DK1792982T3 - Codon-optimeret phosphatase og ekspression heraf i gær - Google Patents

Codon-optimeret phosphatase og ekspression heraf i gær

Info

Publication number
DK1792982T3
DK1792982T3 DK06021699.1T DK06021699T DK1792982T3 DK 1792982 T3 DK1792982 T3 DK 1792982T3 DK 06021699 T DK06021699 T DK 06021699T DK 1792982 T3 DK1792982 T3 DK 1792982T3
Authority
DK
Denmark
Prior art keywords
expression
codon
phosphatase
yeast
optimized
Prior art date
Application number
DK06021699.1T
Other languages
English (en)
Inventor
Rainer Mueller
Johann-Peter Thalhofer
Frank Geipel
Werner Hoelke
Stephan Glaser
Hellmut Eckstein
Thomas Kirschbaum
Bettina Bommarius
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Application granted granted Critical
Publication of DK1792982T3 publication Critical patent/DK1792982T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/03—Phosphoric monoester hydrolases (3.1.3)
    • C12Y301/03001—Alkaline phosphatase (3.1.3.1)
DK06021699.1T 2000-07-25 2001-07-21 Codon-optimeret phosphatase og ekspression heraf i gær DK1792982T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10036491A DE10036491A1 (de) 2000-07-25 2000-07-25 Expression von Alkalischer Phosphatase in Hefe
EP01117822A EP1176205B1 (de) 2000-07-25 2001-07-21 Expression von Alkalischer Phosphatase in Hefe

Publications (1)

Publication Number Publication Date
DK1792982T3 true DK1792982T3 (da) 2012-07-23

Family

ID=7650333

Family Applications (2)

Application Number Title Priority Date Filing Date
DK06021699.1T DK1792982T3 (da) 2000-07-25 2001-07-21 Codon-optimeret phosphatase og ekspression heraf i gær
DK01117822T DK1176205T3 (da) 2000-07-25 2001-07-21 Ekspression af alkalisk phosphatase i gær

Family Applications After (1)

Application Number Title Priority Date Filing Date
DK01117822T DK1176205T3 (da) 2000-07-25 2001-07-21 Ekspression af alkalisk phosphatase i gær

Country Status (11)

Country Link
US (1) US6884602B2 (da)
EP (2) EP1792982B1 (da)
JP (1) JP3657895B2 (da)
CN (1) CN1161461C (da)
AT (2) ATE553191T1 (da)
CA (1) CA2352609C (da)
CZ (1) CZ302558B6 (da)
DE (2) DE10036491A1 (da)
DK (2) DK1792982T3 (da)
ES (2) ES2283360T3 (da)
IL (1) IL144467A (da)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10036491A1 (de) * 2000-07-25 2002-02-07 Roche Diagnostics Gmbh Expression von Alkalischer Phosphatase in Hefe
DE10213201A1 (de) * 2002-03-25 2003-10-16 Roche Diagnostics Gmbh Erzeugung von schwach aktiven oder inaktiven Mutanten von Alkalischer Phosphatase und deren Expression in Hefe
EP1460425A1 (en) 2003-03-17 2004-09-22 Boehringer Mannheim Gmbh Deglycosylated enzymes for conjugates
JP2008005734A (ja) * 2006-06-28 2008-01-17 Kikkoman Corp アルカリホスファターゼ
EP2592147A1 (en) * 2007-10-12 2013-05-15 F. Hoffmann-La Roche AG Protein expression from multiple nucleic acids
US20120003695A1 (en) 2009-02-25 2012-01-05 Davidson Robert C Metabolic engineering of a galactose assimilation pathway in the glycoengineered yeast pichia pastoris
WO2010097240A1 (en) * 2009-02-27 2010-09-02 Novartis Ag Expression vector system comprising two selection markers
EP2764017B1 (en) * 2011-10-05 2018-01-10 Ben Gurion University of The Negev Research and Development Authority Compositions and methods for conferring herbicide resistance
CA2864887C (en) 2012-02-16 2017-04-25 Eli Lilly And Company Methods and compositions for reducing the environmental impact of animal waste
JP6732368B2 (ja) * 2016-01-12 2020-07-29 東ソー株式会社 アルカリホスファターゼ及びその製造方法
CN105802979B (zh) * 2016-04-01 2019-09-06 郑州科蒂亚生物技术有限公司 一种小牛肠碱性磷酸酶的表达载体及表达方法
NZ756132A (en) 2017-03-10 2022-02-25 Hoffmann La Roche Method for producing multispecific antibodies
WO2018175413A1 (en) 2017-03-21 2018-09-27 Synthetic Biologics, Inc. Alkaline phosphatase formulations
JPWO2019069977A1 (ja) 2017-10-03 2020-12-24 キッコーマン株式会社 アルカリホスファターゼの製造方法及びそれを用いて得られるアルカリホスファターゼ、並びにその製造のためのベクター及び形質転換体
EP3737750A4 (en) 2018-01-09 2021-11-17 Synthetic Biologics, Inc. ALKALINE PHOSPHATASE AGENTS FOR THE TREATMENT OF NEURODevelopmental Disorders
WO2019183208A1 (en) 2018-03-20 2019-09-26 Synthetic Biologics, Inc. Intestinal alkaline phosphatase formulations
CA3094174A1 (en) 2018-03-20 2019-09-26 Synthetic Biologics, Inc. Alkaline phosphatase agents for treatment of radiation disorders
CA3146738A1 (en) * 2019-07-18 2021-01-21 Synthetic Biologics, Inc. Intestinal alkaline phosphatase-based treatments of metabolic disorders
JP2021185806A (ja) 2020-05-28 2021-12-13 シスメックス株式会社 アルカリフォスファターゼ融合抗体及びその製造方法、並びに免疫測定用試薬及び免疫測定方法
CN113564185B (zh) * 2021-09-07 2023-06-06 金标优尼科(无锡)生物科技有限公司 一种碱性磷酸酶基因及其cho稳定细胞株和alp制备方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2200118A (en) * 1987-01-23 1988-07-27 Allelix Inc Synthetic chymosin-and prochymosin-encoding DNA segments
CA2050601C (en) * 1989-05-10 2000-09-19 Stephen J. Hoffman Production in bacteria and yeast of hemoglobin and analogues thereof
CA2017176A1 (en) * 1989-05-22 1990-11-22 Yoshitomi Pharmaceutical Industries Ltd. Albumin gene-containing plasmid, transformant carrying same, production of such transformant and production of albumin
CA2101610A1 (en) * 1992-08-07 1994-02-08 William D. Prevatt Production of bacillus entomotoxins in methylotrophic yeast
EP1003881A2 (en) * 1997-07-21 2000-05-31 E.I. Du Pont De Nemours & Company Incorporated Transformed yeast strains and their use for the production of monoterminal and diterminal aliphatic carboxylates
DE19819962A1 (de) * 1998-05-05 1999-11-11 Roche Diagnostics Gmbh Hochaktive alkalische Phosphatase
EP2278016B1 (en) * 1999-03-22 2012-09-26 Novozymes Inc. Promoter sequences derived from Fusarium Venenatum and uses thereof
WO2001066693A1 (en) * 2000-03-10 2001-09-13 Novozymes A/S Compositions and methods for producing high yields of heterologous polypeptides in a pichia cell
DE10036491A1 (de) * 2000-07-25 2002-02-07 Roche Diagnostics Gmbh Expression von Alkalischer Phosphatase in Hefe

Also Published As

Publication number Publication date
US20030096341A1 (en) 2003-05-22
ES2283360T3 (es) 2007-11-01
CA2352609A1 (en) 2002-01-25
DE10036491A1 (de) 2002-02-07
CZ302558B6 (cs) 2011-07-13
DK1176205T3 (da) 2007-07-16
CN1161461C (zh) 2004-08-11
ATE553191T1 (de) 2012-04-15
EP1792982A3 (de) 2007-06-13
ES2384108T3 (es) 2012-06-29
JP3657895B2 (ja) 2005-06-08
EP1176205A3 (de) 2002-05-22
CZ20012639A3 (cs) 2002-03-13
EP1176205A2 (de) 2002-01-30
CA2352609C (en) 2008-02-12
DE50112186D1 (de) 2007-04-26
IL144467A0 (en) 2002-05-23
IL144467A (en) 2010-05-31
ATE356881T1 (de) 2007-04-15
US6884602B2 (en) 2005-04-26
EP1792982B1 (de) 2012-04-11
EP1176205B1 (de) 2007-03-14
JP2002253269A (ja) 2002-09-10
CN1334341A (zh) 2002-02-06
EP1792982A2 (de) 2007-06-06

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