ATE461936T1 - Affinitätsliganden - Google Patents

Affinitätsliganden

Info

Publication number
ATE461936T1
ATE461936T1 AT06721225T AT06721225T ATE461936T1 AT E461936 T1 ATE461936 T1 AT E461936T1 AT 06721225 T AT06721225 T AT 06721225T AT 06721225 T AT06721225 T AT 06721225T AT E461936 T1 ATE461936 T1 AT E461936T1
Authority
AT
Austria
Prior art keywords
polypeptide
interest
fusion
inclusion bodies
heterologous
Prior art date
Application number
AT06721225T
Other languages
English (en)
Inventor
Alois Jungbauer
Rainer Hahn
Waltraud Kaar
Michael Seifert
Bernhard Auer
Clemens Achmueller
Philipp Wechner
Original Assignee
Sandoz Ag
Boehringer Ingelheim Rcv Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB0508435.5A external-priority patent/GB0508435D0/en
Priority claimed from GBGB0508434.8A external-priority patent/GB0508434D0/en
Priority claimed from GB0605379A external-priority patent/GB0605379D0/en
Application filed by Sandoz Ag, Boehringer Ingelheim Rcv Gmbh filed Critical Sandoz Ag
Application granted granted Critical
Publication of ATE461936T1 publication Critical patent/ATE461936T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K17/00—Carrier-bound or immobilised peptides; Preparation thereof
    • C07K17/02—Peptides being immobilised on, or in, an organic carrier
    • C07K17/06—Peptides being immobilised on, or in, an organic carrier attached to the carrier via a bridging agent
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/503—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses
    • C12N9/506—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses derived from RNA viruses

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
AT06721225T 2005-04-26 2006-04-25 Affinitätsliganden ATE461936T1 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GBGB0508435.5A GB0508435D0 (en) 2005-04-26 2005-04-26 Organic compounds
GBGB0508434.8A GB0508434D0 (en) 2005-04-26 2005-04-26 Organic compounds
GB0605379A GB0605379D0 (en) 2006-03-16 2006-03-16 Organic compounds
PCT/AT2006/000167 WO2006113958A2 (en) 2005-04-26 2006-04-25 Affinity ligands

Publications (1)

Publication Number Publication Date
ATE461936T1 true ATE461936T1 (de) 2010-04-15

Family

ID=36796250

Family Applications (1)

Application Number Title Priority Date Filing Date
AT06721225T ATE461936T1 (de) 2005-04-26 2006-04-25 Affinitätsliganden

Country Status (15)

Country Link
US (4) US8058410B2 (de)
EP (5) EP2348053A3 (de)
JP (5) JP2008539170A (de)
KR (3) KR101374149B1 (de)
CN (1) CN103333252A (de)
AT (1) ATE461936T1 (de)
AU (3) AU2006239721B2 (de)
CA (3) CA2605149A1 (de)
DE (1) DE602006013111D1 (de)
DK (3) DK1874825T3 (de)
ES (2) ES2399031T3 (de)
PL (1) PL1874825T3 (de)
PT (2) PT1874932E (de)
SI (2) SI1874825T1 (de)
WO (3) WO2006113959A2 (de)

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EP2348053A3 (de) * 2005-04-26 2011-11-02 Sandoz AG Oligopeptid-Liganden
JP5837747B2 (ja) 2007-05-17 2015-12-24 ベーリンガー インゲルハイム エルツェーファウ ゲゼルシャフト ミット ベシュレンクテル ハフツング ウント コンパニー コマンディトゲゼルシャフト 組換えタンパク質を工業規模で製造する方法
MX2010004803A (es) * 2007-10-31 2010-09-09 Diffusion Pharmaceuticals Llc Una nueva clase de composiciones terapeuticas que mejoran la difusion de moleculas pequeñas.
EP2130912A1 (de) * 2008-06-04 2009-12-09 Institut für Viruskrankeiten und Immunprophylaxe Ein RNA-basiertes virales Vektorsystem bereitstellende Pestivirusreplikone
US20120171184A1 (en) 2010-12-31 2012-07-05 Lajos Szente Cellular hydration compositions
EP2672265B1 (de) * 2011-01-12 2017-08-02 Sekisui Medical Co., Ltd. Eluierungsmittel für die ionenaustauschchromatographie und verfahren zur analyse von nukleinsäureketten
US20140147842A1 (en) 2011-01-12 2014-05-29 Sekisui Medical Co. Ltd Method for detecting single nucleotide polymorphisms
WO2012098449A1 (en) * 2011-01-17 2012-07-26 University Of Manitoba Methods for treating disorders that involve immunoglobulin a
EP2684951A1 (de) 2012-07-13 2014-01-15 Sandoz Ag Verfahren zur Herstellung eines rekombinanten Proteins von Interesse
EP2746390A1 (de) * 2012-12-19 2014-06-25 Sandoz Ag Verfahren zur Herstellung eines rekombinanten Proteins von Interesse
EP2746391A1 (de) * 2012-12-19 2014-06-25 Sandoz Ag Verfahren zur Herstellung eines rekombinanten Proteins von Interesse
UY35874A (es) 2013-12-12 2015-07-31 Novartis Ag Un proceso para la preparación de una composición de proteínas pegiladas
US11054425B2 (en) 2014-12-19 2021-07-06 Roche Sequencing Solutions, Inc. System and method for identification and characterization of transglutaminase species
CN107406483B (zh) * 2014-12-19 2022-02-22 豪夫迈·罗氏有限公司 微生物转谷氨酰胺酶，其底物和其使用方法
AU2016233557B2 (en) * 2015-03-13 2021-06-24 Bristol-Myers Squibb Company Use of alkaline washes during chromatography to remove impurities
CN104844685B (zh) * 2015-06-12 2018-10-16 中国科学院植物研究所 一种变性抗原亲和纯化抗体方法
RU2619217C1 (ru) * 2015-12-04 2017-05-12 Федеральное государственное бюджетное учреждение "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов" (ФГБУ "ГосНИИгенетика") Температурочувствительный мутантный интеин для нерастворимой экспрессии предшественника целевого белка
KR101830792B1 (ko) 2016-01-27 2018-02-21 건국대학교 산학협력단 항균 펩타이드를 포함하는 불용성 융합단백질 및 이를 이용한 항균 펩타이드의 제조 방법
CN110945123B (zh) * 2017-06-23 2023-06-23 珠海亿胜生物制药有限公司 生产可溶性重组人碱性成纤维细胞生长因子(rh-bFGF)的方法
DE102018200602A1 (de) 2018-01-15 2019-07-18 Technische Universität München Biologische Synthese von Aminosäureketten zur Herstellung von Peptiden und Proteinen
CN110343183B (zh) * 2018-04-03 2023-05-12 点斗基因科技(南京)有限公司 一种重组表达载体及其构建方法和应用
CN111019962A (zh) * 2019-12-18 2020-04-17 南京理工大学 一种sod-elp融合蛋白及其制备方法
EP3904525A1 (de) 2020-04-27 2021-11-03 Kutzner, Christoph Fusionspolypeptide für die zielpeptid-produktion
CN114539425B (zh) * 2022-02-25 2023-04-28 湖南中晟全肽生化有限公司 一种提高线性多肽生物表达的方法
EP4265636A1 (de) 2022-04-19 2023-10-25 mk2 Biotechnologies GmbH Herstellung von zielpeptiden und -proteinen

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AU4946297A (en) * 1996-10-09 1998-05-05 Akzo Nobel N.V. A composition comprising an immobilised, acylated peptide
AU9341398A (en) * 1997-08-22 1999-03-16 Boehringer Mannheim Gmbh Protease precursors that can be autocatalytically activated and their use
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US5985836A (en) * 1998-07-31 1999-11-16 Bayer Corporation Alpha-1 proteinase inhibitor binding peptides
JP4820512B2 (ja) 1999-08-09 2011-11-24 サンド・アクチエンゲゼルシヤフト タンパク質の製造
JP5480458B2 (ja) * 1999-08-09 2014-04-23 サンド・アクチエンゲゼルシヤフト 自己タンパク質分解切断によるタンパク質の製造
JP4202574B2 (ja) * 2000-01-11 2008-12-24 Ａｓｐｉｏｎ株式会社 ｇｐ１２０に親和性を有するぺプチド
AU2001253758A1 (en) * 2000-04-24 2001-11-07 Yale University DNA and protein binding miniature proteins
ATE419333T1 (de) * 2001-02-06 2009-01-15 Massachusetts Inst Technology Peptidgerüstverkapselung von gewebszellen und verwendungen davon
DK1390535T3 (da) * 2001-04-26 2010-12-06 Amgen Mountain View Inc Kombinatoriske biblioteker af monomer-domæner
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WO2004043997A2 (en) 2002-11-12 2004-05-27 Yeda Research And Development Co. Ltd. Chimeric autoprocessing polypeptides and uses thereof
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EP2348053A3 (de) * 2005-04-26 2011-11-02 Sandoz AG Oligopeptid-Liganden
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Also Published As

Publication number Publication date
JP2015226547A (ja) 2015-12-17
EP2348053A3 (de) 2011-11-02
WO2006113957A2 (en) 2006-11-02
KR20080036547A (ko) 2008-04-28
US20100062490A1 (en) 2010-03-11
US7829319B2 (en) 2010-11-09
CA2605140C (en) 2014-06-17
WO2006113959A2 (en) 2006-11-02
WO2006113957A3 (en) 2007-06-07
JP5909172B2 (ja) 2016-04-26
US8163890B2 (en) 2012-04-24
AU2006239721B2 (en) 2011-07-14
DK1874933T3 (en) 2015-03-02
PT1874825E (pt) 2010-06-22
US8372959B2 (en) 2013-02-12
WO2006113957A8 (en) 2007-02-22
SI1874932T1 (sl) 2013-02-28
EP1874825B1 (de) 2010-03-24
EP1874932B1 (de) 2012-10-17
US8058410B2 (en) 2011-11-15
WO2006113959A3 (en) 2007-03-22
AU2006239723A1 (en) 2006-11-02
PL1874825T3 (pl) 2011-04-29
EP2366719A3 (de) 2012-03-07
CA2605140A1 (en) 2006-11-02
JP2013013419A (ja) 2013-01-24
DK1874932T3 (da) 2013-02-04
AU2006239723B2 (en) 2011-12-22
WO2006113958A8 (en) 2007-01-18
KR20080036545A (ko) 2008-04-28
CA2605145A1 (en) 2006-11-02
SI1874825T1 (sl) 2010-08-31
AU2006239722A1 (en) 2006-11-02
KR101289728B1 (ko) 2013-08-07
EP1874933B1 (de) 2014-11-19
CA2605149A1 (en) 2006-11-02
US20090203069A1 (en) 2009-08-13
AU2006239722B2 (en) 2012-02-02
WO2006113958A3 (en) 2007-05-03
ES2342100T3 (es) 2010-07-01
EP2348053A2 (de) 2011-07-27
EP1874933A2 (de) 2008-01-09
DK1874825T3 (da) 2010-07-05
CN103333252A (zh) 2013-10-02
JP5101492B2 (ja) 2012-12-19
US20120107869A1 (en) 2012-05-03
PT1874932E (pt) 2013-01-24
KR101374149B1 (ko) 2014-03-14
JP2008539171A (ja) 2008-11-13
AU2006239721A1 (en) 2006-11-02
EP1874825A2 (de) 2008-01-09
KR20080030551A (ko) 2008-04-04
EP2366719B1 (de) 2018-02-21
WO2006113958A2 (en) 2006-11-02
ES2399031T3 (es) 2013-03-25
EP1874932A2 (de) 2008-01-09
US20090306343A1 (en) 2009-12-10
JP2008539170A (ja) 2008-11-13
JP2008538897A (ja) 2008-11-13
EP2366719A2 (de) 2011-09-21
DE602006013111D1 (de) 2010-05-06

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