EA201200186A1 - Вектор, содержащий промотор маннозы, и промотор маннозы - Google Patents

Вектор, содержащий промотор маннозы, и промотор маннозы

Info

Publication number
EA201200186A1
EA201200186A1 EA201200186A EA201200186A EA201200186A1 EA 201200186 A1 EA201200186 A1 EA 201200186A1 EA 201200186 A EA201200186 A EA 201200186A EA 201200186 A EA201200186 A EA 201200186A EA 201200186 A1 EA201200186 A1 EA 201200186A1
Authority
EA
Eurasian Patent Office
Prior art keywords
vector
mannose
nucleic acid
acid sequence
mannose promoter
Prior art date
Application number
EA201200186A
Other languages
English (en)
Other versions
EA032045B1 (ru
Inventor
Тианги Сан
Йозеф Альтенбухнер
Кристоф Кициак
Original Assignee
Лонца Аг
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Лонца Аг filed Critical Лонца Аг
Publication of EA201200186A1 publication Critical patent/EA201200186A1/ru
Publication of EA032045B1 publication Critical patent/EA032045B1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Настоящее изобретение относится к вектору, способному экспрессироваться в прокариотном хозяине, и к последовательности нуклеиновых кислот, содержащим индуцируемый маннозой промотор оперона маннозы Bacillus subfiles, при этом указанные вектор и последовательность нуклеиновых кислот, соответственно, могут должным образом использоваться для трансформирования клетки-хозяина для экспрессии гетерологической последовательности нуклеиновых кислот, кодирующей полипептид в ходе, в частности, ферментации с высокой клеточной плотностью.
EA201200186A 2009-08-10 2010-08-02 ВЕКТОР, СОДЕРЖАЩИЙ ПРОМОТОР manP ИЛИ manR ОПЕРОНА МАННОЗЫ EA032045B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP09010283A EP2284273B1 (en) 2009-08-10 2009-08-10 Vector comprising mannose promoter and mannose promoter
PCT/EP2010/061193 WO2011018376A1 (en) 2009-08-10 2010-08-02 Vector comprising mannose promoter and mannose promoter

Publications (2)

Publication Number Publication Date
EA201200186A1 true EA201200186A1 (ru) 2012-06-29
EA032045B1 EA032045B1 (ru) 2019-03-29

Family

ID=41351667

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201200186A EA032045B1 (ru) 2009-08-10 2010-08-02 ВЕКТОР, СОДЕРЖАЩИЙ ПРОМОТОР manP ИЛИ manR ОПЕРОНА МАННОЗЫ

Country Status (21)

Country Link
US (1) US9163247B2 (ru)
EP (1) EP2284273B1 (ru)
JP (1) JP5794985B2 (ru)
KR (1) KR101877286B1 (ru)
CN (1) CN102471774B (ru)
AU (1) AU2010283807B2 (ru)
BR (1) BR112012002973B1 (ru)
CA (1) CA2770557C (ru)
CL (1) CL2012000363A1 (ru)
DK (1) DK2284273T3 (ru)
EA (1) EA032045B1 (ru)
ES (1) ES2394668T3 (ru)
HK (1) HK1167677A1 (ru)
IL (1) IL218025A0 (ru)
MX (1) MX317816B (ru)
PL (1) PL2284273T3 (ru)
PT (1) PT2284273E (ru)
SG (1) SG178292A1 (ru)
TW (1) TWI450965B (ru)
WO (1) WO2011018376A1 (ru)
ZA (1) ZA201200627B (ru)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2292769A1 (en) * 2009-08-10 2011-03-09 Lonza AG Fermentation process
EA024834B1 (ru) * 2010-07-29 2016-10-31 Лонца Аг Регуляция индуцируемых промоторов
US9970017B2 (en) * 2013-03-27 2018-05-15 Lonza Ag Regulation of inducible promoters
WO2017149065A1 (en) 2016-03-02 2017-09-08 Lonza Ltd Improved fermentation process
CN108277179B (zh) * 2018-03-07 2021-08-03 武汉生物样本库有限公司 一种枯草芽孢杆菌的高密度发酵及喷雾干燥方法
RU2719140C1 (ru) * 2019-07-26 2020-04-17 Общество с ограниченной ответственностью "ЦЕНТР ТРАНСФЕРА БИОТЕХНОЛОГИЙ ОКА-Биотех" Бактерия рода bacillus, продуцирующая гиалуроновую кислоту, и способ получения гиалуроновой кислоты с использованием указанной бактерии
RU2723722C1 (ru) * 2019-07-26 2020-06-17 Общество с ограниченной ответственностью "ЦЕНТР ТРАНСФЕРА БИОТЕХНОЛОГИЙ ОКА-Биотех" ТАНДЕМНЫЙ ПРОМОТОР, ФУНКЦИОНИРУЮЩИЙ В БАКТЕРИИ РОДА Bacillus, ТРАНСФОРМИРОВАННАЯ БАКТЕРИЯ РОДА Bacillus - ПРОДУЦЕНТ ЦЕЛЕВОГО ПРОДУКТА, СОДЕРЖАЩАЯ УКАЗАННЫЙ ПРОМОТОР, И СПОСОБ ПОЛУЧЕНИЯ ЦЕЛЕВОГО ПРОДУКТА С ИСПОЛЬЗОВАНИЕМ УКАЗАННОЙ БАКТЕРИИ

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10225380B4 (de) * 2002-06-07 2006-07-06 Technische Universität Braunschweig Produktion und Sekretion von Glucosyltransferasen
AU2003275416A1 (en) * 2002-10-04 2004-05-04 Embiosis Pharmaceuticals Compatible host/vector systems for expression of dna
KR101304921B1 (ko) * 2005-06-06 2013-09-13 피페넥스 인크. 세균 숙주 세포에서의 만니톨 유도 프로모터 시스템
US20070259004A1 (en) * 2005-12-08 2007-11-08 Universitaet Bayreuth Expression vector for Bacillus species
EP2292769A1 (en) * 2009-08-10 2011-03-09 Lonza AG Fermentation process

Also Published As

Publication number Publication date
ZA201200627B (en) 2012-09-26
PL2284273T3 (pl) 2013-03-29
US20120202246A1 (en) 2012-08-09
CN102471774B (zh) 2014-10-22
MX2012001672A (es) 2012-03-07
CA2770557C (en) 2019-01-22
EP2284273A1 (en) 2011-02-16
DK2284273T3 (da) 2013-01-21
HK1167677A1 (en) 2012-12-07
CN102471774A (zh) 2012-05-23
KR20120040740A (ko) 2012-04-27
TW201111504A (en) 2011-04-01
KR101877286B1 (ko) 2018-07-11
JP5794985B2 (ja) 2015-10-14
AU2010283807A1 (en) 2012-02-16
JP2013501510A (ja) 2013-01-17
WO2011018376A1 (en) 2011-02-17
TWI450965B (zh) 2014-09-01
BR112012002973A2 (pt) 2015-09-01
CL2012000363A1 (es) 2012-09-14
PT2284273E (pt) 2013-01-14
AU2010283807B2 (en) 2015-07-23
BR112012002973B1 (pt) 2021-02-09
EA032045B1 (ru) 2019-03-29
SG178292A1 (en) 2012-03-29
CA2770557A1 (en) 2011-02-17
EP2284273B1 (en) 2012-10-10
IL218025A0 (en) 2012-04-30
MX317816B (es) 2014-02-06
EP2284273A9 (en) 2011-10-26
US9163247B2 (en) 2015-10-20
ES2394668T3 (es) 2013-02-04

Similar Documents

Publication Publication Date Title
EA201200186A1 (ru) Вектор, содержащий промотор маннозы, и промотор маннозы
EA200901110A1 (ru) Рекомбинантная клетка-хозяин для получения соединения, представляющего интерес
MX337984B (es) Variantes de alfa amilasa y polinucleotidos que codifican la misma.
MY176332A (en) Multispecific antibody constructs
EP2536821A4 (en) IMPROVING THE ACTIVITY OF PROTEINS REQUIRING AGGREGATE FE-S
MX2014004598A (es) Variantes de alfa-amilasa y polinucleotidos que codifican las mismas.
EP4253534A3 (en) Alpha-amylase variants and polynucleotides encoding same
WO2013049121A3 (en) Recombinant phage and methods
MX2013003681A (es) Ácidos nucleicos manipulados y métodos de uso de los mismos.
MX353621B (es) Variantes de alfa-amilasa.
CL2010001425A1 (es) Proteina de union que comprende una cadena polipeptidica capaz de unir un par de antigenos; acido nucleico aislado que codifica dicha proteina de union; vector y celula huesped; metodo para producir proteina de union; composicion farmaceutica que comprende dicha proteina.
MX2013002709A (es) Moleculas de union a 4-1bb.
MX338911B (es) Identificacion mediada por trasposición de proteínas de unión o funcionales específicas.
MX353710B (es) Polipeptidos que tienen actividad de lisozima y polinucleotidos que los codifican.
MY186219A (en) Production of proteins in glutamine-free cell culture media
EA201100225A1 (ru) Новые регуляторные элементы
MX351565B (es) Colageno 7 y metodos relacionados.
IN2015DN02771A (ru)
MX2013003236A (es) Variantes de polipeptidos que tienen actividad potenciadora celulolitica y polinucleotidos que codifican a los mismos.
MX2013003237A (es) Variantes de polipeptidos que tienen actividad potenciadora celulolitica y polinucleotidos que codifican a los mismos.
WO2012102527A3 (ko) 조절자 Ｔ 세포에 특이적으로 존재하는 새로운 표면단백질 Ｌｒｉｇ-1의 용도
MX2014001594A (es) Polipeptidos que tienen actividad de celulasa y polinucleotidos que los codifican.
MX349517B (es) Polipeptidos que tienen actividad de proteasa y polinucleotidos que codifican los mismos.
EA201400192A1 (ru) Клетка, ферментирующая пентозы
IN2014DN10111A (ru)

Legal Events

Date Code Title Description
MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): AM AZ BY KZ KG MD TJ TM