AP2004003122A0 - Cloning of cytochrome P450 genes from nicotiana - Google Patents

Cloning of cytochrome P450 genes from nicotiana

Info

Publication number
AP2004003122A0
AP2004003122A0 APAP/P/2004/003122A AP2004003122A AP2004003122A0 AP 2004003122 A0 AP2004003122 A0 AP 2004003122A0 AP 2004003122 A AP2004003122 A AP 2004003122A AP 2004003122 A0 AP2004003122 A0 AP 2004003122A0
Authority
AP
ARIPO
Prior art keywords
nicotiana
cytochrome
cloning
genes
enzymes
Prior art date
Application number
APAP/P/2004/003122A
Inventor
Dongmei Xu
Original Assignee
Us Smokeless Tobacco Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Us Smokeless Tobacco Co filed Critical Us Smokeless Tobacco Co
Publication of AP2004003122A0 publication Critical patent/AP2004003122A0/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)

Abstract

The present invention relates to P450 enzymes and nucleic acid sequences encoding P450 enzymes in nicotiana, and methods of using those enzymes and nucleic acid sequences to alter plant phenotypes.
APAP/P/2004/003122A 2002-03-12 2003-03-12 Cloning of cytochrome P450 genes from nicotiana AP2004003122A0 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US36368402P 2002-03-12 2002-03-12
PCT/US2003/007430 WO2003078577A2 (en) 2002-03-12 2003-03-12 Cloning of cytochrome p450 genes from nicotiana

Publications (1)

Publication Number Publication Date
AP2004003122A0 true AP2004003122A0 (en) 2004-09-30

Family

ID=28041798

Family Applications (1)

Application Number Title Priority Date Filing Date
APAP/P/2004/003122A AP2004003122A0 (en) 2002-03-12 2003-03-12 Cloning of cytochrome P450 genes from nicotiana

Country Status (10)

Country Link
EP (1) EP1572911A4 (en)
JP (2) JP2005531297A (en)
KR (1) KR20040091124A (en)
CN (1) CN101166824A (en)
AP (1) AP2004003122A0 (en)
AU (1) AU2003220165A1 (en)
BR (1) BR0308351A (en)
CA (1) CA2477452A1 (en)
MX (1) MXPA04008785A (en)
WO (1) WO2003078577A2 (en)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7855318B2 (en) 2001-11-13 2010-12-21 U.S. Smokeless Tobacco Company Cloning of cytochrome P450 genes from Nicotiana
US7700834B2 (en) 2001-11-13 2010-04-20 U.S. Smokless Tobacco Company Nicotiana nucleic acid molecules and uses thereof
US10266836B2 (en) 2001-11-13 2019-04-23 U.S. Smokeless Tobacco Company Llc Tobacco nicotine demethylase genomic clone and uses thereof
US7700851B2 (en) * 2001-11-13 2010-04-20 U.S. Smokeless Tobacco Company Tobacco nicotine demethylase genomic clone and uses thereof
US7812227B2 (en) 2001-11-13 2010-10-12 U.S. Smokeless Tobacco Company Cloning of cytochrome p450 genes from nicotiana
US8592663B2 (en) 2001-11-13 2013-11-26 U.S. Smokeless Tobacco Company Llc Tobacco nicotine demethylase genomic clone and uses thereof
CN1867674B (en) 2003-10-16 2011-11-09 美国无烟烟草有限责任公司 Cloning of cytochrome P450 genes from nicotiana
ES2377868T3 (en) * 2003-10-16 2012-04-02 U.S. Smokeless Tobacco Company Llc Cloning of genes encoding cytochrome p450 from Nicotiana
BRPI0415362A (en) * 2003-10-16 2006-12-12 Us Smokeless Tobacco Co nicotine-isolated nucleic acid molecule, nicotine-isolated protein, transgenic plant, methods of producing a transgenic plant, selecting a plant containing a nucleic acid molecule, and increasing or decreasing nornicotine levels in a plant , tobacco product and leaf having reduced amounts of nornicotin levels, and method of isolating a gene from a plant
US8586837B2 (en) 2004-04-29 2013-11-19 U.S. Smokeless Tobacco Company Llc Nicotiana nucleic acid molecules and uses thereof
AU2011232795B2 (en) * 2004-04-29 2015-05-28 U.S. Smokeless Tobacco Company Llc Nicotiana nucleic acid molecules and uses thereof
CN103348912B (en) * 2004-04-29 2016-07-13 美国无烟烟草有限责任公司 Nicotiana nucleic acid molecule and application thereof
CN101384710B (en) * 2004-04-29 2012-05-02 美国无烟烟草有限责任公司 Nicotine demethylase genenome clone and uses thereof
JP4753937B2 (en) * 2004-04-29 2011-08-24 ユーエス スモークレス タバコ カンパニー リミテッド ライアビリティ カンパニー Nicotiana nucleic acid molecule and use thereof
JP5106103B2 (en) * 2004-04-29 2012-12-26 ユーエス スモークレス タバコ カンパニー リミテッド ライアビリティ カンパニー Tobacco nicotine demethylase genomic clone and its use
PL2295549T3 (en) * 2004-04-29 2015-10-30 U S Smokeless Tobacco Company Llc Nicotiana nucleic acid molecules and uses thereof
CN101979583B (en) * 2004-04-29 2016-08-10 美国无烟烟草有限责任公司 Nicotiana nucleic acid molecules and application thereof
ATE530656T1 (en) 2005-02-23 2011-11-15 Univ North Carolina State MODIFICATION OF ALKALOID CONTENT IN TOBACCO THROUGH MODIFICATION OF SPECIFIC CYTOCHROME P450 GENES.
JP2010512733A (en) * 2006-12-13 2010-04-30 ディーエスエム アイピー アセッツ ビー．ブイ． Method for preparing pravastatin
US8319011B2 (en) 2006-12-15 2012-11-27 U.S. Smokeless Tobacco Company Llc Tobacco plants having reduced nicotine demethylase activity
US11332753B2 (en) 2006-12-15 2022-05-17 U.S. Smokeless Tobacco Company Llc Tobacco plants having reduced nicotine demethylase activity
CN101939431B (en) 2007-11-12 2015-01-28 北卡罗来纳州立大学 Alteration of tobacco alkaloid content through modification of specific cytochrome P450 genes
EA033565B1 (en) 2010-01-15 2019-11-05 Univ North Carolina State Compositions and methods for minimizing nornicotine synthesis in tobacco plants
KR20140004760A (en) * 2011-02-28 2014-01-13 노쓰 캐롤라이나 스테이트 유니버시티 Tobacco inbred plants ncbex1f, ncbex1ms, and nc ex90
KR20140034160A (en) 2011-02-28 2014-03-19 알트리아 클라이언트 서비시스 인코포레이티드 Tobacco inbred plants albex1f and albex1ms
CN105517430A (en) 2013-01-11 2016-04-20 北卡罗莱纳州立大学 Tobacco inbred plants K326 SRC, CMS K326 SRC, K346 SRC, CMS K346 SRC, NC1562-1 SRC, NCTG-61 SRC, CMS NCTG-61 SRC AND HYBRID NC196 SRC
WO2014137802A1 (en) 2013-03-05 2014-09-12 North Carolina State University Tobacco inbred and hybrid plants and uses thereof
US9596822B2 (en) 2014-03-03 2017-03-21 North Carolina State University Tobacco inbred and hybrid plants and tobacco products made thereof
US9596823B2 (en) 2014-03-03 2017-03-21 North Carolina State University Tobacco inbred and hybrid plants and tobacco products made thereof
CN106659232A (en) 2014-03-03 2017-05-10 北卡罗莱纳州立大学 Tobacco inbred and hybrid plants and tobacco products made thereof
KR102201082B1 (en) 2018-10-30 2021-01-08 인하대학교 산학협력단 The method of generating oxygen vacancies in nickel-cobalt oxide for oxygen reduction reaction and nickel-cobalt oxide thereby

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1373476B1 (en) * 2001-03-09 2011-06-15 University Of Kentucky Research Foundation Cytochrome p450s and uses thereof
AU2003301431A1 (en) * 2002-10-16 2004-05-04 U.S. Smokeless Tobacco Company Cloning of cytochrome p450 genes from nicotiana

Also Published As

Publication number Publication date
EP1572911A2 (en) 2005-09-14
KR20040091124A (en) 2004-10-27
BR0308351A (en) 2007-04-17
JP2005531297A (en) 2005-10-20
JP2010131027A (en) 2010-06-17
AU2003220165A1 (en) 2003-09-29
CN101166824A (en) 2008-04-23
CA2477452A1 (en) 2003-09-25
WO2003078577A2 (en) 2003-09-25
EP1572911A4 (en) 2008-06-25
AU2003220165A8 (en) 2008-03-06
WO2003078577A8 (en) 2008-01-10
MXPA04008785A (en) 2005-09-30
WO2003078577A9 (en) 2004-01-22

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