TWI327596B - - Google Patents

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Publication number
TWI327596B
TWI327596B TW094143462A TW94143462A TWI327596B TW I327596 B TWI327596 B TW I327596B TW 094143462 A TW094143462 A TW 094143462A TW 94143462 A TW94143462 A TW 94143462A TW I327596 B TWI327596 B TW I327596B
Authority
TW
Taiwan
Prior art keywords
nitrilase
sequence
subunit
strain
amino acid
Prior art date
Application number
TW094143462A
Other languages
English (en)
Chinese (zh)
Other versions
TW200634151A (en
Inventor
Kaoru Furuya
Akira Tamaki
Shin-Ichiro Nagasawa
Ayano Suzuki
Original Assignee
Asahi Chemical Ind
Tongsuh Petrochemical Corp Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Asahi Chemical Ind, Tongsuh Petrochemical Corp Ltd filed Critical Asahi Chemical Ind
Publication of TW200634151A publication Critical patent/TW200634151A/zh
Application granted granted Critical
Publication of TWI327596B publication Critical patent/TWI327596B/zh

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/02—Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
TW094143462A 2004-12-09 2005-12-08 Transformant expressing nitrile hydratase TW200634151A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2004357035 2004-12-09

Publications (2)

Publication Number Publication Date
TW200634151A TW200634151A (en) 2006-10-01
TWI327596B true TWI327596B (enExample) 2010-07-21

Family

ID=36578009

Family Applications (1)

Application Number Title Priority Date Filing Date
TW094143462A TW200634151A (en) 2004-12-09 2005-12-08 Transformant expressing nitrile hydratase

Country Status (4)

Country Link
JP (1) JPWO2006062189A1 (enExample)
KR (1) KR20070085922A (enExample)
TW (1) TW200634151A (enExample)
WO (1) WO2006062189A1 (enExample)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5080787B2 (ja) * 2006-11-15 2012-11-21 達彦 小林 タンパク質改変方法、ニトリルヒドラターゼ成熟化方法、成熟化ニトリルヒドラターゼ生産方法、成熟化ニトリルヒドラターゼを用いたアミド化合物生産方法
JP5817088B2 (ja) * 2010-03-24 2015-11-18 三菱レイヨン株式会社 ニトリルヒドラターゼ遺伝子を欠失又は不活性化させた微生物
JP2011217665A (ja) * 2010-04-08 2011-11-04 Mitsubishi Rayon Co Ltd ニトリルヒドラターゼ遺伝子を置換した微生物
BR112014020899A2 (pt) 2012-02-28 2018-05-02 Mitsubishi Rayon Co., Ltd. métodos para produzir, preservar, e ativar nitrila hidratase
JP6156442B2 (ja) * 2015-05-28 2017-07-05 三菱ケミカル株式会社 ニトリルヒドラターゼ遺伝子を置換した微生物
EP3564368B1 (en) * 2016-12-28 2025-03-26 Mitsui Chemicals, Inc. Mutant nitrile hydratase, nucleic acid coding said mutant nitrile hydratase, expression vector and transformant including said nucleic acid, production method for said mutant nitrile hydratase, and production method for amide compound
EP3710592B1 (en) 2017-11-14 2022-08-31 Columbia S.r.l. Microbiological process for the preparation of amides
CN111334495B (zh) * 2020-03-12 2022-04-29 宜昌东阳光生化制药有限公司 制备右旋酰胺酮洛芬的方法
CN119220525B (zh) * 2024-09-23 2025-11-21 浙江工业大学 腈水解酶突变体及其在饲料中的应用

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63137688A (ja) * 1986-12-01 1988-06-09 Res Assoc Util Of Light Oil アミド化合物の製造法
JPH0753104B2 (ja) * 1989-02-28 1995-06-07 秀明 山田 細菌の培養法
FR2708936B1 (fr) * 1993-08-10 1995-11-10 Rhone Poulenc Chimie Enzymes à activité nitrile-hydratase, outils génétiques et micro-organismes hôtes permettant leur obtention et procédé d'hydrolyse mettant en Óoeuvre lesdites enzymes.
JP3763157B2 (ja) * 1996-03-18 2006-04-05 住友化学株式会社 ニトリルヒドラターゼ、その遺伝子及びその利用
US6153415A (en) * 1998-04-29 2000-11-28 Board Of Trustees Operating Michigan State University Method for producing amide compounds using a nitrile hydratase from a thermophilic bacillus
KR100320038B1 (ko) * 1999-11-19 2002-02-01 이상현 로도코커스 로도크로스(Ｒｈｏｄｏｃｏｃｃｕｓｒｈｏｄｏｃｈｒｏｕｓ) Ｍ33 ＶＫＭ Ａｃ-1515Ｄ로부터유래된 니트릴 히드라타제를 코딩하는 유전자 및 이유전자를 함유한 형질전환체
CN1806047A (zh) * 2003-06-10 2006-07-19 旭化成株式会社 新型腈水合酶

Also Published As

Publication number Publication date
WO2006062189A1 (ja) 2006-06-15
TW200634151A (en) 2006-10-01
JPWO2006062189A1 (ja) 2008-06-12
KR20070085922A (ko) 2007-08-27

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MM4A Annulment or lapse of patent due to non-payment of fees