AR062336A1 - Recombinacion de homologos mediada por nucleasas en dedo de zinc - Google Patents

Recombinacion de homologos mediada por nucleasas en dedo de zinc

Info

Publication number
AR062336A1
AR062336A1 ARP070103561A ARP070103561A AR062336A1 AR 062336 A1 AR062336 A1 AR 062336A1 AR P070103561 A ARP070103561 A AR P070103561A AR P070103561 A ARP070103561 A AR P070103561A AR 062336 A1 AR062336 A1 AR 062336A1
Authority
AR
Argentina
Prior art keywords
sequence
sequences
vector
chromosomal dna
cell
Prior art date
Application number
ARP070103561A
Other languages
English (en)
Spanish (es)
Inventor
Lisa Lynn Schulenberg
Qihua C Cai
Jeffrey Miller
William Michael Ainley
Robbi Janette Garrison
Beth C Rubin-Wilson
Joseph F Petolino
Original Assignee
Dow Agrosciences Llc
Sangamo Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dow Agrosciences Llc, Sangamo Biosciences Inc filed Critical Dow Agrosciences Llc
Publication of AR062336A1 publication Critical patent/AR062336A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/14—Plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C07K2319/81—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor containing a Zn-finger domain for DNA binding

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
ARP070103561A 2006-08-11 2007-08-10 Recombinacion de homologos mediada por nucleasas en dedo de zinc AR062336A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US83714706P 2006-08-11 2006-08-11

Publications (1)

Publication Number Publication Date
AR062336A1 true AR062336A1 (es) 2008-10-29

Family

ID=38624400

Family Applications (3)

Application Number Title Priority Date Filing Date
ARP070103561A AR062336A1 (es) 2006-08-11 2007-08-10 Recombinacion de homologos mediada por nucleasas en dedo de zinc
ARP160102220A AR105435A2 (es) 2006-08-11 2016-07-21 Método de recombinación homóloga intramolecular en el genoma de una célula vegetal
ARP170100300A AR108684A2 (es) 2006-08-11 2017-02-06 Recombinación de homólogos mediada por nucleasas con dedos de zinc

Family Applications After (2)

Application Number Title Priority Date Filing Date
ARP160102220A AR105435A2 (es) 2006-08-11 2016-07-21 Método de recombinación homóloga intramolecular en el genoma de una célula vegetal
ARP170100300A AR108684A2 (es) 2006-08-11 2017-02-06 Recombinación de homólogos mediada por nucleasas con dedos de zinc

Country Status (19)

Country Link
US (2) US9428756B2 (enExample)
EP (2) EP2395081A1 (enExample)
JP (4) JP2010500029A (enExample)
KR (1) KR101439568B1 (enExample)
CN (2) CN101528924B (enExample)
AR (3) AR062336A1 (enExample)
AU (1) AU2007284748B2 (enExample)
BR (1) BRPI0716427A2 (enExample)
CA (1) CA2660485C (enExample)
DK (1) DK2049663T3 (enExample)
ES (1) ES2532002T3 (enExample)
HK (1) HK1221251A1 (enExample)
IL (1) IL196942A (enExample)
PL (1) PL2049663T3 (enExample)
SI (1) SI2049663T1 (enExample)
TW (1) TWI424061B (enExample)
UA (1) UA106193C2 (enExample)
WO (1) WO2008021207A2 (enExample)
ZA (1) ZA200900787B (enExample)

Families Citing this family (83)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2474486C (en) 2002-01-23 2013-05-14 The University Of Utah Research Foundation Targeted chromosomal mutagenesis using zinc finger nucleases
US7528643B2 (en) * 2003-02-12 2009-05-05 Semiconductor Energy Laboratory Co., Ltd. Semiconductor device, electronic device having the same, and driving method of the same
DK2412812T3 (en) * 2006-12-14 2015-05-26 Dow Agrosciences Llc Optimized non-canonical zinc finger proteins
EP2568048A1 (en) 2007-06-29 2013-03-13 Pioneer Hi-Bred International, Inc. Methods for altering the genome of a monocot plant cell
CA2700231C (en) * 2007-09-27 2018-09-18 Sangamo Biosciences, Inc. Rapid in vivo identification of biologically active nucleases
EP2602325B1 (en) 2008-02-29 2016-06-08 Monsanto Technology LLC Corn plant event MON87460 and compositions and methods for detection thereof
WO2009114321A2 (en) 2008-03-11 2009-09-17 Precision Biosciencs, Inc. Rationally-designed meganucleases for maize genome engineering
EP2281050B1 (en) * 2008-04-14 2014-04-02 Sangamo BioSciences, Inc. Linear donor constructs for targeted integration
EP2313515B1 (en) * 2008-08-22 2015-03-04 Sangamo BioSciences, Inc. Methods and compositions for targeted single-stranded cleavage and targeted integration
CN102369287B (zh) * 2009-04-07 2014-09-17 陶氏益农公司 纳米颗粒介导的序列特异性核酸酶的投递
US8598413B2 (en) 2009-08-19 2013-12-03 Dow AgroSciecnes, LLC. AAD-1 event DAS-40278-9, related transgenic corn lines, event-specific identification thereof, and methods of weed control involving AAD-1
EP2467010B1 (en) 2009-08-19 2018-11-28 Dow AgroSciences LLC Aad-1 event das-40278-9, related transgenic corn lines, and event-specific identification thereof
KR20120098681A (ko) * 2009-10-26 2012-09-05 독립행정법인농업생물자원연구소 유전적으로 개변된 식물세포의 제조방법
EP2319872A1 (en) 2009-11-04 2011-05-11 BASF Plant Science GmbH Amylopectin type starch with enhanced retrogradation stability
MX341974B (es) 2009-11-24 2016-09-07 Dow Agrosciences Llc Evento 416 de ariloaxialcanoato dioxigenasa, lineas de soja transgenica relacionadas y su identificacion especifica del evento.
GEP20176628B (en) * 2010-01-22 2017-02-27 Sangamo Biosciences Inc Targeted genomic alteration
JP2013526844A (ja) * 2010-03-22 2013-06-27 フィリップ・モーリス・プロダクツ・ソシエテ・アノニム 植物の酵素活性の改変
US8771985B2 (en) * 2010-04-26 2014-07-08 Sangamo Biosciences, Inc. Genome editing of a Rosa locus using zinc-finger nucleases
RU2608643C2 (ru) 2010-10-12 2017-01-23 Дзе Чилдрен`З Хоспитал Оф Филадельфия Способы и композиции для лечения гемофилии в
CA2820706C (en) 2010-12-03 2018-05-22 Ms Technologies, Llc Optimized expression of glyphosate resistance encoding nucleic acid molecules in plant cells
MX348731B (es) 2010-12-03 2017-06-27 Ms Tech Llc Caso de tolerancia a herbicida 8264.44.06.1 agrupado, líneas de frijol de soya transgénicas relacionadas y detección de las mismas.
CN102174649B (zh) * 2011-01-18 2013-06-12 陕西师范大学 快速检测锌指核酸酶介导基因定点整合的方法
BR112013025567B1 (pt) 2011-04-27 2021-09-21 Amyris, Inc Métodos para modificação genômica
ES2731638T3 (es) 2011-05-02 2019-11-18 Univ Nebraska Plantas con rasgos útiles y métodos relacionados
US9187567B2 (en) 2011-05-18 2015-11-17 Parkinson's Institute Assay to determine LRRK2 activity in parkinson's disease
EP2535416A1 (en) 2011-05-24 2012-12-19 BASF Plant Science Company GmbH Development of phytophthora resistant potato with increased yield
BR102012019434B1 (pt) 2011-07-26 2021-11-09 Dow Agrosciences Llc Métodos de controle de pestes, de insetos, molécula e sequência de dna diagnóstica para o evento de soja 9582.814.19.1
JP6185916B2 (ja) 2011-09-21 2017-08-23 サンガモ セラピューティクス， インコーポレイテッド 導入遺伝子発現を制御するための方法および組成物
EP2612918A1 (en) 2012-01-06 2013-07-10 BASF Plant Science Company GmbH In planta recombination
CN107043816A (zh) 2012-01-23 2017-08-15 陶氏益农公司 除草剂耐受性棉花事件pDAB4468.19.10.3
US9359614B2 (en) 2012-02-01 2016-06-07 Dow Agrosciences Llc Class of glyphosate resistance genes
US20150225734A1 (en) 2012-06-19 2015-08-13 Regents Of The University Of Minnesota Gene targeting in plants using dna viruses
US9914930B2 (en) 2012-09-07 2018-03-13 Dow Agrosciences Llc FAD3 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks
AR092482A1 (es) * 2012-09-07 2015-04-22 Dow Agrosciences Llc Enriquecimiento de la clasificacion de las celulas activadas por fluorescencia (facs) para generar plantas
UA119135C2 (uk) 2012-09-07 2019-05-10 ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі Спосіб отримання трансгенної рослини
UA118090C2 (uk) 2012-09-07 2018-11-26 ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі Спосіб інтегрування послідовності нуклеїнової кислоти, що представляє інтерес, у ген fad2 у клітині сої та специфічний для локусу fad2 білок, що зв'язується, здатний індукувати спрямований розрив
JP2016514480A (ja) 2013-04-05 2016-05-23 ダウ アグロサイエンシィズ エルエルシー 植物のゲノム内に外因性配列を組み込むための方法および組成物
AU2014279694B2 (en) 2013-06-14 2020-07-23 Cellectis Methods for non-transgenic genome editing in plants
US10767188B2 (en) 2013-09-25 2020-09-08 Nutech Ventures Methods and compositions for obtaining useful plant traits
UA120502C2 (uk) 2013-11-04 2019-12-26 Дау Агросайєнсиз Елелсі Спосіб отримання трансгенної рослини маїсу
TWI669395B (zh) * 2013-11-04 2019-08-21 美商陶氏農業科學公司 一種用於基因標定的通用供體系統
RU2019128647A (ru) 2013-11-04 2019-11-05 ДАУ АГРОСАЙЕНСИЗ ЭлЭлСи Оптимальные локусы кукурузы
NZ746567A (en) 2013-11-04 2019-09-27 Dow Agrosciences Llc Optimal soybean loci
EP3083958B1 (en) 2013-12-19 2019-04-17 Amyris, Inc. Methods for genomic integration
US10683513B2 (en) 2013-12-31 2020-06-16 Dow Agrosciences Llc Tissue-specific expression and hybrid plant production
ES2965286T3 (es) 2014-07-14 2024-04-12 Univ Washington State Silenciamiento de NANOS que abate las células germinales
US20190054117A1 (en) 2014-12-19 2019-02-21 Novartis Ag Dimerization switches and uses thereof
US20160281055A1 (en) 2015-03-20 2016-09-29 Dow Agrosciences Llc Incorporation of phosphatidylcholine in a media composition
CA2984013A1 (en) * 2015-05-12 2016-11-17 Sangamo Therapeutics, Inc. Nuclease-mediated regulation of gene expression
ES2981381T3 (es) 2015-08-06 2024-10-08 Univ Missouri Porcinos resistentes al virus del síndrome respiratorio y reproductivo porcino (PRRSV) y células que tienen genes CD163 modificados
WO2017132552A1 (en) 2016-01-27 2017-08-03 Oncorus, Inc. Oncolytic viral vectors and uses thereof
WO2017143071A1 (en) 2016-02-18 2017-08-24 The Regents Of The University Of California Methods and compositions for gene editing in stem cells
WO2017161043A1 (en) 2016-03-16 2017-09-21 The J. David Gladstone Institutes Methods and compositions for treating obesity and/or diabetes and for identifying candidate treatment agents
EP3458595A2 (en) 2016-05-18 2019-03-27 Amyris, Inc. Compositions and methods for genomic integration of nucleic acids into exogenous landing pads
US11078481B1 (en) 2016-08-03 2021-08-03 KSQ Therapeutics, Inc. Methods for screening for cancer targets
MY193234A (en) 2016-08-24 2022-09-27 Sangamo Therapeutics Inc Regulation of gene expression using engineered nucleases
KR20240144493A (ko) * 2016-08-24 2024-10-02 상가모 테라퓨틱스, 인코포레이티드 가공된 표적 특이적 뉴클레아제
US11078483B1 (en) 2016-09-02 2021-08-03 KSQ Therapeutics, Inc. Methods for measuring and improving CRISPR reagent function
KR20210035022A (ko) 2016-12-14 2021-03-31 리간달 인코포레이티드 핵산 및/또는 단백질 적재물 전달을 위한 조성물 및 방법
US11730828B2 (en) 2017-02-07 2023-08-22 The Regents Of The University Of California Gene therapy for haploinsufficiency
SG11201912179SA (en) 2017-06-15 2020-01-30 Univ California Targeted non-viral dna insertions
MX2020000993A (es) 2017-07-26 2020-07-22 Oncorus Inc Vectores virales oncolíticos y usos de estos.
IL305485B2 (en) 2017-10-27 2025-10-01 Univ California Targeted replacement of endogenous T cell receptors
EP4534678A3 (en) 2017-12-18 2025-06-18 Syngenta Crop Protection AG Targeted insertion sites in the maize genome
SG11202103314QA (en) * 2018-10-02 2021-04-29 Sangamo Therapeutics Inc Engineered genetic modulators
CA3115156A1 (en) 2018-10-02 2020-04-09 Sangamo Therapeutics, Inc. Methods and compositions for modulation of tau proteins
US20200157531A1 (en) * 2018-11-16 2020-05-21 Wisconsin Alumni Research Foundation Method to enhance screening for homologous recombination in genome edited cells using recombination-activated fluorescent donor delivery vector
WO2020163856A1 (en) 2019-02-10 2020-08-13 The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Gladstone Modified mitochondrion and methods of use thereof
US12509703B2 (en) 2019-05-21 2025-12-30 Sangamo Therapeutics, Inc. Controlled transgene expression in regulatory t cells
EP3999627A2 (en) 2019-07-18 2022-05-25 University of Rochester Cell-type selective immunoprotection of cells
WO2021028359A1 (en) 2019-08-09 2021-02-18 Sangamo Therapeutics France Controlled expression of chimeric antigen receptors in t cells
CN111462823B (zh) * 2020-04-08 2022-07-12 西安交通大学 一种基于dna测序数据的同源重组缺陷判定方法
US20240000051A1 (en) 2020-11-16 2024-01-04 Pig Improvement Company Uk Limited Influenza a-resistant animals having edited anp32 genes
KR20230155448A (ko) 2021-02-10 2023-11-10 온코루스, 인크. 화합물, 조성물 및 이의 사용 방법
MX2024003887A (es) 2021-10-14 2024-07-09 Arsenal Biosciences Inc Células inmunitarias que tienen arnch coespresados y sistemas de compuerta lógica.
US20250043269A1 (en) 2021-11-03 2025-02-06 The J. David Gladstone Institutes, a testamentary trust established under the Will of J. David Glad Precise Genome Editing Using Retrons
WO2023105244A1 (en) 2021-12-10 2023-06-15 Pig Improvement Company Uk Limited Editing tmprss2/4 for disease resistance in livestock
WO2023141602A2 (en) 2022-01-21 2023-07-27 Renagade Therapeutics Management Inc. Engineered retrons and methods of use
WO2024013514A2 (en) 2022-07-15 2024-01-18 Pig Improvement Company Uk Limited Gene edited livestock animals having coronavirus resistance
WO2024044723A1 (en) 2022-08-25 2024-02-29 Renagade Therapeutics Management Inc. Engineered retrons and methods of use
AU2024246543A1 (en) 2023-03-31 2025-10-30 Briacell Therapeutics Corp. Methods for enhancing the immunogenicity of cellular vaccines
WO2025049959A2 (en) 2023-09-01 2025-03-06 Renagade Therapeutics Management Inc. Gene editing systems, compositions, and methods for treatment of vexas syndrome
WO2025174765A1 (en) 2024-02-12 2025-08-21 Renagade Therapeutics Management Inc. Lipid nanoparticles comprising coding rna molecules for use in gene editing and as vaccines and therapeutic agents

Family Cites Families (58)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0222493A1 (en) 1985-10-04 1987-05-20 Lubrizol Genetics Inc. TR-based sub-TI plasmids
US5422251A (en) 1986-11-26 1995-06-06 Princeton University Triple-stranded nucleic acids
ATE112314T1 (de) 1988-05-17 1994-10-15 Lubrizol Genetics Inc Pflanzliches ubiquitinpromotorsystem.
US5176996A (en) 1988-12-20 1993-01-05 Baylor College Of Medicine Method for making synthetic oligonucleotides which bind specifically to target sites on duplex DNA molecules, by forming a colinear triplex, the synthetic oligonucleotides and methods of use
FR2646438B1 (fr) 1989-03-20 2007-11-02 Pasteur Institut Procede de remplacement specifique d'une copie d'un gene present dans le genome receveur par l'integration d'un gene different de celui ou se fait l'integration
US5420032A (en) 1991-12-23 1995-05-30 Universitge Laval Homing endonuclease which originates from chlamydomonas eugametos and recognizes and cleaves a 15, 17 or 19 degenerate double stranded nucleotide sequence
US5436150A (en) 1992-04-03 1995-07-25 The Johns Hopkins University Functional domains in flavobacterium okeanokoities (foki) restriction endonuclease
US5487994A (en) 1992-04-03 1996-01-30 The Johns Hopkins University Insertion and deletion mutants of FokI restriction endonuclease
US5356802A (en) 1992-04-03 1994-10-18 The Johns Hopkins University Functional domains in flavobacterium okeanokoites (FokI) restriction endonuclease
US5792632A (en) 1992-05-05 1998-08-11 Institut Pasteur Nucleotide sequence encoding the enzyme I-SceI and the uses thereof
US5591616A (en) 1992-07-07 1997-01-07 Japan Tobacco, Inc. Method for transforming monocotyledons
US6242568B1 (en) 1994-01-18 2001-06-05 The Scripps Research Institute Zinc finger protein derivatives and methods therefor
AU704601B2 (en) 1994-01-18 1999-04-29 Scripps Research Institute, The Zinc finger protein derivatives and methods therefor
US6140466A (en) 1994-01-18 2000-10-31 The Scripps Research Institute Zinc finger protein derivatives and methods therefor
US5585245A (en) 1994-04-22 1996-12-17 California Institute Of Technology Ubiquitin-based split protein sensor
GB9824544D0 (en) 1998-11-09 1999-01-06 Medical Res Council Screening system
EP0781331B1 (en) 1994-08-20 2008-09-03 Gendaq Limited Improvements in or relating to binding proteins for recognition of dna
US5789538A (en) 1995-02-03 1998-08-04 Massachusetts Institute Of Technology Zinc finger proteins with high affinity new DNA binding specificities
US5773695A (en) 1996-01-26 1998-06-30 North Carolina State University Plant nuclear scaffold attachment region and method for increasing gene expression in transgenic cells
US5925523A (en) 1996-08-23 1999-07-20 President & Fellows Of Harvard College Intraction trap assay, reagents and uses thereof
GB9703369D0 (en) 1997-02-18 1997-04-09 Lindqvist Bjorn H Process
GB2338237B (en) 1997-02-18 2001-02-28 Actinova Ltd In vitro peptide or protein expression library
US6342345B1 (en) 1997-04-02 2002-01-29 The Board Of Trustees Of The Leland Stanford Junior University Detection of molecular interactions by reporter subunit complementation
GB9710807D0 (en) 1997-05-23 1997-07-23 Medical Res Council Nucleic acid binding proteins
GB9710809D0 (en) 1997-05-23 1997-07-23 Medical Res Council Nucleic acid binding proteins
DK0991764T3 (da) 1997-06-12 2006-11-13 Dow Agrosciences Llc Regulatoriske sekvenser for transgene planter
DK1034262T3 (da) * 1997-11-18 2005-11-28 Pioneer Hi Bred Int Sammensætninger og fremgangsmåder til genetisk modifikation af planter
US6506559B1 (en) 1997-12-23 2003-01-14 Carnegie Institute Of Washington Genetic inhibition by double-stranded RNA
AR014491A1 (es) 1998-01-29 2001-02-28 Dow Agrosciences Llc Metodo para obtener plantas transgenicas fertiles de gossypium hirsutum.
US6410248B1 (en) 1998-01-30 2002-06-25 Massachusetts Institute Of Technology General strategy for selecting high-affinity zinc finger proteins for diverse DNA target sites
AU746454B2 (en) 1998-03-02 2002-05-02 Massachusetts Institute Of Technology Poly zinc finger proteins with improved linkers
US6140081A (en) 1998-10-16 2000-10-31 The Scripps Research Institute Zinc finger binding domains for GNN
US6453242B1 (en) 1999-01-12 2002-09-17 Sangamo Biosciences, Inc. Selection of sites for targeting by zinc finger proteins and methods of designing zinc finger proteins to bind to preselected sites
US6534261B1 (en) 1999-01-12 2003-03-18 Sangamo Biosciences, Inc. Regulation of endogenous gene expression in cells using zinc finger proteins
US7030215B2 (en) 1999-03-24 2006-04-18 Sangamo Biosciences, Inc. Position dependent recognition of GNN nucleotide triplets by zinc fingers
US6794136B1 (en) 2000-11-20 2004-09-21 Sangamo Biosciences, Inc. Iterative optimization in the design of binding proteins
CA2394850C (en) 1999-12-06 2012-02-07 Sangamo Biosciences, Inc. Methods of using randomized libraries of zinc finger proteins for the identification of gene function
AU2693501A (en) 2000-01-24 2001-07-31 Gendaq Ltd Nucleic acid binding polypeptides characterized by flexible linkers connected nucleic acid binding modules
US20020061512A1 (en) 2000-02-18 2002-05-23 Kim Jin-Soo Zinc finger domains and methods of identifying same
CA2407460C (en) 2000-04-28 2013-06-25 Sangamo Biosciences, Inc. Targeted modification of chromatin structure
WO2001088197A2 (en) 2000-05-16 2001-11-22 Massachusetts Institute Of Technology Methods and compositions for interaction trap assays
JP2002060786A (ja) 2000-08-23 2002-02-26 Kao Corp 硬質表面用殺菌防汚剤
DK1353941T3 (da) 2001-01-22 2013-06-17 Sangamo Biosciences Inc Modificerede zinkfingerbindingsproteiner
GB0108491D0 (en) 2001-04-04 2001-05-23 Gendaq Ltd Engineering zinc fingers
WO2002080809A2 (en) 2001-04-05 2002-10-17 The Regents Of The University Of California Robotic device for locomotor training
JP2005500061A (ja) 2001-08-20 2005-01-06 ザ スクリップス リサーチ インスティテュート Ｃｎｎについての亜鉛フィンガー結合ドメイン
US7462758B2 (en) 2001-12-20 2008-12-09 Sungene Gmbh & Co. Kgaa Methods for the transformation of vegetal plastids
US7262054B2 (en) 2002-01-22 2007-08-28 Sangamo Biosciences, Inc. Zinc finger proteins for DNA binding and gene regulation in plants
CA2474486C (en) 2002-01-23 2013-05-14 The University Of Utah Research Foundation Targeted chromosomal mutagenesis using zinc finger nucleases
US20030232410A1 (en) 2002-03-21 2003-12-18 Monika Liljedahl Methods and compositions for using zinc finger endonucleases to enhance homologous recombination
US9447434B2 (en) 2002-09-05 2016-09-20 California Institute Of Technology Use of chimeric nucleases to stimulate gene targeting
US7888121B2 (en) 2003-08-08 2011-02-15 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
DK2927318T3 (da) 2003-08-08 2020-08-03 Sangamo Therapeutics Inc Fremgangsmåde og sammensætninger til målrettet spaltning og rekombination
US20050102713A1 (en) 2003-11-07 2005-05-12 Merlo Donald J. Use of untranslated region of osmotin gene to enhance transgene expression in plants
EP1727906B1 (en) 2004-03-17 2012-02-22 BASF Plant Science GmbH Improved constructs for marker excision based on dual-function selection marker
AU2005287547B2 (en) 2004-09-23 2010-09-02 Basf Plant Science Gmbh Recombination cassettes and methods for sequence excision in plants
SI1869186T1 (sl) 2005-04-04 2011-02-28 Bayer Bioscience Nv Postopki in sredstva za odstranitev izbranega DNA zaporedja
WO2007014275A2 (en) 2005-07-26 2007-02-01 Sangamo Biosciences, Inc. Targeted integration and expression of exogenous nucleic acid sequences

Also Published As

Publication number Publication date
CA2660485A1 (en) 2008-02-21
AR108684A2 (es) 2018-09-19
KR20090054986A (ko) 2009-06-01
IL196942A0 (en) 2011-08-01
JP2010500029A (ja) 2010-01-07
BRPI0716427A2 (pt) 2014-03-11
EP2395081A1 (en) 2011-12-14
WO2008021207A2 (en) 2008-02-21
CN101528924A (zh) 2009-09-09
AU2007284748B2 (en) 2013-05-16
CA2660485C (en) 2016-04-12
EP2049663B1 (en) 2015-02-25
ES2532002T3 (es) 2015-03-23
US10669551B2 (en) 2020-06-02
AR105435A2 (es) 2017-10-04
HK1134320A1 (zh) 2010-04-23
IL196942A (en) 2014-06-30
JP2014193179A (ja) 2014-10-09
AU2007284748A1 (en) 2008-02-21
JP6652952B2 (ja) 2020-02-26
ZA200900787B (en) 2010-05-26
CN101528924B (zh) 2015-11-25
CN105296527B (zh) 2020-11-27
JP2018029603A (ja) 2018-03-01
UA106193C2 (ru) 2014-08-11
PL2049663T3 (pl) 2015-08-31
US20160326537A1 (en) 2016-11-10
JP2016195606A (ja) 2016-11-24
US20100257638A1 (en) 2010-10-07
CN105296527A (zh) 2016-02-03
KR101439568B1 (ko) 2014-09-12
SI2049663T1 (sl) 2015-12-31
TWI424061B (zh) 2014-01-21
JP6442442B2 (ja) 2018-12-19
JP5964884B2 (ja) 2016-08-03
TW200815593A (en) 2008-04-01
DK2049663T3 (en) 2015-06-01
EP2049663A1 (en) 2009-04-22
US9428756B2 (en) 2016-08-30
HK1221251A1 (zh) 2017-05-26

Similar Documents

Publication Publication Date Title
AR062336A1 (es) Recombinacion de homologos mediada por nucleasas en dedo de zinc
MX2022008197A (es) Metodo para el tratamiento del sindrome de usher y composicion del mismo.
AR086995A1 (es) Metodos y composiciones para producir plantas con esterilidad masculina
JP2010500029A5 (enExample)
AR098300A1 (es) Locus óptimos de la soja
DK1546174T3 (da) Fremgangsmåder og sammensætninger til RNA-interferens
AR086031A2 (es) Un metodo para remover al menos una secuencia transgenica en una celula vegetal o planta
RU2016104056A (ru) Мультиплексная геномная инженерия, направляемая рнк
MX2020011939A (es) Nuevas enzimas y sistemas crispr.
AR093045A2 (es) Un metodo de aplicar un herbicida a una planta transgenica para producir una semilla hibrida no natural transgenica, celulas no naturales y adn recombinante
AR083432A1 (es) Precursor de microarn y metodos para regular la expresion del gen objetivo
AR039717A1 (es) Construcciones de arn de intrones cadena doble y usos de las mismas
EA201691581A1 (ru) Способы и композиции для повышения эффективности направленной модификации генов с применением опосредованной олигонуклеотидами репарации генов
WO2010080129A3 (en) Extended dicer substrate agents and methods for the specific inhibition of gene expression
RU2014127702A (ru) Модифицированные Cascade-рибонуклеопротеины и их применения
WO2006073601A3 (en) Methods and compositions for rna interference
AR098299A1 (es) Locus óptimos del maíz
WO2002059300A3 (en) Double-stranded rna-mediated gene suppression
AR059570A1 (es) Seleccion y estabilizacion de constructos de arn ds
AR069821A1 (es) Composiciones y metodos de uso de la interferencia de arn para el control de nematodos
ATE485375T1 (de) Nukleinsäuren zum auslösen der tumorzellsterblichkeit
AR101779A2 (es) Secuencias de nucleótidos msca1 que afectan la fertilidad vegetal masculina y método para usar las mismas
ES2641014T3 (es) Gen Dro1 que controla características de enraizamiento profundo de planta y utilización del mismo
AR074810A1 (es) Plantas y cromosomas de soja transgenica
AR081594A1 (es) Metodos y medios para modificar un genoma vegetal en una secuencia de nucleotidos usada comunmente en la ingenieria genetica de genomas vegetales

Legal Events

Date Code Title Description
FG Grant, registration