ATE275628T1 - Expressionssteigernde sequenzelemente (ease) für eukaryontische expressionssysteme - Google Patents

Expressionssteigernde sequenzelemente (ease) für eukaryontische expressionssysteme

Info

Publication number
ATE275628T1
ATE275628T1 AT97902909T AT97902909T ATE275628T1 AT E275628 T1 ATE275628 T1 AT E275628T1 AT 97902909 T AT97902909 T AT 97902909T AT 97902909 T AT97902909 T AT 97902909T AT E275628 T1 ATE275628 T1 AT E275628T1
Authority
AT
Austria
Prior art keywords
expression
ease
eukaryontic
sequence elements
increase sequence
Prior art date
Application number
AT97902909T
Other languages
English (en)
Inventor
Arvia E Morris
Chi-Chang Lee
James N Thomas
Original Assignee
Immunex Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Immunex Corp filed Critical Immunex Corp
Application granted granted Critical
Publication of ATE275628T1 publication Critical patent/ATE275628T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/30—Vector systems having a special element relevant for transcription being an enhancer not forming part of the promoter region
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
AT97902909T 1996-01-11 1997-01-10 Expressionssteigernde sequenzelemente (ease) für eukaryontische expressionssysteme ATE275628T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US58650996A 1996-01-11 1996-01-11
PCT/US1997/000483 WO1997025420A1 (en) 1996-01-11 1997-01-10 Expression augmenting sequence elements (ease) for eukaryotic expression systems

Publications (1)

Publication Number Publication Date
ATE275628T1 true ATE275628T1 (de) 2004-09-15

Family

ID=24346030

Family Applications (1)

Application Number Title Priority Date Filing Date
AT97902909T ATE275628T1 (de) 1996-01-11 1997-01-10 Expressionssteigernde sequenzelemente (ease) für eukaryontische expressionssysteme

Country Status (14)

Country Link
US (3) US6027915A (de)
EP (1) EP0873405B1 (de)
JP (1) JP3495375B2 (de)
KR (1) KR19990077015A (de)
AT (1) ATE275628T1 (de)
AU (1) AU708981B2 (de)
CA (1) CA2241174C (de)
DE (1) DE69730592T2 (de)
DK (1) DK0873405T3 (de)
ES (1) ES2227669T3 (de)
NO (1) NO323277B1 (de)
NZ (1) NZ330926A (de)
PT (1) PT873405E (de)
WO (1) WO1997025420A1 (de)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6596514B2 (en) * 1996-01-11 2003-07-22 Immunex Corporation Expression augmenting sequence elements (EASE) for eukaryotic expression systems
KR19990077015A (ko) * 1996-01-11 1999-10-25 크리스토퍼 엘. 와이트, 스코트 지. 홀퀴스트, 스티븐 엘. 말라스카 진핵 세포 발현 시스템을 위한 발현 증강 서열 요소 (ease)
CA2274987C (en) 1996-12-23 2012-01-24 Immunex Corporation Ligand for receptor activator of nf-kappa b, ligand is member of tnf superfamily
US6521419B1 (en) 1998-09-22 2003-02-18 Kanakaraju Koduri Expression vectors containing hot spot for increased recombinant protein expression in transfected cells
US6800457B2 (en) 1998-09-22 2004-10-05 Bristol-Myers Squibb Company Expression vectors containing hot spot for increased recombinant protein expression in transfected cells
NZ518388A (en) * 1999-10-13 2003-10-31 Immunex Corp Vectors for recombinant protein expression containing polyadenylation sites from SV40 virus and internal ribosome entry sites (IRES).
AU2458001A (en) 1999-12-23 2001-07-03 Zymogenetics Inc. Method for treating inflammation
KR100450266B1 (ko) * 2002-01-16 2004-09-30 주식회사 엘지생명과학 유전자 발현 동물세포 제조용 바이시스트로닉 벡터 및이를 포함하는 세포주 그리고 이를 이용한 항체단백질의생산방법
AU2003228383A1 (en) * 2002-04-09 2003-10-27 Global Biotech Inc. Human tissue urokinase type plasminogen activator production
US20040110295A1 (en) * 2002-05-28 2004-06-10 Maxygen, Inc., A Delaware Corporation Nucleic acid vectors
JP2005031812A (ja) * 2003-07-08 2005-02-03 Toshiba Corp 画像形成装置およびその制御方法
KR100807312B1 (ko) 2005-10-22 2008-02-28 (주)리즈바이오텍 재조합 인간 인슐린 유사 성장 인자 결합 단백질-3의 생산정제 방법
US20080124760A1 (en) 2006-07-26 2008-05-29 Barbara Enenkel Regulatory Nucleic Acid Elements
EP2500416A1 (de) 2006-09-13 2012-09-19 Abbott Laboratories Verbesserungen an Zellkulturen
SG174804A1 (de) 2006-09-13 2011-10-28 Abbott Lab
KR20100014724A (ko) 2007-03-30 2010-02-10 아보트 러보러터리즈 숙주 세포에서 재조합 단백질의 발현을 증강시키기 위한 재조합 발현 벡터 요소(ｒＥＶＥ)
EP2328908A4 (de) 2008-08-28 2012-11-28 Univ New York State Res Found Behandlung von amyloidosen anhand eines myelin-basischen proteins und fragmenten daraus
WO2010023787A1 (ja) * 2008-08-29 2010-03-04 東洋紡績株式会社 遺伝子発現安定化エレメント
CA2744555A1 (en) 2008-11-26 2010-06-03 Five Prime Therapeutics, Inc. Compositions and methods for regulating collagen and smooth muscle actin expression by serpine2
CN102459607B (zh) 2009-06-15 2014-08-06 萨拉基尼克有限公司 新的严格的可选择的标志物
CN103038352B (zh) * 2010-06-15 2015-12-16 萨拉基尼克有限公司 用于增强基因表达的新型基因间元件
KR101599138B1 (ko) 2014-06-17 2016-03-03 한국생명공학연구원 재조합 단백질 발현 증진을 위한 유전자 절편을 포함하는 벡터 및 이의 용도
WO2016003368A1 (en) * 2014-07-01 2016-01-07 Agency For Science, Technology And Research Optimized vectors for the production of recombinant proteins
US20220033845A1 (en) * 2018-09-24 2022-02-03 Merck Sharp & Dohme Corp. Expression vectors for eukaryotic expression systems
KR20210101270A (ko) 2018-12-10 2021-08-18 암젠 인크 돌연변이 피기백 트랜스포사제
EP3898965A4 (de) 2018-12-21 2022-10-12 Merck Sharp & Dohme Corp. Expressionsvektoren für eukaryotische expressionssysteme
JP2022535895A (ja) * 2019-06-07 2022-08-10 バイオコン バイオロジクス リミテッド 哺乳動物発現ベクター
TW202233660A (zh) 2020-10-30 2022-09-01 美商安進公司 過表現胰島素樣生長因子受體突變體以調節ｉｇｆ補充
EP4267717A1 (de) 2020-12-22 2023-11-01 Amgen Inc. Zellkulturverfahren
TW202306975A (zh) 2021-06-10 2023-02-16 美商安進公司 對ｅｒｂｂ４而非ｅｒｂｂ３具有改善的選擇性的工程化ｎｒｇ－１變體
TW202328442A (zh) 2021-09-10 2023-07-16 美商安進公司 平臺宿主對ｉｇｆ—培養基之適應

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3730246A1 (de) * 1987-09-09 1989-06-01 Boehringer Mannheim Gmbh Expressionsvektor und verfahren zur expression von heterologen proteinen in saeugerzellen
KR19990077015A (ko) * 1996-01-11 1999-10-25 크리스토퍼 엘. 와이트, 스코트 지. 홀퀴스트, 스티븐 엘. 말라스카 진핵 세포 발현 시스템을 위한 발현 증강 서열 요소 (ease)

Also Published As

Publication number Publication date
CA2241174A1 (en) 1997-07-17
PT873405E (pt) 2005-01-31
US6312951B1 (en) 2001-11-06
AU1697397A (en) 1997-08-01
JP2000503205A (ja) 2000-03-21
CA2241174C (en) 2006-09-12
NO323277B1 (no) 2007-02-26
WO1997025420A1 (en) 1997-07-17
EP0873405B1 (de) 2004-09-08
ES2227669T3 (es) 2005-04-01
US6027915A (en) 2000-02-22
NZ330926A (en) 1999-11-29
DE69730592D1 (de) 2004-10-14
DK0873405T3 (da) 2005-01-17
DE69730592T2 (de) 2005-09-29
EP0873405A1 (de) 1998-10-28
US6309841B1 (en) 2001-10-30
KR19990077015A (ko) 1999-10-25
EP0873405A4 (de) 2001-10-17
NO983102D0 (no) 1998-07-03
AU708981B2 (en) 1999-08-19
JP3495375B2 (ja) 2004-02-09
NO983102L (no) 1998-09-08

Similar Documents

Publication Publication Date Title
ATE275628T1 (de) Expressionssteigernde sequenzelemente (ease) für eukaryontische expressionssysteme
ES2137931T3 (es) Proteinas gdf-1 y uog-1.
BR0009393A (pt) Polipeptìdeo, sequência de ácido nucleico isolada, construção de ácido nucleico, vetor de expressão recombinante, célula hospedeira recombinante, método para a produção do polipeptìdeo, uso de um polipeptìdeo, e, composição detergente
DE60144145D1 (de) Subtilisin-variante
NZ501767A (en) Cyclic integrin binding peptide with the amino acid sequence RKK
TR199900624T2 (xx) Proteinlerin y�ksek seviyede ekspresyonu.
HUP0001650A2 (hu) Javított expressziós vektorok
ES8602119A1 (es) Metodo para preparar inmunoglobulinas en celulas hospedantes recombinantes
YU8997A (sh) Prečišćen protein koji ima serinsku proteaznu inhibitorsku aktivnost i farmaceutski preparat koji ga sadrži
ZA967173B (en) Purified coprinus laccases and nucleic acids and encoding same
ATE312186T1 (de) Vektoren und verfahren für rekombinante proteinexpression
DK0804558T3 (da) Tripeptidylaminopeptidase
AR014891A1 (es) Genes aislados de proteinas de membranas de mamiferos; reactivos relacionados
PT1025245E (pt) Criacao rapida de linhas celulares estaveis de mamifero produzindo elevados iiveis de proteinas recombinantes
KR960010863A (ko) 재조합 머사시딘 및 이의 제조방법
ES2152313T3 (es) Gen grb-3-3, sus variantes y sus utilizaciones.
NO984491D0 (no) SCF-analogpreparater og fremgangsmÕte for fremstilling av disse
ATE288488T1 (de) Zusammensetzungen und verfahren zur eliminierung unerwünschter zellen
AU2001238064A1 (en) 18477, a human protein kinase and uses therefor
DK1009834T3 (da) Regulering af proteinsekretion
CO5070672A1 (es) Polipeptido de proteina c humana
HK1120077A1 (en) Neutrokine-alpha and neutrokine-alpha splice variant neutrokine-
BR9810046A (pt) Sequência de nucleotìdeos, vetor de expressão, célula hospedeira, proteìna de esperma de mamìfero, proteìna de esperma recombinante, proteìna 4, 1 e/ou 6,7 de esperma, composição farmacêutica, e, vacina contraceptiva

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 0873405

Country of ref document: EP