AR070490A1 - Polipeptidos de thermomyces lanuginosus con actividad de lipasa y polinucleotidos que los codifican - Google Patents

Polipeptidos de thermomyces lanuginosus con actividad de lipasa y polinucleotidos que los codifican

Info

Publication number
AR070490A1
AR070490A1 ARP090100662A ARP090100662A AR070490A1 AR 070490 A1 AR070490 A1 AR 070490A1 AR P090100662 A ARP090100662 A AR P090100662A AR P090100662 A ARP090100662 A AR P090100662A AR 070490 A1 AR070490 A1 AR 070490A1
Authority
AR
Argentina
Prior art keywords
polypeptide
butyric acid
amount
polypeptides
lipase activity
Prior art date
Application number
ARP090100662A
Other languages
English (en)
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Publication of AR070490A1 publication Critical patent/AR070490A1/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)

Abstract

Polipéptidos obtenidos mediante la introduccion de mutaciones en una o más regiones identificadas en una lipasa original. Se ha encontrado que los polipéptidos tienen baja actividad específica hacia los ácidos grasos de cadena corta, lo que lleva a una reduccion de la generacion de olor y un BR incrementado con respecto a las lipasas conocidas en la técnica. Formulaciones para lavar la ropa. Reivindicacion 1: Un polipéptido con actividad de lipasa donde dicho polipéptido es un polipéptido que tiene por lo menos uno de los siguientes: a) una actividad de lipasa (LU) en relacion con la absorbancia a 280 nm (A280) de menos de 500 LU/A280, donde una unidad de LU (1 LU) se define como la cantidad de enzima con capacidad para liberar 1 micromol de ácido butírico por minuto a 30°C a pH 7, y la absorbancia del polipéptido se mide a 280 nm; b) un olor de eficiencia de riesgo (R) inferior a 0,5, donde R se calcula en términos de relacion entre la cantidad de ácido butírico liberado de una muestra de tela lavada con el polipéptido y la cantidad de ácido butírico liberada de una muestra lavada con el polipéptido de referencia, una vez corregidos ambos valores con respecto a la cantidad de ácido butírico liberada por una muestra lavada con un detergente no polipeptídico; o c) un factor Riesgo Beneficio (BR) de por lo menos 1,8, en el cual BR se define como la eficiencia de lavado promedio (RPprom) dividida por el olor de eficiencia de riesgo (R).
ARP090100662A 2008-02-29 2009-02-26 Polipeptidos de thermomyces lanuginosus con actividad de lipasa y polinucleotidos que los codifican AR070490A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US3244308P 2008-02-29 2008-02-29
EP08152163 2008-02-29

Publications (1)

Publication Number Publication Date
AR070490A1 true AR070490A1 (es) 2010-04-07

Family

ID=39745667

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP090100662A AR070490A1 (es) 2008-02-29 2009-02-26 Polipeptidos de thermomyces lanuginosus con actividad de lipasa y polinucleotidos que los codifican

Country Status (9)

Country Link
US (1) US7919298B2 (es)
EP (1) EP2250259B1 (es)
JP (1) JP5650543B2 (es)
CN (1) CN101960008B (es)
AR (1) AR070490A1 (es)
CA (1) CA2715829C (es)
ES (1) ES2603979T3 (es)
MX (1) MX2010009072A (es)
WO (1) WO2009109500A1 (es)

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