UA75632C2 - СПОСІБ ЕКСТРАКЦІЇ β-АМІЛАЗИ - Google Patents
СПОСІБ ЕКСТРАКЦІЇ β-АМІЛАЗИ Download PDFInfo
- Publication number
- UA75632C2 UA75632C2 UA2003087452A UA2003087452A UA75632C2 UA 75632 C2 UA75632 C2 UA 75632C2 UA 2003087452 A UA2003087452 A UA 2003087452A UA 2003087452 A UA2003087452 A UA 2003087452A UA 75632 C2 UA75632 C2 UA 75632C2
- Authority
- UA
- Ukraine
- Prior art keywords
- amylase
- cellulase
- grain
- extraction
- extracted
- Prior art date
Links
- 238000000605 extraction Methods 0.000 title claims abstract description 55
- 239000004382 Amylase Substances 0.000 title claims description 65
- 108010059892 Cellulase Proteins 0.000 claims abstract description 57
- 229940106157 cellulase Drugs 0.000 claims abstract description 56
- 230000000694 effects Effects 0.000 claims abstract description 45
- 239000000284 extract Substances 0.000 claims abstract description 32
- 230000002255 enzymatic effect Effects 0.000 claims abstract description 7
- 108010002430 hemicellulase Proteins 0.000 claims abstract description 7
- 229940059442 hemicellulase Drugs 0.000 claims abstract description 6
- 239000012736 aqueous medium Substances 0.000 claims abstract description 5
- 235000013339 cereals Nutrition 0.000 claims description 59
- 238000000034 method Methods 0.000 claims description 32
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 31
- 241000209219 Hordeum Species 0.000 claims description 21
- 235000007340 Hordeum vulgare Nutrition 0.000 claims description 21
- 241000209140 Triticum Species 0.000 claims description 14
- 235000021307 Triticum Nutrition 0.000 claims description 14
- 230000001603 reducing effect Effects 0.000 claims description 12
- 238000002360 preparation method Methods 0.000 claims description 9
- 238000000227 grinding Methods 0.000 claims description 5
- 238000000746 purification Methods 0.000 claims description 5
- 235000007238 Secale cereale Nutrition 0.000 claims description 4
- 238000000926 separation method Methods 0.000 claims description 3
- 241000209056 Secale Species 0.000 claims description 2
- 101710172711 Structural protein Proteins 0.000 claims description 2
- 238000004140 cleaning Methods 0.000 claims description 2
- 238000005498 polishing Methods 0.000 claims description 2
- 239000004464 cereal grain Substances 0.000 claims 2
- 244000005700 microbiome Species 0.000 claims 1
- 108010019077 beta-Amylase Proteins 0.000 abstract description 8
- 101710130006 Beta-glucanase Proteins 0.000 abstract 2
- 239000003795 chemical substances by application Substances 0.000 abstract 2
- 238000002955 isolation Methods 0.000 abstract 1
- 239000002609 medium Substances 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 description 24
- 108090000790 Enzymes Proteins 0.000 description 24
- 229940088598 enzyme Drugs 0.000 description 24
- 229920002472 Starch Polymers 0.000 description 23
- 235000019698 starch Nutrition 0.000 description 22
- 239000008107 starch Substances 0.000 description 22
- 229920001503 Glucan Polymers 0.000 description 14
- GEHJYWRUCIMESM-UHFFFAOYSA-L sodium sulfite Chemical compound [Na+].[Na+].[O-]S([O-])=O GEHJYWRUCIMESM-UHFFFAOYSA-L 0.000 description 14
- 108010084185 Cellulases Proteins 0.000 description 11
- 102000005575 Cellulases Human genes 0.000 description 11
- 239000008186 active pharmaceutical agent Substances 0.000 description 9
- 239000001913 cellulose Substances 0.000 description 9
- 229920002678 cellulose Polymers 0.000 description 9
- 239000000243 solution Substances 0.000 description 9
- 238000004519 manufacturing process Methods 0.000 description 8
- 239000000203 mixture Substances 0.000 description 8
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 7
- 241000894006 Bacteria Species 0.000 description 7
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 7
- 238000012545 processing Methods 0.000 description 7
- 235000010265 sodium sulphite Nutrition 0.000 description 7
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 6
- 239000008103 glucose Substances 0.000 description 6
- NLKNQRATVPKPDG-UHFFFAOYSA-M potassium iodide Chemical compound [K+].[I-] NLKNQRATVPKPDG-UHFFFAOYSA-M 0.000 description 6
- HRZFUMHJMZEROT-UHFFFAOYSA-L sodium disulfite Chemical compound [Na+].[Na+].[O-]S(=O)S([O-])(=O)=O HRZFUMHJMZEROT-UHFFFAOYSA-L 0.000 description 6
- 235000010262 sodium metabisulphite Nutrition 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 108010008885 Cellulose 1,4-beta-Cellobiosidase Proteins 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 5
- 239000000523 sample Substances 0.000 description 5
- 229940001584 sodium metabisulfite Drugs 0.000 description 5
- 239000000758 substrate Substances 0.000 description 5
- GUBGYTABKSRVRQ-CUHNMECISA-N D-Cellobiose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-CUHNMECISA-N 0.000 description 4
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 description 4
- 229920002488 Hemicellulose Polymers 0.000 description 4
- 239000012153 distilled water Substances 0.000 description 4
- 229940079593 drug Drugs 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- 235000013312 flour Nutrition 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 101710112457 Exoglucanase Proteins 0.000 description 3
- 241000233866 Fungi Species 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 108010050181 aleurone Proteins 0.000 description 3
- 229940025131 amylases Drugs 0.000 description 3
- -1 arabinans Chemical class 0.000 description 3
- 108010047754 beta-Glucosidase Proteins 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- YAGKRVSRTSUGEY-UHFFFAOYSA-N ferricyanide Chemical compound [Fe+3].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-] YAGKRVSRTSUGEY-UHFFFAOYSA-N 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 229920001282 polysaccharide Polymers 0.000 description 3
- 239000005017 polysaccharide Substances 0.000 description 3
- 150000004804 polysaccharides Chemical class 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 235000000346 sugar Nutrition 0.000 description 3
- 238000004448 titration Methods 0.000 description 3
- 108010013043 Acetylesterase Proteins 0.000 description 2
- 229920000945 Amylopectin Polymers 0.000 description 2
- 229920000856 Amylose Polymers 0.000 description 2
- 244000068988 Glycine max Species 0.000 description 2
- 235000010469 Glycine max Nutrition 0.000 description 2
- 102100036617 Monoacylglycerol lipase ABHD2 Human genes 0.000 description 2
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 2
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
- 102000006995 beta-Glucosidase Human genes 0.000 description 2
- 238000009835 boiling Methods 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 235000009508 confectionery Nutrition 0.000 description 2
- 238000000354 decomposition reaction Methods 0.000 description 2
- 238000007865 diluting Methods 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- 239000010808 liquid waste Substances 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 235000018102 proteins Nutrition 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 150000008163 sugars Chemical class 0.000 description 2
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 description 1
- DBTMGCOVALSLOR-UHFFFAOYSA-N 32-alpha-galactosyl-3-alpha-galactosyl-galactose Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(OC2C(C(CO)OC(O)C2O)O)OC(CO)C1O DBTMGCOVALSLOR-UHFFFAOYSA-N 0.000 description 1
- 235000007319 Avena orientalis Nutrition 0.000 description 1
- 244000075850 Avena orientalis Species 0.000 description 1
- 208000023514 Barrett esophagus Diseases 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- RXVWSYJTUUKTEA-UHFFFAOYSA-N D-maltotriose Natural products OC1C(O)C(OC(C(O)CO)C(O)C(O)C=O)OC(CO)C1OC1C(O)C(O)C(O)C(CO)O1 RXVWSYJTUUKTEA-UHFFFAOYSA-N 0.000 description 1
- 229920001353 Dextrin Polymers 0.000 description 1
- 239000004375 Dextrin Substances 0.000 description 1
- 239000001653 FEMA 3120 Substances 0.000 description 1
- 108010068370 Glutens Proteins 0.000 description 1
- 108010073032 Grain Proteins Proteins 0.000 description 1
- 229920000057 Mannan Polymers 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 229920001131 Pulp (paper) Polymers 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- PDMMFKSKQVNJMI-BLQWBTBKSA-N Testosterone propionate Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H](OC(=O)CC)[C@@]1(C)CC2 PDMMFKSKQVNJMI-BLQWBTBKSA-N 0.000 description 1
- 244000295923 Yucca aloifolia Species 0.000 description 1
- 235000004552 Yucca aloifolia Nutrition 0.000 description 1
- 235000012044 Yucca brevifolia Nutrition 0.000 description 1
- 235000017049 Yucca glauca Nutrition 0.000 description 1
- 239000008351 acetate buffer Substances 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 235000013405 beer Nutrition 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 125000002057 carboxymethyl group Chemical group [H]OC(=O)C([H])([H])[*] 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 239000000287 crude extract Substances 0.000 description 1
- 235000019425 dextrin Nutrition 0.000 description 1
- 230000002478 diastatic effect Effects 0.000 description 1
- 229940079919 digestives enzyme preparation Drugs 0.000 description 1
- 239000012470 diluted sample Substances 0.000 description 1
- 108010091371 endoglucanase 1 Proteins 0.000 description 1
- 108010091384 endoglucanase 2 Proteins 0.000 description 1
- 108010092450 endoglucanase Z Proteins 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000012812 general test Methods 0.000 description 1
- 150000002304 glucoses Chemical class 0.000 description 1
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 1
- 235000021312 gluten Nutrition 0.000 description 1
- 239000010903 husk Substances 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 108010076363 licheninase Proteins 0.000 description 1
- 210000003041 ligament Anatomy 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- LUEWUZLMQUOBSB-GFVSVBBRSA-N mannan Chemical class O[C@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@@H](O[C@@H]2[C@H](O[C@@H](O[C@H]3[C@H](O[C@@H](O)[C@@H](O)[C@H]3O)CO)[C@@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O LUEWUZLMQUOBSB-GFVSVBBRSA-N 0.000 description 1
- FYGDTMLNYKFZSV-UHFFFAOYSA-N mannotriose Natural products OC1C(O)C(O)C(CO)OC1OC1C(CO)OC(OC2C(OC(O)C(O)C2O)CO)C(O)C1O FYGDTMLNYKFZSV-UHFFFAOYSA-N 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 150000002972 pentoses Chemical class 0.000 description 1
- 210000003516 pericardium Anatomy 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 239000012898 sample dilution Substances 0.000 description 1
- 235000010413 sodium alginate Nutrition 0.000 description 1
- 239000000661 sodium alginate Substances 0.000 description 1
- 229940005550 sodium alginate Drugs 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- 239000004296 sodium metabisulphite Substances 0.000 description 1
- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
- 235000011152 sodium sulphate Nutrition 0.000 description 1
- SETMGIIITGNLAS-UHFFFAOYSA-N spizofurone Chemical compound O=C1C2=CC(C(=O)C)=CC=C2OC21CC2 SETMGIIITGNLAS-UHFFFAOYSA-N 0.000 description 1
- 229950001870 spizofurone Drugs 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- 210000001550 testis Anatomy 0.000 description 1
- 239000004753 textile Substances 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
- 229940100445 wheat starch Drugs 0.000 description 1
- 235000020985 whole grains Nutrition 0.000 description 1
- 229920001221 xylan Polymers 0.000 description 1
- 150000004823 xylans Chemical class 0.000 description 1
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 1
- 229960001763 zinc sulfate Drugs 0.000 description 1
- 229910000368 zinc sulfate Inorganic materials 0.000 description 1
- FYGDTMLNYKFZSV-BYLHFPJWSA-N β-1,4-galactotrioside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@H](CO)O[C@@H](O[C@@H]2[C@@H](O[C@@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-BYLHFPJWSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2422—Alpha-amylase (3.2.1.1.) from plant source
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2425—Beta-amylase (3.2.1.2)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Enzymes And Modification Thereof (AREA)
- Cereal-Derived Products (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Plant Substances (AREA)
- Extraction Or Liquid Replacement (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FI20010221A FI109358B (fi) | 2001-02-06 | 2001-02-06 | Menetelmä entsyymin uuttamiseksi |
| PCT/FI2002/000070 WO2002062980A1 (en) | 2001-02-06 | 2002-01-30 | Process for the extraction of beta-amylase |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| UA75632C2 true UA75632C2 (uk) | 2006-05-15 |
Family
ID=8560253
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| UA2003087452A UA75632C2 (uk) | 2001-02-06 | 2002-01-30 | СПОСІБ ЕКСТРАКЦІЇ β-АМІЛАЗИ |
Country Status (23)
| Country | Link |
|---|---|
| US (1) | US7399622B2 (es) |
| EP (1) | EP1363999B1 (es) |
| JP (1) | JP4092689B2 (es) |
| KR (1) | KR100483501B1 (es) |
| CN (1) | CN1228444C (es) |
| AR (1) | AR035221A1 (es) |
| AT (1) | ATE323155T1 (es) |
| AU (1) | AU783947B2 (es) |
| CA (1) | CA2370336C (es) |
| CY (1) | CY1107469T1 (es) |
| CZ (1) | CZ303447B6 (es) |
| DE (1) | DE60210586T2 (es) |
| DK (1) | DK1363999T3 (es) |
| EE (1) | EE05410B1 (es) |
| ES (1) | ES2261644T3 (es) |
| FI (1) | FI109358B (es) |
| FR (1) | FR2820433A1 (es) |
| NZ (1) | NZ527055A (es) |
| PT (1) | PT1363999E (es) |
| RU (1) | RU2290440C2 (es) |
| UA (1) | UA75632C2 (es) |
| WO (1) | WO2002062980A1 (es) |
| ZA (1) | ZA200305605B (es) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2943686B1 (fr) * | 2009-03-30 | 2013-11-01 | Roquette Freres | Procede d'obtention d'une preparation de beta-amylases a partir des fractions solubles de plantes amidonnieres |
| SG193604A1 (en) * | 2011-03-24 | 2013-10-30 | Nestec Sa | Method for providing a whole grain cereal based extract |
| FR2994440B1 (fr) * | 2012-08-07 | 2020-01-31 | Roquette Freres | Procede d'extraction de beta-amylases a partir d'une fraction soluble de plante amidonniere et en presence d'une protease |
| CN110184257B (zh) * | 2019-07-23 | 2019-11-12 | 烟台麦特尔生物技术有限公司 | 一种大麦β-淀粉酶提取工艺 |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US647538A (en) * | 1898-03-21 | 1900-04-17 | James P Taylor | Bicycle-support. |
| US3492203A (en) * | 1965-07-08 | 1970-01-27 | Hayashibara Co | Extraction of beta-amylase from wheat bran |
| FI61516C (fi) | 1980-10-09 | 1982-08-10 | Suomen Nestesokeri Oy | Foerfarande foer extrahering av beta-amylas ur saedeskorn |
| US4675296A (en) * | 1982-01-18 | 1987-06-23 | Suomen Sokeri Oy | Process for the extraction of β-amylase from barley grains |
| JPS6018393B2 (ja) | 1983-07-27 | 1985-05-10 | グリコ栄養食品株式会社 | 小麦でんぷん製造廃液からβアミラ−ゼを回収する方法 |
| JPS60126080A (ja) | 1983-12-08 | 1985-07-05 | Amano Pharmaceut Co Ltd | β−アミラ−ゼの製造法 |
| US4647538A (en) | 1984-09-18 | 1987-03-03 | Michigan Biotechnology Institute | Thermostable beta-amylase |
| DK160563C (da) | 1986-02-19 | 1991-09-02 | Novo Industri As | Beta-amylase, fremgangsmaade til dens fremstilling samt praeparat indeholdende beta-amylasen |
| JPS6379590A (ja) | 1986-09-24 | 1988-04-09 | Glyco Eiyou Shokuhin Kk | β−アミラ−ゼを製造する方法 |
| JPH0236231B2 (ja) | 1987-04-02 | 1990-08-16 | Ueda Kagaku Kogyo Kk | Komugibeetaaamiraazezainoseizohoho |
| US5066218A (en) | 1987-05-13 | 1991-11-19 | Genencor International, Inc. | Composition of a steeped starched-containing grain and a cellulase enzyme |
| NL8702735A (nl) * | 1987-11-17 | 1989-06-16 | Dorr Oliver Inc | Werkwijze voor het weken van granen met een nieuw enzympreparaat. |
| FI79858C (fi) | 1988-05-10 | 1990-03-12 | Alko Ab Oy | Foerfarande foer extrahering av enzymer ur spannmaol. |
| JPH0740937B2 (ja) | 1992-02-07 | 1995-05-10 | 農林水産省食品総合研究所長 | β−アミラーゼの分離法 |
-
2001
- 2001-02-06 FI FI20010221A patent/FI109358B/fi not_active IP Right Cessation
- 2001-12-28 AU AU97513/01A patent/AU783947B2/en not_active Ceased
-
2002
- 2002-01-23 AR ARP020100221A patent/AR035221A1/es active IP Right Grant
- 2002-01-30 DK DK02710923T patent/DK1363999T3/da active
- 2002-01-30 WO PCT/FI2002/000070 patent/WO2002062980A1/en active IP Right Grant
- 2002-01-30 DE DE60210586T patent/DE60210586T2/de not_active Expired - Lifetime
- 2002-01-30 JP JP2002563317A patent/JP4092689B2/ja not_active Expired - Fee Related
- 2002-01-30 CZ CZ20032036A patent/CZ303447B6/cs not_active IP Right Cessation
- 2002-01-30 EE EEP200300354A patent/EE05410B1/xx not_active IP Right Cessation
- 2002-01-30 EP EP02710923A patent/EP1363999B1/en not_active Expired - Lifetime
- 2002-01-30 RU RU2003127065/13A patent/RU2290440C2/ru not_active IP Right Cessation
- 2002-01-30 UA UA2003087452A patent/UA75632C2/uk unknown
- 2002-01-30 PT PT02710923T patent/PT1363999E/pt unknown
- 2002-01-30 NZ NZ527055A patent/NZ527055A/en not_active IP Right Cessation
- 2002-01-30 ES ES02710923T patent/ES2261644T3/es not_active Expired - Lifetime
- 2002-01-30 AT AT02710923T patent/ATE323155T1/de active
- 2002-01-30 CN CNB028046021A patent/CN1228444C/zh not_active Expired - Fee Related
- 2002-01-31 FR FR0201125A patent/FR2820433A1/fr active Pending
- 2002-02-04 CA CA2370336A patent/CA2370336C/en not_active Expired - Fee Related
- 2002-02-04 KR KR10-2002-0006130A patent/KR100483501B1/ko not_active IP Right Cessation
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2003
- 2003-07-21 ZA ZA200305605A patent/ZA200305605B/en unknown
- 2003-08-06 US US10/635,183 patent/US7399622B2/en not_active Expired - Fee Related
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2006
- 2006-07-06 CY CY20061100931T patent/CY1107469T1/el unknown
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