MX2021002265A - Composiciones y métodos para aumentar la edición genética basada en oligonucleótidos de donador. - Google Patents

Composiciones y métodos para aumentar la edición genética basada en oligonucleótidos de donador.

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Publication number
MX2021002265A
MX2021002265A MX2021002265A MX2021002265A MX2021002265A MX 2021002265 A MX2021002265 A MX 2021002265A MX 2021002265 A MX2021002265 A MX 2021002265A MX 2021002265 A MX2021002265 A MX 2021002265A MX 2021002265 A MX2021002265 A MX 2021002265A
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Mexico
Prior art keywords
cell
compositions
methods
donor oligonucleotide
gene editing
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Application number
MX2021002265A
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English (en)
Inventor
Elias Quijano
Audrey Turchick
Peter Glazer
Original Assignee
Univ Yale
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Publication date
Application filed by Univ Yale filed Critical Univ Yale
Publication of MX2021002265A publication Critical patent/MX2021002265A/es

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    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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Abstract

Se describen composiciones para la edición de genes mejorada y métodos de uso de las mismas. La composición contiene un anticuerpo que penetra en las células y un oligonucleótido donante que contiene una secuencia que puede corregir una mutación en el genoma de una célula. Preferiblemente, la composición no contiene nucleasa, PNA o nanopartícula. Las composiciones se usan para modificar el genoma de una célula al poner en contacto la célula con una cantidad eficaz de la composición. La modificación genómica se produce con mayor frecuencia tanto ex vivo como in vivo, cuando las células se ponen en contacto con el anticuerpo que penetra a las células y el oligonucleótido del donante en comparación con la ausencia del anticuerpo que penetra en las células.
MX2021002265A 2018-08-31 2019-08-30 Composiciones y métodos para aumentar la edición genética basada en oligonucleótidos de donador. MX2021002265A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862725920P 2018-08-31 2018-08-31
PCT/US2019/048953 WO2020047344A1 (en) 2018-08-31 2019-08-30 Compositions and methods for enhancing donor oligonucleotide-based gene editing

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MX2021002265A true MX2021002265A (es) 2021-05-27

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US (2) US20210340280A1 (es)
EP (1) EP3844287B1 (es)
JP (1) JP2022500009A (es)
KR (1) KR20210053930A (es)
CN (1) CN112930399A (es)
AU (1) AU2019331009A1 (es)
BR (1) BR112021003818A2 (es)
CA (1) CA3111518A1 (es)
IL (1) IL281108A (es)
MX (1) MX2021002265A (es)
SG (1) SG11202101975RA (es)
WO (1) WO2020047344A1 (es)

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