ATE97441T1 - Verbesserung der expression und sekretion heterologener proteine in hefe mittels trunkierter sequenzen des alpha-faktor-leaders. - Google Patents

Verbesserung der expression und sekretion heterologener proteine in hefe mittels trunkierter sequenzen des alpha-faktor-leaders.

Info

Publication number
ATE97441T1
ATE97441T1 AT88312306T AT88312306T ATE97441T1 AT E97441 T1 ATE97441 T1 AT E97441T1 AT 88312306 T AT88312306 T AT 88312306T AT 88312306 T AT88312306 T AT 88312306T AT E97441 T1 ATE97441 T1 AT E97441T1
Authority
AT
Austria
Prior art keywords
yeast
encodes
precursor polypeptide
precursor
protein
Prior art date
Application number
AT88312306T
Other languages
English (en)
Inventor
Patricia Tekamp-Olson
Original Assignee
Chiron Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chiron Corp filed Critical Chiron Corp
Application granted granted Critical
Publication of ATE97441T1 publication Critical patent/ATE97441T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Diabetes (AREA)
  • General Engineering & Computer Science (AREA)
  • Endocrinology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Toxicology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
AT88312306T 1987-12-30 1988-12-23 Verbesserung der expression und sekretion heterologener proteine in hefe mittels trunkierter sequenzen des alpha-faktor-leaders. ATE97441T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US13968287A 1987-12-30 1987-12-30

Publications (1)

Publication Number Publication Date
ATE97441T1 true ATE97441T1 (de) 1993-12-15

Family

ID=22487825

Family Applications (1)

Application Number Title Priority Date Filing Date
AT88312306T ATE97441T1 (de) 1987-12-30 1988-12-23 Verbesserung der expression und sekretion heterologener proteine in hefe mittels trunkierter sequenzen des alpha-faktor-leaders.

Country Status (15)

Country Link
US (2) US5602034A (de)
EP (1) EP0324274B1 (de)
JP (1) JP2793215B2 (de)
AT (1) ATE97441T1 (de)
AU (1) AU616228B2 (de)
CA (1) CA1340772C (de)
DE (1) DE3885728T2 (de)
DK (2) DK175910B1 (de)
ES (1) ES2059547T3 (de)
FI (1) FI100995B (de)
HK (1) HK139994A (de)
IE (1) IE62087B1 (de)
IL (1) IL88802A0 (de)
NO (1) NO178829C (de)
PT (1) PT89367B (de)

Families Citing this family (102)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5170964A (ja) * 1974-12-16 1976-06-19 Hohnen Oil Haisuinoshoriho
DE3486216T2 (de) 1983-04-25 1994-02-17 Chiron Corp Hybrid-DNS-Synthesis von reifen insulinähnlichen Wachstumsfaktoren.
US5010182A (en) * 1987-07-28 1991-04-23 Chiron Corporation DNA constructs containing a Kluyveromyces alpha factor leader sequence for directing secretion of heterologous polypeptides
CA2090969C (en) * 1990-09-04 2001-08-21 Russell Arthur Brierley Production of insulin-like growth factor-1 in methylotrophic yeast cells
US5612198A (en) * 1990-09-04 1997-03-18 The Salk Institute Production of insulin-like growth factor-1 in methylotrophic yeast cells
DE69130981D1 (de) * 1990-09-14 1999-04-15 Chiron Corp Expression von makrophagen-induzierbaren proteinen (mips) in hefezellen
EP0785273A1 (de) 1990-11-26 1997-07-23 Genetics Institute, Inc. Gepaarte basische Aminosäuren konvertierendes Enzym und dafür kodierende DNA Sequenz
DK300090D0 (da) * 1990-12-19 1990-12-19 Novo Nordisk As Fremgangsmaade til fremstilling af leadersekvenser
JPH06506117A (ja) * 1991-04-01 1994-07-14 メルク　エンド　カンパニー　インコーポレーテッド ピキア（Ｐｉｃｈｉａ）蛋白質分解活性に影響する遺伝子およびその使用
US6348327B1 (en) 1991-12-06 2002-02-19 Genentech, Inc. Non-endocrine animal host cells capable of expressing variant proinsulin and processing the same to form active, mature insulin and methods of culturing such cells
AU6139794A (en) * 1993-02-10 1994-08-29 Unilever Plc Immobilized proteins with specific binding capacities and their use in processes and products
US5538863A (en) * 1993-07-01 1996-07-23 Immunex Corporation Expression system comprising mutant yeast strain and expression vector encoding synthetic signal peptide
US6451562B1 (en) 1993-12-22 2002-09-17 Human Genome Sciences, Inc. Polypeptides encoding myeloid progenitor inhibitory factor-1 (MPIF-1) polynucleotides
US6495129B1 (en) 1994-03-08 2002-12-17 Human Genome Sciences, Inc. Methods of inhibiting hematopoietic stem cells using human myeloid progenitor inhibitory factor-1 (MPIF-1) (Ckbeta-8/MIP-3)
US5629167A (en) 1994-04-19 1997-05-13 Biocine S.P.A. Detection of antibodies against Chlamydia trachomatis pgp3 antigen in patient sera by enzyme-linked immunosorbent assay
RU2167939C2 (ru) * 1994-06-16 2001-05-27 Ново Нордиск А/С Способ продуцирования полипептида в дрожжах
US5639642A (en) * 1994-06-16 1997-06-17 Novo Nordisk A/S Synthetic leader peptide sequences
US6500645B1 (en) 1994-06-17 2002-12-31 Novo Nordisk A/S N-terminally extended proteins expressed in yeast
IL114160A (en) * 1994-06-17 2006-12-31 Novo Nordisk As Dna constructs encoding heterologous proteins and processes for the heterologous protein production in yeast
HRP940432B1 (en) * 1994-08-05 2003-10-31 Pliva Pharm & Chem Works Dna sequences encoding biosynthetic insulin precursors and process for preparation of insulin
JPH10504971A (ja) * 1994-09-08 1998-05-19 カイロン コーポレイション インスリン様増殖因子の改善された生産方法
GB9513967D0 (en) * 1995-07-08 1995-09-06 Univ Leicester Insulin
ZA9610456B (en) * 1995-12-20 1997-06-20 Novo Nordisk As N-terminally extended proteins expressed in yeast
DE69731960T2 (de) * 1996-09-24 2005-12-15 Cadus Pharmaceutical Corp. Verfahren und zusammensetzungen für die identifizierung von receptor effectoren
WO1998013473A1 (en) 1996-09-25 1998-04-02 Vanderbilt University Genetically engineered yeast with modified signal peptidase complex
WO1998026080A1 (en) * 1996-12-13 1998-06-18 Chiron Corporation Method for expression of heterologous proteins in yeast
KR100381494B1 (ko) * 1997-06-30 2003-07-22 바이오 테크놀로지 제네랄 코오포레이션 인간인슐린의제조방법
US6068993A (en) * 1997-07-02 2000-05-30 Biobras Sa Vector for expression of heterologous protein and methods for extracting recombinant protein and for purifying isolated recombinant insulin
EP1003889A1 (de) 1997-08-05 2000-05-31 Chiron Corporation Gensequenzen aus pichia pastoris und deren verwendung
EP1900818A3 (de) 1997-11-06 2008-06-11 Novartis Vaccines and Diagnostics S.r.l. Neissersche Antigene
WO1999036544A2 (en) 1998-01-14 1999-07-22 Chiron S.P.A. Neisseria meningitidis antigens
WO1999037793A1 (en) 1998-01-23 1999-07-29 Novo Nordisk A/S Process for making desired polypeptides in yeast
GB9808932D0 (en) 1998-04-27 1998-06-24 Chiron Spa Polyepitope carrier protein
EP2261347A3 (de) 1998-05-01 2012-01-11 Novartis Vaccines and Diagnostics, Inc. Antigene und Zusammensetzungen gegen Neisseria meningitidis
AU5504999A (en) * 1998-07-20 2000-02-14 Frenken, Leon Gerardus Joseph Production of proteins
DE69932247T2 (de) 1998-08-21 2007-05-31 Immunex Corp., Thousand Oaks Menschliches il-1 epsilon dna und polypeptide
CA2372235A1 (en) 1999-04-30 2000-11-09 Rino Rappuoli Conserved neisserial antigens
GB9911683D0 (en) 1999-05-19 1999-07-21 Chiron Spa Antigenic peptides
GB9916529D0 (en) 1999-07-14 1999-09-15 Chiron Spa Antigenic peptides
EP1226253A2 (de) 1999-10-29 2002-07-31 Chiron S.P.A. Antigene peptide aus neisseria
CA2871789C (en) 2000-01-17 2017-04-04 Novartis Vaccines And Diagnostics S.R.L. Outer membrane vesicle (omv) vaccine comprising n. meningitidis serogroup b outer membrane proteins
DK1265630T3 (da) 2000-03-24 2006-10-09 Genentech Inc Anvendelse af insulin til behandling af brusksygdomme
WO2001090385A2 (en) 2000-05-25 2001-11-29 Chiron Corporation IRON REGULATION OF THE PpSEC10 TRANSCRIPTIONAL REGULATORY REGION OF PICHIA PASTORIS AND METHODS OF USE
US7132273B1 (en) 2000-07-26 2006-11-07 Korea Research Institute Of Bioscience And Biotechnology Cell wall anchor proteins derived from yeast, genes thereof and cell surface expression systems using the same
ATE439372T1 (de) 2000-10-27 2009-08-15 Novartis Vaccines & Diagnostic Nukleinsäuren und proteine von gruppen a und b- streptokokken
GB0107658D0 (en) 2001-03-27 2001-05-16 Chiron Spa Streptococcus pneumoniae
GB0107661D0 (en) 2001-03-27 2001-05-16 Chiron Spa Staphylococcus aureus
KR101054735B1 (ko) * 2001-07-06 2011-08-05 잇판자이단호진 가가쿠오요비겟세이료호겐쿠쇼 유전자 재조합 에카린 및 그를 제조하는 방법
EP2168596B1 (de) 2001-12-12 2012-05-23 Novartis Vaccines and Diagnostics S.r.l. Immunisierung gegen Chlamydia trachomatis
WO2003099854A2 (en) * 2002-05-24 2003-12-04 Nps Allelix Corp. Method for enzymatic production of glp-2(1-33) and glp-2-(1-34) peptides
AU2003239894A1 (en) * 2002-05-24 2003-12-12 Restoragen Inc. Methods and constructs for high yield expression of clostripain
DE60335497D1 (de) 2002-09-13 2011-02-03 Cornell Res Foundation Inc Ergillus-phytasen
WO2004046177A2 (en) 2002-11-15 2004-06-03 Chiron Srl Unexpected surface proteins in neisseria meningitidis
GB0308198D0 (en) 2003-04-09 2003-05-14 Chiron Srl ADP-ribosylating bacterial toxin
ATE554108T1 (de) 2003-07-25 2012-05-15 Amgen Inc Verfahren bezüglich ldcam und crtam
HRP20120315T1 (hr) * 2003-11-21 2012-05-31 Nps Pharmaceuticals Proizvodnja peptida 2 nalik glukagonu i analoga
EP2497478A3 (de) 2003-12-05 2012-11-07 Children's Hospital Medical Center Oligosaccarid-Zusammensetzungen und ihre Verwendung bei der Behandlung von Infektionen
AU2005319099B2 (en) * 2004-02-02 2010-09-16 Ambrx, Inc. Modified human growth hormone
CN103755800B (zh) 2004-02-02 2016-02-24 Ambrx公司 经修饰的人类生长激素多肽与其用途
CN102532321B (zh) 2004-06-18 2018-10-12 Ambrx公司 新颖抗原结合多肽和其用途
US7638299B2 (en) * 2004-07-21 2009-12-29 Ambrx, Inc. Biosynthetic polypeptides utilizing non-naturally encoded amino acids
AU2005323106B2 (en) 2004-12-22 2010-07-29 Ambrx, Inc. Methods for expression and purification of recombinant human growth hormone
US7385028B2 (en) 2004-12-22 2008-06-10 Ambrx, Inc Derivatization of non-natural amino acids and polypeptides
KR101224781B1 (ko) * 2004-12-22 2013-01-21 암브룩스, 인코포레이티드 비천연적으로 코딩된 아미노산을 포함하는 인간 성장호르몬의 조제물
NZ555425A (en) * 2004-12-22 2010-04-30 Ambrx Inc Compositions of aminoacyl-tRNA synthetase and uses thereof
WO2006132925A2 (en) 2005-06-01 2006-12-14 University Of Pittsburgh Of The Commonwealth System Of Higher Education Method of amidated peptide biosynthesis and delivery in vivo: endomorphin-2 for pain therapy
WO2006133088A2 (en) * 2005-06-03 2006-12-14 Ambrx, Inc. Improved human interferon molecules and their uses
CA2618878C (en) 2005-08-18 2016-06-28 Ambrx, Inc. Compositions of trna and uses thereof
SI1954710T1 (sl) * 2005-11-08 2011-08-31 Ambrx Inc Pospeĺ evala za modifikacijo nenaravnih aminokislin in nenaravnih peptidov aminokislin
EP1951890A4 (de) 2005-11-16 2009-06-24 Ambrx Inc Verfahren und zusammensetzungen mit nichtnatürlichen aminosäuren
KR101423898B1 (ko) * 2005-12-14 2014-07-28 암브룩스, 인코포레이티드 비-천연 아미노산 및 폴리펩티드를 함유하는 조성물, 비-천연 아미노산 및 폴리펩티드를 포함하는 방법, 및 비-천연 아미노산 및 폴리펩티드의 용도
ATE522541T1 (de) 2006-06-09 2011-09-15 Novartis Ag Bakterielle adhäsine konformere
PT2064333E (pt) * 2006-09-08 2014-06-09 Ambrx Inc Supressor híbrido arnt para células de vertebrados
ES2457527T3 (es) * 2006-09-08 2014-04-28 Ambrx, Inc. ARNt Supresor hibrido en células de vertebrados
WO2008030558A2 (en) * 2006-09-08 2008-03-13 Ambrx, Inc. Modified human plasma polypeptide or fc scaffolds and their uses
JPWO2008032659A1 (ja) * 2006-09-14 2010-01-21 独立行政法人産業技術総合研究所 効率向上型分泌シグナルペプチド及びそれらを利用したタンパク質生産方法
KR101476472B1 (ko) * 2007-03-30 2015-01-05 암브룩스, 인코포레이티드 변형된 ｆｇｆ-21 폴리펩티드 및 그 용도
KR20100019467A (ko) 2007-05-02 2010-02-18 암브룩스, 인코포레이티드 변형된 인터페론 베타 폴리펩티드 및 그의 용도
ES2632504T3 (es) 2007-11-20 2017-09-13 Ambrx, Inc. Polipéptidos de insulina modificados y sus usos
NZ602170A (en) 2008-02-08 2014-03-28 Ambrx Inc Modified leptin polypeptides and their uses
MX2011000859A (es) 2008-07-23 2011-02-24 Ambrx Inc Polipeptidos g-csf bovinos modificados y sus usos.
PL2342223T3 (pl) 2008-09-26 2017-09-29 Ambrx, Inc. Zmodyfikowane polipeptydy zwierzęcej erytropoetyny i ich zastosowania
NO2337846T3 (de) 2008-09-26 2018-06-16
EP2258855A1 (de) 2009-05-28 2010-12-08 Universität für Bodenkultur Wien Expressionssequenzen
US20120213728A1 (en) * 2009-10-30 2012-08-23 Merck Granulocyte-colony stimulating factor produced in glycoengineered pichia pastoris
NZ600361A (en) 2009-12-21 2014-06-27 Ambrx Inc Modified bovine somatotropin polypeptides and their uses
EP2516455A4 (de) 2009-12-21 2013-05-22 Ambrx Inc Modifizierte schweine-somatotropin-polypeptide und deren verwendungen
US9567386B2 (en) 2010-08-17 2017-02-14 Ambrx, Inc. Therapeutic uses of modified relaxin polypeptides
SMT201900495T1 (it) 2010-08-17 2019-11-13 Ambrx Inc Polipeptidi di relaxina modificati e loro usi
TWI480288B (zh) 2010-09-23 2015-04-11 Lilly Co Eli 牛顆粒細胞群落刺激因子及其變體之調配物
US20140234330A1 (en) 2011-07-22 2014-08-21 Amgen Inc. Il-17 receptor a is required for il-17c biology
EP2912053B1 (de) 2012-10-29 2016-11-09 Lonza Ltd Expressionssequenzen
AU2014260123B2 (en) 2013-04-30 2018-10-11 Donald Danforth Plant Science Center Antifungal plant proteins, peptides, and methods of use
CN106029688A (zh) 2014-02-28 2016-10-12 诺和诺德股份有限公司 交配因子α原肽变体
BR112017008157A2 (pt) 2014-10-24 2017-12-19 Bristol Myers Squibb Co polipeptídeos de fgf-21 modificados e usos dos mesmos
US10266578B2 (en) 2017-02-08 2019-04-23 Bristol-Myers Squibb Company Modified relaxin polypeptides comprising a pharmacokinetic enhancer and uses thereof
US20210032307A1 (en) * 2018-02-09 2021-02-04 Jiangsu Hengrui Medicine Co., Ltd. Codon optimized precursor gene and signal peptide gene of human insulin analogue
CN113015782A (zh) 2018-11-19 2021-06-22 巴斯夫欧洲公司 用于酵母的前导序列
CN110028562B (zh) * 2019-04-25 2023-03-03 福州量衍科技有限公司 优化的Komagataella pastoris酵母α-factor信号肽及应用
US12331302B2 (en) 2019-11-08 2025-06-17 The Regents Of The University Of California Fungal autoinducible expression system
BR102019026867A2 (pt) * 2019-12-16 2021-06-29 Petróleo Brasileiro S.A. - Petrobras Microrganismos geneticamente modificados que realizam a produção heteróloga de versões modificadas da proteína surfactante lv-ranaspumina-1 (lv-rsn-1), as versões modificadas dessa proteína surfactante, os genes sintéticos que codificam essa proteína surfactante, os cassetes de expressão contendo esses genes sintéticos, e os vetores de expressão contendo esses genes sintéticos
WO2026009000A1 (en) 2024-07-04 2026-01-08 Solasta Bio Limited Methods for production of insecticidal neuropeptides

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4366246A (en) 1977-11-08 1982-12-28 Genentech, Inc. Method for microbial polypeptide expression
IL63270A0 (en) 1980-08-05 1981-10-30 Univ Leland Stanford Junior Eukaryotic autonomously replicating segment
US4775622A (en) * 1982-03-08 1988-10-04 Genentech, Inc. Expression, processing and secretion of heterologous protein by yeast
US4546082A (en) * 1982-06-17 1985-10-08 Regents Of The Univ. Of California E. coli/Saccharomyces cerevisiae plasmid cloning vector containing the alpha-factor gene for secretion and processing of hybrid proteins
DE3382547D1 (de) 1983-01-12 1992-05-27 Chiron Corp Sekretorische expression in eukaryoten.
CA1341302C (en) 1983-02-22 2001-10-09 Rae Lyn Burke Yeast expression systems with vectors having gapdh or pyk promoters and synthesis of foreign protein
WO1984004330A1 (en) * 1983-04-22 1984-11-08 Amgen Secretion of exogenous polypeptides from yeast
US4546083A (en) 1983-04-22 1985-10-08 Stolle Research & Development Corporation Method and device for cell culture growth
NZ207926A (en) * 1983-04-25 1988-04-29 Genentech Inc Use of yeast #a#-factor to assist in expression of proteins heterologus to yeast
DE3486216T2 (de) * 1983-04-25 1994-02-17 Chiron Corp Hybrid-DNS-Synthesis von reifen insulinähnlichen Wachstumsfaktoren.
US4870008A (en) * 1983-08-12 1989-09-26 Chiron Corporation Secretory expression in eukaryotes
DE3587759T2 (de) 1984-05-11 1994-07-07 Chiron Corp., Emeryville, Calif. Erhöhte Hefetranskription unter Verwendung einer Hybridkonstruktion der Promotorregion.
JPS6156078A (ja) * 1984-07-27 1986-03-20 Suntory Ltd 酵母を宿主とする分泌発現ベクタ−
WO1986001515A1 (en) * 1984-08-29 1986-03-13 Chiron Corporation Expression of human connective tissue activator and related peptides
DK129385A (da) * 1985-03-22 1986-09-23 Novo Industri As Peptider og fremstilling deraf
JPS6236183A (ja) 1985-06-20 1987-02-17 ザ・サルク・インステイチユ−ト・バイオテクノロジ−／インダストリアル・アソシエイツ・インコ−ポレ−テツド サツカロミセス・セレビシエからのポリペプチドの発現および分泌
PH25772A (en) * 1985-08-30 1991-10-18 Novo Industri As Insulin analogues, process for their preparation
US5010182A (en) 1987-07-28 1991-04-23 Chiron Corporation DNA constructs containing a Kluyveromyces alpha factor leader sequence for directing secretion of heterologous polypeptides
AU633020B2 (en) * 1988-07-23 1993-01-21 Novozymes Delta Limited New secretory leader sequences

Also Published As

Publication number Publication date
EP0324274B1 (de) 1993-11-18
DK200500130A (da) 2005-01-27
DK175910B1 (da) 2005-06-13
NO885786L (no) 1989-07-03
JP2793215B2 (ja) 1998-09-03
EP0324274A1 (de) 1989-07-19
IE62087B1 (en) 1994-12-14
DK728588A (da) 1989-07-01
CA1340772C (en) 1999-09-28
PT89367B (pt) 1993-09-30
DE3885728T2 (de) 1994-03-10
NO885786D0 (no) 1988-12-28
NO178829C (no) 1996-06-12
US5602034A (en) 1997-02-11
PT89367A (pt) 1989-12-29
NO178829B (no) 1996-03-04
DE3885728D1 (de) 1993-12-23
IL88802A0 (en) 1989-07-31
FI100995B (fi) 1998-03-31
FI886008L (fi) 1989-07-01
USRE37343E1 (en) 2001-08-28
AU616228B2 (en) 1991-10-24
DK176075B1 (da) 2006-04-10
HK139994A (en) 1994-12-16
JPH022339A (ja) 1990-01-08
IE883896L (en) 1989-06-30
ES2059547T3 (es) 1994-11-16
DK728588D0 (da) 1988-12-29
AU2765088A (en) 1989-07-06

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