ES2532002T3 - Recombinación homóloga mediada por nucleasas con dedos de zinc - Google Patents

Recombinación homóloga mediada por nucleasas con dedos de zinc Download PDF

Info

Publication number
ES2532002T3
ES2532002T3 ES07811226.5T ES07811226T ES2532002T3 ES 2532002 T3 ES2532002 T3 ES 2532002T3 ES 07811226 T ES07811226 T ES 07811226T ES 2532002 T3 ES2532002 T3 ES 2532002T3
Authority
ES
Spain
Prior art keywords
sequence
sequences
dna
cleavage
target
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES07811226.5T
Other languages
English (en)
Spanish (es)
Inventor
Qihua Cai
Jeffrey Miller
William Michael Ainley
Robbi Janette Garrison
Joseph F. Petolino
Beth Cori Rubin-Wilson
Lisa Lynn Schulenberg
Andrew Frederick Worden
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sangamo Therapeutics Inc
Corteva Agriscience LLC
Original Assignee
Dow AgroSciences LLC
Sangamo Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dow AgroSciences LLC, Sangamo Biosciences Inc filed Critical Dow AgroSciences LLC
Application granted granted Critical
Publication of ES2532002T3 publication Critical patent/ES2532002T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/14—Plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C07K2319/81—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor containing a Zn-finger domain for DNA binding

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
ES07811226.5T 2006-08-11 2007-08-09 Recombinación homóloga mediada por nucleasas con dedos de zinc Active ES2532002T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US83714706P 2006-08-11 2006-08-11
US837147P 2006-08-11
PCT/US2007/017748 WO2008021207A2 (en) 2006-08-11 2007-08-09 Zinc finger nuclease-mediated homologous recombination

Publications (1)

Publication Number Publication Date
ES2532002T3 true ES2532002T3 (es) 2015-03-23

Family

ID=38624400

Family Applications (1)

Application Number Title Priority Date Filing Date
ES07811226.5T Active ES2532002T3 (es) 2006-08-11 2007-08-09 Recombinación homóloga mediada por nucleasas con dedos de zinc

Country Status (19)

Country Link
US (2) US9428756B2 (enExample)
EP (2) EP2395081A1 (enExample)
JP (4) JP2010500029A (enExample)
KR (1) KR101439568B1 (enExample)
CN (2) CN105296527B (enExample)
AR (3) AR062336A1 (enExample)
AU (1) AU2007284748B2 (enExample)
BR (1) BRPI0716427A2 (enExample)
CA (1) CA2660485C (enExample)
DK (1) DK2049663T3 (enExample)
ES (1) ES2532002T3 (enExample)
HK (1) HK1221251A1 (enExample)
IL (1) IL196942A (enExample)
PL (1) PL2049663T3 (enExample)
SI (1) SI2049663T1 (enExample)
TW (1) TWI424061B (enExample)
UA (1) UA106193C2 (enExample)
WO (1) WO2008021207A2 (enExample)
ZA (1) ZA200900787B (enExample)

Families Citing this family (83)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100575485C (zh) 2002-01-23 2009-12-30 犹他大学研究基金会 使用锌指核酸酶的定向染色体诱变
US7528643B2 (en) * 2003-02-12 2009-05-05 Semiconductor Energy Laboratory Co., Ltd. Semiconductor device, electronic device having the same, and driving method of the same
EP2415872B1 (en) * 2006-12-14 2016-05-11 Dow AgroSciences LLC Optimized non-canonical zinc finger proteins
EP2167666A2 (en) 2007-06-29 2010-03-31 Pioneer Hi-Bred International Inc. Methods for altering the genome of a monocot plant cell
KR101657504B1 (ko) * 2007-09-27 2016-09-19 상가모 바이오사이언스 인코포레이티드 생물학적으로 활성인 뉴클레아제의 신속한 생체내 확인
CL2009000440A1 (es) 2008-02-29 2010-11-12 Monsanto Technology Llc Polinucleotidos que comprende un promotor de actina de arroz truncado ligado operativamente al gen cspb, cromosoma de maiz que comprende dicho policleotido, procedimiemto para la generacion de especies vegetales resisentes a la sequia, y alimento basado en semillas de dichas especies vegetales.
WO2009114321A2 (en) 2008-03-11 2009-09-17 Precision Biosciencs, Inc. Rationally-designed meganucleases for maize genome engineering
CA2720903C (en) 2008-04-14 2019-01-15 Sangamo Biosciences, Inc. Linear donor constructs for targeted integration
CA2734235C (en) * 2008-08-22 2019-03-26 Sangamo Biosciences, Inc. Methods and compositions for targeted single-stranded cleavage and targeted integration
AU2010234539B2 (en) * 2009-04-07 2015-04-09 Corteva Agriscience Llc Nanoparticle mediated delivery of sequence specific nucleases
US8598413B2 (en) 2009-08-19 2013-12-03 Dow AgroSciecnes, LLC. AAD-1 event DAS-40278-9, related transgenic corn lines, event-specific identification thereof, and methods of weed control involving AAD-1
UA109882C2 (uk) 2009-08-19 2015-10-26 Спосіб визначення зиготності рослини кукурудзи, що містить об'єкт das-40278-9 aad-1 кукурудзи
WO2011052539A1 (ja) * 2009-10-26 2011-05-05 独立行政法人農業生物資源研究所 遺伝的に改変された植物細胞の製造方法
EP2319872A1 (en) 2009-11-04 2011-05-11 BASF Plant Science GmbH Amylopectin type starch with enhanced retrogradation stability
KR101906605B1 (ko) 2009-11-24 2018-10-10 다우 아그로사이언시즈 엘엘씨 Ａａｄ-12 이벤트 416, 관련된 트랜스제닉 대두 계통, 및 이의 이벤트-특이적 확인
MX336846B (es) * 2010-01-22 2016-02-03 Sangamo Biosciences Inc Alteracion genomica dirigida.
CA2794037A1 (en) * 2010-03-22 2011-09-29 Philip Morris Products S.A. Modifying enzyme activity in plants
SG185367A1 (en) * 2010-04-26 2012-12-28 Sangamo Biosciences Inc Genome editing of a rosa locus using zinc-finger nucleases
US9175280B2 (en) 2010-10-12 2015-11-03 Sangamo Biosciences, Inc. Methods and compositions for treating hemophilia B
WO2012074868A2 (en) 2010-12-03 2012-06-07 Ms Technologies, Llc Optimized expression of glyphosate resistance encoding nucleic acid molecules in plant cells
CA2819684C (en) 2010-12-03 2024-05-07 Dow Agrosciences Llc Stacked herbicide tolerance event 8264.44.06.1, related transgenic soybean lines, and detection thereof
CN102174649B (zh) * 2011-01-18 2013-06-12 陕西师范大学 快速检测锌指核酸酶介导基因定点整合的方法
SG194089A1 (en) 2011-04-27 2013-11-29 Amyris Inc Methods for genomic modification
CN103796508B (zh) 2011-05-02 2017-05-03 内布拉斯加大学评议会 具有有用性状的植物和相关方法
US9187567B2 (en) 2011-05-18 2015-11-17 Parkinson's Institute Assay to determine LRRK2 activity in parkinson's disease
EP2535416A1 (en) 2011-05-24 2012-12-19 BASF Plant Science Company GmbH Development of phytophthora resistant potato with increased yield
BR102012019434B1 (pt) 2011-07-26 2021-11-09 Dow Agrosciences Llc Métodos de controle de pestes, de insetos, molécula e sequência de dna diagnóstica para o evento de soja 9582.814.19.1
CA3186126A1 (en) 2011-09-21 2013-03-28 Sangamo Biosciences, Inc. Methods and compositions for regulation of transgene expression
EP2612918A1 (en) 2012-01-06 2013-07-10 BASF Plant Science Company GmbH In planta recombination
WO2013112559A1 (en) 2012-01-23 2013-08-01 Dow Agrosciences Llc Cotton event pdab4468.19.10.3 detection method
BR102013002598B1 (pt) 2012-02-01 2022-05-31 Dow Agrosciences Llc Molécula de ácido nucleico, proteína quimérica, método para a produção de um material de planta transgênica e uso do mesmo
BR112014031891A2 (pt) 2012-06-19 2017-08-01 Univ Minnesota direcionamento genético nas plantas utilizando vírus de dna
UA119135C2 (uk) 2012-09-07 2019-05-10 ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі Спосіб отримання трансгенної рослини
UA118090C2 (uk) 2012-09-07 2018-11-26 ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі Спосіб інтегрування послідовності нуклеїнової кислоти, що представляє інтерес, у ген fad2 у клітині сої та специфічний для локусу fad2 білок, що зв'язується, здатний індукувати спрямований розрив
AR092482A1 (es) * 2012-09-07 2015-04-22 Dow Agrosciences Llc Enriquecimiento de la clasificacion de las celulas activadas por fluorescencia (facs) para generar plantas
HK1217732A1 (zh) 2012-09-07 2017-01-20 美国陶氏益农公司 Fad3性能基因座及相應的能夠誘導靶向斷裂的靶位點特異性結合蛋白
WO2014165612A2 (en) 2013-04-05 2014-10-09 Dow Agrosciences Llc Methods and compositions for integration of an exogenous sequence within the genome of plants
EP3008186B1 (en) 2013-06-14 2018-11-28 Cellectis Methods for non-transgenic genome editing in plants
US10767188B2 (en) 2013-09-25 2020-09-08 Nutech Ventures Methods and compositions for obtaining useful plant traits
UY35812A (es) 2013-11-04 2015-05-29 Dow Agrosciences Llc ?loci de maíz óptimos?.
JP6560203B2 (ja) * 2013-11-04 2019-08-14 ダウ アグロサイエンシィズ エルエルシー 遺伝子ターゲッティングのためのユニバーサルドナー系
UY35816A (es) 2013-11-04 2015-05-29 Dow Agrosciences Llc ?locus óptimos de la soja?.
UA120503C2 (uk) 2013-11-04 2019-12-26 Дау Агросайєнсиз Елелсі Спосіб одержання трансгенної клітини рослини кукурудзи
US9476065B2 (en) 2013-12-19 2016-10-25 Amyris, Inc. Methods for genomic integration
US10683513B2 (en) 2013-12-31 2020-06-16 Dow Agrosciences Llc Tissue-specific expression and hybrid plant production
ES2965286T3 (es) * 2014-07-14 2024-04-12 Univ Washington State Silenciamiento de NANOS que abate las células germinales
WO2016098078A2 (en) 2014-12-19 2016-06-23 Novartis Ag Dimerization switches and uses thereof
US20160281055A1 (en) 2015-03-20 2016-09-29 Dow Agrosciences Llc Incorporation of phosphatidylcholine in a media composition
US10808020B2 (en) * 2015-05-12 2020-10-20 Sangamo Therapeutics, Inc. Nuclease-mediated regulation of gene expression
EP3331355B1 (en) 2015-08-06 2024-04-03 The Curators of the University of Missouri Porcine reproductive and respiratory syndrome virus (prrsv)-resistant porcine and cells having modified cd163 genes
JP2019508063A (ja) 2016-01-27 2019-03-28 オンコラス， インコーポレイテッド 腫瘍溶解性ウイルスベクター及びその使用
EP3417061B1 (en) 2016-02-18 2022-10-26 The Regents of the University of California Methods and compositions for gene editing in stem cells
JP2019515654A (ja) 2016-03-16 2019-06-13 ザ ジェイ． デヴィッド グラッドストーン インスティテューツ 肥満及び／又は糖尿病を処置するための方法及び組成物、並びに候補処置薬剤を識別するための方法及び組成物
EP3458595A2 (en) 2016-05-18 2019-03-27 Amyris, Inc. Compositions and methods for genomic integration of nucleic acids into exogenous landing pads
US11078481B1 (en) 2016-08-03 2021-08-03 KSQ Therapeutics, Inc. Methods for screening for cancer targets
ES2901989T3 (es) * 2016-08-24 2022-03-24 Sangamo Therapeutics Inc Nucleasas específicas de objetivo modificadas
MX2019002207A (es) 2016-08-24 2019-07-15 Sangamo Therapeutics Inc Regulacion de la expresion genica usando nucleasas modificadas.
US11078483B1 (en) 2016-09-02 2021-08-03 KSQ Therapeutics, Inc. Methods for measuring and improving CRISPR reagent function
RU2019121992A (ru) 2016-12-14 2021-01-15 Лигандал, Инк. Способы и композиции для доставки полезной нагрузки в виде нуклеиновых кислот и белков
US11730828B2 (en) 2017-02-07 2023-08-22 The Regents Of The University Of California Gene therapy for haploinsufficiency
CA3067382A1 (en) 2017-06-15 2018-12-20 The Regents Of The University Of California Targeted non-viral dna insertions
SG11202000691XA (en) 2017-07-26 2020-02-27 Oncorus Inc Oncolytic viral vectors and uses thereof
IL305485B2 (en) 2017-10-27 2025-10-01 Univ California Targeted replacement of endogenous T cell receptors
EP4534678A3 (en) 2017-12-18 2025-06-18 Syngenta Crop Protection AG Targeted insertion sites in the maize genome
CA3115158A1 (en) * 2018-10-02 2020-04-09 Sangamo Therapeutics, Inc. Engineered genetic modulators
US12318427B2 (en) 2018-10-02 2025-06-03 Sangamo Therapeutics, Inc. Methods and compositions for modulation of tau proteins
US20200157531A1 (en) * 2018-11-16 2020-05-21 Wisconsin Alumni Research Foundation Method to enhance screening for homologous recombination in genome edited cells using recombination-activated fluorescent donor delivery vector
WO2020163856A1 (en) 2019-02-10 2020-08-13 The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Gladstone Modified mitochondrion and methods of use thereof
WO2020237045A1 (en) 2019-05-21 2020-11-26 Sangamo Therapeutics, Inc. Controlled transgene expression in regulatory t cells
EP3999627A2 (en) 2019-07-18 2022-05-25 University of Rochester Cell-type selective immunoprotection of cells
WO2021028359A1 (en) 2019-08-09 2021-02-18 Sangamo Therapeutics France Controlled expression of chimeric antigen receptors in t cells
CN111462823B (zh) * 2020-04-08 2022-07-12 西安交通大学 一种基于dna测序数据的同源重组缺陷判定方法
WO2022101641A1 (en) 2020-11-16 2022-05-19 Pig Improvement Company Uk Limited Influenza a-resistant animals having edited anp32 genes
TW202246213A (zh) 2021-02-10 2022-12-01 美商昂克諾斯公司 化合物、組合物及其使用方法
AU2022366987A1 (en) 2021-10-14 2024-05-16 Arsenal Biosciences, Inc. Immune cells having co-expressed shrnas and logic gate systems
WO2023081756A1 (en) 2021-11-03 2023-05-11 The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Gladstone Precise genome editing using retrons
US20250064032A1 (en) 2021-12-10 2025-02-27 Pig Improvement Company Uk Limited Editing tmprss2/4 for disease resistance in livestock
WO2023141602A2 (en) 2022-01-21 2023-07-27 Renagade Therapeutics Management Inc. Engineered retrons and methods of use
WO2024013514A2 (en) 2022-07-15 2024-01-18 Pig Improvement Company Uk Limited Gene edited livestock animals having coronavirus resistance
WO2024044723A1 (en) 2022-08-25 2024-02-29 Renagade Therapeutics Management Inc. Engineered retrons and methods of use
WO2024206821A1 (en) 2023-03-31 2024-10-03 Briacell Therapeutics Corp. Methods for enhancing the immunogenicity of cellular vaccines
WO2025049959A2 (en) 2023-09-01 2025-03-06 Renagade Therapeutics Management Inc. Gene editing systems, compositions, and methods for treatment of vexas syndrome
WO2025174765A1 (en) 2024-02-12 2025-08-21 Renagade Therapeutics Management Inc. Lipid nanoparticles comprising coding rna molecules for use in gene editing and as vaccines and therapeutic agents

Family Cites Families (58)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0222493A1 (en) 1985-10-04 1987-05-20 Lubrizol Genetics Inc. TR-based sub-TI plasmids
US5422251A (en) 1986-11-26 1995-06-06 Princeton University Triple-stranded nucleic acids
CA1339684C (en) 1988-05-17 1998-02-24 Peter H. Quail Plant ubquitin promoter system
US5176996A (en) 1988-12-20 1993-01-05 Baylor College Of Medicine Method for making synthetic oligonucleotides which bind specifically to target sites on duplex DNA molecules, by forming a colinear triplex, the synthetic oligonucleotides and methods of use
FR2646438B1 (fr) 1989-03-20 2007-11-02 Pasteur Institut Procede de remplacement specifique d'une copie d'un gene present dans le genome receveur par l'integration d'un gene different de celui ou se fait l'integration
US5420032A (en) 1991-12-23 1995-05-30 Universitge Laval Homing endonuclease which originates from chlamydomonas eugametos and recognizes and cleaves a 15, 17 or 19 degenerate double stranded nucleotide sequence
US5436150A (en) 1992-04-03 1995-07-25 The Johns Hopkins University Functional domains in flavobacterium okeanokoities (foki) restriction endonuclease
US5487994A (en) 1992-04-03 1996-01-30 The Johns Hopkins University Insertion and deletion mutants of FokI restriction endonuclease
US5356802A (en) 1992-04-03 1994-10-18 The Johns Hopkins University Functional domains in flavobacterium okeanokoites (FokI) restriction endonuclease
US5792632A (en) 1992-05-05 1998-08-11 Institut Pasteur Nucleotide sequence encoding the enzyme I-SceI and the uses thereof
EP1983056A1 (en) 1992-07-07 2008-10-22 Japan Tobacco Inc. Method for transforming monocotyledons
US6242568B1 (en) 1994-01-18 2001-06-05 The Scripps Research Institute Zinc finger protein derivatives and methods therefor
US6140466A (en) 1994-01-18 2000-10-31 The Scripps Research Institute Zinc finger protein derivatives and methods therefor
CA2181548C (en) 1994-01-18 2009-11-03 Carlos F. Barbas, Iii Zinc finger protein derivatives and methods therefor
US5585245A (en) 1994-04-22 1996-12-17 California Institute Of Technology Ubiquitin-based split protein sensor
GB9824544D0 (en) 1998-11-09 1999-01-06 Medical Res Council Screening system
AU698152B2 (en) 1994-08-20 1998-10-22 Gendaq Limited Improvements in or relating to binding proteins for recognition of DNA
US5789538A (en) 1995-02-03 1998-08-04 Massachusetts Institute Of Technology Zinc finger proteins with high affinity new DNA binding specificities
US5773695A (en) 1996-01-26 1998-06-30 North Carolina State University Plant nuclear scaffold attachment region and method for increasing gene expression in transgenic cells
US5925523A (en) 1996-08-23 1999-07-20 President & Fellows Of Harvard College Intraction trap assay, reagents and uses thereof
GB9703369D0 (en) 1997-02-18 1997-04-09 Lindqvist Bjorn H Process
GB2338237B (en) 1997-02-18 2001-02-28 Actinova Ltd In vitro peptide or protein expression library
US6342345B1 (en) 1997-04-02 2002-01-29 The Board Of Trustees Of The Leland Stanford Junior University Detection of molecular interactions by reporter subunit complementation
GB9710809D0 (en) 1997-05-23 1997-07-23 Medical Res Council Nucleic acid binding proteins
GB9710807D0 (en) 1997-05-23 1997-07-23 Medical Res Council Nucleic acid binding proteins
DK0991764T3 (da) 1997-06-12 2006-11-13 Dow Agrosciences Llc Regulatoriske sekvenser for transgene planter
AU760113C (en) * 1997-11-18 2004-04-22 Pioneer Hi-Bred International, Inc. Compositions and methods for genetic modification of plants
US6506559B1 (en) 1997-12-23 2003-01-14 Carnegie Institute Of Washington Genetic inhibition by double-stranded RNA
AR014491A1 (es) 1998-01-29 2001-02-28 Dow Agrosciences Llc Metodo para obtener plantas transgenicas fertiles de gossypium hirsutum.
US6410248B1 (en) 1998-01-30 2002-06-25 Massachusetts Institute Of Technology General strategy for selecting high-affinity zinc finger proteins for diverse DNA target sites
DE69942334D1 (de) 1998-03-02 2010-06-17 Massachusetts Inst Technology Poly-zinkfinger-proteine mit verbesserten linkern
US6140081A (en) 1998-10-16 2000-10-31 The Scripps Research Institute Zinc finger binding domains for GNN
US6534261B1 (en) 1999-01-12 2003-03-18 Sangamo Biosciences, Inc. Regulation of endogenous gene expression in cells using zinc finger proteins
US6453242B1 (en) 1999-01-12 2002-09-17 Sangamo Biosciences, Inc. Selection of sites for targeting by zinc finger proteins and methods of designing zinc finger proteins to bind to preselected sites
US7030215B2 (en) 1999-03-24 2006-04-18 Sangamo Biosciences, Inc. Position dependent recognition of GNN nucleotide triplets by zinc fingers
US6794136B1 (en) 2000-11-20 2004-09-21 Sangamo Biosciences, Inc. Iterative optimization in the design of binding proteins
WO2001040798A2 (en) 1999-12-06 2001-06-07 Sangamo Biosciences, Inc. Methods of using randomized libraries of zinc finger proteins for the identification of gene function
AU2001226935B2 (en) 2000-01-24 2006-06-22 Gendaq Limited Nucleic acid binding polypeptides characterized by flexible linkers connected nucleic acid binding modules
US20020061512A1 (en) 2000-02-18 2002-05-23 Kim Jin-Soo Zinc finger domains and methods of identifying same
ATE353361T1 (de) 2000-04-28 2007-02-15 Sangamo Biosciences Inc Gezielten modifikation der chromatinstruktur
AU2001263155A1 (en) 2000-05-16 2001-11-26 Massachusetts Institute Of Technology Methods and compositions for interaction trap assays
JP2002060786A (ja) 2000-08-23 2002-02-26 Kao Corp 硬質表面用殺菌防汚剤
AU2002241946B2 (en) 2001-01-22 2007-04-26 Sangamo Therapeutics, Inc. Modified zinc finger binding proteins
GB0108491D0 (en) 2001-04-04 2001-05-23 Gendaq Ltd Engineering zinc fingers
WO2002080809A2 (en) 2001-04-05 2002-10-17 The Regents Of The University Of California Robotic device for locomotor training
US20040224385A1 (en) 2001-08-20 2004-11-11 Barbas Carlos F Zinc finger binding domains for cnn
CA2470329A1 (en) 2001-12-20 2003-07-03 Sungene Gmbh & Co. Kgaa Methods for the transformation of vegetal plastids
US7262054B2 (en) 2002-01-22 2007-08-28 Sangamo Biosciences, Inc. Zinc finger proteins for DNA binding and gene regulation in plants
CN100575485C (zh) 2002-01-23 2009-12-30 犹他大学研究基金会 使用锌指核酸酶的定向染色体诱变
WO2003080809A2 (en) 2002-03-21 2003-10-02 Sangamo Biosciences, Inc. Methods and compositions for using zinc finger endonucleases to enhance homologous recombination
CA2497913C (en) 2002-09-05 2014-06-03 California Institute Of Technology Use of chimeric nucleases to stimulate gene targeting
EP3222715A1 (en) 2003-08-08 2017-09-27 Sangamo BioSciences, Inc. Methods and compositions for targeted cleavage and recombination
US7888121B2 (en) 2003-08-08 2011-02-15 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
US20050102713A1 (en) 2003-11-07 2005-05-12 Merlo Donald J. Use of untranslated region of osmotin gene to enhance transgene expression in plants
EP1727906B1 (en) 2004-03-17 2012-02-22 BASF Plant Science GmbH Improved constructs for marker excision based on dual-function selection marker
WO2006032426A2 (en) 2004-09-23 2006-03-30 Basf Plant Science Gmbh Recombination cassettes and methods for sequence excision in plants
AU2006232828B2 (en) 2005-04-04 2011-01-27 Bayer Cropscience Nv. Methods and means for removal of a selected DNA sequence
CN101273141B (zh) 2005-07-26 2013-03-27 桑格摩生物科学股份有限公司 外源核酸序列的靶向整合和表达

Also Published As

Publication number Publication date
EP2049663B1 (en) 2015-02-25
CN101528924A (zh) 2009-09-09
JP2010500029A (ja) 2010-01-07
TWI424061B (zh) 2014-01-21
AU2007284748A1 (en) 2008-02-21
JP2014193179A (ja) 2014-10-09
AR062336A1 (es) 2008-10-29
JP2016195606A (ja) 2016-11-24
JP5964884B2 (ja) 2016-08-03
HK1221251A1 (zh) 2017-05-26
ZA200900787B (en) 2010-05-26
JP2018029603A (ja) 2018-03-01
AU2007284748B2 (en) 2013-05-16
CA2660485A1 (en) 2008-02-21
US20160326537A1 (en) 2016-11-10
SI2049663T1 (sl) 2015-12-31
BRPI0716427A2 (pt) 2014-03-11
CN105296527A (zh) 2016-02-03
TW200815593A (en) 2008-04-01
IL196942A (en) 2014-06-30
JP6652952B2 (ja) 2020-02-26
AR105435A2 (es) 2017-10-04
WO2008021207A2 (en) 2008-02-21
PL2049663T3 (pl) 2015-08-31
HK1134320A1 (zh) 2010-04-23
US10669551B2 (en) 2020-06-02
JP6442442B2 (ja) 2018-12-19
CN105296527B (zh) 2020-11-27
EP2049663A1 (en) 2009-04-22
IL196942A0 (en) 2011-08-01
DK2049663T3 (en) 2015-06-01
US9428756B2 (en) 2016-08-30
UA106193C2 (ru) 2014-08-11
US20100257638A1 (en) 2010-10-07
CA2660485C (en) 2016-04-12
KR20090054986A (ko) 2009-06-01
CN101528924B (zh) 2015-11-25
AR108684A2 (es) 2018-09-19
KR101439568B1 (ko) 2014-09-12
EP2395081A1 (en) 2011-12-14

Similar Documents

Publication Publication Date Title
ES2532002T3 (es) Recombinación homóloga mediada por nucleasas con dedos de zinc
ES2654923T3 (es) Integración dirigida en la localización Zp15
JP5902631B2 (ja) 標的化ゲノム変更
CN101878307B (zh) 以5‑烯醇式丙酮酰莽草酸‑3‑磷酸合酶基因为靶的改造锌指蛋白
AU2017216446B2 (en) Zinc finger nuclease-mediated homologous recombination
AU2013216688B2 (en) Zinc finger nuclease-mediated homologous recombination
HK1134320B (en) Zinc finger nuclease-mediated homologous recombination
BRPI0716427B1 (pt) Method for introducing an exogenous sequence in the genome of a plant cell