ES2966757T3 - Métodos de reprogramación mejorados y plataformas de cultivo celular - Google Patents

Métodos de reprogramación mejorados y plataformas de cultivo celular

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Publication number
ES2966757T3
ES2966757T3 ES15757753T ES15757753T ES2966757T3 ES 2966757 T3 ES2966757 T3 ES 2966757T3 ES 15757753 T ES15757753 T ES 15757753T ES 15757753 T ES15757753 T ES 15757753T ES 2966757 T3 ES2966757 T3 ES 2966757T3
Authority
ES
Spain
Prior art keywords
cell culture
reprogramming methods
culture platforms
improved reprogramming
improved
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES15757753T
Other languages
English (en)
Inventor
Bahram Valamehr
Peter Flynn
Ramzey Abujarour
Megan Robinson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fate Therapeutics Inc
Original Assignee
Fate Therapeutics Inc
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Publication date
Application filed by Fate Therapeutics Inc filed Critical Fate Therapeutics Inc
Application granted granted Critical
Publication of ES2966757T3 publication Critical patent/ES2966757T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/235—Leukemia inhibitory factor [LIF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases (EC 2.)
    • C12N2501/724—Glycosyltransferases (EC 2.4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases (EC 2.)
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
    • C12N2740/15041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/15043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
ES15757753T 2014-03-04 2015-03-04 Métodos de reprogramación mejorados y plataformas de cultivo celular Active ES2966757T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201461947979P 2014-03-04 2014-03-04
PCT/US2015/018801 WO2015134652A1 (en) 2014-03-04 2015-03-04 Improved reprogramming methods and cell culture platforms

Publications (1)

Publication Number Publication Date
ES2966757T3 true ES2966757T3 (es) 2024-04-24

Family

ID=54055848

Family Applications (1)

Application Number Title Priority Date Filing Date
ES15757753T Active ES2966757T3 (es) 2014-03-04 2015-03-04 Métodos de reprogramación mejorados y plataformas de cultivo celular

Country Status (11)

Country Link
US (2) US11268069B2 (es)
EP (3) EP3604499A1 (es)
JP (6) JP6722108B2 (es)
KR (3) KR102460549B1 (es)
CN (1) CN106414721A (es)
AU (2) AU2015227184B2 (es)
CA (1) CA2941004A1 (es)
ES (1) ES2966757T3 (es)
PT (1) PT3114214T (es)
SG (2) SG11201606934SA (es)
WO (1) WO2015134652A1 (es)

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CN114164167A (zh) 2010-12-22 2022-03-11 菲特治疗公司 用于单细胞分选与增强ipsc重新编程的细胞培养平台
EP3604499A1 (en) * 2014-03-04 2020-02-05 Fate Therapeutics, Inc. Improved reprogramming methods and cell culture platforms
CN114058582A (zh) 2015-01-26 2022-02-18 菲特治疗公司 用于诱导造血细胞分化的方法和组合物
KR20180033537A (ko) 2015-07-21 2018-04-03 더 칠드런스 메디칼 센터 코포레이션 Pd-l1 발현 조혈 줄기 세포 및 용도
CA3001917A1 (en) * 2015-10-16 2017-04-20 Fate Therapeutics, Inc. Platform for the induction & maintenance of ground state pluripotency
EP3365428A4 (en) * 2015-10-21 2019-03-20 Indiana University Research&Technology Corporation METHODS FOR PRODUCING SENSORY EPITHELIAL CELLS AND SENSORY NEURONS OF THE INTERNAL EAR IN MAN
KR20180066263A (ko) 2015-11-04 2018-06-18 페이트 세러퓨틱스, 인코포레이티드 조혈 세포 분화를 유도하기 위한 방법 및 조성물
EP3371314B1 (en) 2015-11-04 2023-07-05 Fate Therapeutics, Inc. Genomic engineering of pluripotent cells
CN108778299A (zh) * 2016-01-12 2018-11-09 隆萨沃克斯维尔股份有限公司 生成无载体诱导多能干细胞的载体和方法
CA3048379A1 (en) * 2016-12-27 2018-07-05 Sumitomo Chemical Company, Limited Evaluation method and selection method for induced pluripotent stem cells, and production method for induced pluripotent stem cells
CN106754729B (zh) * 2016-12-31 2020-03-17 内蒙古赛科星家畜种业与繁育生物技术研究院有限公司 利用Xist Tale抑制性转录因子制备诱导性多能干细胞的方法
WO2019060708A1 (en) 2017-09-22 2019-03-28 The Children's Medical Center Corporation TREATMENT OF TYPE 1 DIABETES AND AUTOIMMUNE DISEASES OR DISORDERS
MX2020003739A (es) * 2017-10-11 2020-11-06 Fate Therapeutics Inc Reprogramacion celular mediante el uso de un sistema de expresion de vector plasmidico temporal y transitorio.
EP4083192A1 (en) 2017-12-22 2022-11-02 Fate Therapeutics, Inc. Enhanced immune effector cells and use thereof
CN111511902A (zh) * 2017-12-28 2020-08-07 J·大卫格莱斯顿研究所-根据J·大卫格莱斯顿遗嘱的遗嘱信托 通过crispr激活生成诱导性多能细胞
US20210010030A1 (en) * 2018-03-22 2021-01-14 Nserm (Institut National De La Santé Et De La Recherche Médicale) Method for reprogramming somatic cells
CN109679918A (zh) * 2018-12-25 2019-04-26 合肥中科干细胞再生医学有限公司 一种便捷的人诱导性多能干细胞的制备方法
EP3950932A4 (en) * 2019-03-29 2022-05-25 FUJIFILM Corporation METHOD OF GENERATING PLURIPOTENT STEM CELLS WITH THE ABILITY TO DIFFERENTIATE INTO SPECIFIC CELLS AND THEIR USE
CN110564673A (zh) * 2019-09-26 2019-12-13 广东工业大学 一种干细胞培养液及其应用
CA3150477A1 (en) * 2019-12-11 2021-06-17 Luis Haupt MULTIPLICATION OF STEM CELLS CULTURED IN SUSPENSION IN A BIOREACTOR
US20210395698A1 (en) * 2020-06-22 2021-12-23 Life Technologies Corporation Methods and compositions for cultivating pluripotent cell suspensions
IL301733A (en) * 2020-10-02 2023-05-01 Fate Therapeutics Inc Improving reprogramming and preservation of induced pluripotent stem cells
US20230302044A1 (en) * 2022-03-28 2023-09-28 Steve Reilly Composition to increase cellularlongevity

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