PT89367A - Melhoramentos na expressao e secrecao de proteinas heterologas em levedura utilizando sequencias guias alfa-factor truncadas - Google Patents
Melhoramentos na expressao e secrecao de proteinas heterologas em levedura utilizando sequencias guias alfa-factor truncadasInfo
- Publication number
- PT89367A PT89367A PT89367A PT8936788A PT89367A PT 89367 A PT89367 A PT 89367A PT 89367 A PT89367 A PT 89367A PT 8936788 A PT8936788 A PT 8936788A PT 89367 A PT89367 A PT 89367A
- Authority
- PT
- Portugal
- Prior art keywords
- yeast
- encodes
- precursor polypeptide
- precursor
- protein
- Prior art date
Links
- 240000004808 Saccharomyces cerevisiae Species 0.000 title abstract 5
- 108090000623 proteins and genes Proteins 0.000 title abstract 3
- 102000004169 proteins and genes Human genes 0.000 title abstract 2
- IXKSXJFAGXLQOQ-XISFHERQSA-N WHWLQLKPGQPMY Chemical compound C([C@@H](C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N1CCC[C@H]1C(=O)NCC(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(O)=O)NC(=O)[C@@H](N)CC=1C2=CC=CC=C2NC=1)C1=CNC=N1 IXKSXJFAGXLQOQ-XISFHERQSA-N 0.000 title 1
- 239000002243 precursor Substances 0.000 abstract 8
- 229920001184 polypeptide Polymers 0.000 abstract 7
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 7
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 7
- 108010058643 Fungal Proteins Proteins 0.000 abstract 5
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 abstract 4
- 108020004414 DNA Proteins 0.000 abstract 3
- 238000003776 cleavage reaction Methods 0.000 abstract 3
- 230000013595 glycosylation Effects 0.000 abstract 3
- 238000006206 glycosylation reaction Methods 0.000 abstract 3
- 230000007017 scission Effects 0.000 abstract 3
- 108091026890 Coding region Proteins 0.000 abstract 2
- 102000004877 Insulin Human genes 0.000 abstract 2
- 108090001061 Insulin Proteins 0.000 abstract 2
- 101150045458 KEX2 gene Proteins 0.000 abstract 2
- 238000001727 in vivo Methods 0.000 abstract 2
- 229940125396 insulin Drugs 0.000 abstract 2
- 102000053602 DNA Human genes 0.000 abstract 1
- 108010016626 Dipeptides Proteins 0.000 abstract 1
- 102000005593 Endopeptidases Human genes 0.000 abstract 1
- 108010059378 Endopeptidases Proteins 0.000 abstract 1
- 101000976075 Homo sapiens Insulin Proteins 0.000 abstract 1
- 108090000723 Insulin-Like Growth Factor I Proteins 0.000 abstract 1
- 102100037852 Insulin-like growth factor I Human genes 0.000 abstract 1
- 125000000729 N-terminal amino-acid group Chemical group 0.000 abstract 1
- 108010076181 Proinsulin Proteins 0.000 abstract 1
- 108010076504 Protein Sorting Signals Proteins 0.000 abstract 1
- 241000235070 Saccharomyces Species 0.000 abstract 1
- 150000001413 amino acids Chemical class 0.000 abstract 1
- PBGKTOXHQIOBKM-FHFVDXKLSA-N insulin (human) Chemical compound C([C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@H]1CSSC[C@H]2C(=O)N[C@H](C(=O)N[C@@H](CO)C(=O)N[C@H](C(=O)N[C@H](C(N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC=3C=CC(O)=CC=3)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC=3C=CC(O)=CC=3)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=3C=CC(O)=CC=3)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=3NC=NC=3)NC(=O)[C@H](CO)NC(=O)CNC1=O)C(=O)NCC(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)NCC(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)O)C(O)=O)C(=O)N[C@@H](CC(N)=O)C(O)=O)=O)CSSC[C@@H](C(N2)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@@H](NC(=O)CN)[C@@H](C)CC)[C@@H](C)CC)[C@@H](C)O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@@H](NC(=O)[C@@H](N)CC=1C=CC=CC=1)C(C)C)C1=CN=CN1 PBGKTOXHQIOBKM-FHFVDXKLSA-N 0.000 abstract 1
- 230000028327 secretion Effects 0.000 abstract 1
- 230000005026 transcription initiation Effects 0.000 abstract 1
- 230000005030 transcription termination Effects 0.000 abstract 1
- 210000005253 yeast cell Anatomy 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
- C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Diabetes (AREA)
- General Engineering & Computer Science (AREA)
- Endocrinology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Toxicology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US13968287A | 1987-12-30 | 1987-12-30 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| PT89367A true PT89367A (pt) | 1989-12-29 |
| PT89367B PT89367B (pt) | 1993-09-30 |
Family
ID=22487825
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PT89367A PT89367B (pt) | 1987-12-30 | 1988-12-30 | Melhoramentos na expressao e secrecao de proteinas heterologas em levedura utilizando sequencias guias alfa-factor truncadas |
Country Status (15)
| Country | Link |
|---|---|
| US (2) | US5602034A (pt) |
| EP (1) | EP0324274B1 (pt) |
| JP (1) | JP2793215B2 (pt) |
| AT (1) | ATE97441T1 (pt) |
| AU (1) | AU616228B2 (pt) |
| CA (1) | CA1340772C (pt) |
| DE (1) | DE3885728T2 (pt) |
| DK (2) | DK175910B1 (pt) |
| ES (1) | ES2059547T3 (pt) |
| FI (1) | FI100995B (pt) |
| HK (1) | HK139994A (pt) |
| IE (1) | IE62087B1 (pt) |
| IL (1) | IL88802A0 (pt) |
| NO (1) | NO178829C (pt) |
| PT (1) | PT89367B (pt) |
Families Citing this family (102)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5170964A (ja) * | 1974-12-16 | 1976-06-19 | Hohnen Oil | Haisuinoshoriho |
| EP0561137B1 (en) | 1983-04-25 | 2002-07-03 | Chiron Corporation | Hybrid DNA Synthesis of Mature Insulin-like Growth Factors |
| US5010182A (en) * | 1987-07-28 | 1991-04-23 | Chiron Corporation | DNA constructs containing a Kluyveromyces alpha factor leader sequence for directing secretion of heterologous polypeptides |
| WO1992004363A1 (en) * | 1990-09-04 | 1992-03-19 | The Salk Institute Biotechnology/Industrial Associates, Inc. | Production of insulin-like growth factor-1 in methylotrophic yeast cells |
| US5612198A (en) * | 1990-09-04 | 1997-03-18 | The Salk Institute | Production of insulin-like growth factor-1 in methylotrophic yeast cells |
| ATE177472T1 (de) * | 1990-09-14 | 1999-03-15 | Chiron Corp | Expression von makrophagen-induzierbaren proteinen (mips) in hefezellen |
| EP0574402B2 (en) | 1990-11-26 | 2012-08-01 | Genetics Institute, LLC | Expression of pace in host cells and methods of use thereof |
| DK300090D0 (da) * | 1990-12-19 | 1990-12-19 | Novo Nordisk As | Fremgangsmaade til fremstilling af leadersekvenser |
| DE69232666T2 (de) * | 1991-04-01 | 2003-03-20 | Merck & Co., Inc. | Gene, die die proteolytische aktivitaet von pichia beeinflussen und deren verwendung |
| US6348327B1 (en) | 1991-12-06 | 2002-02-19 | Genentech, Inc. | Non-endocrine animal host cells capable of expressing variant proinsulin and processing the same to form active, mature insulin and methods of culturing such cells |
| EP0682710B1 (en) * | 1993-02-10 | 2003-10-29 | Unilever Plc | Isolation process using immobilized proteins with specific binding capacities |
| US5538863A (en) * | 1993-07-01 | 1996-07-23 | Immunex Corporation | Expression system comprising mutant yeast strain and expression vector encoding synthetic signal peptide |
| US6451562B1 (en) | 1993-12-22 | 2002-09-17 | Human Genome Sciences, Inc. | Polypeptides encoding myeloid progenitor inhibitory factor-1 (MPIF-1) polynucleotides |
| US6495129B1 (en) | 1994-03-08 | 2002-12-17 | Human Genome Sciences, Inc. | Methods of inhibiting hematopoietic stem cells using human myeloid progenitor inhibitory factor-1 (MPIF-1) (Ckbeta-8/MIP-3) |
| US5629167A (en) | 1994-04-19 | 1997-05-13 | Biocine S.P.A. | Detection of antibodies against Chlamydia trachomatis pgp3 antigen in patient sera by enzyme-linked immunosorbent assay |
| RU2167939C2 (ru) * | 1994-06-16 | 2001-05-27 | Ново Нордиск А/С | Способ продуцирования полипептида в дрожжах |
| US5639642A (en) * | 1994-06-16 | 1997-06-17 | Novo Nordisk A/S | Synthetic leader peptide sequences |
| US6500645B1 (en) | 1994-06-17 | 2002-12-31 | Novo Nordisk A/S | N-terminally extended proteins expressed in yeast |
| IL114160A (en) * | 1994-06-17 | 2006-12-31 | Novo Nordisk As | Dna constructs encoding heterologous proteins and processes for the heterologous protein production in yeast |
| HRP940432B1 (en) * | 1994-08-05 | 2003-10-31 | Pliva Pharm & Chem Works | Dna sequences encoding biosynthetic insulin precursors and process for preparation of insulin |
| EP0779927A1 (en) * | 1994-09-08 | 1997-06-25 | Chiron Corporation | A method of improved production of insulin-like growth factor |
| GB9513967D0 (en) * | 1995-07-08 | 1995-09-06 | Univ Leicester | Insulin |
| ZA9610456B (en) * | 1995-12-20 | 1997-06-20 | Novo Nordisk As | N-terminally extended proteins expressed in yeast |
| CA2266543C (en) * | 1996-09-24 | 2003-12-09 | Cadus Pharmaceutical Corporation | Methods and compositions for identifying receptor effectors |
| US6197545B1 (en) | 1996-09-25 | 2001-03-06 | Vanderbilt University | Genetically engineered yeast with modified signal peptidase complex |
| JP4341859B2 (ja) | 1996-12-13 | 2009-10-14 | ノバルティス バクシンズ アンド ダイアグノスティックス， インコーポレーテッド | 酵母での異種タンパク質の発現の方法 |
| KR100381494B1 (ko) * | 1997-06-30 | 2003-07-22 | 바이오 테크놀로지 제네랄 코오포레이션 | 인간인슐린의제조방법 |
| US6068993A (en) * | 1997-07-02 | 2000-05-30 | Biobras Sa | Vector for expression of heterologous protein and methods for extracting recombinant protein and for purifying isolated recombinant insulin |
| EP1003889A1 (en) | 1997-08-05 | 2000-05-31 | Chiron Corporation | NOVEL $i(PICHIA PASTORIS) GENE SEQUENCES AND METHODS FOR THEIR USE |
| CA2671261A1 (en) | 1997-11-06 | 1999-05-20 | Novartis Vaccines And Diagnostics S.R.L. | Neisserial antigens |
| ES2333071T5 (es) | 1998-01-14 | 2015-08-17 | Novartis Vaccines And Diagnostics S.R.L. | Antígenos de Neisseria meningitidis |
| WO1999037793A1 (en) | 1998-01-23 | 1999-07-29 | Novo Nordisk A/S | Process for making desired polypeptides in yeast |
| GB9808932D0 (en) | 1998-04-27 | 1998-06-24 | Chiron Spa | Polyepitope carrier protein |
| EP2261338A3 (en) | 1998-05-01 | 2012-01-04 | Novartis Vaccines and Diagnostics, Inc. | Neisseria meningitidis antigens and compositions |
| WO2000005389A2 (en) * | 1998-07-20 | 2000-02-03 | Unilever N.V. | Production of proteins |
| EP1105485B1 (en) | 1998-08-21 | 2006-07-05 | Immunex Corporation | Human il-1 epsilon dna and polypeptides |
| WO2000066741A2 (en) | 1999-04-30 | 2000-11-09 | Chiron S.P.A. | Conserved neisserial antigens |
| GB9911683D0 (en) | 1999-05-19 | 1999-07-21 | Chiron Spa | Antigenic peptides |
| GB9916529D0 (en) | 1999-07-14 | 1999-09-15 | Chiron Spa | Antigenic peptides |
| MXPA02004283A (es) | 1999-10-29 | 2002-10-17 | Chiron Spa | Peptidos antigenicos neisseriales. |
| NZ520466A (en) | 2000-01-17 | 2003-09-26 | Chiron S | Outer membrane vesicle (OMV) vaccine comprising n. meningitidis serogroup B outer membrane proteins |
| DK1265630T3 (da) | 2000-03-24 | 2006-10-09 | Genentech Inc | Anvendelse af insulin til behandling af brusksygdomme |
| AU2002210126A1 (en) | 2000-05-25 | 2001-12-03 | Chiron Corporation | Iron regulation of the ppsec10 transcriptional regulatory region of pichia pastoris and methods of use |
| DE10085481T1 (de) * | 2000-07-26 | 2003-07-31 | Korea Res Inst Of Bioscience | Neue Zellwand-Anker-Proteine, die aus Hefe stammen, ihre Gene und Zelloberflächen-Expressionssysteme, in denen sie verwendet werden |
| CA2425303A1 (en) | 2000-10-27 | 2002-05-02 | John Telford | Nucleic acids and proteins from streptococcus groups a & b |
| GB0107658D0 (en) | 2001-03-27 | 2001-05-16 | Chiron Spa | Streptococcus pneumoniae |
| GB0107661D0 (en) | 2001-03-27 | 2001-05-16 | Chiron Spa | Staphylococcus aureus |
| CA2452767C (en) * | 2001-07-06 | 2013-03-19 | Juridical Foundation The Chemo-Sero-Therapeutic Research Institute | Genetic recombinant ecarin and process for preparing the same |
| ES2386386T3 (es) | 2001-12-12 | 2012-08-20 | Novartis Vaccines And Diagnostics S.R.L. | Inmunización contra Chlamydia trachomatis |
| ATE452137T1 (de) * | 2002-05-24 | 2010-01-15 | Restoragen Inc | Verfahren und konstrukte zur expression von clostripain mit hoher ausbeute |
| AU2003243316A1 (en) * | 2002-05-24 | 2003-12-12 | Nps Allelix Corp. | Method for enzymatic production of glp-2(1-33) and glp-2-(1-34) peptides |
| ATE492631T1 (de) | 2002-09-13 | 2011-01-15 | Cornell Res Foundation Inc | Verwendung von mutationen zur verbesserung von aspergillus-phytasen |
| US20070059329A1 (en) | 2002-11-15 | 2007-03-15 | Nathalie Norais | Unexpected surface proteins in meningococcus |
| GB0308198D0 (en) | 2003-04-09 | 2003-05-14 | Chiron Srl | ADP-ribosylating bacterial toxin |
| WO2005012530A2 (en) | 2003-07-25 | 2005-02-10 | Amgen Inc. | Antagonists and agonists of ldcam and methods of use |
| SI1704234T1 (sl) * | 2003-11-21 | 2012-08-31 | Nps Pharma Inc | Proizvodnja glukagonu podobnih peptidov 2 in analogov |
| JP5236189B2 (ja) | 2003-12-05 | 2013-07-17 | チルドレンズ ホスピタル メディカル センター | オリゴ糖組成物および感染症の治療における該組成物の使用 |
| JP2007519422A (ja) * | 2004-02-02 | 2007-07-19 | アンブレツクス・インコーポレイテツド | 修飾されたヒト四螺旋バンドルポリペプチド及びそれらの使用 |
| BRPI0512235A (pt) | 2004-06-18 | 2008-02-19 | Ambrx Inc | polipeptìdeos ligadores de antìgenos e seus usos |
| CA2569381A1 (en) * | 2004-07-21 | 2006-08-31 | Ambrx, Inc. | Biosynthetic polypeptides utilizing non-naturally encoded amino acids |
| WO2009100255A2 (en) | 2008-02-08 | 2009-08-13 | Ambrx, Inc. | Modified leptin polypeptides and their uses |
| JP2008525032A (ja) | 2004-12-22 | 2008-07-17 | アンブレツクス・インコーポレイテツド | 組換えヒト成長ホルモンを発現及び精製するための方法 |
| CN102732588B (zh) * | 2004-12-22 | 2015-01-07 | Ambrx公司 | 氨酰基-tRNA合成酶的组合物及其用途 |
| US7332571B2 (en) | 2004-12-22 | 2008-02-19 | Ambrx, Inc. | Compositions containing, methods involving, and uses of non-natural amino acids and polypeptides |
| EP1833993A4 (en) * | 2004-12-22 | 2009-07-22 | Ambrx Inc | HUMAN GROWTH HORMONE FORMULATIONS COMPRISING AN AMINO ACID CODED NON-NATURALLY |
| SG160437A1 (en) | 2004-12-22 | 2010-04-29 | Ambrx Inc | Modified human growth hormone |
| WO2006132925A2 (en) | 2005-06-01 | 2006-12-14 | University Of Pittsburgh Of The Commonwealth System Of Higher Education | Method of amidated peptide biosynthesis and delivery in vivo: endomorphin-2 for pain therapy |
| AU2006255122B2 (en) * | 2005-06-03 | 2010-10-21 | Ambrx, Inc. | Improved human interferon molecules and their uses |
| ES2408581T3 (es) | 2005-08-18 | 2013-06-21 | Ambrx, Inc. | Composiciones de ARNt y usos de las mismas |
| SI1954710T1 (sl) * | 2005-11-08 | 2011-08-31 | Ambrx Inc | Pospeĺ evala za modifikacijo nenaravnih aminokislin in nenaravnih peptidov aminokislin |
| WO2007059312A2 (en) | 2005-11-16 | 2007-05-24 | Ambrx, Inc. | Methods and compositions comprising non-natural amino acids |
| JP2009519942A (ja) * | 2005-12-14 | 2009-05-21 | アンブルックス，インコーポレイテッド | 非天然アミノ酸およびポリペプチドを含んでいる組成物、それらに関する方法、ならびに、それらの使用 |
| US20100015168A1 (en) | 2006-06-09 | 2010-01-21 | Novartis Ag | Immunogenic compositions for streptococcus agalactiae |
| JP5399906B2 (ja) * | 2006-09-08 | 2014-01-29 | アンブルックス，インコーポレイテッド | 脊椎動物細胞用のハイブリッドサプレッサーｔｒｎａ |
| MX2009002523A (es) | 2006-09-08 | 2009-03-20 | Ambrx Inc | Polipeptido de plasma humano modificado o andamios fc y sus usos. |
| WO2008030614A2 (en) * | 2006-09-08 | 2008-03-13 | Ambrx, Inc. | Suppressor trna transcription in vertebrate cells |
| JPWO2008032659A1 (ja) * | 2006-09-14 | 2010-01-21 | 独立行政法人産業技術総合研究所 | 効率向上型分泌シグナルペプチド及びそれらを利用したタンパク質生産方法 |
| EP2068909B1 (en) | 2007-03-30 | 2012-04-25 | Ambrx, Inc. | Modified fgf-21 polypeptides and their uses |
| JP2010525821A (ja) | 2007-05-02 | 2010-07-29 | アンブルックス，インコーポレイテッド | 修飾ＩＦＮβポリペプチドおよびこれらの使用 |
| EP2930182A1 (en) | 2007-11-20 | 2015-10-14 | Ambrx, Inc. | Modified insulin polypeptides and their uses |
| ES2963062T3 (es) | 2008-07-23 | 2024-03-25 | Ambrx Inc | Polipéptidos G-CSF bovinos modificados y sus usos |
| NZ592250A (en) | 2008-09-26 | 2012-11-30 | Lilly Co Eli | Modified animal erythropoietin polypeptides and their uses |
| NZ592249A (en) | 2008-09-26 | 2013-03-28 | Ambrx Inc | Non-natural amino acid replication-dependent microorganisms and vaccines |
| EP2258855A1 (en) | 2009-05-28 | 2010-12-08 | Universität für Bodenkultur Wien | Expression sequences |
| US20120213728A1 (en) * | 2009-10-30 | 2012-08-23 | Merck | Granulocyte-colony stimulating factor produced in glycoengineered pichia pastoris |
| BR112012015597A2 (pt) | 2009-12-21 | 2017-01-31 | Ambrx Inc | peptídeos de somatotropina suínos modificados e seus usos |
| NZ600361A (en) | 2009-12-21 | 2014-06-27 | Ambrx Inc | Modified bovine somatotropin polypeptides and their uses |
| SI2605789T1 (sl) | 2010-08-17 | 2019-10-30 | Ambrx Inc | Modificirani polipeptidi relaksina in njihova uporaba |
| US9567386B2 (en) | 2010-08-17 | 2017-02-14 | Ambrx, Inc. | Therapeutic uses of modified relaxin polypeptides |
| TWI480288B (zh) | 2010-09-23 | 2015-04-11 | Lilly Co Eli | 牛顆粒細胞群落刺激因子及其變體之調配物 |
| US20140234330A1 (en) | 2011-07-22 | 2014-08-21 | Amgen Inc. | Il-17 receptor a is required for il-17c biology |
| CA3212565A1 (en) | 2012-10-29 | 2014-05-08 | Lonza Ltd. | Leader peptides for expressing a protein of interest in an eukaryotic host cell |
| MX362515B (es) | 2013-04-30 | 2019-01-22 | Donald Danforth Plant Science Center | Proteinas péptidos y métodos de uso de las plantas antifúngicas. |
| JP6275847B2 (ja) | 2014-02-28 | 2018-02-07 | ノヴォ ノルディスク アー／エス | アルファ接合因子プロペプチドバリアント |
| LT3412302T (lt) | 2014-10-24 | 2021-07-26 | Bristol-Myers Squibb Company | Modifikuoti fgf-21 polipeptidai ir jų panaudojimai |
| PE20191716A1 (es) | 2017-02-08 | 2019-12-05 | Bristol Myers Squibb Co | Polipeptidos de relaxina modificada que comprenden un mejorador farmacocinetico y sus usos |
| WO2019154311A1 (zh) * | 2018-02-09 | 2019-08-15 | 江苏恒瑞医药股份有限公司 | 一种密码子优化的人胰岛素类似物前体基因和信号肽基因 |
| EP3884027A1 (en) | 2018-11-19 | 2021-09-29 | Basf Se | Leader sequence for yeast |
| CN110028562B (zh) * | 2019-04-25 | 2023-03-03 | 福州量衍科技有限公司 | 优化的Komagataella pastoris酵母α-factor信号肽及应用 |
| US12331302B2 (en) | 2019-11-08 | 2025-06-17 | The Regents Of The University Of California | Fungal autoinducible expression system |
| BR102019026867A2 (pt) * | 2019-12-16 | 2021-06-29 | Petróleo Brasileiro S.A. - Petrobras | Microrganismos geneticamente modificados que realizam a produção heteróloga de versões modificadas da proteína surfactante lv-ranaspumina-1 (lv-rsn-1), as versões modificadas dessa proteína surfactante, os genes sintéticos que codificam essa proteína surfactante, os cassetes de expressão contendo esses genes sintéticos, e os vetores de expressão contendo esses genes sintéticos |
| WO2026009000A1 (en) | 2024-07-04 | 2026-01-08 | Solasta Bio Limited | Methods for production of insecticidal neuropeptides |
Family Cites Families (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4366246A (en) | 1977-11-08 | 1982-12-28 | Genentech, Inc. | Method for microbial polypeptide expression |
| IL63270A0 (en) | 1980-08-05 | 1981-10-30 | Univ Leland Stanford Junior | Eukaryotic autonomously replicating segment |
| US4775622A (en) * | 1982-03-08 | 1988-10-04 | Genentech, Inc. | Expression, processing and secretion of heterologous protein by yeast |
| US4546082A (en) * | 1982-06-17 | 1985-10-08 | Regents Of The Univ. Of California | E. coli/Saccharomyces cerevisiae plasmid cloning vector containing the alpha-factor gene for secretion and processing of hybrid proteins |
| EP0116201B1 (en) | 1983-01-12 | 1992-04-22 | Chiron Corporation | Secretory expression in eukaryotes |
| CA1341302C (en) | 1983-02-22 | 2001-10-09 | Rae Lyn Burke | Yeast expression systems with vectors having gapdh or pyk promoters and synthesis of foreign protein |
| JPS60501140A (ja) * | 1983-04-22 | 1985-07-25 | アムジエン | 酵母による外因性ポリペプチドの分泌 |
| US4546083A (en) | 1983-04-22 | 1985-10-08 | Stolle Research & Development Corporation | Method and device for cell culture growth |
| NZ207926A (en) * | 1983-04-25 | 1988-04-29 | Genentech Inc | Use of yeast #a#-factor to assist in expression of proteins heterologus to yeast |
| EP0561137B1 (en) * | 1983-04-25 | 2002-07-03 | Chiron Corporation | Hybrid DNA Synthesis of Mature Insulin-like Growth Factors |
| US4870008A (en) * | 1983-08-12 | 1989-09-26 | Chiron Corporation | Secretory expression in eukaryotes |
| EP0164556B1 (en) | 1984-05-11 | 1994-03-02 | Chiron Corporation | Enhanced yeast transcription employing hybrid promoter region constructs |
| JPS6156078A (ja) * | 1984-07-27 | 1986-03-20 | Suntory Ltd | 酵母を宿主とする分泌発現ベクタ− |
| WO1986001515A1 (en) * | 1984-08-29 | 1986-03-13 | Chiron Corporation | Expression of human connective tissue activator and related peptides |
| DK129385A (da) * | 1985-03-22 | 1986-09-23 | Novo Industri As | Peptider og fremstilling deraf |
| JPS6236183A (ja) | 1985-06-20 | 1987-02-17 | ザ・サルク・インステイチユ−ト・バイオテクノロジ−／インダストリアル・アソシエイツ・インコ−ポレ−テツド | サツカロミセス・セレビシエからのポリペプチドの発現および分泌 |
| PH25772A (en) * | 1985-08-30 | 1991-10-18 | Novo Industri As | Insulin analogues, process for their preparation |
| US5010182A (en) | 1987-07-28 | 1991-04-23 | Chiron Corporation | DNA constructs containing a Kluyveromyces alpha factor leader sequence for directing secretion of heterologous polypeptides |
| JP3092811B2 (ja) * | 1988-07-23 | 2000-09-25 | デルタ バイオテクノロジー リミテッド | ペプチドおよびｄｎａ配列 |
-
1988
- 1988-12-22 CA CA000586916A patent/CA1340772C/en not_active Expired - Fee Related
- 1988-12-23 AT AT88312306T patent/ATE97441T1/de not_active IP Right Cessation
- 1988-12-23 DE DE88312306T patent/DE3885728T2/de not_active Expired - Lifetime
- 1988-12-23 EP EP88312306A patent/EP0324274B1/en not_active Expired - Lifetime
- 1988-12-23 ES ES88312306T patent/ES2059547T3/es not_active Expired - Lifetime
- 1988-12-26 IL IL88802A patent/IL88802A0/xx unknown
- 1988-12-28 FI FI886008A patent/FI100995B/fi not_active IP Right Cessation
- 1988-12-28 NO NO885786A patent/NO178829C/no unknown
- 1988-12-29 DK DK198807285A patent/DK175910B1/da not_active IP Right Cessation
- 1988-12-30 PT PT89367A patent/PT89367B/pt not_active IP Right Cessation
- 1988-12-30 JP JP63335685A patent/JP2793215B2/ja not_active Expired - Lifetime
- 1988-12-30 AU AU27650/88A patent/AU616228B2/en not_active Expired
- 1988-12-30 IE IE389688A patent/IE62087B1/en not_active IP Right Cessation
-
1994
- 1994-09-27 US US08/313,540 patent/US5602034A/en not_active Ceased
- 1994-12-08 HK HK139994A patent/HK139994A/en not_active IP Right Cessation
-
1998
- 1998-08-27 US US09/140,858 patent/USRE37343E1/en not_active Expired - Lifetime
-
2005
- 2005-01-27 DK DK200500130A patent/DK176075B1/da not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| NO178829C (no) | 1996-06-12 |
| AU616228B2 (en) | 1991-10-24 |
| IL88802A0 (en) | 1989-07-31 |
| DE3885728D1 (de) | 1993-12-23 |
| EP0324274A1 (en) | 1989-07-19 |
| NO885786D0 (no) | 1988-12-28 |
| DK728588A (da) | 1989-07-01 |
| CA1340772C (en) | 1999-09-28 |
| NO885786L (no) | 1989-07-03 |
| US5602034A (en) | 1997-02-11 |
| ATE97441T1 (de) | 1993-12-15 |
| HK139994A (en) | 1994-12-16 |
| IE62087B1 (en) | 1994-12-14 |
| EP0324274B1 (en) | 1993-11-18 |
| DK175910B1 (da) | 2005-06-13 |
| NO178829B (no) | 1996-03-04 |
| DK728588D0 (da) | 1988-12-29 |
| PT89367B (pt) | 1993-09-30 |
| DK200500130A (da) | 2005-01-27 |
| JP2793215B2 (ja) | 1998-09-03 |
| USRE37343E1 (en) | 2001-08-28 |
| FI100995B (fi) | 1998-03-31 |
| JPH022339A (ja) | 1990-01-08 |
| AU2765088A (en) | 1989-07-06 |
| DE3885728T2 (de) | 1994-03-10 |
| IE883896L (en) | 1989-06-30 |
| FI886008L (fi) | 1989-07-01 |
| ES2059547T3 (es) | 1994-11-16 |
| DK176075B1 (da) | 2006-04-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| PT89367A (pt) | Melhoramentos na expressao e secrecao de proteinas heterologas em levedura utilizando sequencias guias alfa-factor truncadas | |
| George-Nascimento et al. | Characterization of recombinant human epidermal growth factor produced in yeast | |
| NZ232742A (en) | Yeast heterologous protein production method, vectors, dna constructs and proteins so produced and aprotinin (1-58) so produced | |
| KR880014110A (ko) | 인체 프로아포지방단백질 a-i의 발현 | |
| DE3583824D1 (de) | Vorlaeufer des insulins, verfahren zu seiner herstellung und verfahren zur herstellung von humaninsulin aus solchen vorlaeufern des insulins. | |
| GB2008123A (en) | Method of means for microbial polypeptide expression | |
| KR890010198A (ko) | 효모에서 외래단백질을 제조하는 방법, 재조합 dna 및 형질전환체 | |
| KR910021477A (ko) | 인체 단백질 c의 글리코실화 돌연변이체의 발현을 위한 벡터 및 화합물 | |
| KR900700500A (ko) | 재조합 유전자 기술에 의한 에라스타제 억제 폴리펩티드 제조방법 및 에라스타제 억제 폴리펩티드 | |
| HUT52551A (en) | Process for production of neutrophil-activizating factor | |
| Heath et al. | (ACB) human proinsulin, a novel insulin agonist and intermediate in the synthesis of biosynthetic human insulin. | |
| AU7873798A (en) | N-terminally extended proteins expressed in yeast | |
| Lane et al. | The sequestration, processing and retention of honey‐bee promelittin made in amphibian oocytes | |
| EP0070632A2 (en) | Recombinant plasmids encoded for human preproinsulin and their use in producing human insulin | |
| NO971110L (no) | Fremgangsmåte for fremstilling av en modifisert, kollagenindusert blodplateaggregeringsinhibitor, pallidipin | |
| SK97195A3 (en) | Dnk - sequences coding the new biosynthetic precursors of insulin and method of insuline preparation | |
| KR880012758A (ko) | 폴리펩타이드의 유전공학적 제조방법 | |
| EP0232107A3 (en) | Human lymphotoxin polypeptide derivative | |
| KR860006484A (ko) | 단백질 또는 당단백질의 제조방법 | |
| CN101007848A (zh) | 长链人胰岛素样生长因子(lr3igf－1)及其制备和应用方法 | |
| KR890002395A (ko) | 인터루킨-2 활성을 갖는 폴리펩타이드 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG3A | Patent granted, date of granting |
Effective date: 19930317 |
|
| PD4A | Change of proprietorship |
Owner name: NOVARTIS VACCINES AND DIAGNOSTICS, INC., US Effective date: 20080215 |
|
| MM4A | Annulment/lapse due to non-payment of fees, searched and examined patent |
Free format text: MAXIMUM VALIDITY LIMIT REACHED Effective date: 20081230 |