BR112021015080B1 - Micro-organismo produtor de l-tirosina, método para produzir ltirosina com o uso do mesmo, cassete de expressão e composição para produzir l-tirosina - Google Patents
Micro-organismo produtor de l-tirosina, método para produzir ltirosina com o uso do mesmo, cassete de expressão e composição para produzir l-tirosina Download PDFInfo
- Publication number
- BR112021015080B1 BR112021015080B1 BR112021015080-3A BR112021015080A BR112021015080B1 BR 112021015080 B1 BR112021015080 B1 BR 112021015080B1 BR 112021015080 A BR112021015080 A BR 112021015080A BR 112021015080 B1 BR112021015080 B1 BR 112021015080B1
- Authority
- BR
- Brazil
- Prior art keywords
- tyrosine
- gene
- microorganism
- trp
- promoter
- Prior art date
Links
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 title claims abstract description 253
- 244000005700 microbiome Species 0.000 title claims abstract description 86
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 65
- 230000014509 gene expression Effects 0.000 title claims abstract description 21
- 239000000203 mixture Substances 0.000 title claims abstract description 13
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 155
- 230000001105 regulatory effect Effects 0.000 claims abstract description 105
- 108010015724 Prephenate Dehydratase Proteins 0.000 claims abstract description 36
- 229960004441 tyrosine Drugs 0.000 claims description 119
- 101150023849 pheA gene Proteins 0.000 claims description 60
- 238000011144 upstream manufacturing Methods 0.000 claims description 47
- 241000186216 Corynebacterium Species 0.000 claims description 8
- 239000002773 nucleotide Substances 0.000 claims description 8
- 125000003729 nucleotide group Chemical group 0.000 claims description 8
- 108010076504 Protein Sorting Signals Proteins 0.000 claims description 4
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 67
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 60
- 238000000034 method Methods 0.000 description 52
- 239000012634 fragment Substances 0.000 description 43
- 229960004799 tryptophan Drugs 0.000 description 41
- 239000013598 vector Substances 0.000 description 41
- 229960005190 phenylalanine Drugs 0.000 description 31
- 238000006116 polymerization reaction Methods 0.000 description 27
- 210000004027 cell Anatomy 0.000 description 25
- 238000004925 denaturation Methods 0.000 description 25
- 230000036425 denaturation Effects 0.000 description 25
- 241000186226 Corynebacterium glutamicum Species 0.000 description 21
- 108091033319 polynucleotide Proteins 0.000 description 20
- 102000040430 polynucleotide Human genes 0.000 description 20
- 239000002157 polynucleotide Substances 0.000 description 20
- 239000002609 medium Substances 0.000 description 19
- 101150044170 trpE gene Proteins 0.000 description 19
- 101100408135 Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) phnA gene Proteins 0.000 description 18
- 229940024606 amino acid Drugs 0.000 description 18
- 235000001014 amino acid Nutrition 0.000 description 17
- 102000004169 proteins and genes Human genes 0.000 description 17
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 15
- 150000001413 amino acids Chemical class 0.000 description 15
- 101150083154 tyrA gene Proteins 0.000 description 15
- 241000588724 Escherichia coli Species 0.000 description 14
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 13
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 13
- 238000012408 PCR amplification Methods 0.000 description 13
- 238000000137 annealing Methods 0.000 description 13
- 239000013611 chromosomal DNA Substances 0.000 description 13
- 238000010353 genetic engineering Methods 0.000 description 13
- 230000009466 transformation Effects 0.000 description 13
- 101150076125 aroG gene Proteins 0.000 description 11
- 230000002759 chromosomal effect Effects 0.000 description 11
- 239000013612 plasmid Substances 0.000 description 11
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 10
- 101150024271 TKT gene Proteins 0.000 description 10
- 239000003153 chemical reaction reagent Substances 0.000 description 10
- 238000010367 cloning Methods 0.000 description 10
- 238000004520 electroporation Methods 0.000 description 10
- 230000006801 homologous recombination Effects 0.000 description 10
- 238000002744 homologous recombination Methods 0.000 description 10
- 238000011534 incubation Methods 0.000 description 10
- 238000002156 mixing Methods 0.000 description 10
- 235000018102 proteins Nutrition 0.000 description 10
- 108091008146 restriction endonucleases Proteins 0.000 description 10
- 230000037361 pathway Effects 0.000 description 9
- 238000012163 sequencing technique Methods 0.000 description 9
- GPMWEYVOEWNYSK-QMMMGPOBSA-N (2s)-2-amino-n-hydroxy-3-(4-hydroxyphenyl)propanamide Chemical compound ONC(=O)[C@@H](N)CC1=CC=C(O)C=C1 GPMWEYVOEWNYSK-QMMMGPOBSA-N 0.000 description 8
- BHHGXPLMPWCGHP-UHFFFAOYSA-N Phenethylamine Chemical compound NCCC1=CC=CC=C1 BHHGXPLMPWCGHP-UHFFFAOYSA-N 0.000 description 8
- 230000000694 effects Effects 0.000 description 8
- 101150033780 ilvB gene Proteins 0.000 description 8
- 101150087199 leuA gene Proteins 0.000 description 8
- 101150081723 leuC gene Proteins 0.000 description 8
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 7
- 101100123410 Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) hacA gene Proteins 0.000 description 7
- 101100181662 Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) leuC1 gene Proteins 0.000 description 7
- 101150087955 glf gene Proteins 0.000 description 7
- 101150025049 leuB gene Proteins 0.000 description 7
- FPWMCUPFBRFMLH-UHFFFAOYSA-N prephenic acid Chemical compound OC1C=CC(CC(=O)C(O)=O)(C(O)=O)C=C1 FPWMCUPFBRFMLH-UHFFFAOYSA-N 0.000 description 7
- 230000008844 regulatory mechanism Effects 0.000 description 7
- NGHMDNPXVRFFGS-IUYQGCFVSA-N D-erythrose 4-phosphate Chemical compound O=C[C@H](O)[C@H](O)COP(O)(O)=O NGHMDNPXVRFFGS-IUYQGCFVSA-N 0.000 description 6
- 108020004414 DNA Proteins 0.000 description 6
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 6
- 101150010999 aroP gene Proteins 0.000 description 6
- WTFXTQVDAKGDEY-UHFFFAOYSA-N (-)-chorismic acid Natural products OC1C=CC(C(O)=O)=CC1OC(=C)C(O)=O WTFXTQVDAKGDEY-UHFFFAOYSA-N 0.000 description 5
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 5
- 101100002724 Thermus thermophilus aroH gene Proteins 0.000 description 5
- 230000006696 biosynthetic metabolic pathway Effects 0.000 description 5
- 238000012217 deletion Methods 0.000 description 5
- 230000037430 deletion Effects 0.000 description 5
- 229960000310 isoleucine Drugs 0.000 description 5
- 238000013518 transcription Methods 0.000 description 5
- 230000035897 transcription Effects 0.000 description 5
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 241000588722 Escherichia Species 0.000 description 4
- 229930182844 L-isoleucine Natural products 0.000 description 4
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 4
- 241000588902 Zymomonas mobilis Species 0.000 description 4
- -1 aromatic amino acid Chemical class 0.000 description 4
- 125000003118 aryl group Chemical group 0.000 description 4
- 229940041514 candida albicans extract Drugs 0.000 description 4
- WTFXTQVDAKGDEY-HTQZYQBOSA-L chorismate(2-) Chemical compound O[C@@H]1C=CC(C([O-])=O)=C[C@H]1OC(=C)C([O-])=O WTFXTQVDAKGDEY-HTQZYQBOSA-L 0.000 description 4
- 210000000349 chromosome Anatomy 0.000 description 4
- 238000012136 culture method Methods 0.000 description 4
- 230000012010 growth Effects 0.000 description 4
- 238000003780 insertion Methods 0.000 description 4
- 230000037431 insertion Effects 0.000 description 4
- 229960003136 leucine Drugs 0.000 description 4
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 4
- 235000019796 monopotassium phosphate Nutrition 0.000 description 4
- DTBNBXWJWCWCIK-UHFFFAOYSA-K phosphonatoenolpyruvate Chemical compound [O-]C(=O)C(=C)OP([O-])([O-])=O DTBNBXWJWCWCIK-UHFFFAOYSA-K 0.000 description 4
- 239000002243 precursor Substances 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- IFGCUJZIWBUILZ-UHFFFAOYSA-N sodium 2-[[2-[[hydroxy-(3,4,5-trihydroxy-6-methyloxan-2-yl)oxyphosphoryl]amino]-4-methylpentanoyl]amino]-3-(1H-indol-3-yl)propanoic acid Chemical compound [Na+].C=1NC2=CC=CC=C2C=1CC(C(O)=O)NC(=O)C(CC(C)C)NP(O)(=O)OC1OC(C)C(O)C(O)C1O IFGCUJZIWBUILZ-UHFFFAOYSA-N 0.000 description 4
- 235000000346 sugar Nutrition 0.000 description 4
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 4
- 239000012138 yeast extract Substances 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 108020004705 Codon Proteins 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- 235000019454 L-leucine Nutrition 0.000 description 3
- 239000004395 L-leucine Substances 0.000 description 3
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 3
- 238000010276 construction Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 230000009123 feedback regulation Effects 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 238000004128 high performance liquid chromatography Methods 0.000 description 3
- 230000005764 inhibitory process Effects 0.000 description 3
- 239000003550 marker Substances 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 230000035772 mutation Effects 0.000 description 3
- 229930029653 phosphoenolpyruvate Natural products 0.000 description 3
- 229920001184 polypeptide Polymers 0.000 description 3
- 108090000765 processed proteins & peptides Proteins 0.000 description 3
- 102000004196 processed proteins & peptides Human genes 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 239000013587 production medium Substances 0.000 description 3
- 101150109655 ptsG gene Proteins 0.000 description 3
- 230000002829 reductive effect Effects 0.000 description 3
- 238000006467 substitution reaction Methods 0.000 description 3
- 230000002195 synergetic effect Effects 0.000 description 3
- PJWIPEXIFFQAQZ-PUFIMZNGSA-N 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonic acid Chemical compound OP(=O)(O)OC[C@@H](O)[C@@H](O)[C@H](O)CC(=O)C(O)=O PJWIPEXIFFQAQZ-PUFIMZNGSA-N 0.000 description 2
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 2
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 101100364969 Dictyostelium discoideum scai gene Proteins 0.000 description 2
- 239000007836 KH2PO4 Substances 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- 101100261636 Methanothermobacter marburgensis (strain ATCC BAA-927 / DSM 2133 / JCM 14651 / NBRC 100331 / OCM 82 / Marburg) trpB2 gene Proteins 0.000 description 2
- 101100364971 Mus musculus Scai gene Proteins 0.000 description 2
- DFPAKSUCGFBDDF-UHFFFAOYSA-N Nicotinamide Chemical compound NC(=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-UHFFFAOYSA-N 0.000 description 2
- 101150001834 PTS gene Proteins 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- 239000002202 Polyethylene glycol Substances 0.000 description 2
- 101100370749 Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) trpC1 gene Proteins 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 229960002685 biotin Drugs 0.000 description 2
- 235000020958 biotin Nutrition 0.000 description 2
- 239000011616 biotin Substances 0.000 description 2
- 239000006227 byproduct Substances 0.000 description 2
- FAPWYRCQGJNNSJ-UBKPKTQASA-L calcium D-pantothenic acid Chemical compound [Ca+2].OCC(C)(C)[C@@H](O)C(=O)NCCC([O-])=O.OCC(C)(C)[C@@H](O)C(=O)NCCC([O-])=O FAPWYRCQGJNNSJ-UBKPKTQASA-L 0.000 description 2
- 239000001110 calcium chloride Substances 0.000 description 2
- 229910001628 calcium chloride Inorganic materials 0.000 description 2
- 229960002079 calcium pantothenate Drugs 0.000 description 2
- 239000004202 carbamide Substances 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 230000002900 effect on cell Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 239000013604 expression vector Substances 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- IPCSVZSSVZVIGE-UHFFFAOYSA-N hexadecanoic acid Chemical compound CCCCCCCCCCCCCCCC(O)=O IPCSVZSSVZVIGE-UHFFFAOYSA-N 0.000 description 2
- 238000009396 hybridization Methods 0.000 description 2
- 230000008676 import Effects 0.000 description 2
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 description 2
- 229940049920 malate Drugs 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 235000019319 peptone Nutrition 0.000 description 2
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 2
- 108010071189 phosphoenolpyruvate-glucose phosphotransferase Proteins 0.000 description 2
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- 229920001223 polyethylene glycol Polymers 0.000 description 2
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 230000006798 recombination Effects 0.000 description 2
- 238000005215 recombination Methods 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 238000011218 seed culture Methods 0.000 description 2
- 229960003495 thiamine Drugs 0.000 description 2
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 2
- 101150019416 trpA gene Proteins 0.000 description 2
- 101150081616 trpB gene Proteins 0.000 description 2
- 101150111232 trpB-1 gene Proteins 0.000 description 2
- 101150016309 trpC gene Proteins 0.000 description 2
- 101150100816 trpD gene Proteins 0.000 description 2
- 101150079930 trpGD gene Proteins 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 description 1
- QDGAVODICPCDMU-UHFFFAOYSA-N 2-amino-3-[3-[bis(2-chloroethyl)amino]phenyl]propanoic acid Chemical compound OC(=O)C(N)CC1=CC=CC(N(CCCl)CCCl)=C1 QDGAVODICPCDMU-UHFFFAOYSA-N 0.000 description 1
- LXCUAFVVTHZALS-UHFFFAOYSA-N 3-(3-methoxyphenyl)piperidine Chemical compound COC1=CC=CC(C2CNCCC2)=C1 LXCUAFVVTHZALS-UHFFFAOYSA-N 0.000 description 1
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- ATRRKUHOCOJYRX-UHFFFAOYSA-N Ammonium bicarbonate Chemical compound [NH4+].OC([O-])=O ATRRKUHOCOJYRX-UHFFFAOYSA-N 0.000 description 1
- 239000004254 Ammonium phosphate Substances 0.000 description 1
- 101100082080 Bacillus subtilis (strain 168) pabA gene Proteins 0.000 description 1
- 241000186146 Brevibacterium Species 0.000 description 1
- WTFXTQVDAKGDEY-HTQZYQBOSA-N Chorismic acid Natural products O[C@@H]1C=CC(C(O)=O)=C[C@H]1OC(=C)C(O)=O WTFXTQVDAKGDEY-HTQZYQBOSA-N 0.000 description 1
- 241000186145 Corynebacterium ammoniagenes Species 0.000 description 1
- 241001644925 Corynebacterium efficiens Species 0.000 description 1
- 241000186308 Corynebacterium stationis Species 0.000 description 1
- 241000337023 Corynebacterium thermoaminogenes Species 0.000 description 1
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N DMSO Substances CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 1
- 238000007702 DNA assembly Methods 0.000 description 1
- 230000007018 DNA scission Effects 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241000588698 Erwinia Species 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- 108700028146 Genetic Enhancer Elements Proteins 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 150000008553 L-tyrosines Chemical class 0.000 description 1
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
- 102000003960 Ligases Human genes 0.000 description 1
- 108090000364 Ligases Proteins 0.000 description 1
- OYHQOLUKZRVURQ-HZJYTTRNSA-N Linoleic acid Chemical compound CCCCC\C=C/C\C=C/CCCCCCCC(O)=O OYHQOLUKZRVURQ-HZJYTTRNSA-N 0.000 description 1
- WHXSMMKQMYFTQS-UHFFFAOYSA-N Lithium Chemical compound [Li] WHXSMMKQMYFTQS-UHFFFAOYSA-N 0.000 description 1
- 102000004317 Lyases Human genes 0.000 description 1
- 108090000856 Lyases Proteins 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 108010052285 Membrane Proteins Proteins 0.000 description 1
- 102000018697 Membrane Proteins Human genes 0.000 description 1
- VZUNGTLZRAYYDE-UHFFFAOYSA-N N-methyl-N'-nitro-N-nitrosoguanidine Chemical compound O=NN(C)C(=N)N[N+]([O-])=O VZUNGTLZRAYYDE-UHFFFAOYSA-N 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 235000021314 Palmitic acid Nutrition 0.000 description 1
- 235000019483 Peanut oil Nutrition 0.000 description 1
- 108091000080 Phosphotransferase Proteins 0.000 description 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
- 241000588768 Providencia Species 0.000 description 1
- 101100297447 Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) phnB gene Proteins 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 1
- 241000607720 Serratia Species 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- 235000019764 Soybean Meal Nutrition 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 108091081024 Start codon Proteins 0.000 description 1
- 235000021355 Stearic acid Nutrition 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 108091023045 Untranslated Region Proteins 0.000 description 1
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 241000319304 [Brevibacterium] flavum Species 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 238000007792 addition Methods 0.000 description 1
- 235000004279 alanine Nutrition 0.000 description 1
- 125000003295 alanine group Chemical group N[C@@H](C)C(=O)* 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 229930013930 alkaloid Natural products 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 239000001099 ammonium carbonate Substances 0.000 description 1
- 235000012501 ammonium carbonate Nutrition 0.000 description 1
- 235000019270 ammonium chloride Nutrition 0.000 description 1
- 229910000148 ammonium phosphate Inorganic materials 0.000 description 1
- 235000019289 ammonium phosphates Nutrition 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 150000001491 aromatic compounds Chemical class 0.000 description 1
- 230000002238 attenuated effect Effects 0.000 description 1
- 150000007514 bases Chemical class 0.000 description 1
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 235000010216 calcium carbonate Nutrition 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 239000001506 calcium phosphate Substances 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 235000011010 calcium phosphates Nutrition 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 125000002091 cationic group Chemical group 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 235000010980 cellulose Nutrition 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000003240 coconut oil Substances 0.000 description 1
- 235000019864 coconut oil Nutrition 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 239000013601 cosmid vector Substances 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- 229940127089 cytotoxic agent Drugs 0.000 description 1
- 239000002254 cytotoxic agent Substances 0.000 description 1
- 231100000599 cytotoxic agent Toxicity 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- MNNHAPBLZZVQHP-UHFFFAOYSA-N diammonium hydrogen phosphate Chemical compound [NH4+].[NH4+].OP([O-])([O-])=O MNNHAPBLZZVQHP-UHFFFAOYSA-N 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 235000015872 dietary supplement Nutrition 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- 235000019797 dipotassium phosphate Nutrition 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 235000013373 food additive Nutrition 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 238000012224 gene deletion Methods 0.000 description 1
- 238000012239 gene modification Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000005017 genetic modification Effects 0.000 description 1
- 235000013617 genetically modified food Nutrition 0.000 description 1
- 230000034659 glycolysis Effects 0.000 description 1
- 235000013882 gravy Nutrition 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 150000002484 inorganic compounds Chemical class 0.000 description 1
- 229910010272 inorganic material Inorganic materials 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 238000004255 ion exchange chromatography Methods 0.000 description 1
- 229910000358 iron sulfate Inorganic materials 0.000 description 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 1
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 235000020778 linoleic acid Nutrition 0.000 description 1
- OYHQOLUKZRVURQ-IXWMQOLASA-N linoleic acid Natural products CCCCC\C=C/C\C=C\CCCCCCCC(O)=O OYHQOLUKZRVURQ-IXWMQOLASA-N 0.000 description 1
- 239000002502 liposome Substances 0.000 description 1
- 229910052744 lithium Inorganic materials 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 125000001360 methionine group Chemical group N[C@@H](CCSC)C(=O)* 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000000520 microinjection Methods 0.000 description 1
- 235000013379 molasses Nutrition 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- WQEPLUUGTLDZJY-UHFFFAOYSA-N n-Pentadecanoic acid Natural products CCCCCCCCCCCCCCC(O)=O WQEPLUUGTLDZJY-UHFFFAOYSA-N 0.000 description 1
- 229960003966 nicotinamide Drugs 0.000 description 1
- 235000005152 nicotinamide Nutrition 0.000 description 1
- 239000011570 nicotinamide Substances 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 description 1
- OQCDKBAXFALNLD-UHFFFAOYSA-N octadecanoic acid Natural products CCCCCCCC(C)CCCCCCCCC(O)=O OQCDKBAXFALNLD-UHFFFAOYSA-N 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 235000019198 oils Nutrition 0.000 description 1
- 235000014593 oils and fats Nutrition 0.000 description 1
- 239000000312 peanut oil Substances 0.000 description 1
- 230000004108 pentose phosphate pathway Effects 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 235000014786 phosphorus Nutrition 0.000 description 1
- 102000020233 phosphotransferase Human genes 0.000 description 1
- 239000013600 plasmid vector Substances 0.000 description 1
- FPWMCUPFBRFMLH-HDKIZWTHSA-N prephenic acid Chemical compound O[C@H]1C=C[C@](CC(=O)C(O)=O)(C(O)=O)C=C1 FPWMCUPFBRFMLH-HDKIZWTHSA-N 0.000 description 1
- 210000001236 prokaryotic cell Anatomy 0.000 description 1
- 125000001500 prolyl group Chemical group [H]N1C([H])(C(=O)[*])C([H])([H])C([H])([H])C1([H])[H] 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000004455 soybean meal Substances 0.000 description 1
- 239000003549 soybean oil Substances 0.000 description 1
- 235000012424 soybean oil Nutrition 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008117 stearic acid Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 235000020238 sunflower seed Nutrition 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000011426 transformation method Methods 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
- 101150034486 trpG gene Proteins 0.000 description 1
- 101150006320 trpR gene Proteins 0.000 description 1
- 241001515965 unidentified phage Species 0.000 description 1
- 239000004474 valine Substances 0.000 description 1
- 108700026220 vif Genes Proteins 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/22—Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
- C12P13/225—Tyrosine; 3,4-Dihydroxyphenylalanine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
- C12N15/71—Expression systems using regulatory sequences derived from the trp-operon
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/22—Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y402/00—Carbon-oxygen lyases (4.2)
- C12Y402/01—Hydro-lyases (4.2.1)
- C12Y402/01051—Prephenate dehydratase (4.2.1.51)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y504/00—Intramolecular transferases (5.4)
- C12Y504/99—Intramolecular transferases (5.4) transferring other groups (5.4.99)
- C12Y504/99005—Chorismate mutase (5.4.99.5)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
- C12Y103/01—Oxidoreductases acting on the CH-CH group of donors (1.3) with NAD+ or NADP+ as acceptor (1.3.1)
- C12Y103/01013—Prephenate dehydrogenase (NADP+) (1.3.1.13)
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020190071797A KR102134418B1 (ko) | 2019-06-17 | 2019-06-17 | L-타이로신을 생산하는 미생물 및 이를 이용한 l-타이로신 생산 방법 |
KR10-2019-0071797 | 2019-06-17 | ||
PCT/KR2020/007858 WO2020256415A1 (ko) | 2019-06-17 | 2020-06-17 | L-타이로신을 생산하는 미생물 및 이를 이용한 l-타이로신 생산 방법 |
Publications (2)
Publication Number | Publication Date |
---|---|
BR112021015080A2 BR112021015080A2 (US06244707-20010612-C00010.png) | 2021-12-28 |
BR112021015080B1 true BR112021015080B1 (pt) | 2024-01-09 |
Family
ID=71839750
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112021015080-3A BR112021015080B1 (pt) | 2019-06-17 | 2020-06-17 | Micro-organismo produtor de l-tirosina, método para produzir ltirosina com o uso do mesmo, cassete de expressão e composição para produzir l-tirosina |
Country Status (10)
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
MX2022011083A (es) * | 2021-05-20 | 2023-03-16 | Cj Cheiljedang Corp | Promotor novedoso y uso del mismo. |
KR102685904B1 (ko) | 2021-05-20 | 2024-07-19 | 씨제이제일제당 주식회사 | 신규 프로모터 및 이의 용도 |
Family Cites Families (36)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ZA811368B (en) * | 1980-03-24 | 1982-04-28 | Genentech Inc | Bacterial polypedtide expression employing tryptophan promoter-operator |
JPS619282A (ja) * | 1984-06-22 | 1986-01-16 | Hitachi Ltd | 遺伝子組換え菌の培養方法 |
JP3185261B2 (ja) * | 1991-02-05 | 2001-07-09 | 味の素株式会社 | 発酵法による芳香族アミノ酸の製造法 |
JPH04248983A (ja) * | 1991-02-05 | 1992-09-04 | Ajinomoto Co Inc | 発酵法による芳香族アミノ酸の製造法 |
DE19818541C2 (de) * | 1998-04-24 | 2003-04-10 | Forschungszentrum Juelich Gmbh | Mikrobielle Herstellung von Substanzen aus dem aromatischen Stoffwechsel / III |
KR101158592B1 (ko) * | 2003-10-21 | 2012-06-22 | 카아길, 인코포레이팃드 | 모나틴 및 모나틴 전구체의 생산 |
US7482140B2 (en) * | 2004-06-15 | 2009-01-27 | Ajinomoto Co., Inc. | L-tyrosine-producing bacterium and a method for producing L-tyrosine |
JP4665613B2 (ja) * | 2004-06-15 | 2011-04-06 | 味の素株式会社 | L−チロシン生産菌及びl−チロシンの製造法 |
CN103642882A (zh) * | 2004-06-25 | 2014-03-19 | 协和发酵生化株式会社 | 二肽的制造方法 |
KR100620092B1 (ko) | 2004-12-16 | 2006-09-08 | 씨제이 주식회사 | 코리네박테리움 속 세포로부터 유래된 신규한 프로모터서열, 그를 포함하는 발현 카세트 및 벡터, 상기 벡터를포함하는 숙주 세포 및 그를 이용하여 유전자를 발현하는방법 |
KR100653742B1 (ko) * | 2004-12-30 | 2006-12-05 | 씨제이 주식회사 | 신규한 l-라이신-유도성 프로모터 |
DE102005019040A1 (de) * | 2005-04-23 | 2006-10-26 | Degussa Ag | Verfahren zur Herstellung von L-Aminosäuren unter Verwendung verbesserter Stämme der Familie Enterobacteriaceae |
US7700328B2 (en) | 2006-06-07 | 2010-04-20 | E.I. Du Pont De Nemours And Company | Method for producing an L-tyrosine over-producing bacterial strain |
JP5756259B2 (ja) | 2006-09-15 | 2015-07-29 | シージェイ チェイルジェダン コーポレイション | L−リジン生産能の向上したコリネバクテリウム属およびそれを用いたl−リジン生産方法 |
US20080102499A1 (en) * | 2006-10-27 | 2008-05-01 | Lori Jean Templeton | Method of enhancing L-tyrosine production in recombinant bacteria |
KR100792095B1 (ko) * | 2006-12-29 | 2008-01-04 | 씨제이 주식회사 | L-페닐알라닌 생산능을 갖는 대장균 변이주로부터유전자조작된 l-트립토판 생산능을 갖는 재조합 대장균균주 및 이를 이용한 트립토판 제조방법 |
EP2336347B1 (en) * | 2008-09-08 | 2017-03-15 | Ajinomoto Co., Inc. | An l-amino acid-producing microorganism and a method for producing an l-amino acid |
EP2395096B1 (en) * | 2009-02-09 | 2014-04-09 | Kyowa Hakko Bio Co., Ltd. | Process for producing L-amino acids |
US8283152B2 (en) * | 2009-08-28 | 2012-10-09 | Cj Cheiljedang Corporation | Microorganism producing O-acetyl-homoserine and the method of producing O-acetyl-homoserine using the microorganism |
CN102399835A (zh) * | 2011-10-14 | 2012-04-04 | 江南大学 | 一种微生物发酵生产l-苯丙氨酸的方法 |
KR101327093B1 (ko) * | 2012-01-06 | 2013-11-07 | 씨제이제일제당 (주) | L-아미노산을 생산할 수 있는 미생물 및 이를 이용하여 l-아미노산을 생산하는 방법 |
RU2593957C2 (ru) * | 2012-01-10 | 2016-08-10 | СиДжей ЧЕИЛДЗЕДАНГ КОРПОРЕЙШН | МИКРООРГАНИЗМ ИЗ РОДА Escherichia, ОБЛАДАЮЩИЙ УСИЛЕННОЙ СПОСОБНОСТЬЮ К ПРОДУКЦИИ L-ТРИПТОФАНА, И СПОСОБ ПОЛУЧЕНИЯ L-ТРИПТОФАНА С ЕГО ИСПОЛЬЗОВАНИЕМ |
EP2628792A1 (de) * | 2012-02-17 | 2013-08-21 | Evonik Industries AG | Zelle mit verringerter ppGppase-Aktivität |
CN103074292B (zh) * | 2013-01-22 | 2014-07-09 | 江南大学 | 一种高产l-苯丙氨酸的重组谷氨酸棒杆菌及其应用 |
KR101721722B1 (ko) * | 2013-03-11 | 2017-04-10 | 씨제이제일제당 (주) | L-발린 생산능이 향상된 균주 및 이를 이용한 l-발린 생산방법 |
ES2643442T3 (es) * | 2013-04-16 | 2017-11-22 | Cj Cheiljedang Corporation | Microorganismo que presenta productividad de L-triptófano y método para la producción del L-triptófano mediante la utilización del mismo |
JP3185261U (ja) * | 2013-05-29 | 2013-08-08 | 広太 小西 | 花束保持具 |
DK2811028T3 (en) * | 2013-06-03 | 2017-05-01 | Evonik Degussa Gmbh | Process for Preparation of L-Valine Using Recombinant Coryn Bacteria Containing the Propionate Inducible IlvBN Operon |
KR101518860B1 (ko) * | 2013-10-11 | 2015-05-12 | 씨제이제일제당 (주) | L-아미노산의 생산 방법 |
KR101599802B1 (ko) * | 2014-05-23 | 2016-03-04 | 씨제이제일제당 주식회사 | 세포내 에너지 수준이 향상된 미생물 및 이를 이용하여 l-아미노산을 생산하는 방법 |
KR101835173B1 (ko) * | 2014-06-23 | 2018-03-07 | 씨제이제일제당 (주) | L-트립토판 생산능을 갖는 에스케리키아속 미생물 및 이를 이용한 l-트립토판의 제조 방법 |
KR101835935B1 (ko) * | 2014-10-13 | 2018-03-12 | 씨제이제일제당 (주) | L-아르기닌을 생산하는 코리네박테리움 속 미생물 및 이를 이용한 l-아르기닌의 제조 방법 |
ES2932086T3 (es) * | 2016-12-28 | 2023-01-11 | Cj Cheiljedang Corp | Una variante novedosa de la isopropilmalato sintasa y un método para producir L-leucina mediante el uso de la misma |
CN109536428B (zh) * | 2018-12-07 | 2022-08-30 | 武汉远大弘元股份有限公司 | 一种产l-异亮氨酸的基因工程菌及其构建方法和应用 |
CN109628365A (zh) * | 2019-01-09 | 2019-04-16 | 江南大学 | 一种可提高大肠杆菌酪氨酸产量的方法 |
CN109554325B (zh) * | 2019-01-09 | 2020-11-03 | 江南大学 | 一种可高产酪氨酸的大肠杆菌工程菌及其应用 |
-
2019
- 2019-06-17 KR KR1020190071797A patent/KR102134418B1/ko active IP Right Grant
-
2020
- 2020-06-17 WO PCT/KR2020/007858 patent/WO2020256415A1/ko unknown
- 2020-06-17 AR ARP200101706A patent/AR119176A1/es unknown
- 2020-06-17 EP EP20826047.1A patent/EP3901265A4/en active Pending
- 2020-06-17 JP JP2021547750A patent/JP7267441B2/ja active Active
- 2020-06-17 AU AU2020295264A patent/AU2020295264B2/en active Active
- 2020-06-17 CN CN202080021043.1A patent/CN114072492B/zh active Active
- 2020-06-17 CA CA3127994A patent/CA3127994A1/en active Pending
- 2020-06-17 US US17/430,314 patent/US20220195470A1/en active Pending
- 2020-06-17 BR BR112021015080-3A patent/BR112021015080B1/pt active IP Right Grant
Also Published As
Publication number | Publication date |
---|---|
JP7267441B2 (ja) | 2023-05-01 |
CN114072492A (zh) | 2022-02-18 |
EP3901265A4 (en) | 2022-03-30 |
JP2022521384A (ja) | 2022-04-07 |
BR112021015080A2 (US06244707-20010612-C00010.png) | 2021-12-28 |
AR119176A1 (es) | 2021-12-01 |
US20220195470A1 (en) | 2022-06-23 |
AU2020295264A1 (en) | 2021-08-12 |
CN114072492B (zh) | 2024-04-30 |
WO2020256415A1 (ko) | 2020-12-24 |
EP3901265A1 (en) | 2021-10-27 |
AU2020295264B2 (en) | 2023-05-18 |
KR102134418B1 (ko) | 2020-07-16 |
CA3127994A1 (en) | 2020-12-24 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP6998466B2 (ja) | クエン酸シンターゼの活性が弱化された変異型ポリペプチド及びそれを用いたl-アミノ酸生産方法 | |
RU2761871C1 (ru) | Новый экспортер L-триптофана и способ продуцирования L-триптофана с его использованием | |
JP7295276B2 (ja) | L-トレオニン排出タンパク質の変異体及びそれを用いたl-トレオニン生産方法 | |
BR112019018036B1 (pt) | Variante de aspartoquinase inovadora e método para produzir laminoácido com o uso da mesma | |
BR112021000765B1 (pt) | Variante de proteína de exportação de l-triptofano e seu uso, polinucleotídeo que codifica a dita variante, vetor, microrganismo que produz l-triptofano e método para produzir l- triptofano | |
JP7394868B2 (ja) | L-ヒスチジン生産能が強化された微生物及びそれを用いたヒスチジン生産方法 | |
BR112015018599B1 (pt) | Micro-organismos com produtividade de putrescina e processo para produzir putrescina que utiliza os mesmos | |
BR112013014441B1 (pt) | Microrganismo tendo uma melhor capacidade de produzir ornitina e método para produzir ornitina usando o mesmo | |
BR112017028181B1 (pt) | Micro-organismo modificado para produzir putrescina ou ornitina e método para produzir putrescina ou ornitina usando o dito micro-organismo | |
BR112021015080B1 (pt) | Micro-organismo produtor de l-tirosina, método para produzir ltirosina com o uso do mesmo, cassete de expressão e composição para produzir l-tirosina | |
BR112016023337B1 (pt) | Microrganismo tendo capacidade de produção de l-lisina e método de produção de l-lisina utilizando o mesmo | |
KR20200123905A (ko) | L-쓰레오닌 생산능이 강화된 미생물 및 이를 이용한 쓰레오닌 생산방법 | |
BR112019019867B1 (pt) | Micro-organismo para produzir l-aminoácido com atividade aprimorada de a-glucosidase e método para produzir l-aminoácido com o uso do mesmo | |
JP7547511B2 (ja) | 新規l-チロシン排出タンパク質変異体及びそれを用いてl-チロシンを生産する方法 | |
AU2022235362A1 (en) | Microorganism Of The Genus Corynebacterium For Producing L-Amino Acid And Method For Producing L-Amino Acid Using The Same | |
RU2779461C1 (ru) | Микроорганизмы для получения l-тирозина и способ получения l-тирозина с их использованием | |
JP2023506053A (ja) | クエン酸シンターゼの活性が弱化した新規な変異型ポリペプチド及びそれを用いたl-アミノ酸生産方法 | |
RU2811953C1 (ru) | Новый промотор и его применение | |
RU2824668C1 (ru) | Микроорганизм рода Corynebacterium, продуцирующий L-аминокислоты, и способ получения L-аминокислот с его использованием | |
JP7422888B2 (ja) | 新規なプロモーター及びその用途 | |
BR112021021219B1 (pt) | Micro-organismo com capacidade de produção de lhistidina melhorada de corynebacterium glutamicum, método para produzir l-histidina com o uso do mesmo e composição para produzir l-histidina | |
BR112021018712B1 (pt) | Micro-organismo produtor de l-treonina do gênero corynebacterium glutamicum, composição e método para produzir l-treonina | |
KR20220157144A (ko) | 신규 프로모터 및 이의 용도 | |
BR112021025702B1 (pt) | Polipeptídeo modificado tendo atividade de meso-diaminopimelato desidrogenase, polinucleotídeo, microrganismo do gênero corynebacterium e método para preparar l-treonina | |
BR112019009942B1 (pt) | Micro-organismo do gênero corynebacterium que produz l-lisina e método para produzir l-lisina |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
B09A | Decision: intention to grant [chapter 9.1 patent gazette] | ||
B16A | Patent or certificate of addition of invention granted [chapter 16.1 patent gazette] |
Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 17/06/2020, OBSERVADAS AS CONDICOES LEGAIS |