MX338911B - Identificacion mediada por trasposición de proteínas de unión o funcionales específicas. - Google Patents
Identificacion mediada por trasposición de proteínas de unión o funcionales específicas.Info
- Publication number
- MX338911B MX338911B MX2015000885A MX2015000885A MX338911B MX 338911 B MX338911 B MX 338911B MX 2015000885 A MX2015000885 A MX 2015000885A MX 2015000885 A MX2015000885 A MX 2015000885A MX 338911 B MX338911 B MX 338911B
- Authority
- MX
- Mexico
- Prior art keywords
- binding
- functional
- fragments
- host cells
- expressed proteins
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6854—Immunoglobulins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/55—Fab or Fab'
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
- C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
- C07K2317/622—Single chain antibody (scFv)
Abstract
La presente invención se refiere a un método de tecnología novedosa que ofrece eficiencia no paralela, flexibilidad, utilidad y velocidad para el descubrimiento y optimización de polipéptidos que tienen especificidad y/o funcionalidad de unión deseada, que incluye moléculas de unión al antígeno tales como anticuerpos y fragmentos de los mismos, para los fenotipos funcionales y/o de unión deseados. El método novedoso se base en constructos de transposición y diversas bibliotecas de ADN clonadas en vectores de transposición y su transfección en células huésped por expresión temporal concomitante de una enzima de transposasa funcional. Esto asegura una introducción eficiente y estable de los vectores de expresión basados en transposón en células huésped de vertebrado en una etapa, que pueden clasificarse entonces por un fenotipo funcional o de unión deseado de las proteínas expresadas, después de lo cual las secuencias de codificación relevantes para las proteínas expresadas, que incluyen anticuerpos y fragmentos de los mismos, pueden identificarse por técnicas de clonación y secuenciación de ADN estándar.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201261673144P | 2012-07-18 | 2012-07-18 | |
EP12178529.9A EP2692865B1 (en) | 2012-07-30 | 2012-07-30 | Transposition-mediated identification of specific binding or functional proteins |
PCT/EP2013/065214 WO2014013026A1 (en) | 2012-07-18 | 2013-07-18 | Transposition-mediated identification of specific binding or functional proteins |
Publications (2)
Publication Number | Publication Date |
---|---|
MX2015000885A MX2015000885A (es) | 2015-07-14 |
MX338911B true MX338911B (es) | 2016-05-05 |
Family
ID=49775745
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2015000885A MX338911B (es) | 2012-07-18 | 2013-07-18 | Identificacion mediada por trasposición de proteínas de unión o funcionales específicas. |
Country Status (19)
Country | Link |
---|---|
US (2) | US10174309B2 (es) |
EP (2) | EP2692865B1 (es) |
JP (1) | JP6043431B2 (es) |
CN (1) | CN104640985B (es) |
AU (1) | AU2013291971B2 (es) |
BR (1) | BR112015000836B1 (es) |
CA (1) | CA2878982C (es) |
DK (1) | DK2692865T3 (es) |
ES (1) | ES2528892T3 (es) |
HK (1) | HK1209779A1 (es) |
IL (1) | IL236771A (es) |
IN (1) | IN2015DN01301A (es) |
MX (1) | MX338911B (es) |
NZ (1) | NZ705074A (es) |
PT (1) | PT2692865E (es) |
SG (1) | SG11201500280XA (es) |
SI (1) | SI2692865T1 (es) |
WO (1) | WO2014013026A1 (es) |
ZA (1) | ZA201500203B (es) |
Families Citing this family (22)
Publication number | Priority date | Publication date | Assignee | Title |
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GB201407852D0 (en) | 2014-05-02 | 2014-06-18 | Iontas Ltd | Preparation of libraries od protein variants expressed in eukaryotic cells and use for selecting binding molecules |
EA201891066A1 (ru) | 2015-10-30 | 2018-10-31 | ЭнБиИ-ТЕРАПЬЮТИКС АГ | Антитела к ror1 |
WO2017127664A1 (en) | 2016-01-20 | 2017-07-27 | The Scripps Research Institute | Ror1 antibody compositions and related methods |
KR20180099887A (ko) | 2016-01-20 | 2018-09-05 | 더 스크립스 리서치 인스티튜트 | Ror2 항체 조성물 및 관련 방법 |
KR20180132705A (ko) * | 2016-04-04 | 2018-12-12 | 에테하 취리히 | 단백질 생산 및 라이브러리(Library) 생성을 위한 포유동물 세포주 |
WO2018107082A1 (en) | 2016-12-09 | 2018-06-14 | Gliknik Inc. | Methods of treating inflammatory disorders with multivalent fc compounds |
KR20190095929A (ko) | 2016-12-09 | 2019-08-16 | 글리크닉 인코포레이티드 | 다합체화 스트라도머인 gl-2045의 제조 최적화 |
US11278570B2 (en) | 2016-12-16 | 2022-03-22 | B-Mogen Biotechnologies, Inc. | Enhanced hAT family transposon-mediated gene transfer and associated compositions, systems, and methods |
KR102523318B1 (ko) | 2016-12-16 | 2023-04-18 | 비-모젠 바이오테크놀로지스, 인크. | 증진된 hAT 패밀리 트랜스포존 매개 유전자 전달 및 연관된 조성물, 시스템, 및 방법 |
WO2019016392A1 (en) | 2017-07-20 | 2019-01-24 | Nbe-Therapeutics Ag | HUMAN ANTIBODIES BINDING TO ROR2 |
SG11202000846WA (en) | 2017-08-07 | 2020-02-27 | Nbe Therapeutics Ag | Anthracycline-based antibody drug conjugates having high in vivo tolerability |
US20210214747A1 (en) * | 2018-05-18 | 2021-07-15 | Sorbonne Universite | Molecular tools and methods for transgene integration and their transposition dependent expression |
WO2019246486A2 (en) | 2018-06-21 | 2019-12-26 | B-Mogen Biotechnologies, Inc. | ENHANCED hAT FAMILY TRANSPOSON-MEDIATED GENE TRANSFER AND ASSOCIATED COMPOSITIONS, SYSTEMS, AND METHODS |
CN112912392A (zh) * | 2018-10-26 | 2021-06-04 | 豪夫迈·罗氏有限公司 | 利用重组酶介导的盒式交换的多特异性抗体筛选方法 |
JP2020146014A (ja) * | 2019-03-15 | 2020-09-17 | 株式会社東芝 | トランスポゼース活性測定用ベクター、トランスポゼース活性測定用キット、トランスポゼース活性測定方法及び細胞分離方法 |
GB2605276A (en) | 2019-09-03 | 2022-09-28 | Myeloid Therapeutics Inc | Methods and compositions for genomic integration |
CN111139264B (zh) * | 2020-01-20 | 2021-07-06 | 天津达济科技有限公司 | 一种基于线性双链dna分子在哺乳动物细胞系内构建单域抗体库的方法 |
CN111471714A (zh) * | 2020-05-07 | 2020-07-31 | 西南大学 | Minos转座子系统介导的真核生物转基因细胞系及构建方法 |
AU2021413365A1 (en) | 2020-12-30 | 2023-07-20 | Alaunos Therapeutics, Inc. | Recombinant vectors comprising polycistronic expression cassettes and methods of use thereof |
WO2022183167A1 (en) | 2021-02-25 | 2022-09-01 | Alaunos Therapeutics, Inc. | Recombinant vectors comprising polycistronic expression cassettes and methods of use thereof |
JP2024513871A (ja) * | 2021-03-31 | 2024-03-27 | ディーディーバイオ.カンパニー、リミテッド、(シャンハイ) | 機能性抗原結合タンパク質のスクリーニングに用いられるベクターおよび方法 |
WO2023150562A1 (en) | 2022-02-01 | 2023-08-10 | Alaunos Therapeutics, Inc. | Methods for activation and expansion of t cells |
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US5667988A (en) * | 1992-01-27 | 1997-09-16 | The Scripps Research Institute | Methods for producing antibody libraries using universal or randomized immunoglobulin light chains |
US6090382A (en) * | 1996-02-09 | 2000-07-18 | Basf Aktiengesellschaft | Human antibodies that bind human TNFα |
US6218185B1 (en) | 1996-04-19 | 2001-04-17 | The United States Of America As Represented By The Secretary Of Agriculture | Piggybac transposon-based genetic transformation system for insects |
EP0973928B1 (en) | 1997-03-11 | 2010-05-05 | Regents Of The University Of Minnesota | Dna-based transposon system for the introduction of nucleic acid into dna of a cell |
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US7105343B1 (en) | 2000-10-31 | 2006-09-12 | University Of Notre Dame Du Lac | Methods and compositions for transposition using minimal segments of the eukaryotic transformation vector Piggybac |
US6962810B2 (en) | 2000-10-31 | 2005-11-08 | University Of Notre Dame Du Lac | Methods and compositions for transposition using minimal segments of the eukaryotic transformation vector piggyBac |
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WO2003089618A2 (en) | 2002-04-22 | 2003-10-30 | Regents Of The University Of Minnesota | Transposon system and methods of use |
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WO2009039166A1 (en) | 2007-09-17 | 2009-03-26 | Affomix Corporation | INCREASING SPECIFICITY IN A scFV SCREEN USING DUAL BAIT REPORTERS |
US7863425B2 (en) * | 2007-09-26 | 2011-01-04 | Cornell University | Compositions and methods for inhibiting Yersinia pestis infection |
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WO2009087230A1 (en) | 2008-01-11 | 2009-07-16 | Morphosys Ag | Display vectors and methods and uses thereof |
EP2244563B1 (en) * | 2008-02-01 | 2019-08-28 | Bogin, Robert | Methods, compositions, and systems for production of recombinant spider silk polypeptides |
JP2009225781A (ja) * | 2008-02-29 | 2009-10-08 | Neosilk Co Ltd | カイコを用いた糖鎖構造に特徴を有する糖蛋白質の製造方法 |
EP2098536A1 (en) | 2008-03-05 | 2009-09-09 | 4-Antibody AG | Isolation and identification of antigen- or ligand-specific binding proteins |
WO2010069913A1 (en) | 2008-12-16 | 2010-06-24 | Novartis Ag | Yeast display systems |
US20100311116A1 (en) * | 2009-06-04 | 2010-12-09 | Excellgene Sa | Fast generation of high expression stable cell lines expressing recombinant proteins under minimal and short-term selective pressure |
CA2820151C (en) | 2010-12-15 | 2019-03-26 | Inter-University Research Institute Corporation Research Organization Of Information And Systems | Process for production of protein |
-
2012
- 2012-07-30 PT PT121785299T patent/PT2692865E/pt unknown
- 2012-07-30 DK DK12178529.9T patent/DK2692865T3/en active
- 2012-07-30 EP EP12178529.9A patent/EP2692865B1/en active Active
- 2012-07-30 ES ES12178529.9T patent/ES2528892T3/es active Active
- 2012-07-30 SI SI201230139T patent/SI2692865T1/sl unknown
-
2013
- 2013-07-18 EP EP13177083.6A patent/EP2687599A1/en not_active Withdrawn
- 2013-07-18 MX MX2015000885A patent/MX338911B/es active IP Right Grant
- 2013-07-18 US US14/414,981 patent/US10174309B2/en active Active
- 2013-07-18 CA CA2878982A patent/CA2878982C/en active Active
- 2013-07-18 WO PCT/EP2013/065214 patent/WO2014013026A1/en active Application Filing
- 2013-07-18 CN CN201380048308.7A patent/CN104640985B/zh active Active
- 2013-07-18 AU AU2013291971A patent/AU2013291971B2/en active Active
- 2013-07-18 BR BR112015000836-4A patent/BR112015000836B1/pt active IP Right Grant
- 2013-07-18 SG SG11201500280XA patent/SG11201500280XA/en unknown
- 2013-07-18 NZ NZ705074A patent/NZ705074A/en unknown
- 2013-07-18 JP JP2015522101A patent/JP6043431B2/ja active Active
-
2015
- 2015-01-13 ZA ZA2015/00203A patent/ZA201500203B/en unknown
- 2015-01-18 IL IL236771A patent/IL236771A/en active IP Right Grant
- 2015-02-17 IN IN1301DEN2015 patent/IN2015DN01301A/en unknown
- 2015-09-08 HK HK15108709.8A patent/HK1209779A1/xx unknown
-
2018
- 2018-10-30 US US16/175,073 patent/US20190153433A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
DK2692865T3 (en) | 2015-02-16 |
SI2692865T1 (sl) | 2015-03-31 |
SG11201500280XA (en) | 2015-02-27 |
BR112015000836B1 (pt) | 2021-12-21 |
CA2878982C (en) | 2017-01-03 |
US10174309B2 (en) | 2019-01-08 |
EP2692865A1 (en) | 2014-02-05 |
ZA201500203B (en) | 2015-12-23 |
WO2014013026A1 (en) | 2014-01-23 |
JP2015522286A (ja) | 2015-08-06 |
EP2692865B1 (en) | 2014-12-10 |
HK1209779A1 (en) | 2016-04-08 |
PT2692865E (pt) | 2015-02-06 |
IN2015DN01301A (es) | 2015-07-03 |
NZ705074A (en) | 2017-04-28 |
ES2528892T3 (es) | 2015-02-13 |
BR112015000836A2 (pt) | 2017-12-12 |
US20150152406A1 (en) | 2015-06-04 |
AU2013291971B2 (en) | 2016-02-04 |
JP6043431B2 (ja) | 2016-12-14 |
US20190153433A1 (en) | 2019-05-23 |
CA2878982A1 (en) | 2014-01-23 |
CN104640985A (zh) | 2015-05-20 |
EP2687599A1 (en) | 2014-01-22 |
AU2013291971A1 (en) | 2015-02-05 |
CN104640985B (zh) | 2021-05-11 |
IL236771A (en) | 2016-09-29 |
MX2015000885A (es) | 2015-07-14 |
WO2014013026A8 (en) | 2014-12-04 |
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