UA106193C2 - Гомологичная рекомбинация, опосредованная нуклеазами с цинковыми пальцами - Google Patents

Гомологичная рекомбинация, опосредованная нуклеазами с цинковыми пальцами

Info

Publication number
UA106193C2
UA106193C2 UAA200902103A UAA200902103A UA106193C2 UA 106193 C2 UA106193 C2 UA 106193C2 UA A200902103 A UAA200902103 A UA A200902103A UA A200902103 A UAA200902103 A UA A200902103A UA 106193 C2 UA106193 C2 UA 106193C2
Authority
UA
Ukraine
Prior art keywords
sequence
sequences
genome
plant cell
dna
Prior art date
Application number
UAA200902103A
Other languages
English (en)
Russian (ru)
Ukrainian (uk)
Inventor
Цихуа Цай
Джеффри Миллер
Уилльям Майкл Эйнли
Робби Дженетт Гаррисон
Джозеф Ф. Петолино
Бет Кори Рубин-Уилсон
Лиза Линн Шуленберг
Эндрю Фредерик Уорден
Original Assignee
ДАУ АГРОСАЙЕНСИЗ ЕлЕлСи
Сангамо Байосайенсиз, Инк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ДАУ АГРОСАЙЕНСИЗ ЕлЕлСи, Сангамо Байосайенсиз, Инк. filed Critical ДАУ АГРОСАЙЕНСИЗ ЕлЕлСи
Publication of UA106193C2 publication Critical patent/UA106193C2/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/14—Plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C07K2319/81—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor containing a Zn-finger domain for DNA binding

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
UAA200902103A 2006-08-11 2007-08-09 Гомологичная рекомбинация, опосредованная нуклеазами с цинковыми пальцами UA106193C2 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US83714706P 2006-08-11 2006-08-11
PCT/US2007/017748 WO2008021207A2 (en) 2006-08-11 2007-08-09 Zinc finger nuclease-mediated homologous recombination

Publications (1)

Publication Number Publication Date
UA106193C2 true UA106193C2 (ru) 2014-08-11

Family

ID=38624400

Family Applications (1)

Application Number Title Priority Date Filing Date
UAA200902103A UA106193C2 (ru) 2006-08-11 2007-08-09 Гомологичная рекомбинация, опосредованная нуклеазами с цинковыми пальцами

Country Status (19)

Country Link
US (2) US9428756B2 (enExample)
EP (2) EP2049663B1 (enExample)
JP (4) JP2010500029A (enExample)
KR (1) KR101439568B1 (enExample)
CN (2) CN101528924B (enExample)
AR (3) AR062336A1 (enExample)
AU (1) AU2007284748B2 (enExample)
BR (1) BRPI0716427A2 (enExample)
CA (1) CA2660485C (enExample)
DK (1) DK2049663T3 (enExample)
ES (1) ES2532002T3 (enExample)
HK (1) HK1221251A1 (enExample)
IL (1) IL196942A (enExample)
PL (1) PL2049663T3 (enExample)
SI (1) SI2049663T1 (enExample)
TW (1) TWI424061B (enExample)
UA (1) UA106193C2 (enExample)
WO (1) WO2008021207A2 (enExample)
ZA (1) ZA200900787B (enExample)

Families Citing this family (84)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE347593T1 (de) 2002-01-23 2006-12-15 Univ Utah Res Found Zielgerichtete chromosomale mutagenese mit zinkfingernukleasen
US7528643B2 (en) * 2003-02-12 2009-05-05 Semiconductor Energy Laboratory Co., Ltd. Semiconductor device, electronic device having the same, and driving method of the same
CA2669746C (en) * 2006-12-14 2017-05-16 Dow Agrosciences Llc Optimized non-canonical zinc finger proteins
CA2691440A1 (en) 2007-06-29 2009-01-08 Pioneer Hi-Bred International, Inc. Methods for altering the genome of a monocot plant cell
JP5763341B2 (ja) * 2007-09-27 2015-08-12 サンガモ バイオサイエンシーズ, インコーポレイテッド 生物学的活性のあるヌクレアーゼの迅速なインビボ同定法
EP2247736B1 (en) 2008-02-29 2013-05-15 Monsanto Technology, LLC Corn plant event mon87460 and compositions and methods for detection thereof
WO2009114321A2 (en) 2008-03-11 2009-09-17 Precision Biosciencs, Inc. Rationally-designed meganucleases for maize genome engineering
CA2720903C (en) * 2008-04-14 2019-01-15 Sangamo Biosciences, Inc. Linear donor constructs for targeted integration
JP5908725B2 (ja) * 2008-08-22 2016-04-26 サンガモ バイオサイエンシーズ, インコーポレイテッド 標的一本鎖開裂および標的組込みのための方法、並びに組成物
CA2757831C (en) * 2009-04-07 2017-09-26 Dow Agrosciences Llc Nanoparticle mediated delivery of sequence specific nucleases
US8598413B2 (en) 2009-08-19 2013-12-03 Dow AgroSciecnes, LLC. AAD-1 event DAS-40278-9, related transgenic corn lines, event-specific identification thereof, and methods of weed control involving AAD-1
CA2771538C (en) * 2009-08-19 2019-06-04 Dow Agrosciences Llc Aad-1 event das-40278-9, related transgenic corn lines, and event-specific identification thereof
EP2495313A4 (en) * 2009-10-26 2013-03-20 Nat Inst Of Agrobio Sciences METHOD FOR PRODUCING GENUANIPULATED PLANT CELLS
EP2319872A1 (en) 2009-11-04 2011-05-11 BASF Plant Science GmbH Amylopectin type starch with enhanced retrogradation stability
AU2010324752B2 (en) 2009-11-24 2016-04-21 Dow Agrosciences Llc AAD-12 event 416, related transgenic soybean lines, and event-specific identification thereof
CA2787494C (en) * 2010-01-22 2019-09-17 Dow Agrosciences Llc Targeted genomic alteration
US20130198897A1 (en) * 2010-03-22 2013-08-01 Philip Morris Products S.A. Modifying enzyme activity in plants
US9567573B2 (en) * 2010-04-26 2017-02-14 Sangamo Biosciences, Inc. Genome editing of a Rosa locus using nucleases
EP2627665B1 (en) 2010-10-12 2015-12-16 The Children's Hospital Of Philadelphia Methods and compositions for treating hemophilia B
CN103561564B (zh) 2010-12-03 2016-11-16 陶氏益农公司 叠加的除草剂耐受性事件8264.44.06.1、相关转基因大豆系、及其检测
CA2820706C (en) 2010-12-03 2018-05-22 Ms Technologies, Llc Optimized expression of glyphosate resistance encoding nucleic acid molecules in plant cells
CN102174649B (zh) * 2011-01-18 2013-06-12 陕西师范大学 快速检测锌指核酸酶介导基因定点整合的方法
CA2834375C (en) 2011-04-27 2020-07-14 Amyris, Inc. Methods for genomic modification
CA2834679C (en) 2011-05-02 2021-12-28 Board Of Regents Of The University Of Nebraska Method for producing a plant with useful traits by suppressing msh1 gene
EP2710041A4 (en) 2011-05-18 2014-11-05 Parkinson S Inst ASSAY FOR DETERMINING LRRK2 ACTIVITY IN MORBUS PARKINSON
EP2535416A1 (en) 2011-05-24 2012-12-19 BASF Plant Science Company GmbH Development of phytophthora resistant potato with increased yield
BR102012019434B1 (pt) 2011-07-26 2021-11-09 Dow Agrosciences Llc Métodos de controle de pestes, de insetos, molécula e sequência de dna diagnóstica para o evento de soja 9582.814.19.1
EP2758529B1 (en) 2011-09-21 2019-03-20 Sangamo Therapeutics, Inc. Methods and compositions for regulation of transgene expression
EP2612918A1 (en) 2012-01-06 2013-07-10 BASF Plant Science Company GmbH In planta recombination
US9353381B2 (en) 2012-01-23 2016-05-31 Dow Agrosciences Llc Cotton event pDAB4468.19.10.3 detection method
US9506077B2 (en) 2012-02-01 2016-11-29 Dow Agrosciences Llc Synthetic chloroplast transit peptides
US20150225734A1 (en) 2012-06-19 2015-08-13 Regents Of The University Of Minnesota Gene targeting in plants using dna viruses
BR112015004995B1 (pt) 2012-09-07 2023-05-02 Sangamo Biosciences, Inc. Método para modificação do genoma de uma célula, uso de uma célula, semente ou planta obtida pelo referido método e nuclease de dedo de zinco sítio específica
UA119135C2 (uk) 2012-09-07 2019-05-10 ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі Спосіб отримання трансгенної рослини
UA118090C2 (uk) 2012-09-07 2018-11-26 ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі Спосіб інтегрування послідовності нуклеїнової кислоти, що представляє інтерес, у ген fad2 у клітині сої та специфічний для локусу fad2 білок, що зв'язується, здатний індукувати спрямований розрив
US20140075593A1 (en) * 2012-09-07 2014-03-13 Dow Agrosciences Llc Fluorescence activated cell sorting (facs) enrichment to generate plants
CN105263312A (zh) 2013-04-05 2016-01-20 美国陶氏益农公司 用于在植物基因组内整合外源序列的方法和组合物
US20160145631A1 (en) 2013-06-14 2016-05-26 Cellectis Methods for non-transgenic genome editing in plants
US10767188B2 (en) 2013-09-25 2020-09-08 Nutech Ventures Methods and compositions for obtaining useful plant traits
JP6633532B2 (ja) 2013-11-04 2020-01-22 ダウ アグロサイエンシィズ エルエルシー 最適なトウモロコシ遺伝子座
EP3066110B1 (en) 2013-11-04 2021-12-29 Corteva Agriscience LLC Optimal maize loci
BR102014027448A8 (pt) * 2013-11-04 2021-08-24 Dow Agrosciences Llc cassete de doador de polinucleotídeo, uso de uma planta, de uma parte de planta, ou de uma célula vegetal compreendendo o referido cassete, e método para integração direcionada do mesmo
WO2015066643A1 (en) 2013-11-04 2015-05-07 Dow Agrosciences Llc Optimal soybean loci
US9476065B2 (en) 2013-12-19 2016-10-25 Amyris, Inc. Methods for genomic integration
US10683513B2 (en) 2013-12-31 2020-06-16 Dow Agrosciences Llc Tissue-specific expression and hybrid plant production
EP3169778B1 (en) 2014-07-14 2023-10-25 Washington State University Nanos knock-out that ablates germline cells
WO2016098078A2 (en) 2014-12-19 2016-06-23 Novartis Ag Dimerization switches and uses thereof
US20160281055A1 (en) 2015-03-20 2016-09-29 Dow Agrosciences Llc Incorporation of phosphatidylcholine in a media composition
SG10202112057QA (en) * 2015-05-12 2021-12-30 Sangamo Therapeutics Inc Nuclease-mediated regulation of gene expression
HRP20240908T1 (hr) 2015-08-06 2024-10-25 The Curators Of The University Of Missouri Svinja i stanice otporne na virus reproduktivnog i respiratornog sindroma svinja (prrsv) koje imaju modificirane cd163 gene
AU2017212713B2 (en) 2016-01-27 2023-01-19 Oncorus, Inc. Oncolytic viral vectors and uses thereof
WO2017143071A1 (en) 2016-02-18 2017-08-24 The Regents Of The University Of California Methods and compositions for gene editing in stem cells
JP2019515654A (ja) 2016-03-16 2019-06-13 ザ ジェイ. デヴィッド グラッドストーン インスティテューツ 肥満及び/又は糖尿病を処置するための方法及び組成物、並びに候補処置薬剤を識別するための方法及び組成物
US11293033B2 (en) 2016-05-18 2022-04-05 Amyris, Inc. Compositions and methods for genomic integration of nucleic acids into exogenous landing pads
US11078481B1 (en) 2016-08-03 2021-08-03 KSQ Therapeutics, Inc. Methods for screening for cancer targets
IL264792B2 (en) * 2016-08-24 2023-10-01 Sangamo Therapeutics Inc Engineered target-specific zinc-finger nucleases
WO2018039440A1 (en) 2016-08-24 2018-03-01 Sangamo Therapeutics, Inc. Regulation of gene expression using engineered nucleases
US11078483B1 (en) 2016-09-02 2021-08-03 KSQ Therapeutics, Inc. Methods for measuring and improving CRISPR reagent function
RU2019121801A (ru) 2016-12-14 2021-01-15 Лигандал, Инк. Композиции и способы для доставки полезной нагрузки в виде нуклеиновой кислоты и/или белка
EP3579858A4 (en) 2017-02-07 2020-12-23 The Regents of The University of California GENE THERAPY AGAINST HAPLOINSUFFICIENCY
MY200418A (en) 2017-06-15 2023-12-25 Univ California Targeted non-viral dna insertions
MX2020000993A (es) 2017-07-26 2020-07-22 Oncorus Inc Vectores virales oncolíticos y usos de estos.
SG11202003798TA (en) 2017-10-27 2020-05-28 Univ California Targeted replacement of endogenous t cell receptors
WO2019125851A1 (en) 2017-12-18 2019-06-27 Syngenta Participations Ag Targeted insertion sites in the maize genome
IL326834A (en) 2018-10-02 2026-04-01 Sangamo Therapeutics Inc Artificial zinc finger protein transcription factors repressing mapt expression for the prevention and/or treatment of tauopathies
KR20210069692A (ko) * 2018-10-02 2021-06-11 상가모 테라퓨틱스, 인코포레이티드 조작된 유전자 조정제
US20200157531A1 (en) * 2018-11-16 2020-05-21 Wisconsin Alumni Research Foundation Method to enhance screening for homologous recombination in genome edited cells using recombination-activated fluorescent donor delivery vector
US12460230B2 (en) 2019-02-10 2025-11-04 The J. David Gladstone Institutes Modified mitochondrion and methods of use thereof
JP2022533713A (ja) 2019-05-21 2022-07-25 サンガモ セラピューティクス, インコーポレイテッド 調節性t細胞における制御された導入遺伝子発現
WO2021011936A2 (en) 2019-07-18 2021-01-21 University Of Rochester Cell-type selective immunoprotection of cells
WO2021028359A1 (en) 2019-08-09 2021-02-18 Sangamo Therapeutics France Controlled expression of chimeric antigen receptors in t cells
CN111462823B (zh) * 2020-04-08 2022-07-12 西安交通大学 一种基于dna测序数据的同源重组缺陷判定方法
US20240000051A1 (en) 2020-11-16 2024-01-04 Pig Improvement Company Uk Limited Influenza a-resistant animals having edited anp32 genes
TWI918825B (zh) 2021-02-10 2026-03-21 美商艾勒維生技公司 化合物、組合物及其使用方法
MX2024003887A (es) 2021-10-14 2024-07-09 Arsenal Biosciences Inc Células inmunitarias que tienen arnch coespresados y sistemas de compuerta lógica.
US20250043269A1 (en) 2021-11-03 2025-02-06 The J. David Gladstone Institutes, a testamentary trust established under the Will of J. David Glad Precise Genome Editing Using Retrons
US20250064032A1 (en) 2021-12-10 2025-02-27 Pig Improvement Company Uk Limited Editing tmprss2/4 for disease resistance in livestock
WO2023141602A2 (en) 2022-01-21 2023-07-27 Renagade Therapeutics Management Inc. Engineered retrons and methods of use
WO2024013514A2 (en) 2022-07-15 2024-01-18 Pig Improvement Company Uk Limited Gene edited livestock animals having coronavirus resistance
AU2023329392A1 (en) 2022-08-25 2025-02-27 Renagade Therapeutics Management Inc. Engineered retrons and methods of use
KR20250088783A (ko) 2022-11-04 2025-06-17 베링거 인겔하임 인터내셔날 게엠베하 전신 경화증을 치료하기 위한 가용성 구아닐레이트 사이클라제 활성제
EP4687956A1 (en) 2023-03-31 2026-02-11 Briacell Therapeutics Corp. Methods for enhancing the immunogenicity of cellular vaccines
EP4771154A2 (en) 2023-09-01 2026-07-08 Renagade Therapeutics Management Inc. Gene editing systems, compositions, and methods for treatment of vexas syndrome
WO2025174765A1 (en) 2024-02-12 2025-08-21 Renagade Therapeutics Management Inc. Lipid nanoparticles comprising coding rna molecules for use in gene editing and as vaccines and therapeutic agents

Family Cites Families (58)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0222493A1 (en) 1985-10-04 1987-05-20 Lubrizol Genetics Inc. TR-based sub-TI plasmids
US5422251A (en) 1986-11-26 1995-06-06 Princeton University Triple-stranded nucleic acids
ATE112314T1 (de) 1988-05-17 1994-10-15 Lubrizol Genetics Inc Pflanzliches ubiquitinpromotorsystem.
US5176996A (en) 1988-12-20 1993-01-05 Baylor College Of Medicine Method for making synthetic oligonucleotides which bind specifically to target sites on duplex DNA molecules, by forming a colinear triplex, the synthetic oligonucleotides and methods of use
FR2646438B1 (fr) 1989-03-20 2007-11-02 Pasteur Institut Procede de remplacement specifique d'une copie d'un gene present dans le genome receveur par l'integration d'un gene different de celui ou se fait l'integration
US5420032A (en) 1991-12-23 1995-05-30 Universitge Laval Homing endonuclease which originates from chlamydomonas eugametos and recognizes and cleaves a 15, 17 or 19 degenerate double stranded nucleotide sequence
US5356802A (en) 1992-04-03 1994-10-18 The Johns Hopkins University Functional domains in flavobacterium okeanokoites (FokI) restriction endonuclease
US5436150A (en) 1992-04-03 1995-07-25 The Johns Hopkins University Functional domains in flavobacterium okeanokoities (foki) restriction endonuclease
US5487994A (en) 1992-04-03 1996-01-30 The Johns Hopkins University Insertion and deletion mutants of FokI restriction endonuclease
US5792632A (en) 1992-05-05 1998-08-11 Institut Pasteur Nucleotide sequence encoding the enzyme I-SceI and the uses thereof
DK0604662T3 (da) 1992-07-07 2008-10-20 Japan Tobacco Inc Fremgangsmåde til transformering af monocotyledon
JP4012243B2 (ja) 1994-01-18 2007-11-21 ザ スクリップス リサーチ インスティチュート 亜鉛フィンガータンパク質誘導体およびそのための方法
US6140466A (en) 1994-01-18 2000-10-31 The Scripps Research Institute Zinc finger protein derivatives and methods therefor
US6242568B1 (en) 1994-01-18 2001-06-05 The Scripps Research Institute Zinc finger protein derivatives and methods therefor
US5585245A (en) 1994-04-22 1996-12-17 California Institute Of Technology Ubiquitin-based split protein sensor
GB9824544D0 (en) 1998-11-09 1999-01-06 Medical Res Council Screening system
CA2196419C (en) 1994-08-20 2007-08-21 Yen Choo Improvements in or relating to binding proteins for recognition of dna
US5789538A (en) * 1995-02-03 1998-08-04 Massachusetts Institute Of Technology Zinc finger proteins with high affinity new DNA binding specificities
US5773695A (en) 1996-01-26 1998-06-30 North Carolina State University Plant nuclear scaffold attachment region and method for increasing gene expression in transgenic cells
US5925523A (en) 1996-08-23 1999-07-20 President & Fellows Of Harvard College Intraction trap assay, reagents and uses thereof
GB9703369D0 (en) 1997-02-18 1997-04-09 Lindqvist Bjorn H Process
GB2338237B (en) 1997-02-18 2001-02-28 Actinova Ltd In vitro peptide or protein expression library
US6342345B1 (en) 1997-04-02 2002-01-29 The Board Of Trustees Of The Leland Stanford Junior University Detection of molecular interactions by reporter subunit complementation
GB9710807D0 (en) 1997-05-23 1997-07-23 Medical Res Council Nucleic acid binding proteins
GB9710809D0 (en) 1997-05-23 1997-07-23 Medical Res Council Nucleic acid binding proteins
EP0991764B1 (en) 1997-06-12 2006-07-12 Dow AgroSciences LLC Regulatory sequences for transgenic plants
PT1034262E (pt) * 1997-11-18 2005-10-31 Pioneer Hi Bred Int Composicoes e metodos para a modificacao genetica de plantas
US6506559B1 (en) 1997-12-23 2003-01-14 Carnegie Institute Of Washington Genetic inhibition by double-stranded RNA
AR014491A1 (es) 1998-01-29 2001-02-28 Dow Agrosciences Llc Metodo para obtener plantas transgenicas fertiles de gossypium hirsutum.
US6410248B1 (en) 1998-01-30 2002-06-25 Massachusetts Institute Of Technology General strategy for selecting high-affinity zinc finger proteins for diverse DNA target sites
DE69942334D1 (de) 1998-03-02 2010-06-17 Massachusetts Inst Technology Poly-zinkfinger-proteine mit verbesserten linkern
US6140081A (en) 1998-10-16 2000-10-31 The Scripps Research Institute Zinc finger binding domains for GNN
US6453242B1 (en) * 1999-01-12 2002-09-17 Sangamo Biosciences, Inc. Selection of sites for targeting by zinc finger proteins and methods of designing zinc finger proteins to bind to preselected sites
US6534261B1 (en) 1999-01-12 2003-03-18 Sangamo Biosciences, Inc. Regulation of endogenous gene expression in cells using zinc finger proteins
US6503717B2 (en) 1999-12-06 2003-01-07 Sangamo Biosciences, Inc. Methods of using randomized libraries of zinc finger proteins for the identification of gene function
US6794136B1 (en) 2000-11-20 2004-09-21 Sangamo Biosciences, Inc. Iterative optimization in the design of binding proteins
US7030215B2 (en) 1999-03-24 2006-04-18 Sangamo Biosciences, Inc. Position dependent recognition of GNN nucleotide triplets by zinc fingers
WO2001053480A1 (en) 2000-01-24 2001-07-26 Sangamo Biosciences, Inc. Nucleic acid binding polypeptides characterized by flexible linkers connected nucleic acid binding modules
US20020061512A1 (en) 2000-02-18 2002-05-23 Kim Jin-Soo Zinc finger domains and methods of identifying same
EP1276859B1 (en) 2000-04-28 2007-02-07 Sangamo Biosciences Inc. Targeted modification of chromatin structure
AU2001263155A1 (en) 2000-05-16 2001-11-26 Massachusetts Institute Of Technology Methods and compositions for interaction trap assays
JP2002060786A (ja) 2000-08-23 2002-02-26 Kao Corp 硬質表面用殺菌防汚剤
US7273923B2 (en) 2001-01-22 2007-09-25 Sangamo Biosciences, Inc. Zinc finger proteins for DNA binding and gene regulation in plants
GB0108491D0 (en) 2001-04-04 2001-05-23 Gendaq Ltd Engineering zinc fingers
AU2002250512A1 (en) 2001-04-05 2002-10-21 The Regents Of The University Of California Robotic device for locomotor training
US20040224385A1 (en) 2001-08-20 2004-11-11 Barbas Carlos F Zinc finger binding domains for cnn
WO2003054189A2 (de) 2001-12-20 2003-07-03 Sungene Gmbh & Co. Kgaa Verfahren zur transformation von pflanzlichen plastiden
US7262054B2 (en) 2002-01-22 2007-08-28 Sangamo Biosciences, Inc. Zinc finger proteins for DNA binding and gene regulation in plants
ATE347593T1 (de) * 2002-01-23 2006-12-15 Univ Utah Res Found Zielgerichtete chromosomale mutagenese mit zinkfingernukleasen
EP1504092B2 (en) 2002-03-21 2014-06-25 Sangamo BioSciences, Inc. Methods and compositions for using zinc finger endonucleases to enhance homologous recombination
US9447434B2 (en) 2002-09-05 2016-09-20 California Institute Of Technology Use of chimeric nucleases to stimulate gene targeting
US7888121B2 (en) 2003-08-08 2011-02-15 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
EP2927318B1 (en) 2003-08-08 2020-05-20 Sangamo Therapeutics, Inc. Methods and compositions for targeted cleavage and recombination
US20050102713A1 (en) 2003-11-07 2005-05-12 Merlo Donald J. Use of untranslated region of osmotin gene to enhance transgene expression in plants
EP1727906B1 (en) 2004-03-17 2012-02-22 BASF Plant Science GmbH Improved constructs for marker excision based on dual-function selection marker
US20080134351A1 (en) 2004-09-23 2008-06-05 Basf Plant Science Gmbh Recombination Cassettes and Methods For Sequence Excision in Plants
JP5639336B2 (ja) 2005-04-04 2014-12-10 バイエル・クロップサイエンス・エヌ・ヴェーBayer Cropscience N.V. 選ばれたdna配列を除去するための方法および手段
EP1913149A4 (en) 2005-07-26 2009-08-05 Sangamo Biosciences Inc TARGETED INTEGRATION AND EXPRESSION OF EXOGENOUS NUCLEIC ACID SEQUENCES

Also Published As

Publication number Publication date
PL2049663T3 (pl) 2015-08-31
US10669551B2 (en) 2020-06-02
US20160326537A1 (en) 2016-11-10
EP2049663B1 (en) 2015-02-25
US20100257638A1 (en) 2010-10-07
KR20090054986A (ko) 2009-06-01
WO2008021207A2 (en) 2008-02-21
JP2014193179A (ja) 2014-10-09
CN101528924B (zh) 2015-11-25
KR101439568B1 (ko) 2014-09-12
SI2049663T1 (sl) 2015-12-31
CA2660485C (en) 2016-04-12
CN101528924A (zh) 2009-09-09
AR062336A1 (es) 2008-10-29
EP2049663A1 (en) 2009-04-22
JP6442442B2 (ja) 2018-12-19
ZA200900787B (en) 2010-05-26
HK1134320A1 (zh) 2010-04-23
ES2532002T3 (es) 2015-03-23
CA2660485A1 (en) 2008-02-21
IL196942A (en) 2014-06-30
US9428756B2 (en) 2016-08-30
JP2016195606A (ja) 2016-11-24
IL196942A0 (en) 2011-08-01
JP2018029603A (ja) 2018-03-01
CN105296527A (zh) 2016-02-03
JP2010500029A (ja) 2010-01-07
CN105296527B (zh) 2020-11-27
AU2007284748B2 (en) 2013-05-16
JP6652952B2 (ja) 2020-02-26
TW200815593A (en) 2008-04-01
JP5964884B2 (ja) 2016-08-03
AR108684A2 (es) 2018-09-19
HK1221251A1 (zh) 2017-05-26
TWI424061B (zh) 2014-01-21
EP2395081A1 (en) 2011-12-14
AR105435A2 (es) 2017-10-04
BRPI0716427A2 (pt) 2014-03-11
DK2049663T3 (en) 2015-06-01
AU2007284748A1 (en) 2008-02-21

Similar Documents

Publication Publication Date Title
JP2010500029A5 (enExample)
Nazir et al. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated genome‐editing toolkit to enhance salt stress tolerance in rice and wheat
AU2018273986A1 (en) Bipartite base editor (BBE) architectures and type-II-C-Cas9 zinc finger editing
KR20190139318A (ko) 키메라 게놈 조작 분자 및 방법
DK1824970T3 (da) Thricoderma reesei-glycoamylase og homologer heraf
NZ602351A (en) Axmi-115, axmi-113, axmi-005, axmi-163 and axmi-184 : vip3a insecticidal proteins from bacillus thuringiensis and methods for their use
WO2012167192A3 (en) Methods and products for producing engineered mammalian cell lines with amplified transgenes
NO20091371L (no) Prosess for kromosomintegrasjon og DNA-sekvensutskiftning i Clostridia
CA3218040A1 (en) Orthogonal cas9 proteins for rna-guided gene regulation and editing
MX367170B (es) Enriquecimiento de la clasificacion de las celulas activadas por fluorescencia (facs) para generar plantas.
NO20013371L (no) Ekspresjon og eksport av anti-fedmeproteiner som Fc- fusjonsproteiner
WO2005099770A3 (en) Pancreatic islet microrna and methods for inhibiting same
NO20025402L (no) Modifiserte ES-celler og ES-cellespesifikke gener
ATE533838T1 (de) Verfahren zur verbesserten einführung von dna in bakterienzellen
WO2009118524A3 (en) Efficient insertion of dna into embryonic stem cells
DE602005021379D1 (de) System und verfahren zur produktion von antikörpern in der pflanzenzellkultur
MXPA06000092A (es) Transformacion de cloroplasto de la lenteja de agua.
JP2014523748A5 (enExample)
ATE555196T1 (de) Wärmebeständige duplexspezifische nuklease
WO2020006131A3 (en) Nucleases for genome editing
ATE546464T1 (de) Verfahren zur produktion von rekombinantem protein in cho-zellen
ATE452187T1 (de) Methode zur isolation von nukleinsäuren
WO2025096916A1 (en) Multi-site editing in living cells
JP7416745B2 (ja) 改変細胞、調製方法、及び構築物
CN112430586A (zh) 一种VI-B型CRISPR/Cas13基因编辑系统及其应用