BR112015000836A2 - método para identificar um polipeptídeo tendo uma funcionalidade ou especificidade de ligação desejadas, biblioteca de moléculas de polinucleotídeo, método para gerar uma biblioteca de polinucleotídeos, vetor, célula hospedeira, anticorpo e método para gerar uma população de células hospedeiras - Google Patents

método para identificar um polipeptídeo tendo uma funcionalidade ou especificidade de ligação desejadas, biblioteca de moléculas de polinucleotídeo, método para gerar uma biblioteca de polinucleotídeos, vetor, célula hospedeira, anticorpo e método para gerar uma população de células hospedeiras

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BR112015000836A2
BR112015000836A2 BR112015000836A BR112015000836A BR112015000836A2 BR 112015000836 A2 BR112015000836 A2 BR 112015000836A2 BR 112015000836 A BR112015000836 A BR 112015000836A BR 112015000836 A BR112015000836 A BR 112015000836A BR 112015000836 A2 BR112015000836 A2 BR 112015000836A2
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generating
library
host cell
transposition
polynucleotide
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BR112015000836A
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BR112015000836B1 (pt
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Grawunder Ulf
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Nbe Therapeutics Llc
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • C—CHEMISTRY; METALLURGY
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    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)

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  • Health & Medical Sciences (AREA)
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  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
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  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

resumo “método para identificar um polipeptídeo tendo uma funcionalidade ou especificidade de ligação desejadas, biblioteca de moléculas de polinucleotídeo, método para gerar uma biblioteca de polinucleotídeos, vetor, célula hospedeira, anticorpo e método para gerar uma população de células hospedeiras” a presente invenção refere-se a um método que oferece eficiência, flexibilidade, utilidade e velocidade incomparáveis para a descoberta e otimização de polipeptídeos com funcionalidade e/ou especificidade de ligação desejadas, incluindo moléculas de ligação a antígeno, tais como anticorpos e fragmentos dos mesmos, para fenótipos funcionais e/ou de ligação desejados. o novo método baseia-se em construções de transposição e bibliotecas de adn diversificadas clonadas em vetores de transposição e em sua transfeção em células hospedeiras por expressão transitória concomitante de uma enzima transponase funcional. isto assegura uma introdução eficiente e estável de vetores de expressão baseados na transposição de células hospedeiras de vertebrados em uma etapa, que podem depois ser classificados em relação a um fenótipo funcional ou de ligação desejado das proteínas expressas, após o que as sequências codificantes relevantes para as proteínas expressas, incluindo anticorpos e fragmentos dos mesmos, podem ser identificadas por técnicas de clonagem e de sequenciação de adn convencionais. ?? ?? ?? ?? 1/1
BR112015000836-4A 2012-07-18 2013-07-18 Método para identificar um polipeptídeo tendo uma funcionalidade ou especificidade de ligação desejadas, biblioteca de moléculas de polinucleotídeo, método para gerar uma biblioteca de polinucleotídeos e método para gerar uma população de células hospedeiras BR112015000836B1 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261673144P 2012-07-18 2012-07-18
US61/673,144 2012-07-18
EP12178529.9A EP2692865B1 (en) 2012-07-30 2012-07-30 Transposition-mediated identification of specific binding or functional proteins
EP12178529-9 2012-07-30
PCT/EP2013/065214 WO2014013026A1 (en) 2012-07-18 2013-07-18 Transposition-mediated identification of specific binding or functional proteins

Publications (2)

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BR112015000836A2 true BR112015000836A2 (pt) 2017-12-12
BR112015000836B1 BR112015000836B1 (pt) 2021-12-21

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BR112015000836-4A BR112015000836B1 (pt) 2012-07-18 2013-07-18 Método para identificar um polipeptídeo tendo uma funcionalidade ou especificidade de ligação desejadas, biblioteca de moléculas de polinucleotídeo, método para gerar uma biblioteca de polinucleotídeos e método para gerar uma população de células hospedeiras

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US (2) US10174309B2 (pt)
EP (2) EP2692865B1 (pt)
JP (1) JP6043431B2 (pt)
CN (1) CN104640985B (pt)
AU (1) AU2013291971B2 (pt)
BR (1) BR112015000836B1 (pt)
CA (1) CA2878982C (pt)
DK (1) DK2692865T3 (pt)
ES (1) ES2528892T3 (pt)
HK (1) HK1209779A1 (pt)
IL (1) IL236771A (pt)
IN (1) IN2015DN01301A (pt)
MX (1) MX338911B (pt)
NZ (1) NZ705074A (pt)
PT (1) PT2692865E (pt)
SG (1) SG11201500280XA (pt)
SI (1) SI2692865T1 (pt)
WO (1) WO2014013026A1 (pt)
ZA (1) ZA201500203B (pt)

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DK2692865T3 (en) 2015-02-16
SI2692865T1 (sl) 2015-03-31
MX338911B (es) 2016-05-05
SG11201500280XA (en) 2015-02-27
BR112015000836B1 (pt) 2021-12-21
CA2878982C (en) 2017-01-03
US10174309B2 (en) 2019-01-08
EP2692865A1 (en) 2014-02-05
ZA201500203B (en) 2015-12-23
WO2014013026A1 (en) 2014-01-23
JP2015522286A (ja) 2015-08-06
EP2692865B1 (en) 2014-12-10
HK1209779A1 (en) 2016-04-08
PT2692865E (pt) 2015-02-06
IN2015DN01301A (pt) 2015-07-03
NZ705074A (en) 2017-04-28
ES2528892T3 (es) 2015-02-13
US20150152406A1 (en) 2015-06-04
AU2013291971B2 (en) 2016-02-04
JP6043431B2 (ja) 2016-12-14
US20190153433A1 (en) 2019-05-23
CA2878982A1 (en) 2014-01-23
CN104640985A (zh) 2015-05-20
EP2687599A1 (en) 2014-01-22
AU2013291971A1 (en) 2015-02-05
CN104640985B (zh) 2021-05-11
IL236771A (en) 2016-09-29
MX2015000885A (es) 2015-07-14
WO2014013026A8 (en) 2014-12-04

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