DK1176205T3 - Ekspression af alkalisk phosphatase i gær - Google Patents

Ekspression af alkalisk phosphatase i gær

Info

Publication number
DK1176205T3
DK1176205T3 DK01117822T DK01117822T DK1176205T3 DK 1176205 T3 DK1176205 T3 DK 1176205T3 DK 01117822 T DK01117822 T DK 01117822T DK 01117822 T DK01117822 T DK 01117822T DK 1176205 T3 DK1176205 T3 DK 1176205T3
Authority
DK
Denmark
Prior art keywords
alkaline phosphatase
expression
yeast
new
host strain
Prior art date
Application number
DK01117822T
Other languages
English (en)
Inventor
Rainer Dr Mueller
Johann-Peter Dr Thalhofer
Frank Dr Geipel
Werner Dr Hoelke
Stephan Dr Glaser
Hellmut Eckstein
Thomas Dr Kirschbaum
Bettina Dr Bommarius
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Application granted granted Critical
Publication of DK1176205T3 publication Critical patent/DK1176205T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/03—Phosphoric monoester hydrolases (3.1.3)
    • C12Y301/03001—Alkaline phosphatase (3.1.3.1)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
DK01117822T 2000-07-25 2001-07-21 Ekspression af alkalisk phosphatase i gær DK1176205T3 (da)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE10036491A DE10036491A1 (de) 2000-07-25 2000-07-25 Expression von Alkalischer Phosphatase in Hefe

Publications (1)

Publication Number Publication Date
DK1176205T3 true DK1176205T3 (da) 2007-07-16

Family

ID=7650333

Family Applications (2)

Application Number Title Priority Date Filing Date
DK06021699.1T DK1792982T3 (da) 2000-07-25 2001-07-21 Codon-optimeret phosphatase og ekspression heraf i gær
DK01117822T DK1176205T3 (da) 2000-07-25 2001-07-21 Ekspression af alkalisk phosphatase i gær

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DK06021699.1T DK1792982T3 (da) 2000-07-25 2001-07-21 Codon-optimeret phosphatase og ekspression heraf i gær

Country Status (11)

Country Link
US (1) US6884602B2 (da)
EP (2) EP1792982B1 (da)
JP (1) JP3657895B2 (da)
CN (1) CN1161461C (da)
AT (2) ATE356881T1 (da)
CA (1) CA2352609C (da)
CZ (1) CZ302558B6 (da)
DE (2) DE10036491A1 (da)
DK (2) DK1792982T3 (da)
ES (2) ES2384108T3 (da)
IL (1) IL144467A (da)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10036491A1 (de) * 2000-07-25 2002-02-07 Roche Diagnostics Gmbh Expression von Alkalischer Phosphatase in Hefe
DE10213201A1 (de) 2002-03-25 2003-10-16 Roche Diagnostics Gmbh Erzeugung von schwach aktiven oder inaktiven Mutanten von Alkalischer Phosphatase und deren Expression in Hefe
EP1460425A1 (en) * 2003-03-17 2004-09-22 Boehringer Mannheim Gmbh Deglycosylated enzymes for conjugates
JP2008005734A (ja) * 2006-06-28 2008-01-17 Kikkoman Corp アルカリホスファターゼ
ES2695999T3 (es) * 2007-10-12 2019-01-11 Hoffmann La Roche Expresión de proteínas a partir de múltiples ácidos nucleicos
WO2010099153A2 (en) 2009-02-25 2010-09-02 Merck Sharp & Dohme Corp. Metabolic engineering of a galactose assimilation pathway in the glycoengineered yeast pichia pastoris
RU2535986C2 (ru) * 2009-02-27 2014-12-20 Новартис Аг Система векторов экспрессии, включающая два селективных маркера
EP2764017B1 (en) * 2011-10-05 2018-01-10 Ben Gurion University of The Negev Research and Development Authority Compositions and methods for conferring herbicide resistance
MX355519B (es) 2012-02-16 2018-04-20 Elanco Us Inc Métodos y composiciones para reducir el impacto ambiental de desecho animal.
JP6732368B2 (ja) * 2016-01-12 2020-07-29 東ソー株式会社 アルカリホスファターゼ及びその製造方法
CN105802979B (zh) * 2016-04-01 2019-09-06 郑州科蒂亚生物技术有限公司 一种小牛肠碱性磷酸酶的表达载体及表达方法
RU2750721C2 (ru) 2017-03-10 2021-07-01 Ф. Хоффманн-Ля Рош Аг Способ получения мультиспецифических антител
CA3057089A1 (en) 2017-03-21 2018-09-27 Synthetic Biologics, Inc. Alkaline phosphatase formulations
US11932860B2 (en) 2017-10-03 2024-03-19 Kikkoman Corporation Method for producing alkaline phosphatase, alkaline phosphatase obtained using said method, and vector and transformant for production thereof
AU2019207600A1 (en) 2018-01-09 2020-07-09 Theriva Biologics, Inc. Alkaline phosphatase agents for treatment of neurodevelopmental disorders
WO2019183209A1 (en) 2018-03-20 2019-09-26 Synthetic Biologics, Inc. Alkaline phosphatase agents for treatment of radiation disorders
WO2019183208A1 (en) 2018-03-20 2019-09-26 Synthetic Biologics, Inc. Intestinal alkaline phosphatase formulations
CN113993541A (zh) * 2019-05-06 2022-01-28 合成生物制品有限公司 基于碱性磷酸酶的肿瘤学治疗
CA3146738A1 (en) * 2019-07-18 2021-01-21 Synthetic Biologics, Inc. Intestinal alkaline phosphatase-based treatments of metabolic disorders
JP7510787B2 (ja) 2020-05-28 2024-07-04 シスメックス株式会社 アルカリフォスファターゼ融合抗体及びその製造方法、並びに免疫測定用試薬及び免疫測定方法
CN113564185B (zh) * 2021-09-07 2023-06-06 金标优尼科(无锡)生物科技有限公司 一种碱性磷酸酶基因及其cho稳定细胞株和alp制备方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2200118A (en) * 1987-01-23 1988-07-27 Allelix Inc Synthetic chymosin-and prochymosin-encoding DNA segments
CA2050601C (en) * 1989-05-10 2000-09-19 Stephen J. Hoffman Production in bacteria and yeast of hemoglobin and analogues thereof
CA2017176A1 (en) * 1989-05-22 1990-11-22 Yoshitomi Pharmaceutical Industries Ltd. Albumin gene-containing plasmid, transformant carrying same, production of such transformant and production of albumin
CA2101610A1 (en) * 1992-08-07 1994-02-08 William D. Prevatt Production of bacillus entomotoxins in methylotrophic yeast
JP2001510048A (ja) * 1997-07-21 2001-07-31 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 形質転換された酵母菌株と、モノターミナルおよびジターミナル脂肪族カルボキシレート生成のためのその使用
DE19819962A1 (de) * 1998-05-05 1999-11-11 Roche Diagnostics Gmbh Hochaktive alkalische Phosphatase
EP1194572A2 (en) * 1999-03-22 2002-04-10 Novozymes Biotech, Inc. Promoter sequences derived from fusarium venenatum and uses thereof
AU2001239205A1 (en) * 2000-03-10 2001-09-17 Novozymes A/S Compositions and methods for producing high yields of heterologous polypeptides in a pichia cell
DE10036491A1 (de) * 2000-07-25 2002-02-07 Roche Diagnostics Gmbh Expression von Alkalischer Phosphatase in Hefe

Also Published As

Publication number Publication date
DE10036491A1 (de) 2002-02-07
DK1792982T3 (da) 2012-07-23
CN1334341A (zh) 2002-02-06
JP2002253269A (ja) 2002-09-10
EP1792982B1 (de) 2012-04-11
IL144467A (en) 2010-05-31
DE50112186D1 (de) 2007-04-26
ATE553191T1 (de) 2012-04-15
US6884602B2 (en) 2005-04-26
EP1176205A2 (de) 2002-01-30
EP1792982A2 (de) 2007-06-06
EP1176205A3 (de) 2002-05-22
JP3657895B2 (ja) 2005-06-08
ATE356881T1 (de) 2007-04-15
US20030096341A1 (en) 2003-05-22
CN1161461C (zh) 2004-08-11
EP1792982A3 (de) 2007-06-13
ES2283360T3 (es) 2007-11-01
CZ20012639A3 (cs) 2002-03-13
EP1176205B1 (de) 2007-03-14
CA2352609C (en) 2008-02-12
CA2352609A1 (en) 2002-01-25
CZ302558B6 (cs) 2011-07-13
IL144467A0 (en) 2002-05-23
ES2384108T3 (es) 2012-06-29

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