ATE452972T1 - Induzierbares expressionssystem in eukaryotischen zellen - Google Patents

Induzierbares expressionssystem in eukaryotischen zellen

Info

Publication number
ATE452972T1
ATE452972T1 AT02727088T AT02727088T ATE452972T1 AT E452972 T1 ATE452972 T1 AT E452972T1 AT 02727088 T AT02727088 T AT 02727088T AT 02727088 T AT02727088 T AT 02727088T AT E452972 T1 ATE452972 T1 AT E452972T1
Authority
AT
Austria
Prior art keywords
expression system
eukaryotic cells
switch
inducible expression
gene
Prior art date
Application number
AT02727088T
Other languages
English (en)
Inventor
Bernard Massie
Alaka Mullick
Peter Lau
Yasuo Konishi
Original Assignee
Ca Nat Research Council
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ca Nat Research Council filed Critical Ca Nat Research Council
Application granted granted Critical
Publication of ATE452972T1 publication Critical patent/ATE452972T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C07K14/4705—Regulators; Modulating activity stimulating, promoting or activating activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/71—Fusion polypeptide containing domain for protein-protein interaction containing domain for transcriptional activaation, e.g. VP16
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
    • C12N2799/022—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from an adenovirus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/005—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination repressible enhancer/promoter combination, e.g. KRAB
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/15—Vector systems having a special element relevant for transcription chimeric enhancer/promoter combination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT02727088T 2001-05-01 2002-05-01 Induzierbares expressionssystem in eukaryotischen zellen ATE452972T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US28741801P 2001-05-01 2001-05-01
PCT/CA2002/000654 WO2002088346A2 (en) 2001-05-01 2002-05-01 A system for inducible expression in eukaryotic cells

Publications (1)

Publication Number Publication Date
ATE452972T1 true ATE452972T1 (de) 2010-01-15

Family

ID=23102808

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02727088T ATE452972T1 (de) 2001-05-01 2002-05-01 Induzierbares expressionssystem in eukaryotischen zellen

Country Status (7)

Country Link
US (2) US7745592B2 (de)
EP (1) EP1385946B1 (de)
AT (1) ATE452972T1 (de)
AU (1) AU2002257420A1 (de)
CA (1) CA2446110C (de)
DE (1) DE60234824D1 (de)
WO (1) WO2002088346A2 (de)

Families Citing this family (120)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2580515C (en) * 2004-10-04 2017-05-02 National Research Council Of Canada Expression system, components thereof and methods of use
US7935788B2 (en) * 2004-10-04 2011-05-03 National Research Council Of Canada Reverse cumate repressor mutant
WO2007022623A1 (en) * 2005-08-23 2007-03-01 National Research Council Of Canada Regulation of heterologous recombinant protein expression in methylotrophic and methanotrophic bacteria
US20080044855A1 (en) * 2006-03-22 2008-02-21 Yan Xu System for regulated and enhanced baculovirus mediated transient transgene expression in mammalian cells
EP2254998B1 (de) 2008-02-28 2015-12-23 Argos Therapeutics, Inc. Transiente expression immunmodulatorischer polypeptide zur vorbeugung und behandlung von autoimmunkrankheit, allergie und transplantatabstossung
KR102482184B1 (ko) 2010-12-22 2022-12-28 페이트 세러퓨틱스, 인코포레이티드 단세포 분류 및 iPSC의 증강된 재프로그래밍을 위한 세포 배양 플랫폼
WO2012104843A1 (en) 2011-02-06 2012-08-09 Yeda Research And Development Co.Ltd. At The Weizmann Institute Of Science Affinity maturated t cell receptors and use thereof
TR201819015T4 (tr) 2011-06-10 2019-01-21 Bluebird Bio Inc Adrenolökodi̇strofi̇ ve adrenomi̇yelonöropati̇ i̇çi̇n gen terapi̇ vektörleri̇
EP3269802B1 (de) 2011-09-30 2019-10-23 Bluebird Bio, Inc. Verbindungen zur verbesserten viralen transduktion
KR20230065381A (ko) 2012-07-25 2023-05-11 더 브로드 인스티튜트, 인코퍼레이티드 유도 ｄｎａ 결합 단백질 및 게놈 교란 도구 및 이의 적용
JP6486272B2 (ja) 2012-09-07 2019-03-20 マサチューセッツ・アイ・アンド・イア・インファーマリー 有毛細胞および／または支持細胞再生のための方法および組成物
US9950053B2 (en) 2012-10-22 2018-04-24 The Board Of Regents For Oklahoma State University Use of the Salmonella SPP type III secretion proteins as a protective vaccination
US10548962B2 (en) 2012-10-22 2020-02-04 The Board Of Regents For Oklahoma State University Use of the salmonella SPP type III secretion proteins as a protective vaccination
AU2013204922B2 (en) 2012-12-20 2015-05-14 Celgene Corporation Chimeric antigen receptors
JP6493692B2 (ja) 2013-03-15 2019-04-10 セルジーン コーポレイション 修飾されたｔリンパ球
WO2014194130A1 (en) * 2013-05-29 2014-12-04 Joule Unlimited Technologies, Inc. Methods and compositions for controlling gene expression in photosynthetic organisms
US11268069B2 (en) 2014-03-04 2022-03-08 Fate Therapeutics, Inc. Reprogramming methods and cell culture platforms
EP3117004A4 (de) 2014-03-14 2017-12-06 University of Washington Genomische isolatorelemente und verwendungen davon
PL3689899T3 (pl) 2014-04-25 2022-01-31 2Seventy Bio, Inc. Chimeryczne receptory antygenowe promotora mnd
CA2946552A1 (en) 2014-04-25 2015-10-29 Bluebird Bio, Inc. Improved methods for manufacturing adoptive cell therapies
US10023879B2 (en) 2014-06-04 2018-07-17 Fate Therapeutics, Inc. Minimal volume reprogramming of mononuclear cells
DK3151672T3 (da) 2014-06-06 2021-02-01 Bluebird Bio Inc Forbedrede t-celle-sammensætninger
CA2952032C (en) 2014-06-20 2023-03-14 Aveo Pharmaceuticals, Inc. Treatment of chronic kidney disease and other renal dysfunction using a gdf15 modulator
US20170137505A1 (en) 2014-06-20 2017-05-18 Aveo Pharmaceuticals, Inc. Treatment of congestive heart failure and other cardiac dysfunction using a gdf15 modulator
MX2017001079A (es) 2014-07-24 2017-09-12 Bluebird Bio Inc Receptores de antígeno quiméricos de antígeno de maduración de células b (bcma).
PL3197493T3 (pl) 2014-09-25 2021-08-23 Aveo Pharmaceuticals Inc. Sposoby odwracania kacheksji i przedłużania przeżycia obejmujące podanie modulatora gdf15 i środka przeciwnowotworowego
EP3212773B1 (de) 2014-10-29 2021-09-15 Massachusetts Eye and Ear Infirmary Effiziente abgabe von therapeutischen molekülen an zellen des innenohres
JP6325751B2 (ja) 2014-11-04 2018-05-16 ヤンセン ファッシンズ アンド プリベンション ベーフェーＪａｎｓｓｅｎ Ｖａｃｃｉｎｅｓ ＆ Ｐｒｅｖｅｎｔｉｏｎ Ｂ．Ｖ． 治療用ｈｐｖ１６ワクチン
SMT201900596T1 (it) 2014-12-12 2019-11-13 Bluebird Bio Inc Recettori chimerici di antigene per bcma
AU2016309743B2 (en) 2015-08-20 2019-02-07 Janssen Vaccines & Prevention B.V. Therapeutic HPV18 vaccines
EP3344295B1 (de) 2015-08-31 2022-02-23 Helixmith Co., Ltd. Chimäre anti-sialyl-tn-antigenrezeptoren
CN108291237B (zh) 2015-10-16 2023-11-21 菲特治疗公司 用于诱导和维护基态多能性的平台
ES2992295T3 (es) 2016-02-12 2024-12-11 Bluebird Bio Inc Composiciones potenciadoras de Vcn y métodos de uso de las mismas
US11326183B2 (en) 2016-02-12 2022-05-10 Bluebird Bio, Inc. VCN enhancer compositions and methods of using the same
CA3020984A1 (en) * 2016-04-12 2017-10-19 Synploid Biotek, Llc Methods for creating synthetic chromosomes having gene regulatory systems and uses thereof
CN109311963A (zh) 2016-04-14 2019-02-05 蓝鸟生物公司 救助嵌合抗原受体系统
US10517944B2 (en) 2016-05-02 2019-12-31 Janssen Vaccines & Prevention B.V. Therapeutic HPV vaccine combinations
US20170319669A1 (en) 2016-05-03 2017-11-09 Wayne State University Method of enhancing viral-mediated gene delivery
EP3458485B1 (de) 2016-05-19 2021-12-29 The General Hospital Corporation An den rezeptor il-2rbeta gebundenes interleukin-2, eine plattform zur erhöhung der aktivität von natürlichen killer- und regulatorischen t-zellen
EP3500696A4 (de) 2016-08-16 2020-04-08 Bluebird Bio, Inc. Il-10-rezeptor-alpha-homing-endonuklease-varianten, zusammensetzungen und verfahren zur verwendung
MA46059A (fr) 2016-08-23 2019-07-03 Bluebird Bio Inc Variants de l'endonucléase homing tim3, compositions et procédés d'utilisation
ES2952525T3 (es) 2016-09-08 2023-11-02 2Seventy Bio Inc Variantes de endonucleasa homing PD-1, composiciones y métodos de uso
US11332713B2 (en) 2016-11-16 2022-05-17 KSQ Therapeutics, Inc. Gene-regulating compositions and methods for improved immunotherapy
IL309526B2 (en) 2016-11-17 2025-02-01 2Seventy Bio Inc TGFBeta signal converter
ES2969213T3 (es) 2017-02-15 2024-05-17 2Seventy Bio Inc Plantillas de reparación de donantes para edición multiplex del genoma
EP3615664A4 (de) 2017-04-24 2021-01-27 Seattle Children's Hospital (DBA Seattle Children's Research Institute) Homologiegerichtete reparaturzusammensetzungen zur behandlung von hämoglobinopathien
CN111246865A (zh) 2017-07-12 2020-06-05 同生运营公司 程序化以在肿瘤细胞中产生免疫调节剂和抗癌治疗剂的微生物
CN117737009A (zh) * 2017-10-10 2024-03-22 南特生物科学公司 对病毒生产有效载荷具有低毒性的经修饰的ec7细胞
US20190330591A1 (en) 2018-01-10 2019-10-31 Rubius Therapeutics, Inc. Amplifiable rnas for therapeutic cell systems
GB201800903D0 (en) 2018-01-19 2018-03-07 Oxford Genetics Ltd Vectors
WO2019144186A1 (en) 2018-01-23 2019-08-01 Southern Eye Equipment Expression vector and method
AU2019236204B2 (en) 2018-03-15 2026-02-26 KSQ Therapeutics, Inc. Gene-regulating compositions and methods for improved immunotherapy
JP2021518160A (ja) 2018-03-15 2021-08-02 ケーエスキュー セラピューティクス， インコーポレイテッド 免疫療法の改善のための遺伝子調節組成物及び遺伝子調節方法
WO2019217913A1 (en) 2018-05-10 2019-11-14 Sensei Biotherapeutics, Inc. Aspartate beta-hydroxylase chimeric antigen receptors and uses thereof
MX2020012295A (es) 2018-05-15 2021-03-31 Flagship Pioneering Innovations V Inc Composiciones de fusosoma y usos de estas.
US12060419B2 (en) 2018-06-14 2024-08-13 Regeneron Pharmaceuticals, Inc. CD79A chimeric antigen receptors
WO2020006409A1 (en) 2018-06-28 2020-01-02 Trustees Of Boston University Systems and methods for control of gene expression
EP3837275A4 (de) 2018-06-29 2022-07-13 Fred Hutchinson Cancer Center Zellgespeicherte barcodierte tiefe mutationsscannende bibliotheken und deren verwendungen
KR20210043574A (ko) 2018-07-09 2021-04-21 플래그쉽 파이어니어링 이노베이션스 브이, 인크. 푸소좀 조성물 및 이의 용도
EA202190243A1 (ru) 2018-07-11 2021-06-16 Селджин Корпорейшн Применение химерных антигенных рецепторов к bcma
AU2019378881A1 (en) 2018-11-14 2021-06-03 Flagship Pioneering Innovations V, Inc. Fusosome compositions for CNS delivery
US20230043255A1 (en) 2018-11-14 2023-02-09 Flagship Pioneering Innovations V, Inc. Fusosome compositions for t cell delivery
EP3880832A1 (de) 2018-11-14 2021-09-22 Flagship Pioneering Innovations V, Inc. Fusosomzusammensetzungen zur verabreichung von hämatopoetischen stammzellen
CN113366479A (zh) 2018-12-14 2021-09-07 蓝鸟生物公司 二聚化剂调节的免疫受体复合物
GB201901571D0 (en) 2019-02-05 2019-03-27 Oxford Genetics Ltd Inducible AAV sysyem
US12291562B2 (en) 2019-04-01 2025-05-06 Rush University Medical Center Reagents and assays using modified integrin domains
US12109235B2 (en) 2019-06-21 2024-10-08 Kite Pharma, Inc. TGF-beta receptors and methods of use
EP4003508A1 (de) 2019-07-31 2022-06-01 Memorial Sloan Kettering Cancer Center Perfusionsmodulierte tumordosismodellierung mit einzeldosis-strahlentherapie
US20210128619A1 (en) 2019-11-05 2021-05-06 Celgene Corporation Uses of anti-bcma chimeric antigen receptors
GB202004371D0 (en) 2020-03-26 2020-05-13 Glaxosmithkline Ip Dev Ltd CAR constructs
CN113584083A (zh) 2020-04-30 2021-11-02 深圳市深研生物科技有限公司 用于逆转录病毒载体的生产细胞和包装细胞及其制备方法
WO2021236908A2 (en) 2020-05-20 2021-11-25 Biomarin Pharmaceutical Inc. Use of regulatory proteins for the production of adeno-associated virus
CN113699147B (zh) 2020-05-22 2023-06-09 深圳市深研生物科技有限公司 基于四环素和Cumate的共调控序列
WO2021239307A1 (en) 2020-05-27 2021-12-02 Universität Zürich Novel transduction enhancers and uses thereof
US11396646B2 (en) 2020-05-29 2022-07-26 QTG Development, Inc. Steviol glycosyltransferases and genes encoding the same
IL276599A (en) 2020-08-09 2022-03-01 Yeda Res & Dev T cell receptor unique to mage-a1 and its uses
US12152251B2 (en) 2020-08-25 2024-11-26 Kite Pharma, Inc. T cells with improved functionality
US11401531B2 (en) 2020-09-24 2022-08-02 University Of Massachusetts AAV vectors encoding NF1 and uses thereof
EP4241278A2 (de) 2020-11-04 2023-09-13 Celgene Corporation Car-t-zelltherapie bei patienten mit vorheriger antikrebs-alkylatortherapie
JP2023552773A (ja) 2020-12-04 2023-12-19 セルジーン コーポレーション 炎症関連可溶性因子の阻害剤と組み合わせたキメラ抗原受容体（ｃａｒ）ｔ細胞療法の使用
US11821014B2 (en) 2020-12-15 2023-11-21 Frito-Lay North America, Inc. Production of gentisic acid 5-O-β-D xylopyranoside
CN118638226A (zh) 2021-03-19 2024-09-13 葛兰素史密斯克莱知识产权发展有限公司 靶向密蛋白3的嵌合抗原受体和治疗癌症的方法
US11566262B2 (en) 2021-03-24 2023-01-31 Dna Twopointo Inc. Tetracycline-inducible expression systems
CA3214280A1 (en) 2021-04-16 2022-10-20 Julie Ann RYTLEWSKI T cell therapy in patients who have had prior stem cell transplant
KR20240034234A (ko) 2021-07-14 2024-03-13 2세븐티 바이오, 인코포레이티드 항체로부터의 결합 도메인에 융합된 조작된 t 세포 수용체
US20240409906A1 (en) 2021-10-14 2024-12-12 Asimov Inc. Integrases, landing pad architectures, and engineered cells comprising the same
EP4419679A1 (de) 2021-10-20 2024-08-28 University of Rochester Verfahren und zusammensetzungen zur verjüngung von gliapopulationen des zentralnervensystems durch unterdrückung von transkriptionsfaktoren
WO2023069881A1 (en) 2021-10-20 2023-04-27 University Of Rochester Treatment with genetically modified cells, and genetically modified cells per se, with increased competitive advantage and/or decreased competitive disadvantage
KR20240101620A9 (ko) 2021-11-09 2025-12-10 아시모브 인코포레이티드 Aav 벡터 생산을 위한 안정한 생산 시스템
WO2023150557A1 (en) 2022-02-01 2023-08-10 University Of Rochester Methods of generating a population of neurons from human glial progenitor cells and genetic constructs for carrying out such methods
CN119546327A (zh) 2022-04-08 2025-02-28 再生元制药公司 多部分受体和信号传导复合物
KR20250008774A (ko) 2022-05-11 2025-01-15 셀진 코포레이션 T 세포 요법 및 그의 생산과 관련된 방법 및 용도
US20250345432A1 (en) 2022-05-25 2025-11-13 Celgene Corporation Method for predicting response to a t cell therapy
CN119585438A (zh) 2022-05-26 2025-03-07 再生元制药公司 用于维持慢病毒载体的组合物及其用途
JP2025519479A (ja) 2022-06-09 2025-06-26 チョー プラス インコーポレイティド 遺伝子治療ベクター及びウイルスワクチンの高効率で産生するための宿主細胞としての細胞ハイブリッド
WO2025128096A1 (en) 2023-12-13 2025-06-19 CHO Plus Inc. High production of recombinant protein by making cell hybrids and enriching for a preferred mitochondrial phenotype
CN120322549A (zh) 2022-08-25 2025-07-15 生命编辑治疗股份有限公司 利用锁核酸对向导rna化学修饰以用于rna引导的核酸酶介导的基因编辑
TW202417631A (zh) 2022-09-22 2024-05-01 美商拜奧馬林製藥公司 用ａａｖ基因治療載體治療心肌病
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AU2023363959A1 (en) 2022-10-19 2025-03-20 University Of Rochester Methods and compositions for rejuvenating cns glial populations with bcl11a transcription factor expression
EP4634383A1 (de) 2022-12-16 2025-10-22 Life Edit Therapeutics, Inc. Auf das foxp3-gen abzielende guide-rnas und verfahren zur verwendung
EP4634384A1 (de) 2022-12-16 2025-10-22 Life Edit Therapeutics, Inc. Gegen das trac-gen gerichtete guide-rnas und verfahren zur verwendung
CN121152801A (zh) 2023-03-16 2025-12-16 英脉生物医药(杭州)有限公司 靶向ilt7的抗体和其用途
AU2024220143A1 (en) 2023-04-12 2024-10-31 Life Edit Therapeutics, Inc. Compositions and methods for the treatment of huntington's disease by editing the mutant huntingtin gene
KR20260018078A (ko) 2023-05-25 2026-02-06 디스패치 바이오테라퓨틱스, 인크. 암을 치료하기 위한 표적으로서의 합성 암 항원
CN121712794A (zh) 2023-06-29 2026-03-20 迪斯派奇生物治疗公司 合成细胞因子受体
CN121532514A (zh) 2023-07-07 2026-02-13 上海环码生物医药有限公司 用于季节性流感的环状rna疫苗及使用方法
WO2025045142A1 (en) 2023-08-29 2025-03-06 Shanghai Circode Biomed Co., Ltd. Circular rna encoding vegf polypeptides, formulations, and methods of uses
WO2025083619A1 (en) 2023-10-18 2025-04-24 Life Edit Therapeutics, Inc. Rna-guided nucleases and acive fragments and variants thereof and methods of use
WO2025153530A1 (en) 2024-01-16 2025-07-24 Novo Nordisk A/S Albumin-targeted endonucleases, compositions, and methods of use
WO2025155923A1 (en) 2024-01-17 2025-07-24 The Broad Institute, Inc. Aav capsid modifications that enable improved cns-wide gene delivery through interactions with the transferrin receptor
WO2025160155A1 (en) 2024-01-22 2025-07-31 The Broad Institute, Inc. Epigenetic targeting of prion diseases
WO2025171388A1 (en) 2024-02-09 2025-08-14 Dispatch Biotherapeutics, Inc. Engineered cancer antigens with modified domains and related methods and uses
WO2025171383A2 (en) 2024-02-09 2025-08-14 Dispatch Biotherapeutics, Inc. Engineered cancer antigens and related methods and uses
WO2025174908A1 (en) 2024-02-12 2025-08-21 Life Edit Therapeutics, Inc. Novel rna-guided nucleases and proteins for polymerase editing
WO2025217174A1 (en) 2024-04-08 2025-10-16 The Broad Institute, Inc. Aav capsid modifications that enable improved cns-wide gene delivery through interactions with carbonic anhydrase iv
WO2025217163A2 (en) 2024-04-08 2025-10-16 The Broad Institute, Inc. Novel aav capsids binding to human cd59
WO2025214477A1 (en) 2024-04-12 2025-10-16 Skyline Therapeutics (Shanghai) Co., Ltd. Treatment of genetic cardiomyopathies with aav gene therapy vectors
WO2026003754A1 (en) 2024-06-25 2026-01-02 Life Edit Therapeutics, Inc. Novel reverse transcriptases and uses thereof
WO2026006767A1 (en) 2024-06-28 2026-01-02 Dispatch Biotherapeutics, Inc. Tethered il-9/il-9r and related engineered cells and methods

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0303925B1 (de) * 1987-08-17 1995-06-07 F. Hoffmann-La Roche Ag Hochreprimierbare Expressionskontrollsequenzen
EP0705334A1 (de) * 1993-06-14 1996-04-10 Basf Aktiengesellschaft Strenge kontrolle der genexpression in eukaryotischen zellen durch auf tetrazyklin ansprechende promotoren
US5464758A (en) * 1993-06-14 1995-11-07 Gossen; Manfred Tight control of gene expression in eucaryotic cells by tetracycline-responsive promoters
US5866755A (en) 1993-06-14 1999-02-02 Basf Aktiengellschaft Animals transgenic for a tetracycline-regulated transcriptional inhibitor
US5589362A (en) * 1993-06-14 1996-12-31 Basf Aktiengesellschaft Tetracycline regulated transcriptional modulators with altered DNA binding specificities
CA2177085C (en) * 1996-04-26 2007-08-14 National Research Council Of Canada Adenovirus e1-complementing cell lines
US5972650A (en) * 1997-06-26 1999-10-26 Brigham And Women's Hospital Tetracycline repressor regulated mammalian cell transcription and viral replication switch

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US8163894B2 (en) 2012-04-24
US20100210830A1 (en) 2010-08-19
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WO2002088346A3 (en) 2003-10-23
WO2002088346A2 (en) 2002-11-07
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US20040205834A1 (en) 2004-10-14
EP1385946A2 (de) 2004-02-04

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