ATE312186T1 - Vektoren und verfahren für rekombinante proteinexpression - Google Patents
Vektoren und verfahren für rekombinante proteinexpressionInfo
- Publication number
- ATE312186T1 ATE312186T1 AT00970900T AT00970900T ATE312186T1 AT E312186 T1 ATE312186 T1 AT E312186T1 AT 00970900 T AT00970900 T AT 00970900T AT 00970900 T AT00970900 T AT 00970900T AT E312186 T1 ATE312186 T1 AT E312186T1
- Authority
- AT
- Austria
- Prior art keywords
- polyadenylation signal
- methods
- expression vector
- vectors
- protein expression
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/715—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons
- C07K14/7155—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons for interleukins [IL]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
- C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
- Immunology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US15917799P | 1999-10-13 | 1999-10-13 | |
PCT/US2000/028431 WO2001027299A1 (en) | 1999-10-13 | 2000-10-12 | Vectors and methods for recombinant protein expression |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE312186T1 true ATE312186T1 (de) | 2005-12-15 |
Family
ID=22571409
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT00970900T ATE312186T1 (de) | 1999-10-13 | 2000-10-12 | Vektoren und verfahren für rekombinante proteinexpression |
Country Status (13)
Country | Link |
---|---|
US (2) | US6632637B1 (de) |
EP (1) | EP1222298B1 (de) |
JP (1) | JP4660046B2 (de) |
AT (1) | ATE312186T1 (de) |
AU (1) | AU774068B2 (de) |
CA (1) | CA2386180C (de) |
CY (1) | CY1105336T1 (de) |
DE (1) | DE60024659T2 (de) |
DK (1) | DK1222298T3 (de) |
ES (1) | ES2252070T3 (de) |
IL (2) | IL148785A0 (de) |
NZ (1) | NZ518388A (de) |
WO (1) | WO2001027299A1 (de) |
Families Citing this family (25)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20050005310A1 (en) * | 1999-07-12 | 2005-01-06 | Genentech, Inc. | Expression vectors and methods |
CA2386180C (en) * | 1999-10-13 | 2010-03-09 | Immunex Corporation | Vectors and methods for recombinant protein expression |
CA2458811C (en) * | 2001-09-20 | 2011-05-10 | Immunex Corporation | Selection of cells expressing heteromeric polypeptides |
US20030157641A1 (en) * | 2001-11-16 | 2003-08-21 | Idec Pharmaceuticals Corporation | Polycistronic expression of antibodies |
EP1497444B1 (de) | 2002-03-27 | 2015-11-04 | Immunex Corporation | Verfahren zur erhöhung der polypeptidproduktion |
EP1567658A2 (de) * | 2002-11-14 | 2005-08-31 | Genentech, Inc. | Intronfusionskonstrukt undverwendungsverfahren zur selektion hochexprimierender produktionszellinien |
KR100533794B1 (ko) * | 2003-10-02 | 2005-12-07 | 주식회사 프로젠 | 인간 난포자극 호르몬을 대량으로 생산하는 방법 |
WO2005123915A1 (en) * | 2004-06-21 | 2005-12-29 | Novozymes A/S | Stably maintained multiple copies of at least two orf in the same orientation |
WO2006012414A2 (en) * | 2004-07-20 | 2006-02-02 | Critical Therapeutics, Inc. | Novel polyadenylation signal for use in expression vectors |
EP1855111A4 (de) * | 2005-03-02 | 2008-10-01 | Astellas Pharma Inc | Neuer pd-marker für einen histon-deacetylase-inhibitor |
RU2478709C2 (ru) | 2005-07-21 | 2013-04-10 | Эбботт Лэборетриз | ЭКСПРЕССИЯ МНОЖЕСТВА ГЕНОВ, ВКЛЮЧАЯ sORF-КОНСТРУКЦИИ, И СПОСОБЫ ЭКСПРЕССИРОВАНИЯ ИММУНОГЛОБУЛИНА |
NZ575328A (en) | 2006-09-13 | 2012-06-29 | Abbott Lab | Cell culture improvements |
EP2500415A1 (de) | 2006-09-13 | 2012-09-19 | Abbott Laboratories | Verbesserungen an Zellkulturen |
US20110014624A1 (en) * | 2008-03-12 | 2011-01-20 | Wyeth Llc | Methods For Identifying Cells Suitable For Large-Scale Production of Recombinant Proteins |
KR101160048B1 (ko) | 2009-03-31 | 2012-06-26 | (주)셀트리온 | 목적 단백질 고발현 세포주의 선별 방법 |
KR20120091251A (ko) * | 2009-10-30 | 2012-08-17 | 아보트 러보러터리즈 | Sorf 작제물 및 다중 유전자 발현 |
WO2011113841A1 (en) * | 2010-03-16 | 2011-09-22 | Robert Steinfeld | Eukaryotic vector |
EP2598636A4 (de) * | 2010-07-26 | 2014-03-05 | Adv Biolog Inc | Vektoren und verfahren zur expression rekombinanter proteine |
WO2012063799A1 (ja) * | 2010-11-08 | 2012-05-18 | 日本ケミカルリサーチ株式会社 | 新規発現ベクター |
IL210093A0 (en) * | 2010-12-19 | 2011-06-30 | David Helman | Membrane bound reporter molecules and their use in cell sorting |
CN105102474B (zh) | 2013-03-15 | 2019-11-12 | 拜耳作物科学有限合伙公司 | 组成型大豆启动子 |
US10227394B2 (en) | 2014-04-08 | 2019-03-12 | University Of Southern California | Polypeptide compositions with type VII collagen fibronectin type III-like repeats and treatment methods for wound closure and healing |
CN107043773B (zh) * | 2017-02-08 | 2018-09-18 | 四川丰讯科技发展有限公司 | 一种提高哺乳动物细胞中外源基因表达水平的基因片段、重组载体及其用途 |
WO2020092448A1 (en) | 2018-10-29 | 2020-05-07 | Spin Therapeutics, Llc | Compositions and methods for alpha-1-antitrypsin disorders |
US20240083972A1 (en) | 2020-12-22 | 2024-03-14 | Amgen Inc. | Cell culture method |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4965196A (en) * | 1983-01-19 | 1990-10-23 | Genentech, Inc. | Polycistronic expression vector construction |
US4937190A (en) * | 1987-10-15 | 1990-06-26 | Wisconsin Alumni Research Foundation | Translation enhancer |
JP3456992B2 (ja) * | 1988-12-08 | 2003-10-14 | デイモン・バイオテック・インコーポレーテッド | 変異型dhfr遺伝子を使用した発現誘導方法 |
EP0662128B1 (de) * | 1992-09-28 | 2005-11-23 | Chiron Corporation | Verfahren und zusammensetzungen zur kontrolle der übersetzung der hcv-proteine |
US5698446A (en) * | 1994-09-07 | 1997-12-16 | Chiron Corporation | Methods and compositions for inhibiting production of replication competent virus |
DE19514310A1 (de) | 1995-04-18 | 1996-10-24 | Univ Ludwigs Albert | Vektoren zur Transfektion von eukaryotischen Zellen, deren Verwendung und damit transfizierte Zielzellen |
GB9517263D0 (en) | 1995-08-23 | 1995-10-25 | Cancer Res Campaign Tech | Expression systems |
DK0873405T3 (da) | 1996-01-11 | 2005-01-17 | Immunex Corp | Ekspressionsforøgende sekvenselementer (EASE) til eukaryote ekspressionssystemer |
CA2268365A1 (en) * | 1996-10-18 | 1998-04-30 | Jeff Nordstrom | Il-12 gene expression and delivery systems and uses |
CA2386180C (en) * | 1999-10-13 | 2010-03-09 | Immunex Corporation | Vectors and methods for recombinant protein expression |
-
2000
- 2000-10-12 CA CA002386180A patent/CA2386180C/en not_active Expired - Fee Related
- 2000-10-12 EP EP00970900A patent/EP1222298B1/de not_active Expired - Lifetime
- 2000-10-12 DK DK00970900T patent/DK1222298T3/da active
- 2000-10-12 WO PCT/US2000/028431 patent/WO2001027299A1/en active IP Right Grant
- 2000-10-12 AU AU80216/00A patent/AU774068B2/en not_active Ceased
- 2000-10-12 US US09/687,050 patent/US6632637B1/en not_active Expired - Lifetime
- 2000-10-12 JP JP2001530502A patent/JP4660046B2/ja not_active Expired - Lifetime
- 2000-10-12 ES ES00970900T patent/ES2252070T3/es not_active Expired - Lifetime
- 2000-10-12 IL IL14878500A patent/IL148785A0/xx active IP Right Grant
- 2000-10-12 DE DE60024659T patent/DE60024659T2/de not_active Expired - Lifetime
- 2000-10-12 NZ NZ518388A patent/NZ518388A/en unknown
- 2000-10-12 AT AT00970900T patent/ATE312186T1/de not_active IP Right Cessation
-
2002
- 2002-03-20 IL IL148785A patent/IL148785A/en not_active IP Right Cessation
-
2003
- 2003-10-10 US US10/683,775 patent/US20040063186A1/en not_active Abandoned
-
2006
- 2006-02-22 CY CY20061100258T patent/CY1105336T1/el unknown
Also Published As
Publication number | Publication date |
---|---|
EP1222298A1 (de) | 2002-07-17 |
DK1222298T3 (da) | 2006-04-10 |
NZ518388A (en) | 2003-10-31 |
CA2386180C (en) | 2010-03-09 |
JP4660046B2 (ja) | 2011-03-30 |
IL148785A (en) | 2007-09-20 |
AU774068B2 (en) | 2004-06-17 |
ES2252070T3 (es) | 2006-05-16 |
AU8021600A (en) | 2001-04-23 |
US6632637B1 (en) | 2003-10-14 |
WO2001027299A1 (en) | 2001-04-19 |
DE60024659D1 (de) | 2006-01-12 |
JP2003511079A (ja) | 2003-03-25 |
CA2386180A1 (en) | 2001-04-19 |
US20040063186A1 (en) | 2004-04-01 |
IL148785A0 (en) | 2002-09-12 |
EP1222298B1 (de) | 2005-12-07 |
CY1105336T1 (el) | 2010-03-03 |
DE60024659T2 (de) | 2006-08-17 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
UEP | Publication of translation of european patent specification |
Ref document number: 1222298 Country of ref document: EP |
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REN | Ceased due to non-payment of the annual fee |