BR122015009611B8 - Moléculas de iniciador e sonda de polinucleotídeo para a detecção de eventos cry1f e cry1ac de algodão, método para detectar a presença dos referidos eventos em uma amostra, bem como kit de detecção de dna - Google Patents

Moléculas de iniciador e sonda de polinucleotídeo para a detecção de eventos cry1f e cry1ac de algodão, método para detectar a presença dos referidos eventos em uma amostra, bem como kit de detecção de dna

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BR122015009611B8
BR122015009611B8 BR122015009611A BR122015009611A BR122015009611B8 BR 122015009611 B8 BR122015009611 B8 BR 122015009611B8 BR 122015009611 A BR122015009611 A BR 122015009611A BR 122015009611 A BR122015009611 A BR 122015009611A BR 122015009611 B8 BR122015009611 B8 BR 122015009611B8
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Brazil
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cotton
events
event
cry1ac
cry1f
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BR122015009611A
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English (en)
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BR122015009611B1 (pt
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Song Ping
Tagliani Laura
W Pellow John
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Dow Agrosciences Llc
Corteva Agriscience Llc
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Application filed by Dow Agrosciences Llc, Corteva Agriscience Llc filed Critical Dow Agrosciences Llc
Publication of BR122015009611B1 publication Critical patent/BR122015009611B1/pt
Publication of BR122015009611B8 publication Critical patent/BR122015009611B8/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
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  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
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  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Insects & Arthropods (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

RESUMO Patente de Invenção: "LINHAGENS TRANSGÊNICAS DE ALGODÃO, CRY1F E CRY1AC, E SUA IDENTIFICAÇÃO ESPECÍFICA DE EVENTO". A presente invenção se refere ao cruzamento de plantas e à proteção de plantas contra insetos. Mais especificamente, esta invenção inclui eventos de transformação inusitados de plantas de algodão, que compreendem uma ou mais seqüências de polinucleotídeos, como aqui descritas, inseridas dentro de um sítio ou sítios específicos dentro do genoma de uma célula de algodão. Em modalidades altamente preferidas, as ditas seqüências de polinucleotídeos codificam as proteínas inibitórias de insetos lepidópteros, Cry1F e Cry1Ac "combinadas". Entretanto, a invenção em questão inclui plantas que têm um único evento cry1F ou cry1Ac, como aqui descrito. Adicionalmente, a invenção se refere a plantas de algodão derivadas desse evento de transformação e ensaios para detectar a presença do evento em uma amostra. Mais especificamente, a presente invenção fornece ensaios de DNA e ensaios relacionados para detectar a presença de certos eventos de resistência a insetos em algodão. Os ensaios baseiam-se nas seqüências de DNA de constructos recombinantes inseridos dentro do genoma do algodão e das seqüências genômicas que flanqueiam os sítios de inserção. Estas seqüências são singulares. Baseado nessas seqüências de inserções e limítrofes, foram gerados iniciadores específicos de eventos. A análise por PCR demonstrou que essas linhagens de algodão podem ser identificadas em diferentes genótipos de algodão por análise dos amplicons da PCR gerados com esses conjuntos de iniciadores específicos de eventos. Assim sendo, estes procedimentos e outros procedimentos afins podem ser usados para identificar singularmente essas linhagens de algodão. São fornecidos também kits e condições úteis para conduzir os ensaios. Estes materiais e métodos podem ser usados também para auxiliar programas de enxertia, a fim de desenvolver traços no algodoeiro. 1/1
BR122015009611A 2004-03-26 2004-10-13 Moléculas de iniciador e sonda de polinucleotídeo para a detecção de eventos cry1f e cry1ac de algodão, método para detectar a presença dos referidos eventos em uma amostra, bem como kit de detecção de dna BR122015009611B8 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US55658604P 2004-03-26 2004-03-26
US61385104P 2004-09-27 2004-09-27
US2005103266 2005-11-03

Publications (2)

Publication Number Publication Date
BR122015009611B1 BR122015009611B1 (pt) 2016-09-06
BR122015009611B8 true BR122015009611B8 (pt) 2022-06-28

Family

ID=34959539

Family Applications (2)

Application Number Title Priority Date Filing Date
BR122015009611A BR122015009611B8 (pt) 2004-03-26 2004-10-13 Moléculas de iniciador e sonda de polinucleotídeo para a detecção de eventos cry1f e cry1ac de algodão, método para detectar a presença dos referidos eventos em uma amostra, bem como kit de detecção de dna
BRPI0418683A BRPI0418683B8 (pt) 2004-03-26 2004-10-13 Polinucleotìdeos referentes aos eventos cry1f 281-24-236 e cry1ac 3006-210-23

Family Applications After (1)

Application Number Title Priority Date Filing Date
BRPI0418683A BRPI0418683B8 (pt) 2004-03-26 2004-10-13 Polinucleotìdeos referentes aos eventos cry1f 281-24-236 e cry1ac 3006-210-23

Country Status (9)

Country Link
US (3) US7179965B2 (pt)
EP (3) EP1737964A1 (pt)
CN (1) CN101027396B (pt)
AR (1) AR046599A1 (pt)
AU (1) AU2004318788B2 (pt)
BR (2) BR122015009611B8 (pt)
ES (2) ES2743789T3 (pt)
HU (1) HUE047016T2 (pt)
WO (1) WO2005103266A1 (pt)

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