CA2747398C - Generation and maintenance of stem cells - Google Patents

Generation and maintenance of stem cells Download PDF

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CA2747398C
CA2747398C CA2747398A CA2747398A CA2747398C CA 2747398 C CA2747398 C CA 2747398C CA 2747398 A CA2747398 A CA 2747398A CA 2747398 A CA2747398 A CA 2747398A CA 2747398 C CA2747398 C CA 2747398C
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cells
cell
inhibitor
pluripotent
therapeutic composition
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CA2747398A1 (en
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Wenlin Li
Hongyan Zhou
Sheng Ding
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Scripps Research Institute
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Scripps Research Institute
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
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    • C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0607—Non-embryonic pluripotent stem cells, e.g. MASC
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2306—Interleukin-6 (IL-6)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/602—Sox-2
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/604—Klf-4
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
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    • C12N2510/00—Genetically modified cells

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  • Health & Medical Sciences (AREA)
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  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Transplantation (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2747398A 2008-12-17 2009-12-16 Generation and maintenance of stem cells Active CA2747398C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US13840708P 2008-12-17 2008-12-17
US61/138,407 2008-12-17
PCT/US2009/068274 WO2010077955A1 (en) 2008-12-17 2009-12-16 Generation and maintenance of stem cells

Publications (2)

Publication Number Publication Date
CA2747398A1 CA2747398A1 (en) 2010-07-08
CA2747398C true CA2747398C (en) 2023-06-20

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US (5) US8906677B2 (enExample)
EP (2) EP2373784B1 (enExample)
JP (6) JP6093110B2 (enExample)
KR (2) KR101909847B1 (enExample)
CN (2) CN102317442B (enExample)
AU (3) AU2009333259B2 (enExample)
BR (1) BRPI0922572A2 (enExample)
CA (1) CA2747398C (enExample)
ES (1) ES2645869T3 (enExample)
HK (1) HK1199469A1 (enExample)
IL (1) IL213582A0 (enExample)
NO (1) NO2373784T3 (enExample)
SG (1) SG172160A1 (enExample)
WO (1) WO2010077955A1 (enExample)

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