ATE312186T1 - Vektoren und verfahren für rekombinante proteinexpression - Google Patents

Vektoren und verfahren für rekombinante proteinexpression

Info

Publication number
ATE312186T1
ATE312186T1 AT00970900T AT00970900T ATE312186T1 AT E312186 T1 ATE312186 T1 AT E312186T1 AT 00970900 T AT00970900 T AT 00970900T AT 00970900 T AT00970900 T AT 00970900T AT E312186 T1 ATE312186 T1 AT E312186T1
Authority
AT
Austria
Prior art keywords
polyadenylation signal
methods
expression vector
vectors
protein expression
Prior art date
Application number
AT00970900T
Other languages
English (en)
Inventor
Jeffrey T Mcgrew
Original Assignee
Immunex Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=22571409&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE312186(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Immunex Corp filed Critical Immunex Corp
Application granted granted Critical
Publication of ATE312186T1 publication Critical patent/ATE312186T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/715—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons
    • C07K14/7155—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons for interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
AT00970900T 1999-10-13 2000-10-12 Vektoren und verfahren für rekombinante proteinexpression ATE312186T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US15917799P 1999-10-13 1999-10-13
PCT/US2000/028431 WO2001027299A1 (en) 1999-10-13 2000-10-12 Vectors and methods for recombinant protein expression

Publications (1)

Publication Number Publication Date
ATE312186T1 true ATE312186T1 (de) 2005-12-15

Family

ID=22571409

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00970900T ATE312186T1 (de) 1999-10-13 2000-10-12 Vektoren und verfahren für rekombinante proteinexpression

Country Status (13)

Country Link
US (2) US6632637B1 (de)
EP (1) EP1222298B1 (de)
JP (1) JP4660046B2 (de)
AT (1) ATE312186T1 (de)
AU (1) AU774068B2 (de)
CA (1) CA2386180C (de)
CY (1) CY1105336T1 (de)
DE (1) DE60024659T2 (de)
DK (1) DK1222298T3 (de)
ES (1) ES2252070T3 (de)
IL (2) IL148785A0 (de)
NZ (1) NZ518388A (de)
WO (1) WO2001027299A1 (de)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050005310A1 (en) * 1999-07-12 2005-01-06 Genentech, Inc. Expression vectors and methods
ATE312186T1 (de) * 1999-10-13 2005-12-15 Immunex Corp Vektoren und verfahren für rekombinante proteinexpression
US7691605B2 (en) 2001-09-20 2010-04-06 Immunex Corporation Selection of cells expressing heteromeric polypeptides
US20030157641A1 (en) * 2001-11-16 2003-08-21 Idec Pharmaceuticals Corporation Polycistronic expression of antibodies
ES2557741T3 (es) 2002-03-27 2016-01-28 Immunex Corporation Procedimientos para incrementar la producción de polipéptidos
US20050019925A1 (en) * 2002-11-14 2005-01-27 Genentech, Inc. Intron fusion construct and method of using for selecting high-expressing production cell lines
KR100533794B1 (ko) * 2003-10-02 2005-12-07 주식회사 프로젠 인간 난포자극 호르몬을 대량으로 생산하는 방법
US20080044853A1 (en) * 2004-06-21 2008-02-21 Novozymes A/S Stably Maintained Multiple Copies of at Least Two Orf in the Same Orientation
US20060040354A1 (en) * 2004-07-20 2006-02-23 O'keefe Theresa L Novel polyadenylation signal for use in expression vectors
EP1855111A4 (de) * 2005-03-02 2008-10-01 Astellas Pharma Inc Neuer pd-marker für einen histon-deacetylase-inhibitor
CN101595228A (zh) 2005-07-21 2009-12-02 艾博特公司 包括sorf构建体的多基因表达和使用多蛋白、前体蛋白和蛋白酶解的方法
SG10201510384UA (en) 2006-09-13 2016-01-28 Abbvie Inc Cell culture improvements
EP2500414A1 (de) 2006-09-13 2012-09-19 Abbott Laboratories Verbesserungen an Zellkulturen
US20110014624A1 (en) * 2008-03-12 2011-01-20 Wyeth Llc Methods For Identifying Cells Suitable For Large-Scale Production of Recombinant Proteins
US9309519B2 (en) 2009-03-31 2016-04-12 Celltrion Inc. Method for selecting a high expression recombinant cell line
TW201124535A (en) * 2009-10-30 2011-07-16 Abbott Lab SORF constructs and multiple gene expression
WO2011113841A1 (en) * 2010-03-16 2011-09-22 Robert Steinfeld Eukaryotic vector
EP2598636A4 (de) * 2010-07-26 2014-03-05 Adv Biolog Inc Vektoren und verfahren zur expression rekombinanter proteine
JP5913122B2 (ja) * 2010-11-08 2016-04-27 Ｊｃｒファーマ株式会社 新規発現ベクター
IL210093A0 (en) * 2010-12-19 2011-06-30 David Helman Membrane bound reporter molecules and their use in cell sorting
CA2903226C (en) 2013-03-15 2022-03-15 Bayer Cropscience Lp Constitutive soybean promoters
CA2945325A1 (en) 2014-04-08 2015-10-15 University Of Southern California Polypeptide compositions with type vii collagen fibronectin type iii- like repeats and treatment methods for wound closure and healing
CN107043773B (zh) * 2017-02-08 2018-09-18 四川丰讯科技发展有限公司 一种提高哺乳动物细胞中外源基因表达水平的基因片段、重组载体及其用途
US20210388059A1 (en) 2018-10-29 2021-12-16 Spin Therapeutics, Llc Compositions and methods for alpha-1-antitrypsin disorders
KR20230124578A (ko) 2020-12-22 2023-08-25 암젠 인크 세포 배양 방법

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4965196A (en) * 1983-01-19 1990-10-23 Genentech, Inc. Polycistronic expression vector construction
US4937190A (en) * 1987-10-15 1990-06-26 Wisconsin Alumni Research Foundation Translation enhancer
EP0555203B1 (de) * 1988-12-08 1995-10-18 Abbott Laboratories Verfahren zur induzierbaren genexpression unter verwendung eines dhfr-gens
ATE310817T1 (de) * 1992-09-28 2005-12-15 Chiron Corp Verfahren und zusammensetzungen zur kontrolle der übersetzung der hcv-proteine
US5698446A (en) * 1994-09-07 1997-12-16 Chiron Corporation Methods and compositions for inhibiting production of replication competent virus
DE19514310A1 (de) * 1995-04-18 1996-10-24 Univ Ludwigs Albert Vektoren zur Transfektion von eukaryotischen Zellen, deren Verwendung und damit transfizierte Zielzellen
GB9517263D0 (en) 1995-08-23 1995-10-25 Cancer Res Campaign Tech Expression systems
JP3495375B2 (ja) 1996-01-11 2004-02-09 イミュネックス・コーポレーション 真核生物発現システムの発現増大配列エレメント（ｅａｓｅ）
WO1998017689A2 (en) * 1996-10-18 1998-04-30 Valentis Inc. Il-12 gene expression and delivery systems and uses
ATE312186T1 (de) * 1999-10-13 2005-12-15 Immunex Corp Vektoren und verfahren für rekombinante proteinexpression

Also Published As

Publication number Publication date
US6632637B1 (en) 2003-10-14
CA2386180C (en) 2010-03-09
JP2003511079A (ja) 2003-03-25
IL148785A (en) 2007-09-20
ES2252070T3 (es) 2006-05-16
AU8021600A (en) 2001-04-23
EP1222298B1 (de) 2005-12-07
IL148785A0 (en) 2002-09-12
EP1222298A1 (de) 2002-07-17
CA2386180A1 (en) 2001-04-19
DE60024659T2 (de) 2006-08-17
WO2001027299A1 (en) 2001-04-19
DE60024659D1 (de) 2006-01-12
CY1105336T1 (el) 2010-03-03
US20040063186A1 (en) 2004-04-01
NZ518388A (en) 2003-10-31
DK1222298T3 (da) 2006-04-10
JP4660046B2 (ja) 2011-03-30
AU774068B2 (en) 2004-06-17

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