BR112021015080B1 - Micro-organismo produtor de l-tirosina, método para produzir ltirosina com o uso do mesmo, cassete de expressão e composição para produzir l-tirosina - Google Patents

Micro-organismo produtor de l-tirosina, método para produzir ltirosina com o uso do mesmo, cassete de expressão e composição para produzir l-tirosina Download PDF

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BR112021015080B1
BR112021015080B1 BR112021015080-3A BR112021015080A BR112021015080B1 BR 112021015080 B1 BR112021015080 B1 BR 112021015080B1 BR 112021015080 A BR112021015080 A BR 112021015080A BR 112021015080 B1 BR112021015080 B1 BR 112021015080B1
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tyrosine
gene
microorganism
trp
promoter
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BR112021015080-3A
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BR112021015080A2 (US06244707-20010612-C00010.png
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Gyuhyeon SONG
Chang Il Seo
Nara KWON
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Cj Cheiljedang Corporation
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00Preparation of nitrogen-containing organic compounds
    • C12P13/04Alpha- or beta- amino acids
    • C12P13/22Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
    • C12P13/225Tyrosine; 3,4-Dihydroxyphenylalanine
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88Lyases (4.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli
    • C12N15/71Expression systems using regulatory sequences derived from the trp-operon
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/001Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • CCHEMISTRY; METALLURGY
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    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00Preparation of nitrogen-containing organic compounds
    • C12P13/04Alpha- or beta- amino acids
    • C12P13/22Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y402/00Carbon-oxygen lyases (4.2)
    • C12Y402/01Hydro-lyases (4.2.1)
    • C12Y402/01051Prephenate dehydratase (4.2.1.51)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y504/00Intramolecular transferases (5.4)
    • C12Y504/99Intramolecular transferases (5.4) transferring other groups (5.4.99)
    • C12Y504/99005Chorismate mutase (5.4.99.5)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y103/00Oxidoreductases acting on the CH-CH group of donors (1.3)
    • C12Y103/01Oxidoreductases acting on the CH-CH group of donors (1.3) with NAD+ or NADP+ as acceptor (1.3.1)
    • C12Y103/01013Prephenate dehydrogenase (NADP+) (1.3.1.13)

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
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  • Wood Science & Technology (AREA)
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  • General Health & Medical Sciences (AREA)
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  • Biomedical Technology (AREA)
  • Microbiology (AREA)
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  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
BR112021015080-3A 2019-06-17 2020-06-17 Micro-organismo produtor de l-tirosina, método para produzir ltirosina com o uso do mesmo, cassete de expressão e composição para produzir l-tirosina BR112021015080B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR1020190071797A KR102134418B1 (ko) 2019-06-17 2019-06-17 L-타이로신을 생산하는 미생물 및 이를 이용한 l-타이로신 생산 방법
KR10-2019-0071797 2019-06-17
PCT/KR2020/007858 WO2020256415A1 (ko) 2019-06-17 2020-06-17 L-타이로신을 생산하는 미생물 및 이를 이용한 l-타이로신 생산 방법

Publications (2)

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BR112021015080A2 BR112021015080A2 (US06244707-20010612-C00010.png) 2021-12-28
BR112021015080B1 true BR112021015080B1 (pt) 2024-01-09

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BR112021015080-3A BR112021015080B1 (pt) 2019-06-17 2020-06-17 Micro-organismo produtor de l-tirosina, método para produzir ltirosina com o uso do mesmo, cassete de expressão e composição para produzir l-tirosina

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US (1) US20220195470A1 (US06244707-20010612-C00010.png)
EP (1) EP3901265A4 (US06244707-20010612-C00010.png)
JP (1) JP7267441B2 (US06244707-20010612-C00010.png)
KR (1) KR102134418B1 (US06244707-20010612-C00010.png)
CN (1) CN114072492B (US06244707-20010612-C00010.png)
AR (1) AR119176A1 (US06244707-20010612-C00010.png)
AU (1) AU2020295264B2 (US06244707-20010612-C00010.png)
BR (1) BR112021015080B1 (US06244707-20010612-C00010.png)
CA (1) CA3127994A1 (US06244707-20010612-C00010.png)
WO (1) WO2020256415A1 (US06244707-20010612-C00010.png)

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* Cited by examiner, † Cited by third party
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MX2022011083A (es) * 2021-05-20 2023-03-16 Cj Cheiljedang Corp Promotor novedoso y uso del mismo.
KR102685904B1 (ko) 2021-05-20 2024-07-19 씨제이제일제당 주식회사 신규 프로모터 및 이의 용도

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JP7267441B2 (ja) 2023-05-01
CN114072492A (zh) 2022-02-18
EP3901265A4 (en) 2022-03-30
JP2022521384A (ja) 2022-04-07
BR112021015080A2 (US06244707-20010612-C00010.png) 2021-12-28
AR119176A1 (es) 2021-12-01
US20220195470A1 (en) 2022-06-23
AU2020295264A1 (en) 2021-08-12
CN114072492B (zh) 2024-04-30
WO2020256415A1 (ko) 2020-12-24
EP3901265A1 (en) 2021-10-27
AU2020295264B2 (en) 2023-05-18
KR102134418B1 (ko) 2020-07-16
CA3127994A1 (en) 2020-12-24

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