JP6093110B2 - 幹細胞の作製と維持 - Google Patents

幹細胞の作製と維持 Download PDF

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JP6093110B2
JP6093110B2 JP2011542403A JP2011542403A JP6093110B2 JP 6093110 B2 JP6093110 B2 JP 6093110B2 JP 2011542403 A JP2011542403 A JP 2011542403A JP 2011542403 A JP2011542403 A JP 2011542403A JP 6093110 B2 JP6093110 B2 JP 6093110B2
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cell
cells
inhibitor
pluripotent
culture
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JP2012511935A (ja
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ウェンリン リー
ウェンリン リー
ホンヤン チョウ
ホンヤン チョウ
シェン ディン
シェン ディン
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ザ スクリプス リサーチ インスティテュート
ザ スクリプス リサーチ インスティテュート
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  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
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JP2011542403A 2008-12-17 2009-12-16 幹細胞の作製と維持 Active JP6093110B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US13840708P 2008-12-17 2008-12-17
US61/138,407 2008-12-17
PCT/US2009/068274 WO2010077955A1 (en) 2008-12-17 2009-12-16 Generation and maintenance of stem cells

Related Child Applications (1)

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JP2015179265A Division JP2016052305A (ja) 2008-12-17 2015-09-11 幹細胞の作製と維持

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JP2012511935A JP2012511935A (ja) 2012-05-31
JP6093110B2 true JP6093110B2 (ja) 2017-03-08

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JP2011542403A Active JP6093110B2 (ja) 2008-12-17 2009-12-16 幹細胞の作製と維持
JP2015179265A Pending JP2016052305A (ja) 2008-12-17 2015-09-11 幹細胞の作製と維持
JP2017027509A Active JP6683639B2 (ja) 2008-12-17 2017-02-17 幹細胞の作製と維持
JP2020005849A Pending JP2020054403A (ja) 2008-12-17 2020-01-17 幹細胞の作製と維持
JP2022079999A Pending JP2022105220A (ja) 2008-12-17 2022-05-16 幹細胞の作製と維持
JP2024081065A Pending JP2024098047A (ja) 2008-12-17 2024-05-17 幹細胞の作製と維持

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JP2017027509A Active JP6683639B2 (ja) 2008-12-17 2017-02-17 幹細胞の作製と維持
JP2020005849A Pending JP2020054403A (ja) 2008-12-17 2020-01-17 幹細胞の作製と維持
JP2022079999A Pending JP2022105220A (ja) 2008-12-17 2022-05-16 幹細胞の作製と維持
JP2024081065A Pending JP2024098047A (ja) 2008-12-17 2024-05-17 幹細胞の作製と維持

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US (5) US8906677B2 (enExample)
EP (2) EP2373784B1 (enExample)
JP (6) JP6093110B2 (enExample)
KR (2) KR101909847B1 (enExample)
CN (2) CN102317442B (enExample)
AU (3) AU2009333259B2 (enExample)
BR (1) BRPI0922572A2 (enExample)
CA (1) CA2747398C (enExample)
ES (1) ES2645869T3 (enExample)
HK (1) HK1199469A1 (enExample)
IL (1) IL213582A0 (enExample)
NO (1) NO2373784T3 (enExample)
SG (1) SG172160A1 (enExample)
WO (1) WO2010077955A1 (enExample)

Families Citing this family (53)

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Publication number Priority date Publication date Assignee Title
WO2009117439A2 (en) 2008-03-17 2009-09-24 The Scripps Research Institute Combined chemical and genetic approaches for generation of induced pluripotent stem cells
SG172160A1 (en) 2008-12-17 2011-07-28 Scripps Research Inst Generation and maintenance of stem cells
EP2412800A1 (en) 2010-07-29 2012-02-01 Koninklijke Nederlandse Akademie van Wetenschappen Liver organoid, uses thereof and culture method for obtaining them
MX2011008044A (es) 2009-02-03 2012-01-12 Koninklijke Nederlandse Akademie Van Wetenschappen Medio de cultivo para celulas madre epiteliales y organoides que comprenden las celulas madre.
US9752124B2 (en) 2009-02-03 2017-09-05 Koninklijke Nederlandse Akademie Van Wetenschappen Culture medium for epithelial stem cells and organoids comprising the stem cells
CN102575251B (zh) * 2009-05-18 2018-12-04 库尔纳公司 通过抑制针对重编程因子的天然反义转录物来治疗重编程因子相关的疾病
CA3091210C (en) 2009-10-16 2023-04-04 The Scripps Research Institute Induction of pluripotent cells
AU2010315166B2 (en) 2009-11-04 2015-01-22 FUJIFILM Cellular Dynamics, Inc. Episomal reprogramming with chemicals
ES2893699T3 (es) 2010-03-31 2022-02-09 Scripps Research Inst Reprogramación de células
US9376664B2 (en) 2010-06-14 2016-06-28 The Scripps Research Institute Reprogramming of cells to a new fate
US9144585B2 (en) 2010-07-27 2015-09-29 Technion Research & Development Foundation Limited Isolated mesenchymal progenitor cells and extracellular matrix produced thereby
WO2012014207A2 (en) * 2010-07-27 2012-02-02 Technion Research & Development Foundation Ltd. Method for generating induced pluripotent stem cells from keratinocytes derived from plucked hair follicles
US9447408B2 (en) 2010-09-14 2016-09-20 Kyoto University Method of efficiently establishing induced pluripotent stem cells
WO2012044979A2 (en) 2010-10-01 2012-04-05 The Goverment Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Manipulation of stem cell function by p53 isoforms
KR20140063501A (ko) 2010-12-22 2014-05-27 페이트 세러퓨틱스, 인코포레이티드 단세포 분류 및 iPSC의 증강된 재프로그래밍을 위한 세포 배양 플랫폼
US10428309B2 (en) 2011-12-01 2019-10-01 New York Stem Cell Foundation, Inc. Systems and methods for producing stem cells and differentiated cells
BR112014013152B1 (pt) 2011-12-01 2021-08-31 New York Stem Cell Foundation, Inc Sistema automatizado para geração de células-tronco pluripotentes induzidas (ipscs)
AU2013290146B2 (en) * 2012-07-11 2018-01-18 Imstem Biotechnology, Inc. Mesenchymal-like stem cells derived from human embryonic stem cells, methods and uses thereof
EP2958990B1 (en) 2013-02-20 2019-10-16 Regeneron Pharmaceuticals, Inc. Genetic modification of rats
US20150037883A1 (en) * 2013-03-03 2015-02-05 Royan Institute Method for derivation and long-term establishment of ground state pluripotent embryonic stem cells
KR20200136508A (ko) 2013-04-16 2020-12-07 리제너론 파마슈티칼스 인코포레이티드 랫트 게놈의 표적화된 변형
CN105531365A (zh) * 2013-04-23 2016-04-27 耶达研究及发展有限公司 分离的幼稚多能干细胞和产生所述细胞的方法
BR112015031094A2 (pt) 2013-06-14 2017-07-25 Univ Queensland células progenitoras renais
KR101655383B1 (ko) 2013-07-27 2016-09-08 고려대학교 산학협력단 소분자 화합물을 포함하는 만능성 줄기세포의 염색체 안정성 유지용 조성물
US9512406B2 (en) 2013-12-20 2016-12-06 The J. David Gladstone Institute, a testamentary trust established under the Will of J. David Gladstone Generating hepatocytes
ES2966757T3 (es) 2014-03-04 2024-04-24 Fate Therapeutics Inc Métodos de reprogramación mejorados y plataformas de cultivo celular
EP3129466B1 (en) * 2014-04-07 2020-08-05 Memorial Sloan Kettering Cancer Center Modulating cell proliferation and pluripotency
CN108064274A (zh) * 2014-07-30 2018-05-22 耶达研究及发展有限公司 用于培养多能干细胞的培养基
US10894948B2 (en) 2014-08-22 2021-01-19 Cambridge Enterprise Limited Resetting pluripotent stem cells
CN105441384B (zh) * 2014-09-26 2021-01-05 北京大学 一种制备动物和人类原始多潜能干细胞的方法，试剂盒及用途
AU2016211688A1 (en) 2015-01-26 2017-08-10 Fate Therapeutics, Inc. Cells with increased immuno-regulatory properties and methods for their use and manufacture
EP3313420B1 (en) * 2015-06-25 2024-03-13 The Children's Medical Center Corporation Methods and compositions relating to hematopoietic stem cell expansion, enrichment, and maintenance
RU2018106515A (ru) 2015-07-21 2019-08-21 Зе Чилдрен'С Медикал Сентер Корпорейшн Pd-l1-экспрессирующие гемопоэтические стволовые клетки и применения
SG10202111851YA (en) 2015-10-16 2021-12-30 Fate Therapeutics Inc Platform for the Induction & Maintenance of Ground State Pluripotency
HK1256552A1 (zh) * 2015-10-21 2019-09-27 印第安纳大学研究与技术公司 生成人内耳感觉上皮和感觉神经元的方法
JP6937036B2 (ja) 2016-03-08 2021-09-22 公立大学法人名古屋市立大学 人工多能性幹細胞の腸管上皮細胞への分化誘導
AU2017235461B2 (en) 2016-03-15 2023-02-23 Children's Medical Center Corporation Methods and compositions relating to hematopoietic stem cell expansion
US11352605B2 (en) 2016-05-12 2022-06-07 Erasmus University Medical Center Rotterdam Method for culturing myogenic cells, cultures obtained therefrom, screening methods, and cell culture medium
DK3519558T3 (da) 2016-09-28 2023-11-13 Organovo Inc Anvendelse af modificeret nyrevæv i assays
CN109890956A (zh) * 2016-10-28 2019-06-14 国立研究开发法人国立癌研究中心 人肝前体细胞的制备方法
JP2018091790A (ja) * 2016-12-06 2018-06-14 東ソー株式会社 細胞の染色方法
CA3062781A1 (en) * 2017-05-08 2018-11-15 Regents Of The University Of Minnesota Acceleration of stem cell differentiation
NL2019517B1 (en) 2017-09-08 2019-03-19 Univ Erasmus Med Ct Rotterdam New therapy for Pompe disease
WO2019060708A1 (en) 2017-09-22 2019-03-28 The Children's Medical Center Corporation TREATMENT OF TYPE 1 DIABETES AND AUTOIMMUNE DISEASES OR DISORDERS
KR20200091885A (ko) 2017-11-30 2020-07-31 고쿠리츠 다이가쿠 호진 교토 다이가쿠 세포의 배양 방법
WO2019151386A1 (ja) * 2018-01-31 2019-08-08 富士フイルム株式会社 細胞の製造方法
JP7317317B2 (ja) 2018-02-09 2023-07-31 公立大学法人名古屋市立大学 多能性幹細胞から腸管上皮細胞への分化誘導方法
US11901045B2 (en) * 2019-01-15 2024-02-13 International Business Machines Corporation Machine learning framework for finding materials with desired properties
KR102224273B1 (ko) * 2019-10-10 2021-03-08 고려대학교 산학협력단 줄기세포 유래 성숙 심근세포 및 이를 이용한 심혈관 질환 모델
EP3922431A1 (en) 2020-06-08 2021-12-15 Erasmus University Medical Center Rotterdam Method of manufacturing microdevices for lab-on-chip applications
CN112063656A (zh) * 2020-08-28 2020-12-11 中国科学院广州生物医药与健康研究院 Map2k3或Map2k6在提高诱导成体细胞生成多能性干细胞效率中的用途
WO2024186913A2 (en) * 2023-03-07 2024-09-12 The Board Of Regents Of The University Of Texas System Compositions and methods for facilitating interspecies chimerism
WO2024243481A1 (en) * 2023-05-24 2024-11-28 University Of Connecticut Compositions and methods for generation of bovine pluripotent embryonic stem cells

Family Cites Families (121)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4797368A (en) 1985-03-15 1989-01-10 The United States Of America As Represented By The Department Of Health And Human Services Adeno-associated virus as eukaryotic expression vector
US5506337A (en) 1985-03-15 1996-04-09 Antivirals Inc. Morpholino-subunit combinatorial library and method
US5139941A (en) 1985-10-31 1992-08-18 University Of Florida Research Foundation, Inc. AAV transduction vectors
US5010175A (en) 1988-05-02 1991-04-23 The Regents Of The University Of California General method for producing and selecting peptides with specific properties
US5703055A (en) 1989-03-21 1997-12-30 Wisconsin Alumni Research Foundation Generation of antibodies through lipid mediated DNA delivery
IE66205B1 (en) 1990-06-14 1995-12-13 Paul A Bartlett Polypeptide analogs
US5650489A (en) 1990-07-02 1997-07-22 The Arizona Board Of Regents Random bio-oligomer library, a method of synthesis thereof, and a method of use thereof
US5384253A (en) 1990-12-28 1995-01-24 Dekalb Genetics Corporation Genetic transformation of maize cells by electroporation of cells pretreated with pectin degrading enzymes
WO1993004169A1 (en) 1991-08-20 1993-03-04 Genpharm International, Inc. Gene targeting in animal cells using isogenic dna constructs
US5573905A (en) 1992-03-30 1996-11-12 The Scripps Research Institute Encoded combinatorial chemical libraries
US5340740A (en) 1992-05-15 1994-08-23 North Carolina State University Method of producing an avian embryonic stem cell culture and the avian embryonic stem cell culture produced by the process
US5702932A (en) 1992-07-20 1997-12-30 University Of Florida Microinjection methods to transform arthropods with exogenous DNA
US5288514A (en) 1992-09-14 1994-02-22 The Regents Of The University Of California Solid phase and combinatorial synthesis of benzodiazepine compounds on a solid support
US5824837A (en) 1993-07-22 1998-10-20 Merck & Co., Inc. Expression of human interleukin-1β in a transgenic animal
US5519134A (en) 1994-01-11 1996-05-21 Isis Pharmaceuticals, Inc. Pyrrolidine-containing monomers and oligomers
US5593853A (en) 1994-02-09 1997-01-14 Martek Corporation Generation and screening of synthetic drug libraries
US5539083A (en) 1994-02-23 1996-07-23 Isis Pharmaceuticals, Inc. Peptide nucleic acid combinatorial libraries and improved methods of synthesis
US5656610A (en) 1994-06-21 1997-08-12 University Of Southern California Producing a protein in a mammal by injection of a DNA-sequence into the tongue
US5525735A (en) 1994-06-22 1996-06-11 Affymax Technologies Nv Methods for synthesizing diverse collections of pyrrolidine compounds
US5549974A (en) 1994-06-23 1996-08-27 Affymax Technologies Nv Methods for the solid phase synthesis of thiazolidinones, metathiazanones, and derivatives thereof
US5736524A (en) 1994-11-14 1998-04-07 Merck & Co.,. Inc. Polynucleotide tuberculosis vaccine
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
US5569588A (en) 1995-08-09 1996-10-29 The Regents Of The University Of California Methods for drug screening
US6013516A (en) 1995-10-06 2000-01-11 The Salk Institute For Biological Studies Vector and method of use for nucleic acid delivery to non-dividing cells
US5780448A (en) 1995-11-07 1998-07-14 Ottawa Civic Hospital Loeb Research DNA-based vaccination of fish
GB9807935D0 (en) 1998-04-14 1998-06-10 Univ Edinburgh Lineage specific cells and progenitor cells
US5945100A (en) 1996-07-31 1999-08-31 Fbp Corporation Tumor delivery vehicles
US5981274A (en) 1996-09-18 1999-11-09 Tyrrell; D. Lorne J. Recombinant hepatitis virus vectors
US6090622A (en) 1997-03-31 2000-07-18 The Johns Hopkins School Of Medicine Human embryonic pluripotent germ cells
US6087496A (en) 1998-05-22 2000-07-11 G. D. Searle & Co. Substituted pyrazoles suitable as p38 kinase inhibitors
JP4344960B2 (ja) 1997-05-23 2009-10-14 バイエル、コーポレイション アリール尿素によるｐ３８キナーゼ活性の阻害
US6344476B1 (en) 1997-05-23 2002-02-05 Bayer Corporation Inhibition of p38 kinase activity by aryl ureas
US6093742A (en) 1997-06-27 2000-07-25 Vertex Pharmaceuticals, Inc. Inhibitors of p38
US5994624A (en) 1997-10-20 1999-11-30 Cotton Incorporated In planta method for the production of transgenic plants
TR200001079T2 (tr) 1997-10-20 2000-07-21 F.Hoffmann-La Roche Ag Bisiklik kinaz inhibitörleri.
WO1999027076A1 (en) 1997-11-25 1999-06-03 Arc Genomic Research Pluripotent embryonic stem cells and methods of obtaining them
US5994136A (en) 1997-12-12 1999-11-30 Cell Genesys, Inc. Method and means for producing high titer, safe, recombinant lentivirus vectors
JP2001526222A (ja) 1997-12-22 2001-12-18 バイエル コーポレイション アリールおよびヘテロアリール置換複素環尿素を使用するｐ３８キナーゼ活性の阻害
DE69830513T2 (de) 1997-12-22 2006-03-16 Bayer Pharmaceuticals Corp., West Haven HEMMUNG DER p38 KINASE UNTER VERWENDUNG VON SYMMETRISCHEN UND ASYMMETRISCHEN DIPHENYLHARNSTOFFEN
MY132496A (en) 1998-05-11 2007-10-31 Vertex Pharma Inhibitors of p38
CA2336220A1 (en) * 1998-07-24 2000-02-03 The Carnegie Institution Of Washington Method for maintenance and propagation of germline stem cells using members of the tgf-.beta. family of growth factors
DE69917296T2 (de) 1998-08-20 2005-05-25 Smithkline Beecham Corp. Neue substituierte triazolverbindungen
US6184226B1 (en) 1998-08-28 2001-02-06 Scios Inc. Quinazoline derivatives as inhibitors of P-38 α
AR023659A1 (es) 1998-09-18 2002-09-04 Vertex Pharma Un compuesto inhibidor de p38, una composicion farmaceutica que lo comprende y el uso de dicha composicion en el tratamiento y prevencion de estados patologicos
JP2002526482A (ja) 1998-09-18 2002-08-20 バーテックス ファーマシューティカルズ インコーポレイテッド ｐ３８のインヒビター
WO2000027995A1 (en) 1998-11-09 2000-05-18 Monash University Embryonic stem cells
US6509361B1 (en) 1999-05-12 2003-01-21 Pharmacia Corporation 1,5-Diaryl substituted pyrazoles as p38 kinase inhibitors
US6541477B2 (en) 1999-08-27 2003-04-01 Scios, Inc. Inhibitors of p38-a kinase
US20020142457A1 (en) 1999-12-28 2002-10-03 Akihiro Umezawa Cell having the potentiality of differentiation into cardiomyocytes
US6560871B1 (en) 2000-03-21 2003-05-13 Hewlett-Packard Development Company, L.P. Semiconductor substrate having increased facture strength and method of forming the same
US6458589B1 (en) * 2000-04-27 2002-10-01 Geron Corporation Hepatocyte lineage cells derived from pluripotent stem cells
JP2008119003A (ja) * 2001-10-30 2008-05-29 Renomedix Institute Inc 中胚葉幹細胞もしくはｅｓ細胞、または不死化した中胚葉幹細胞から神経系細胞への分化誘導方法
GB2398795A (en) * 2001-12-28 2004-09-01 Cellartis Ab A method for the establishment of a pluripotent human blastocyst-derived stem cell line
GB0210539D0 (en) * 2002-05-08 2002-06-19 Univ Edinburgh Control of es cell self renewal and lineage specification, and medium therefor
AU2003286442A1 (en) 2002-10-16 2004-05-04 Advanced Cell Technology, Inc. Methods using gene trapped stem cells for marking pathways of stem cell differentiation and making and isolating differentiated cells
AU2004212490B2 (en) 2003-02-10 2008-05-15 Amgen Inc. Vanilloid receptor ligands and their use in treatments
AU2003901099A0 (en) 2003-03-11 2003-03-27 Es Cell International Pte Ltd. Methods of inducing differentiation of stem cells
US20070010008A1 (en) * 2005-06-29 2007-01-11 Tissuetech, Inc. Ex vivo expansion of primary animal cells
RU2392315C2 (ru) 2003-10-03 2010-06-20 Кейити ФУКУДА Способ индукции дифференциации стволовых клеток в миокардиальные
EP1673444A2 (en) * 2003-10-16 2006-06-28 The University Court Of The University Of Edinburgh Control of es cell self-renewal and lineage specification, and medium therefor
US20070141703A1 (en) 2003-11-19 2007-06-21 Stanley Edouard G Methods for producing blood products from pluripotent cells in cell culture
US20070269412A1 (en) 2003-12-02 2007-11-22 Celavie Biosciences, Llc Pluripotent cells
US20090155218A1 (en) * 2004-03-10 2009-06-18 Alberto Hayek Compositions and methods for growth of embryonic stem cells
WO2005100551A1 (en) * 2004-04-13 2005-10-27 Sungkwang Educational Foundation In vitro method for isolating, proliferating and differentiating germ-line stem cells
JPWO2005121320A1 (ja) * 2004-06-10 2008-04-10 協和醗酵工業株式会社 幹細胞自己複製促進剤
KR20070083559A (ko) 2004-08-13 2007-08-24 더 유니버시티 오브 죠지아 리서치 파운데이션, 인코포레이티드 인간 배아 줄기세포에서의 자가재생 및 분화를 위한 조성물및 방법
US20080268533A1 (en) 2004-08-25 2008-10-30 University Of Georgia Research Foundation, Inc. Methods and Compositions Utilizing Myc and Gsk3Beta to Manipulate the Pluripotency of Embryonic Stem Cells
CA2591717A1 (en) 2004-12-16 2006-07-06 President And Fellows Of Harvard College Histone demethylation mediated by the nuclear amine oxidase homolog lsd1
US20060182724A1 (en) * 2005-02-15 2006-08-17 Riordan Neil H Method for expansion of stem cells
GB0505970D0 (en) * 2005-03-23 2005-04-27 Univ Edinburgh Culture medium containing kinase inhibitor, and uses thereof
US20060234911A1 (en) * 2005-03-24 2006-10-19 Hoffmann F M Method of reversing epithelial mesenchymal transition
US8999370B2 (en) 2005-05-26 2015-04-07 Thomas Jefferson University Method to treat and prevent posterior capsule opacification
MX2007015610A (es) 2005-06-10 2008-02-21 Irm Llc Compuestos que mantienen la fluripotencia de las celulas totipotentes embrionarias.
WO2007010858A1 (ja) * 2005-07-15 2007-01-25 Kyoto University 骨格筋組織由来の単一細胞よりクローン化した多能性幹細胞
US7601699B2 (en) 2005-08-01 2009-10-13 Nupotential, Inc. Production of reprogrammed cells with restored potential
JP5484728B2 (ja) 2005-08-10 2014-05-07 ジョンズ ホプキンス ユニバーシティ 抗寄生虫治療薬および抗癌治療薬ならびにリジン特異的デメチラーゼ阻害物質として有用なポリアミン類
CA2621161A1 (en) 2005-09-08 2007-03-15 The Government Of The United States Of America As Represented By The Sec Retary Of The Department Of Health And Human Services Methods for promoting stem cell proliferation and survival
KR100679642B1 (ko) * 2005-11-16 2007-02-06 주식회사 알앤엘바이오 인간 지방조직 유래 다분화능 줄기세포 및 이를 함유하는세포치료제
US20090227032A1 (en) 2005-12-13 2009-09-10 Kyoto University Nuclear reprogramming factor and induced pluripotent stem cells
AU2006325975B2 (en) 2005-12-13 2011-12-08 Kyoto University Nuclear reprogramming factor
DE102006001640A1 (de) 2006-01-11 2007-07-12 Degussa Gmbh Keramische Wandverkleidungsverbände mit IR-Strahlung reflektierenden Eigenschaften
JPWO2007088651A1 (ja) 2006-02-01 2009-06-25 国立大学法人 東京大学 ＴＧＦ−βシグナル阻害剤と抗腫瘍剤の組み合せ使用
US7541186B2 (en) 2006-02-22 2009-06-02 University Of Washington Method of generating human retinal progenitors from embryonic stem cells
US9074180B2 (en) 2006-03-30 2015-07-07 The University Court Of The University Of Edinburgh Culture medium containing kinase inhibitors, and uses thereof
GB0615327D0 (en) 2006-03-30 2006-09-13 Univ Edinburgh Culture medium containing kinase inhibitors and uses thereof
US8741643B2 (en) 2006-04-28 2014-06-03 Lifescan, Inc. Differentiation of pluripotent stem cells to definitive endoderm lineage
GB2452186B (en) * 2006-05-02 2011-01-26 Wisconsin Alumni Res Found Method of differentiating stem cells into cells of the endoderm and pancreatic lineage
JP2009542666A (ja) 2006-06-30 2009-12-03 シェーリング コーポレイション Ｐ５３活性を増加させる置換ピペリジンおよびその使用
GB0613756D0 (en) * 2006-07-12 2006-08-23 Univ Sheffield Cell culture medium
EP2044056B1 (en) 2006-07-14 2012-08-22 Novartis AG Pyrimidine derivatives as alk-5 inhibitors
US20080125342A1 (en) 2006-11-07 2008-05-29 Advanced Technology Materials, Inc. Formulations for cleaning memory device structures
GB0622395D0 (en) 2006-11-09 2006-12-20 Univ Cambridge Tech Methods relating to pluripotent cells
WO2008075741A1 (ja) * 2006-12-20 2008-06-26 Keio University 糖尿病治療剤及び予防剤
WO2008089351A1 (en) 2007-01-17 2008-07-24 Wisconsin Alumni Research Foundation Improved culture of stem cells
US9133435B2 (en) * 2007-01-18 2015-09-15 Riken Method for induction/differentiation into photoreceptor cell
US20100172883A1 (en) 2007-01-19 2010-07-08 Bruneau Benoit Gaetan Methods of generating cardiomyocytes
CN103627671A (zh) 2007-01-30 2014-03-12 佐治亚大学研究基金会 产生中内胚层细胞及多能游走细胞的方法与细胞群及用途
US8158415B2 (en) 2007-02-27 2012-04-17 Procell Therapeutics Inc. Combined use of cell permeable Nanog and Oct4 for increasing self-renewal and suppressing differentiation of stem cells
JP2010523685A (ja) 2007-04-13 2010-07-15 ザ・ジョンズ・ホプキンス・ユニバーシティー リジン特異的デメチラーゼ阻害剤
US8648069B2 (en) 2007-06-08 2014-02-11 Abbvie Inc. 5-substituted indazoles as kinase inhibitors
JP2008307007A (ja) 2007-06-15 2008-12-25 Bayer Schering Pharma Ag 出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞
KR20160005142A (ko) 2007-06-29 2016-01-13 셀룰러 다이내믹스 인터내셔널, 인코포레이티드 배아줄기세포 배양을 위한 자동화 방법 및 장치
WO2009032456A2 (en) 2007-08-01 2009-03-12 Primegen Biotech Llc Non-viral delivery of transcription factors that reprogram human somatic cells into a stem cell-like state
EP3078738B1 (en) 2007-08-31 2020-05-20 Whitehead Institute for Biomedical Research Wnt pathway stimulation in reprogramming somatic cells
CA2660123C (en) 2007-10-31 2017-05-09 Kyoto University Nuclear reprogramming method
JP4604076B2 (ja) 2007-11-07 2010-12-22 国立大学法人静岡大学 燃料電池用電解質膜
EP2227540A4 (en) 2007-11-29 2011-11-02 Children S Hospital Of Orange County DIFFERENTIATION OF HUMAN CELLS
US20110190729A1 (en) 2007-11-30 2011-08-04 Cytomatrix Pty Ltd Methods of inducing pluripotency involving sox2 protein
US20110190730A1 (en) 2007-11-30 2011-08-04 Cytomatrix Pty Ltd Methods of inducing pluripotency involving oct4 protein
WO2009117439A2 (en) * 2008-03-17 2009-09-24 The Scripps Research Institute Combined chemical and genetic approaches for generation of induced pluripotent stem cells
WO2010017562A2 (en) * 2008-08-08 2010-02-11 Mayo Foundation For Medical Education And Research Induced pluripotent stem cells
US8298825B1 (en) 2008-08-25 2012-10-30 The General Hospital Corporation TGF-beta receptor inhibitors to enhance direct reprogramming
US8278105B2 (en) * 2008-09-09 2012-10-02 University Of Southern California Induction, propagation and isolation of liver progenitor cells
SG172160A1 (en) 2008-12-17 2011-07-28 Scripps Research Inst Generation and maintenance of stem cells
US20120122212A1 (en) 2009-03-06 2012-05-17 Marica Grskovic Tgf-beta pathway inhibitors for enhancement of cellular reprogramming of human cells
JP5777113B2 (ja) 2009-05-29 2015-09-09 学校法人慶應義塾 人工多能性幹細胞のクローンの選択方法
KR101774206B1 (ko) 2009-08-07 2017-09-04 고쿠리츠 다이가쿠 호진 교토 다이가쿠 유도된 다능성 줄기 세포의 효율적인 확립 방법
CA3091210C (en) 2009-10-16 2023-04-04 The Scripps Research Institute Induction of pluripotent cells
ES2533581T3 (es) 2010-03-05 2015-04-13 Texas Heart Institute ETS2 y Mesp1 generadores de progenitores cardíacos a partir de fibroblastos
ES2893699T3 (es) 2010-03-31 2022-02-09 Scripps Research Inst Reprogramación de células
TWI552751B (zh) 2011-06-20 2016-10-11 Ｈ 朗德貝克公司 投予４－（（１ｒ，３ｓ）－６－氯－３－苯基－二氫茚－１－基）－１，２，２－三甲基－哌及其鹽用於治療精神分裂症的方法

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