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Definitions

• Novel substituted imidazo-pyridinones and imidazo-pyridazinones their preparation and their use as pharmaceuticals
• the present invention relates to novel substituted imidazopyridinones and imidazo-pyridazinones of the general formula
• DPP-IV dipeptidyl peptidase-IV
• the Manufacture their use for the prevention or treatment of diseases or conditions associated with increased DPP-IV activity or which can be prevented or alleviated by reduction of DPP-IV activity, in particular diabetes mellitus type I or type II , the medicament containing a compound of the general formula (I) or a physiologically acceptable salt thereof and processes for their preparation.
• the present invention thus relates to the above compounds of general formula I, which have valuable pharmacological properties, their tautomers, their enantiomers, their diastereomers, their mixtures and their salts, the medicaments containing the pharmacologically active compounds, their use and processes for their preparation ,
• R 5 represents a hydrogen atom or a methyl group
• R 1 is a 5- to 7-membered cycloalkyleneimino group which is substituted in the carbon skeleton by an amino group and may be substituted by a C 1-3 -alkyl group,
• R 2 is a benzyl group in which the phenyl radical may be substituted by one or two fluorine, chlorine or bromine atoms or by a cyano group,
• R 3 is a linear or branched C ⁇ - 6 alkyl group, an optionally substituted in the aryl moiety by a halogen atom, a cyano, a C1.3-AI- kyl or a methoxy phenyl-C ⁇ . 3- alkyl or naphthyl-C 1-3 -alkyl group,
• phenyl group by a hydroxy, C ⁇ - 3 alkyloxy, aminocarbonyl C ⁇ . 3 -alkoxy-, (C ⁇ - 3 -alkylamino) -carbonyl-C ⁇ - 3 -alkoxy, [di- (C ⁇ - 3 -alkyl) - amino] -carbonyl-C ⁇ - 3 -alkoxy-, amino, -C- 3- alkyl-carbonylamino, C 3 - 6 -cycloalkyl-carbonylamino, -C. 3- alkoxy-carbonylamino, C 1-3 -alkylsulfonylamino or aminocarbonyl group may be substituted,
• heteroaryl group is to be understood as meaning a monocyclic 5- or 6-membered heteroaryl group which is optionally substituted by one or two C 1-3 -alkyl groups or by a morpholin-4-yl, pyridyl or phenyl group,
• the 6-membered heteroaryl group contains one, two or three nitrogen atoms and
• the 5-membered heteroaryl group is an imino group optionally substituted by a C 1-3 -alkyl or phenyl-C 1-4 -alkyl group, an oxygen or
• a phenyl ring which may optionally be substituted by a halogen atom, by one or two C ⁇ alkyl groups or by a trifluoromethyl or methoxy group, may be fused
• the bond can be via one atom of the heterocyclic part or the fused phenyl ring,
• R is in each case a hydrogen atom or a methyl group
• R 4 is a hydrogen atom or a C 1-3 -alkyl group
• alkyl and alkoxy groups which have more than two carbon atoms and are not mentioned otherwise in the definitions can be straight-chain or branched,
• X is a nitrogen atom or a methine group
• R 1 is a piperazin-1-yl, 3-amino-piperidin-1-yl, 3-amino-3-methyl-piperidin-1-yl, 3-amino-pyrrolidin-1-yl, 1, 4 Diazepan-1-yl, (2-amino-cyclohexyl) -amino or piperidin-3-yl-amino group
• R 2 is a benzyl group in which the phenyl radical may be substituted by one or two fluorine atoms, by a chlorine or bromine atom or by a cyano group,
• R 3 is a linear or branched C ⁇ - 6 alkyl group
• a phenyl optionally substituted in the aryl part by a fluorine, chlorine or bromine atom or by a cyano, C ⁇ -Alky! - or methoxy group.
• phenyl group by a hydroxy, C ⁇ - 3 alkyloxy, Aminocarbono- nyl -C ⁇ . 3 -alkoxy-, (C ⁇ - 3 -alkylamino) carbonyl -C ⁇ . 3 alkoxy, [di- (3 C ⁇ - alkyl) - aminoj-carbonyl-C ⁇ s-alkoxy, amino, C ⁇ -3-alkyl-carbonylamino, C 3 - 6 -cycloalkyl-carbonylamino , C 1 -C 3 -alkoxycarbonylamino, C 1-3 -alkylsulfonylamino or aminocarbonyl group may be substituted,
• heteroaryl group is a pyridinyl, pyrimidinyl, pyridazinyl, pyrazinyl, thiazolyl, oxazolyl, isothiazolyl, isoxazolyl, pyrazolyl, imidazolyl optionally substituted by one or two methyl groups or by a pyridyl or phenyl group , Oxadiazolyl, thiadiazolyl or thienyl group,
• a phenyl ring which may optionally be substituted by a chlorine atom, by one or two methyl groups or by a trifluoromethyl or methoxy group, may be condensed
• the bond can be via one atom of the heterocyclic part or the fused phenyl ring,
• R 6 is in each case a hydrogen atom or a methyl group
• R 4 represents a hydrogen atom or a methyl group
• alkyl and alkoxy groups which have more than two carbon atoms and are not mentioned otherwise in the definitions can be straight-chain or branched,
• R 1 represents a 3-amino-piperidin-1-yl group, their tautomers, their enantiomers, their diastereomers, their mixtures and their salts.
• a second subgroup of preferred compounds relates to those compounds of general formula I in which
• R 1 , R 3 and R 4 are defined as mentioned above and
• R 2 represents a 3-methylallyl, a 3,3-dimethylallyl or a but-2-yn-4-yl group
• the compounds of general formula I are obtained by processes known per se, for example by the following processes:
• Z 1 is a nucleofuge leaving group such as a chlorine or bromine atom or a -C. 3- alkylsulfanyl, C ⁇ - 3 -alkylsulfinyl- or C ⁇ - 3 -alkylsulfonyl group, with an amine of the general formula HR 1 (III),
• the reaction is conveniently carried out in a solvent such as isopropanol, butanol, tetrahydrofuran, dioxane, dimethylformamide, dimethyl sulfoxide, ethylene glycol monomethyl ether, ethylene glycol diethyl ether or sulfolane optionally in the presence of an inorganic or tertiary organic base, eg sodium carbonate, potassium carbonate or potassium hydroxide, a tertiary organic base
• an inorganic or tertiary organic base eg sodium carbonate, potassium carbonate or potassium hydroxide, a tertiary organic base
• triethylamine or in the presence of N-ethyl-diisopropylamine (Hünig's base), which organic bases can also serve as a solvent, and optionally in the presence of a reaction accelerator such as an alkali halide or a palladium-based catalyst at temperatures between -20 and 180 ° C, but preferably at temperatures between
• R 2 , R 3 and R 4 are defined as mentioned above and R is one of the groups mentioned in the introduction for R 1 , in which the imino, amino or alkylamino group is substituted by a protective group.
• R is one of the groups mentioned in the introduction for R 1 , in which the imino, amino or alkylamino group is substituted by a protective group.
• protective groups are a variety of groups in question. For a review of protecting group chemistry, see Theodora W. Greene and Peter GM Wuts, Protective Groups in Organic Synthesis, Second Edition, 1991, John Wiley and Sons, and Philip J. Kocienski, Protecting Groups, Georg Thieme Verlag, 1994 ,
• protective groups include:
• the tert-butyloxycarbonyl group which is obtained by treatment with an acid such as trifluoroacetic acid or hydrochloric acid or by treatment with bromotrimethylsilane or iodotrimethylsilane optionally with a solvent such as methylene chloride, ethyl acetate, dioxane, methanol, isopropanol or diethyl ether at temperatures between 0 and 80 ° C split off,
• the carbobenzyloxycarbonyl group which can be removed, for example, by hydrogenolysis in the presence of a noble metal catalyst such as palladium-carbon and a solvent such as alcohols, ethyl acetate, dioxane, tetrahydrofuran or mixtures of these solvents at temperatures between 0 ° C and the boiling point of the solvent, by treatment with Boron tribromide in methylene chloride at temperatures between -20 ° C and room temperature, or by treatment with aluminum chloride / anisole at temperatures between 0 ° C and room temperature.
• a noble metal catalyst such as palladium-carbon
• a solvent such as alcohols, ethyl acetate, dioxane, tetrahydrofuran or mixtures of these solvents at temperatures between 0 ° C and the boiling point of the solvent
• Boron tribromide in methylene chloride at temperatures between -20 ° C and room temperature
• aluminum chloride / anisole at temperatures between 0 °
• a protective moiety used during the reactions to protect reactive groups is subsequently split off and / or a compound of the general formula I thus obtained is separated into its stereoisomers and / or
• optionally present reactive groups such as hydroxyl, carboxy, phosphono, O-alkyl phosphono, amino, alkylamino or imino groups can be protected during the reaction by conventional protecting groups, which after the reaction again be split off.
• the protective radical for a hydroxy group is the trimethylsilyl, acetyl, benzoyl, methyl, ethyl, tert-butyl, trityl, benzyl or tetrahydropyranyl group,
• protecting groups for a phosphono group an alkyl group such as the methyl, ethyl, isopropyl or n-butyl group, the phenyl or benzyl group and
• protecting groups for an amino, alkylamino or imino group the formyl, acetyl, trifluoroacetyl, ethoxycarbonyl, tert-butoxycarbonyl, benzyloxycarbonyl, benzyl, methoxybenzyl or 2,4-dimethoxybenzyl group and for the amino group additionally the phthalyl group into consideration.
• the optional subsequent cleavage of a protective moiety used is carried out, for example hydrolytically in an aqueous solvent, for example in water, isopropanol / water, acetic acid / water, tetrahydrofuran / water or dioxane / water, in the presence of an acid such as trifluoroacetic acid, hydrochloric acid or Sulfuric acid or in the presence of an alkali metal base such as sodium hydroxide or potassium hydroxide or aprotic, for example in the presence of iodotrimethylsilane, at temperatures between 0 and 120 ° C, preferably at temperatures between 10 and 100 ° C.
• an aqueous solvent for example in water, isopropanol / water, acetic acid / water, tetrahydrofuran / water or dioxane / water, in the presence of an acid such as trifluoroacetic acid, hydrochloric acid or Sulfuric acid or in the presence of
• cleavage of a benzyl, methoxybenzyl or benzyloxycarbonyl radical is for example effected by hydrogenolysis, e.g. with hydrogen in the presence of a catalyst such as palladium / carbon in a suitable solvent such as methanol, ethanol, ethyl acetate or glacial acetic acid optionally with addition of an acid such as hydrochloric acid at temperatures between 0 and 100 ° C, but preferably at room temperatures between 20 and 60 ° C. , and at a hydrogen pressure of 1 to 7 bar, but preferably from 3 to 5 bar.
• the cleavage of a 2,4-dimethoxybenzyl radical is preferably carried out in trifluoroacetic acid in the presence of anisole.
• tert-butyl or tert-Butyloxycarbonylrestes is preferably carried out by treatment with an acid such as trifluoroacetic acid or hydrochloric acid or by treatment with iodotrimethylsilane optionally with the use of a solvent such as methylene chloride, dioxane, methanol or diethyl ether.
• an acid such as trifluoroacetic acid or hydrochloric acid
• iodotrimethylsilane optionally with the use of a solvent such as methylene chloride, dioxane, methanol or diethyl ether.
• Trifluoracetylrestes preferably takes place by treatment with an acid such as hydrochloric acid optionally in the presence of a solvent such as acetic acid at temperatures between 50 and 120 ° C or by treatment with sodium hydroxide, optionally in the presence of a solvent such as tetrahydrofuran at temperatures between 0 and 50 ° C. ,
• the cleavage of a Phthalylrestes preferably takes place in the presence of hydrazine or a primary amine such as methylamine, ethylamine or n-butylamine in a solvent such as methanol, ethanol, isopropanol, toluene / water or dioxane at temperatures between 20 and 50 ° C.
• a primary amine such as methylamine, ethylamine or n-butylamine
• a solvent such as methanol, ethanol, isopropanol, toluene / water or dioxane at temperatures between 20 and 50 ° C.
• the cleavage of only one alkyl radical from an O.O'-dialkylphosphono group takes place, for example, with sodium iodide in a solvent such as acetone, ethyl methyl ketone, acetonitrile or dimethylformamide at temperatures between 40 and 150 ° C, but preferably at temperatures between 60 and 100 ° C.
• a solvent such as acetone, ethyl methyl ketone, acetonitrile or dimethylformamide
• the compounds of general formula I obtained can be separated into their enantiomers and / or diastereomers.
• cis / trans mixtures can be separated into their cis and trans isomers, and compounds having at least one optically active carbon atom can be resolved into their enantiomers.
• the cis- / trans mixtures obtained can be purified by chromatography into their cis and trans isomers, the compounds of general formula I which are obtained in racemates, by methods known per se (see Allinger NL and Eliel EL in " Topics in Stereochemistry ", Vol. 6, Wiley Interscience, 1971) in their optical antipodes and compounds of general formula I having at least 2 asymmetric carbon atoms due to their physicochemical differences according to known methods, eg by chromatography and / or fractional crystallization, into their diastereomers, which, if they are obtained in racemic form, can then be separated into the enantiomers as mentioned above.
• the enantiomer separation is preferably carried out by column separation on chiral phases or by recrystallization from an optically active solvent or by reacting with a, with the racemic compound salts or derivatives such as esters or amides forming optically active substance, in particular acids and their activated derivatives or alcohols, and separating the diastereomeric salt mixture or derivative thus obtained, for example due to different solubilities, wherein from the pure diastereomeric salts or derivatives, the free antipodes can be released by the action of suitable agents.
• optically active acids are, for example, the D and L forms of tartaric acid or dibenzoyltartaric acid, di-Op-toluoyl-tartaric acid, malic acid, mandelic acid, camphorsulfonic acid, glutamic acid, aspartic acid or quinic acid.
• optically active alcohols are (+) - or (-) - menthol and, for example, (+) - or (-) - menthyloxycarbonyl as the optically active acyl radical in amides.
• the compounds of the formula I obtained can be converted into their salts, in particular for pharmaceutical use, into their physiologically tolerated salts with inorganic or organic acids.
• suitable acids are hydrochloric acid, hydrobromic acid, sulfuric acid, methanesulfonic acid, phosphoric acid, fumaric acid, succinic acid, lactic acid, citric acid, tartaric acid or maleic acid.
• novel compounds of the formula I thus obtained can, if desired, subsequently be converted into their salts with inorganic or organic bases, in particular for the pharmaceutical application, into their physiologically tolerated salts.
• Suitable bases are, for example, sodium hydroxide, potassium hydroxide, arginine, cyclohexylamine, ethanolamine, diethanolamine and triethanolamine.
• the compounds of the general formula I according to the invention and their physiologically tolerated salts have valuable pharmacological properties, in particular an inhibiting action on the enzyme DPP-IV.
• the ability of the substances and their corresponding salts to inhibit DPP-IV activity can be demonstrated in an experimental set-up using an extract of the human colon carcinoma cell line Caco-2 as a DPP IV source.
• Differentiation of cells to induce DPP-IV expression was performed as described by Chandlerr et al. in an article entitled "Increased expression of intestinal cell line Caco-2", published in Proc. Natl. Acad. Be. Vol. 90, pages 5757-5761 (1993).
• the cell extract was purified from in a buffer (10 mM Tris HCl, 0.15 M NaCl, 0.04 t.i.u. aprotinin, 0.5%.
• Nonidet-P40, pH 8.0 solubilized cells by centrifugation at 35,000 g for 30 minutes at 4 ° C (to remove cell debris).
• the DPP-IV assay was performed as follows:
• 50 ⁇ l substrate solution (AFC, amido-4-trifluoromethylcoumarin AFC), final concentration 100 ⁇ M
• 20 ⁇ l assay buffer (final concentrations 50 mM Tris HCl pH 7.8, 50 mM NaCl, 1% DMSO) was pipetted.
• the reaction was started by adding 30 ⁇ l solubilized Caco-2 protein (final concentration 0.14 ⁇ g protein per well).
• the test substances to be tested were typically added prediluted in 20 ⁇ l, with the assay buffer volume correspondingly reduced. The reaction was carried out at room temperature, the incubation time was 60 minutes.
• the compounds prepared according to the invention are well tolerated, since, for example, no changes in the behavior of the animals could be observed after oral administration of 10 mg / kg of the compound of Example 2 to rats.
• the compounds of general formula I according to the invention and their corresponding pharmaceutically acceptable salts are capable of affecting all those conditions or diseases which may be affected by inhibition of DPP-IV activity . It is therefore to be expected that the compounds according to the invention can be used for the prevention or treatment of diseases or conditions such as diabetes mellitus type I and type II, diabetic complications, metabolic acidosis or ketosis, insulin resistance, dyslipidaemias of various genesis, arthritis, atherosclerosis and related diseases, obesity, Allograft transplantation and by caicitonin-induced osteoporosis are suitable. In addition, these substances are suitable for preventing B cell degeneration such as apoptosis or necrosis of pancreatic B cells.
• diseases or conditions such as diabetes mellitus type I and type II, diabetic complications, metabolic acidosis or ketosis, insulin resistance, dyslipidaemias of various genesis, arthritis, atherosclerosis and related diseases, obesity, Allograft transplantation and by caicitonin-induced osteoporosis are
• the substances are still suitable net to improve or restore the functionality of pancreatic cells, in addition to increase the number and size of pancreatic B cells.
• the compounds of this invention will be useful to provide, inter alia, a sedative or anxiolytic effect
• catabolic states after surgery or hormonal stress responses can be favorably influenced or the mortality and morbidity after myocardial infarction can be reduced.
• they are suitable for treating all conditions related to the above-mentioned effects mediated by GLP-1 or GLP-2.
• the compounds according to the invention are likewise usable as diuretics or antihypertensives and are suitable for the prevention and treatment of acute renal failure. Likewise, they are suitable for the prevention and treatment of chronic inflammatory bowel disease. Moreover, it is expected that DPP-IV inhibitors, and thus also the compounds of the present invention, can be used to treat infertility or improve human or mammalian fertility, particularly if infertility is associated with insulin resistance or polycystic ovarian syndrome , Furthermore, the substances are capable of affecting growth hormone deficiency states associated with shortage growth.
• Suitable therapeutics for such a combination include, for example, antidiabetics, such as metformin, sulfonylureas (eg glibenclamide, tolbutamide, glimepiride), nateglinides, repaglinide, thiazolidinediones (eg rosiglitazone, pioglitazone), PPAR-gamma agonists (eg Gl 262570) , alpha-glucosidase inhibitors (eg acarbose, voglibose), alpha2-antagonists, insulin and insulin analogs, GLP-1 and GLP-1 analogues (eg exendin-4) or amylin.
• antidiabetics such as metformin, sulfonylureas (eg glibenclamide, tolbutamide, glimepiride), nateglinides, repaglinide, thiazolidinediones (e
• inhibitors of protein tyrosine phosphatase 1 substances that affect a deregulated glucose production in the liver, such as inhibitors of glucose-6-phosphatase, or the fructose-1, 6-bisphosphatase, glycogen phosphorylase, glucagon receptor antagonists and inhibitors of Phosphoenolpyruvate carboxykinase, glycogen synthase kinase or pyruvate dehydrokinase, lipid lowering agents such as HMG-CoA reductase inhibitors (eg simvastatin, atorvastatin), fibrates (eg bezafibrate, fenofibrate), nicotinic acid and its derivatives, cholesterol resorption inhibitors such as ezetimibe, bile acid binding substances such as co-styramine, HDL-increasing compounds such as inhibitors of CETP or regulators of ABC1 or drugs for the treatment of obesity, such as sibutramine or te
• the dosage required to achieve a corresponding effect is expediently when intravenously administered 1 to 100 mg, preferably 1 to 30 mg, and when administered orally 1 to 1000 mg, preferably 1 to 100 mg, in each case 1 to 4 times daily.
• the compounds of formula I according to the invention optionally in combination with other active substances, together with one or more inert conventional carriers and / or diluents, e.g.
• lactose cane sugar, microcrystalline cellulose, magnesium stearate, polyvinylpyrrolidone, citric acid, tartaric acid, water, water / ethanol, water / glycerol, water / sorbitol, water / polyethylene glycol, propylene glycol, cetylstearyl alcohol, carboxymethylcellulose or fatty substances such as hard fat or their suitable mixtures, in common pharmaceutical preparations such as tablets, dragees, capsules, powders, suspensions or suppositories incorporate.
• Washed 1 N sodium hydroxide solution then dried over sodium sulfate and evaporated.
• Example 139 2 ((R) -3-Amino-piperidin-1-vn-3- (but-2-ynyl) -5- (benzodiisoxazol-3-ylmethyl) -3,5-dihydro-imidazof4.5-d1pyridazine-4 -one
• Example 196 2 ((?) - 3-Amino-piperidin-1-yl) -3- (but-2-ynyl) -5- (5-chlorobenzoxazol-2-ylmethyl) -3,5-dihydro-imidazof4, 5-d1pyridazin-4-one hydrochloride
• Example 200 2 - ((. 3-Amino-piperidin-1-vn-3- (but-2-indn-5- (4-chloronaphth-1-ylmethyl-3,5-dihydro-imidazof4.5-d1pyridazine-4 -one
• Example 201 2 ((r) -3-Amino-piperidin-1-yl) -3- (but-2-ynyl) -5- (4-bromonaphth-1-ylmethyl) -3,5-dihydroimidazorone, 4,5 -d1pyridazin-4-one hydrochloride
• Example 204 2 (() -3-Amino-piperidin-1-yl) -3- (but-2-ynyl) -5- (benzori .2,5-thiadiazol-5-ylmethyl) -3,5-dihydro-imidazof4.5- dlpyridazin-on-4
• Example 206 2 ((V3-Amino-piperidine-1-v ⁇ -3- (but-2-ind-5- (2-methyl-naphthalen-1-ylmethyl) -5,5-dihydro-imidazor4,5-d1pyridazine-4 -one
• Example 208 2 ((r?) - 3-Amino-piperidin-1-vn-3- (but-2-indn-5- (isoquinolin-3-ylmethyl) -3,5-dihydroimidazor4,5-dlpyridazine-4 -one
• Example 210 2 - ((R) -3-Amino-piperidin-1-vn-3- (but-2-ynyl) -5- (2-methyl-2 / - / - indazol-3-ylmethane-3,5-dihydrodro -imidazof4.5-d1pyridazin-4-one
• Example 212 2 - ((R) -3-Amino-piperidin-1-yl) -3- ⁇ / but-2- ⁇ -7-methyl-5- (4-methyl-quinazolin-2-ylmethyl) -3.5- dihydro-imidazo [4,5-d1pyridazin-4-one
• HPLC analysis was carried out under the following experimental conditions: column: Xterra MS18; 3.5 ⁇ m; 4.6 x 50 mm flow: 1 mL / min
• Plasticizer A water / 0.1% trifluoroacetic acid
• eluant B acetonitrile / 0.1% trifluoroacetic acid
• Example 215 2 - ((R) -3-Amino-piperidin-1-vn-3- (but-2-ynyl) -5- (4-methyl-phthalazin-1-ylmethane-3,5-dihydroidazo r 4.5-dlpyridazin-on-4
• Example 221 2 - (() -3-Amino-piperidin-1-yl) -3- (but-2-ynyl) -5- (4-morpholin-4-yl-quinazolin-2-ylmethyl) -3.5 -dihvdro-imidazor4,5-dlpyridazin-4-one
• Example 223 2 - (() -3-Amino-piperidin-1-yl) -3- (but-2-ynyl) -7-methyl-5- (4-cyanopnaphthalen-1-ylmethyl) -3.5-dihydro-d -imidazof4.5-dlpyridazin-4-one
• 1 drag core contains:
• the active substance is mixed with calcium phosphate, corn starch, polyvinylpyrrolidone, hydroxypropylmethylcellulose and half of the stated amount of magnesium stearate.
• a tableting machine compacts are produced with a diameter of about 13 mm, these are ground on a suitable machine through a sieve with 1, 5 mm mesh size and mixed with the remaining amount of magnesium stearate. This granulate is pressed on a tabletting machine into tablets of the desired shape. Core weight: 230 mg. Stamp: 9 mm, curved
• the coated dragee cores are coated with a film consisting essentially of hydroxypropylmethylcellulose.
• the finished film dragees are shined with beeswax. Dragee weight: 245 mg.
• 1 tablet contains:
• Production method Active ingredient, milk sugar and starch are mixed and uniformly moistened with an aqueous solution of polyvinylpyrrolidone. After sieving the moist mass (2.0 mm mesh size) and drying in a rack drying oven at 50 ° C is sieved again (1, 5 mm mesh size) and the lubricant mixed. The ready-to-use mixture is processed into tablets.
• Diameter 10 mm, biplan with facet on both sides and one-sided part notch.
• 1 tablet contains:
• Production method The active substance mixed with milk sugar, corn starch and silicic acid is moistened with a 20% strength aqueous solution of polyvinylpyrrolidone and beaten through a sieve with a mesh size of 1.5 mm.
• the granules dried at 45 ° C are again rubbed through the same sieve and mixed with the stated amount of magnesium stearate. From the mixture tablets are pressed.
• 1 capsule contains: active ingredient 150.0 mg
• Corn starch drink about 180.0 mg
• the active ingredient is mixed with the excipients, passed through a sieve of 0.75 mm mesh size and mixed homogeneously in a suitable device.
• the final mixture is filled into size 1 hard gelatin capsules.
• Capsule shell hard gelatin capsule size 1.
• Example 228 suppositories with 150 mq active substance
• 1 suppository contains:
• Polyethylene glycol 1500 550.0 mg
• 100 ml suspension contain: active ingredient 1, 00 g
• Carboxymethylcellulose Na salt 0.10 g of methyl p-hydroxybenzoate 0.05 g of propyl p-hydroxybenzoate 0.01 g
• Dest. Water is heated to 70 ° C.
• p-hydroxybenzoic acid methyl ester and propyl ester and also glycerol and carboxymethylcellulose sodium salt are dissolved with stirring. It is cooled to room temperature and added with stirring, the active ingredient and dispersed homogeneously. After addition and dissolution of the sugar, the sorbitol solution and the aroma, the suspension is evacuated to vent with stirring. 5 ml of suspension contain 50 mg of active ingredient.
• Active ingredient 10.0 mg 0.01 N hydrochloric acid s.q.
• the active substance is dissolved in the required amount of 0.01 N HCl, made isotonic with sodium chloride, sterile-filtered and filled into 2 ml ampoules.
• Active ingredient 50.0 mg 0.01 N hydrochloric acid s.q.
• the active ingredient is dissolved in the required amount of 0.01 N HCl, made isotonic with sodium chloride, sterile filtered and filled into 10 ml ampoules.

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