CN104975089A - 人外周血微泡中的mirna表达和其用途 - Google Patents
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| CN109295213A (zh) * | 2018-11-01 | 2019-02-01 | 中国人民解放军第00医院 | 免疫性血小板减少症的miRNA标志物及试剂盒和应用 |
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| EP2371971B1 (en) | 2006-03-20 | 2013-11-27 | The Ohio State University Research Foundation | Microrna fingerprints during human megakaryocytopoiesis |
| IL282783B2 (en) | 2006-05-18 | 2023-09-01 | Caris Mpi Inc | A system and method for determining a personalized medical intervention for a disease stage |
| US8768629B2 (en) | 2009-02-11 | 2014-07-01 | Caris Mpi, Inc. | Molecular profiling of tumors |
| AU2008207735B2 (en) | 2007-01-26 | 2013-10-03 | University Of Louisville Research Foundation, Inc. | Modification of exosomal components for use as a vaccine |
| AU2008266014B2 (en) * | 2007-06-15 | 2013-06-06 | The Ohio State University Research Foundation | Oncogenic ALL-1 fusion proteins for targeting drosha-mediated microRNA processing |
| WO2009015357A1 (en) | 2007-07-25 | 2009-01-29 | University Of Louisville Research Foundation, Inc. | Exosome-associated microrna as a diagnostic marker |
| EP2173908B1 (en) | 2007-08-03 | 2016-01-06 | The Ohio State University Research Foundation | Ultraconserved regions encoding ncrnas |
| US20100255514A1 (en) | 2007-08-16 | 2010-10-07 | The Royal Institution For The Advancement Of Learning/Mcgill University | Tumor cell-derived microvesicles |
| US9186405B2 (en) | 2007-08-16 | 2015-11-17 | The Royal Institution For The Advancement Of Learning/Mcgill University | Tumor cell-derived microvesicles |
| BRPI0816852A2 (pt) | 2007-09-14 | 2017-06-06 | Univ Ohio State Res Found | método de diagnosticar ou prognosticar doença ou desordem em sujeito, biomarcador, método para determinar e/ou prever se sujeito tem diferenciação da desordem, biomarcador para desordem pulmonar, método para diagnosticar se sujeito tem, ou está em risco de desenvolver doença ou desordem, método de inibir a proliferação de doença ou desordem e método para identificar agente terapêutico do câncer. |
| CN101424640B (zh) * | 2007-11-02 | 2012-07-25 | 江苏命码生物科技有限公司 | 血清中微小核糖核酸的检测方法和用于检测的试剂盒、生物芯片及其制作和应用方法 |
| ES2936256T3 (es) * | 2008-02-01 | 2023-03-15 | Massachusetts Gen Hospital | Uso de microvesículas en el diagnóstico, y pronóstico de enfermedades y afecciones médicas |
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| EP2358912B1 (en) | 2008-10-30 | 2016-10-12 | Caris Life Sciences Switzerland Holdings GmbH | Methods for assessing rna patterns |
| US20130178383A1 (en) * | 2008-11-12 | 2013-07-11 | David Spetzler | Vesicle isolation methods |
| CN102301002A (zh) | 2008-11-12 | 2011-12-28 | 卡里斯生命科学卢森堡控股有限责任公司 | 使用外来体来确定表现型的方法和系统 |
| RU2400203C1 (ru) * | 2009-04-17 | 2010-09-27 | Илья Николаевич Медведев | Способ уменьшения содержания микровезикул в крови при артериальной гипертонии, дислипидемии и абдоминальном ожирении |
| RU2400209C1 (ru) * | 2009-04-17 | 2010-09-27 | Илья Николаевич Медведев | Способ коррекции уровня микровезикул в крови при артериальной гипертонии, дислипидемии и сахарном диабете ii типа |
| CN104087662A (zh) * | 2009-04-29 | 2014-10-08 | 阿姆斯特丹大学学术医学中心 | 对抗、预防和/或测定心力衰竭或心力衰竭的风险的工具和方法 |
| EP2336353A1 (en) * | 2009-12-17 | 2011-06-22 | febit holding GmbH | miRNA fingerprints in the diagnosis of diseases |
| SG177677A1 (en) * | 2009-07-16 | 2012-02-28 | Gen Hospital Corp | Nucleic acid analysis |
| WO2011031877A1 (en) | 2009-09-09 | 2011-03-17 | The General Hospital Corporation | Use of microvesicles in analyzing nucleic acid profiles |
| WO2011031892A1 (en) | 2009-09-09 | 2011-03-17 | The General Hospital Corporation | Use of microvesicles in analyzing kras mutations |
| JP6143041B2 (ja) * | 2009-09-30 | 2017-06-07 | 公益財団法人ヒューマンサイエンス振興財団 | 大腸がん検査キット |
| WO2011039757A2 (en) * | 2009-10-04 | 2011-04-07 | Rosetta Genomics Ltd. | Compositions and methods for prognosis of renal cancer |
| EP2496714B1 (en) * | 2009-11-04 | 2016-08-31 | DiamiR, LLC | Methods of using micro-rna from bodily fluids for diagnosis and monitoring of mild cognitive impairment |
| AU2010321555B2 (en) | 2009-11-23 | 2015-10-15 | The Ohio State University | Materials and methods useful for affecting tumor cell growth, migration and invasion |
| EP2327783A1 (en) * | 2009-11-27 | 2011-06-01 | Universitätsklinikum Freiburg | Pharmaceutical composition comprising miRNA-100 and its use in the modulation of blood vessel growth |
| CN102080086B (zh) * | 2009-12-01 | 2012-12-26 | 中国科学院上海药物研究所 | 人miR-133a反义核酸及其应用 |
| CN102080083B (zh) * | 2009-12-01 | 2013-02-27 | 中国科学院上海药物研究所 | 人miR-149反义核酸及其应用 |
| US20120302626A1 (en) * | 2009-12-04 | 2012-11-29 | Sandeep Dave | Microrna and use thereof in identification of b cell malignancies |
| CN102892898B (zh) * | 2009-12-24 | 2016-03-09 | 复旦大学 | 用于肝细胞癌诊断的含微rna生物标记的诊断试剂盒和方法 |
| WO2011076141A1 (en) * | 2009-12-24 | 2011-06-30 | Fudan University | Diagnostic kits comprising microrna biomarkers and methods for diagnosis of hepatocellular cancer |
| WO2011076147A1 (en) * | 2009-12-24 | 2011-06-30 | Fudan University | Plasma-based micro-rna biomarkers and methods for early detection of colorectal cancer |
| CN102892897B (zh) * | 2009-12-24 | 2016-08-03 | 复旦大学 | 用于肺癌的微rna表达谱分析的组合物和方法 |
| CN102770560B (zh) * | 2009-12-24 | 2015-11-25 | 复旦大学 | 用于早期检测结直肠癌的基于血浆的微rna生物标记及方法 |
| EP2341145A1 (en) * | 2009-12-30 | 2011-07-06 | febit holding GmbH | miRNA fingerprint in the diagnosis of diseases |
| EP2354246A1 (en) | 2010-02-05 | 2011-08-10 | febit holding GmbH | miRNA in the diagnosis of ovarian cancer |
| CA2791905A1 (en) | 2010-03-01 | 2011-09-09 | Caris Life Sciences Luxembourg Holdings, S.A.R.L. | Biomarkers for theranostics |
| EP2363136A1 (en) * | 2010-03-02 | 2011-09-07 | Fresenius Medical Care Deutschland GmbH | Microvesicles (MVs) derived from adult stem cells for use in the therapeutic treatment of a tumor disease |
| JP2011211955A (ja) * | 2010-03-31 | 2011-10-27 | Toray Ind Inc | 肝ガン患者予後予測用組成物及び方法 |
| CA2795776A1 (en) * | 2010-04-06 | 2011-10-13 | Caris Life Sciences Luxembourg Holdings, S.A.R.L. | Circulating biomarkers for disease |
| EP2559442B1 (en) * | 2010-04-13 | 2017-12-06 | Jiangsu Mingma Biotech Co., Ltd | Method for regulating microrna content in organisms and uses thereof |
| US20130108646A1 (en) * | 2010-05-04 | 2013-05-02 | Medimmune, Llc | Optimized degenerative muscle disease diagnostics and treatments |
| CA2801342A1 (en) | 2010-06-04 | 2011-12-08 | Eric N. Olson | Regulation of metabolism by mir-378 |
| US20110318742A1 (en) * | 2010-06-23 | 2011-12-29 | The Chinese University Of Hong Kong | Micro rna markers for colorectal cancer |
| CA2802738A1 (en) * | 2010-06-24 | 2011-12-29 | The Ohio State University | Chronic lymphocytic leukemia modeled in mouse by targeted mir-29 expression |
| WO2012031008A2 (en) | 2010-08-31 | 2012-03-08 | The General Hospital Corporation | Cancer-related biological materials in microvesicles |
| US20130302404A1 (en) * | 2010-09-27 | 2013-11-14 | Devrim Gozuacik | Use of mirnas for the diagnosis, prophylaxis, treatment and follow-up of diseases involving macroautophagy abnormalities |
| EP2638057B1 (en) | 2010-11-10 | 2019-03-06 | Exosome Diagnostics, Inc. | Method for isolation of nucleic acid containing particles and extraction of nucleic acids therefrom |
| EP2638912A4 (en) * | 2010-11-12 | 2015-01-21 | Nat Univ Corp Ehime Univ | COMPOSITIONS WITH AN ANTISENSE OLIGONUCLEOTIDE AGAINST MICRO RNA |
| CN103648505B (zh) | 2010-11-12 | 2016-09-28 | 俄亥俄州立大学研究基金会 | 与微rna-21、错配修复和结肠直肠癌相关的材料和方法 |
| AU2011329066B2 (en) | 2010-11-15 | 2017-03-09 | The Ohio State University Research Foundation | Controlled release mucoadhesive systems |
| AR084319A1 (es) | 2010-12-15 | 2013-05-08 | Miragen Therapeutics | INHIBIDORES DE microARN (miARN O miR) QUE COMPRENDEN NUCLEOTIDOS BLOQUEADOS |
| CN102140465B (zh) * | 2010-12-30 | 2013-06-12 | 苏州吉玛基因股份有限公司 | 人miR-1249反义核酸及其应用 |
| CN102146412B (zh) * | 2011-01-11 | 2012-10-03 | 中山大学 | 一种小分子非编码RNA 基因hsa-miR-29b 及其应用 |
| US9045749B2 (en) | 2011-01-14 | 2015-06-02 | The General Hospital Corporation | Methods targeting miR-128 for regulating cholesterol/lipid metabolism |
| JP6211511B2 (ja) * | 2011-04-18 | 2017-10-11 | ディアミール, エルエルシーDiamir, Llc | miRNAに基づくユニバーサルスクリーニングテスト(UST) |
| EP2596136B1 (en) * | 2011-05-06 | 2014-07-16 | Zhongshan Hospital Fudan University | Marker consisting of plasma microrna and new method for diagnosis of hepatocellular carcinoma |
| US9771617B2 (en) * | 2011-06-27 | 2017-09-26 | Eisai R&D Management Co., Ltd. | Microrna biomarkers indicative of alzheimer's disease |
| CN102250904B (zh) * | 2011-07-13 | 2013-11-13 | 中国科学技术大学 | 一种预防和/或治疗黑色素瘤的药物 |
| EP2739754A4 (en) * | 2011-08-05 | 2015-01-21 | Univ Louisville Res Found | MICRO-RNA biomarkers |
| CN102433339B (zh) * | 2011-09-16 | 2012-10-03 | 山东莱博生物科技有限公司 | 一种HCV核酸适配体及其在制备HCV-cAg检测试剂盒中的应用 |
| CA2848999A1 (en) * | 2011-09-16 | 2013-03-21 | Lsip, Llc | Method for detecting bladder cancer cells, primer used in method for detecting bladder cancer cells, and bladder cancer marker |
| CA2850223A1 (en) | 2011-10-06 | 2013-04-11 | Eva Van Rooij | Control of whole body energy homeostasis by microrna regulation |
| WO2013056217A1 (en) | 2011-10-14 | 2013-04-18 | The Ohio State University | Methods and materials related to ovarian cancer |
| EP2584040A1 (en) * | 2011-10-17 | 2013-04-24 | Pharmahungary 2000 Kft. | Compounds for treatment of ischemic injury |
| CN104619353A (zh) * | 2011-12-13 | 2015-05-13 | 俄亥俄州国家创新基金会 | 与miR-21和miR-29a相关的方法和组合物、外切体抑制和癌症转移 |
| CN102533979A (zh) * | 2011-12-15 | 2012-07-04 | 苏州福英基因科技有限公司 | 各种癌症病理演变前期microrna-520水平原位杂交检测试剂盒及检测方法和应用 |
| ES2432853B1 (es) | 2011-12-15 | 2015-03-09 | Fundación Para La Investigación Biomédica Del Hospital Universitario Ramón Y Cajal | Metodo para el diagnostico y/o pronostico de dano renal agudo |
| WO2013110053A1 (en) | 2012-01-20 | 2013-07-25 | The Ohio State University | Breast cancer biomarker signatures for invasiveness and prognosis |
| WO2013155980A1 (zh) * | 2012-04-19 | 2013-10-24 | 中国科学院上海生命科学研究院 | 自身免疫性疾病相关的microRNA及其应用 |
| WO2013165320A1 (en) * | 2012-05-04 | 2013-11-07 | Agency For Science, Technology And Research | Treating cancer by increasing expression of socs6 |
| CN102676524B (zh) * | 2012-05-16 | 2013-04-03 | 北京旷博生物技术有限公司 | 乳腺癌分子标志物miR-147a |
| CN102676522B (zh) * | 2012-05-16 | 2013-04-10 | 北京旷博生物技术有限公司 | 乳腺癌分子标志物miR-195-5p |
| CN104685056A (zh) | 2012-06-21 | 2015-06-03 | 米拉根医疗股份有限公司 | 包含锁核酸基序的基于寡核苷酸的抑制剂 |
| CN102839173B (zh) * | 2012-08-24 | 2013-09-04 | 中国医科大学附属第一医院 | HIV感染疾病进展分子标志物miR-200c |
| CN102839172B (zh) * | 2012-08-24 | 2013-09-25 | 中国医科大学附属第一医院 | HIV感染疾病进展分子标志物miR-503 |
| ES2912033T3 (es) * | 2012-10-23 | 2022-05-24 | Caris Science Inc | Aptámeros y usos de los mismos |
| US10942184B2 (en) | 2012-10-23 | 2021-03-09 | Caris Science, Inc. | Aptamers and uses thereof |
| EP2733220B1 (en) | 2012-11-16 | 2017-10-18 | Siemens Aktiengesellschaft | Novel miRNA as a diagnostic marker for Alzheimer's Disease |
| CN104903468B (zh) | 2012-11-16 | 2019-04-23 | 萨尔大学 | 用于帕金森氏病的新诊断MiRNA标志物 |
| EP2733219B1 (en) * | 2012-11-16 | 2017-09-20 | Siemens Aktiengesellschaft | Diagnostic miRNA markers for Alzheimer |
| JP6441567B2 (ja) * | 2012-12-18 | 2018-12-19 | 三星電子株式会社Samsung Electronics Co.,Ltd. | 小胞内のポリヌクレオチドを含む乳がん診断用組成物及びキット、並びにそれを利用した乳がん診断方法 |
| EP2746406B1 (en) | 2012-12-18 | 2017-08-23 | Samsung Electronics Co., Ltd. | Composition and kit for diagnosing breast cancer including miRNAs within vesicle, and method of diagnosing breast cancer using the same |
| EP2935628B1 (en) | 2012-12-19 | 2018-03-21 | Caris Life Sciences Switzerland Holdings GmbH | Compositions and methods for aptamer screening |
| US9970012B2 (en) | 2013-03-14 | 2018-05-15 | Raadysan Biotech, Inc. | Replication factor C-40 (RFC40/RFC2) as a prognostic marker and target in estrogen positive and negative and triple negative breast cancer |
| US9193970B1 (en) * | 2013-03-14 | 2015-11-24 | Raadysan Biotech, Inc. | Replication factor C-40 (RFC40/RFC2) as a prognostic marker and target in estrogen positive and negative and triple negative breast cancer |
| US9546366B2 (en) | 2013-03-14 | 2017-01-17 | Raadysan Biotech, Inc. | Replication factor C-40 (RFC40/RFC2) as a prognostic marker and target in estrogen positive and negative and triple negative breast cancer |
| BR112015023275B1 (pt) * | 2013-03-15 | 2022-06-21 | Board Of Regents, The University Of Texas System | Método in vitro de detecção de biomarcador de câncer |
| WO2015020122A1 (ja) * | 2013-08-08 | 2015-02-12 | 国立大学法人 大阪大学 | 尿路上皮癌の診断または治療剤 |
| ES2813699T3 (es) | 2013-11-18 | 2021-03-24 | Diamir Llc | Métodos de uso de miARN de fluidos corporales para la detección y monitoreo de la enfermedad de Parkinson (PD) |
| CN103602747B (zh) * | 2013-11-28 | 2014-11-05 | 山东大学齐鲁医院 | 一种用于膀胱癌血清miRNA检测的内参及其检测引物与应用 |
| US10815527B2 (en) * | 2013-12-19 | 2020-10-27 | Hummingbird Diagnostics Gmbh | Determination of platelet-miRNAs in alzheimer's disease |
| US9758773B2 (en) | 2014-02-14 | 2017-09-12 | Agilent Technologies, Inc. | Thermostable type-A DNA polymerase mutant with increased resistance to inhibitors in blood |
| JP6415829B2 (ja) * | 2014-02-28 | 2018-10-31 | キヤノンメディカルシステムズ株式会社 | 検体に含まれるエキソソームの特性を判定する方法、診断方法および装置 |
| CN107236792B (zh) * | 2014-08-15 | 2020-12-04 | 深圳市晋百慧生物有限公司 | 用于检测肠癌的标记物及其应用 |
| JP2018503646A (ja) | 2015-01-20 | 2018-02-08 | ミラゲン セラピューティクス, インコーポレイテッド | miR−92阻害剤およびその使用 |
| US10400288B2 (en) | 2015-02-11 | 2019-09-03 | Aarhus Universitet | MicroRNA-based method for early detection of prostate cancer in urine samples |
| JP6769979B2 (ja) | 2015-02-27 | 2020-10-14 | キアゲン ゲゼルシャフト ミット ベシュレンクテル ハフツング | マイクロrnaに基づく前立腺がん患者の予後評価方法 |
| ES2741591T3 (es) * | 2015-03-13 | 2020-02-11 | Univ Hiroshima | Método para ayudar a la detección de la enfermedad de Alzheimer o una discapacidad cognitiva moderada |
| IL256634B2 (en) | 2015-06-29 | 2025-08-01 | Caris Science Inc | Therapeutic oligonucleotides |
| US10941176B2 (en) | 2015-07-28 | 2021-03-09 | Caris Science, Inc. | Therapeutic oligonucleotides |
| KR102601499B1 (ko) * | 2015-11-06 | 2023-11-13 | 연세대학교 산학협력단 | miRNA 발현 수준으로부터 UQCRB 관련 질병을 진단하는 방법 |
| ITUB20155765A1 (it) * | 2015-11-20 | 2017-05-20 | Braindtech S R L | Metodi per diagnosi, prognosi e monitoraggio terapeutico di patologie neurologiche, neurodegenerative e infiammatorie basati su microRNA contenuto in microvescicole microglia |
| CN106729750A (zh) * | 2015-11-20 | 2017-05-31 | 昆山彭济凯丰生物科技有限公司 | 通过miR-183治疗高血脂、脂肪肝、二型糖尿病和降低体重的方法和药物及它们的应用 |
| CN105400785A (zh) * | 2015-12-01 | 2016-03-16 | 中国人民解放军第四军医大学 | 一种与他莫昔芬耐药性相关的microRNA分子MiR-200a及其应用 |
| CN105586401A (zh) * | 2015-12-14 | 2016-05-18 | 常州杰傲医学检验所有限公司 | 一种用于乳腺癌诊断的miRNA标志物、其应用及诊断试剂盒 |
| US10975436B2 (en) | 2016-01-05 | 2021-04-13 | Diamir, Llc | Methods of using miRNA from bodily fluids for diagnosis and monitoring of neurodevelopmental disorders |
| CN105567826A (zh) * | 2016-01-25 | 2016-05-11 | 苏州大学附属第二医院 | has-miR-29b-3p的检测 |
| EP4339288A3 (en) | 2016-03-18 | 2024-06-05 | Caris Science, Inc. | Oligonucleotide probes and uses thereof |
| ES2993025T3 (en) | 2016-03-21 | 2024-12-20 | Diamir Llc | Methods of using mirnas from bodily fluids for detection and differentiation of neurodegenerative diseases |
| CN105755123A (zh) * | 2016-03-21 | 2016-07-13 | 河北医科大学第医院 | 一种结直肠癌相关的标志物、其引物及应用 |
| CN105603113B (zh) * | 2016-03-30 | 2019-02-05 | 江苏省疾病预防控制中心 | HIV-1早期感染相关的血清miRNA在制备试剂盒中的应用 |
| US11293017B2 (en) | 2016-05-25 | 2022-04-05 | Caris Science, Inc. | Oligonucleotide probes and uses thereof |
| CN106282360B (zh) * | 2016-08-31 | 2019-11-08 | 武汉博杰生物医学科技有限公司 | 一种用于结肠癌预测转移的血浆miRNA组合、其探针组合物及应用 |
| US11236337B2 (en) | 2016-11-01 | 2022-02-01 | The Research Foundation For The State University Of New York | 5-halouracil-modified microRNAs and their use in the treatment of cancer |
| US11584932B2 (en) | 2016-11-01 | 2023-02-21 | The Research Foundation For The State University Of New York | 5-halouracil-modified microRNAs and their use in the treatment of cancer |
| CN108938659A (zh) * | 2017-05-19 | 2018-12-07 | 昆山彭济凯丰生物科技有限公司 | 一种调节食欲和体重的方法和药物及它们的应用 |
| WO2019008415A1 (en) * | 2017-07-05 | 2019-01-10 | Datar Rajan | EXOSOMED AND PBMC GENE EXPRESSION ANALYSIS FOR CANCER CARE |
| US10781487B2 (en) | 2017-07-24 | 2020-09-22 | Diamir, Llc | miRNA-based methods for detecting and monitoring aging |
| CN107557467B (zh) * | 2017-08-10 | 2021-05-11 | 李永东 | 一种与脑动脉瘤相关的临床标志物及其应用 |
| JP2019050772A (ja) * | 2017-09-15 | 2019-04-04 | 国立大学法人 熊本大学 | ワクチン接種後副反応を予測する検査方法 |
| CN111601890B (zh) * | 2017-12-14 | 2024-04-19 | 联合细胞Ev股份公司 | 用于治疗肾癌的包含miRNA的药物载体 |
| WO2019144183A1 (en) * | 2018-01-23 | 2019-08-01 | La Trobe University | Biomarkers of colorectal cancer |
| CN108295268A (zh) * | 2018-02-05 | 2018-07-20 | 苏州吉玛基因股份有限公司 | 与肝癌诊疗相关的血清外泌体hsa-miR-93及其应用 |
| WO2019159884A1 (ja) | 2018-02-13 | 2019-08-22 | 東レ株式会社 | 認知症の検出のためのキット又はデバイス及び方法 |
| CN108103202A (zh) * | 2018-03-06 | 2018-06-01 | 苏州吉玛基因股份有限公司 | 与肝癌诊疗相关的7种血清外泌体miRNAs及其应用 |
| CN108546752B (zh) * | 2018-04-20 | 2021-08-31 | 南京医科大学 | 检测和/或预测人类巨大儿的miRNA标志物或其组合及其应用 |
| CN108676880B (zh) * | 2018-05-24 | 2021-01-01 | 武汉大学 | 一种人血清afp阴性肝细胞癌检测试剂盒 |
| CN109022587B (zh) * | 2018-09-13 | 2022-02-22 | 南京求臻基因科技有限公司 | 一种急性髓系白血病的miRNA标志物及其应用 |
| KR102131519B1 (ko) * | 2018-10-30 | 2020-07-07 | 가톨릭대학교 산학협력단 | 간암 전이 진단 또는 예후 예측용 바이오마커 및 이의 용도 |
| KR102256747B1 (ko) * | 2018-10-30 | 2021-05-25 | 가톨릭대학교 산학협력단 | 엑소좀 miR-125b를 포함하는 간암 전이 진단 또는 예측용 바이오마커 및 이의 용도 |
| CN109295001A (zh) * | 2018-11-14 | 2019-02-01 | 姜大奎 | 一种脐带间充质干细胞复合外泌体及其制备方法 |
| EP3899052A4 (en) * | 2018-12-19 | 2023-02-22 | Agency For Science, Technology And Research | PROCESS |
| CN109971851A (zh) * | 2019-01-22 | 2019-07-05 | 宁波大学 | MiR-125b-2-3p作为鉴别诊断肾癌亚型的分子标志物及其在肿瘤转移中的用途 |
| CN109777874B (zh) * | 2019-01-29 | 2022-11-11 | 上海长海医院 | 一种适用于胰腺导管腺癌诊断及预后判断的血浆外泌体miRNA标志物及应用 |
| CN109837343B (zh) * | 2019-02-22 | 2024-03-01 | 中国科学院北京基因组研究所 | 早期肺腺癌特异性外泌体miRNA及其应用 |
| US10900091B2 (en) * | 2019-03-15 | 2021-01-26 | The Chinese University Of Hong Kong | miR-133a as a marker for colorectal cancer |
| CN110699450A (zh) * | 2019-05-22 | 2020-01-17 | 璞晞(广州)生物免疫技术有限公司 | miRNA生物标志物在肝脏疾病诊断和预后判断中的应用 |
| CN110791560B (zh) * | 2019-11-06 | 2021-09-14 | 中国医学科学院医药生物技术研究所 | 一种用于阿尔茨海默病诊断和/或治疗的miRNA标志物 |
| US12091716B2 (en) | 2020-01-15 | 2024-09-17 | Mayo Foundation For Medical Education And Research | Methods and materials for identifying and treating traumatic brain injury |
| ES2856232B2 (es) * | 2020-03-25 | 2023-11-24 | Fundacion Para La Investig Biomedica Del Hospital 12 De Octubre | Biomarcadores para predecir la respuesta de un sujeto a una terapia con bcg, metodos y usos basados en los mismos |
| US20230407401A1 (en) * | 2020-05-29 | 2023-12-21 | National Institute Of Immunology | Dna damage dependent microrna signature for cancers, methods and uses related thereto |
| WO2021262919A2 (en) | 2020-06-26 | 2021-12-30 | The Research Foundation For The State University Of New York | 5-halouracil-modified micrornas and their use in the treatment of cancer |
| US20220017971A1 (en) * | 2020-07-15 | 2022-01-20 | Kookmin University Industry Academy Cooperation Foundation | Composition for predicting chemotherapy resistance of ovarian cancer and use thereof |
| CN112494654B (zh) * | 2020-12-10 | 2021-12-17 | 暨南大学附属第一医院(广州华侨医院) | 包含LncRNA HCG18抑制剂的药物组合物及其应用 |
| CN114469996B (zh) * | 2021-12-23 | 2023-10-20 | 中国医学科学院医学生物学研究所 | 一种包含miR-135b-5p的外泌体及在抗轮状病毒感染中的应用 |
| CN115192710A (zh) * | 2022-05-27 | 2022-10-18 | 华南理工大学 | 一种miRNA-200s保护剂在制备神经系统疾病类药物中的应用和药物及模型构建方法 |
| CN117802232B (zh) * | 2022-09-30 | 2025-08-19 | 上海思路迪生物医学科技有限公司 | 血液小型胞外囊泡miRNA在卵巢癌诊断中的应用 |
| CN115944646A (zh) * | 2022-12-30 | 2023-04-11 | 南华大学附属第一医院 | MicroRNA-32-5p在制备抗骨质疏松症药物中的应用 |
| ES2981248A1 (es) * | 2023-03-09 | 2024-10-07 | Servicio Andaluz De Salud | Panel de microARNs como biomarcadores de alopecia areata severa |
| CN117821577B (zh) * | 2023-10-19 | 2024-08-23 | 长沙干细胞与再生医学工业技术研究院有限公司 | 一种有效治疗卵巢功能减退的羊膜间充质干细胞的筛选方法 |
| CN119524005B (zh) * | 2024-11-14 | 2025-07-25 | 广东医科大学附属医院 | 干预B细胞miR-330-5p在制备治疗SLE药物中的应用 |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005121369A2 (en) * | 2004-06-02 | 2005-12-22 | Sourcepharm, Inc. | Rna-containing microvesicles and methods therefor |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5089181A (en) * | 1987-02-24 | 1992-02-18 | Vestar, Inc. | Method of dehydrating vesicle preparations for long term storage |
| US6812023B1 (en) * | 2000-04-27 | 2004-11-02 | Anosys, Inc. | Methods of producing membrane vesicles |
| US20050159378A1 (en) * | 2001-05-18 | 2005-07-21 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of Myc and/or Myb gene expression using short interfering nucleic acid (siNA) |
| US20030036077A1 (en) * | 2001-05-31 | 2003-02-20 | Chronix Biomedical | Methods for generating an mRNA expression profile from an ecellular mRNA containing blood sample and using the same to identify functional state markers |
| WO2002099077A2 (en) * | 2001-06-06 | 2002-12-12 | Proteologics, Inc. | Methods and compositions related to tagging of membrane surface proteins |
| US20070077553A1 (en) * | 2003-10-30 | 2007-04-05 | Rosetta Genomics | Bioinformatically detectable group of novel vaccinia regulatory genes and uses thereof |
| US20050064470A1 (en) * | 2003-07-17 | 2005-03-24 | Rana Tariq M. | High throughput screening methods for identifying RNA binding compounds |
| CA2566662C (en) * | 2004-05-13 | 2013-05-07 | Centre National De La Recherche Scientifique | A device for binding a target entity to a bait entity and detection methods using the same |
| US7888010B2 (en) * | 2004-05-28 | 2011-02-15 | Asuragen, Inc. | Methods and compositions involving microRNA |
| US8021847B2 (en) * | 2004-06-02 | 2011-09-20 | Proxy Life Science Holdings, Inc. | Microvesicle-based compositions and methods |
| WO2006102687A1 (en) * | 2005-03-22 | 2006-09-28 | The Trustees Of Columbia University In The City Of New York | A liposome-based microarray and methods of use thereof |
| ES2508893T3 (es) | 2006-01-05 | 2014-10-16 | The Ohio State University Research Foundation | Métodos basados en microARN para el diagnóstico de cánceres de estómago |
| WO2007084962A2 (en) * | 2006-01-18 | 2007-07-26 | The Regents Of The University Of California | A fluid membrane-based ligand display system for live cell assays and disease diagnosis applications |
| WO2009015357A1 (en) * | 2007-07-25 | 2009-01-29 | University Of Louisville Research Foundation, Inc. | Exosome-associated microrna as a diagnostic marker |
| BRPI0816852A2 (pt) | 2007-09-14 | 2017-06-06 | Univ Ohio State Res Found | método de diagnosticar ou prognosticar doença ou desordem em sujeito, biomarcador, método para determinar e/ou prever se sujeito tem diferenciação da desordem, biomarcador para desordem pulmonar, método para diagnosticar se sujeito tem, ou está em risco de desenvolver doença ou desordem, método de inibir a proliferação de doença ou desordem e método para identificar agente terapêutico do câncer. |
| CN102301002A (zh) * | 2008-11-12 | 2011-12-28 | 卡里斯生命科学卢森堡控股有限责任公司 | 使用外来体来确定表现型的方法和系统 |
-
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- 2015-01-21 IL IL236831A patent/IL236831A0/en unknown
-
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005121369A2 (en) * | 2004-06-02 | 2005-12-22 | Sourcepharm, Inc. | Rna-containing microvesicles and methods therefor |
Non-Patent Citations (2)
| Title |
|---|
| EBANDRÉS等: "Identification by Real-time PCR of 13 mature microRNAs differentially expressed in colorectal cancer and non-tumoral tissues", 《MOL CANCER》 * |
| HADI VALADI等: "Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells", 《NATURE CELL BIOLOGY》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109295213A (zh) * | 2018-11-01 | 2019-02-01 | 中国人民解放军第00医院 | 免疫性血小板减少症的miRNA标志物及试剂盒和应用 |
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| JP2010538653A (ja) | 2010-12-16 |
| CN101842484B (zh) | 2015-07-29 |
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| US8455199B2 (en) | 2013-06-04 |
| EP2610342A1 (en) | 2013-07-03 |
| EP2190992A4 (en) | 2011-02-23 |
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| US20100279292A1 (en) | 2010-11-04 |
| EP2190992A1 (en) | 2010-06-02 |
| AU2008298744B2 (en) | 2014-09-04 |
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