AR119062A2 - Evento de maíz mon 87411 - Google Patents

Evento de maíz mon 87411

Info

Publication number
AR119062A2
AR119062A2 ARP200101546A ARP200101546A AR119062A2 AR 119062 A2 AR119062 A2 AR 119062A2 AR P200101546 A ARP200101546 A AR P200101546A AR P200101546 A ARP200101546 A AR P200101546A AR 119062 A2 AR119062 A2 AR 119062A2
Authority
AR
Argentina
Prior art keywords
dna
mon
event
seq
maize
Prior art date
Application number
ARP200101546A
Other languages
English (en)
Inventor
Wen C Burns
Catherine A Chay
Cheryl L Cloninger
Mingqi Deng
Stanislaw Flasinski
Kunsheng Wu
Original Assignee
Monsanto Technology Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Monsanto Technology Llc filed Critical Monsanto Technology Llc
Publication of AR119062A2 publication Critical patent/AR119062A2/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Abstract

La presente proporciona el evento de maíz MON 87411 y plantas, células de plantas, semillas, partes de planta y productos finales que comprenden el evento MON 87411. La misma también proporciona polinucleótidos específicos para el evento MON 87411 y plantas, células de plantas, semillas, partes de planta y productos finales que comprenden polinucleótidos específicos para el evento MON 87411. La presente también proporciona métodos relacionados con el evento MON 87411. Reivindicación 1: Una molécula de ADN recombinante característica del evento de maíz MON 87411, caracterizada porque comprende una secuencia de nucleótidos de SEQ ID Nº 1 o un complemento completo de la misma, donde las secuencias de unión de dicho evento están representadas por las SEQ ID Nº 2 y SEQ ID Nº 3. Reivindicación 4: Una célula vegetal de maíz transgénico, incapaz de regenerar una planta completa caracterizada porque comprende la molécula de ADN de cualquiera de las reivindicaciones 1 - 3. Reivindicación 5: Un método para detectar la presencia de la molécula de ADN de las reivindicaciones 1 - 2 en una muestra biológica, caracterizado porque el método comprende: (a) poner en contacto dicha muestra biológica con una sonda de ADN, donde dicha sonda de ADN comprende SEQ ID Nº 21, SEQ ID Nº 25, o un complemento completo de la misma; (b) someter dicha muestra biológica y la sonda de ADN a condiciones estrictas de hibridación; y (c) detectar la hibridación de dicha sonda de ADN con dicha muestra biológica, en donde la detección de la hibridación es indicativa de la presencia de dicha molécula de ADN en dicha muestra biológica. Reivindicación 11: Un kit de detección de ADN para detectar la molécula de ADN recombinante de las reivindicaciones 1 - 2, caracterizada porque comprende: al menos una molécula de ADN para funcionar como cebador o sonda específica para la molécula de ADN de la reivindicación 1, en donde dicha al menos una molécula de ADN comprende SEQ ID Nº 21, SEQ ID Nº 25 o un complemento completo de la misma. Reivindicación 13: Un método para determinación de cigosidad del ADN de una planta de maíz que comprende la molécula de ADN de la reivindicación 1 característica del evento MON 87411 en una muestra biológica caracterizado porque comprende: (a) poner en contacto dicha muestra con un juego de cebadores que comprende SEQ ID Nº 18 y SEQ ID Nº 24, que (1) cuando se utiliza en una reacción de amplificación de ácido nucleico que comprende el ADN del evento de maíz MON 87411, produce un primer amplicón que es diagnóstico para el evento de maíz MON 87411, y (2) cuando se utiliza en una reacción de amplificación de ácido nucleico que comprende ADN genómico de maíz distinto del ADN de MON 87411, produce un segundo amplicón que es diagnóstico para el ADN genómico de maíz distinto del ADN de MON 87411; (b) realizar una reacción de amplificación de ácido nucleico; y (c) detectar los amplicones así producidos, en donde la detección de la presencia de ambos amplicones indica que dicha muestra es heterocigota para el ADN del evento de maíz MON 87411, en donde la detección de sólo el primer amplicón indica que dicha muestra es homocigota para el ADN del evento de maíz MON 87411.
ARP200101546A 2012-05-08 2020-06-01 Evento de maíz mon 87411 AR119062A2 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201261644368P 2012-05-08 2012-05-08

Publications (1)

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AR119062A2 true AR119062A2 (es) 2021-11-17

Family

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Family Applications (2)

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ARP130101584A AR090981A1 (es) 2012-05-08 2013-05-08 Evento de maiz mon 87411
ARP200101546A AR119062A2 (es) 2012-05-08 2020-06-01 Evento de maíz mon 87411

Family Applications Before (1)

Application Number Title Priority Date Filing Date
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Country Status (17)

Country Link
US (6) US9441240B2 (es)
EP (2) EP3278661B1 (es)
JP (1) JP6315481B2 (es)
CN (1) CN104427861B (es)
AR (2) AR090981A1 (es)
BR (1) BR112014027503A2 (es)
CA (1) CA2872183C (es)
CL (1) CL2014003010A1 (es)
CO (1) CO7151535A2 (es)
CR (1) CR20140557A (es)
ES (2) ES2746748T3 (es)
MX (2) MX371413B (es)
RU (1) RU2648155C2 (es)
UA (2) UA126903C2 (es)
UY (1) UY34799A (es)
WO (1) WO2013169923A2 (es)
ZA (1) ZA201407692B (es)

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