KR20070085922A - 니트릴히드라타제를 발현하는 형질 전환체 - Google Patents

니트릴히드라타제를 발현하는 형질 전환체 Download PDF

Info

Publication number
KR20070085922A
KR20070085922A KR1020077012947A KR20077012947A KR20070085922A KR 20070085922 A KR20070085922 A KR 20070085922A KR 1020077012947 A KR1020077012947 A KR 1020077012947A KR 20077012947 A KR20077012947 A KR 20077012947A KR 20070085922 A KR20070085922 A KR 20070085922A
Authority
KR
South Korea
Prior art keywords
nitrile hydratase
subunit
sequence
dna
seq
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
KR1020077012947A
Other languages
English (en)
Korean (ko)
Inventor
가오루 후루야
아끼라 다마끼
신이찌로 나가사와
아야노 스즈끼
Original Assignee
아사히 가세이 가부시키가이샤
동서석유화학주식회사
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 아사히 가세이 가부시키가이샤, 동서석유화학주식회사 filed Critical 아사히 가세이 가부시키가이샤
Publication of KR20070085922A publication Critical patent/KR20070085922A/ko
Ceased legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/02—Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
KR1020077012947A 2004-12-09 2005-12-09 니트릴히드라타제를 발현하는 형질 전환체 Ceased KR20070085922A (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JPJP-P-2004-00357035 2004-12-09
JP2004357035 2004-12-09

Publications (1)

Publication Number Publication Date
KR20070085922A true KR20070085922A (ko) 2007-08-27

Family

ID=36578009

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020077012947A Ceased KR20070085922A (ko) 2004-12-09 2005-12-09 니트릴히드라타제를 발현하는 형질 전환체

Country Status (4)

Country Link
JP (1) JPWO2006062189A1 (enExample)
KR (1) KR20070085922A (enExample)
TW (1) TW200634151A (enExample)
WO (1) WO2006062189A1 (enExample)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5080787B2 (ja) * 2006-11-15 2012-11-21 達彦 小林 タンパク質改変方法、ニトリルヒドラターゼ成熟化方法、成熟化ニトリルヒドラターゼ生産方法、成熟化ニトリルヒドラターゼを用いたアミド化合物生産方法
JP5817088B2 (ja) * 2010-03-24 2015-11-18 三菱レイヨン株式会社 ニトリルヒドラターゼ遺伝子を欠失又は不活性化させた微生物
JP2011217665A (ja) * 2010-04-08 2011-11-04 Mitsubishi Rayon Co Ltd ニトリルヒドラターゼ遺伝子を置換した微生物
BR112014020899A2 (pt) 2012-02-28 2018-05-02 Mitsubishi Rayon Co., Ltd. métodos para produzir, preservar, e ativar nitrila hidratase
JP6156442B2 (ja) * 2015-05-28 2017-07-05 三菱ケミカル株式会社 ニトリルヒドラターゼ遺伝子を置換した微生物
EP3564368B1 (en) * 2016-12-28 2025-03-26 Mitsui Chemicals, Inc. Mutant nitrile hydratase, nucleic acid coding said mutant nitrile hydratase, expression vector and transformant including said nucleic acid, production method for said mutant nitrile hydratase, and production method for amide compound
EP3710592B1 (en) 2017-11-14 2022-08-31 Columbia S.r.l. Microbiological process for the preparation of amides
CN111334495B (zh) * 2020-03-12 2022-04-29 宜昌东阳光生化制药有限公司 制备右旋酰胺酮洛芬的方法
CN119220525B (zh) * 2024-09-23 2025-11-21 浙江工业大学 腈水解酶突变体及其在饲料中的应用

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63137688A (ja) * 1986-12-01 1988-06-09 Res Assoc Util Of Light Oil アミド化合物の製造法
JPH0753104B2 (ja) * 1989-02-28 1995-06-07 秀明 山田 細菌の培養法
FR2708936B1 (fr) * 1993-08-10 1995-11-10 Rhone Poulenc Chimie Enzymes à activité nitrile-hydratase, outils génétiques et micro-organismes hôtes permettant leur obtention et procédé d'hydrolyse mettant en Óoeuvre lesdites enzymes.
JP3763157B2 (ja) * 1996-03-18 2006-04-05 住友化学株式会社 ニトリルヒドラターゼ、その遺伝子及びその利用
US6153415A (en) * 1998-04-29 2000-11-28 Board Of Trustees Operating Michigan State University Method for producing amide compounds using a nitrile hydratase from a thermophilic bacillus
KR100320038B1 (ko) * 1999-11-19 2002-02-01 이상현 로도코커스 로도크로스(Ｒｈｏｄｏｃｏｃｃｕｓｒｈｏｄｏｃｈｒｏｕｓ) Ｍ33 ＶＫＭ Ａｃ-1515Ｄ로부터유래된 니트릴 히드라타제를 코딩하는 유전자 및 이유전자를 함유한 형질전환체
CN1806047A (zh) * 2003-06-10 2006-07-19 旭化成株式会社 新型腈水合酶

Also Published As

Publication number Publication date
WO2006062189A1 (ja) 2006-06-15
TW200634151A (en) 2006-10-01
JPWO2006062189A1 (ja) 2008-06-12
TWI327596B (enExample) 2010-07-21

Similar Documents

Publication Publication Date Title
JP5069032B2 (ja) 改良型ニトリルヒドラターゼ
KR20100127887A (ko) 실로-이노시톨의 제조방법
JP6048850B2 (ja) Ｄ−サクシニラーゼ、およびこれを用いたｄ−アミノ酸の製造方法
KR20070085922A (ko) 니트릴히드라타제를 발현하는 형질 전환체
CN106062188B (zh) 具有提高的转化活性的l-阿拉伯糖异构酶变异体及利用该变异体生产d-塔格糖的方法
JP5903298B2 (ja) Ｎ−サクシニル−ｄｌ−アミノ酸に対する向上されたｄ体選択性を有する改変型ｄ−サクシニラーゼ
KR100806991B1 (ko) 신규 니트릴 히드라타아제
KR100244066B1 (ko) D-N-카르바모일-α-아미노산의 제조법
EP1365023A1 (en) Dna encoding novel d-aminoacylase and process for producing d-amino acid by using the same
JP4489598B2 (ja) Ｄ−アミノアシラーゼ
AU773130B2 (en) Method for isolating and selecting genes coding for enzymes, and suitable culture medium
KR100270508B1 (ko) 신규세파로스포린디아세틸라아제유전자및이를이용한단백질제조방법
JP2006055131A (ja) 新規なｄ−アミノアシラーゼおよびその遺伝子
CN1989246B (zh) L-氨基酸酰胺不对称水解酶及其编码dna
KR20030006724A (ko) 신규한 ｄ-입체특이적 아미노산 아미다아제, 그의 유전자,그의 제조방법, 이를 이용한 ｄ-아미노산의 제조방법
US7655450B2 (en) Polypeptide having amidase activity and gene thereof
KR100500978B1 (ko) 에틸렌디아민-n,n'-디숙신산:에틸렌디아민리아제활성을가진단백질및그를암호화하는유전자
JP3930555B2 (ja) ３−ケト−アシルＣｏＡリダクターゼ及びそれをコードする遺伝子
JP2003125784A (ja) 新規チロシン脱炭酸酵素
JP2003061692A (ja) 活性化されたｒｅｃ−ヒダントイナーゼの製造方法、該方法で得られるｒｅｃ−ヒダントイナーゼ、これをコードする核酸、それを有するプラスミド、ベクター及び微生物、ハイブリダイズする核酸、その製造のためのプライマー並びにｒｅｃ−ヒダントイナーゼ及び核酸の使用

Legal Events

Date Code Title Description
A201 Request for examination
AMND Amendment
PA0105 International application

Patent event date: 20070608

Patent event code: PA01051R01D

Comment text: International Patent Application

PA0201 Request for examination
PG1501 Laying open of application
E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 20081120

Patent event code: PE09021S01D

AMND Amendment
E601 Decision to refuse application
PE0601 Decision on rejection of patent

Patent event date: 20100311

Comment text: Decision to Refuse Application

Patent event code: PE06012S01D

Patent event date: 20081120

Comment text: Notification of reason for refusal

Patent event code: PE06011S01I

AMND Amendment
J201 Request for trial against refusal decision
PJ0201 Trial against decision of rejection

Patent event date: 20100610

Comment text: Request for Trial against Decision on Refusal

Patent event code: PJ02012R01D

Patent event date: 20100311

Comment text: Decision to Refuse Application

Patent event code: PJ02011S01I

Appeal kind category: Appeal against decision to decline refusal

Decision date: 20110812

Appeal identifier: 2010101004292

Request date: 20100610

PB0901 Examination by re-examination before a trial

Comment text: Amendment to Specification, etc.

Patent event date: 20100610

Patent event code: PB09011R02I

Comment text: Request for Trial against Decision on Refusal

Patent event date: 20100610

Patent event code: PB09011R01I

Comment text: Amendment to Specification, etc.

Patent event date: 20090514

Patent event code: PB09011R02I

Comment text: Amendment to Specification, etc.

Patent event date: 20070608

Patent event code: PB09011R02I

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 20100812

Patent event code: PE09021S01D

B601 Maintenance of original decision after re-examination before a trial
PB0601 Maintenance of original decision after re-examination before a trial

Comment text: Report of Result of Re-examination before a Trial

Patent event code: PB06011S01D

Patent event date: 20110228

J301 Trial decision

Free format text: TRIAL DECISION FOR APPEAL AGAINST DECISION TO DECLINE REFUSAL REQUESTED 20100610

Effective date: 20110812

PJ1301 Trial decision

Patent event code: PJ13011S01D

Patent event date: 20110812

Comment text: Trial Decision on Objection to Decision on Refusal

Appeal kind category: Appeal against decision to decline refusal

Request date: 20100610

Decision date: 20110812

Appeal identifier: 2010101004292