KR860006484A - 단백질 또는 당단백질의 제조방법 - Google Patents

단백질 또는 당단백질의 제조방법 Download PDF

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Publication number
KR860006484A
KR860006484A KR1019860000867A KR860000867A KR860006484A KR 860006484 A KR860006484 A KR 860006484A KR 1019860000867 A KR1019860000867 A KR 1019860000867A KR 860000867 A KR860000867 A KR 860000867A KR 860006484 A KR860006484 A KR 860006484A
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South Korea
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cdna
protein
ser
amino acid
acid sequence
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KR1019860000867A
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KR940003690B1 (ko
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라그 헤르만
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훽스트 아크티엔게젤샤프트
하인리히 벡커, 베른하르트 벡크
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Publication of KR860006484A publication Critical patent/KR860006484A/ko
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Publication of KR940003690B1 publication Critical patent/KR940003690B1/ko

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
    • C07K14/8121—Serpins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

내용 없음

Description

단백질 또는 당단백질의 제조방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (8)

  1. 제 1 도의 아미노산 서열 Ⅰ의 단백질에 대해 코오드화 된 cDNA를 발현 시스템에 도입시켜 시스템 내에서 발현시킴을 특징으로 하여, 단백질 또는 당단백질을 제조하는 방법.
  2. 제 1 항에 있어서, cDNA를 DNA 서열 Ⅱ 5' GGGTTGGCTACTCTGCCCATGAAGA 3'의 프로우브(probe)를 사용하여 인체의 간생검 물질의 cDNA 유전자 뱅크(bank)를 선별함으로써 수득하는 방법.
  3. 제 1 또는 2항에 있어서, cDNA가 제2도의 DNA 서열Ⅰ을 함유하는 방법.
  4. 제 1 내지 3항 중의 어느 한 항에 있어서, cDNA가, Arg－Ser, Met－Ser, Val－Ser, Lys－Ala, Tyr－Ser 또는 Leu－Met에 대한 코오돈(codon)을 함유하고 2개의 뉴클레오 타이드 서열 사이의 판독 프레임(reading frame)에 위치하는 DNA 서열 뿐 아니라, DNA 서열 Ⅰ의 뉴클레오타이드 1 내지 약 1330 및 약 1340 내지 1442를 함유하는 방법.
  5. 제 1 내지 4항 중의 어느 한 항에 있어서, cDNA가 부가적으로 시그널펩타이드를 코오드화하는 cDNA인 방법.
  6. 제 1 내지 5항 중의 어느 한 항에 있어서, 프로테이나제 억제활성을 가지며, 제 1 도의 아미노산 서열Ⅰ중 1 내지 443번 및/또는 446 내지 480번의 아미노산을 함유하는 단백질을 제조하는 방법.
  7. 제 1 내지 6항 중의 어느 한 항에 있어서, 제 1 도의 아미노산 서열Ⅰ을 함유하는 단백질을 제조하는 방법.
  8. 아미노산 서열 Ⅰ에 상응하는 올리고 펩타이드로 면역화시켜 수득된 항체를 사용하는 친화성 크로마토그라피에 의해 조 단백질을 회수함을 특징으로 하여, 제 1 내지 7항 중의 어느 한 항에 따라 수득된 단백질 또는 당단백질을 분리하는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019860000867A 1985-02-08 1986-02-07 단백질 또는 당단백질의 제조방법 KR940003690B1 (ko)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DEP3504334.2 1985-02-08
DE3504334 1985-02-08
DEP3521226.8 1985-06-13
DE19853521226 DE3521226A1 (de) 1985-02-08 1985-06-13 Gene fuer biologisch aktive proteine

Publications (2)

Publication Number Publication Date
KR860006484A true KR860006484A (ko) 1986-09-11
KR940003690B1 KR940003690B1 (ko) 1994-04-27

Family

ID=25829228

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019860000867A KR940003690B1 (ko) 1985-02-08 1986-02-07 단백질 또는 당단백질의 제조방법

Country Status (11)

Country Link
US (1) US4849349A (ko)
EP (1) EP0190652B1 (ko)
KR (1) KR940003690B1 (ko)
DE (2) DE3521226A1 (ko)
DK (1) DK61486A (ko)
ES (2) ES8702493A1 (ko)
FI (1) FI84916C (ko)
GR (1) GR860360B (ko)
HU (1) HUT40167A (ko)
IE (1) IE58869B1 (ko)
PT (1) PT81978B (ko)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5422090A (en) * 1984-08-13 1995-06-06 Biotechnology Australia, Pty., Ltd. Human PAI-2
DE3709255A1 (de) * 1987-03-20 1988-09-29 Hoechst Ag Hybridserpine und dafuer kodierende dna
JPH01191687A (ja) * 1988-01-28 1989-08-01 Hoechst Japan Kk ヒトα↓２−プラスミンインヒビターまたはそれと類似の蛋白質から誘導される新規蛋白質
NL8902454A (nl) * 1989-10-03 1991-05-01 Stichting Centraal Lab Mutanten van de humane plasminogeen activator inhibitor 1 (pai-1), hun bereiding en toepassing, en recombinante polynucleotiden die voor deze mutanten coderende genetische informatie omvatten.
US5102995A (en) * 1989-10-20 1992-04-07 Washington University Dna encoding modified heparin cofactor ii
US5118793A (en) * 1989-10-20 1992-06-02 Washington University Modified heparin cofactor II
US5571783A (en) * 1993-03-09 1996-11-05 Clintec Nutrition Company Composition and method for treating patients with hepatic disease
DE19602345C2 (de) 1996-01-24 2002-12-12 Biotest Ag Verfahren zur Verbesserung des Wachstums und des kolorimetrischen Nachweises von Bakterien, Hefen, Pilzen oder Kokken

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2116183B (en) * 1982-03-03 1985-06-05 Genentech Inc Human antithrombin iii dna sequences therefore expression vehicles and cloning vectors containing such sequences and cell cultures transformed thereby a process for expressing human antithrombin iii and pharmaceutical compositions comprising it
BE895961A (fr) * 1983-02-21 1983-06-16 Wallone Region Procede de preparation d'un clone bacterien produisant de 1'alpha 1-antitrypsine humaine

Also Published As

Publication number Publication date
EP0190652A1 (de) 1986-08-13
GR860360B (en) 1986-06-03
ES8704544A1 (es) 1987-04-01
PT81978A (de) 1986-03-01
DK61486A (da) 1986-08-09
FI860540A0 (fi) 1986-02-06
DE3521226A1 (de) 1986-08-14
US4849349A (en) 1989-07-18
IE860350L (en) 1986-08-08
ES554102A0 (es) 1987-04-01
FI84916B (fi) 1991-10-31
ES8702493A1 (es) 1986-12-16
PT81978B (pt) 1988-07-01
IE58869B1 (en) 1993-11-17
EP0190652B1 (de) 1990-08-16
FI860540A (fi) 1986-08-09
HUT40167A (en) 1986-11-28
FI84916C (fi) 1992-02-10
KR940003690B1 (ko) 1994-04-27
DE3673436D1 (de) 1990-09-20
ES551695A0 (es) 1986-12-16
DK61486D0 (da) 1986-02-07

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