WO2012144859A3 - 세포 환경 내에서의 단일 분자 수준의 단백질-단백질 상호작용 분석 방법 및 장치 - Google Patents

세포 환경 내에서의 단일 분자 수준의 단백질-단백질 상호작용 분석 방법 및 장치 Download PDF

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WO2012144859A3
WO2012144859A3 PCT/KR2012/003077 KR2012003077W WO2012144859A3 WO 2012144859 A3 WO2012144859 A3 WO 2012144859A3 KR 2012003077 W KR2012003077 W KR 2012003077W WO 2012144859 A3 WO2012144859 A3 WO 2012144859A3
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protein
molecule level
analyzing
interaction
marker
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PCT/KR2012/003077
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French (fr)
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WO2012144859A2 (ko
WO2012144859A9 (ko
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윤태영
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한국과학기술원
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Priority to JP2014506337A priority Critical patent/JP5903486B2/ja
Priority to CN201280026166.XA priority patent/CN103608677B/zh
Priority to EP12774021.5A priority patent/EP2700947B1/en
Publication of WO2012144859A2 publication Critical patent/WO2012144859A2/ko
Publication of WO2012144859A9 publication Critical patent/WO2012144859A9/ko
Publication of WO2012144859A3 publication Critical patent/WO2012144859A3/ko
Priority to US14/059,349 priority patent/US9423400B2/en
Priority to US15/211,096 priority patent/US9733255B2/en
Priority to US15/642,427 priority patent/US9964544B2/en
Priority to US15/945,890 priority patent/US10401367B2/en

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    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/648—Specially adapted constructive features of fluorimeters using evanescent coupling or surface plasmon coupling for the excitation of fluorescence

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Abstract

단일 분자 수준까지의 단백질-단백질 상호작용 분석 방법 및 분석 장치가 개시된다. 본 발명은, 제1 단백질에 구비된 제1 표지자와 결합되는 생체 분자체가 부착된 기판에 제1 단백질을 공급하여 제1 표지자와 생체 분자체의 결합을 유도하고, 제1 표지자와 생체 분자체가 결합된 경우에, 제2 표지자가 구비된 제2 단백질이 포함된 세포의 세포 용액을 기판에 공급하며, 세포 용액이 기판에 공급된 상태에서, 제1 단백질과 제2 단백질의 상호작용을 분석하는 과정을 통해 구현된다. 본 발명에 따르면, 실제 세포 환경 내에서의 단백질-단백질 상호작용을 단일 분자 수준까지 분석할 수 있게 된다.
PCT/KR2012/003077 2011-04-20 2012-04-20 세포 환경 내에서의 단일 분자 수준의 단백질-단백질 상호작용 분석 방법 및 장치 WO2012144859A2 (ko)

Priority Applications (7)

Application Number Priority Date Filing Date Title
JP2014506337A JP5903486B2 (ja) 2011-04-20 2012-04-20 細胞環境内でのタンパク質−タンパク質相互作用分析方法及び装置
CN201280026166.XA CN103608677B (zh) 2011-04-20 2012-04-20 在单分子水平上在细胞环境中分析蛋白-蛋白相互作用的方法和装置
EP12774021.5A EP2700947B1 (en) 2011-04-20 2012-04-20 Method for analyzing protein-protein interaction on single-molecule level within the cellular environment
US14/059,349 US9423400B2 (en) 2011-04-20 2013-10-21 Method and apparatus for analyzing protein-protein interaction on single-molecule level within the cellular environment
US15/211,096 US9733255B2 (en) 2011-04-20 2016-07-15 Method and apparatus for analyzing protein-protein interaction on single-molecule level within the cellular environment
US15/642,427 US9964544B2 (en) 2011-04-20 2017-07-06 Method and apparatus for analyzing protein-protein interaction on single-molecule level within the cellular environment
US15/945,890 US10401367B2 (en) 2011-04-20 2018-04-05 Method and apparatus for analyzing protein-protein interaction on single molecule level within the cellular environment

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
KR10-2011-0036942 2011-04-20
KR20110036942 2011-04-20
KR1020110088084A KR101167649B1 (ko) 2011-04-20 2011-08-31 세포 환경 내에서의 단일 분자 수준의 단백질-단백질 상호작용 분석 장치
KR1020110088062A KR101158362B1 (ko) 2011-04-20 2011-08-31 세포 환경 내에서의 단일 분자 수준의 단백질-단백질 상호작용 분석 방법
KR10-2011-0088062 2011-08-31
KR10-2011-0088084 2011-08-31

Related Child Applications (1)

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US14/059,349 Continuation US9423400B2 (en) 2011-04-20 2013-10-21 Method and apparatus for analyzing protein-protein interaction on single-molecule level within the cellular environment

Publications (3)

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WO2012144859A2 WO2012144859A2 (ko) 2012-10-26
WO2012144859A9 WO2012144859A9 (ko) 2013-01-31
WO2012144859A3 true WO2012144859A3 (ko) 2013-03-21

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PCT/KR2012/003087 WO2012144861A2 (ko) 2011-04-20 2012-04-20 세포 환경 내에서의 단일 분자 수준의 단백질-단백질 상호작용 분석 방법 및 세포 용액에서의 활성화된 단백질 농도 측정 방법
PCT/KR2012/003077 WO2012144859A2 (ko) 2011-04-20 2012-04-20 세포 환경 내에서의 단일 분자 수준의 단백질-단백질 상호작용 분석 방법 및 장치

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PCT/KR2012/003087 WO2012144861A2 (ko) 2011-04-20 2012-04-20 세포 환경 내에서의 단일 분자 수준의 단백질-단백질 상호작용 분석 방법 및 세포 용액에서의 활성화된 단백질 농도 측정 방법

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US (6) US9423400B2 (ko)
EP (2) EP2700950B1 (ko)
JP (4) JP5903486B2 (ko)
KR (5) KR101158362B1 (ko)
CN (3) CN103582817B (ko)
WO (2) WO2012144861A2 (ko)

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