | 浙江大学 | 一种用于粪肠球菌检测的试剂盒 |
CN110241238A (zh) * | 2019-06-24 | 2019-09-17 | 浙江大学 | 一种用于化脓链球菌检测的试剂盒 |
CN110358848A (zh) * | 2019-06-24 | 2019-10-22 | 浙江大学 | 一种用于屎肠球菌检测的试剂盒 |
CN110331219A (zh) * | 2019-06-24 | 2019-10-15 | 浙江大学 | 一种用于铜绿假单胞菌检测的试剂盒 |
CN114174535A (zh) * | 2019-07-24 | 2022-03-11 | 上海吐露港生物科技有限公司 | Crispr多靶标检测方法及其试剂盒 |
CN112301101A (zh) * | 2019-07-24 | 2021-02-02 | 上海吐露港生物科技有限公司 | Crispr多靶标检测方法及其试剂盒 |
CN112301101B (zh) * | 2019-07-24 | 2024-07-19 | 上海吐露港生物科技有限公司 | Crispr多靶标检测方法及其试剂盒 |
CN110819652A (zh) * | 2019-08-21 | 2020-02-21 | 山东金城生物药业有限公司 | 一种谷氨酸棒杆菌同时基因敲除及基因表达抑制的双功能系统及应用 |
CN110894557A (zh) * | 2019-09-20 | 2020-03-20 | 武汉大学 | 基于CRISPR方式检测非洲猪瘟病毒的crRNA及试剂盒 |
CN110551812B (zh) * | 2019-09-26 | 2020-08-18 | 暨南大学 | 一种诊断脊髓性肌萎缩症的CRISPR-Cas系统及其应用 |
CN110551812A (zh) * | 2019-09-26 | 2019-12-10 | 暨南大学 | 一种诊断脊髓性肌萎缩症的CRISPR-Cas系统及其应用 |
CN111007240A (zh) * | 2019-12-02 | 2020-04-14 | 中国医学科学院北京协和医院 | 一种基于crispr技术的均相酶免疫检验系统及其方法和应用 |
CN110878343B (zh) * | 2019-12-03 | 2023-04-07 | 国家卫生健康委科学技术研究所 | 一种用于遗传性耳聋致病基因SLC26A4突变快速检测的Cpf1试剂盒及其检测方法 |
CN110878343A (zh) * | 2019-12-03 | 2020-03-13 | 国家卫生健康委科学技术研究所 | 一种用于遗传性耳聋致病基因SLC26A4突变快速检测的Cpf1试剂盒及其检测方法 |
CN112986551B (zh) * | 2019-12-11 | 2022-09-27 | 北京聚树生物科技有限公司 | 一种检测混合体系内目标分子浓度的方法及试剂盒 |
CN112986551A (zh) * | 2019-12-11 | 2021-06-18 | 清华大学 | 一种检测混合体系内目标分子浓度的方法及试剂盒 |
CN110894530A (zh) * | 2019-12-25 | 2020-03-20 | 武汉博杰生物医学科技有限公司 | 结肠癌相关分子标志物基因突变的检测试剂盒与检测方法 |
CN111304294A (zh) * | 2020-01-17 | 2020-06-19 | 中国医学科学院北京协和医院 | Pcda囊泡结合crispr的快速检测系统及其方法 |
WO2021154866A1 (en) | 2020-01-27 | 2021-08-05 | Sherlock Biosciences | Improved detection assays |
WO2021155775A1 (zh) * | 2020-02-03 | 2021-08-12 | 苏州克睿基因生物科技有限公司 | 一种检测靶核酸的方法和试剂盒 |
CN111455046A (zh) * | 2020-03-13 | 2020-07-28 | 深圳市罗湖区人民医院 | 一种adgrg6增强子突变的核酸检测方法及其试剂盒 |
CN111440793A (zh) * | 2020-03-20 | 2020-07-24 | 武汉博杰生物医学科技有限公司 | 一种新型冠状病毒核酸检测试剂盒 |
CN111560469A (zh) * | 2020-03-30 | 2020-08-21 | 广州和盛医疗科技有限公司 | 一组检测新冠病毒基因的引物及crispr序列组合及其应用 |
CN111363763A (zh) * | 2020-03-31 | 2020-07-03 | 海南大学 | 一种RNA激活Cas14a酶附带切割效应的方法 |
CN111257297A (zh) * | 2020-03-31 | 2020-06-09 | 海南大学 | 一种基于Cas14a酶的RNA检测方法 |
CN111549177A (zh) * | 2020-04-27 | 2020-08-18 | 广州再生医学与健康广东省实验室 | 用于检测SARS-CoV-2的gRNA及试剂盒 |
CN111719015A (zh) * | 2020-05-21 | 2020-09-29 | 昆明医科大学第一附属医院 | 一种人类免疫缺陷病毒hiv-1的检测试剂盒 |
CN111662968A (zh) * | 2020-06-23 | 2020-09-15 | 南方科技大学 | 一种基于crispr的无pam序列dna的检测方法及其应用 |
CN112795624A (zh) * | 2020-07-17 | 2021-05-14 | 山东舜丰生物科技有限公司 | 利用含有无碱基间隔物的核酸检测器检测靶核酸的方法 |
CN111909987A (zh) * | 2020-07-31 | 2020-11-10 | 浙江科途医学科技有限公司 | 用于kif5b-ret融合基因检测的试剂组合物、试剂盒及检测系统 |
CN112251494B (zh) * | 2020-10-09 | 2022-09-02 | 湖南大学 | 基于CRISPR-Cas自催化放大网络的核酸检测方法及其应用 |
CN112251494A (zh) * | 2020-10-09 | 2021-01-22 | 湖南大学 | 基于CRISPR-Cas自催化放大网络的核酸检测方法及其应用 |
CN112301162A (zh) * | 2020-10-22 | 2021-02-02 | 清华-伯克利深圳学院筹备办公室 | 同时检测dna病毒和rna病毒的病毒核酸检测方法 |
CN112301162B (zh) * | 2020-10-22 | 2023-09-22 | 清华-伯克利深圳学院筹备办公室 | 同时检测dna病毒和rna病毒的病毒核酸检测方法 |
CN114561450A (zh) * | 2020-11-27 | 2022-05-31 | 佳能株式会社 | 检测靶核酸的方法和试剂盒 |
CN112695073A (zh) * | 2020-12-30 | 2021-04-23 | 上海理工大学 | 一种CRISPR/Cas12a一步核酸检测方法 |
CN112831544A (zh) * | 2020-12-31 | 2021-05-25 | 华南农业大学 | 一种基于CRISPR/Cas12a系统的生物检测方法和生物检测装置 |
CN113046477A (zh) * | 2021-02-26 | 2021-06-29 | 广州医科大学附属第一医院(广州呼吸中心) | 鉴定D614G突变位点的特异性crRNA、体系、试剂盒及应用 |
CN114807431A (zh) * | 2021-04-15 | 2022-07-29 | 华中农业大学 | 一种改进的基于crispr系统介导的核酸可视化检测技术及其应用 |
CN113324956A (zh) * | 2021-04-22 | 2021-08-31 | 上海市肿瘤研究所 | 一种基于crispr技术与框架核酸耦合的模块化检测平台、构建方法及其应用 |
CN112852980A (zh) * | 2021-04-23 | 2021-05-28 | 上海海关动植物与食品检验检疫技术中心 | 基于crispr荧光法检测大西洋鲑核酸的检测方法及其检测试剂盒 |
CN112852980B (zh) * | 2021-04-23 | 2021-11-26 | 上海海关动植物与食品检验检疫技术中心 | 基于crispr荧光法检测大西洋鲑核酸的检测方法及其检测试剂盒 |
CN113686934A (zh) * | 2021-08-13 | 2021-11-23 | 广东海洋大学 | 一种CRISPR/Cas12a-RCA电化学传感器检测体系及其应用 |
CN113999921A (zh) * | 2021-11-23 | 2022-02-01 | 中国医学科学院输血研究所 | 快速可视化检测福氏志贺氏菌的方法和试剂盒 |
CN113999921B (zh) * | 2021-11-23 | 2023-06-27 | 中国医学科学院输血研究所 | 快速可视化检测福氏志贺氏菌的方法和试剂盒 |
CN114592043A (zh) * | 2022-03-23 | 2022-06-07 | 北京盛因生物科技有限公司 | 一种等温核酸检测酶组合物、试剂盒及其用途和检测方法 |
CN114634972A (zh) * | 2022-05-19 | 2022-06-17 | 舜丰生物科技(海南)有限公司 | 利用Cas酶进行核酸检测的方法 |
CN115838831A (zh) * | 2022-08-29 | 2023-03-24 | 深圳市海微生物科技有限公司 | FnCas12a突变体在核酸检测中的应用及核酸检测方法 |
CN116297376B (zh) * | 2023-04-25 | 2023-08-29 | 中国科学院过程工程研究所 | 一种光电双模式生物探针及其应用 |
CN116297376A (zh) * | 2023-04-25 | 2023-06-23 | 中国科学院过程工程研究所 | 一种光电双模式生物探针及其应用 |
CN116574848A (zh) * | 2023-06-20 | 2023-08-11 | 中国农业科学院兰州兽医研究所 | 一种基于LAMP-CRISPRCas12a检测猪圆环2型病毒的引物及检测方法 |
CN116656784B (zh) * | 2023-07-26 | 2023-10-20 | 南京鸿明生物科技有限公司 | 一种免扩增的腺相关病毒基因组的滴度测量方法 |
CN116656784A (zh) * | 2023-07-26 | 2023-08-29 | 南京鸿明生物科技有限公司 | 一种免扩增的腺相关病毒基因组的滴度测量方法 |
Also Published As
Publication number | Publication date |
---|---|
JP2020530779A (ja) | 2020-10-29 |
EP3653722A4 (en) | 2021-04-21 |
CN112501254A (zh) | 2021-03-16 |
EA202090290A1 (ru) | 2020-07-14 |
CN107488710B (zh) | 2020-09-22 |
CN111094588A (zh) | 2020-05-01 |
AU2018299445B2 (en) | 2024-05-30 |
CN112501254B (zh) | 2024-07-19 |
MX2020000481A (es) | 2020-09-10 |
US20230131421A1 (en) | 2023-04-27 |
CA3069788A1 (en) | 2019-01-17 |
SG11202000336RA (en) | 2020-02-27 |
BR112020000809A2 (pt) | 2020-09-08 |
US20230002811A1 (en) | 2023-01-05 |
CN118207297A (zh) | 2024-06-18 |
ZA202000682B (en) | 2021-01-27 |
CN111094588B (zh) | 2024-04-05 |
KR20200035956A (ko) | 2020-04-06 |
NZ760987A (en) | 2024-03-22 |
EP3653722A1 (en) | 2020-05-20 |
MA49579A (fr) | 2020-05-20 |
JP2023080282A (ja) | 2023-06-08 |
PH12020500103A1 (en) | 2020-10-19 |
IL272005A (en) | 2020-02-27 |
AU2018299445A1 (en) | 2020-02-06 |
WO2019011022A1 (zh) | 2019-01-17 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN107488710A (zh) | 一种Cas蛋白的用途及靶标核酸分子的检测方法和试剂盒 | |
CN110551800B (zh) | 一种耐高温Cas蛋白的用途及靶标核酸分子的检测方法和试剂盒 | |
Gill et al. | Nucleic acid isothermal amplification technologies—a review | |
US10072260B2 (en) | Target enrichment of randomly sheared genomic DNA fragments | |
CN101638685B (zh) | 交叉引物扩增靶核酸序列的方法及用于扩增靶核酸序列的试剂盒及其应用 | |
US8906621B2 (en) | Cross priming amplification of target nucleic acids | |
US11584955B2 (en) | Application of Cas protein, method for detecting target nucleic acid molecule and kit | |
EP2722401B1 (en) | Addition of an adaptor by invasive cleavage | |
JP7539770B2 (ja) | ゲノム再編成検出のための配列決定方法 | |
EP3476938B1 (en) | Method and kit for synthesizing nucleic acid under constant temperature conditions | |
CN114250276B (zh) | 基于指数扩增反应和Argonaute核酸酶的microRNA检测体系及方法 | |
CN111278974B (zh) | 钩状探针、核酸连接方法以及测序文库的构建方法 | |
CN110564823B (zh) | 一种dna恒温扩增方法及试剂盒 | |
CN114085892B (zh) | 用于检测靶标核酸分子的可视化检测体系、试剂或试剂盒及检测方法 | |
CN112391449B (zh) | 一种引物设计灵活的多功能dna恒温扩增方法及其应用 | |
US9212398B2 (en) | Cross priming amplification of target nucleic acids | |
CN116254327A (zh) | 一种Argonaute介导链置换指数扩增方法及应用 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
CB03 | Change of inventor or designer information | ||
CB03 | Change of inventor or designer information |
Inventor after: Cheng Qiuxiang Inventor after: Li Shiyuan Inventor after: Wang Jin Inventor after: Li Xiaoyan Inventor before: Cheng Qiuxiang |
|
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
TA01 | Transfer of patent application right | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20180124 Address after: 201899 Shanghai city Jiading District Juyuan new tree screen 588 Lane Road No. 3012 room 51 Applicant after: SHANGHAI TOLO BIOTECHNOLOGY Co.,Ltd. Applicant after: SHANGHAI INSTITUTES FOR BIOLOGICAL SCIENCES, CHINESE ACADEMY OF SCIENCES Address before: 201899 Shanghai city Jiading District Juyuan new tree screen 588 Lane Road No. 3012 room 51 Applicant before: SHANGHAI TOLO BIOTECHNOLOGY Co.,Ltd. |
|
CB03 | Change of inventor or designer information |
Inventor after: Wang Jin Inventor after: Cheng Qiuxiang Inventor after: Li Shiyuan Inventor after: Li Xiaoyan Inventor before: Cheng Qiuxiang Inventor before: Li Shiyuan Inventor before: Wang Jin Inventor before: Li Xiaoyan |
|
CB03 | Change of inventor or designer information | ||
TA01 | Transfer of patent application right | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20190422 Address after: Room 3012, 51Lane 588, Shuping Road, Juyuan New District, Jiading District, Shanghai, 201899 Applicant after: SHANGHAI TOLO BIOTECHNOLOGY Co.,Ltd. Address before: Room 3012, 51Lane 588, Shuping Road, Juyuan New District, Jiading District, Shanghai, 201899 Applicant before: SHANGHAI TOLO BIOTECHNOLOGY Co.,Ltd. Applicant before: SHANGHAI INSTITUTES FOR BIOLOGICAL SCIENCES, CHINESE ACADEMY OF SCIENCES |
|
GR01 | Patent grant | ||
GR01 | Patent grant | ||
EE01 | Entry into force of recordation of patent licensing contract | ||
EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20171219 Assignee: Shanghai Berger Medical Technology Co.,Ltd. Assignor: SHANGHAI TOLO BIOTECHNOLOGY Co.,Ltd. Contract record no.: X2023990000070 Denomination of invention: Use of Cas protein and detection method and kit of target nucleic acid molecule Granted publication date: 20200922 License type: Common License Record date: 20230112 |
|
EE01 | Entry into force of recordation of patent licensing contract | ||
EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20171219 Assignee: Beijing yuanjingtaike Biotechnology Co.,Ltd. Assignor: SHANGHAI TOLO BIOTECHNOLOGY Co.,Ltd. Contract record no.: X2023980044164 Denomination of invention: Application of a Cas protein and detection method and kit for target nucleic acid molecules Granted publication date: 20200922 License type: Common License Record date: 20231023 |