ME01366B - POSTUPAK ZA PROIZVODNJU PEPTIDA 2 NALIK NA GLUKAGON l NJIHOVIH ANALOGA - Google Patents
POSTUPAK ZA PROIZVODNJU PEPTIDA 2 NALIK NA GLUKAGON l NJIHOVIH ANALOGAInfo
- Publication number
- ME01366B ME01366B MEP-2012-46A MEP4612A ME01366B ME 01366 B ME01366 B ME 01366B ME P4612 A MEP4612 A ME P4612A ME 01366 B ME01366 B ME 01366B
- Authority
- ME
- Montenegro
- Prior art keywords
- multimer
- peptide
- glp
- enzyme
- cleavage site
- Prior art date
Links
- 101800000221 Glucagon-like peptide 2 Proteins 0.000 title claims abstract 11
- 102100040918 Pro-glucagon Human genes 0.000 title 1
- TWSALRJGPBVBQU-PKQQPRCHSA-N glucagon-like peptide 2 Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(O)=O)C(O)=O)[C@@H](C)CC)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCSC)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CO)NC(=O)[C@H](CC=1C=CC=CC=1)NC(=O)[C@H](CO)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](C)NC(=O)[C@@H](N)CC=1NC=NC=1)[C@@H](C)O)[C@@H](C)CC)C1=CC=CC=C1 TWSALRJGPBVBQU-PKQQPRCHSA-N 0.000 title 1
- 238000003776 cleavage reaction Methods 0.000 claims abstract 10
- 230000007017 scission Effects 0.000 claims abstract 10
- 108090000790 Enzymes Proteins 0.000 claims abstract 9
- 102000004190 Enzymes Human genes 0.000 claims abstract 9
- 239000002253 acid Substances 0.000 claims abstract 8
- 229940088598 enzyme Drugs 0.000 claims 8
- 238000000034 method Methods 0.000 claims 7
- 108090000623 proteins and genes Proteins 0.000 claims 6
- 102000004169 proteins and genes Human genes 0.000 claims 6
- 108020004414 DNA Proteins 0.000 claims 2
- 230000001413 cellular effect Effects 0.000 claims 2
- 108090000765 processed proteins & peptides Proteins 0.000 claims 2
- 238000000926 separation method Methods 0.000 claims 2
- OZRFYUJEXYKQDV-UHFFFAOYSA-N 2-[[2-[[2-[(2-amino-3-carboxypropanoyl)amino]-3-carboxypropanoyl]amino]-3-carboxypropanoyl]amino]butanedioic acid Chemical compound OC(=O)CC(N)C(=O)NC(CC(O)=O)C(=O)NC(CC(O)=O)C(=O)NC(CC(O)=O)C(O)=O OZRFYUJEXYKQDV-UHFFFAOYSA-N 0.000 claims 1
- UKGGPJNBONZZCM-WDSKDSINSA-N Asp-Pro Chemical compound OC(=O)C[C@H](N)C(=O)N1CCC[C@H]1C(O)=O UKGGPJNBONZZCM-WDSKDSINSA-N 0.000 claims 1
- 125000001433 C-terminal amino-acid group Chemical group 0.000 claims 1
- 101800001453 C-terminal extension peptide Proteins 0.000 claims 1
- 102000053602 DNA Human genes 0.000 claims 1
- 241000701832 Enterobacteria phage T3 Species 0.000 claims 1
- 108010013369 Enteropeptidase Proteins 0.000 claims 1
- 102100029727 Enteropeptidase Human genes 0.000 claims 1
- 241000588724 Escherichia coli Species 0.000 claims 1
- 108010074860 Factor Xa Proteins 0.000 claims 1
- 108090000190 Thrombin Proteins 0.000 claims 1
- 108010088665 Zinc Finger Protein Gli2 Proteins 0.000 claims 1
- 108010093581 aspartyl-proline Proteins 0.000 claims 1
- 239000007795 chemical reaction product Substances 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 108091033319 polynucleotide Proteins 0.000 claims 1
- 102000040430 polynucleotide Human genes 0.000 claims 1
- 239000002157 polynucleotide Substances 0.000 claims 1
- 229960004072 thrombin Drugs 0.000 claims 1
- 101100505076 Caenorhabditis elegans gly-2 gene Proteins 0.000 abstract 1
- 102400000326 Glucagon-like peptide 2 Human genes 0.000 abstract 1
- 210000004899 c-terminal region Anatomy 0.000 abstract 1
- YMAWOPBAYDPSLA-UHFFFAOYSA-N glycylglycine Chemical compound [NH3+]CC(=O)NCC([O-])=O YMAWOPBAYDPSLA-UHFFFAOYSA-N 0.000 abstract 1
- 238000002955 isolation Methods 0.000 abstract 1
- 239000002243 precursor Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/605—Glucagons
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Toxicology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Gastroenterology & Hepatology (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Endocrinology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Claims (14)
1.Jednolančani proteinski multimer koji obuhvata najmanje dve jedinice GLP-2 peptida spojednog dvostruko sa veznikom koji obezbeđuje u okviru multimera mesto kiselinskog razdvajanja na njegovom N-kraju, i mesto razdavajanja enzima na njgovom C-kraju, gde razdvajanje pomenutog multimera sa kiselinom i enzimom oslobađa pomenute jedinice GLP-2 peptida, od kojih svaka ima autentične N- i C-terminalne ostatke i gde kiselinsko mesto razdvajanja ima sekvencu Asp-Pro i mesto razdvajanja enzima je odabrano od mesta razdvajanja enterokinaze koje ima sekvencu Asp-Asp-Asp-Asp-Liz, mesto razdvajanja faktora Xa ima sekvencu Ile-Glu-Glu-Arg i mesto razdvajanja trombina ima sekvencu Val-Ser-Gli-Pro-Arg.
2.Jednolančani proteinski multimer prema zahtevu 1, gde se pomenuti veznik sastoji ne više od 25 ostataka, manje od 20 ostataka, manje od 15 ostataka, ili manje od 10 ostataka.
3.Jednolančani proteinski multimer prema zahtevu 1 ili 2, gde pomenuti veznik ima sekvencu ProValSerGliProArg.
4.Jednolančani proteinski multimer prema bilo kom od zahteva 1 do 3, gde je pomenuti GLP-2 peptid [Gli2]hGLP-2.
5.Jednolančani proteinski multimer prema bilo kom od zahteva 1 do 4, koji dalje sadrži noseći peptid vezan tako da se može osloboditi na njegovom N-kraju, noseći peptid obezbeđuje Met ostatak na njegovom N-kraju, a na njegovom C- kraju, mesto razdvajanja pomenutog enzima.
6.Jednolančani proteinski multimer prema bilo kom od zahteva 1 do 5, koji dalje sadrži C-terminalni ekstenzioni peptid. .
7.Postupak za pripremanje multimera prema bilo kom od zahteva 1 do 6, koji obuhvata korak kultivisanja ćelijskog domaćina koji inkorporira eksperesioni konstrukt u kojem je molekul DNK koji kodira pomenuti multimer operativno vezan sa DNK obezbeđujući njegovu ekspresiju.
8.Postupak prema zahtevu 7, gde je pomenuti domaćin E. coli domaćin.
9.Postupak prema bilo kom od zahteva 7 ili 8, gde ekspresioni konstrukt dalje obuhvata promotor T7 bakteriofaga.
10.Polinukleotid koji kodira multimer prema bilo kom od zahteva 1 - 6.
11.Postupak za pripremanje GLP-2 peptida, koji obuhvata korake dobijanja multimera prema bilo kom od zahteva 1 do 6, tretiranje multimera sa kiselinom i sa enzimom da se odvoji veznik koji je tu smešten, i zatim izolovanja nastale GLP-2 peptidne jedinice.
12.Postupak prema zahtevu 11, gde je multimer prvo odvojen sa kiselinom, nastali odvojeni multimer je izolovan a zatim odvojen sa enzimom, i zatim su izolovane nastale GLP-2 peptidne jedinice koje imaju autentične krajeve.
13.Postupak prema zahtevu 12, gde je korak razdvajanja kiselinom izveden u vreme ekstrahovanja GLP-2 peptidnog multimera od ćelijskog domaćina produkujući tako pomenuti multimer.
14. Postupak prema zahtevu 11, gde je korak tretiranja multimera sa kiselinom i sa enzimom izveden bez razdvajanja reakcionih produkata pre tretmana enzimom.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US52366703P | 2003-11-21 | 2003-11-21 | |
| EP04821092A EP1704234B1 (en) | 2003-11-21 | 2004-11-22 | Production of glucagon like peptide 2 and analogs |
| PCT/IB2004/004439 WO2005067368A2 (en) | 2003-11-21 | 2004-11-22 | Production of glucagon like peptide 2 and analogs |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ME01366B true ME01366B (me) | 2013-12-20 |
Family
ID=34794202
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MEP-2012-46A ME01366B (me) | 2003-11-21 | 2004-11-22 | POSTUPAK ZA PROIZVODNJU PEPTIDA 2 NALIK NA GLUKAGON l NJIHOVIH ANALOGA |
Country Status (13)
| Country | Link |
|---|---|
| US (2) | US7829307B2 (me) |
| EP (1) | EP1704234B1 (me) |
| AT (1) | ATE541932T1 (me) |
| CA (1) | CA2546319A1 (me) |
| DK (1) | DK1704234T3 (me) |
| ES (1) | ES2380765T3 (me) |
| HR (1) | HRP20120315T1 (me) |
| ME (1) | ME01366B (me) |
| PL (1) | PL1704234T3 (me) |
| PT (1) | PT1704234E (me) |
| RS (1) | RS52246B (me) |
| SI (1) | SI1704234T1 (me) |
| WO (1) | WO2005067368A2 (me) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101895047B1 (ko) | 2011-12-30 | 2018-09-06 | 한미사이언스 주식회사 | 면역글로불린 단편을 이용한 위치 특이적 글루카곤 유사 펩타이드-2 약물 결합체 |
| TWI591177B (zh) | 2016-10-27 | 2017-07-11 | 中化合成生技股份有限公司 | 一種胰高血糖素胜肽－２（ｇｌｐ－２）類似物的製備方法 |
| JP2020513834A (ja) | 2017-03-20 | 2020-05-21 | ルピン・リミテッド | ペプチドの発現および大規模生産 |
| EP4110788A4 (en) | 2020-02-28 | 2024-08-28 | Kallyope, Inc. | Gpr40 agonists |
| WO2021236617A1 (en) | 2020-05-19 | 2021-11-25 | Kallyope, Inc. | Ampk activators |
| AU2021297323A1 (en) | 2020-06-26 | 2023-02-16 | Kallyope, Inc. | AMPK activators |
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-
2004
- 2004-11-22 US US10/993,127 patent/US7829307B2/en not_active Expired - Fee Related
- 2004-11-22 PL PL04821092T patent/PL1704234T3/pl unknown
- 2004-11-22 SI SI200431853T patent/SI1704234T1/sl unknown
- 2004-11-22 ME MEP-2012-46A patent/ME01366B/me unknown
- 2004-11-22 DK DK04821092.6T patent/DK1704234T3/da active
- 2004-11-22 PT PT04821092T patent/PT1704234E/pt unknown
- 2004-11-22 AT AT04821092T patent/ATE541932T1/de active
- 2004-11-22 HR HRP20120315TT patent/HRP20120315T1/hr unknown
- 2004-11-22 ES ES04821092T patent/ES2380765T3/es not_active Expired - Lifetime
- 2004-11-22 WO PCT/IB2004/004439 patent/WO2005067368A2/en active Application Filing
- 2004-11-22 RS RS20120152A patent/RS52246B/en unknown
- 2004-11-22 CA CA002546319A patent/CA2546319A1/en not_active Abandoned
- 2004-11-22 EP EP04821092A patent/EP1704234B1/en not_active Expired - Lifetime
-
2010
- 2010-09-30 US US12/895,517 patent/US20110092424A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| EP1704234B1 (en) | 2012-01-18 |
| US20050164930A1 (en) | 2005-07-28 |
| RS52246B (en) | 2012-10-31 |
| HRP20120315T1 (hr) | 2012-05-31 |
| US20110092424A1 (en) | 2011-04-21 |
| DK1704234T3 (da) | 2012-05-07 |
| EP1704234A2 (en) | 2006-09-27 |
| ES2380765T3 (es) | 2012-05-18 |
| WO2005067368A2 (en) | 2005-07-28 |
| PT1704234E (pt) | 2012-04-26 |
| PL1704234T3 (pl) | 2012-06-29 |
| EP1704234A4 (en) | 2007-11-07 |
| ATE541932T1 (de) | 2012-02-15 |
| CA2546319A1 (en) | 2005-07-28 |
| WO2005067368A3 (en) | 2006-03-23 |
| US7829307B2 (en) | 2010-11-09 |
| SI1704234T1 (sl) | 2012-08-31 |
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