AR127650A2 - Ácido nucleico aislado, método para determinar la cigosidad del adn de una planta de maíz que comprende el evento de maíz dp-004114-3, par de cebadores de polinucleótidos, kit y método para detectar la presencia de una molécula de ácido nucleico que es exclusiva del evento dp-004114-3 - Google Patents
Ácido nucleico aislado, método para determinar la cigosidad del adn de una planta de maíz que comprende el evento de maíz dp-004114-3, par de cebadores de polinucleótidos, kit y método para detectar la presencia de una molécula de ácido nucleico que es exclusiva del evento dp-004114-3Info
- Publication number
- AR127650A2 AR127650A2 ARP220103108A ARP220103108A AR127650A2 AR 127650 A2 AR127650 A2 AR 127650A2 AR P220103108 A ARP220103108 A AR P220103108A AR P220103108 A ARP220103108 A AR P220103108A AR 127650 A2 AR127650 A2 AR 127650A2
- Authority
- AR
- Argentina
- Prior art keywords
- nucleic acid
- dna
- event
- primers
- pair
- Prior art date
Links
- 108020004707 nucleic acids Proteins 0.000 title abstract 14
- 102000039446 nucleic acids Human genes 0.000 title abstract 14
- 150000007523 nucleic acids Chemical class 0.000 title abstract 14
- 238000000034 method Methods 0.000 title abstract 4
- 241001057636 Dracaena deremensis Species 0.000 title abstract 2
- 108020004414 DNA Proteins 0.000 abstract 9
- 240000008042 Zea mays Species 0.000 abstract 8
- 235000002017 Zea mays subsp mays Nutrition 0.000 abstract 8
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 abstract 6
- 235000005822 corn Nutrition 0.000 abstract 6
- 230000003321 amplification Effects 0.000 abstract 3
- 238000001514 detection method Methods 0.000 abstract 3
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 3
- 108091033319 polynucleotide Proteins 0.000 abstract 3
- 102000040430 polynucleotide Human genes 0.000 abstract 3
- 239000002157 polynucleotide Substances 0.000 abstract 3
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 abstract 2
- 235000009973 maize Nutrition 0.000 abstract 2
- 108091093088 Amplicon Proteins 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Plant Pathology (AREA)
- Immunology (AREA)
- Botany (AREA)
- Pest Control & Pesticides (AREA)
- Insects & Arthropods (AREA)
- Mycology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physiology (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Crystallography & Structural Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
Abstract
Ácido nucleico aislado que consiste de SEQ ID Nº 32, y complementos de longitud completa de la misma, diagnóstico de la presencia de semilla del evento de maíz DP-004114-3 que fue depositada en American Type Culture Collection (ATCC) con número de acceso PTA-11506. Método para determinar la cigosidad del ADN de una planta de maíz que comprende el dicho evento de maíz DP-004114-3 que comprende: (a) poner en contacto dicha muestra con el par de cebadores de SEQ ID Nº 29 y 30, de modo que (1) cuando se utilice en una reacción de amplificación de ácido nucleico que comprende el ADN del evento de maíz DP-004114-3, produzca un primer ácido nucleico de la reivindicación 1 que es un diagnóstico del evento de maíz DP-004114-3, y (2) cuando se utilice en una reacción de amplificación de ácido nucleico que comprende un ADN genómico de maíz distinto del ADN de DP-004114-3, produzca un segundo ácido nucleico aislado que es diagnóstico de ADN genómico de maíz distinto del ADN de DP-004114-3, (b) llevar a cabo una reacción de amplificación de ácido nucleico y (c) detectar los ácidos nucleicos así producidos, donde la detección de la presencia de ambos ácidos nucleicos indica que dicha muestra es heterocigota para el ADN del evento de maíz DP-004114-3, en donde la detección de solamente el primer ácido nucleico indica que dicha muestra es homocigota para el ADN del evento de maíz DP-004114-3. Par de cebadores de polinucleótidos, en donde ambos el primer cebador de polinucleótidos y el segundo cebador de polinucleótido contienen etiquetas detectables, y que funcionan juntos en presencia de una plantilla de ADN de DP-004114-3 en una muestra para producir el amplicón de la SEQ ID Nº 32 de diagnóstico para el evento DP-004114-3. Kit para detectar ácidos nucleicos que son únicos para el evento DP-004114-3 que comprende un par de cebadores compuestos por SEQ ID Nº 29 y 30, o SEQ ID Nº 33 y 34, y complementos de las mismas, en donde los pares de cebadores contienen etiquetas detectables, que funcionan como un par de cebadores en un método de detección de ácidos nucleicos. También se revela un método para detectar la presencia de una molécula de ácido nucleico que es exclusiva del evento DP-004114-3 que utiliza el par de cebadores compuesto por SEQ ID Nº 29 y 30.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261617990P | 2012-04-24 | 2012-04-24 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR127650A2 true AR127650A2 (es) | 2024-02-14 |
Family
ID=48083682
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP130101044A AR090558A1 (es) | 2012-04-24 | 2013-03-27 | Evento de maiz dp-004114-3 y metodos para su deteccion |
| ARP220103108A AR127650A2 (es) | 2012-04-24 | 2022-11-11 | Ácido nucleico aislado, método para determinar la cigosidad del adn de una planta de maíz que comprende el evento de maíz dp-004114-3, par de cebadores de polinucleótidos, kit y método para detectar la presencia de una molécula de ácido nucleico que es exclusiva del evento dp-004114-3 |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP130101044A AR090558A1 (es) | 2012-04-24 | 2013-03-27 | Evento de maiz dp-004114-3 y metodos para su deteccion |
Country Status (14)
| Country | Link |
|---|---|
| US (4) | US9725772B2 (es) |
| EP (1) | EP2831243B1 (es) |
| CN (2) | CN104411828A (es) |
| AR (2) | AR090558A1 (es) |
| BR (1) | BR112014026728B1 (es) |
| CA (1) | CA2871557C (es) |
| EA (1) | EA034330B1 (es) |
| ES (1) | ES2816640T3 (es) |
| HU (1) | HUE050690T2 (es) |
| MX (1) | MX2014012894A (es) |
| PL (1) | PL2831243T3 (es) |
| UA (1) | UA116982C2 (es) |
| WO (1) | WO2013149018A1 (es) |
| ZA (1) | ZA201407686B (es) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PL2512226T3 (pl) * | 2009-12-17 | 2019-10-31 | Pioneer Hi Bred Int | Modyfikacja DP-004114-3 kukurydzy i sposoby jej wykrywania |
| AR090558A1 (es) * | 2012-04-24 | 2014-11-19 | Pioneer Hi Bred Int | Evento de maiz dp-004114-3 y metodos para su deteccion |
| CN105779610B (zh) * | 2015-08-06 | 2019-01-18 | 中国农业科学院生物技术研究所 | 转基因抗虫玉米hgk60外源插入片段的旁侧序列及其检测方法 |
| WO2022026540A1 (en) * | 2020-07-31 | 2022-02-03 | Inari Agriculture Technology, Inc. | Inht26 transgenic soybean |
| CN112831584B (zh) * | 2021-01-27 | 2023-06-06 | 隆平生物技术(海南)有限公司 | 转基因玉米事件lp007-2及其检测方法 |
| CN113354721B (zh) * | 2021-06-18 | 2022-08-05 | 山东省农业科学院 | 一种调控玉米籽粒胚发育的基因Emb1637的应用 |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4945050A (en) | 1984-11-13 | 1990-07-31 | Cornell Research Foundation, Inc. | Method for transporting substances into living cells and tissues and apparatus therefor |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4800159A (en) | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
| US6083499A (en) | 1996-04-19 | 2000-07-04 | Mycogen Corporation | Pesticidal toxins |
| US5981840A (en) | 1997-01-24 | 1999-11-09 | Pioneer Hi-Bred International, Inc. | Methods for agrobacterium-mediated transformation |
| US6340593B1 (en) | 1998-10-23 | 2002-01-22 | Mycogen Corporation | Plant-optimized polynucleotides encoding approximately 15 kDa and approximately 45 kDa pesticidal proteins |
| AR025349A1 (es) | 1999-08-23 | 2002-11-20 | Mycogen Corp | Metodos para controlar las plagas del gusano gris |
| US6395485B1 (en) | 2000-01-11 | 2002-05-28 | Aventis Cropscience N.V. | Methods and kits for identifying elite event GAT-ZM1 in biological samples |
| PT1620571E (pt) | 2003-05-02 | 2015-09-03 | Du Pont | Milho do evento tc1507 e métodos para deteção deste |
| JP2008514233A (ja) | 2004-09-29 | 2008-05-08 | パイオニア ハイ−ブレッド インターナショナル， インコーポレイテッド | トウモロコシイベントｄａｓ−５９１２２−７およびその検出のための方法 |
| WO2006053147A1 (en) | 2004-11-12 | 2006-05-18 | Pioneer Hi-Bred International, Inc. | Method of evaluating plant protection |
| AP3797A (en) * | 2006-05-26 | 2016-08-31 | Monsanto Technology Llc | Corn plant and seed corresponding to transgenic event mon89034 and methods for detection and use thereof |
| KR101405030B1 (ko) * | 2006-06-03 | 2014-06-11 | 신젠타 파티서페이션즈 아게 | 옥수수 이벤트 ｍｉｒ162 |
| CN101641443A (zh) * | 2006-10-30 | 2010-02-03 | 先锋高级育种国际公司 | 玉米事件dp-098140-6，鉴定和/或检测其的组合物和方法 |
| CN101568641A (zh) | 2006-12-22 | 2009-10-28 | 先锋高级育种国际公司 | 转基因作物的抗性治理策略 |
| WO2009088732A1 (en) | 2008-01-10 | 2009-07-16 | Pioneer Hi-Bred International, Inc. | Novel bacillus thuringiensis gene with coleopteran activity |
| WO2009100188A2 (en) | 2008-02-08 | 2009-08-13 | Dow Agrosciences Llc | Methods for detection of corn event das-59132 |
| PL2512226T3 (pl) * | 2009-12-17 | 2019-10-31 | Pioneer Hi Bred Int | Modyfikacja DP-004114-3 kukurydzy i sposoby jej wykrywania |
| US20110154526A1 (en) * | 2009-12-17 | 2011-06-23 | Pioneer Hi-Bred International, Inc. | Maize event DP-043A47-3 and methods for detection thereof |
| AR090558A1 (es) * | 2012-04-24 | 2014-11-19 | Pioneer Hi Bred Int | Evento de maiz dp-004114-3 y metodos para su deteccion |
-
2013
- 2013-03-27 AR ARP130101044A patent/AR090558A1/es active IP Right Grant
- 2013-03-28 BR BR112014026728-6A patent/BR112014026728B1/pt active IP Right Grant
- 2013-03-28 CN CN201380028463.2A patent/CN104411828A/zh active Pending
- 2013-03-28 EP EP13715578.4A patent/EP2831243B1/en active Active
- 2013-03-28 HU HUE13715578A patent/HUE050690T2/hu unknown
- 2013-03-28 EA EA201491810A patent/EA034330B1/ru unknown
- 2013-03-28 UA UAA201411674A patent/UA116982C2/uk unknown
- 2013-03-28 CN CN201811462556.7A patent/CN109468314B/zh active Active
- 2013-03-28 MX MX2014012894A patent/MX2014012894A/es active IP Right Grant
- 2013-03-28 PL PL13715578T patent/PL2831243T3/pl unknown
- 2013-03-28 ES ES13715578T patent/ES2816640T3/es active Active
- 2013-03-28 CA CA2871557A patent/CA2871557C/en active Active
- 2013-03-28 WO PCT/US2013/034374 patent/WO2013149018A1/en active Application Filing
- 2013-03-29 US US13/853,413 patent/US9725772B2/en active Active
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2014
- 2014-10-22 ZA ZA2014/07686A patent/ZA201407686B/en unknown
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2017
- 2017-07-12 US US15/648,112 patent/US10407688B2/en active Active
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2019
- 2019-07-19 US US16/516,341 patent/US20190338300A1/en not_active Abandoned
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2020
- 2020-08-19 US US16/997,530 patent/US20200385749A1/en not_active Abandoned
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2022
- 2022-11-11 AR ARP220103108A patent/AR127650A2/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| US20170369953A1 (en) | 2017-12-28 |
| MX2014012894A (es) | 2015-10-15 |
| CN109468314A (zh) | 2019-03-15 |
| US20130283483A1 (en) | 2013-10-24 |
| US9725772B2 (en) | 2017-08-08 |
| EP2831243B1 (en) | 2020-07-01 |
| EA034330B1 (ru) | 2020-01-29 |
| CA2871557A1 (en) | 2013-10-03 |
| WO2013149018A1 (en) | 2013-10-03 |
| HUE050690T2 (hu) | 2020-12-28 |
| US20200385749A1 (en) | 2020-12-10 |
| PL2831243T3 (pl) | 2021-01-25 |
| US10407688B2 (en) | 2019-09-10 |
| CN109468314B (zh) | 2022-06-24 |
| ZA201407686B (en) | 2015-10-28 |
| UA116982C2 (uk) | 2018-06-11 |
| ES2816640T3 (es) | 2021-04-05 |
| BR112014026728B1 (pt) | 2022-02-22 |
| CA2871557C (en) | 2022-09-13 |
| EP2831243A1 (en) | 2015-02-04 |
| CN104411828A (zh) | 2015-03-11 |
| EA201491810A1 (ru) | 2015-03-31 |
| AR090558A1 (es) | 2014-11-19 |
| BR112014026728A2 (pt) | 2017-07-11 |
| US20190338300A1 (en) | 2019-11-07 |
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