ATE555196T1 - Wärmebeständige duplexspezifische nuklease - Google Patents
Wärmebeständige duplexspezifische nukleaseInfo
- Publication number
- ATE555196T1 ATE555196T1 AT09169259T AT09169259T ATE555196T1 AT E555196 T1 ATE555196 T1 AT E555196T1 AT 09169259 T AT09169259 T AT 09169259T AT 09169259 T AT09169259 T AT 09169259T AT E555196 T1 ATE555196 T1 AT E555196T1
- Authority
- AT
- Austria
- Prior art keywords
- specific nuclease
- protein
- duplex
- nuclease activity
- dsn
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2008226389 | 2008-09-03 | ||
JP2009131942A JP5517284B2 (ja) | 2008-09-03 | 2009-06-01 | 耐熱性2本鎖特異的核酸分解酵素 |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE555196T1 true ATE555196T1 (de) | 2012-05-15 |
Family
ID=41259451
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT09169259T ATE555196T1 (de) | 2008-09-03 | 2009-09-02 | Wärmebeständige duplexspezifische nuklease |
Country Status (4)
Country | Link |
---|---|
US (1) | US7951569B2 (de) |
EP (1) | EP2161331B1 (de) |
JP (1) | JP5517284B2 (de) |
AT (1) | ATE555196T1 (de) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2474225A (en) | 2009-07-21 | 2011-04-13 | Biotec Pharmacon Asa | DNase for decontamination of reverse transcription and amplification reactions |
WO2013003489A2 (en) * | 2011-06-27 | 2013-01-03 | University Of Florida Research Foundation, Inc | Method for genome complexity reduction and polymorphism detection |
WO2015049278A1 (en) * | 2013-10-01 | 2015-04-09 | Texcell | Detection of rare microbiological nucleic acids |
CN103882132A (zh) * | 2014-03-27 | 2014-06-25 | 中国人民解放军第三军医大学第一附属医院 | 痕量rna实时动态检测方法 |
JP2019154396A (ja) * | 2018-03-16 | 2019-09-19 | 株式会社リコー | 核酸検出方法、並びに、それに用いる標的核酸検出用プローブ、及び標的核酸検出用デバイス |
US11993792B2 (en) | 2021-05-27 | 2024-05-28 | New England Biolabs, Inc. | DNase I variants, compositions, methods, and kits |
CN114657202B (zh) * | 2022-03-30 | 2023-10-27 | 河南省儿童医院郑州儿童医院 | 一种耐热核酸降解酶表达载体、构建方法及其应用 |
CN116144630B (zh) * | 2022-10-31 | 2023-10-10 | 北京擎科生物科技股份有限公司 | 双链特异性核酸酶突变体及其应用 |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB9716664D0 (en) | 1997-08-06 | 1997-10-15 | Norwegian Inst Of Fisheries & | A method of removing nucleic acid contamination reactions |
AU2002346662A1 (en) | 2001-12-04 | 2003-06-17 | Euregen Llc | Methods and compositions for selectively cleaving dna containing duplex acids in a complex nucleic acid mixture |
JP2005218385A (ja) * | 2004-02-06 | 2005-08-18 | Dnaform:Kk | 1本鎖dnaを調製するための方法 |
JP2008226389A (ja) | 2007-03-15 | 2008-09-25 | Elpida Memory Inc | 半導体記憶装置 |
JP5134928B2 (ja) | 2007-11-30 | 2013-01-30 | 浜松ホトニクス株式会社 | 加工対象物研削方法 |
-
2009
- 2009-06-01 JP JP2009131942A patent/JP5517284B2/ja not_active Expired - Fee Related
- 2009-09-02 US US12/552,896 patent/US7951569B2/en not_active Expired - Fee Related
- 2009-09-02 AT AT09169259T patent/ATE555196T1/de active
- 2009-09-02 EP EP09169259A patent/EP2161331B1/de not_active Not-in-force
Also Published As
Publication number | Publication date |
---|---|
US7951569B2 (en) | 2011-05-31 |
US20100092976A1 (en) | 2010-04-15 |
JP2010081934A (ja) | 2010-04-15 |
JP5517284B2 (ja) | 2014-06-11 |
EP2161331B1 (de) | 2012-04-25 |
EP2161331A1 (de) | 2010-03-10 |
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